Pofut1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pofut1
Entrez Gene ID	140484
Full Name	protein O-fucosyltransferase 1
Synonyms	O-FucT-1,mKIAA0180
General protein information	Preferred Names protein O-fucosyltransferase 1 Names GDP-fucose protein O-fucosyltransferase 1 peptide-O-fucosyltransferase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H1

mRNA and Protein(s)

mRNA	Protein	Name
XM_011239283.3	XP_011237585.1	GDP-fucose protein O-fucosyltransferase 1 isoform X1
NM_080463.3	NP_536711.3	GDP-fucose protein O-fucosyltransferase 1 precursor
XM_011239283.2	XP_011237585.1	GDP-fucose protein O-fucosyltransferase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	POFUT1	NP_056167.1
Pan troglodytes (chimpanzee)	POFUT1	NP_001008982.1
Danio rerio (zebrafish)	pofut1	NP_991281.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011338	XP_309312.4
Bos taurus (cattle)	POFUT1	NP_892031.1
Gallus gallus (chicken)	POFUT1	NP_989758.1
Drosophila melanogaster (fruit fly)	O-fut1	NP_610931.1
Caenorhabditis elegans (roundworm)	C15C7.7	NP_741744.2
Canis lupus familiaris (dog)	POFUT1	NP_001070825.1
Rattus norvegicus (Norway rat)	Pofut1	NP_001002278.1
Mus musculus (house mouse)	Pofut1	NP_536711.3
Xenopus tropicalis (tropical clawed frog)	pofut1	NP_001004774.1

Gene Ontology
Bibliography

Related articles in PubMed

Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1.
Wang Y, Wu B, Lu P, Zhang D, Wu B, Varshney S, Del Monte-Nieto G, Zhuang Z, Charafeddine R, Kramer AH, Sibinga NE, Frangogiannis NG, Kitsis RN, Adams RH, Alitalo K, Sharp DJ, Harvey RP, Stanley P, Zhou B
Nature communications8(1)578(2017 09)

Recognition of EGF-like domains by the Notch-modifying O-fucosyltransferase POFUT1.
Li Z, Han K, Pak JE, Satkunarajah M, Zhou D, Rini JM
Nature chemical biology13(7)757-763(2017 Jul)

Deletion of
Zygmunt DA, Singhal N, Kim ML, Cramer ML, Crowe KE, Xu R, Jia Y, Adair J, Martinez-Pena Y Valenzuela I, Akaaboune M, White P, Janssen PM, Martin PT
Molecular and cellular biology37(10)(2017 May)

Pofut1 point-mutations that disrupt O-fucosyltransferase activity destabilize the protein and abolish Notch1 signaling during mouse somitogenesis.
Ajima R, Suzuki E, Saga Y
PloS one12(11)e0187248(2017)

Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates.
Basch ML, Brown RM, Jen HI, Semerci F, Depreux F, Edlund RK, Zhang H, Norton CR, Gridley T, Cole SE, Doetzlhofer A, Maletic-Savatic M, Segil N, Groves AK
eLife5(2016 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

POFUT1 inactivation disrupts angiogenic signaling events and results in excessive angiogenic cell proliferation and plexus formation, leading to anomalous coronary arteries, myocardial infarction and heart failure.
Title: Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1.

The POFUT1 binds EGF-like domains of the hEGF type and that the highly correlated presence of POFUT1 and fucosylatable hEGFs has accompanied animal evolution.
Title: Recognition of EGF-like domains by the Notch-modifying O-fucosyltransferase POFUT1.

Loss of Pofut1 expression is associated with Muscle Aging-Related Phenotypes.
Title: Deletion of

both wild-type and mutant Pofut1 proteins were degraded through lysosome dependent machinery. Pofut1 protein loss in the point mutant embryos caused the same phenotypes as those observed in Pofut1 null embryos
Title: Pofut1 point-mutations that disrupt O-fucosyltransferase activity destabilize the protein and abolish Notch1 signaling during mouse somitogenesis.

results demonstrated that POFUT1-mediated O-fucosylation of NOTCH receptors regulates myogenic cell differentiation and affects postnatal muscle growth in mice
Title: Reduced Notch signalling leads to postnatal skeletal muscle hypertrophy in Pofut1cax/cax mice.

The following Pofut1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pofut1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09682	NM_080463.3 Latest version!	Mus musculus protein O-fucosyltransferase 1 (Pofut1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu72460	XM_011239283.3 Latest version!	Mus musculus protein O-fucosyltransferase 1 (Pofut1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu72460	XM_011239283.2	Mus musculus protein O-fucosyltransferase 1 (Pofut1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pofut1 ( XM_011239283.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237585.1 Mus musculus protein O-fucosyltransferase 1 (Pofut1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu72460
Clone ID Related Accession (Same CDS sequence)	XM_011239283.3 , XM_011239283.2
Accession Version	XM_011239283.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GDP-fucose protein O-fucosyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATC TGGCACCCTC CCACTGGCCC CCTGAGAAGC GAGTGGCATA CTGCTTTGAG 
GTGGCAGCCC AGCGAAGTCC TGATAAGAAG ACATGTCCCA TGAAGGAAGG AAATCCTTTT 
GGGCCATTCT GGGACCAGTT TCATGTGAGT TTCAATAAGT CAGAACTGTT CACAGGCATT 
TCCTTCAGCG CCTCCTACAA AGAACAATGG ACCCAGAGAT TTCCTGCAAA AGAGCATCCT 
GTGCTCGCAC TGCCTGGGGC CCCAGCACAG TTCCCTGTCC TGGAGGAACA CAGGGAGCTC 
CAGAAGTACA TGGTGTGGTC AGATGAGATG GTGAGGACGG GAGAGGCCCT GATCAGTGCC 
CACCTCGTCC GGCCCTATGT GGGCATTCAT CTGCGCATTG GCTCCGACTG GAAGAATGCC 
TGTGCCATGC TGAAGGATGG AACTGCAGGG TCACACTTCA TGGCTTCCCC TCAGTGTGTG 
GGCTATAGCC GCAGCACAGC GACCCCTCTC ACCATGACCA TGTGCCTCCC TGACCTGAAG 
GAAATCCAGC GGGCTGTGAC GCTTTGGGTG AGAGCACTGA ATGCCAGATC GGTCTACATC 
GCCACAGACT CTGAGAGCTA CGTGTCAGAG ATCCAGCAGC TCTTCAAAGA CAAGGTGAGG 
GTGGTGAGCC TGAAACCCGA GGTGGCCCAG ATCGACCTGT ACATCCTCGG CCAGGCTGAC 
CACTTCATTG GAAACTGTGT CTCCTCGTTC ACTGCCTTCG TGAAGCGGGA GCGGGACCTC 
CATGGGAGGC AGTCGTCCTT CTTTGGCATG GACAGACCCT CCCAGCTTCG GGATGAATTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237585.1
CDS	233..1075
Translation	MENLAPSHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYKEQWTQRFPAKEHPVLALPGAPAQFPVLEEHRELQKYMVWSDEMVRTGEALISAHLVRPYVGIHLRIGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTATPLTMTMCLPDLKEIQRAVTLWVRALNARSVYIATDSESYVSEIQQLFKDKVRVVSLKPEVAQIDLYILGQADHFIGNCVSSFTAFVKRERDLHGRQSSFFGMDRPSQLRDEF

Target ORF information:

RefSeq Version	XM_011239283.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein O-fucosyltransferase 1 (Pofut1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239283.2

ORF Insert Sequence:

ATGGAAAATC TGGCACCCTC CCACTGGCCC CCTGAGAAGC GAGTGGCATA CTGCTTTGAG 
GTGGCAGCCC AGCGAAGTCC TGATAAGAAG ACATGTCCCA TGAAGGAAGG AAATCCTTTT 
GGGCCATTCT GGGACCAGTT TCATGTGAGT TTCAATAAGT CAGAACTGTT CACAGGCATT 
TCCTTCAGCG CCTCCTACAA AGAACAATGG ACCCAGAGAT TTCCTGCAAA AGAGCATCCT 
GTGCTCGCAC TGCCTGGGGC CCCAGCACAG TTCCCTGTCC TGGAGGAACA CAGGGAGCTC 
CAGAAGTACA TGGTGTGGTC AGATGAGATG GTGAGGACGG GAGAGGCCCT GATCAGTGCC 
CACCTCGTCC GGCCCTATGT GGGCATTCAT CTGCGCATTG GCTCCGACTG GAAGAATGCC 
TGTGCCATGC TGAAGGATGG AACTGCAGGG TCACACTTCA TGGCTTCCCC TCAGTGTGTG 
GGCTATAGCC GCAGCACAGC GACCCCTCTC ACCATGACCA TGTGCCTCCC TGACCTGAAG 
GAAATCCAGC GGGCTGTGAC GCTTTGGGTG AGAGCACTGA ATGCCAGATC GGTCTACATC 
GCCACAGACT CTGAGAGCTA CGTGTCAGAG ATCCAGCAGC TCTTCAAAGA CAAGGTGAGG 
GTGGTGAGCC TGAAACCCGA GGTGGCCCAG ATCGACCTGT ACATCCTCGG CCAGGCTGAC 
CACTTCATTG GAAACTGTGT CTCCTCGTTC ACTGCCTTCG TGAAGCGGGA GCGGGACCTC 
CATGGGAGGC AGTCGTCCTT CTTTGGCATG GACAGACCCT CCCAGCTTCG GGATGAATTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pofut1 ( NM_080463.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_536711.3 Mus musculus protein O-fucosyltransferase 1 (Pofut1), mRNA.

Price & Availability↑

CloneID	OMu09682
Clone ID Related Accession (Same CDS sequence)	NM_080463.3
Accession Version	NM_080463.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	GDP-fucose protein O-fucosyltransferase 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB853248.1, AK132301.1 and CB521087.1. On Jan 12, 2006 this sequence version replaced NM_080463.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132301.1, AK081059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGCCG CCGCGTGGGC ACCGCCACAC CTGCTGCTGC GGGCGTCTTT CCTGCTTCTG 
CTGCTGTTGC TGCCGCTCCG CGGGCGGTCA GCGGGCTCCT GGGACCTGGC CGGTTACCTG 
CTCTACTGTC CCTGCATGGG GCGCTTTGGG AACCAGGCTG ATCACTTCTT GGGCTCCCTG 
GCATTTGCGA AGCTGCTGAA CCGCACCTTG GCTGTACCTC CATGGATTGA ATACCAACAT 
CACAAGCCTC CTTTCACCAA CCTCCATGTG TCCTACCAAA AGTACTTCAA ACTGGAGCCT 
CTCCAAGCCT ACCATCGGGT TGTCAGCCTG GAGGACTTCA TGGAAAATCT GGCACCCTCC 
CACTGGCCCC CTGAGAAGCG AGTGGCATAC TGCTTTGAGG TGGCAGCCCA GCGAAGTCCT 
GATAAGAAGA CATGTCCCAT GAAGGAAGGA AATCCTTTTG GGCCATTCTG GGACCAGTTT 
CATGTGAGTT TCAATAAGTC AGAACTGTTC ACAGGCATTT CCTTCAGCGC CTCCTACAAA 
GAACAATGGA CCCAGAGATT TCCTGCAAAA GAGCATCCTG TGCTCGCACT GCCTGGGGCC 
CCAGCACAGT TCCCTGTCCT GGAGGAACAC AGGGAGCTCC AGAAGTACAT GGTGTGGTCA 
GATGAGATGG TGAGGACGGG AGAGGCCCTG ATCAGTGCCC ACCTCGTCCG GCCCTATGTG 
GGCATTCATC TGCGCATTGG CTCCGACTGG AAGAATGCCT GTGCCATGCT GAAGGATGGA 
ACTGCAGGGT CACACTTCAT GGCTTCCCCT CAGTGTGTGG GCTATAGCCG CAGCACAGCG 
ACCCCTCTCA CCATGACCAT GTGCCTCCCT GACCTGAAGG AAATCCAGCG GGCTGTGACG 
CTTTGGGTGA GAGCACTGAA TGCCAGATCG GTCTACATCG CCACAGACTC TGAGAGCTAC 
GTGTCAGAGA TCCAGCAGCT CTTCAAAGAC AAGGTGAGGG TGGTGAGCCT GAAACCCGAG 
GTGGCCCAGA TCGACCTGTA CATCCTCGGC CAGGCTGACC ACTTCATTGG AAACTGTGTC 
TCCTCGTTCA CTGCCTTCGT GAAGCGGGAG CGGGACCTCC ATGGGAGGCA GTCGTCCTTC 
TTTGGCATGG ACAGACCCTC CCAGCTTCGG GATGAATTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536711.3
CDS	56..1237
Translation	MGAAAWAPPHLLLRASFLLLLLLLPLRGRSAGSWDLAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVVSLEDFMENLAPSHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYKEQWTQRFPAKEHPVLALPGAPAQFPVLEEHRELQKYMVWSDEMVRTGEALISAHLVRPYVGIHLRIGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTATPLTMTMCLPDLKEIQRAVTLWVRALNARSVYIATDSESYVSEIQQLFKDKVRVVSLKPEVAQIDLYILGQADHFIGNCVSSFTAFVKRERDLHGRQSSFFGMDRPSQLRDEF

Target ORF information:

RefSeq Version	NM_080463.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein O-fucosyltransferase 1 (Pofut1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080463.3

ORF Insert Sequence:

ATGGGCGCCG CCGCGTGGGC ACCGCCACAC CTGCTGCTGC GGGCGTCTTT CCTGCTTCTG 
CTGCTGTTGC TGCCGCTCCG CGGGCGGTCA GCGGGCTCCT GGGACCTGGC CGGTTACCTG 
CTCTACTGTC CCTGCATGGG GCGCTTTGGG AACCAGGCTG ATCACTTCTT GGGCTCCCTG 
GCATTTGCGA AGCTGCTGAA CCGCACCTTG GCTGTACCTC CATGGATTGA ATACCAACAT 
CACAAGCCTC CTTTCACCAA CCTCCATGTG TCCTACCAAA AGTACTTCAA ACTGGAGCCT 
CTCCAAGCCT ACCATCGGGT TGTCAGCCTG GAGGACTTCA TGGAAAATCT GGCACCCTCC 
CACTGGCCCC CTGAGAAGCG AGTGGCATAC TGCTTTGAGG TGGCAGCCCA GCGAAGTCCT 
GATAAGAAGA CATGTCCCAT GAAGGAAGGA AATCCTTTTG GGCCATTCTG GGACCAGTTT 
CATGTGAGTT TCAATAAGTC AGAACTGTTC ACAGGCATTT CCTTCAGCGC CTCCTACAAA 
GAACAATGGA CCCAGAGATT TCCTGCAAAA GAGCATCCTG TGCTCGCACT GCCTGGGGCC 
CCAGCACAGT TCCCTGTCCT GGAGGAACAC AGGGAGCTCC AGAAGTACAT GGTGTGGTCA 
GATGAGATGG TGAGGACGGG AGAGGCCCTG ATCAGTGCCC ACCTCGTCCG GCCCTATGTG 
GGCATTCATC TGCGCATTGG CTCCGACTGG AAGAATGCCT GTGCCATGCT GAAGGATGGA 
ACTGCAGGGT CACACTTCAT GGCTTCCCCT CAGTGTGTGG GCTATAGCCG CAGCACAGCG 
ACCCCTCTCA CCATGACCAT GTGCCTCCCT GACCTGAAGG AAATCCAGCG GGCTGTGACG 
CTTTGGGTGA GAGCACTGAA TGCCAGATCG GTCTACATCG CCACAGACTC TGAGAGCTAC 
GTGTCAGAGA TCCAGCAGCT CTTCAAAGAC AAGGTGAGGG TGGTGAGCCT GAAACCCGAG 
GTGGCCCAGA TCGACCTGTA CATCCTCGGC CAGGCTGACC ACTTCATTGG AAACTGTGTC 
TCCTCGTTCA CTGCCTTCGT GAAGCGGGAG CGGGACCTCC ATGGGAGGCA GTCGTCCTTC 
TTTGGCATGG ACAGACCCTC CCAGCTTCGG GATGAATTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pofut1 ( XM_011239283.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237585.1 Mus musculus protein O-fucosyltransferase 1 (Pofut1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu72460
Clone ID Related Accession (Same CDS sequence)	XM_011239283.3 , XM_011239283.2
Accession Version	XM_011239283.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GDP-fucose protein O-fucosyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239283.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATC TGGCACCCTC CCACTGGCCC CCTGAGAAGC GAGTGGCATA CTGCTTTGAG 
GTGGCAGCCC AGCGAAGTCC TGATAAGAAG ACATGTCCCA TGAAGGAAGG AAATCCTTTT 
GGGCCATTCT GGGACCAGTT TCATGTGAGT TTCAATAAGT CAGAACTGTT CACAGGCATT 
TCCTTCAGCG CCTCCTACAA AGAACAATGG ACCCAGAGAT TTCCTGCAAA AGAGCATCCT 
GTGCTCGCAC TGCCTGGGGC CCCAGCACAG TTCCCTGTCC TGGAGGAACA CAGGGAGCTC 
CAGAAGTACA TGGTGTGGTC AGATGAGATG GTGAGGACGG GAGAGGCCCT GATCAGTGCC 
CACCTCGTCC GGCCCTATGT GGGCATTCAT CTGCGCATTG GCTCCGACTG GAAGAATGCC 
TGTGCCATGC TGAAGGATGG AACTGCAGGG TCACACTTCA TGGCTTCCCC TCAGTGTGTG 
GGCTATAGCC GCAGCACAGC GACCCCTCTC ACCATGACCA TGTGCCTCCC TGACCTGAAG 
GAAATCCAGC GGGCTGTGAC GCTTTGGGTG AGAGCACTGA ATGCCAGATC GGTCTACATC 
GCCACAGACT CTGAGAGCTA CGTGTCAGAG ATCCAGCAGC TCTTCAAAGA CAAGGTGAGG 
GTGGTGAGCC TGAAACCCGA GGTGGCCCAG ATCGACCTGT ACATCCTCGG CCAGGCTGAC 
CACTTCATTG GAAACTGTGT CTCCTCGTTC ACTGCCTTCG TGAAGCGGGA GCGGGACCTC 
CATGGGAGGC AGTCGTCCTT CTTTGGCATG GACAGACCCT CCCAGCTTCG GGATGAATTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237585.1
CDS	236..1078
Translation	MENLAPSHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYKEQWTQRFPAKEHPVLALPGAPAQFPVLEEHRELQKYMVWSDEMVRTGEALISAHLVRPYVGIHLRIGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTATPLTMTMCLPDLKEIQRAVTLWVRALNARSVYIATDSESYVSEIQQLFKDKVRVVSLKPEVAQIDLYILGQADHFIGNCVSSFTAFVKRERDLHGRQSSFFGMDRPSQLRDEF

Target ORF information:

RefSeq Version	XM_011239283.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein O-fucosyltransferase 1 (Pofut1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239283.3

ORF Insert Sequence:

ATGGAAAATC TGGCACCCTC CCACTGGCCC CCTGAGAAGC GAGTGGCATA CTGCTTTGAG 
GTGGCAGCCC AGCGAAGTCC TGATAAGAAG ACATGTCCCA TGAAGGAAGG AAATCCTTTT 
GGGCCATTCT GGGACCAGTT TCATGTGAGT TTCAATAAGT CAGAACTGTT CACAGGCATT 
TCCTTCAGCG CCTCCTACAA AGAACAATGG ACCCAGAGAT TTCCTGCAAA AGAGCATCCT 
GTGCTCGCAC TGCCTGGGGC CCCAGCACAG TTCCCTGTCC TGGAGGAACA CAGGGAGCTC 
CAGAAGTACA TGGTGTGGTC AGATGAGATG GTGAGGACGG GAGAGGCCCT GATCAGTGCC 
CACCTCGTCC GGCCCTATGT GGGCATTCAT CTGCGCATTG GCTCCGACTG GAAGAATGCC 
TGTGCCATGC TGAAGGATGG AACTGCAGGG TCACACTTCA TGGCTTCCCC TCAGTGTGTG 
GGCTATAGCC GCAGCACAGC GACCCCTCTC ACCATGACCA TGTGCCTCCC TGACCTGAAG 
GAAATCCAGC GGGCTGTGAC GCTTTGGGTG AGAGCACTGA ATGCCAGATC GGTCTACATC 
GCCACAGACT CTGAGAGCTA CGTGTCAGAG ATCCAGCAGC TCTTCAAAGA CAAGGTGAGG 
GTGGTGAGCC TGAAACCCGA GGTGGCCCAG ATCGACCTGT ACATCCTCGG CCAGGCTGAC 
CACTTCATTG GAAACTGTGT CTCCTCGTTC ACTGCCTTCG TGAAGCGGGA GCGGGACCTC 
CATGGGAGGC AGTCGTCCTT CTTTGGCATG GACAGACCCT CCCAGCTTCG GGATGAATTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1.
Nature communications8(1)578(2017 09)
Wang Y,Wu B,Lu P,Zhang D,Wu B,Varshney S,Del Monte-Nieto G,Zhuang Z,Charafeddine R,Kramer AH,Sibinga NE,Frangogiannis NG,Kitsis RN,Adams RH,Alitalo K,Sharp DJ,Harvey RP,Stanley P,Zhou B

Recognition of EGF-like domains by the Notch-modifying O-fucosyltransferase POFUT1.
Nature chemical biology13(7)757-763(2017 Jul)
Li Z,Han K,Pak JE,Satkunarajah M,Zhou D,Rini JM

Deletion of
Molecular and cellular biology37(10)(2017 May)
Zygmunt DA,Singhal N,Kim ML,Cramer ML,Crowe KE,Xu R,Jia Y,Adair J,Martinez-Pena Y Valenzuela I,Akaaboune M,White P,Janssen PM,Martin PT

Pofut1 point-mutations that disrupt O-fucosyltransferase activity destabilize the protein and abolish Notch1 signaling during mouse somitogenesis.
PloS one12(11)e0187248(2017)
Ajima R,Suzuki E,Saga Y

Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates.
eLife5(2016 12)
Basch ML,Brown RM,Jen HI,Semerci F,Depreux F,Edlund RK,Zhang H,Norton CR,Gridley T,Cole SE,Doetzlhofer A,Maletic-Savatic M,Segil N,Groves AK

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Pofut1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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