F13b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	F13b
Entrez Gene ID	14060
Full Name	coagulation factor XIII, beta subunit
Synonyms	Cf-13b,Cf13b
General protein information	Preferred Names coagulation factor XIII, beta subunit Names coagulation factor XIII B chain protein-glutamine gamma-glutamyltransferase B chain transglutaminase B chain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 61.57 cM\|1 F
Summary	This gene encodes subunit B of the coagulation factor XIII that catalyzes the final step of the blood coagulation pathway. The encoded protein associates with subunit A to form a heterotetrameric protein that circulates in the plasma. During the blood coagulation process, thrombin-mediated proteolytic cleavage of the subunit A results in the dissociation of the encoded protein from the heterotetramer. Male mice lacking the encoded protein exhibit mild fibrosis together with hemosiderin deposits in the heart. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031164.2	NP_112441.2	coagulation factor XIII B chain precursor
XM_006529132.3	XP_006529195.1	coagulation factor XIII B chain isoform X1
XM_006529133.3	XP_006529196.1	coagulation factor XIII B chain isoform X2
XM_006529132.4	XP_006529195.1	coagulation factor XIII B chain isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	F13B	NP_001985.2
	F13B	XP_001111017.1
Canis lupus familiaris (dog)	F13B	XP_005622353.1
Pan troglodytes (chimpanzee)	F13B	XP_001137074.1
Bos taurus (cattle)	F13B	NP_001033618.1
Mus musculus (house mouse)	F13b	NP_112441.2
Gallus gallus (chicken)	F13B	XP_422198.4

Gene Ontology
Bibliography

Related articles in PubMed

The interaction between fibrinogen and zymogen FXIII-A2B2 is mediated by fibrinogen residues ?390-396 and the FXIII-B subunits.
Byrnes JR, Wilson C, Boutelle AM, Brandner CB, Flick MJ, Philippou H, Wolberg AS
Blood128(15)1969-1978(2016 10)

Transglutaminase 2 and Factor XIII catalyze distinct substrates in differentiating osteoblastic cell line: utility of highly reactive substrate peptides.
Watanabe K, Tsunoda K, Itoh M, Fukui M, Mori H, Hitomi K
Amino acids44(1)209-14(2013 Jan)

Male-specific cardiac pathologies in mice lacking either the A or B subunit of factor XIII.
Souri M, Koseki-Kuno S, Takeda N, Yamakawa M, Takeishi Y, Degen JL, Ichinose A
Thrombosis and haemostasis99(2)401-8(2008 Feb)

Administration of factor XIII B subunit increased plasma factor XIII A subunit levels in factor XIII B subunit knock-out mice.
Souri M, Koseki-Kuno S, Takeda N, Degen JL, Ichinose A
International journal of hematology87(1)60-8(2008 Jan)

Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides.
Bernhard OK, Kapp EA, Simpson RJ
Journal of proteome research6(3)987-95(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings provide insight into assembly of the fibrinogen/FXIII-A2B2 complex in both physiologic and therapeutic situations.
Title: The interaction between fibrinogen and zymogen FXIII-A2B2 is mediated by fibrinogen residues ?390-396 and the FXIII-B subunits.

Physiological significance of mouse TG2 and Factor XIII through identification of substrate proteins in MC3T3-E1 cells.
Title: Transglutaminase 2 and Factor XIII catalyze distinct substrates in differentiating osteoblastic cell line: utility of highly reactive substrate peptides.

Carditis and fibrosis in FXIII-deficient mice might be caused by a faulty or delayed reparative process that was initiated by abnormal haemorrhagic events within heart tissue.
Title: Male-specific cardiac pathologies in mice lacking either the A or B subunit of factor XIII.

The following F13b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F13b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu27899	XM_006529133.3 Latest version!	Mus musculus coagulation factor XIII, beta subunit (F13b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu12497	NM_031164.2 Latest version!	Mus musculus coagulation factor XIII, beta subunit (F13b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu27898	XM_006529132.4 Latest version!	Mus musculus coagulation factor XIII, beta subunit (F13b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu27898	XM_006529132.3	Mus musculus coagulation factor XIII, beta subunit (F13b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F13b ( XM_006529132.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529195.1 Mus musculus coagulation factor XIII, beta subunit (F13b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27898
Clone ID Related Accession (Same CDS sequence)	XM_006529132.3 , XM_006529132.4
Accession Version	XM_006529132.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	coagulation factor XIII B chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529132.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTAG ACAAAAAACT ATCATTCTTC TGTTTGGCTG GCTATGCAAC CGAAAGTGGG 
AAGCAAGAAG AGCAAATCAG GTGCACAGCA GAAGGCTGGT CTCCAAACCC AAGGTGCTAC 
AAGAAATGTC TGAAGCCTGA CCTAAGGAAT GGCTACGTCT CCAATGACAA AGTACTGTAC 
AAACTTCAAG AGAGGATGAG CTACGGTTGC TCTTCAGGAT ACAAAACCAC TGGGGGAAAG 
GACGAGGAGG TGGTCCACTG TCTTTCTGCA GGGTGGTCTT CTCAACCGTC CTGCAGGAAA 
GAACAAGAGA CATGTTTGGC CCCTGAGTTG GAACATGGAA ATTACTCCAC GACGCAGAGA 
ACATTCAAAG TGAAGGACAT AGTGGCTTAC ACATGCACTG CAGGCTACTA CACCACTACA 
GGGAAGCAGA CTGGGGAAGC AGAGTGCCAG GCTAATGGCT GGTCCCTCAC ACCACAGTGC 
AACAAATTAA TGTGCTCTTC TTTGAGATTA ATAGAAAATG GCTATTTTCA TCCTGTAAAG 
CAAACATATG AAGAAGGAGA TGTAGTTCAA TTTTTCTGTC ATGAAAATTA TTATCTAAGT 
GGATCTGATT TAATTCAGTG CTATAACTTT GGCTGGTATC CAGAATCTCC CATATGTGAA 
GGAAGAAGAA ATCGATGTCC TCCTCCACCT GTGCCTCTAA ATTCTAAAAT TCAACCACAT 
TCAACAACTT ACCGTCACGG AGAAAGAGTG CATATAGAAT GTGAACTTAA TTTTGTGATT 
CAGGGTTCAG AAGAACTGCT CTGTGAAAAT GGGAAGTGGA CAGAACCTCC AAAATGTATT 
GAGGAAAAGG AGAAGGTAGC CTGCGAGCAA CCACCCTCCG TTGAGAATGG TGTTGCACAC 
CCACACTCTG AGATTTACTA CAGTGGGGAT AAAGTGACAT ACAGGTGTGG AGGGGGCTAC 
AGTCTCAGAG GATCAAGTAC GATAACTTGC AATCGTGGCC GATGGACACT GCCCCCTGAG 
TGTGTTGAAA ATATTGAGAA TTGTAAGCCT CCTCCTGACA TAGCAAACGG TGTGGTTGTT 
GATGGGTTAC TGGCAAGTTA CACAACAGGA TCTTCTGTGG AGTACCGATG CAATGAGTAT 
TACTTATTGA AGGGGTCGGA AACATCTCGC TGTGAACAAG GAGCGTGGTC ATCTCCACCT 
GTCTGCTTGG AGCCCTGCAC TATTGATGTG GATCACATGA ACAGGAATAA CATACAGTTG 
AAGTGGAAAT ATGAGGGCAA GATTTTACAT GGAGACCTAA TAGATTTTGT GTGTAAGCAG 
GGATATAACT TGTCACCATC AATCCCACTG TCGGAGATAT CTGCGCAGTG CAATAGAGGC 
GATGTGAGGT ACCCCATGTG CATTAGAAAA GAATCTAAAG GGATGTGTGC ATCTCCTCCA 
GTGATAAGAA ATGGAGATAT TGTTAGTTCA GCAGCCCGCA CCTATGAAAA CGGCTCCTCA 
GTAGAATACC GGTGTTTTGA TAATCATTTC CTACAAGGGT CCCAGAATGT CTATTGTGTC 
GATGGAGTGT GGACTACACC ACCATCGTGT CTAGAGCCTT GCACATTATC ATTTGTTGAA 
ATGGATAAGA ATTATTTACA GCTGAAATGG AATTTTGACA ACAGACCACT CATTTTACAT 
GGAGAGTATA TTGAGTTTAT GTGTAAGAGA GATGCATATA TAAGTGAGAC ATCTATTGCT 
GGGTCTGTAC TTCGGGTGCA GTGTGACAGG GGAAGGCTGA AATACCCTAA GTGTACTCCA 
AGAGACAGGA GGCTGTCCTT TCAGGAAGCA TTACGGACCC GAAGACAAAT GGAAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529195.1
CDS	177..2039
Translation	MSVDKKLSFFCLAGYATESGKQEEQIRCTAEGWSPNPRCYKKCLKPDLRNGYVSNDKVLYKLQERMSYGCSSGYKTTGGKDEEVVHCLSAGWSSQPSCRKEQETCLAPELEHGNYSTTQRTFKVKDIVAYTCTAGYYTTTGKQTGEAECQANGWSLTPQCNKLMCSSLRLIENGYFHPVKQTYEEGDVVQFFCHENYYLSGSDLIQCYNFGWYPESPICEGRRNRCPPPPVPLNSKIQPHSTTYRHGERVHIECELNFVIQGSEELLCENGKWTEPPKCIEEKEKVACEQPPSVENGVAHPHSEIYYSGDKVTYRCGGGYSLRGSSTITCNRGRWTLPPECVENIENCKPPPDIANGVVVDGLLASYTTGSSVEYRCNEYYLLKGSETSRCEQGAWSSPPVCLEPCTIDVDHMNRNNIQLKWKYEGKILHGDLIDFVCKQGYNLSPSIPLSEISAQCNRGDVRYPMCIRKESKGMCASPPVIRNGDIVSSAARTYENGSSVEYRCFDNHFLQGSQNVYCVDGVWTTPPSCLEPCTLSFVEMDKNYLQLKWNFDNRPLILHGEYIEFMCKRDAYISETSIAGSVLRVQCDRGRLKYPKCTPRDRRLSFQEALRTRRQMEKR

Target ORF information:

RefSeq Version	XM_006529132.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus coagulation factor XIII, beta subunit (F13b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529132.3

ORF Insert Sequence:

ATGAGCGTAG ACAAAAAACT ATCATTCTTC TGTTTGGCTG GCTATGCAAC CGAAAGTGGG 
AAGCAAGAAG AGCAAATCAG GTGCACAGCA GAAGGCTGGT CTCCAAACCC AAGGTGCTAC 
AAGAAATGTC TGAAGCCTGA CCTAAGGAAT GGCTACGTCT CCAATGACAA AGTACTGTAC 
AAACTTCAAG AGAGGATGAG CTACGGTTGC TCTTCAGGAT ACAAAACCAC TGGGGGAAAG 
GACGAGGAGG TGGTCCACTG TCTTTCTGCA GGGTGGTCTT CTCAACCGTC CTGCAGGAAA 
GAACAAGAGA CATGTTTGGC CCCTGAGTTG GAACATGGAA ATTACTCCAC GACGCAGAGA 
ACATTCAAAG TGAAGGACAT AGTGGCTTAC ACATGCACTG CAGGCTACTA CACCACTACA 
GGGAAGCAGA CTGGGGAAGC AGAGTGCCAG GCTAATGGCT GGTCCCTCAC ACCACAGTGC 
AACAAATTAA TGTGCTCTTC TTTGAGATTA ATAGAAAATG GCTATTTTCA TCCTGTAAAG 
CAAACATATG AAGAAGGAGA TGTAGTTCAA TTTTTCTGTC ATGAAAATTA TTATCTAAGT 
GGATCTGATT TAATTCAGTG CTATAACTTT GGCTGGTATC CAGAATCTCC CATATGTGAA 
GGAAGAAGAA ATCGATGTCC TCCTCCACCT GTGCCTCTAA ATTCTAAAAT TCAACCACAT 
TCAACAACTT ACCGTCACGG AGAAAGAGTG CATATAGAAT GTGAACTTAA TTTTGTGATT 
CAGGGTTCAG AAGAACTGCT CTGTGAAAAT GGGAAGTGGA CAGAACCTCC AAAATGTATT 
GAGGAAAAGG AGAAGGTAGC CTGCGAGCAA CCACCCTCCG TTGAGAATGG TGTTGCACAC 
CCACACTCTG AGATTTACTA CAGTGGGGAT AAAGTGACAT ACAGGTGTGG AGGGGGCTAC 
AGTCTCAGAG GATCAAGTAC GATAACTTGC AATCGTGGCC GATGGACACT GCCCCCTGAG 
TGTGTTGAAA ATATTGAGAA TTGTAAGCCT CCTCCTGACA TAGCAAACGG TGTGGTTGTT 
GATGGGTTAC TGGCAAGTTA CACAACAGGA TCTTCTGTGG AGTACCGATG CAATGAGTAT 
TACTTATTGA AGGGGTCGGA AACATCTCGC TGTGAACAAG GAGCGTGGTC ATCTCCACCT 
GTCTGCTTGG AGCCCTGCAC TATTGATGTG GATCACATGA ACAGGAATAA CATACAGTTG 
AAGTGGAAAT ATGAGGGCAA GATTTTACAT GGAGACCTAA TAGATTTTGT GTGTAAGCAG 
GGATATAACT TGTCACCATC AATCCCACTG TCGGAGATAT CTGCGCAGTG CAATAGAGGC 
GATGTGAGGT ACCCCATGTG CATTAGAAAA GAATCTAAAG GGATGTGTGC ATCTCCTCCA 
GTGATAAGAA ATGGAGATAT TGTTAGTTCA GCAGCCCGCA CCTATGAAAA CGGCTCCTCA 
GTAGAATACC GGTGTTTTGA TAATCATTTC CTACAAGGGT CCCAGAATGT CTATTGTGTC 
GATGGAGTGT GGACTACACC ACCATCGTGT CTAGAGCCTT GCACATTATC ATTTGTTGAA 
ATGGATAAGA ATTATTTACA GCTGAAATGG AATTTTGACA ACAGACCACT CATTTTACAT 
GGAGAGTATA TTGAGTTTAT GTGTAAGAGA GATGCATATA TAAGTGAGAC ATCTATTGCT 
GGGTCTGTAC TTCGGGTGCA GTGTGACAGG GGAAGGCTGA AATACCCTAA GTGTACTCCA 
AGAGACAGGA GGCTGTCCTT TCAGGAAGCA TTACGGACCC GAAGACAAAT GGAAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F13b ( XM_006529133.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529196.1 Mus musculus coagulation factor XIII, beta subunit (F13b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu27899
Clone ID Related Accession (Same CDS sequence)	XM_006529133.3
Accession Version	XM_006529133.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	coagulation factor XIII B chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529133.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACGC TGAGACACTT GCCATTTATT CTTTTATTAA TCCTCTCAGG AGAACTCTAT 
GCAGAAGAGA AACAGTGTGA TTTTCCTACC GTGGAAAATG GAAGGATTGC CCAATATTAT 
TATACGTTTA AAAGCTTTTA CTTCCCCATG AGCGTAGACA AAAAACTATC ATTCTTCTGT 
TTGGCTGGCT ATGCAACCGA AAGTGGGAAG CAAGAAGAGC AAATCAGGTG CACAGCAGAA 
GGCTGGTCTC CAAACCCAAG GTGCTACAAG ACATGTTTGG CCCCTGAGTT GGAACATGGA 
AATTACTCCA CGACGCAGAG AACATTCAAA GTGAAGGACA TAGTGGCTTA CACATGCACT 
GCAGGCTACT ACACCACTAC AGGGAAGCAG ACTGGGGAAG CAGAGTGCCA GGCTAATGGC 
TGGTCCCTCA CACCACAGTG CAACAAATTA ATGTGCTCTT CTTTGAGATT AATAGAAAAT 
GGCTATTTTC ATCCTGTAAA GCAAACATAT GAAGAAGGAG ATGTAGTTCA ATTTTTCTGT 
CATGAAAATT ATTATCTAAG TGGATCTGAT TTAATTCAGT GCTATAACTT TGGCTGGTAT 
CCAGAATCTC CCATATGTGA AGGAAGAAGA AATCGATGTC CTCCTCCACC TGTGCCTCTA 
AATTCTAAAA TTCAACCACA TTCAACAACT TACCGTCACG GAGAAAGAGT GCATATAGAA 
TGTGAACTTA ATTTTGTGAT TCAGGGTTCA GAAGAACTGC TCTGTGAAAA TGGGAAGTGG 
ACAGAACCTC CAAAATGTAT TGAGGAAAAG GAGAAGGTAG CCTGCGAGCA ACCACCCTCC 
GTTGAGAATG GTGTTGCACA CCCACACTCT GAGATTTACT ACAGTGGGGA TAAAGTGACA 
TACAGGTGTG GAGGGGGCTA CAGTCTCAGA GGATCAAGTA CGATAACTTG CAATCGTGGC 
CGATGGACAC TGCCCCCTGA GTGTGTTGAA AATATTGAGA ATTGTAAGCC TCCTCCTGAC 
ATAGCAAACG GTGTGGTTGT TGATGGGTTA CTGGCAAGTT ACACAACAGG ATCTTCTGTG 
GAGTACCGAT GCAATGAGTA TTACTTATTG AAGGGGTCGG AAACATCTCG CTGTGAACAA 
GGAGCGTGGT CATCTCCACC TGTCTGCTTG GAGCCCTGCA CTATTGATGT GGATCACATG 
AACAGGAATA ACATACAGTT GAAGTGGAAA TATGAGGGCA AGATTTTACA TGGAGACCTA 
ATAGATTTTG TGTGTAAGCA GGGATATAAC TTGTCACCAT CAATCCCACT GTCGGAGATA 
TCTGCGCAGT GCAATAGAGG CGATGTGAGG TACCCCATGT GCATTAGAAA AGAATCTAAA 
GGGATGTGTG CATCTCCTCC AGTGATAAGA AATGGAGATA TTGTTAGTTC AGCAGCCCGC 
ACCTATGAAA ACGGCTCCTC AGTAGAATAC CGGTGTTTTG ATAATCATTT CCTACAAGGG 
TCCCAGAATG TCTATTGTGT CGATGGAGTG TGGACTACAC CACCATCGTG TCTAGAGCCT 
TGCACATTAT CATTTGTTGA AATGGATAAG AATTATTTAC AGCTGAAATG GAATTTTGAC 
AACAGACCAC TCATTTTACA TGGAGAGTAT ATTGAGTTTA TGTGTAAGAG AGATGCATAT 
ATAAGTGAGA CATCTATTGC TGGGTCTGTA CTTCGGGTGC AGTGTGACAG GGGAAGGCTG 
AAATACCCTA AGTGTACTCC AAGAGACAGG AGGCTGTCCT TTCAGGAAGC ATTACGGACC 
CGAAGACAAA TGGAAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529196.1
CDS	200..2023
Translation	MMTLRHLPFILLLILSGELYAEEKQCDFPTVENGRIAQYYYTFKSFYFPMSVDKKLSFFCLAGYATESGKQEEQIRCTAEGWSPNPRCYKTCLAPELEHGNYSTTQRTFKVKDIVAYTCTAGYYTTTGKQTGEAECQANGWSLTPQCNKLMCSSLRLIENGYFHPVKQTYEEGDVVQFFCHENYYLSGSDLIQCYNFGWYPESPICEGRRNRCPPPPVPLNSKIQPHSTTYRHGERVHIECELNFVIQGSEELLCENGKWTEPPKCIEEKEKVACEQPPSVENGVAHPHSEIYYSGDKVTYRCGGGYSLRGSSTITCNRGRWTLPPECVENIENCKPPPDIANGVVVDGLLASYTTGSSVEYRCNEYYLLKGSETSRCEQGAWSSPPVCLEPCTIDVDHMNRNNIQLKWKYEGKILHGDLIDFVCKQGYNLSPSIPLSEISAQCNRGDVRYPMCIRKESKGMCASPPVIRNGDIVSSAARTYENGSSVEYRCFDNHFLQGSQNVYCVDGVWTTPPSCLEPCTLSFVEMDKNYLQLKWNFDNRPLILHGEYIEFMCKRDAYISETSIAGSVLRVQCDRGRLKYPKCTPRDRRLSFQEALRTRRQMEKR

Target ORF information:

RefSeq Version	XM_006529133.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus coagulation factor XIII, beta subunit (F13b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529133.3

ORF Insert Sequence:

ATGATGACGC TGAGACACTT GCCATTTATT CTTTTATTAA TCCTCTCAGG AGAACTCTAT 
GCAGAAGAGA AACAGTGTGA TTTTCCTACC GTGGAAAATG GAAGGATTGC CCAATATTAT 
TATACGTTTA AAAGCTTTTA CTTCCCCATG AGCGTAGACA AAAAACTATC ATTCTTCTGT 
TTGGCTGGCT ATGCAACCGA AAGTGGGAAG CAAGAAGAGC AAATCAGGTG CACAGCAGAA 
GGCTGGTCTC CAAACCCAAG GTGCTACAAG ACATGTTTGG CCCCTGAGTT GGAACATGGA 
AATTACTCCA CGACGCAGAG AACATTCAAA GTGAAGGACA TAGTGGCTTA CACATGCACT 
GCAGGCTACT ACACCACTAC AGGGAAGCAG ACTGGGGAAG CAGAGTGCCA GGCTAATGGC 
TGGTCCCTCA CACCACAGTG CAACAAATTA ATGTGCTCTT CTTTGAGATT AATAGAAAAT 
GGCTATTTTC ATCCTGTAAA GCAAACATAT GAAGAAGGAG ATGTAGTTCA ATTTTTCTGT 
CATGAAAATT ATTATCTAAG TGGATCTGAT TTAATTCAGT GCTATAACTT TGGCTGGTAT 
CCAGAATCTC CCATATGTGA AGGAAGAAGA AATCGATGTC CTCCTCCACC TGTGCCTCTA 
AATTCTAAAA TTCAACCACA TTCAACAACT TACCGTCACG GAGAAAGAGT GCATATAGAA 
TGTGAACTTA ATTTTGTGAT TCAGGGTTCA GAAGAACTGC TCTGTGAAAA TGGGAAGTGG 
ACAGAACCTC CAAAATGTAT TGAGGAAAAG GAGAAGGTAG CCTGCGAGCA ACCACCCTCC 
GTTGAGAATG GTGTTGCACA CCCACACTCT GAGATTTACT ACAGTGGGGA TAAAGTGACA 
TACAGGTGTG GAGGGGGCTA CAGTCTCAGA GGATCAAGTA CGATAACTTG CAATCGTGGC 
CGATGGACAC TGCCCCCTGA GTGTGTTGAA AATATTGAGA ATTGTAAGCC TCCTCCTGAC 
ATAGCAAACG GTGTGGTTGT TGATGGGTTA CTGGCAAGTT ACACAACAGG ATCTTCTGTG 
GAGTACCGAT GCAATGAGTA TTACTTATTG AAGGGGTCGG AAACATCTCG CTGTGAACAA 
GGAGCGTGGT CATCTCCACC TGTCTGCTTG GAGCCCTGCA CTATTGATGT GGATCACATG 
AACAGGAATA ACATACAGTT GAAGTGGAAA TATGAGGGCA AGATTTTACA TGGAGACCTA 
ATAGATTTTG TGTGTAAGCA GGGATATAAC TTGTCACCAT CAATCCCACT GTCGGAGATA 
TCTGCGCAGT GCAATAGAGG CGATGTGAGG TACCCCATGT GCATTAGAAA AGAATCTAAA 
GGGATGTGTG CATCTCCTCC AGTGATAAGA AATGGAGATA TTGTTAGTTC AGCAGCCCGC 
ACCTATGAAA ACGGCTCCTC AGTAGAATAC CGGTGTTTTG ATAATCATTT CCTACAAGGG 
TCCCAGAATG TCTATTGTGT CGATGGAGTG TGGACTACAC CACCATCGTG TCTAGAGCCT 
TGCACATTAT CATTTGTTGA AATGGATAAG AATTATTTAC AGCTGAAATG GAATTTTGAC 
AACAGACCAC TCATTTTACA TGGAGAGTAT ATTGAGTTTA TGTGTAAGAG AGATGCATAT 
ATAAGTGAGA CATCTATTGC TGGGTCTGTA CTTCGGGTGC AGTGTGACAG GGGAAGGCTG 
AAATACCCTA AGTGTACTCC AAGAGACAGG AGGCTGTCCT TTCAGGAAGC ATTACGGACC 
CGAAGACAAA TGGAAAAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F13b ( NM_031164.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_112441.2 Mus musculus coagulation factor XIII, beta subunit (F13b), mRNA.

Price & Availability↑

CloneID	OMu12497
Clone ID Related Accession (Same CDS sequence)	NM_031164.2
Accession Version	NM_031164.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	coagulation factor XIII B chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW213546.1, AI527143.1, AK149397.1, AI115049.1 and BB718926.1. On Mar 13, 2009 this sequence version replaced NM_031164.1. ##Evidence-Data-START## Transcript exon combination :: D10071.1, AK149397.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN00849386 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGACGC TGAGACACTT GCCATTTATT CTTTTATTAA TCCTCTCAGG AGAACTCTAT 
GCAGAAGAGA AACAGTGTGA TTTTCCTACC GTGGAAAATG GAAGGATTGC CCAATATTAT 
TATACGTTTA AAAGCTTTTA CTTCCCCATG AGCGTAGACA AAAAACTATC ATTCTTCTGT 
TTGGCTGGCT ATGCAACCGA AAGTGGGAAG CAAGAAGAGC AAATCAGGTG CACAGCAGAA 
GGCTGGTCTC CAAACCCAAG GTGCTACAAG AAATGTCTGA AGCCTGACCT AAGGAATGGC 
TACGTCTCCA ATGACAAAGT ACTGTACAAA CTTCAAGAGA GGATGAGCTA CGGTTGCTCT 
TCAGGATACA AAACCACTGG GGGAAAGGAC GAGGAGGTGG TCCACTGTCT TTCTGCAGGG 
TGGTCTTCTC AACCGTCCTG CAGGAAAGAA CAAGAGACAT GTTTGGCCCC TGAGTTGGAA 
CATGGAAATT ACTCCACGAC GCAGAGAACA TTCAAAGTGA AGGACATAGT GGCTTACACA 
TGCACTGCAG GCTACTACAC CACTACAGGG AAGCAGACTG GGGAAGCAGA GTGCCAGGCT 
AATGGCTGGT CCCTCACACC ACAGTGCAAC AAATTAATGT GCTCTTCTTT GAGATTAATA 
GAAAATGGCT ATTTTCATCC TGTAAAGCAA ACATATGAAG AAGGAGATGT AGTTCAATTT 
TTCTGTCATG AAAATTATTA TCTAAGTGGA TCTGATTTAA TTCAGTGCTA TAACTTTGGC 
TGGTATCCAG AATCTCCCAT ATGTGAAGGA AGAAGAAATC GATGTCCTCC TCCACCTGTG 
CCTCTAAATT CTAAAATTCA ACCACATTCA ACAACTTACC GTCACGGAGA AAGAGTGCAT 
ATAGAATGTG AACTTAATTT TGTGATTCAG GGTTCAGAAG AACTGCTCTG TGAAAATGGG 
AAGTGGACAG AACCTCCAAA ATGTATTGAG GAAAAGGAGA AGGTAGCCTG CGAGCAACCA 
CCCTCCGTTG AGAATGGTGT TGCACACCCA CACTCTGAGA TTTACTACAG TGGGGATAAA 
GTGACATACA GGTGTGGAGG GGGCTACAGT CTCAGAGGAT CAAGTACGAT AACTTGCAAT 
CGTGGCCGAT GGACACTGCC CCCTGAGTGT GTTGAAAATA TTGAGAATTG TAAGCCTCCT 
CCTGACATAG CAAACGGTGT GGTTGTTGAT GGGTTACTGG CAAGTTACAC AACAGGATCT 
TCTGTGGAGT ACCGATGCAA TGAGTATTAC TTATTGAAGG GGTCGGAAAC ATCTCGCTGT 
GAACAAGGAG CGTGGTCATC TCCACCTGTC TGCTTGGAGC CCTGCACTAT TGATGTGGAT 
CACATGAACA GGAATAACAT ACAGTTGAAG TGGAAATATG AGGGCAAGAT TTTACATGGA 
GACCTAATAG ATTTTGTGTG TAAGCAGGGA TATAACTTGT CACCATCAAT CCCACTGTCG 
GAGATATCTG CGCAGTGCAA TAGAGGCGAT GTGAGGTACC CCATGTGCAT TAGAAAAGAA 
TCTAAAGGGA TGTGTGCATC TCCTCCAGTG ATAAGAAATG GAGATATTGT TAGTTCAGCA 
GCCCGCACCT ATGAAAACGG CTCCTCAGTA GAATACCGGT GTTTTGATAA TCATTTCCTA 
CAAGGGTCCC AGAATGTCTA TTGTGTCGAT GGAGTGTGGA CTACACCACC ATCGTGTCTA 
GAGCCTTGCA CATTATCATT TGTTGAAATG GATAAGAATT ATTTACAGCT GAAATGGAAT 
TTTGACAACA GACCACTCAT TTTACATGGA GAGTATATTG AGTTTATGTG TAAGAGAGAT 
GCATATATAA GTGAGACATC TATTGCTGGG TCTGTACTTC GGGTGCAGTG TGACAGGGGA 
AGGCTGAAAT ACCCTAAGTG TACTCCAAGA GACAGGAGGC TGTCCTTTCA GGAAGCATTA 
CGGACCCGAA GACAAATGGA AAAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112441.2
CDS	40..2049
Translation	MMTLRHLPFILLLILSGELYAEEKQCDFPTVENGRIAQYYYTFKSFYFPMSVDKKLSFFCLAGYATESGKQEEQIRCTAEGWSPNPRCYKKCLKPDLRNGYVSNDKVLYKLQERMSYGCSSGYKTTGGKDEEVVHCLSAGWSSQPSCRKEQETCLAPELEHGNYSTTQRTFKVKDIVAYTCTAGYYTTTGKQTGEAECQANGWSLTPQCNKLMCSSLRLIENGYFHPVKQTYEEGDVVQFFCHENYYLSGSDLIQCYNFGWYPESPICEGRRNRCPPPPVPLNSKIQPHSTTYRHGERVHIECELNFVIQGSEELLCENGKWTEPPKCIEEKEKVACEQPPSVENGVAHPHSEIYYSGDKVTYRCGGGYSLRGSSTITCNRGRWTLPPECVENIENCKPPPDIANGVVVDGLLASYTTGSSVEYRCNEYYLLKGSETSRCEQGAWSSPPVCLEPCTIDVDHMNRNNIQLKWKYEGKILHGDLIDFVCKQGYNLSPSIPLSEISAQCNRGDVRYPMCIRKESKGMCASPPVIRNGDIVSSAARTYENGSSVEYRCFDNHFLQGSQNVYCVDGVWTTPPSCLEPCTLSFVEMDKNYLQLKWNFDNRPLILHGEYIEFMCKRDAYISETSIAGSVLRVQCDRGRLKYPKCTPRDRRLSFQEALRTRRQMEKR

Target ORF information:

RefSeq Version	NM_031164.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus coagulation factor XIII, beta subunit (F13b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031164.2

ORF Insert Sequence:

ATGATGACGC TGAGACACTT GCCATTTATT CTTTTATTAA TCCTCTCAGG AGAACTCTAT 
GCAGAAGAGA AACAGTGTGA TTTTCCTACC GTGGAAAATG GAAGGATTGC CCAATATTAT 
TATACGTTTA AAAGCTTTTA CTTCCCCATG AGCGTAGACA AAAAACTATC ATTCTTCTGT 
TTGGCTGGCT ATGCAACCGA AAGTGGGAAG CAAGAAGAGC AAATCAGGTG CACAGCAGAA 
GGCTGGTCTC CAAACCCAAG GTGCTACAAG AAATGTCTGA AGCCTGACCT AAGGAATGGC 
TACGTCTCCA ATGACAAAGT ACTGTACAAA CTTCAAGAGA GGATGAGCTA CGGTTGCTCT 
TCAGGATACA AAACCACTGG GGGAAAGGAC GAGGAGGTGG TCCACTGTCT TTCTGCAGGG 
TGGTCTTCTC AACCGTCCTG CAGGAAAGAA CAAGAGACAT GTTTGGCCCC TGAGTTGGAA 
CATGGAAATT ACTCCACGAC GCAGAGAACA TTCAAAGTGA AGGACATAGT GGCTTACACA 
TGCACTGCAG GCTACTACAC CACTACAGGG AAGCAGACTG GGGAAGCAGA GTGCCAGGCT 
AATGGCTGGT CCCTCACACC ACAGTGCAAC AAATTAATGT GCTCTTCTTT GAGATTAATA 
GAAAATGGCT ATTTTCATCC TGTAAAGCAA ACATATGAAG AAGGAGATGT AGTTCAATTT 
TTCTGTCATG AAAATTATTA TCTAAGTGGA TCTGATTTAA TTCAGTGCTA TAACTTTGGC 
TGGTATCCAG AATCTCCCAT ATGTGAAGGA AGAAGAAATC GATGTCCTCC TCCACCTGTG 
CCTCTAAATT CTAAAATTCA ACCACATTCA ACAACTTACC GTCACGGAGA AAGAGTGCAT 
ATAGAATGTG AACTTAATTT TGTGATTCAG GGTTCAGAAG AACTGCTCTG TGAAAATGGG 
AAGTGGACAG AACCTCCAAA ATGTATTGAG GAAAAGGAGA AGGTAGCCTG CGAGCAACCA 
CCCTCCGTTG AGAATGGTGT TGCACACCCA CACTCTGAGA TTTACTACAG TGGGGATAAA 
GTGACATACA GGTGTGGAGG GGGCTACAGT CTCAGAGGAT CAAGTACGAT AACTTGCAAT 
CGTGGCCGAT GGACACTGCC CCCTGAGTGT GTTGAAAATA TTGAGAATTG TAAGCCTCCT 
CCTGACATAG CAAACGGTGT GGTTGTTGAT GGGTTACTGG CAAGTTACAC AACAGGATCT 
TCTGTGGAGT ACCGATGCAA TGAGTATTAC TTATTGAAGG GGTCGGAAAC ATCTCGCTGT 
GAACAAGGAG CGTGGTCATC TCCACCTGTC TGCTTGGAGC CCTGCACTAT TGATGTGGAT 
CACATGAACA GGAATAACAT ACAGTTGAAG TGGAAATATG AGGGCAAGAT TTTACATGGA 
GACCTAATAG ATTTTGTGTG TAAGCAGGGA TATAACTTGT CACCATCAAT CCCACTGTCG 
GAGATATCTG CGCAGTGCAA TAGAGGCGAT GTGAGGTACC CCATGTGCAT TAGAAAAGAA 
TCTAAAGGGA TGTGTGCATC TCCTCCAGTG ATAAGAAATG GAGATATTGT TAGTTCAGCA 
GCCCGCACCT ATGAAAACGG CTCCTCAGTA GAATACCGGT GTTTTGATAA TCATTTCCTA 
CAAGGGTCCC AGAATGTCTA TTGTGTCGAT GGAGTGTGGA CTACACCACC ATCGTGTCTA 
GAGCCTTGCA CATTATCATT TGTTGAAATG GATAAGAATT ATTTACAGCT GAAATGGAAT 
TTTGACAACA GACCACTCAT TTTACATGGA GAGTATATTG AGTTTATGTG TAAGAGAGAT 
GCATATATAA GTGAGACATC TATTGCTGGG TCTGTACTTC GGGTGCAGTG TGACAGGGGA 
AGGCTGAAAT ACCCTAAGTG TACTCCAAGA GACAGGAGGC TGTCCTTTCA GGAAGCATTA 
CGGACCCGAA GACAAATGGA AAAAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F13b ( XM_006529132.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529195.1 Mus musculus coagulation factor XIII, beta subunit (F13b), transcript variant X1, mRNA.

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CloneID	OMu27898
Clone ID Related Accession (Same CDS sequence)	XM_006529132.3 , XM_006529132.4
Accession Version	XM_006529132.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	coagulation factor XIII B chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529132.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTAG ACAAAAAACT ATCATTCTTC TGTTTGGCTG GCTATGCAAC CGAAAGTGGG 
AAGCAAGAAG AGCAAATCAG GTGCACAGCA GAAGGCTGGT CTCCAAACCC AAGGTGCTAC 
AAGAAATGTC TGAAGCCTGA CCTAAGGAAT GGCTACGTCT CCAATGACAA AGTACTGTAC 
AAACTTCAAG AGAGGATGAG CTACGGTTGC TCTTCAGGAT ACAAAACCAC TGGGGGAAAG 
GACGAGGAGG TGGTCCACTG TCTTTCTGCA GGGTGGTCTT CTCAACCGTC CTGCAGGAAA 
GAACAAGAGA CATGTTTGGC CCCTGAGTTG GAACATGGAA ATTACTCCAC GACGCAGAGA 
ACATTCAAAG TGAAGGACAT AGTGGCTTAC ACATGCACTG CAGGCTACTA CACCACTACA 
GGGAAGCAGA CTGGGGAAGC AGAGTGCCAG GCTAATGGCT GGTCCCTCAC ACCACAGTGC 
AACAAATTAA TGTGCTCTTC TTTGAGATTA ATAGAAAATG GCTATTTTCA TCCTGTAAAG 
CAAACATATG AAGAAGGAGA TGTAGTTCAA TTTTTCTGTC ATGAAAATTA TTATCTAAGT 
GGATCTGATT TAATTCAGTG CTATAACTTT GGCTGGTATC CAGAATCTCC CATATGTGAA 
GGAAGAAGAA ATCGATGTCC TCCTCCACCT GTGCCTCTAA ATTCTAAAAT TCAACCACAT 
TCAACAACTT ACCGTCACGG AGAAAGAGTG CATATAGAAT GTGAACTTAA TTTTGTGATT 
CAGGGTTCAG AAGAACTGCT CTGTGAAAAT GGGAAGTGGA CAGAACCTCC AAAATGTATT 
GAGGAAAAGG AGAAGGTAGC CTGCGAGCAA CCACCCTCCG TTGAGAATGG TGTTGCACAC 
CCACACTCTG AGATTTACTA CAGTGGGGAT AAAGTGACAT ACAGGTGTGG AGGGGGCTAC 
AGTCTCAGAG GATCAAGTAC GATAACTTGC AATCGTGGCC GATGGACACT GCCCCCTGAG 
TGTGTTGAAA ATATTGAGAA TTGTAAGCCT CCTCCTGACA TAGCAAACGG TGTGGTTGTT 
GATGGGTTAC TGGCAAGTTA CACAACAGGA TCTTCTGTGG AGTACCGATG CAATGAGTAT 
TACTTATTGA AGGGGTCGGA AACATCTCGC TGTGAACAAG GAGCGTGGTC ATCTCCACCT 
GTCTGCTTGG AGCCCTGCAC TATTGATGTG GATCACATGA ACAGGAATAA CATACAGTTG 
AAGTGGAAAT ATGAGGGCAA GATTTTACAT GGAGACCTAA TAGATTTTGT GTGTAAGCAG 
GGATATAACT TGTCACCATC AATCCCACTG TCGGAGATAT CTGCGCAGTG CAATAGAGGC 
GATGTGAGGT ACCCCATGTG CATTAGAAAA GAATCTAAAG GGATGTGTGC ATCTCCTCCA 
GTGATAAGAA ATGGAGATAT TGTTAGTTCA GCAGCCCGCA CCTATGAAAA CGGCTCCTCA 
GTAGAATACC GGTGTTTTGA TAATCATTTC CTACAAGGGT CCCAGAATGT CTATTGTGTC 
GATGGAGTGT GGACTACACC ACCATCGTGT CTAGAGCCTT GCACATTATC ATTTGTTGAA 
ATGGATAAGA ATTATTTACA GCTGAAATGG AATTTTGACA ACAGACCACT CATTTTACAT 
GGAGAGTATA TTGAGTTTAT GTGTAAGAGA GATGCATATA TAAGTGAGAC ATCTATTGCT 
GGGTCTGTAC TTCGGGTGCA GTGTGACAGG GGAAGGCTGA AATACCCTAA GTGTACTCCA 
AGAGACAGGA GGCTGTCCTT TCAGGAAGCA TTACGGACCC GAAGACAAAT GGAAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529195.1
CDS	359..2221
Translation	MSVDKKLSFFCLAGYATESGKQEEQIRCTAEGWSPNPRCYKKCLKPDLRNGYVSNDKVLYKLQERMSYGCSSGYKTTGGKDEEVVHCLSAGWSSQPSCRKEQETCLAPELEHGNYSTTQRTFKVKDIVAYTCTAGYYTTTGKQTGEAECQANGWSLTPQCNKLMCSSLRLIENGYFHPVKQTYEEGDVVQFFCHENYYLSGSDLIQCYNFGWYPESPICEGRRNRCPPPPVPLNSKIQPHSTTYRHGERVHIECELNFVIQGSEELLCENGKWTEPPKCIEEKEKVACEQPPSVENGVAHPHSEIYYSGDKVTYRCGGGYSLRGSSTITCNRGRWTLPPECVENIENCKPPPDIANGVVVDGLLASYTTGSSVEYRCNEYYLLKGSETSRCEQGAWSSPPVCLEPCTIDVDHMNRNNIQLKWKYEGKILHGDLIDFVCKQGYNLSPSIPLSEISAQCNRGDVRYPMCIRKESKGMCASPPVIRNGDIVSSAARTYENGSSVEYRCFDNHFLQGSQNVYCVDGVWTTPPSCLEPCTLSFVEMDKNYLQLKWNFDNRPLILHGEYIEFMCKRDAYISETSIAGSVLRVQCDRGRLKYPKCTPRDRRLSFQEALRTRRQMEKR

Target ORF information:

RefSeq Version	XM_006529132.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus coagulation factor XIII, beta subunit (F13b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529132.4

ORF Insert Sequence:

ATGAGCGTAG ACAAAAAACT ATCATTCTTC TGTTTGGCTG GCTATGCAAC CGAAAGTGGG 
AAGCAAGAAG AGCAAATCAG GTGCACAGCA GAAGGCTGGT CTCCAAACCC AAGGTGCTAC 
AAGAAATGTC TGAAGCCTGA CCTAAGGAAT GGCTACGTCT CCAATGACAA AGTACTGTAC 
AAACTTCAAG AGAGGATGAG CTACGGTTGC TCTTCAGGAT ACAAAACCAC TGGGGGAAAG 
GACGAGGAGG TGGTCCACTG TCTTTCTGCA GGGTGGTCTT CTCAACCGTC CTGCAGGAAA 
GAACAAGAGA CATGTTTGGC CCCTGAGTTG GAACATGGAA ATTACTCCAC GACGCAGAGA 
ACATTCAAAG TGAAGGACAT AGTGGCTTAC ACATGCACTG CAGGCTACTA CACCACTACA 
GGGAAGCAGA CTGGGGAAGC AGAGTGCCAG GCTAATGGCT GGTCCCTCAC ACCACAGTGC 
AACAAATTAA TGTGCTCTTC TTTGAGATTA ATAGAAAATG GCTATTTTCA TCCTGTAAAG 
CAAACATATG AAGAAGGAGA TGTAGTTCAA TTTTTCTGTC ATGAAAATTA TTATCTAAGT 
GGATCTGATT TAATTCAGTG CTATAACTTT GGCTGGTATC CAGAATCTCC CATATGTGAA 
GGAAGAAGAA ATCGATGTCC TCCTCCACCT GTGCCTCTAA ATTCTAAAAT TCAACCACAT 
TCAACAACTT ACCGTCACGG AGAAAGAGTG CATATAGAAT GTGAACTTAA TTTTGTGATT 
CAGGGTTCAG AAGAACTGCT CTGTGAAAAT GGGAAGTGGA CAGAACCTCC AAAATGTATT 
GAGGAAAAGG AGAAGGTAGC CTGCGAGCAA CCACCCTCCG TTGAGAATGG TGTTGCACAC 
CCACACTCTG AGATTTACTA CAGTGGGGAT AAAGTGACAT ACAGGTGTGG AGGGGGCTAC 
AGTCTCAGAG GATCAAGTAC GATAACTTGC AATCGTGGCC GATGGACACT GCCCCCTGAG 
TGTGTTGAAA ATATTGAGAA TTGTAAGCCT CCTCCTGACA TAGCAAACGG TGTGGTTGTT 
GATGGGTTAC TGGCAAGTTA CACAACAGGA TCTTCTGTGG AGTACCGATG CAATGAGTAT 
TACTTATTGA AGGGGTCGGA AACATCTCGC TGTGAACAAG GAGCGTGGTC ATCTCCACCT 
GTCTGCTTGG AGCCCTGCAC TATTGATGTG GATCACATGA ACAGGAATAA CATACAGTTG 
AAGTGGAAAT ATGAGGGCAA GATTTTACAT GGAGACCTAA TAGATTTTGT GTGTAAGCAG 
GGATATAACT TGTCACCATC AATCCCACTG TCGGAGATAT CTGCGCAGTG CAATAGAGGC 
GATGTGAGGT ACCCCATGTG CATTAGAAAA GAATCTAAAG GGATGTGTGC ATCTCCTCCA 
GTGATAAGAA ATGGAGATAT TGTTAGTTCA GCAGCCCGCA CCTATGAAAA CGGCTCCTCA 
GTAGAATACC GGTGTTTTGA TAATCATTTC CTACAAGGGT CCCAGAATGT CTATTGTGTC 
GATGGAGTGT GGACTACACC ACCATCGTGT CTAGAGCCTT GCACATTATC ATTTGTTGAA 
ATGGATAAGA ATTATTTACA GCTGAAATGG AATTTTGACA ACAGACCACT CATTTTACAT 
GGAGAGTATA TTGAGTTTAT GTGTAAGAGA GATGCATATA TAAGTGAGAC ATCTATTGCT 
GGGTCTGTAC TTCGGGTGCA GTGTGACAGG GGAAGGCTGA AATACCCTAA GTGTACTCCA 
AGAGACAGGA GGCTGTCCTT TCAGGAAGCA TTACGGACCC GAAGACAAAT GGAAAAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The interaction between fibrinogen and zymogen FXIII-A2B2 is mediated by fibrinogen residues ?390-396 and the FXIII-B subunits.
Blood128(15)1969-1978(2016 10)
Byrnes JR,Wilson C,Boutelle AM,Brandner CB,Flick MJ,Philippou H,Wolberg AS

Transglutaminase 2 and Factor XIII catalyze distinct substrates in differentiating osteoblastic cell line: utility of highly reactive substrate peptides.
Amino acids44(1)209-14(2013 Jan)
Watanabe K,Tsunoda K,Itoh M,Fukui M,Mori H,Hitomi K

Male-specific cardiac pathologies in mice lacking either the A or B subunit of factor XIII.
Thrombosis and haemostasis99(2)401-8(2008 Feb)
Souri M,Koseki-Kuno S,Takeda N,Yamakawa M,Takeishi Y,Degen JL,Ichinose A

Administration of factor XIII B subunit increased plasma factor XIII A subunit levels in factor XIII B subunit knock-out mice.
International journal of hematology87(1)60-8(2008 Jan)
Souri M,Koseki-Kuno S,Takeda N,Degen JL,Ichinose A

Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides.
Journal of proteome research6(3)987-95(2007 Mar)
Bernhard OK,Kapp EA,Simpson RJ

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F13b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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