EBF2 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	EBF2
Entrez Gene ID	13592
Full Name	early B cell factor 2
Synonyms	D14Ggc1e,EBF-2,Mmot1,O/E-3,OE-3
General protein information	Preferred Names early B cell factor 2 Names transcription factor COE2 MET-mesencephalon-olfactory TF1 MET-mesencephalon-olfactory transcription factor 1 Olf-1/EBF-like 3 metencephalon-mesencephalon-olfactory transcription factor 1
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 14 Map Location: 14\|14 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_010095.6	NP_034225.1	transcription factor COE2
XM_011244945.2	XP_011243247.1	transcription factor COE2 isoform X1
NM_001276387.1	NP_001263316.1	transcription factor COE2
NM_001360202.1	NP_001347131.1	transcription factor COE2
XM_011244945.1	XP_011243247.1	transcription factor COE2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EBF2	XP_001161196.1
Mus musculus (house mouse)	Ebf2	NP_034225.1
Gallus gallus (chicken)	EBF2	NP_001158008.1
Homo sapiens (human)	EBF2	NP_073150.2
Danio rerio (zebrafish)	coe2	NP_571493.1
	EBF2	XP_001108018.1
Rattus norvegicus (Norway rat)	Ebf2	NP_001101853.1
Canis lupus familiaris (dog)	EBF2	XP_534569.2
Bos taurus (cattle)	EBF2	NP_001071407.1
Xenopus tropicalis (tropical clawed frog)	ebf2	NP_989200.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Memic F, Knoflach V, Morarach K, Sadler R, Laranjeira C, Hjerling-Leffler J, Sundstr?m E, Pachnis V, Marklund U
Gastroenterology(2017 Oct)

EBF2 transcriptionally regulates brown adipogenesis via the histone reader DPF3 and the BAF chromatin remodeling complex.
Shapira SN, Lim HW, Rajakumari S, Sakers AP, Ishibashi J, Harms MJ, Won KJ, Seale P
Genes & development31(7)660-673(2017 Apr)

Comparative Transcriptomic and Epigenomic Analyses Reveal New Regulators of Murine Brown Adipogenesis.
Brunmeir R, Wu J, Peng X, Kim SY, Julien SG, Zhang Q, Xie W, Xu F
PLoS genetics12(12)e1006474(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we show that SIX1 binds to adipogenic and brown marker genes and interacts with C/EBPa, C/EBPb and EBF2, suggesting their functional cooperation during adipogenesis.
Title: Comparative Transcriptomic and Epigenomic Analyses Reveal New Regulators of Murine Brown Adipogenesis.

Data indicate that Contactin-1 expression is dependent upon EBF factors; Cntn1 gene belongs to the expanding regulatory cascade driven by these transcriptional regulators so that changes in its activation may contribute to the Ebf2 null mutant phenotype
Title: The gene encoding the mouse contactin-1 axonal glycoprotein is regulated by the collier/Olf1/EBF family early B-Cell factor 2 transcription factor.

Study identified Ebf2 as a novel and important regulator selectively required for midbrain periaqueductal gray matter dopamine neuron development
Title: Ebf2 is required for development of dopamine neurons in the midbrain periaqueductal gray matter of mouse.

Results indicate that early B cell factor 2 (Ebf2) specifically marks and regulates the molecular profile of brown preadipose cells.
Title: Ebf2 is a selective marker of brown and beige adipogenic precursor cells.

Blnc1 forms a ribonucleoprotein complex with transcription factor EBF2 to stimulate the thermogenic gene program. Further, Blnc1 itself is a target of EBF2, thereby forming a feedforward regulatory loop to drive adipogenesis toward thermogenic phenotype.
Title: A long noncoding RNA transcriptional regulatory circuit drives thermogenic adipocyte differentiation.

The following EBF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EBF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16972	NM_001276387.1 Latest version!	Mus musculus early B cell factor 2 (Ebf2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu16972	NM_001360202.1 Latest version!	Mus musculus early B cell factor 2 (Ebf2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu16972	NM_010095.6 Latest version!	Mus musculus early B cell factor 2 (Ebf2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu16972	XM_011244945.2 Latest version!	Mus musculus early B cell factor 2 (Ebf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
View Old Accession Versions >>
OMu16972	XM_011244945.1	Mus musculus early B cell factor 2 (Ebf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EBF2 ( NM_010095.6 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_034225.1 Mus musculus early B cell factor 2 (Ebf2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16972
Clone ID Related Accession (Same CDS sequence)	NM_010095.6 , XM_011244945.2 , NM_001276387.1 , NM_001360202.1 , XM_011244945.1
Accession Version	NM_010095.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	transcription factor COE2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK053013.1, AK139262.1, BB669324.1 and CT010493.12. On Feb 6, 2013 this sequence version replaced NM_010095.5. Transcript Variant: This variant (2) uses an alternate splice site in 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK053013.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGGGA TTCAAGATAC GCTAGGAAGA GGACCAGCTC TGAAAGACAA GTCGCTGGGT 
GCCGAGATGG ATTCGGTCAG GTCCTGGGTC CGGAATGTCG GCGTGGTGGA CGCCAATGTC 
GCGGCTCAGA GCGGAGTTGC TCTGTCCCGG GCCCATTTTG AAAAGCAACC TCCTTCAAAC 
TTGAGGAAAT CCAACTTCTT TCATTTTGTC CTGGCGCTCT ACGACAGGCA GGGACAGCCG 
GTGGAGATTG AGCGCACAGC CTTCGTGGAC TTTGTGGAGA ATGACAAAGA GCAAGGCAAT 
GAGAAGACCA ACAATGGTAC TCACTACAAG TTACAGCTCC TTTACAGCAA CGGTGTCCGC 
ACGGAGCAGG ACCTGTATGT CAGGCTCATC GACTCTGTCA CCAAACAGCC CATAGCCTAT 
GAGGGACAGA ATAAGAATCC CGAAATGTGC AGAGTTCTGC TGACACACGA GGTGATGTGC 
AGTCGATGCT GTGAGAAGAA GAGCTGCGGG AACCGGAACG AGACCCCTTC AGACCCGGTC 
ATCATCGACA GATTCTTTTT AAAATTTTTC CTGAAGTGCA ATCAGAATTG TTTGAAAACA 
GCGGGAAACC CAAGGGACAT GAGGCGGTTC CAGGTCGTGT TGTCCACAAC AGTAAATGTG 
GATGGACATG TGCTGGCTGT TTCTGACAAC ATGTTTGTTC ATAACAATTC TAAGCATGGA 
AGGAGAGCAA GAAGGCTTGA CCCATCTGAA GCTACACCCT GCATCAAAGC CATCAGCCCA 
AGTGAAGGCT GGACCACAGG AGGGGCCATG GTCATCATCA TTGGTGACAA CTTTTTTGAT 
GGTCTCCAGG TTGTGTTTGG TACCATGCTT GTATGGAGTG AGCTCATAAC CCCTCATGCC 
ATCAGAGTAC AGACCCCTCC CCGGCATATC CCTGGAGTTG TGGAAGTGAC ACTATCTTAT 
AAATCCAAAC AGTTCTGCAA AGGAGCCCCG GGCAGGTTCA TCTACACAGC CTTAAATGAA 
CCCACCATAG ACTATGGCTT CCAAAGATTG CAGAAAGTCA TCCCAAGGCA CCCAGGTGAC 
CCTGAGAGGC TAGCTAAGGA AATGCTGTTG AAAAGAGCTG CGGACCTGGT GGAAGCCCTC 
TACGGCACAC CACACAATAA TCAGGACATC ATTTTAAAGC GTGCTGCAGA CATTGCTGAA 
GCCCTGTACA GTGTCCCCAG GAACCCCAGC CAGATTCCAG CTCTTTCCAG CTCCCCAGCT 
CACAGCGGTA TGATGGGTAT CAACTCTTAC GGCAGCCAGC TCGGCGTCAG CATTTCAGAG 
TCCACACAAG GAAATAACCA AGGATACATC CGCAACACAA GCAGCATCTC CCCGCGGGGA 
TACTCCTCCA GCTCCACGCC TCAGCAATCT AATTACAGCA CCTCCAGCAA CAGCATGAAC 
GGCTACAGCA ATGTTCCTAT GGCCAACCTG GGTGTGCCAG GATCCCCGGG ATTTCTGAAT 
GGCTCACCCA CGGGCTCCCC ATATGGAATC ATGTCTTCAA GCCCCACCGT GGGATCCTCC 
AGCACGTCCT CCATCCTCCC GTTCTCCTCT TCAGTTTTTC CTGCTGTCAA ACAGAAGAGT 
GCCTTTGCCC CTGTCATCAG GCCCCAAGGC TCCCCATCGC CTGCCTGCTC CAGCGGCAAT 
GGGAACGGAT TCAGAGCCAT GACAGGACTT GTTGTTCCCC CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034225.1
CDS	970..2697
Translation	MFGIQDTLGRGPALKDKSLGAEMDSVRSWVRNVGVVDANVAAQSGVALSRAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVDFVENDKEQGNEKTNNGTHYKLQLLYSNGVRTEQDLYVRLIDSVTKQPIAYEGQNKNPEMCRVLLTHEVMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKTAGNPRDMRRFQVVLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGAMVIIIGDNFFDGLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLAKEMLLKRAADLVEALYGTPHNNQDIILKRAADIAEALYSVPRNPSQIPALSSSPAHSGMMGINSYGSQLGVSISESTQGNNQGYIRNTSSISPRGYSSSSTPQQSNYSTSSNSMNGYSNVPMANLGVPGSPGFLNGSPTGSPYGIMSSSPTVGSSSTSSILPFSSSVFPAVKQKSAFAPVIRPQGSPSPACSSGNGNGFRAMTGLVVPPM

Target ORF information:

RefSeq Version	NM_010095.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus early B cell factor 2 (Ebf2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010095.6

ORF Insert Sequence:

ATGTTTGGGA TTCAAGATAC GCTAGGAAGA GGACCAGCTC TGAAAGACAA GTCGCTGGGT 
GCCGAGATGG ATTCGGTCAG GTCCTGGGTC CGGAATGTCG GCGTGGTGGA CGCCAATGTC 
GCGGCTCAGA GCGGAGTTGC TCTGTCCCGG GCCCATTTTG AAAAGCAACC TCCTTCAAAC 
TTGAGGAAAT CCAACTTCTT TCATTTTGTC CTGGCGCTCT ACGACAGGCA GGGACAGCCG 
GTGGAGATTG AGCGCACAGC CTTCGTGGAC TTTGTGGAGA ATGACAAAGA GCAAGGCAAT 
GAGAAGACCA ACAATGGTAC TCACTACAAG TTACAGCTCC TTTACAGCAA CGGTGTCCGC 
ACGGAGCAGG ACCTGTATGT CAGGCTCATC GACTCTGTCA CCAAACAGCC CATAGCCTAT 
GAGGGACAGA ATAAGAATCC CGAAATGTGC AGAGTTCTGC TGACACACGA GGTGATGTGC 
AGTCGATGCT GTGAGAAGAA GAGCTGCGGG AACCGGAACG AGACCCCTTC AGACCCGGTC 
ATCATCGACA GATTCTTTTT AAAATTTTTC CTGAAGTGCA ATCAGAATTG TTTGAAAACA 
GCGGGAAACC CAAGGGACAT GAGGCGGTTC CAGGTCGTGT TGTCCACAAC AGTAAATGTG 
GATGGACATG TGCTGGCTGT TTCTGACAAC ATGTTTGTTC ATAACAATTC TAAGCATGGA 
AGGAGAGCAA GAAGGCTTGA CCCATCTGAA GCTACACCCT GCATCAAAGC CATCAGCCCA 
AGTGAAGGCT GGACCACAGG AGGGGCCATG GTCATCATCA TTGGTGACAA CTTTTTTGAT 
GGTCTCCAGG TTGTGTTTGG TACCATGCTT GTATGGAGTG AGCTCATAAC CCCTCATGCC 
ATCAGAGTAC AGACCCCTCC CCGGCATATC CCTGGAGTTG TGGAAGTGAC ACTATCTTAT 
AAATCCAAAC AGTTCTGCAA AGGAGCCCCG GGCAGGTTCA TCTACACAGC CTTAAATGAA 
CCCACCATAG ACTATGGCTT CCAAAGATTG CAGAAAGTCA TCCCAAGGCA CCCAGGTGAC 
CCTGAGAGGC TAGCTAAGGA AATGCTGTTG AAAAGAGCTG CGGACCTGGT GGAAGCCCTC 
TACGGCACAC CACACAATAA TCAGGACATC ATTTTAAAGC GTGCTGCAGA CATTGCTGAA 
GCCCTGTACA GTGTCCCCAG GAACCCCAGC CAGATTCCAG CTCTTTCCAG CTCCCCAGCT 
CACAGCGGTA TGATGGGTAT CAACTCTTAC GGCAGCCAGC TCGGCGTCAG CATTTCAGAG 
TCCACACAAG GAAATAACCA AGGATACATC CGCAACACAA GCAGCATCTC CCCGCGGGGA 
TACTCCTCCA GCTCCACGCC TCAGCAATCT AATTACAGCA CCTCCAGCAA CAGCATGAAC 
GGCTACAGCA ATGTTCCTAT GGCCAACCTG GGTGTGCCAG GATCCCCGGG ATTTCTGAAT 
GGCTCACCCA CGGGCTCCCC ATATGGAATC ATGTCTTCAA GCCCCACCGT GGGATCCTCC 
AGCACGTCCT CCATCCTCCC GTTCTCCTCT TCAGTTTTTC CTGCTGTCAA ACAGAAGAGT 
GCCTTTGCCC CTGTCATCAG GCCCCAAGGC TCCCCATCGC CTGCCTGCTC CAGCGGCAAT 
GGGAACGGAT TCAGAGCCAT GACAGGACTT GTTGTTCCCC CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EBF2 ( NM_001276387.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001263316.1 Mus musculus early B cell factor 2 (Ebf2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16972
Clone ID Related Accession (Same CDS sequence)	NM_010095.6 , XM_011244945.2 , NM_001276387.1 , NM_001360202.1 , XM_011244945.1
Accession Version	NM_001276387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	transcription factor COE2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK053013.1, AK139262.1, BB669324.1 and CT010493.12. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK139262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGGGA TTCAAGATAC GCTAGGAAGA GGACCAGCTC TGAAAGACAA GTCGCTGGGT 
GCCGAGATGG ATTCGGTCAG GTCCTGGGTC CGGAATGTCG GCGTGGTGGA CGCCAATGTC 
GCGGCTCAGA GCGGAGTTGC TCTGTCCCGG GCCCATTTTG AAAAGCAACC TCCTTCAAAC 
TTGAGGAAAT CCAACTTCTT TCATTTTGTC CTGGCGCTCT ACGACAGGCA GGGACAGCCG 
GTGGAGATTG AGCGCACAGC CTTCGTGGAC TTTGTGGAGA ATGACAAAGA GCAAGGCAAT 
GAGAAGACCA ACAATGGTAC TCACTACAAG TTACAGCTCC TTTACAGCAA CGGTGTCCGC 
ACGGAGCAGG ACCTGTATGT CAGGCTCATC GACTCTGTCA CCAAACAGCC CATAGCCTAT 
GAGGGACAGA ATAAGAATCC CGAAATGTGC AGAGTTCTGC TGACACACGA GGTGATGTGC 
AGTCGATGCT GTGAGAAGAA GAGCTGCGGG AACCGGAACG AGACCCCTTC AGACCCGGTC 
ATCATCGACA GATTCTTTTT AAAATTTTTC CTGAAGTGCA ATCAGAATTG TTTGAAAACA 
GCGGGAAACC CAAGGGACAT GAGGCGGTTC CAGGTCGTGT TGTCCACAAC AGTAAATGTG 
GATGGACATG TGCTGGCTGT TTCTGACAAC ATGTTTGTTC ATAACAATTC TAAGCATGGA 
AGGAGAGCAA GAAGGCTTGA CCCATCTGAA GCTACACCCT GCATCAAAGC CATCAGCCCA 
AGTGAAGGCT GGACCACAGG AGGGGCCATG GTCATCATCA TTGGTGACAA CTTTTTTGAT 
GGTCTCCAGG TTGTGTTTGG TACCATGCTT GTATGGAGTG AGCTCATAAC CCCTCATGCC 
ATCAGAGTAC AGACCCCTCC CCGGCATATC CCTGGAGTTG TGGAAGTGAC ACTATCTTAT 
AAATCCAAAC AGTTCTGCAA AGGAGCCCCG GGCAGGTTCA TCTACACAGC CTTAAATGAA 
CCCACCATAG ACTATGGCTT CCAAAGATTG CAGAAAGTCA TCCCAAGGCA CCCAGGTGAC 
CCTGAGAGGC TAGCTAAGGA AATGCTGTTG AAAAGAGCTG CGGACCTGGT GGAAGCCCTC 
TACGGCACAC CACACAATAA TCAGGACATC ATTTTAAAGC GTGCTGCAGA CATTGCTGAA 
GCCCTGTACA GTGTCCCCAG GAACCCCAGC CAGATTCCAG CTCTTTCCAG CTCCCCAGCT 
CACAGCGGTA TGATGGGTAT CAACTCTTAC GGCAGCCAGC TCGGCGTCAG CATTTCAGAG 
TCCACACAAG GAAATAACCA AGGATACATC CGCAACACAA GCAGCATCTC CCCGCGGGGA 
TACTCCTCCA GCTCCACGCC TCAGCAATCT AATTACAGCA CCTCCAGCAA CAGCATGAAC 
GGCTACAGCA ATGTTCCTAT GGCCAACCTG GGTGTGCCAG GATCCCCGGG ATTTCTGAAT 
GGCTCACCCA CGGGCTCCCC ATATGGAATC ATGTCTTCAA GCCCCACCGT GGGATCCTCC 
AGCACGTCCT CCATCCTCCC GTTCTCCTCT TCAGTTTTTC CTGCTGTCAA ACAGAAGAGT 
GCCTTTGCCC CTGTCATCAG GCCCCAAGGC TCCCCATCGC CTGCCTGCTC CAGCGGCAAT 
GGGAACGGAT TCAGAGCCAT GACAGGACTT GTTGTTCCCC CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263316.1
CDS	975..2702
Translation	MFGIQDTLGRGPALKDKSLGAEMDSVRSWVRNVGVVDANVAAQSGVALSRAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVDFVENDKEQGNEKTNNGTHYKLQLLYSNGVRTEQDLYVRLIDSVTKQPIAYEGQNKNPEMCRVLLTHEVMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKTAGNPRDMRRFQVVLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGAMVIIIGDNFFDGLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLAKEMLLKRAADLVEALYGTPHNNQDIILKRAADIAEALYSVPRNPSQIPALSSSPAHSGMMGINSYGSQLGVSISESTQGNNQGYIRNTSSISPRGYSSSSTPQQSNYSTSSNSMNGYSNVPMANLGVPGSPGFLNGSPTGSPYGIMSSSPTVGSSSTSSILPFSSSVFPAVKQKSAFAPVIRPQGSPSPACSSGNGNGFRAMTGLVVPPM

Target ORF information:

RefSeq Version	NM_001276387.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus early B cell factor 2 (Ebf2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276387.1

ORF Insert Sequence:

ATGTTTGGGA TTCAAGATAC GCTAGGAAGA GGACCAGCTC TGAAAGACAA GTCGCTGGGT 
GCCGAGATGG ATTCGGTCAG GTCCTGGGTC CGGAATGTCG GCGTGGTGGA CGCCAATGTC 
GCGGCTCAGA GCGGAGTTGC TCTGTCCCGG GCCCATTTTG AAAAGCAACC TCCTTCAAAC 
TTGAGGAAAT CCAACTTCTT TCATTTTGTC CTGGCGCTCT ACGACAGGCA GGGACAGCCG 
GTGGAGATTG AGCGCACAGC CTTCGTGGAC TTTGTGGAGA ATGACAAAGA GCAAGGCAAT 
GAGAAGACCA ACAATGGTAC TCACTACAAG TTACAGCTCC TTTACAGCAA CGGTGTCCGC 
ACGGAGCAGG ACCTGTATGT CAGGCTCATC GACTCTGTCA CCAAACAGCC CATAGCCTAT 
GAGGGACAGA ATAAGAATCC CGAAATGTGC AGAGTTCTGC TGACACACGA GGTGATGTGC 
AGTCGATGCT GTGAGAAGAA GAGCTGCGGG AACCGGAACG AGACCCCTTC AGACCCGGTC 
ATCATCGACA GATTCTTTTT AAAATTTTTC CTGAAGTGCA ATCAGAATTG TTTGAAAACA 
GCGGGAAACC CAAGGGACAT GAGGCGGTTC CAGGTCGTGT TGTCCACAAC AGTAAATGTG 
GATGGACATG TGCTGGCTGT TTCTGACAAC ATGTTTGTTC ATAACAATTC TAAGCATGGA 
AGGAGAGCAA GAAGGCTTGA CCCATCTGAA GCTACACCCT GCATCAAAGC CATCAGCCCA 
AGTGAAGGCT GGACCACAGG AGGGGCCATG GTCATCATCA TTGGTGACAA CTTTTTTGAT 
GGTCTCCAGG TTGTGTTTGG TACCATGCTT GTATGGAGTG AGCTCATAAC CCCTCATGCC 
ATCAGAGTAC AGACCCCTCC CCGGCATATC CCTGGAGTTG TGGAAGTGAC ACTATCTTAT 
AAATCCAAAC AGTTCTGCAA AGGAGCCCCG GGCAGGTTCA TCTACACAGC CTTAAATGAA 
CCCACCATAG ACTATGGCTT CCAAAGATTG CAGAAAGTCA TCCCAAGGCA CCCAGGTGAC 
CCTGAGAGGC TAGCTAAGGA AATGCTGTTG AAAAGAGCTG CGGACCTGGT GGAAGCCCTC 
TACGGCACAC CACACAATAA TCAGGACATC ATTTTAAAGC GTGCTGCAGA CATTGCTGAA 
GCCCTGTACA GTGTCCCCAG GAACCCCAGC CAGATTCCAG CTCTTTCCAG CTCCCCAGCT 
CACAGCGGTA TGATGGGTAT CAACTCTTAC GGCAGCCAGC TCGGCGTCAG CATTTCAGAG 
TCCACACAAG GAAATAACCA AGGATACATC CGCAACACAA GCAGCATCTC CCCGCGGGGA 
TACTCCTCCA GCTCCACGCC TCAGCAATCT AATTACAGCA CCTCCAGCAA CAGCATGAAC 
GGCTACAGCA ATGTTCCTAT GGCCAACCTG GGTGTGCCAG GATCCCCGGG ATTTCTGAAT 
GGCTCACCCA CGGGCTCCCC ATATGGAATC ATGTCTTCAA GCCCCACCGT GGGATCCTCC 
AGCACGTCCT CCATCCTCCC GTTCTCCTCT TCAGTTTTTC CTGCTGTCAA ACAGAAGAGT 
GCCTTTGCCC CTGTCATCAG GCCCCAAGGC TCCCCATCGC CTGCCTGCTC CAGCGGCAAT 
GGGAACGGAT TCAGAGCCAT GACAGGACTT GTTGTTCCCC CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EBF2 ( NM_001360202.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001347131.1 Mus musculus early B cell factor 2 (Ebf2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu16972
Clone ID Related Accession (Same CDS sequence)	NM_010095.6 , XM_011244945.2 , NM_001276387.1 , NM_001360202.1 , XM_011244945.1
Accession Version	NM_001360202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	transcription factor COE2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC050922.1, CT010493.12 and BM932499.1. On Jan 30, 2018 this sequence version replaced XM_011244945.2. Transcript Variant: This variant (3) differs in 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050922.1, SRR1660821.118209.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGGGA TTCAAGATAC GCTAGGAAGA GGACCAGCTC TGAAAGACAA GTCGCTGGGT 
GCCGAGATGG ATTCGGTCAG GTCCTGGGTC CGGAATGTCG GCGTGGTGGA CGCCAATGTC 
GCGGCTCAGA GCGGAGTTGC TCTGTCCCGG GCCCATTTTG AAAAGCAACC TCCTTCAAAC 
TTGAGGAAAT CCAACTTCTT TCATTTTGTC CTGGCGCTCT ACGACAGGCA GGGACAGCCG 
GTGGAGATTG AGCGCACAGC CTTCGTGGAC TTTGTGGAGA ATGACAAAGA GCAAGGCAAT 
GAGAAGACCA ACAATGGTAC TCACTACAAG TTACAGCTCC TTTACAGCAA CGGTGTCCGC 
ACGGAGCAGG ACCTGTATGT CAGGCTCATC GACTCTGTCA CCAAACAGCC CATAGCCTAT 
GAGGGACAGA ATAAGAATCC CGAAATGTGC AGAGTTCTGC TGACACACGA GGTGATGTGC 
AGTCGATGCT GTGAGAAGAA GAGCTGCGGG AACCGGAACG AGACCCCTTC AGACCCGGTC 
ATCATCGACA GATTCTTTTT AAAATTTTTC CTGAAGTGCA ATCAGAATTG TTTGAAAACA 
GCGGGAAACC CAAGGGACAT GAGGCGGTTC CAGGTCGTGT TGTCCACAAC AGTAAATGTG 
GATGGACATG TGCTGGCTGT TTCTGACAAC ATGTTTGTTC ATAACAATTC TAAGCATGGA 
AGGAGAGCAA GAAGGCTTGA CCCATCTGAA GCTACACCCT GCATCAAAGC CATCAGCCCA 
AGTGAAGGCT GGACCACAGG AGGGGCCATG GTCATCATCA TTGGTGACAA CTTTTTTGAT 
GGTCTCCAGG TTGTGTTTGG TACCATGCTT GTATGGAGTG AGCTCATAAC CCCTCATGCC 
ATCAGAGTAC AGACCCCTCC CCGGCATATC CCTGGAGTTG TGGAAGTGAC ACTATCTTAT 
AAATCCAAAC AGTTCTGCAA AGGAGCCCCG GGCAGGTTCA TCTACACAGC CTTAAATGAA 
CCCACCATAG ACTATGGCTT CCAAAGATTG CAGAAAGTCA TCCCAAGGCA CCCAGGTGAC 
CCTGAGAGGC TAGCTAAGGA AATGCTGTTG AAAAGAGCTG CGGACCTGGT GGAAGCCCTC 
TACGGCACAC CACACAATAA TCAGGACATC ATTTTAAAGC GTGCTGCAGA CATTGCTGAA 
GCCCTGTACA GTGTCCCCAG GAACCCCAGC CAGATTCCAG CTCTTTCCAG CTCCCCAGCT 
CACAGCGGTA TGATGGGTAT CAACTCTTAC GGCAGCCAGC TCGGCGTCAG CATTTCAGAG 
TCCACACAAG GAAATAACCA AGGATACATC CGCAACACAA GCAGCATCTC CCCGCGGGGA 
TACTCCTCCA GCTCCACGCC TCAGCAATCT AATTACAGCA CCTCCAGCAA CAGCATGAAC 
GGCTACAGCA ATGTTCCTAT GGCCAACCTG GGTGTGCCAG GATCCCCGGG ATTTCTGAAT 
GGCTCACCCA CGGGCTCCCC ATATGGAATC ATGTCTTCAA GCCCCACCGT GGGATCCTCC 
AGCACGTCCT CCATCCTCCC GTTCTCCTCT TCAGTTTTTC CTGCTGTCAA ACAGAAGAGT 
GCCTTTGCCC CTGTCATCAG GCCCCAAGGC TCCCCATCGC CTGCCTGCTC CAGCGGCAAT 
GGGAACGGAT TCAGAGCCAT GACAGGACTT GTTGTTCCCC CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347131.1
CDS	492..2219
Translation	MFGIQDTLGRGPALKDKSLGAEMDSVRSWVRNVGVVDANVAAQSGVALSRAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVDFVENDKEQGNEKTNNGTHYKLQLLYSNGVRTEQDLYVRLIDSVTKQPIAYEGQNKNPEMCRVLLTHEVMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKTAGNPRDMRRFQVVLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGAMVIIIGDNFFDGLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLAKEMLLKRAADLVEALYGTPHNNQDIILKRAADIAEALYSVPRNPSQIPALSSSPAHSGMMGINSYGSQLGVSISESTQGNNQGYIRNTSSISPRGYSSSSTPQQSNYSTSSNSMNGYSNVPMANLGVPGSPGFLNGSPTGSPYGIMSSSPTVGSSSTSSILPFSSSVFPAVKQKSAFAPVIRPQGSPSPACSSGNGNGFRAMTGLVVPPM

Target ORF information:

RefSeq Version	NM_001360202.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus early B cell factor 2 (Ebf2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360202.1

ORF Insert Sequence:

ATGTTTGGGA TTCAAGATAC GCTAGGAAGA GGACCAGCTC TGAAAGACAA GTCGCTGGGT 
GCCGAGATGG ATTCGGTCAG GTCCTGGGTC CGGAATGTCG GCGTGGTGGA CGCCAATGTC 
GCGGCTCAGA GCGGAGTTGC TCTGTCCCGG GCCCATTTTG AAAAGCAACC TCCTTCAAAC 
TTGAGGAAAT CCAACTTCTT TCATTTTGTC CTGGCGCTCT ACGACAGGCA GGGACAGCCG 
GTGGAGATTG AGCGCACAGC CTTCGTGGAC TTTGTGGAGA ATGACAAAGA GCAAGGCAAT 
GAGAAGACCA ACAATGGTAC TCACTACAAG TTACAGCTCC TTTACAGCAA CGGTGTCCGC 
ACGGAGCAGG ACCTGTATGT CAGGCTCATC GACTCTGTCA CCAAACAGCC CATAGCCTAT 
GAGGGACAGA ATAAGAATCC CGAAATGTGC AGAGTTCTGC TGACACACGA GGTGATGTGC 
AGTCGATGCT GTGAGAAGAA GAGCTGCGGG AACCGGAACG AGACCCCTTC AGACCCGGTC 
ATCATCGACA GATTCTTTTT AAAATTTTTC CTGAAGTGCA ATCAGAATTG TTTGAAAACA 
GCGGGAAACC CAAGGGACAT GAGGCGGTTC CAGGTCGTGT TGTCCACAAC AGTAAATGTG 
GATGGACATG TGCTGGCTGT TTCTGACAAC ATGTTTGTTC ATAACAATTC TAAGCATGGA 
AGGAGAGCAA GAAGGCTTGA CCCATCTGAA GCTACACCCT GCATCAAAGC CATCAGCCCA 
AGTGAAGGCT GGACCACAGG AGGGGCCATG GTCATCATCA TTGGTGACAA CTTTTTTGAT 
GGTCTCCAGG TTGTGTTTGG TACCATGCTT GTATGGAGTG AGCTCATAAC CCCTCATGCC 
ATCAGAGTAC AGACCCCTCC CCGGCATATC CCTGGAGTTG TGGAAGTGAC ACTATCTTAT 
AAATCCAAAC AGTTCTGCAA AGGAGCCCCG GGCAGGTTCA TCTACACAGC CTTAAATGAA 
CCCACCATAG ACTATGGCTT CCAAAGATTG CAGAAAGTCA TCCCAAGGCA CCCAGGTGAC 
CCTGAGAGGC TAGCTAAGGA AATGCTGTTG AAAAGAGCTG CGGACCTGGT GGAAGCCCTC 
TACGGCACAC CACACAATAA TCAGGACATC ATTTTAAAGC GTGCTGCAGA CATTGCTGAA 
GCCCTGTACA GTGTCCCCAG GAACCCCAGC CAGATTCCAG CTCTTTCCAG CTCCCCAGCT 
CACAGCGGTA TGATGGGTAT CAACTCTTAC GGCAGCCAGC TCGGCGTCAG CATTTCAGAG 
TCCACACAAG GAAATAACCA AGGATACATC CGCAACACAA GCAGCATCTC CCCGCGGGGA 
TACTCCTCCA GCTCCACGCC TCAGCAATCT AATTACAGCA CCTCCAGCAA CAGCATGAAC 
GGCTACAGCA ATGTTCCTAT GGCCAACCTG GGTGTGCCAG GATCCCCGGG ATTTCTGAAT 
GGCTCACCCA CGGGCTCCCC ATATGGAATC ATGTCTTCAA GCCCCACCGT GGGATCCTCC 
AGCACGTCCT CCATCCTCCC GTTCTCCTCT TCAGTTTTTC CTGCTGTCAA ACAGAAGAGT 
GCCTTTGCCC CTGTCATCAG GCCCCAAGGC TCCCCATCGC CTGCCTGCTC CAGCGGCAAT 
GGGAACGGAT TCAGAGCCAT GACAGGACTT GTTGTTCCCC CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EBF2 ( XM_011244945.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011243247.1 Mus musculus early B cell factor 2 (Ebf2), transcript variant X1, mRNA.

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CloneID	OMu16972
Clone ID Related Accession (Same CDS sequence)	NM_010095.6 , XM_011244945.2 , NM_001276387.1 , NM_001360202.1 , XM_011244945.1
Accession Version	XM_011244945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transcription factor COE2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGGA TTCAAGATAC GCTAGGAAGA GGACCAGCTC TGAAAGACAA GTCGCTGGGT 
GCCGAGATGG ATTCGGTCAG GTCCTGGGTC CGGAATGTCG GCGTGGTGGA CGCCAATGTC 
GCGGCTCAGA GCGGAGTTGC TCTGTCCCGG GCCCATTTTG AAAAGCAACC TCCTTCAAAC 
TTGAGGAAAT CCAACTTCTT TCATTTTGTC CTGGCGCTCT ACGACAGGCA GGGACAGCCG 
GTGGAGATTG AGCGCACAGC CTTCGTGGAC TTTGTGGAGA ATGACAAAGA GCAAGGCAAT 
GAGAAGACCA ACAATGGTAC TCACTACAAG TTACAGCTCC TTTACAGCAA CGGTGTCCGC 
ACGGAGCAGG ACCTGTATGT CAGGCTCATC GACTCTGTCA CCAAACAGCC CATAGCCTAT 
GAGGGACAGA ATAAGAATCC CGAAATGTGC AGAGTTCTGC TGACACACGA GGTGATGTGC 
AGTCGATGCT GTGAGAAGAA GAGCTGCGGG AACCGGAACG AGACCCCTTC AGACCCGGTC 
ATCATCGACA GATTCTTTTT AAAATTTTTC CTGAAGTGCA ATCAGAATTG TTTGAAAACA 
GCGGGAAACC CAAGGGACAT GAGGCGGTTC CAGGTCGTGT TGTCCACAAC AGTAAATGTG 
GATGGACATG TGCTGGCTGT TTCTGACAAC ATGTTTGTTC ATAACAATTC TAAGCATGGA 
AGGAGAGCAA GAAGGCTTGA CCCATCTGAA GCTACACCCT GCATCAAAGC CATCAGCCCA 
AGTGAAGGCT GGACCACAGG AGGGGCCATG GTCATCATCA TTGGTGACAA CTTTTTTGAT 
GGTCTCCAGG TTGTGTTTGG TACCATGCTT GTATGGAGTG AGCTCATAAC CCCTCATGCC 
ATCAGAGTAC AGACCCCTCC CCGGCATATC CCTGGAGTTG TGGAAGTGAC ACTATCTTAT 
AAATCCAAAC AGTTCTGCAA AGGAGCCCCG GGCAGGTTCA TCTACACAGC CTTAAATGAA 
CCCACCATAG ACTATGGCTT CCAAAGATTG CAGAAAGTCA TCCCAAGGCA CCCAGGTGAC 
CCTGAGAGGC TAGCTAAGGA AATGCTGTTG AAAAGAGCTG CGGACCTGGT GGAAGCCCTC 
TACGGCACAC CACACAATAA TCAGGACATC ATTTTAAAGC GTGCTGCAGA CATTGCTGAA 
GCCCTGTACA GTGTCCCCAG GAACCCCAGC CAGATTCCAG CTCTTTCCAG CTCCCCAGCT 
CACAGCGGTA TGATGGGTAT CAACTCTTAC GGCAGCCAGC TCGGCGTCAG CATTTCAGAG 
TCCACACAAG GAAATAACCA AGGATACATC CGCAACACAA GCAGCATCTC CCCGCGGGGA 
TACTCCTCCA GCTCCACGCC TCAGCAATCT AATTACAGCA CCTCCAGCAA CAGCATGAAC 
GGCTACAGCA ATGTTCCTAT GGCCAACCTG GGTGTGCCAG GATCCCCGGG ATTTCTGAAT 
GGCTCACCCA CGGGCTCCCC ATATGGAATC ATGTCTTCAA GCCCCACCGT GGGATCCTCC 
AGCACGTCCT CCATCCTCCC GTTCTCCTCT TCAGTTTTTC CTGCTGTCAA ACAGAAGAGT 
GCCTTTGCCC CTGTCATCAG GCCCCAAGGC TCCCCATCGC CTGCCTGCTC CAGCGGCAAT 
GGGAACGGAT TCAGAGCCAT GACAGGACTT GTTGTTCCCC CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243247.1
CDS	536..2263
Translation	MFGIQDTLGRGPALKDKSLGAEMDSVRSWVRNVGVVDANVAAQSGVALSRAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVDFVENDKEQGNEKTNNGTHYKLQLLYSNGVRTEQDLYVRLIDSVTKQPIAYEGQNKNPEMCRVLLTHEVMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKTAGNPRDMRRFQVVLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGAMVIIIGDNFFDGLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLAKEMLLKRAADLVEALYGTPHNNQDIILKRAADIAEALYSVPRNPSQIPALSSSPAHSGMMGINSYGSQLGVSISESTQGNNQGYIRNTSSISPRGYSSSSTPQQSNYSTSSNSMNGYSNVPMANLGVPGSPGFLNGSPTGSPYGIMSSSPTVGSSSTSSILPFSSSVFPAVKQKSAFAPVIRPQGSPSPACSSGNGNGFRAMTGLVVPPM

Target ORF information:

RefSeq Version	XM_011244945.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus early B cell factor 2 (Ebf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244945.2

ORF Insert Sequence:

ATGTTTGGGA TTCAAGATAC GCTAGGAAGA GGACCAGCTC TGAAAGACAA GTCGCTGGGT 
GCCGAGATGG ATTCGGTCAG GTCCTGGGTC CGGAATGTCG GCGTGGTGGA CGCCAATGTC 
GCGGCTCAGA GCGGAGTTGC TCTGTCCCGG GCCCATTTTG AAAAGCAACC TCCTTCAAAC 
TTGAGGAAAT CCAACTTCTT TCATTTTGTC CTGGCGCTCT ACGACAGGCA GGGACAGCCG 
GTGGAGATTG AGCGCACAGC CTTCGTGGAC TTTGTGGAGA ATGACAAAGA GCAAGGCAAT 
GAGAAGACCA ACAATGGTAC TCACTACAAG TTACAGCTCC TTTACAGCAA CGGTGTCCGC 
ACGGAGCAGG ACCTGTATGT CAGGCTCATC GACTCTGTCA CCAAACAGCC CATAGCCTAT 
GAGGGACAGA ATAAGAATCC CGAAATGTGC AGAGTTCTGC TGACACACGA GGTGATGTGC 
AGTCGATGCT GTGAGAAGAA GAGCTGCGGG AACCGGAACG AGACCCCTTC AGACCCGGTC 
ATCATCGACA GATTCTTTTT AAAATTTTTC CTGAAGTGCA ATCAGAATTG TTTGAAAACA 
GCGGGAAACC CAAGGGACAT GAGGCGGTTC CAGGTCGTGT TGTCCACAAC AGTAAATGTG 
GATGGACATG TGCTGGCTGT TTCTGACAAC ATGTTTGTTC ATAACAATTC TAAGCATGGA 
AGGAGAGCAA GAAGGCTTGA CCCATCTGAA GCTACACCCT GCATCAAAGC CATCAGCCCA 
AGTGAAGGCT GGACCACAGG AGGGGCCATG GTCATCATCA TTGGTGACAA CTTTTTTGAT 
GGTCTCCAGG TTGTGTTTGG TACCATGCTT GTATGGAGTG AGCTCATAAC CCCTCATGCC 
ATCAGAGTAC AGACCCCTCC CCGGCATATC CCTGGAGTTG TGGAAGTGAC ACTATCTTAT 
AAATCCAAAC AGTTCTGCAA AGGAGCCCCG GGCAGGTTCA TCTACACAGC CTTAAATGAA 
CCCACCATAG ACTATGGCTT CCAAAGATTG CAGAAAGTCA TCCCAAGGCA CCCAGGTGAC 
CCTGAGAGGC TAGCTAAGGA AATGCTGTTG AAAAGAGCTG CGGACCTGGT GGAAGCCCTC 
TACGGCACAC CACACAATAA TCAGGACATC ATTTTAAAGC GTGCTGCAGA CATTGCTGAA 
GCCCTGTACA GTGTCCCCAG GAACCCCAGC CAGATTCCAG CTCTTTCCAG CTCCCCAGCT 
CACAGCGGTA TGATGGGTAT CAACTCTTAC GGCAGCCAGC TCGGCGTCAG CATTTCAGAG 
TCCACACAAG GAAATAACCA AGGATACATC CGCAACACAA GCAGCATCTC CCCGCGGGGA 
TACTCCTCCA GCTCCACGCC TCAGCAATCT AATTACAGCA CCTCCAGCAA CAGCATGAAC 
GGCTACAGCA ATGTTCCTAT GGCCAACCTG GGTGTGCCAG GATCCCCGGG ATTTCTGAAT 
GGCTCACCCA CGGGCTCCCC ATATGGAATC ATGTCTTCAA GCCCCACCGT GGGATCCTCC 
AGCACGTCCT CCATCCTCCC GTTCTCCTCT TCAGTTTTTC CTGCTGTCAA ACAGAAGAGT 
GCCTTTGCCC CTGTCATCAG GCCCCAAGGC TCCCCATCGC CTGCCTGCTC CAGCGGCAAT 
GGGAACGGAT TCAGAGCCAT GACAGGACTT GTTGTTCCCC CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EBF2 ( XM_011244945.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011243247.1 Mus musculus early B cell factor 2 (Ebf2), transcript variant X1, mRNA.

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CloneID	OMu16972
Clone ID Related Accession (Same CDS sequence)	NM_010095.6 , XM_011244945.2 , NM_001276387.1 , NM_001360202.1 , XM_011244945.1
Accession Version	XM_011244945.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	transcription factor COE2 isoform X1
Comment

ATGTTTGGGA TTCAAGATAC GCTAGGAAGA GGACCAGCTC TGAAAGACAA GTCGCTGGGT 
GCCGAGATGG ATTCGGTCAG GTCCTGGGTC CGGAATGTCG GCGTGGTGGA CGCCAATGTC 
GCGGCTCAGA GCGGAGTTGC TCTGTCCCGG GCCCATTTTG AAAAGCAACC TCCTTCAAAC 
TTGAGGAAAT CCAACTTCTT TCATTTTGTC CTGGCGCTCT ACGACAGGCA GGGACAGCCG 
GTGGAGATTG AGCGCACAGC CTTCGTGGAC TTTGTGGAGA ATGACAAAGA GCAAGGCAAT 
GAGAAGACCA ACAATGGTAC TCACTACAAG TTACAGCTCC TTTACAGCAA CGGTGTCCGC 
ACGGAGCAGG ACCTGTATGT CAGGCTCATC GACTCTGTCA CCAAACAGCC CATAGCCTAT 
GAGGGACAGA ATAAGAATCC CGAAATGTGC AGAGTTCTGC TGACACACGA GGTGATGTGC 
AGTCGATGCT GTGAGAAGAA GAGCTGCGGG AACCGGAACG AGACCCCTTC AGACCCGGTC 
ATCATCGACA GATTCTTTTT AAAATTTTTC CTGAAGTGCA ATCAGAATTG TTTGAAAACA 
GCGGGAAACC CAAGGGACAT GAGGCGGTTC CAGGTCGTGT TGTCCACAAC AGTAAATGTG 
GATGGACATG TGCTGGCTGT TTCTGACAAC ATGTTTGTTC ATAACAATTC TAAGCATGGA 
AGGAGAGCAA GAAGGCTTGA CCCATCTGAA GCTACACCCT GCATCAAAGC CATCAGCCCA 
AGTGAAGGCT GGACCACAGG AGGGGCCATG GTCATCATCA TTGGTGACAA CTTTTTTGAT 
GGTCTCCAGG TTGTGTTTGG TACCATGCTT GTATGGAGTG AGCTCATAAC CCCTCATGCC 
ATCAGAGTAC AGACCCCTCC CCGGCATATC CCTGGAGTTG TGGAAGTGAC ACTATCTTAT 
AAATCCAAAC AGTTCTGCAA AGGAGCCCCG GGCAGGTTCA TCTACACAGC CTTAAATGAA 
CCCACCATAG ACTATGGCTT CCAAAGATTG CAGAAAGTCA TCCCAAGGCA CCCAGGTGAC 
CCTGAGAGGC TAGCTAAGGA AATGCTGTTG AAAAGAGCTG CGGACCTGGT GGAAGCCCTC 
TACGGCACAC CACACAATAA TCAGGACATC ATTTTAAAGC GTGCTGCAGA CATTGCTGAA 
GCCCTGTACA GTGTCCCCAG GAACCCCAGC CAGATTCCAG CTCTTTCCAG CTCCCCAGCT 
CACAGCGGTA TGATGGGTAT CAACTCTTAC GGCAGCCAGC TCGGCGTCAG CATTTCAGAG 
TCCACACAAG GAAATAACCA AGGATACATC CGCAACACAA GCAGCATCTC CCCGCGGGGA 
TACTCCTCCA GCTCCACGCC TCAGCAATCT AATTACAGCA CCTCCAGCAA CAGCATGAAC 
GGCTACAGCA ATGTTCCTAT GGCCAACCTG GGTGTGCCAG GATCCCCGGG ATTTCTGAAT 
GGCTCACCCA CGGGCTCCCC ATATGGAATC ATGTCTTCAA GCCCCACCGT GGGATCCTCC 
AGCACGTCCT CCATCCTCCC GTTCTCCTCT TCAGTTTTTC CTGCTGTCAA ACAGAAGAGT 
GCCTTTGCCC CTGTCATCAG GCCCCAAGGC TCCCCATCGC CTGCCTGCTC CAGCGGCAAT 
GGGAACGGAT TCAGAGCCAT GACAGGACTT GTTGTTCCCC CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011243247.1

Translation

MFGIQDTLGRGPALKDKSLGAEMDSVRSWVRNVGVVDANVAAQSGVALSRAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVDFVENDKEQGNEKTNNGTHYKLQLLYSNGVRTEQDLYVRLIDSVTKQPIAYEGQNKNPEMCRVLLTHEVMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKTAGNPRDMRRFQVVLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGAMVIIIGDNFFDGLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLAKEMLLKRAADLVEALYGTPHNNQDIILKRAADIAEALYSVPRNPSQIPALSSSPAHSGMMGINSYGSQLGVSISESTQGNNQGYIRNTSSISPRGYSSSSTPQQSNYSTSSNSMNGYSNVPMANLGVPGSPGFLNGSPTGSPYGIMSSSPTVGSSSTSSILPFSSSVFPAVKQKSAFAPVIRPQGSPSPACSSGNGNGFRAMTGLVVPPM

Target ORF information:

RefSeq Version	XM_011244945.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus early B cell factor 2 (Ebf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244945.1

ORF Insert Sequence:

ATGTTTGGGA TTCAAGATAC GCTAGGAAGA GGACCAGCTC TGAAAGACAA GTCGCTGGGT 
GCCGAGATGG ATTCGGTCAG GTCCTGGGTC CGGAATGTCG GCGTGGTGGA CGCCAATGTC 
GCGGCTCAGA GCGGAGTTGC TCTGTCCCGG GCCCATTTTG AAAAGCAACC TCCTTCAAAC 
TTGAGGAAAT CCAACTTCTT TCATTTTGTC CTGGCGCTCT ACGACAGGCA GGGACAGCCG 
GTGGAGATTG AGCGCACAGC CTTCGTGGAC TTTGTGGAGA ATGACAAAGA GCAAGGCAAT 
GAGAAGACCA ACAATGGTAC TCACTACAAG TTACAGCTCC TTTACAGCAA CGGTGTCCGC 
ACGGAGCAGG ACCTGTATGT CAGGCTCATC GACTCTGTCA CCAAACAGCC CATAGCCTAT 
GAGGGACAGA ATAAGAATCC CGAAATGTGC AGAGTTCTGC TGACACACGA GGTGATGTGC 
AGTCGATGCT GTGAGAAGAA GAGCTGCGGG AACCGGAACG AGACCCCTTC AGACCCGGTC 
ATCATCGACA GATTCTTTTT AAAATTTTTC CTGAAGTGCA ATCAGAATTG TTTGAAAACA 
GCGGGAAACC CAAGGGACAT GAGGCGGTTC CAGGTCGTGT TGTCCACAAC AGTAAATGTG 
GATGGACATG TGCTGGCTGT TTCTGACAAC ATGTTTGTTC ATAACAATTC TAAGCATGGA 
AGGAGAGCAA GAAGGCTTGA CCCATCTGAA GCTACACCCT GCATCAAAGC CATCAGCCCA 
AGTGAAGGCT GGACCACAGG AGGGGCCATG GTCATCATCA TTGGTGACAA CTTTTTTGAT 
GGTCTCCAGG TTGTGTTTGG TACCATGCTT GTATGGAGTG AGCTCATAAC CCCTCATGCC 
ATCAGAGTAC AGACCCCTCC CCGGCATATC CCTGGAGTTG TGGAAGTGAC ACTATCTTAT 
AAATCCAAAC AGTTCTGCAA AGGAGCCCCG GGCAGGTTCA TCTACACAGC CTTAAATGAA 
CCCACCATAG ACTATGGCTT CCAAAGATTG CAGAAAGTCA TCCCAAGGCA CCCAGGTGAC 
CCTGAGAGGC TAGCTAAGGA AATGCTGTTG AAAAGAGCTG CGGACCTGGT GGAAGCCCTC 
TACGGCACAC CACACAATAA TCAGGACATC ATTTTAAAGC GTGCTGCAGA CATTGCTGAA 
GCCCTGTACA GTGTCCCCAG GAACCCCAGC CAGATTCCAG CTCTTTCCAG CTCCCCAGCT 
CACAGCGGTA TGATGGGTAT CAACTCTTAC GGCAGCCAGC TCGGCGTCAG CATTTCAGAG 
TCCACACAAG GAAATAACCA AGGATACATC CGCAACACAA GCAGCATCTC CCCGCGGGGA 
TACTCCTCCA GCTCCACGCC TCAGCAATCT AATTACAGCA CCTCCAGCAA CAGCATGAAC 
GGCTACAGCA ATGTTCCTAT GGCCAACCTG GGTGTGCCAG GATCCCCGGG ATTTCTGAAT 
GGCTCACCCA CGGGCTCCCC ATATGGAATC ATGTCTTCAA GCCCCACCGT GGGATCCTCC 
AGCACGTCCT CCATCCTCCC GTTCTCCTCT TCAGTTTTTC CTGCTGTCAA ACAGAAGAGT 
GCCTTTGCCC CTGTCATCAG GCCCCAAGGC TCCCCATCGC CTGCCTGCTC CAGCGGCAAT 
GGGAACGGAT TCAGAGCCAT GACAGGACTT GTTGTTCCCC CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Gastroenterology(2017 Oct)
Memic F,Knoflach V,Morarach K,Sadler R,Laranjeira C,Hjerling-Leffler J,Sundstr?m E,Pachnis V,Marklund U

EBF2 transcriptionally regulates brown adipogenesis via the histone reader DPF3 and the BAF chromatin remodeling complex.
Genes & development31(7)660-673(2017 Apr)
Shapira SN,Lim HW,Rajakumari S,Sakers AP,Ishibashi J,Harms MJ,Won KJ,Seale P

Comparative Transcriptomic and Epigenomic Analyses Reveal New Regulators of Murine Brown Adipogenesis.
PLoS genetics12(12)e1006474(2016 Dec)
Brunmeir R,Wu J,Peng X,Kim SY,Julien SG,Zhang Q,Xie W,Xu F

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EBF2 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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