Adam18 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adam18
Entrez Gene ID	13524
Full Name	a disintegrin and metallopeptidase domain 18
Synonyms	Adam27,Dtgn3,Tmdc3,tMDCIII
General protein information	Preferred Names a disintegrin and metallopeptidase domain 18 Names disintegrin and metalloproteinase domain-containing protein 18 disintegrin 3 disintegrin and metalloproteinase domain-containing protein 27 transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein III
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A2
Summary	This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. This gene is expressed in a regulated fashion during early stages of spermatogenesis. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. This gene is located in a cluster of related ADAM genes on chromosome 8. [provided by RefSeq, May 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010084.2	NP_034214.2	disintegrin and metalloproteinase domain-containing protein 18 preproprotein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Adam18	NP_034214.2
Canis lupus familiaris (dog)	ADAM18	XP_849584.3
Rattus norvegicus (Norway rat)	Adam18	XP_006253402.1
Homo sapiens (human)	ADAM18	NP_055052.1
Bos taurus (cattle)	ADAM18	XP_002698766.3
Pan troglodytes (chimpanzee)	ADAM18	XP_519721.4
	ADAM18	XP_002805364.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk MM
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)

Regulation of mesenchymal-to-epithelial transition by PARAXIS during somitogenesis.
Rowton M, Ramos P, Anderson DM, Rhee JM, Cunliffe HE, Rawls A
Developmental dynamics : an official publication of the American Association of Anatomists242(11)1332-44(2013 Nov)

Positive selection in the adhesion domain of Mus sperm Adam genes through gene duplications and function-driven gene complex formations.
Grayson P, Civetta A
BMC evolutionary biology13217(2013 Sep)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Girard A, Sachidanandam R, Hannon GJ, Carmell MA
Nature442(7099)199-202(2006 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Adam18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adam18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05481	NM_010084.2 Latest version!	Mus musculus a disintegrin and metallopeptidase domain 18 (Adam18), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adam18 ( NM_010084.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034214.2 Mus musculus a disintegrin and metallopeptidase domain 18 (Adam18), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05481
Clone ID Related Accession (Same CDS sequence)	NM_010084.2
Accession Version	NM_010084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	disintegrin and metalloproteinase domain-containing protein 18 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK076633.1, BY099176.1, AC138355.17 and AC114602.16. On Jun 30, 2009 this sequence version replaced NM_010084.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF167405.1, AK076633.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849381, SAMN00849383 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCTGC TCTTCATACT TGCGGAGCTT GCCATGCTGT TTGCCCGCCT CGATTCGGAA 
GGAATATGCC TGCATATCAC AGTGCCGCAG AAGATAGAGC CACGCAAGGG CGGAGATGCA 
GAAGGGAAGG TGACATACGT CATTACCATT GACGGGAAGC CCTACTCTCT GCATCTCAGG 
AACCACTCAT TTTTATCCCA GAACTTTTTG GTTTACACAT ACAATGAAAC TGGATCTTTG 
TATTCTGACT CATCACACTT TTTGGCTCAT TGCCATTACC GAGGATATGT TGATGAAGTG 
CCAAATTCCA TTGTGACACT CAGCATCTGC TCTGGCCTCA GGGGATTCCT GCAGTTGGAA 
AATGTCAGCT ATGGAATTGA ACCACTGGAG TCTTCAGCAA GGTTTGAGCA CATAGTTTAT 
CAAGTGAAGA GCGACAGCTC AATGCTAGCA GGAAATGACA GCCATGTTTG GCAGATAGAC 
CAGCTTGATA AAGGCCATTT CAATGAGCAG GATAAAAATC ATTCACAACT GTTACCTCAA 
AGTCTAAAAT TGCACATTAT AGTGGGGAAA TTTTTGTTTG ACTATATGGG ATCTGACATT 
ATGGCCATAA CACAGAAGAT TTTCCAGATT ATTGGTCTTG TTAACGCTAT GCTCACTCAG 
CTGAAATTGA GTGTTGTGTT AGCCTCCTTG GAATTGTGGT CAGATAAAAA TCATATTTCT 
ACTGACGGAA ATGCTACTGA TATTTTACAA AGACTTTTGG ACTGGAAACG AGACTATCTT 
ACCCTGCAGT CCAATGAAAT AACACACTTA CTCATTTACA GGAGACGCCC GAAATACATC 
GGAGCGGCAT CACCAGGTGA AATATGCAGT AAAAGCTATG TTGCGGGTGT TGGTATGTAT 
CCAGAAGACA TAGGTTTGGA GGGATTCTCA GTGGTTATCA CTCAACTGAT TGGCCTTCAC 
ATAGGATTAA CATACGACGA CAATATTAGA AATTGTTCCT GTCCCAGCGC TCCATGCATA 
ATGCAGCAGG GAGCACTGAG TTCCAGTGGG AAGAAGACTT TTAGCAACTG TAGCTTGCAT 
GACTACATGC ACTATGTTTC AAATTTTGAC ACGCAGTGTC TTGGTGACCT GTCAAATGTG 
CATGTATTGC AACCAAATCA AGCCGTGTGT GGCAATGGAA TCATGGAAGC TGGTGAGGAA 
TGTGACTGTG GTAATGAGAC GGAATGTCAA TTTAAAGAGT GCTGCGACCA TGAAACGTGC 
AGGCTAAAAG GCTCAGCACA ATGTGGATCT GGAGCTTGCT GCATGCCCAC GTGTGAGCTG 
TCAGCATCAG GCACGCCCTG TAGGAAAGCT GTTGACCCAG AGTGTGACTT CACAGAATAT 
TGCGATGGAT CCTCTAGCCA CTGTGTTCCT GACACCTTTG CACTGAACGG CCATTTGTGC 
AGGTTGGGAT CTGCATATTG CTACAATGGA AGATGCCAGG CTCTCAATGA CCAATGTGTC 
AGTTTATTTG GAAAAGGTTC TCAAGGGGCT TCCTATGCCT GTTTTGAAAA AGTGAATTCA 
CCACGTGAAA ATCTGGCAAA CTGTGATTCT AAAGACTCGT ACTCGGTACC TTGCGGACAG 
CAGGATGTTC TCTGTGGGAA ATTAGCTTGT TTTCGGCCGC CCAAGAATTA TAAGAGCCCT 
AGTCAATCTG TGGTCTATTC CTATGTCCAC GACAGCGTGT GTCTGTCCAT ACTTCCTGGG 
TTGTCTATGA GATCAGATGG CAGAGACAGC GCATACGTGG CTGACGGCAC TGTGTGTGGA 
CCACAAATGT ATTGTATAAA CGGAACCTGC AAAGAAGTTA ATTTCACAGG AAATGACTGC 
AACGCCACTA AGAAGTGCAA AGGGAATGGG ATATGTAATA ACTTTGGTAA TTGCCAATGC 
TTTCCTGACT ACAGGCCTCC AGATTGTAAC TTACAGATTG GATCACCGGG GGGCAGCATC 
GACGACGGGA ACACTCTCAG AACTGAGTCG GCTTTTGCTA CAAAGCGCCT GAGTAAGAAT 
GAGGACAGCT GGGTGATCCT GGGCTTCTTC ATCTTTCTGC CTTTCATCGT AACTTTCCTT 
GTTGGGATCA TGAAGAGAAA CGAAAGGAAA ATCGTGCCTC AGGGAGAACA CAAAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034214.2
CDS	59..2218
Translation	MPLLFILAELAMLFARLDSEGICLHITVPQKIEPRKGGDAEGKVTYVITIDGKPYSLHLRNHSFLSQNFLVYTYNETGSLYSDSSHFLAHCHYRGYVDEVPNSIVTLSICSGLRGFLQLENVSYGIEPLESSARFEHIVYQVKSDSSMLAGNDSHVWQIDQLDKGHFNEQDKNHSQLLPQSLKLHIIVGKFLFDYMGSDIMAITQKIFQIIGLVNAMLTQLKLSVVLASLELWSDKNHISTDGNATDILQRLLDWKRDYLTLQSNEITHLLIYRRRPKYIGAASPGEICSKSYVAGVGMYPEDIGLEGFSVVITQLIGLHIGLTYDDNIRNCSCPSAPCIMQQGALSSSGKKTFSNCSLHDYMHYVSNFDTQCLGDLSNVHVLQPNQAVCGNGIMEAGEECDCGNETECQFKECCDHETCRLKGSAQCGSGACCMPTCELSASGTPCRKAVDPECDFTEYCDGSSSHCVPDTFALNGHLCRLGSAYCYNGRCQALNDQCVSLFGKGSQGASYACFEKVNSPRENLANCDSKDSYSVPCGQQDVLCGKLACFRPPKNYKSPSQSVVYSYVHDSVCLSILPGLSMRSDGRDSAYVADGTVCGPQMYCINGTCKEVNFTGNDCNATKKCKGNGICNNFGNCQCFPDYRPPDCNLQIGSPGGSIDDGNTLRTESAFATKRLSKNEDSWVILGFFIFLPFIVTFLVGIMKRNERKIVPQGEHKI

Target ORF information:

RefSeq Version	NM_010084.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus a disintegrin and metallopeptidase domain 18 (Adam18), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010084.2

ORF Insert Sequence:

ATGCCTCTGC TCTTCATACT TGCGGAGCTT GCCATGCTGT TTGCCCGCCT CGATTCGGAA 
GGAATATGCC TGCATATCAC AGTGCCGCAG AAGATAGAGC CACGCAAGGG CGGAGATGCA 
GAAGGGAAGG TGACATACGT CATTACCATT GACGGGAAGC CCTACTCTCT GCATCTCAGG 
AACCACTCAT TTTTATCCCA GAACTTTTTG GTTTACACAT ACAATGAAAC TGGATCTTTG 
TATTCTGACT CATCACACTT TTTGGCTCAT TGCCATTACC GAGGATATGT TGATGAAGTG 
CCAAATTCCA TTGTGACACT CAGCATCTGC TCTGGCCTCA GGGGATTCCT GCAGTTGGAA 
AATGTCAGCT ATGGAATTGA ACCACTGGAG TCTTCAGCAA GGTTTGAGCA CATAGTTTAT 
CAAGTGAAGA GCGACAGCTC AATGCTAGCA GGAAATGACA GCCATGTTTG GCAGATAGAC 
CAGCTTGATA AAGGCCATTT CAATGAGCAG GATAAAAATC ATTCACAACT GTTACCTCAA 
AGTCTAAAAT TGCACATTAT AGTGGGGAAA TTTTTGTTTG ACTATATGGG ATCTGACATT 
ATGGCCATAA CACAGAAGAT TTTCCAGATT ATTGGTCTTG TTAACGCTAT GCTCACTCAG 
CTGAAATTGA GTGTTGTGTT AGCCTCCTTG GAATTGTGGT CAGATAAAAA TCATATTTCT 
ACTGACGGAA ATGCTACTGA TATTTTACAA AGACTTTTGG ACTGGAAACG AGACTATCTT 
ACCCTGCAGT CCAATGAAAT AACACACTTA CTCATTTACA GGAGACGCCC GAAATACATC 
GGAGCGGCAT CACCAGGTGA AATATGCAGT AAAAGCTATG TTGCGGGTGT TGGTATGTAT 
CCAGAAGACA TAGGTTTGGA GGGATTCTCA GTGGTTATCA CTCAACTGAT TGGCCTTCAC 
ATAGGATTAA CATACGACGA CAATATTAGA AATTGTTCCT GTCCCAGCGC TCCATGCATA 
ATGCAGCAGG GAGCACTGAG TTCCAGTGGG AAGAAGACTT TTAGCAACTG TAGCTTGCAT 
GACTACATGC ACTATGTTTC AAATTTTGAC ACGCAGTGTC TTGGTGACCT GTCAAATGTG 
CATGTATTGC AACCAAATCA AGCCGTGTGT GGCAATGGAA TCATGGAAGC TGGTGAGGAA 
TGTGACTGTG GTAATGAGAC GGAATGTCAA TTTAAAGAGT GCTGCGACCA TGAAACGTGC 
AGGCTAAAAG GCTCAGCACA ATGTGGATCT GGAGCTTGCT GCATGCCCAC GTGTGAGCTG 
TCAGCATCAG GCACGCCCTG TAGGAAAGCT GTTGACCCAG AGTGTGACTT CACAGAATAT 
TGCGATGGAT CCTCTAGCCA CTGTGTTCCT GACACCTTTG CACTGAACGG CCATTTGTGC 
AGGTTGGGAT CTGCATATTG CTACAATGGA AGATGCCAGG CTCTCAATGA CCAATGTGTC 
AGTTTATTTG GAAAAGGTTC TCAAGGGGCT TCCTATGCCT GTTTTGAAAA AGTGAATTCA 
CCACGTGAAA ATCTGGCAAA CTGTGATTCT AAAGACTCGT ACTCGGTACC TTGCGGACAG 
CAGGATGTTC TCTGTGGGAA ATTAGCTTGT TTTCGGCCGC CCAAGAATTA TAAGAGCCCT 
AGTCAATCTG TGGTCTATTC CTATGTCCAC GACAGCGTGT GTCTGTCCAT ACTTCCTGGG 
TTGTCTATGA GATCAGATGG CAGAGACAGC GCATACGTGG CTGACGGCAC TGTGTGTGGA 
CCACAAATGT ATTGTATAAA CGGAACCTGC AAAGAAGTTA ATTTCACAGG AAATGACTGC 
AACGCCACTA AGAAGTGCAA AGGGAATGGG ATATGTAATA ACTTTGGTAA TTGCCAATGC 
TTTCCTGACT ACAGGCCTCC AGATTGTAAC TTACAGATTG GATCACCGGG GGGCAGCATC 
GACGACGGGA ACACTCTCAG AACTGAGTCG GCTTTTGCTA CAAAGCGCCT GAGTAAGAAT 
GAGGACAGCT GGGTGATCCT GGGCTTCTTC ATCTTTCTGC CTTTCATCGT AACTTTCCTT 
GTTGGGATCA TGAAGAGAAA CGAAAGGAAA ATCGTGCCTC AGGGAGAACA CAAAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)
Miyata H,Castaneda JM,Fujihara Y,Yu Z,Archambeault DR,Isotani A,Kiyozumi D,Kriseman ML,Mashiko D,Matsumura T,Matzuk RM,Mori M,Noda T,Oji A,Okabe M,Prunskaite-Hyyrylainen R,Ramirez-Solis R,Satouh Y,Zhang Q,Ikawa M,Matzuk MM

Regulation of mesenchymal-to-epithelial transition by PARAXIS during somitogenesis.
Developmental dynamics : an official publication of the American Association of Anatomists242(11)1332-44(2013 Nov)
Rowton M,Ramos P,Anderson DM,Rhee JM,Cunliffe HE,Rawls A

Positive selection in the adhesion domain of Mus sperm Adam genes through gene duplications and function-driven gene complex formations.
BMC evolutionary biology13217(2013 Sep)
Grayson P,Civetta A

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Nature442(7099)199-202(2006 Jul)
Girard A,Sachidanandam R,Hannon GJ,Carmell MA

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Adam18 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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