Epm2a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Epm2a
Entrez Gene ID	13853
Full Name	epilepsy, progressive myoclonic epilepsy, type 2 gene alpha
Synonyms	TG-B,Tg(TcraK,TcrbK)TG-BFlv
General protein information	Preferred Names epilepsy, progressive myoclonic epilepsy, type 2 gene alpha Names laforin LAFPTPase glucan phosphatase lafora PTPase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_010146.2	NP_034276.2	laforin

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	EPM2A	NP_001026240.1
Mus musculus (house mouse)	Epm2a	NP_034276.2
Danio rerio (zebrafish)	epm2a	XP_003201207.2
Bos taurus (cattle)	EPM2A	NP_001093179.1
	EPM2A	XP_001087462.1
Canis lupus familiaris (dog)	EPM2A	XP_005615541.1
Rattus norvegicus (Norway rat)	Epm2a	NP_001263691.1
Homo sapiens (human)	EPM2A	NP_005661.1
Pan troglodytes (chimpanzee)	EPM2A	XP_001161292.2
Xenopus tropicalis (tropical clawed frog)	epm2a	NP_001123695.1

Gene Ontology
Bibliography

Related articles in PubMed

Suppression of leptin signaling reduces polyglucosan inclusions and seizure susceptibility in a mouse model for Lafora disease.
Rai A, Mishra R, Ganesh S
Human molecular genetics26(24)4778-4785(2017 Dec)

Abnormal glycogen chain length pattern, not hyperphosphorylation, is critical in Lafora disease.
Nitschke F, Sullivan MA, Wang P, Zhao X, Chown EE, Perri AM, Israelian L, Juana-L?pez L, Bovolenta P, Rodr?guez de C?rdoba S, Steup M, Minassian BA
EMBO molecular medicine9(7)906-917(2017 Jul)

Lafora Disease Is an Inherited Metabolic Cardiomyopathy.
Villalba-Orero M, S?nchez-Elexpuru G, L?pez-Ola?eta M, Campuzano O, Bello-Arroyo E, Garc?a-Pav?a P, Serratosa JM, Brugada R, S?nchez MP, Lara-Pezzi E
Journal of the American College of Cardiology69(24)3007-3009(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Laforin-silenced cells was able to induce autophagic flux, proteasomal activity and reduce the polyubiquitinated proteins by heat shock.
Title: Loss of malin, but not laforin, results in compromised autophagic flux and proteasomal dysfunction in cells exposed to heat shock.

This study also suggests a malin function independent of laforin, possibly in lysosomal biogenesis and/or lysosomal glycogen disposal.
Title: Protein degradation and quality control in cells from laforin and malin knockout mice.

expression of Epm2a blocks formation of Lafora bodies and restores the impairment in macroautophagy, preventing the development of Lafora bodies in Epm2a-deficient mice.
Title: The phosphatase activity of laforin is dispensable to rescue Epm2a-/- mice from Lafora disease.

loss of laforin results in activation of serum/glucocorticoid-induced kinase 1 in cellular and animals models
Title: Activation of serum/glucocorticoid-induced kinase 1 (SGK1) underlies increased glycogen levels, mTOR activation, and autophagy defects in Lafora disease.

in laforin-deficient mice, stress drastically accelerates Lafora bodies accumulation and Lafora disease.
Title: Laforin prevents stress-induced polyglucosan body formation and Lafora disease progression in neurons.

The following Epm2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Epm2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18607	NM_010146.2 Latest version!	Mus musculus epilepsy, progressive myoclonic epilepsy, type 2 gene alpha (Epm2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Epm2a ( NM_010146.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034276.2 Mus musculus epilepsy, progressive myoclonic epilepsy, type 2 gene alpha (Epm2a), mRNA.

Price & Availability↑

CloneID	OMu18607
Clone ID Related Accession (Same CDS sequence)	NM_010146.2
Accession Version	NM_010146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	laforin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY730431.1, CN532498.1 and AC101984.13. On Oct 12, 2006 this sequence version replaced NM_010146.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF124044.1, SRR1660813.23658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTCTTCC GCTTCGGCGT GGTGGTGCCG CCCGCGGTGG CCGGCGCTCG GCAGGAGCTG 
CTGCTCGCCG GCTCGCGGCC CGAGTTAGGG CGCTGGGAGC CGCACGGCGC CGTGCGTCTC 
AGGCCCGCGG GTACCGCGGC GGGCGCCGCT GCGCTGGCGC TGCAGGAGCC CGGCCTGTGG 
CTCGCCGAGG TGGAGCTGGA GGCGTACGAG GAGGCCGGCG GGGCGGAGCC GGGCCGCGTT 
GACACGTTCT GGTACAAGTT CCTGCAGCGC GAGCCTGGAG GCGAGCTGCA CTGGGAAGGA 
AATGGACCTC ACCATGACCG TTGCTGCACA TATAATGAGG ACAACTTGGT GGATGGTGTT 
TATTGTCTCC CAGTAGGACA CTGGATTGAG GCCACTGGGC ACACCAATGA AATGAAGCAC 
ACAACAGACT TCTATTTTAA TATTGCTGGC CACCAAGCCA TGCACTATTC AAGAATTCTA 
CCAAATATCT GGCTGGGTAG CTGCCCTCGC CAACTGGAAC ATGTGACCAT CAAACTGAAG 
CATGAACTGG GAGTTACAGC TGTCATGAAT TTCCAGACTG AATGGGATAT CATCCAGAAT 
TCTTCAGGCT GCAACCGCTA CCCTGAACCC ATGACTCCAG ACACCATGAT GAAGCTGTAT 
AAGGAAGAAG GCTTGTCCTA CATCTGGATG CCCACTCCAG ACATGAGCAC TGAGGGCCGA 
GTGCAGATGC TGCCACAGGC TGTGTGTCTC CTGCACGCGC TTCTGGAGAA TGGACACACG 
GTGTATGTCC ACTGCAACGC TGGCGTGGGT CGCTCCACAG CTGCAGTGTG CGGCTGGCTC 
CACTATGTGA TTGGCTGGAA TCTGCGCAAG GTGCAGTACT TCATCATGGC CAAGAGGCCT 
GCGGTCTACA TTGACGAGGA CGCTTTGGCT CAAGCACAAC AAGACTTTTC TCAGAAGTTC 
GGGAAGGTTC ACTCTTCCAT ATGCGCTTTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034276.2
CDS	27..1019
Translation	MLFRFGVVVPPAVAGARQELLLAGSRPELGRWEPHGAVRLRPAGTAAGAAALALQEPGLWLAEVELEAYEEAGGAEPGRVDTFWYKFLQREPGGELHWEGNGPHHDRCCTYNEDNLVDGVYCLPVGHWIEATGHTNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCPRQLEHVTIKLKHELGVTAVMNFQTEWDIIQNSSGCNRYPEPMTPDTMMKLYKEEGLSYIWMPTPDMSTEGRVQMLPQAVCLLHALLENGHTVYVHCNAGVGRSTAAVCGWLHYVIGWNLRKVQYFIMAKRPAVYIDEDALAQAQQDFSQKFGKVHSSICAL

Target ORF information:

RefSeq Version	NM_010146.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus epilepsy, progressive myoclonic epilepsy, type 2 gene alpha (Epm2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010146.2

ORF Insert Sequence:

ATGCTCTTCC GCTTCGGCGT GGTGGTGCCG CCCGCGGTGG CCGGCGCTCG GCAGGAGCTG 
CTGCTCGCCG GCTCGCGGCC CGAGTTAGGG CGCTGGGAGC CGCACGGCGC CGTGCGTCTC 
AGGCCCGCGG GTACCGCGGC GGGCGCCGCT GCGCTGGCGC TGCAGGAGCC CGGCCTGTGG 
CTCGCCGAGG TGGAGCTGGA GGCGTACGAG GAGGCCGGCG GGGCGGAGCC GGGCCGCGTT 
GACACGTTCT GGTACAAGTT CCTGCAGCGC GAGCCTGGAG GCGAGCTGCA CTGGGAAGGA 
AATGGACCTC ACCATGACCG TTGCTGCACA TATAATGAGG ACAACTTGGT GGATGGTGTT 
TATTGTCTCC CAGTAGGACA CTGGATTGAG GCCACTGGGC ACACCAATGA AATGAAGCAC 
ACAACAGACT TCTATTTTAA TATTGCTGGC CACCAAGCCA TGCACTATTC AAGAATTCTA 
CCAAATATCT GGCTGGGTAG CTGCCCTCGC CAACTGGAAC ATGTGACCAT CAAACTGAAG 
CATGAACTGG GAGTTACAGC TGTCATGAAT TTCCAGACTG AATGGGATAT CATCCAGAAT 
TCTTCAGGCT GCAACCGCTA CCCTGAACCC ATGACTCCAG ACACCATGAT GAAGCTGTAT 
AAGGAAGAAG GCTTGTCCTA CATCTGGATG CCCACTCCAG ACATGAGCAC TGAGGGCCGA 
GTGCAGATGC TGCCACAGGC TGTGTGTCTC CTGCACGCGC TTCTGGAGAA TGGACACACG 
GTGTATGTCC ACTGCAACGC TGGCGTGGGT CGCTCCACAG CTGCAGTGTG CGGCTGGCTC 
CACTATGTGA TTGGCTGGAA TCTGCGCAAG GTGCAGTACT TCATCATGGC CAAGAGGCCT 
GCGGTCTACA TTGACGAGGA CGCTTTGGCT CAAGCACAAC AAGACTTTTC TCAGAAGTTC 
GGGAAGGTTC ACTCTTCCAT ATGCGCTTTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Suppression of leptin signaling reduces polyglucosan inclusions and seizure susceptibility in a mouse model for Lafora disease.
Human molecular genetics26(24)4778-4785(2017 Dec)
Rai A,Mishra R,Ganesh S

Abnormal glycogen chain length pattern, not hyperphosphorylation, is critical in Lafora disease.
EMBO molecular medicine9(7)906-917(2017 Jul)
Nitschke F,Sullivan MA,Wang P,Zhao X,Chown EE,Perri AM,Israelian L,Juana-L?pez L,Bovolenta P,Rodr?guez de C?rdoba S,Steup M,Minassian BA

Lafora Disease Is an Inherited Metabolic Cardiomyopathy.
Journal of the American College of Cardiology69(24)3007-3009(2017 Jun)
Villalba-Orero M,S?nchez-Elexpuru G,L?pez-Ola?eta M,Campuzano O,Bello-Arroyo E,Garc?a-Pav?a P,Serratosa JM,Brugada R,S?nchez MP,Lara-Pezzi E

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Epm2a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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