Cela2a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cela2a
Entrez Gene ID	13706
Full Name	chymotrypsin-like elastase family, member 2A
Synonyms	Ela-2,Ela2,Ela2a
General protein information	Preferred Names chymotrypsin-like elastase family, member 2A Names chymotrypsin-like elastase family member 2A elastase 2A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 E1\|4 74.75 cM
Summary	This gene encodes a serine protease enzyme that hydrolyzes elastin. This gene is highly expressed in the pancreatic acinar cells where the encoded preproprotein undergoes processing including signal peptide cleavage to generate an inactive zymogen. The removal of N-terminal activation peptide from the zymogen by trypsin generates active elastase enzyme. This gene is also expressed in the mouse epidermis where it participates in pro-filaggrin processing. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007919.2	NP_031945.1	chymotrypsin-like elastase family member 2A precursor
NM_007919.3	NP_031945.1	chymotrypsin-like elastase family member 2A preproprotein

Pathways from BioSystems

Homologies

	ELA2B	XP_001083219.1
Bos taurus (cattle)	CELA2A	NP_777139.1
Rattus norvegicus (Norway rat)	Cela2a	NP_036685.1
Danio rerio (zebrafish)	ela2	NP_001132936.1
Gallus gallus (chicken)	CELA2A	NP_001027562.2
Xenopus tropicalis (tropical clawed frog)	LOC100498609	XP_002944523.2
Xenopus tropicalis (tropical clawed frog)	LOC100488535	XP_002942694.2
Pan troglodytes (chimpanzee)	CELA2A	XP_001156270.2
Mus musculus (house mouse)	Cela2a	NP_031945.1
Homo sapiens (human)	CELA2A	NP_254275.1
Danio rerio (zebrafish)	ela2l	NP_956180.1

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

Elastase-2, a Tissue Alternative Pathway for Angiotensin II Generation, Plays a Role in Circulatory Sympathovagal Balance in Mice.
Becari C, Durand MT, Guimaraes AO, Lataro RM, Prado CM, de Oliveira M, Candido SC, Pais P, Ribeiro MS, Bader M, Pesquero JB, Salgado MC, Salgado HC
Frontiers in physiology8170(2017)

The nuclear hormone receptor family member NR5A2 controls aspects of multipotent progenitor cell formation and acinar differentiation during pancreatic organogenesis.
Hale MA, Swift GH, Hoang CQ, Deering TG, Masui T, Lee YK, Xue J, MacDonald RJ
Development (Cambridge, England)141(16)3123-33(2014 Aug)

Dynamic expression of chymotrypsin-like elastase 1 over the course of murine lung development.
Liu S, Young SM, Varisco BM
American journal of physiology. Lung cellular and molecular physiology306(12)L1104-16(2014 Jun)

Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing.
Bonnart C, Deraison C, Lacroix M, Uchida Y, Besson C, Robin A, Briot A, Gonthier M, Lamant L, Dubus P, Monsarrat B, Hovnanian A
The Journal of clinical investigation120(3)871-82(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ELA2 as a major new epidermal protease involved in essential pathways for skin barrier function.
Title: Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing.

The following Cela2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cela2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01042	NM_007919.3 Latest version!	Mus musculus chymotrypsin-like elastase family, member 2A (Cela2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu01042	NM_007919.2	Mus musculus chymotrypsin-like elastase family, member 2A (Cela2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cela2a ( NM_007919.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031945.1 Mus musculus chymotrypsin-like elastase family, member 2A (Cela2a), mRNA.

Price & Availability↑

CloneID	OMu01042
Clone ID Related Accession (Same CDS sequence)	NM_007919.2 , NM_007919.3
Accession Version	NM_007919.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	chymotrypsin-like elastase family member 2A precursor
Comment

ATGATCAGGA CACTGCTGCT ATCTGCCTTG GTGGCTGGAG CCCTCAGCTG TGGGTACCCC 
ACTTATGAGG TGGAGGATGA TGTGAGCAGG GTAGTTGGGG GTCAAGAGGC CACACCCAAC 
ACCTGGCCCT GGCAGGTCTC CCTGCAGGTC CTTTCCTCCG GAAGGTGGCG CCACAACTGC 
GGAGGCTCCC TGGTGGCCAA CAACTGGGTT CTGACAGCTG CCCATTGCCT CAGCAACTAT 
CAGACCTACC GAGTGCTGCT GGGCGCACAC AGCCTCTCCA ACCCCGGAGC TGGCTCTGCT 
GCTGTTCAAG TCTCTAAGCT TGTGGTCCAC CAGAGGTGGA ACTCCCAAAA CGTCGGCAAT 
GGCTATGACA TTGCCTTAAT CAAACTGGCC AGCCCAGTGA CCCTGAGCAA GAACATCCAG 
ACAGCTTGCC TCCCACCCGC TGGCACCATT CTCCCGAGAA ACTATGTCTG CTATGTCACA 
GGCTGGGGCC TGCTGCAGAC CAATGGGAAC AGTCCTGACA CCCTGAGGCA GGGCCGCCTG 
CTGGTTGTGG ACTATGCCAC CTGCTCCAGC GCTAGCTGGT GGGGAAGCTC TGTGAAGTCC 
AGCATGGTGT GCGCTGGTGG CGACGGCGTG ACCTCCAGCT GCAATGGGGA CTCTGGCGGA 
CCACTGAATT GCCGGGCATC TAATGGCCAG TGGCAGGTGC ATGGCATCGT GAGCTTCGGC 
TCCTCTCTGG GCTGCAACTA CCCCCGCAAG CCATCCGTCT TCACCAGGGT CTCCAACTAC 
ATTGACTGGA TCAACTCGGT GATGGCAAGG AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031945.1
Translation	MIRTLLLSALVAGALSCGYPTYEVEDDVSRVVGGQEATPNTWPWQVSLQVLSSGRWRHNCGGSLVANNWVLTAAHCLSNYQTYRVLLGAHSLSNPGAGSAAVQVSKLVVHQRWNSQNVGNGYDIALIKLASPVTLSKNIQTACLPPAGTILPRNYVCYVTGWGLLQTNGNSPDTLRQGRLLVVDYATCSSASWWGSSVKSSMVCAGGDGVTSSCNGDSGGPLNCRASNGQWQVHGIVSFGSSLGCNYPRKPSVFTRVSNYIDWINSVMARN

Target ORF information:

RefSeq Version	NM_007919.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus chymotrypsin-like elastase family, member 2A (Cela2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007919.2

ORF Insert Sequence:

ATGATCAGGA CACTGCTGCT ATCTGCCTTG GTGGCTGGAG CCCTCAGCTG TGGGTACCCC 
ACTTATGAGG TGGAGGATGA TGTGAGCAGG GTAGTTGGGG GTCAAGAGGC CACACCCAAC 
ACCTGGCCCT GGCAGGTCTC CCTGCAGGTC CTTTCCTCCG GAAGGTGGCG CCACAACTGC 
GGAGGCTCCC TGGTGGCCAA CAACTGGGTT CTGACAGCTG CCCATTGCCT CAGCAACTAT 
CAGACCTACC GAGTGCTGCT GGGCGCACAC AGCCTCTCCA ACCCCGGAGC TGGCTCTGCT 
GCTGTTCAAG TCTCTAAGCT TGTGGTCCAC CAGAGGTGGA ACTCCCAAAA CGTCGGCAAT 
GGCTATGACA TTGCCTTAAT CAAACTGGCC AGCCCAGTGA CCCTGAGCAA GAACATCCAG 
ACAGCTTGCC TCCCACCCGC TGGCACCATT CTCCCGAGAA ACTATGTCTG CTATGTCACA 
GGCTGGGGCC TGCTGCAGAC CAATGGGAAC AGTCCTGACA CCCTGAGGCA GGGCCGCCTG 
CTGGTTGTGG ACTATGCCAC CTGCTCCAGC GCTAGCTGGT GGGGAAGCTC TGTGAAGTCC 
AGCATGGTGT GCGCTGGTGG CGACGGCGTG ACCTCCAGCT GCAATGGGGA CTCTGGCGGA 
CCACTGAATT GCCGGGCATC TAATGGCCAG TGGCAGGTGC ATGGCATCGT GAGCTTCGGC 
TCCTCTCTGG GCTGCAACTA CCCCCGCAAG CCATCCGTCT TCACCAGGGT CTCCAACTAC 
ATTGACTGGA TCAACTCGGT GATGGCAAGG AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cela2a ( NM_007919.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031945.1 Mus musculus chymotrypsin-like elastase family, member 2A (Cela2a), mRNA.

Price & Availability↑

CloneID	OMu01042
Clone ID Related Accession (Same CDS sequence)	NM_007919.2 , NM_007919.3
Accession Version	NM_007919.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	chymotrypsin-like elastase family member 2A preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL928577.21, CJ042571.1, CJ042553.1 and CJ042539.1. On Jul 16, 2016 this sequence version replaced NM_007919.2. ##Evidence-Data-START## Transcript exon combination :: X04573.1, BC026552.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAGGA CACTGCTGCT ATCTGCCTTG GTGGCTGGAG CCCTCAGCTG TGGGTACCCC 
ACTTATGAGG TGGAGGATGA TGTGAGCAGG GTAGTTGGGG GTCAAGAGGC CACACCCAAC 
ACCTGGCCCT GGCAGGTCTC CCTGCAGGTC CTTTCCTCCG GAAGGTGGCG CCACAACTGC 
GGAGGCTCCC TGGTGGCCAA CAACTGGGTT CTGACAGCTG CCCATTGCCT CAGCAACTAT 
CAGACCTACC GAGTGCTGCT GGGCGCACAC AGCCTCTCCA ACCCCGGAGC TGGCTCTGCT 
GCTGTTCAAG TCTCTAAGCT TGTGGTCCAC CAGAGGTGGA ACTCCCAAAA CGTCGGCAAT 
GGCTATGACA TTGCCTTAAT CAAACTGGCC AGCCCAGTGA CCCTGAGCAA GAACATCCAG 
ACAGCTTGCC TCCCACCCGC TGGCACCATT CTCCCGAGAA ACTATGTCTG CTATGTCACA 
GGCTGGGGCC TGCTGCAGAC CAATGGGAAC AGTCCTGACA CCCTGAGGCA GGGCCGCCTG 
CTGGTTGTGG ACTATGCCAC CTGCTCCAGC GCTAGCTGGT GGGGAAGCTC TGTGAAGTCC 
AGCATGGTGT GCGCTGGTGG CGACGGCGTG ACCTCCAGCT GCAATGGGGA CTCTGGCGGA 
CCACTGAATT GCCGGGCATC TAATGGCCAG TGGCAGGTGC ATGGCATCGT GAGCTTCGGC 
TCCTCTCTGG GCTGCAACTA CCCCCGCAAG CCATCCGTCT TCACCAGGGT CTCCAACTAC 
ATTGACTGGA TCAACTCGGT GATGGCAAGG AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031945.1
CDS	24..839
Translation	MIRTLLLSALVAGALSCGYPTYEVEDDVSRVVGGQEATPNTWPWQVSLQVLSSGRWRHNCGGSLVANNWVLTAAHCLSNYQTYRVLLGAHSLSNPGAGSAAVQVSKLVVHQRWNSQNVGNGYDIALIKLASPVTLSKNIQTACLPPAGTILPRNYVCYVTGWGLLQTNGNSPDTLRQGRLLVVDYATCSSASWWGSSVKSSMVCAGGDGVTSSCNGDSGGPLNCRASNGQWQVHGIVSFGSSLGCNYPRKPSVFTRVSNYIDWINSVMARN

Target ORF information:

RefSeq Version	NM_007919.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chymotrypsin-like elastase family, member 2A (Cela2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007919.3

ORF Insert Sequence:

ATGATCAGGA CACTGCTGCT ATCTGCCTTG GTGGCTGGAG CCCTCAGCTG TGGGTACCCC 
ACTTATGAGG TGGAGGATGA TGTGAGCAGG GTAGTTGGGG GTCAAGAGGC CACACCCAAC 
ACCTGGCCCT GGCAGGTCTC CCTGCAGGTC CTTTCCTCCG GAAGGTGGCG CCACAACTGC 
GGAGGCTCCC TGGTGGCCAA CAACTGGGTT CTGACAGCTG CCCATTGCCT CAGCAACTAT 
CAGACCTACC GAGTGCTGCT GGGCGCACAC AGCCTCTCCA ACCCCGGAGC TGGCTCTGCT 
GCTGTTCAAG TCTCTAAGCT TGTGGTCCAC CAGAGGTGGA ACTCCCAAAA CGTCGGCAAT 
GGCTATGACA TTGCCTTAAT CAAACTGGCC AGCCCAGTGA CCCTGAGCAA GAACATCCAG 
ACAGCTTGCC TCCCACCCGC TGGCACCATT CTCCCGAGAA ACTATGTCTG CTATGTCACA 
GGCTGGGGCC TGCTGCAGAC CAATGGGAAC AGTCCTGACA CCCTGAGGCA GGGCCGCCTG 
CTGGTTGTGG ACTATGCCAC CTGCTCCAGC GCTAGCTGGT GGGGAAGCTC TGTGAAGTCC 
AGCATGGTGT GCGCTGGTGG CGACGGCGTG ACCTCCAGCT GCAATGGGGA CTCTGGCGGA 
CCACTGAATT GCCGGGCATC TAATGGCCAG TGGCAGGTGC ATGGCATCGT GAGCTTCGGC 
TCCTCTCTGG GCTGCAACTA CCCCCGCAAG CCATCCGTCT TCACCAGGGT CTCCAACTAC 
ATTGACTGGA TCAACTCGGT GATGGCAAGG AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

Elastase-2, a Tissue Alternative Pathway for Angiotensin II Generation, Plays a Role in Circulatory Sympathovagal Balance in Mice.
Frontiers in physiology8170(2017)
Becari C,Durand MT,Guimaraes AO,Lataro RM,Prado CM,de Oliveira M,Candido SC,Pais P,Ribeiro MS,Bader M,Pesquero JB,Salgado MC,Salgado HC

The nuclear hormone receptor family member NR5A2 controls aspects of multipotent progenitor cell formation and acinar differentiation during pancreatic organogenesis.
Development (Cambridge, England)141(16)3123-33(2014 Aug)
Hale MA,Swift GH,Hoang CQ,Deering TG,Masui T,Lee YK,Xue J,MacDonald RJ

Dynamic expression of chymotrypsin-like elastase 1 over the course of murine lung development.
American journal of physiology. Lung cellular and molecular physiology306(12)L1104-16(2014 Jun)
Liu S,Young SM,Varisco BM

Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing.
The Journal of clinical investigation120(3)871-82(2010 Mar)
Bonnart C,Deraison C,Lacroix M,Uchida Y,Besson C,Robin A,Briot A,Gonthier M,Lamant L,Dubus P,Monsarrat B,Hovnanian A

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Cela2a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報