F9 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	F9
Entrez Gene ID	14071
Full Name	coagulation factor IX
Synonyms	AW111646,Cf-9,Cf9
General protein information	Preferred Names coagulation factor IX Names coagulation factor IX christmas factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A6\|X 33.5 cM
Summary	This gene encodes a vitamin K-dependent serine protease that plays a critical role in the intrinsic pathway of blood coagulation. The encoded protein is an inactive zymogen that is activated by coagulation factor XIa to generate factor IXa, a heterodimer containing heavy and light chains. In association with factor VIII, membrane phospholipids and calcium ions, factor IXa cleaves the inactive zymogen factor X to generate active factor Xa. Genetic deletion of this gene in mice results in a severe bleeding phenotype. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305797.1	NP_001292726.1	coagulation factor IX isoform 2
NM_007979.2	NP_032005.1	coagulation factor IX isoform 1 preproprotein
XM_030251221.1	XP_030107081.1	coagulation factor IX isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	F9	NP_032005.1
Rattus norvegicus (Norway rat)	F9	NP_113728.1
	F9	NP_001103153.1
Gallus gallus (chicken)	F9	NP_989674.1
Danio rerio (zebrafish)	f9b	NP_001035400.1
Danio rerio (zebrafish)	f9a	NP_878288.1
Xenopus tropicalis (tropical clawed frog)	f9	NP_001011223.1
Homo sapiens (human)	F9	NP_000124.1
Pan troglodytes (chimpanzee)	F9	NP_001129063.1
Canis lupus familiaris (dog)	F9	NP_001003323.1

Gene Ontology
Bibliography

Related articles in PubMed

Targeting anticoagulant protein S to improve hemostasis in hemophilia.
Prince R, Bologna L, Manetti M, Melchiorre D, Rosa I, Dewarrat N, Suardi S, Amini P, Fern?ndez JA, Burnier L, Quarroz C, Reina Caro MD, Matsumura Y, Kremer Hovinga JA, Griffin JH, Simon HU, Ibba-Manneschi L, Saller F, Calzavarini S, Angelillo-Scherrer A
Blood(2018 Jan)

VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo.
Lacombe J, Rishavy MA, Berkner KL, Ferron M
JCI insight3(1)(2018 Jan)

Genome editing for scalable production of alloantigen-free lentiviral vectors for
Milani M, Annoni A, Bartolaccini S, Biffi M, Russo F, Di Tomaso T, Raimondi A, Lengler J, Holmes MC, Scheiflinger F, Lombardo A, Cantore A, Naldini L
EMBO molecular medicine9(11)1558-1573(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

activation peptide of factor IX regulates endothelial intercellular adhesion and thus could be used in the treatment of vascular hyperpermeability.
Title: Activation peptide of coagulation factor IX regulates endothelial permeability.

structural features within residues of the 39-loop contribute to the resistance of FIXa to inhibition by plasma inhibitors ZPI and TFPI.
Title: Residues of the 39-loop restrict the plasma inhibitor specificity of factor IXa.

Data indicate that the hyperactivating factor IX (FIX)-R338L mutation resulted in a robust 15-fold gain in potency and therefore provides a promising strategy to improve the efficacy, feasibility, and safety of hemophilia gene therapy.
Title: Hyperfunctional coagulation factor IX improves the efficacy of gene therapy in hemophilic mice.

hnRNP A3 is a major liver nuclear protein binding to age related increase element of the factor IX gene.
Title: Heterogeneous nuclear ribonucleoprotein A3 is the liver nuclear protein binding to age related increase element RNA of the factor IX gene.

functional role of binding of F9 to collagen IV in hemostasis.
Title: Abnormal hemostasis in a knock-in mouse carrying a variant of factor IX with impaired binding to collagen type IV.

The following F9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22121	NM_007979.2 Latest version!	Mus musculus coagulation factor IX (F9), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu67704	NM_001305797.1 Latest version!	Mus musculus coagulation factor IX (F9), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu90359	XM_030251221.1 Latest version!	Mus musculus coagulation factor IX (F9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F9 ( NM_007979.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032005.1 Mus musculus coagulation factor IX (F9), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22121
Clone ID Related Accession (Same CDS sequence)	NM_007979.2
Accession Version	NM_007979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	coagulation factor IX isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK149372.1 and AA239875.1. On Apr 3, 2015 this sequence version replaced NM_007979.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK149372.1, M23109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCACC TGAACACCGT CATGGCAGAA TCCCCGGCTC TCATCACCAT CTTCCTTTTA 
GGATATCTAC TCAGTACCGA ATGTGCAGTT TTCCTTGATC GTGAAAATGC CACCAAAATT 
CTTACCCGTC CAAAGAGATA TAATTCAGGA AAACTAGAAG AGTTTGTTCG AGGAAACCTT 
GAAAGAGAGT GTATAGAAGA AAGATGTAGT TTTGAAGAAG CACGAGAAGT TTTTGAAAAC 
ACTGAAAAAA CTACTGAATT TTGGAAGCAG TATGTTGATG GAGATCAGTG TGAATCAAAT 
CCTTGTTTAA ATGGTGGAAT ATGCAAGGAT GATATTAGTT CCTATGAATG CTGGTGCCAA 
GTTGGATTTG AAGGAAGGAA CTGTGAATTA GATGCAACGT GTAACATTAA AAATGGCAGG 
TGCAAGCAGT TTTGTAAAAA CAGTCCTGAT AACAAGGTAA TTTGTTCCTG CACTGAGGGA 
TACCAACTTG CAGAAGACCA GAAGTCCTGT GAACCAACAG TTCCATTTCC ATGTGGGAGA 
GCTTCTATTT CATACAGTTC TAAAAAGATC ACGAGAGCTG AGACTGTTTT CTCTAATATG 
GACTATGAAA ATTCTACTGA AGCTGTATTC ATTCAAGATG ACATCACTGA TGGTGCCATT 
CTTAATAACG TCACTGAAAG TAGTGAATCA CTTAATGACT TCACTCGAGT TGTTGGTGGA 
GAAAACGCAA AACCGGGTCA AATCCCTTGG CAGGTCATTT TAAATGGTGA AATTGAGGCA 
TTCTGTGGAG GTGCCATCAT TAATGAAAAA TGGATTGTAA CTGCTGCCCA CTGTCTTAAA 
CCTGGTGATA AAATTGAGGT TGTTGCTGGT GAATATAACA TTGATAAGAA GGAAGACACA 
GAACAAAGGA GAAATGTGAT TCGAACTATC CCTCATCACC AGTACAATGC AACTATTAAT 
AAGTATAGTC ATGACATTGC CTTGCTGGAA CTGGATAAAC CTTTAATACT AAACAGCTAT 
GTAACACCTA TCTGTGTTGC CAATAGGGAA TATACAAATA TCTTCCTCAA GTTTGGTTCT 
GGCTATGTCA GTGGCTGGGG AAAAGTCTTC AACAAAGGGA GACAGGCTTC CATTCTTCAG 
TACCTTAGAG TTCCACTGGT GGATAGAGCC ACATGCCTTA GGTCCACAAC ATTCACTATC 
TATAACAACA TGTTCTGTGC AGGCTACCGT GAAGGAGGCA AAGATTCGTG TGAAGGAGAT 
AGTGGGGGAC CCCATGTTAC TGAAGTAGAA GGGACAAGTT TCTTAACTGG CATTATTAGC 
TGGGGTGAAG AATGTGCAAT GAAAGGCAAA TATGGAATAT ATACTAAGGT TTCCCGGTAC 
GTCAACTGGA TTAAGGAAAA AACAAAGCTA ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032005.1
CDS	1..1416
Translation	MKHLNTVMAESPALITIFLLGYLLSTECAVFLDRENATKILTRPKRYNSGKLEEFVRGNLERECIEERCSFEEAREVFENTEKTTEFWKQYVDGDQCESNPCLNGGICKDDISSYECWCQVGFEGRNCELDATCNIKNGRCKQFCKNSPDNKVICSCTEGYQLAEDQKSCEPTVPFPCGRASISYSSKKITRAETVFSNMDYENSTEAVFIQDDITDGAILNNVTESSESLNDFTRVVGGENAKPGQIPWQVILNGEIEAFCGGAIINEKWIVTAAHCLKPGDKIEVVAGEYNIDKKEDTEQRRNVIRTIPHHQYNATINKYSHDIALLELDKPLILNSYVTPICVANREYTNIFLKFGSGYVSGWGKVFNKGRQASILQYLRVPLVDRATCLRSTTFTIYNNMFCAGYREGGKDSCEGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT

Target ORF information:

RefSeq Version	NM_007979.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus coagulation factor IX (F9), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007979.2

ORF Insert Sequence:

ATGAAGCACC TGAACACCGT CATGGCAGAA TCCCCGGCTC TCATCACCAT CTTCCTTTTA 
GGATATCTAC TCAGTACCGA ATGTGCAGTT TTCCTTGATC GTGAAAATGC CACCAAAATT 
CTTACCCGTC CAAAGAGATA TAATTCAGGA AAACTAGAAG AGTTTGTTCG AGGAAACCTT 
GAAAGAGAGT GTATAGAAGA AAGATGTAGT TTTGAAGAAG CACGAGAAGT TTTTGAAAAC 
ACTGAAAAAA CTACTGAATT TTGGAAGCAG TATGTTGATG GAGATCAGTG TGAATCAAAT 
CCTTGTTTAA ATGGTGGAAT ATGCAAGGAT GATATTAGTT CCTATGAATG CTGGTGCCAA 
GTTGGATTTG AAGGAAGGAA CTGTGAATTA GATGCAACGT GTAACATTAA AAATGGCAGG 
TGCAAGCAGT TTTGTAAAAA CAGTCCTGAT AACAAGGTAA TTTGTTCCTG CACTGAGGGA 
TACCAACTTG CAGAAGACCA GAAGTCCTGT GAACCAACAG TTCCATTTCC ATGTGGGAGA 
GCTTCTATTT CATACAGTTC TAAAAAGATC ACGAGAGCTG AGACTGTTTT CTCTAATATG 
GACTATGAAA ATTCTACTGA AGCTGTATTC ATTCAAGATG ACATCACTGA TGGTGCCATT 
CTTAATAACG TCACTGAAAG TAGTGAATCA CTTAATGACT TCACTCGAGT TGTTGGTGGA 
GAAAACGCAA AACCGGGTCA AATCCCTTGG CAGGTCATTT TAAATGGTGA AATTGAGGCA 
TTCTGTGGAG GTGCCATCAT TAATGAAAAA TGGATTGTAA CTGCTGCCCA CTGTCTTAAA 
CCTGGTGATA AAATTGAGGT TGTTGCTGGT GAATATAACA TTGATAAGAA GGAAGACACA 
GAACAAAGGA GAAATGTGAT TCGAACTATC CCTCATCACC AGTACAATGC AACTATTAAT 
AAGTATAGTC ATGACATTGC CTTGCTGGAA CTGGATAAAC CTTTAATACT AAACAGCTAT 
GTAACACCTA TCTGTGTTGC CAATAGGGAA TATACAAATA TCTTCCTCAA GTTTGGTTCT 
GGCTATGTCA GTGGCTGGGG AAAAGTCTTC AACAAAGGGA GACAGGCTTC CATTCTTCAG 
TACCTTAGAG TTCCACTGGT GGATAGAGCC ACATGCCTTA GGTCCACAAC ATTCACTATC 
TATAACAACA TGTTCTGTGC AGGCTACCGT GAAGGAGGCA AAGATTCGTG TGAAGGAGAT 
AGTGGGGGAC CCCATGTTAC TGAAGTAGAA GGGACAAGTT TCTTAACTGG CATTATTAGC 
TGGGGTGAAG AATGTGCAAT GAAAGGCAAA TATGGAATAT ATACTAAGGT TTCCCGGTAC 
GTCAACTGGA TTAAGGAAAA AACAAAGCTA ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F9 ( NM_001305797.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292726.1 Mus musculus coagulation factor IX (F9), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67704
Clone ID Related Accession (Same CDS sequence)	NM_001305797.1
Accession Version	NM_001305797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	coagulation factor IX isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK149372.1 and AA239875.1. Transcript Variant: This variant (2) lacks an alternate exon in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AI526776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCACC TGAACACCGT CATGGCAGAA TCCCCGGCTC TCATCACCAT CTTCCTTTTA 
GGATATCTAC TCAGTACCGA ATGTGCAGTT TTCCTTGATC GTGAAAATGC CACCAAAATT 
CTTACCCGTC CAAAGAGATA TAATTCAGGA AAACTAGAAG AGTTTGTTCG AGGAAACCTT 
GAAAGAGAGT GTATAGAAGA AAGATGTAGT TTTGAAGAAG CACGAGAAGT TTTTGAAAAC 
ACTGAAAAAA CTACTGAATT TTGGAAGCAG TATGTTGATG CAACGTGTAA CATTAAAAAT 
GGCAGGTGCA AGCAGTTTTG TAAAAACAGT CCTGATAACA AGGTAATTTG TTCCTGCACT 
GAGGGATACC AACTTGCAGA AGACCAGAAG TCCTGTGAAC CAACAGTTCC ATTTCCATGT 
GGGAGAGCTT CTATTTCATA CAGTTCTAAA AAGATCACGA GAGCTGAGAC TGTTTTCTCT 
AATATGGACT ATGAAAATTC TACTGAAGCT GTATTCATTC AAGATGACAT CACTGATGGT 
GCCATTCTTA ATAACGTCAC TGAAAGTAGT GAATCACTTA ATGACTTCAC TCGAGTTGTT 
GGTGGAGAAA ACGCAAAACC GGGTCAAATC CCTTGGCAGG TCATTTTAAA TGGTGAAATT 
GAGGCATTCT GTGGAGGTGC CATCATTAAT GAAAAATGGA TTGTAACTGC TGCCCACTGT 
CTTAAACCTG GTGATAAAAT TGAGGTTGTT GCTGGTGAAT ATAACATTGA TAAGAAGGAA 
GACACAGAAC AAAGGAGAAA TGTGATTCGA ACTATCCCTC ATCACCAGTA CAATGCAACT 
ATTAATAAGT ATAGTCATGA CATTGCCTTG CTGGAACTGG ATAAACCTTT AATACTAAAC 
AGCTATGTAA CACCTATCTG TGTTGCCAAT AGGGAATATA CAAATATCTT CCTCAAGTTT 
GGTTCTGGCT ATGTCAGTGG CTGGGGAAAA GTCTTCAACA AAGGGAGACA GGCTTCCATT 
CTTCAGTACC TTAGAGTTCC ACTGGTGGAT AGAGCCACAT GCCTTAGGTC CACAACATTC 
ACTATCTATA ACAACATGTT CTGTGCAGGC TACCGTGAAG GAGGCAAAGA TTCGTGTGAA 
GGAGATAGTG GGGGACCCCA TGTTACTGAA GTAGAAGGGA CAAGTTTCTT AACTGGCATT 
ATTAGCTGGG GTGAAGAATG TGCAATGAAA GGCAAATATG GAATATATAC TAAGGTTTCC 
CGGTACGTCA ACTGGATTAA GGAAAAAACA AAGCTAACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292726.1
CDS	1..1302
Translation	MKHLNTVMAESPALITIFLLGYLLSTECAVFLDRENATKILTRPKRYNSGKLEEFVRGNLERECIEERCSFEEAREVFENTEKTTEFWKQYVDATCNIKNGRCKQFCKNSPDNKVICSCTEGYQLAEDQKSCEPTVPFPCGRASISYSSKKITRAETVFSNMDYENSTEAVFIQDDITDGAILNNVTESSESLNDFTRVVGGENAKPGQIPWQVILNGEIEAFCGGAIINEKWIVTAAHCLKPGDKIEVVAGEYNIDKKEDTEQRRNVIRTIPHHQYNATINKYSHDIALLELDKPLILNSYVTPICVANREYTNIFLKFGSGYVSGWGKVFNKGRQASILQYLRVPLVDRATCLRSTTFTIYNNMFCAGYREGGKDSCEGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT

Target ORF information:

RefSeq Version	NM_001305797.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus coagulation factor IX (F9), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305797.1

ORF Insert Sequence:

ATGAAGCACC TGAACACCGT CATGGCAGAA TCCCCGGCTC TCATCACCAT CTTCCTTTTA 
GGATATCTAC TCAGTACCGA ATGTGCAGTT TTCCTTGATC GTGAAAATGC CACCAAAATT 
CTTACCCGTC CAAAGAGATA TAATTCAGGA AAACTAGAAG AGTTTGTTCG AGGAAACCTT 
GAAAGAGAGT GTATAGAAGA AAGATGTAGT TTTGAAGAAG CACGAGAAGT TTTTGAAAAC 
ACTGAAAAAA CTACTGAATT TTGGAAGCAG TATGTTGATG CAACGTGTAA CATTAAAAAT 
GGCAGGTGCA AGCAGTTTTG TAAAAACAGT CCTGATAACA AGGTAATTTG TTCCTGCACT 
GAGGGATACC AACTTGCAGA AGACCAGAAG TCCTGTGAAC CAACAGTTCC ATTTCCATGT 
GGGAGAGCTT CTATTTCATA CAGTTCTAAA AAGATCACGA GAGCTGAGAC TGTTTTCTCT 
AATATGGACT ATGAAAATTC TACTGAAGCT GTATTCATTC AAGATGACAT CACTGATGGT 
GCCATTCTTA ATAACGTCAC TGAAAGTAGT GAATCACTTA ATGACTTCAC TCGAGTTGTT 
GGTGGAGAAA ACGCAAAACC GGGTCAAATC CCTTGGCAGG TCATTTTAAA TGGTGAAATT 
GAGGCATTCT GTGGAGGTGC CATCATTAAT GAAAAATGGA TTGTAACTGC TGCCCACTGT 
CTTAAACCTG GTGATAAAAT TGAGGTTGTT GCTGGTGAAT ATAACATTGA TAAGAAGGAA 
GACACAGAAC AAAGGAGAAA TGTGATTCGA ACTATCCCTC ATCACCAGTA CAATGCAACT 
ATTAATAAGT ATAGTCATGA CATTGCCTTG CTGGAACTGG ATAAACCTTT AATACTAAAC 
AGCTATGTAA CACCTATCTG TGTTGCCAAT AGGGAATATA CAAATATCTT CCTCAAGTTT 
GGTTCTGGCT ATGTCAGTGG CTGGGGAAAA GTCTTCAACA AAGGGAGACA GGCTTCCATT 
CTTCAGTACC TTAGAGTTCC ACTGGTGGAT AGAGCCACAT GCCTTAGGTC CACAACATTC 
ACTATCTATA ACAACATGTT CTGTGCAGGC TACCGTGAAG GAGGCAAAGA TTCGTGTGAA 
GGAGATAGTG GGGGACCCCA TGTTACTGAA GTAGAAGGGA CAAGTTTCTT AACTGGCATT 
ATTAGCTGGG GTGAAGAATG TGCAATGAAA GGCAAATATG GAATATATAC TAAGGTTTCC 
CGGTACGTCA ACTGGATTAA GGAAAAAACA AAGCTAACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F9 ( XM_030251221.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107081.1 Mus musculus coagulation factor IX (F9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu90359
Clone ID Related Accession (Same CDS sequence)	XM_030251221.1
Accession Version	XM_030251221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	coagulation factor IX isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCACC TGAACACCGT CATGGCAGAA TCCCCGGCTC TCATCACCAT CTTCCTTTTA 
GGATATCTAC TCAGTACCGA ATGTGCAGTT TTCCTTGATC GTGAAAATGC CACCAAAATT 
CTTACCCGTC CAAAGAGATA TAATTCAGGA AAACTAGAAG AGTTTGTTCG AGGAAACCTT 
GAAAGAGAGT GTATAGAAGA AAGATGTAGT TTTGAAGAAG CACGAGAAGT TTTTGAAAAC 
ACTGAAAAAA CTACTGAATT TTGGAAGCAG TATGTTGATG GAGATCAGTG TGAATCAAAT 
CCTTGTTTAA ATGGTGGAAT ATGCAAGGAT GATATTAGTT CCTATGAATG CTGGTGCCAA 
GTTGGATTTG AAGGAAGGAA CTGTGAATTA GATGCAACGT GTAACATTAA AAATGGCAGG 
TGCAAGCAGT TTTGTAAAAA CAGTCCTGAT AACAAGGTAA TTTGTTCCTG CACTGAGGGA 
TACCAACTTG CAGAAGACCA GAAGTCCTGT GAACCAACAG GTCATTTTAA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107081.1
CDS	136..672
Translation	MKHLNTVMAESPALITIFLLGYLLSTECAVFLDRENATKILTRPKRYNSGKLEEFVRGNLERECIEERCSFEEAREVFENTEKTTEFWKQYVDGDQCESNPCLNGGICKDDISSYECWCQVGFEGRNCELDATCNIKNGRCKQFCKNSPDNKVICSCTEGYQLAEDQKSCEPTGHFKW

Target ORF information:

RefSeq Version	XM_030251221.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus coagulation factor IX (F9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251221.1

ORF Insert Sequence:

ATGAAGCACC TGAACACCGT CATGGCAGAA TCCCCGGCTC TCATCACCAT CTTCCTTTTA 
GGATATCTAC TCAGTACCGA ATGTGCAGTT TTCCTTGATC GTGAAAATGC CACCAAAATT 
CTTACCCGTC CAAAGAGATA TAATTCAGGA AAACTAGAAG AGTTTGTTCG AGGAAACCTT 
GAAAGAGAGT GTATAGAAGA AAGATGTAGT TTTGAAGAAG CACGAGAAGT TTTTGAAAAC 
ACTGAAAAAA CTACTGAATT TTGGAAGCAG TATGTTGATG GAGATCAGTG TGAATCAAAT 
CCTTGTTTAA ATGGTGGAAT ATGCAAGGAT GATATTAGTT CCTATGAATG CTGGTGCCAA 
GTTGGATTTG AAGGAAGGAA CTGTGAATTA GATGCAACGT GTAACATTAA AAATGGCAGG 
TGCAAGCAGT TTTGTAAAAA CAGTCCTGAT AACAAGGTAA TTTGTTCCTG CACTGAGGGA 
TACCAACTTG CAGAAGACCA GAAGTCCTGT GAACCAACAG GTCATTTTAA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Targeting anticoagulant protein S to improve hemostasis in hemophilia.
Blood(2018 Jan)
Prince R,Bologna L,Manetti M,Melchiorre D,Rosa I,Dewarrat N,Suardi S,Amini P,Fern?ndez JA,Burnier L,Quarroz C,Reina Caro MD,Matsumura Y,Kremer Hovinga JA,Griffin JH,Simon HU,Ibba-Manneschi L,Saller F,Calzavarini S,Angelillo-Scherrer A

VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo.
JCI insight3(1)(2018 Jan)
Lacombe J,Rishavy MA,Berkner KL,Ferron M

Genome editing for scalable production of alloantigen-free lentiviral vectors for
EMBO molecular medicine9(11)1558-1573(2017 Nov)
Milani M,Annoni A,Bartolaccini S,Biffi M,Russo F,Di Tomaso T,Raimondi A,Lengler J,Holmes MC,Scheiflinger F,Lombardo A,Cantore A,Naldini L

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F9 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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