Ext1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ext1
Entrez Gene ID	14042
Full Name	exostoses (multiple) 1
Synonyms	AA409028
General protein information	Preferred Names exostoses (multiple) 1 Names exostosin-1 glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase multiple exostoses protein 1 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 C\|15 20.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010162.2	NP_034292.2	exostosin-1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ext1b	NP_001012369.2
Xenopus tropicalis (tropical clawed frog)	ext1	NP_001006730.1
Homo sapiens (human)	EXT1	NP_000118.2
	EXT1	XP_001096808.1
Gallus gallus (chicken)	LOC100858205	XP_003640902.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010388	XP_311558.3
Canis lupus familiaris (dog)	EXT1	XP_539145.2
Bos taurus (cattle)	EXT1	NP_001091564.1
Mus musculus (house mouse)	Ext1	NP_034292.2
Rattus norvegicus (Norway rat)	Ext1	NP_001124012.1
Caenorhabditis elegans (roundworm)	rib-1	NP_502180.1
Pan troglodytes (chimpanzee)	EXT1	XP_001141496.1
Drosophila melanogaster (fruit fly)	ttv	NP_477231.1

Gene Ontology
Bibliography

Related articles in PubMed

Epithelial heparan sulfate regulates Sonic Hedgehog signaling in lung development.
He H, Huang M, Sun S, Wu Y, Lin X
PLoS genetics13(8)e1006992(2017 Aug)

Unsuspected osteochondroma-like outgrowths in the cranial base of Hereditary Multiple Exostoses patients and modeling and treatment with a BMP antagonist in mice.
Sinha S, Mundy C, Bechtold T, Sgariglia F, Ibrahim MM, Billings PC, Carroll K, Koyama E, Jones KB, Pacifici M
PLoS genetics13(4)e1006742(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

reactivating SHH signaling in mutant lungs rescued the tip dilation phenotype and attenuated FGF signaling. Importantly, the reduced SHH signaling activity did not appear to be caused by decreased Shh expression or protein stability; instead, biologically active form of SHH proteins were reduced in both the Ext1 mutant epithelium and surrounding wild type mesenchymal cells.
Title: Epithelial heparan sulfate regulates Sonic Hedgehog signaling in lung development.

Normal heparin sulfate levels and Ext1 activity in the stromal microenvironment and hematopoietic stem cells are required for the transplant engraftment following non-myeloablative conditioning.
Title: Effect of systemic heparan sulfate haploinsufficiency on steady state hematopoiesis and engraftment of hematopoietic stem cells.

crucial for outflow tract formation in distinct progenitor cells and modulates FGF signaling during early heart development
Title: Heparan Sulfate Biosynthesis Enzyme, Ext1, Contributes to Outflow Tract Development of Mouse Heart via Modulation of FGF Signaling.

Heterozygous loss of function of EXT1 and EXT2 results in a decreased arteriolar endothelial glycocalyx but improved flow mediated vasodilation.
Title: Loss of function in heparan sulfate elongation genes EXT1 and EXT 2 results in improved nitric oxide bioavailability and endothelial function.

Ext genes and heparan sulfate are needed to establish and maintain perichondrium's phenotype and border function.
Title: Perichondrium phenotype and border function are regulated by Ext1 and heparan sulfate in developing long bones: a mechanism likely deranged in Hereditary Multiple Exostoses.

The following Ext1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ext1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22346	NM_010162.2 Latest version!	Mus musculus exostosin glycosyltransferase 1 (Ext1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ext1 ( NM_010162.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034292.2 Mus musculus exostosin glycosyltransferase 1 (Ext1), mRNA.

Price & Availability↑

CloneID	OMu22346
Clone ID Related Accession (Same CDS sequence)	NM_010162.2
Accession Version	NM_010162.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	exostosin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ045320.1, AK132327.1 and BI133786.1. On Aug 19, 2006 this sequence version replaced NM_010162.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132327.1, X96639.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGCCA AAAAACGCTA TTTCATCCTG CTCTCAGCTG GCTCTTGTCT CGCCCTTTTG 
TTTTATTTTG GAGGCGTGCA GTTTAGGGCA TCGAGGAGCC ACAGCCGGAG AGAAGAGCAC 
AGTGGTCGGA ATGGCTTGCA CCAGCCCAGT CCGGATCATT TCTGGCCCCG CTTCCCGGAC 
GCTCTGCGCC CTTTCTTTCC TTGGGATCAA TTGGAAAACG AGGATTCCAG CGTGCACATT 
TCCCCCCGGC AGAAGCGAGA CGCCAACTCG AGCATCTACA AAGGCAAGAA GTGCCGCATG 
GAGTCCTGCT TCGATTTCAC CCTTTGCAAG AAAAACGGCT TCAAAGTCTA CGTGTACCCG 
CAGCAGAAAG GGGAGAAAAT CGCCGAAAGT TACCAAAACA TTCTAGCGGC CATCGAGGGC 
TCCAGGTTCT ACACCTCGGA CCCCAGCCAG GCGTGCCTCT TTGTCTTGAG TCTGGATACT 
TTAGACAGAG ACCAGTTATC ACCTCAGTAT GTGCACAATT TGAGATCCAA AGTGCAGAGT 
CTCCACTTGT GGAACAATGG TAGGAATCAT TTAATTTTTA ATTTATATTC TGGCACTTGG 
CCTGACTACA CTGAGGACGT GGGGTTTGAC ATCGGCCAGG CGATGCTGGC CAAAGCCAGC 
ATCAGTACTG AAAACTTCCG ACCAAACTTT GATGTTTCTA TTCCCCTCTT TTCTAAGGAT 
CATCCCAGGA CAGGAGGGGA GAGGGGGTTT TTGAAATTTA ACACCATCCC TCCTCTCAGG 
AAGTACATGC TGGTATTCAA GGGGAAGCGG TACCTGACAG GGATAGGGTC AGACACCAGG 
AATGCCTTAT ATCACGTCCA TAACGGGGAG GACGTCTTGC TCCTCACCAC CTGCAAGCAT 
GGCAAAGACT GGCAAAAGCA CAAGGATTCT CGCTGTGACA GAGACAACAC CGAGTATGAG 
AAATATGATT ATCGGGAAAT GCTGCACAAT GCCACTTTCT GTCTGGTTCC TCGTGGTCGC 
AGGCTTGGGT CCTTCAGATT CCTGGAGGCT TTGCAGGCTG CCTGTGTCCC TGTAATGCTC 
AGCAACGGAT GGGAGTTGCC ATTCTCCGAA GTGATTAATT GGAACCAAGC TGCCGTCATA 
GGCGATGAGA GATTGCTATT ACAGATTCCT TCTACAATCA GGTCTATTCA TCAGGATAAA 
ATCCTAGCAC TTAGACAGCA GACACAGTTC TTGTGGGAGG CTTATTTTTC TTCAGTTGAG 
AAGATTGTAT TAACTACACT AGAGATTATT CAGGACAGAA TATTCAAGCA CATATCACGT 
AACAGTTTAA TATGGAACAA ACATCCTGGA GGATTGTTCG TCCTACCGCA GTATTCATCT 
TACCTGGGAG ATTTCCCTTA CTACTATGCT AATTTAGGTT TAAAGCCCCC CTCCAAATTC 
ACTGCAGTCA TCCATGCTGT GACTCCCCTG GTCTCTCAGT CCCAGCCAGT GTTGAAGCTT 
CTTGTGGCTG CAGCCAAATC CCAGTACTGT GCGCAGATCA TAGTTCTGTG GAATTGTGAC 
AAGCCTCTAC CAGCCAAACA TCGCTGGCCT GCCACTGCCG TGCCTGTCAT CGTCATTGAA 
GGAGAAAGCA AGGTTATGAG CAGCCGGTTT CTGCCCTATG ACAACATCAT CACTGATGCT 
GTGCTCAGCC TGGATGAGGA CACTGTGCTT TCAACTACGG AAGTGGATTT TGCCTTCACC 
GTATGGCAGA GCTTCCCAGA GAGGATTGTG GGATATCCTG CTCGCAGTCA TTTCTGGGAT 
AACTCAAAGG AGCGGTGGGG ATATACATCC AAGTGGACGA ATGACTACTC CATGGTGTTG 
ACAGGAGCTG CTATCTACCA CAAATATTAT CACTACCTGT ATTCCCATTA CCTGCCAGCC 
AGCCTGAAGA ACATGGTAGA CCAACTGGCC AACTGTGAGG ACATTCTCAT GAATTTCCTG 
GTGTCTGCTG TGACAAAATT GCCTCCAATC AAAGTGACCC AGAAGAAACA GTATAAGGAG 
ACAATGATGG GACAGACTTC CCGAGCATCC CGCTGGGCCG ACCCTGACCA CTTTGCCCAG 
CGACAGAGCT GCATGAATAC ATTTGCCAGC TGGTTTGGCT ACATGCCGCT GATCCATTCT 
CAGATGAGGC TGGACCCGGT CCTCTTTAAA GACCAAGTCT CAATTCTGAG GAAGAAATAC 
AGAGACATTG AACGACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034292.2
CDS	821..3061
Translation	MQAKKRYFILLSAGSCLALLFYFGGVQFRASRSHSRREEHSGRNGLHQPSPDHFWPRFPDALRPFFPWDQLENEDSSVHISPRQKRDANSSIYKGKKCRMESCFDFTLCKKNGFKVYVYPQQKGEKIAESYQNILAAIEGSRFYTSDPSQACLFVLSLDTLDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLIFNLYSGTWPDYTEDVGFDIGQAMLAKASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVFKGKRYLTGIGSDTRNALYHVHNGEDVLLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFCLVPRGRRLGSFRFLEALQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPSTIRSIHQDKILALRQQTQFLWEAYFSSVEKIVLTTLEIIQDRIFKHISRNSLIWNKHPGGLFVLPQYSSYLGDFPYYYANLGLKPPSKFTAVIHAVTPLVSQSQPVLKLLVAAAKSQYCAQIIVLWNCDKPLPAKHRWPATAVPVIVIEGESKVMSSRFLPYDNIITDAVLSLDEDTVLSTTEVDFAFTVWQSFPERIVGYPARSHFWDNSKERWGYTSKWTNDYSMVLTGAAIYHKYYHYLYSHYLPASLKNMVDQLANCEDILMNFLVSAVTKLPPIKVTQKKQYKETMMGQTSRASRWADPDHFAQRQSCMNTFASWFGYMPLIHSQMRLDPVLFKDQVSILRKKYRDIERL

Target ORF information:

RefSeq Version	NM_010162.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus exostosin glycosyltransferase 1 (Ext1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010162.2

ORF Insert Sequence:

ATGCAGGCCA AAAAACGCTA TTTCATCCTG CTCTCAGCTG GCTCTTGTCT CGCCCTTTTG 
TTTTATTTTG GAGGCGTGCA GTTTAGGGCA TCGAGGAGCC ACAGCCGGAG AGAAGAGCAC 
AGTGGTCGGA ATGGCTTGCA CCAGCCCAGT CCGGATCATT TCTGGCCCCG CTTCCCGGAC 
GCTCTGCGCC CTTTCTTTCC TTGGGATCAA TTGGAAAACG AGGATTCCAG CGTGCACATT 
TCCCCCCGGC AGAAGCGAGA CGCCAACTCG AGCATCTACA AAGGCAAGAA GTGCCGCATG 
GAGTCCTGCT TCGATTTCAC CCTTTGCAAG AAAAACGGCT TCAAAGTCTA CGTGTACCCG 
CAGCAGAAAG GGGAGAAAAT CGCCGAAAGT TACCAAAACA TTCTAGCGGC CATCGAGGGC 
TCCAGGTTCT ACACCTCGGA CCCCAGCCAG GCGTGCCTCT TTGTCTTGAG TCTGGATACT 
TTAGACAGAG ACCAGTTATC ACCTCAGTAT GTGCACAATT TGAGATCCAA AGTGCAGAGT 
CTCCACTTGT GGAACAATGG TAGGAATCAT TTAATTTTTA ATTTATATTC TGGCACTTGG 
CCTGACTACA CTGAGGACGT GGGGTTTGAC ATCGGCCAGG CGATGCTGGC CAAAGCCAGC 
ATCAGTACTG AAAACTTCCG ACCAAACTTT GATGTTTCTA TTCCCCTCTT TTCTAAGGAT 
CATCCCAGGA CAGGAGGGGA GAGGGGGTTT TTGAAATTTA ACACCATCCC TCCTCTCAGG 
AAGTACATGC TGGTATTCAA GGGGAAGCGG TACCTGACAG GGATAGGGTC AGACACCAGG 
AATGCCTTAT ATCACGTCCA TAACGGGGAG GACGTCTTGC TCCTCACCAC CTGCAAGCAT 
GGCAAAGACT GGCAAAAGCA CAAGGATTCT CGCTGTGACA GAGACAACAC CGAGTATGAG 
AAATATGATT ATCGGGAAAT GCTGCACAAT GCCACTTTCT GTCTGGTTCC TCGTGGTCGC 
AGGCTTGGGT CCTTCAGATT CCTGGAGGCT TTGCAGGCTG CCTGTGTCCC TGTAATGCTC 
AGCAACGGAT GGGAGTTGCC ATTCTCCGAA GTGATTAATT GGAACCAAGC TGCCGTCATA 
GGCGATGAGA GATTGCTATT ACAGATTCCT TCTACAATCA GGTCTATTCA TCAGGATAAA 
ATCCTAGCAC TTAGACAGCA GACACAGTTC TTGTGGGAGG CTTATTTTTC TTCAGTTGAG 
AAGATTGTAT TAACTACACT AGAGATTATT CAGGACAGAA TATTCAAGCA CATATCACGT 
AACAGTTTAA TATGGAACAA ACATCCTGGA GGATTGTTCG TCCTACCGCA GTATTCATCT 
TACCTGGGAG ATTTCCCTTA CTACTATGCT AATTTAGGTT TAAAGCCCCC CTCCAAATTC 
ACTGCAGTCA TCCATGCTGT GACTCCCCTG GTCTCTCAGT CCCAGCCAGT GTTGAAGCTT 
CTTGTGGCTG CAGCCAAATC CCAGTACTGT GCGCAGATCA TAGTTCTGTG GAATTGTGAC 
AAGCCTCTAC CAGCCAAACA TCGCTGGCCT GCCACTGCCG TGCCTGTCAT CGTCATTGAA 
GGAGAAAGCA AGGTTATGAG CAGCCGGTTT CTGCCCTATG ACAACATCAT CACTGATGCT 
GTGCTCAGCC TGGATGAGGA CACTGTGCTT TCAACTACGG AAGTGGATTT TGCCTTCACC 
GTATGGCAGA GCTTCCCAGA GAGGATTGTG GGATATCCTG CTCGCAGTCA TTTCTGGGAT 
AACTCAAAGG AGCGGTGGGG ATATACATCC AAGTGGACGA ATGACTACTC CATGGTGTTG 
ACAGGAGCTG CTATCTACCA CAAATATTAT CACTACCTGT ATTCCCATTA CCTGCCAGCC 
AGCCTGAAGA ACATGGTAGA CCAACTGGCC AACTGTGAGG ACATTCTCAT GAATTTCCTG 
GTGTCTGCTG TGACAAAATT GCCTCCAATC AAAGTGACCC AGAAGAAACA GTATAAGGAG 
ACAATGATGG GACAGACTTC CCGAGCATCC CGCTGGGCCG ACCCTGACCA CTTTGCCCAG 
CGACAGAGCT GCATGAATAC ATTTGCCAGC TGGTTTGGCT ACATGCCGCT GATCCATTCT 
CAGATGAGGC TGGACCCGGT CCTCTTTAAA GACCAAGTCT CAATTCTGAG GAAGAAATAC 
AGAGACATTG AACGACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Epithelial heparan sulfate regulates Sonic Hedgehog signaling in lung development.
PLoS genetics13(8)e1006992(2017 Aug)
He H,Huang M,Sun S,Wu Y,Lin X

Unsuspected osteochondroma-like outgrowths in the cranial base of Hereditary Multiple Exostoses patients and modeling and treatment with a BMP antagonist in mice.
PLoS genetics13(4)e1006742(2017 Apr)
Sinha S,Mundy C,Bechtold T,Sgariglia F,Ibrahim MM,Billings PC,Carroll K,Koyama E,Jones KB,Pacifici M

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Ext1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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