Ebp cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ebp
Entrez Gene ID	13595
Full Name	phenylalkylamine Ca2+ antagonist (emopamil) binding protein
Synonyms	AI255399,Pabp,SI,Td,mSI
General protein information	Preferred Names phenylalkylamine Ca2+ antagonist (emopamil) binding protein Names 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase D8-D7 sterol isomerase cholestenol Delta-isomerase delta(8)-Delta(7) sterol isomerase tattered
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A1.1\|X 3.7 cM
Summary	This gene encodes a transmembrane protein that localizes to the endoplasmic reticulum. This protein catalyses the conversion of delta8 to delta7 sterols, an important step in sterol biosynthesis. Mutations in this gene are responsible for the mouse tattered mutant phenotype. Tattered males are embryonic lethal, while heterozygous females have developmental defects. Deficiency of the related gene in human causes X-linked dominant chondrodysplasia punctata. [provided by RefSeq, May 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007898.3	NP_031924.1	3-beta-hydroxysteroid-Delta(8), Delta(7)-isomerase

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os01g0103600	NP_001041757.1
Bos taurus (cattle)	EBP	NP_001029672.1
Rattus norvegicus (Norway rat)	Ebp	NP_476478.1
Magnaporthe oryzae (rice blast fungus)	MGG_04574	XP_003713603.1
Homo sapiens (human)	EBP	NP_006570.1
Canis lupus familiaris (dog)	EBP	XP_851024.1
Mus musculus (house mouse)	Ebp	NP_031924.1
Danio rerio (zebrafish)	ebp	NP_001002328.1
Arabidopsis thaliana (thale cress)	HYD1	NP_173433.1

Gene Ontology
Bibliography

Related articles in PubMed

HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes.
Jin X, Xu Z, Cao J, Yan R, Xu R, Ran R, Ma Y, Cai W, Fan R, Zhang Y, Zhou X, Li Y
International journal of molecular medicine39(6)1409-1420(2017 Jun)

A compendium of expression patterns of cholesterol biosynthetic enzymes in the mouse embryo.
?i?ecio?lu M, Budak H, Geffers L, ?ankaya M, ?iftci M, Thaller C, Eichele G, K?frevio?lu ??, ?zdemir H
Journal of lipid research56(8)1551-9(2015 Aug)

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Brommage R, Liu J, Hansen GM, Kirkpatrick LL, Potter DG, Sands AT, Zambrowicz B, Powell DR, Vogel P
Bone research214034(2014)

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Evsikov AV, Graber JH, Brockman JM, Hampl A, Holbrook AE, Singh P, Eppig JJ, Solter D, Knowles BB
Genes & development20(19)2713-27(2006 Oct)

Embryonic hematopoiesis defect in the tattered-Hokkaido (Tdho) mouse.
Seo KW, Kon Y, Watanabe T
Comparative medicine50(1)27-31(2000 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mouse Tdho abnormality results from double point mutations of the emopamil binding protein gene (Ebp).
Title: Mouse Tdho abnormality results from double point mutations of the emopamil binding protein gene (Ebp).

The following Ebp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ebp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01015	NM_007898.3 Latest version!	Mus musculus phenylalkylamine Ca2+ antagonist (emopamil) binding protein (Ebp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ebp ( NM_007898.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031924.1 Mus musculus phenylalkylamine Ca2+ antagonist (emopamil) binding protein (Ebp), mRNA.

Price & Availability↑

CloneID	OMu01015
Clone ID Related Accession (Same CDS sequence)	NM_007898.3
Accession Version	NM_007898.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	3-beta-hydroxysteroid-Delta(8), Delta(7)-isomerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL663032.9, AK027977.1 and DV661455.1. On May 7, 2015 this sequence version replaced NM_007898.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCACCA ATACGGTCCC CTTGCACCCG TACTGGCCCA GGCACCTGAA GCTGGACAAC 
TTCGTGCCTA ATGACCTCCC GACTTCGCAT ATCCTGGTTG GCCTCTTCTC CATCTCTGGG 
GGCCTAATTG TGATCACGTG GCTGTTGTCT AGCCGAGCTT CCGTCGTCCC ACTTGGAGCT 
GGGCGGCGAC TGGCCTTGTG CTGGTTTGCT GTGTGTACCT TCATTCACCT TGTGATCGAG 
GGCTGGTTCT CTCTCTACAA TGGCATCCTT TTAGAAGACC AAGCCTTCTT ATCCCAACTC 
TGGAAAGAGT ATTCCAAGGG AGATAGCCGA TATATCCTTA GTGACAGCTT CGTCGTCTGT 
ATGGAGACTG TCACAGCTTG TCTCTGGGGA CCACTCAGCC TATGGGTAGT GATTGCCTTT 
CTCCGCCAAC AGCCCTTCCG CTTTGTCCTA CAGCTTGTGG TGTCTATGGG CCAGATATAC 
GGGGATGTGC TGTACTTCCT GACAGAGCTA CACGAAGGAC TCCAGCATGG GGAGATAGGC 
CACCCCGTTT ATTTCTGGTT CTATTTTGTT TTCCTGAATG CTGTATGGTT GGTGATACCA 
AGCATCCTTG TGCTTGATGC CATAAAGCAT CTCACTAGTG CCCAGAGCGT GCTGGACAGC 
AAAGTCATGA AAATTAAGAG CAAGCATAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031924.1
CDS	770..1462
Translation	MTTNTVPLHPYWPRHLKLDNFVPNDLPTSHILVGLFSISGGLIVITWLLSSRASVVPLGAGRRLALCWFAVCTFIHLVIEGWFSLYNGILLEDQAFLSQLWKEYSKGDSRYILSDSFVVCMETVTACLWGPLSLWVVIAFLRQQPFRFVLQLVVSMGQIYGDVLYFLTELHEGLQHGEIGHPVYFWFYFVFLNAVWLVIPSILVLDAIKHLTSAQSVLDSKVMKIKSKHN

Target ORF information:

RefSeq Version	NM_007898.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phenylalkylamine Ca2+ antagonist (emopamil) binding protein (Ebp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007898.3

ORF Insert Sequence:

ATGACCACCA ATACGGTCCC CTTGCACCCG TACTGGCCCA GGCACCTGAA GCTGGACAAC 
TTCGTGCCTA ATGACCTCCC GACTTCGCAT ATCCTGGTTG GCCTCTTCTC CATCTCTGGG 
GGCCTAATTG TGATCACGTG GCTGTTGTCT AGCCGAGCTT CCGTCGTCCC ACTTGGAGCT 
GGGCGGCGAC TGGCCTTGTG CTGGTTTGCT GTGTGTACCT TCATTCACCT TGTGATCGAG 
GGCTGGTTCT CTCTCTACAA TGGCATCCTT TTAGAAGACC AAGCCTTCTT ATCCCAACTC 
TGGAAAGAGT ATTCCAAGGG AGATAGCCGA TATATCCTTA GTGACAGCTT CGTCGTCTGT 
ATGGAGACTG TCACAGCTTG TCTCTGGGGA CCACTCAGCC TATGGGTAGT GATTGCCTTT 
CTCCGCCAAC AGCCCTTCCG CTTTGTCCTA CAGCTTGTGG TGTCTATGGG CCAGATATAC 
GGGGATGTGC TGTACTTCCT GACAGAGCTA CACGAAGGAC TCCAGCATGG GGAGATAGGC 
CACCCCGTTT ATTTCTGGTT CTATTTTGTT TTCCTGAATG CTGTATGGTT GGTGATACCA 
AGCATCCTTG TGCTTGATGC CATAAAGCAT CTCACTAGTG CCCAGAGCGT GCTGGACAGC 
AAAGTCATGA AAATTAAGAG CAAGCATAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes.
International journal of molecular medicine39(6)1409-1420(2017 Jun)
Jin X,Xu Z,Cao J,Yan R,Xu R,Ran R,Ma Y,Cai W,Fan R,Zhang Y,Zhou X,Li Y

A compendium of expression patterns of cholesterol biosynthetic enzymes in the mouse embryo.
Journal of lipid research56(8)1551-9(2015 Aug)
?i?ecio?lu M,Budak H,Geffers L,?ankaya M,?iftci M,Thaller C,Eichele G,K?frevio?lu ??,?zdemir H

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Bone research214034(2014)
Brommage R,Liu J,Hansen GM,Kirkpatrick LL,Potter DG,Sands AT,Zambrowicz B,Powell DR,Vogel P

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Genes & development20(19)2713-27(2006 Oct)
Evsikov AV,Graber JH,Brockman JM,Hampl A,Holbrook AE,Singh P,Eppig JJ,Solter D,Knowles BB

Embryonic hematopoiesis defect in the tattered-Hokkaido (Tdho) mouse.
Comparative medicine50(1)27-31(2000 Feb)
Seo KW,Kon Y,Watanabe T

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Ebp cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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