Dmwd cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dmwd
Entrez Gene ID	13401
Full Name	dystrophia myotonica-containing WD repeat motif
Synonyms	DMR-N9,Dm9
General protein information	Preferred Names dystrophia myotonica-containing WD repeat motif Names dystrophia myotonica WD repeat-containing protein dystrophia myotonica linked gene, N9 dystrophia myotonica-containing WD repeat motif protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 A3\|7 9.46 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010058.2	NP_034188.2	dystrophia myotonica WD repeat-containing protein isoform 1
XM_006539524.4	XP_006539587.1	dystrophia myotonica WD repeat-containing protein isoform X1
NM_001374607.1	NP_001361536.1	dystrophia myotonica WD repeat-containing protein isoform 2
XM_006539524.3	XP_006539587.1	dystrophia myotonica WD repeat-containing protein isoform X1
XM_006539525.3	XP_006539588.1	dystrophia myotonica WD repeat-containing protein isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	DMWD	NP_004934.1
Pan troglodytes (chimpanzee)	DMWD	XP_001166545.1
Danio rerio (zebrafish)	LOC571996	XP_700740.5
Rattus norvegicus (Norway rat)	Dmwd	XP_006228596.1
Mus musculus (house mouse)	Dmwd	NP_034188.2
Canis lupus familiaris (dog)	DMWD	XP_541550.3
Bos taurus (cattle)	DMWD	XP_002695143.1

Gene Ontology
Bibliography

Related articles in PubMed

The DMWD protein from the myotonic dystrophy (DM1) gene region is developmentally regulated and is present most prominently in synapse-dense brain areas.
Westerlaken JH, Van der Zee CE, Peters W, Wieringa B
Brain research971(1)116-27(2003 May)

Transgenic mouse models for myotonic dystrophy type 1 (DM1).
Wansink DG, Wieringa B
Cytogenetic and genome research100(1-4)230-42(2003)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

New Dyscalc loci for myocardial cell necrosis and calcification (dystrophic cardiac calcinosis) in mice.
Ivandic BT, Utz HF, Kaczmarek PM, Aherrahrou Z, Axtner SB, Klepsch C, Lusis AJ, Katus HA
Physiological genomics6(3)137-44(2001 Aug)

Abnormal myotonic dystrophy protein kinase levels produce only mild myopathy in mice.
Jansen G, Groenen PJ, B?chner D, Jap PH, Coerwinkel M, Oerlemans F, van den Broek W, Gohlsch B, Pette D, Plomp JJ, Molenaar PC, Nederhoff MG, van Echteld CJ, Dekker M, Berns A, Hameister H, Wieringa B
Nature genetics13(3)316-24(1996 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The DMWD protein level gradually increased, was expressed specifically in the neuropil of areas with a high density of synaptic connections and was dispersed in a punctuate fashion throughout the neural cell body, but excluded from axons
Title: The DMWD protein from the myotonic dystrophy (DM1) gene region is developmentally regulated and is present most prominently in synapse-dense brain areas.

The following Dmwd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dmwd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu90614	NM_001374607.1 Latest version!	Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu37991	XM_006539524.4 Latest version!	Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu05443	NM_010058.2 Latest version!	Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu37992	XM_006539525.3 Latest version!	Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OMu37991	XM_006539524.3	Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dmwd ( NM_001374607.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001361536.1 Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu90614
Clone ID Related Accession (Same CDS sequence)	NM_001374607.1
Accession Version	NM_001374607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	dystrophia myotonica WD repeat-containing protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC170864.2. On Oct 7, 2019 this sequence version replaced XM_030242077.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.16187.1, SRR1660825.122543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG GCGGCGCGGA GGGCGGCCCG GGCCCCAGCG CCGCCATGGG CGACTGCGCG 
GAGATCAAGT CGCAGTTTCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGCG 
ACGCGCAGGT CGGGCCCGAC CTCCGCTCAG ACCCCGGCGC CGCCTCAGCC CACTCAGCCC 
CCGCCCGGGC CCGCCGCAGC CTCCGGGCCC GGCGCCGCGG GTCCCGCGTC GTCCCCGCCG 
CCCGCCGGCC CCGGGCCCGG GCCCGCGCTG CCCGCCGTCC GCCTCAGCCT GGTGCGCCTA 
GGCGACCCGG ATGGCGCTGG GGAGCCGCCC TCCACGCCCT CCGGACTGGG CGCGGGAGGA 
GACCGCGTCT GCTTCAACCT GGGCCGGGAG CTTTATTTCT ACCCCGGCTG CTGCCGCAGC 
GGGAGCCAGC GGTCCATCGA TCTCAACAAG CCCATCGACA AGCGGATCTA CAAGGGGACC 
CAGCCCACCT GCCACGACTT CAACCAGTTC ACAGCTGCCA CAGAGACCAT TTCCCTGCTG 
GTGGGCTTCT CTGCCGGCCA GGTGCAGTAT CTGGACCTCA TCAAGAAAGA CACCAGCAAG 
CTATTCAATG AAGAGCGGCT GATTGACAAG ACCAAGGTGA CATATTTGAA GTGGCTGCCT 
GAGTCAGAGA GTCTGTTCCT TGCTTCTCAC GCCAGTGGCC ACCTGTACCT CTACAACGTC 
AGTCACCCCT GCACCTCCAC CCCACCCCAG TATAGTCTGC TGAAACAGGG TGAGGGCTTT 
GCTGTCTATG CAGCCAAAAG CAAGGCACCC CGCAATCCAC TAGCCAAGTG GGCAGTGGGT 
GAGGGGCCCC TCAATGAGTT TGCATTCTCA CCTGATGGCC GACACTTAGC CTGTGTCAGC 
CAGGATGGCT GCCTGCGTGT CTTCCACTTT GACAGCATGC TTCTGCGTGG GCTCATGAAG 
AGCTACTTTG GGGGTCTGCT GTGTGTGTGC TGGAGCCCTG ATGGCCGCTA CGTGGTGACA 
GGAGGGGAAG ATGACTTAGT CACTGTGTGG TCCTTCACGG AGGGTCGTGT GGTGGCTCGA 
GGCCATGGCC ACAAGTCCTG GGTCAACGCT GTAGCCTTTG ATCCCTACAC CACTCGCGCA 
GAGGAGGCGG CGTCAGCCAG TGCTGATGGA GATCCCAGTG GTGAAGAGGA GGAGCCTGAA 
GTCACCAGCT CAGACACAGG AGCCCCAGTG TCCCCACTGC CCAAGGCTGG CTCCATCACG 
TACCGCTTTG GCTCAGCTGG CCAGGACACA CAGTTCTGCC TGTGGGACCT CACAGAAGAT 
GTGCTCTCCC CTCATCCGTC TCTGGCCCGT ACCCGCACCC TTCCGGGCAC ACCTGGTGCC 
ACCCCACCAG CTTCTGGTAG TTCTCGGGCC GGAGAGACAG GTGCAGGCCC CCTGCCCCGC 
TCCCTGTCTC GTTCCAACAG TCTCCCACAC CCAGCTGGTG GTGGCAAGGC TGGTGGGCCT 
AGTGCATCGA TGGAGCCTGG CATACCATTC AGCATTGGCC GCTTTGCCAC ACTGACCCTG 
CAGGAGCGGC GGGACCGGGG AGCTGAGAAG GAACACAAAC GCTACCATAG CCTGGGAAAC 
ATCAGCCGCG GTGGCAGTGG GGGCAATAGC AGCAATGACA AGCTCAGTGG TCCTGCCCCC 
CGAAGCCGAT TGGACCCAGC TAAGGTGCTG GGCACGGCAC TGTGCCCTCG GATCCATGAG 
GTGCCACTGC TGGAGCCTCT CGTGTGCAAG AAGATTGCTC AGGAACGCCT GACCGTGCTA 
CTGTTCCTGG AGGATTGTAT CATCACTGCC TGCCAAGAGG GCCTCATCTG CACCTGGGCC 
CGGCCAGGCA AGGCGGGCAT CTCCTCCCAA CCAGGCAGCT CCCCCAGTGG CACTGTGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361536.1
CDS	71..1993
Translation	MAAGGAEGGPGPSAAMGDCAEIKSQFRTREGFYKLLPGDATRRSGPTSAQTPAPPQPTQPPPGPAAASGPGAAGPASSPPPAGPGPGPALPAVRLSLVRLGDPDGAGEPPSTPSGLGAGGDRVCFNLGRELYFYPGCCRSGSQRSIDLNKPIDKRIYKGTQPTCHDFNQFTAATETISLLVGFSAGQVQYLDLIKKDTSKLFNEERLIDKTKVTYLKWLPESESLFLASHASGHLYLYNVSHPCTSTPPQYSLLKQGEGFAVYAAKSKAPRNPLAKWAVGEGPLNEFAFSPDGRHLACVSQDGCLRVFHFDSMLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAASASADGDPSGEEEEPEVTSSDTGAPVSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLSPHPSLARTRTLPGTPGATPPASGSSRAGETGAGPLPRSLSRSNSLPHPAGGGKAGGPSASMEPGIPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGNSSNDKLSGPAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAGISSQPGSSPSGTVV

Target ORF information:

RefSeq Version	NM_001374607.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374607.1

ORF Insert Sequence:

ATGGCGGCGG GCGGCGCGGA GGGCGGCCCG GGCCCCAGCG CCGCCATGGG CGACTGCGCG 
GAGATCAAGT CGCAGTTTCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGCG 
ACGCGCAGGT CGGGCCCGAC CTCCGCTCAG ACCCCGGCGC CGCCTCAGCC CACTCAGCCC 
CCGCCCGGGC CCGCCGCAGC CTCCGGGCCC GGCGCCGCGG GTCCCGCGTC GTCCCCGCCG 
CCCGCCGGCC CCGGGCCCGG GCCCGCGCTG CCCGCCGTCC GCCTCAGCCT GGTGCGCCTA 
GGCGACCCGG ATGGCGCTGG GGAGCCGCCC TCCACGCCCT CCGGACTGGG CGCGGGAGGA 
GACCGCGTCT GCTTCAACCT GGGCCGGGAG CTTTATTTCT ACCCCGGCTG CTGCCGCAGC 
GGGAGCCAGC GGTCCATCGA TCTCAACAAG CCCATCGACA AGCGGATCTA CAAGGGGACC 
CAGCCCACCT GCCACGACTT CAACCAGTTC ACAGCTGCCA CAGAGACCAT TTCCCTGCTG 
GTGGGCTTCT CTGCCGGCCA GGTGCAGTAT CTGGACCTCA TCAAGAAAGA CACCAGCAAG 
CTATTCAATG AAGAGCGGCT GATTGACAAG ACCAAGGTGA CATATTTGAA GTGGCTGCCT 
GAGTCAGAGA GTCTGTTCCT TGCTTCTCAC GCCAGTGGCC ACCTGTACCT CTACAACGTC 
AGTCACCCCT GCACCTCCAC CCCACCCCAG TATAGTCTGC TGAAACAGGG TGAGGGCTTT 
GCTGTCTATG CAGCCAAAAG CAAGGCACCC CGCAATCCAC TAGCCAAGTG GGCAGTGGGT 
GAGGGGCCCC TCAATGAGTT TGCATTCTCA CCTGATGGCC GACACTTAGC CTGTGTCAGC 
CAGGATGGCT GCCTGCGTGT CTTCCACTTT GACAGCATGC TTCTGCGTGG GCTCATGAAG 
AGCTACTTTG GGGGTCTGCT GTGTGTGTGC TGGAGCCCTG ATGGCCGCTA CGTGGTGACA 
GGAGGGGAAG ATGACTTAGT CACTGTGTGG TCCTTCACGG AGGGTCGTGT GGTGGCTCGA 
GGCCATGGCC ACAAGTCCTG GGTCAACGCT GTAGCCTTTG ATCCCTACAC CACTCGCGCA 
GAGGAGGCGG CGTCAGCCAG TGCTGATGGA GATCCCAGTG GTGAAGAGGA GGAGCCTGAA 
GTCACCAGCT CAGACACAGG AGCCCCAGTG TCCCCACTGC CCAAGGCTGG CTCCATCACG 
TACCGCTTTG GCTCAGCTGG CCAGGACACA CAGTTCTGCC TGTGGGACCT CACAGAAGAT 
GTGCTCTCCC CTCATCCGTC TCTGGCCCGT ACCCGCACCC TTCCGGGCAC ACCTGGTGCC 
ACCCCACCAG CTTCTGGTAG TTCTCGGGCC GGAGAGACAG GTGCAGGCCC CCTGCCCCGC 
TCCCTGTCTC GTTCCAACAG TCTCCCACAC CCAGCTGGTG GTGGCAAGGC TGGTGGGCCT 
AGTGCATCGA TGGAGCCTGG CATACCATTC AGCATTGGCC GCTTTGCCAC ACTGACCCTG 
CAGGAGCGGC GGGACCGGGG AGCTGAGAAG GAACACAAAC GCTACCATAG CCTGGGAAAC 
ATCAGCCGCG GTGGCAGTGG GGGCAATAGC AGCAATGACA AGCTCAGTGG TCCTGCCCCC 
CGAAGCCGAT TGGACCCAGC TAAGGTGCTG GGCACGGCAC TGTGCCCTCG GATCCATGAG 
GTGCCACTGC TGGAGCCTCT CGTGTGCAAG AAGATTGCTC AGGAACGCCT GACCGTGCTA 
CTGTTCCTGG AGGATTGTAT CATCACTGCC TGCCAAGAGG GCCTCATCTG CACCTGGGCC 
CGGCCAGGCA AGGCGGGCAT CTCCTCCCAA CCAGGCAGCT CCCCCAGTGG CACTGTGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dmwd ( XM_006539524.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539587.1 Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu37991
Clone ID Related Accession (Same CDS sequence)	XM_006539524.4 , XM_006539524.3
Accession Version	XM_006539524.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	dystrophia myotonica WD repeat-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539524.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GCGGCGCGGA GGGCGGCCCG GGCCCCAGCG CCGCCATGGG CGACTGCGCG 
GAGATCAAGT CGCAGTTTCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGCG 
ACGCGCAGGT CGGGCCCGAC CTCCGCTCAG ACCCCGGCGC CGCCTCAGCC CACTCAGCCC 
CCGCCCGGGC CCGCGCTGCC CGCCGTCCGC CTCAGCCTGG TGCGCCTAGG CGACCCGGAT 
GGCGCTGGGG AGCCGCCCTC CACGCCCTCC GGACTGGGCG CGGGAGGAGA CCGCGTCTGC 
TTCAACCTGG GCCGGGAGCT TTATTTCTAC CCCGGCTGCT GCCGCAGCGG GAGCCAGCGG 
TCCATCGATC TCAACAAGCC CATCGACAAG CGGATCTACA AGGGGACCCA GCCCACCTGC 
CACGACTTCA ACCAGTTCAC AGCTGCCACA GAGACCATTT CCCTGCTGGT GGGCTTCTCT 
GCCGGCCAGG TGCAGTATCT GGACCTCATC AAGAAAGACA CCAGCAAGCT ATTCAATGAA 
GAGCGGCTGA TTGACAAGAC CAAGGTGACA TATTTGAAGT GGCTGCCTGA GTCAGAGAGT 
CTGTTCCTTG CTTCTCACGC CAGTGGCCAC CTGTACCTCT ACAACGTCAG TCACCCCTGC 
ACCTCCACCC CACCCCAGTA TAGTCTGCTG AAACAGGGTG AGGGCTTTGC TGTCTATGCA 
GCCAAAAGCA AGGCACCCCG CAATCCACTA GCCAAGTGGG CAGTGGGTGA GGGGCCCCTC 
AATGAGTTTG CATTCTCACC TGATGGCCGA CACTTAGCCT GTGTCAGCCA GGATGGCTGC 
CTGCGTGTCT TCCACTTTGA CAGCATGCTT CTGCGTGGGC TCATGAAGAG CTACTTTGGG 
GGTCTGCTGT GTGTGTGCTG GAGCCCTGAT GGCCGCTACG TGGTGACAGG AGGGGAAGAT 
GACTTAGTCA CTGTGTGGTC CTTCACGGAG GGTCGTGTGG TGGCTCGAGG CCATGGCCAC 
AAGTCCTGGG TCAACGCTGT AGCCTTTGAT CCCTACACCA CTCGCGCAGA GGAGGCGGCG 
TCAGCCAGTG CTGATGGAGA TCCCAGTGGT GAAGAGGAGG AGCCTGAAGT CACCAGCTCA 
GACACAGGAG CCCCAGTGTC CCCACTGCCC AAGGCTGGCT CCATCACGTA CCGCTTTGGC 
TCAGCTGGCC AGGACACACA GTTCTGCCTG TGGGACCTCA CAGAAGATGT GCTCTCCCCT 
CATCCGTCTC TGGCCCGTAC CCGCACCCTT CCGGGCACAC CTGGTGCCAC CCCACCAGCT 
TCTGGTAGTT CTCGGGCCGG AGAGACAGGT GCAGGCCCCC TGCCCCGCTC CCTGTCTCGT 
TCCAACAGTC TCCCACACCC AGCTGGTGGT GGCAAGGCTG GTGGGCCTAG TGCATCGATG 
GAGCCTGGCA TACCATTCAG CATTGGCCGC TTTGCCACAC TGACCCTGCA GGAGCGGCGG 
GACCGGGGAG CTGAGAAGGA ACACAAACGC TACCATAGCC TGGGAAACAT CAGCCGCGGT 
GGCAGTGGGG GCAATAGCAG CAATGACAAG CTCAGTGGTC CTGCCCCCCG AAGCCGATTG 
GACCCAGCTA AGGTGCTGGG CACGGCACTG TGCCCTCGGA TCCATGAGGT GCCACTGCTG 
GAGCCTCTCG TGTGCAAGAA GATTGCTCAG GAACGCCTGA CCGTGCTACT GTTCCTGGAG 
GATTGTATCA TCACTGCCTG CCAAGAGGGC CTCATCTGCA CCTGGGCCCG GCCAGGCAAG 
GCGTTCACAG ACGAGGAGAC CGAGGCCCAG GCAGGGCAAG CAAGTTGGCC CAGGTCACCC 
AGCAAGTCAG TTGTAGAGGG CATCTCCTCC CAACCAGGCA GCTCCCCCAG TGGCACTGTG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539587.1
CDS	159..2084
Translation	MAAGGAEGGPGPSAAMGDCAEIKSQFRTREGFYKLLPGDATRRSGPTSAQTPAPPQPTQPPPGPALPAVRLSLVRLGDPDGAGEPPSTPSGLGAGGDRVCFNLGRELYFYPGCCRSGSQRSIDLNKPIDKRIYKGTQPTCHDFNQFTAATETISLLVGFSAGQVQYLDLIKKDTSKLFNEERLIDKTKVTYLKWLPESESLFLASHASGHLYLYNVSHPCTSTPPQYSLLKQGEGFAVYAAKSKAPRNPLAKWAVGEGPLNEFAFSPDGRHLACVSQDGCLRVFHFDSMLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAASASADGDPSGEEEEPEVTSSDTGAPVSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLSPHPSLARTRTLPGTPGATPPASGSSRAGETGAGPLPRSLSRSNSLPHPAGGGKAGGPSASMEPGIPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGNSSNDKLSGPAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAFTDEETEAQAGQASWPRSPSKSVVEGISSQPGSSPSGTVV

Target ORF information:

RefSeq Version	XM_006539524.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539524.4

ORF Insert Sequence:

ATGGCGGCGG GCGGCGCGGA GGGCGGCCCG GGCCCCAGCG CCGCCATGGG CGACTGCGCG 
GAGATCAAGT CGCAGTTTCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGCG 
ACGCGCAGGT CGGGCCCGAC CTCCGCTCAG ACCCCGGCGC CGCCTCAGCC CACTCAGCCC 
CCGCCCGGGC CCGCGCTGCC CGCCGTCCGC CTCAGCCTGG TGCGCCTAGG CGACCCGGAT 
GGCGCTGGGG AGCCGCCCTC CACGCCCTCC GGACTGGGCG CGGGAGGAGA CCGCGTCTGC 
TTCAACCTGG GCCGGGAGCT TTATTTCTAC CCCGGCTGCT GCCGCAGCGG GAGCCAGCGG 
TCCATCGATC TCAACAAGCC CATCGACAAG CGGATCTACA AGGGGACCCA GCCCACCTGC 
CACGACTTCA ACCAGTTCAC AGCTGCCACA GAGACCATTT CCCTGCTGGT GGGCTTCTCT 
GCCGGCCAGG TGCAGTATCT GGACCTCATC AAGAAAGACA CCAGCAAGCT ATTCAATGAA 
GAGCGGCTGA TTGACAAGAC CAAGGTGACA TATTTGAAGT GGCTGCCTGA GTCAGAGAGT 
CTGTTCCTTG CTTCTCACGC CAGTGGCCAC CTGTACCTCT ACAACGTCAG TCACCCCTGC 
ACCTCCACCC CACCCCAGTA TAGTCTGCTG AAACAGGGTG AGGGCTTTGC TGTCTATGCA 
GCCAAAAGCA AGGCACCCCG CAATCCACTA GCCAAGTGGG CAGTGGGTGA GGGGCCCCTC 
AATGAGTTTG CATTCTCACC TGATGGCCGA CACTTAGCCT GTGTCAGCCA GGATGGCTGC 
CTGCGTGTCT TCCACTTTGA CAGCATGCTT CTGCGTGGGC TCATGAAGAG CTACTTTGGG 
GGTCTGCTGT GTGTGTGCTG GAGCCCTGAT GGCCGCTACG TGGTGACAGG AGGGGAAGAT 
GACTTAGTCA CTGTGTGGTC CTTCACGGAG GGTCGTGTGG TGGCTCGAGG CCATGGCCAC 
AAGTCCTGGG TCAACGCTGT AGCCTTTGAT CCCTACACCA CTCGCGCAGA GGAGGCGGCG 
TCAGCCAGTG CTGATGGAGA TCCCAGTGGT GAAGAGGAGG AGCCTGAAGT CACCAGCTCA 
GACACAGGAG CCCCAGTGTC CCCACTGCCC AAGGCTGGCT CCATCACGTA CCGCTTTGGC 
TCAGCTGGCC AGGACACACA GTTCTGCCTG TGGGACCTCA CAGAAGATGT GCTCTCCCCT 
CATCCGTCTC TGGCCCGTAC CCGCACCCTT CCGGGCACAC CTGGTGCCAC CCCACCAGCT 
TCTGGTAGTT CTCGGGCCGG AGAGACAGGT GCAGGCCCCC TGCCCCGCTC CCTGTCTCGT 
TCCAACAGTC TCCCACACCC AGCTGGTGGT GGCAAGGCTG GTGGGCCTAG TGCATCGATG 
GAGCCTGGCA TACCATTCAG CATTGGCCGC TTTGCCACAC TGACCCTGCA GGAGCGGCGG 
GACCGGGGAG CTGAGAAGGA ACACAAACGC TACCATAGCC TGGGAAACAT CAGCCGCGGT 
GGCAGTGGGG GCAATAGCAG CAATGACAAG CTCAGTGGTC CTGCCCCCCG AAGCCGATTG 
GACCCAGCTA AGGTGCTGGG CACGGCACTG TGCCCTCGGA TCCATGAGGT GCCACTGCTG 
GAGCCTCTCG TGTGCAAGAA GATTGCTCAG GAACGCCTGA CCGTGCTACT GTTCCTGGAG 
GATTGTATCA TCACTGCCTG CCAAGAGGGC CTCATCTGCA CCTGGGCCCG GCCAGGCAAG 
GCGTTCACAG ACGAGGAGAC CGAGGCCCAG GCAGGGCAAG CAAGTTGGCC CAGGTCACCC 
AGCAAGTCAG TTGTAGAGGG CATCTCCTCC CAACCAGGCA GCTCCCCCAG TGGCACTGTG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dmwd ( NM_010058.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034188.2 Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05443
Clone ID Related Accession (Same CDS sequence)	NM_010058.2
Accession Version	NM_010058.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-06
Organism	Mus musculus(house mouse)
Product	dystrophia myotonica WD repeat-containing protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC170864.2, CF169673.1, BC089027.1 and AK086899.1. On Mar 25, 2009 this sequence version replaced NM_010058.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG GCGGCGCGGA GGGCGGCCCG GGCCCCAGCG CCGCCATGGG CGACTGCGCG 
GAGATCAAGT CGCAGTTTCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGCG 
ACGCGCAGGT CGGGCCCGAC CTCCGCTCAG ACCCCGGCGC CGCCTCAGCC CACTCAGCCC 
CCGCCCGGGC CCGCCGCAGC CTCCGGGCCC GGCGCCGCGG GTCCCGCGTC GTCCCCGCCG 
CCCGCCGGCC CCGGGCCCGG GCCCGCGCTG CCCGCCGTCC GCCTCAGCCT GGTGCGCCTA 
GGCGACCCGG ATGGCGCTGG GGAGCCGCCC TCCACGCCCT CCGGACTGGG CGCGGGAGGA 
GACCGCGTCT GCTTCAACCT GGGCCGGGAG CTTTATTTCT ACCCCGGCTG CTGCCGCAGC 
GGGAGCCAGC GGTCCATCGA TCTCAACAAG CCCATCGACA AGCGGATCTA CAAGGGGACC 
CAGCCCACCT GCCACGACTT CAACCAGTTC ACAGCTGCCA CAGAGACCAT TTCCCTGCTG 
GTGGGCTTCT CTGCCGGCCA GGTGCAGTAT CTGGACCTCA TCAAGAAAGA CACCAGCAAG 
CTATTCAATG AAGAGCGGCT GATTGACAAG ACCAAGGTGA CATATTTGAA GTGGCTGCCT 
GAGTCAGAGA GTCTGTTCCT TGCTTCTCAC GCCAGTGGCC ACCTGTACCT CTACAACGTC 
AGTCACCCCT GCACCTCCAC CCCACCCCAG TATAGTCTGC TGAAACAGGG TGAGGGCTTT 
GCTGTCTATG CAGCCAAAAG CAAGGCACCC CGCAATCCAC TAGCCAAGTG GGCAGTGGGT 
GAGGGGCCCC TCAATGAGTT TGCATTCTCA CCTGATGGCC GACACTTAGC CTGTGTCAGC 
CAGGATGGCT GCCTGCGTGT CTTCCACTTT GACAGCATGC TTCTGCGTGG GCTCATGAAG 
AGCTACTTTG GGGGTCTGCT GTGTGTGTGC TGGAGCCCTG ATGGCCGCTA CGTGGTGACA 
GGAGGGGAAG ATGACTTAGT CACTGTGTGG TCCTTCACGG AGGGTCGTGT GGTGGCTCGA 
GGCCATGGCC ACAAGTCCTG GGTCAACGCT GTAGCCTTTG ATCCCTACAC CACTCGCGCA 
GAGGAGGCGG CGTCAGCCAG TGCTGATGGA GATCCCAGTG GTGAAGAGGA GGAGCCTGAA 
GTCACCAGCT CAGACACAGG AGCCCCAGTG TCCCCACTGC CCAAGGCTGG CTCCATCACG 
TACCGCTTTG GCTCAGCTGG CCAGGACACA CAGTTCTGCC TGTGGGACCT CACAGAAGAT 
GTGCTCTCCC CTCATCCGTC TCTGGCCCGT ACCCGCACCC TTCCGGGCAC ACCTGGTGCC 
ACCCCACCAG CTTCTGGTAG TTCTCGGGCC GGAGAGACAG GTGCAGGCCC CCTGCCCCGC 
TCCCTGTCTC GTTCCAACAG TCTCCCACAC CCAGCTGGTG GTGGCAAGGC TGGTGGGCCT 
AGTGCATCGA TGGAGCCTGG CATACCATTC AGCATTGGCC GCTTTGCCAC ACTGACCCTG 
CAGGAGCGGC GGGACCGGGG AGCTGAGAAG GAACACAAAC GCTACCATAG CCTGGGAAAC 
ATCAGCCGCG GTGGCAGTGG GGGCAATAGC AGCAATGACA AGCTCAGTGG TCCTGCCCCC 
CGAAGCCGAT TGGACCCAGC TAAGGTGCTG GGCACGGCAC TGTGCCCTCG GATCCATGAG 
GTGCCACTGC TGGAGCCTCT CGTGTGCAAG AAGATTGCTC AGGAACGCCT GACCGTGCTA 
CTGTTCCTGG AGGATTGTAT CATCACTGCC TGCCAAGAGG GCCTCATCTG CACCTGGGCC 
CGGCCAGGCA AGGCGTTCAC AGACGAGGAG ACCGAGGCCC AGGCAGGGCA AGCAAGTTGG 
CCCAGGTCAC CCAGCAAGTC AGTTGTAGAG GGCATCTCCT CCCAACCAGG CAGCTCCCCC 
AGTGGCACTG TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034188.2
CDS	71..2068
Translation	MAAGGAEGGPGPSAAMGDCAEIKSQFRTREGFYKLLPGDATRRSGPTSAQTPAPPQPTQPPPGPAAASGPGAAGPASSPPPAGPGPGPALPAVRLSLVRLGDPDGAGEPPSTPSGLGAGGDRVCFNLGRELYFYPGCCRSGSQRSIDLNKPIDKRIYKGTQPTCHDFNQFTAATETISLLVGFSAGQVQYLDLIKKDTSKLFNEERLIDKTKVTYLKWLPESESLFLASHASGHLYLYNVSHPCTSTPPQYSLLKQGEGFAVYAAKSKAPRNPLAKWAVGEGPLNEFAFSPDGRHLACVSQDGCLRVFHFDSMLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAASASADGDPSGEEEEPEVTSSDTGAPVSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLSPHPSLARTRTLPGTPGATPPASGSSRAGETGAGPLPRSLSRSNSLPHPAGGGKAGGPSASMEPGIPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGNSSNDKLSGPAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAFTDEETEAQAGQASWPRSPSKSVVEGISSQPGSSPSGTVV

Target ORF information:

RefSeq Version	NM_010058.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010058.2

ORF Insert Sequence:

ATGGCGGCGG GCGGCGCGGA GGGCGGCCCG GGCCCCAGCG CCGCCATGGG CGACTGCGCG 
GAGATCAAGT CGCAGTTTCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGCG 
ACGCGCAGGT CGGGCCCGAC CTCCGCTCAG ACCCCGGCGC CGCCTCAGCC CACTCAGCCC 
CCGCCCGGGC CCGCCGCAGC CTCCGGGCCC GGCGCCGCGG GTCCCGCGTC GTCCCCGCCG 
CCCGCCGGCC CCGGGCCCGG GCCCGCGCTG CCCGCCGTCC GCCTCAGCCT GGTGCGCCTA 
GGCGACCCGG ATGGCGCTGG GGAGCCGCCC TCCACGCCCT CCGGACTGGG CGCGGGAGGA 
GACCGCGTCT GCTTCAACCT GGGCCGGGAG CTTTATTTCT ACCCCGGCTG CTGCCGCAGC 
GGGAGCCAGC GGTCCATCGA TCTCAACAAG CCCATCGACA AGCGGATCTA CAAGGGGACC 
CAGCCCACCT GCCACGACTT CAACCAGTTC ACAGCTGCCA CAGAGACCAT TTCCCTGCTG 
GTGGGCTTCT CTGCCGGCCA GGTGCAGTAT CTGGACCTCA TCAAGAAAGA CACCAGCAAG 
CTATTCAATG AAGAGCGGCT GATTGACAAG ACCAAGGTGA CATATTTGAA GTGGCTGCCT 
GAGTCAGAGA GTCTGTTCCT TGCTTCTCAC GCCAGTGGCC ACCTGTACCT CTACAACGTC 
AGTCACCCCT GCACCTCCAC CCCACCCCAG TATAGTCTGC TGAAACAGGG TGAGGGCTTT 
GCTGTCTATG CAGCCAAAAG CAAGGCACCC CGCAATCCAC TAGCCAAGTG GGCAGTGGGT 
GAGGGGCCCC TCAATGAGTT TGCATTCTCA CCTGATGGCC GACACTTAGC CTGTGTCAGC 
CAGGATGGCT GCCTGCGTGT CTTCCACTTT GACAGCATGC TTCTGCGTGG GCTCATGAAG 
AGCTACTTTG GGGGTCTGCT GTGTGTGTGC TGGAGCCCTG ATGGCCGCTA CGTGGTGACA 
GGAGGGGAAG ATGACTTAGT CACTGTGTGG TCCTTCACGG AGGGTCGTGT GGTGGCTCGA 
GGCCATGGCC ACAAGTCCTG GGTCAACGCT GTAGCCTTTG ATCCCTACAC CACTCGCGCA 
GAGGAGGCGG CGTCAGCCAG TGCTGATGGA GATCCCAGTG GTGAAGAGGA GGAGCCTGAA 
GTCACCAGCT CAGACACAGG AGCCCCAGTG TCCCCACTGC CCAAGGCTGG CTCCATCACG 
TACCGCTTTG GCTCAGCTGG CCAGGACACA CAGTTCTGCC TGTGGGACCT CACAGAAGAT 
GTGCTCTCCC CTCATCCGTC TCTGGCCCGT ACCCGCACCC TTCCGGGCAC ACCTGGTGCC 
ACCCCACCAG CTTCTGGTAG TTCTCGGGCC GGAGAGACAG GTGCAGGCCC CCTGCCCCGC 
TCCCTGTCTC GTTCCAACAG TCTCCCACAC CCAGCTGGTG GTGGCAAGGC TGGTGGGCCT 
AGTGCATCGA TGGAGCCTGG CATACCATTC AGCATTGGCC GCTTTGCCAC ACTGACCCTG 
CAGGAGCGGC GGGACCGGGG AGCTGAGAAG GAACACAAAC GCTACCATAG CCTGGGAAAC 
ATCAGCCGCG GTGGCAGTGG GGGCAATAGC AGCAATGACA AGCTCAGTGG TCCTGCCCCC 
CGAAGCCGAT TGGACCCAGC TAAGGTGCTG GGCACGGCAC TGTGCCCTCG GATCCATGAG 
GTGCCACTGC TGGAGCCTCT CGTGTGCAAG AAGATTGCTC AGGAACGCCT GACCGTGCTA 
CTGTTCCTGG AGGATTGTAT CATCACTGCC TGCCAAGAGG GCCTCATCTG CACCTGGGCC 
CGGCCAGGCA AGGCGTTCAC AGACGAGGAG ACCGAGGCCC AGGCAGGGCA AGCAAGTTGG 
CCCAGGTCAC CCAGCAAGTC AGTTGTAGAG GGCATCTCCT CCCAACCAGG CAGCTCCCCC 
AGTGGCACTG TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dmwd ( XM_006539524.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539587.1 Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant X1, mRNA.

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CloneID	OMu37991
Clone ID Related Accession (Same CDS sequence)	XM_006539524.4 , XM_006539524.3
Accession Version	XM_006539524.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	dystrophia myotonica WD repeat-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539524.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GCGGCGCGGA GGGCGGCCCG GGCCCCAGCG CCGCCATGGG CGACTGCGCG 
GAGATCAAGT CGCAGTTTCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGCG 
ACGCGCAGGT CGGGCCCGAC CTCCGCTCAG ACCCCGGCGC CGCCTCAGCC CACTCAGCCC 
CCGCCCGGGC CCGCGCTGCC CGCCGTCCGC CTCAGCCTGG TGCGCCTAGG CGACCCGGAT 
GGCGCTGGGG AGCCGCCCTC CACGCCCTCC GGACTGGGCG CGGGAGGAGA CCGCGTCTGC 
TTCAACCTGG GCCGGGAGCT TTATTTCTAC CCCGGCTGCT GCCGCAGCGG GAGCCAGCGG 
TCCATCGATC TCAACAAGCC CATCGACAAG CGGATCTACA AGGGGACCCA GCCCACCTGC 
CACGACTTCA ACCAGTTCAC AGCTGCCACA GAGACCATTT CCCTGCTGGT GGGCTTCTCT 
GCCGGCCAGG TGCAGTATCT GGACCTCATC AAGAAAGACA CCAGCAAGCT ATTCAATGAA 
GAGCGGCTGA TTGACAAGAC CAAGGTGACA TATTTGAAGT GGCTGCCTGA GTCAGAGAGT 
CTGTTCCTTG CTTCTCACGC CAGTGGCCAC CTGTACCTCT ACAACGTCAG TCACCCCTGC 
ACCTCCACCC CACCCCAGTA TAGTCTGCTG AAACAGGGTG AGGGCTTTGC TGTCTATGCA 
GCCAAAAGCA AGGCACCCCG CAATCCACTA GCCAAGTGGG CAGTGGGTGA GGGGCCCCTC 
AATGAGTTTG CATTCTCACC TGATGGCCGA CACTTAGCCT GTGTCAGCCA GGATGGCTGC 
CTGCGTGTCT TCCACTTTGA CAGCATGCTT CTGCGTGGGC TCATGAAGAG CTACTTTGGG 
GGTCTGCTGT GTGTGTGCTG GAGCCCTGAT GGCCGCTACG TGGTGACAGG AGGGGAAGAT 
GACTTAGTCA CTGTGTGGTC CTTCACGGAG GGTCGTGTGG TGGCTCGAGG CCATGGCCAC 
AAGTCCTGGG TCAACGCTGT AGCCTTTGAT CCCTACACCA CTCGCGCAGA GGAGGCGGCG 
TCAGCCAGTG CTGATGGAGA TCCCAGTGGT GAAGAGGAGG AGCCTGAAGT CACCAGCTCA 
GACACAGGAG CCCCAGTGTC CCCACTGCCC AAGGCTGGCT CCATCACGTA CCGCTTTGGC 
TCAGCTGGCC AGGACACACA GTTCTGCCTG TGGGACCTCA CAGAAGATGT GCTCTCCCCT 
CATCCGTCTC TGGCCCGTAC CCGCACCCTT CCGGGCACAC CTGGTGCCAC CCCACCAGCT 
TCTGGTAGTT CTCGGGCCGG AGAGACAGGT GCAGGCCCCC TGCCCCGCTC CCTGTCTCGT 
TCCAACAGTC TCCCACACCC AGCTGGTGGT GGCAAGGCTG GTGGGCCTAG TGCATCGATG 
GAGCCTGGCA TACCATTCAG CATTGGCCGC TTTGCCACAC TGACCCTGCA GGAGCGGCGG 
GACCGGGGAG CTGAGAAGGA ACACAAACGC TACCATAGCC TGGGAAACAT CAGCCGCGGT 
GGCAGTGGGG GCAATAGCAG CAATGACAAG CTCAGTGGTC CTGCCCCCCG AAGCCGATTG 
GACCCAGCTA AGGTGCTGGG CACGGCACTG TGCCCTCGGA TCCATGAGGT GCCACTGCTG 
GAGCCTCTCG TGTGCAAGAA GATTGCTCAG GAACGCCTGA CCGTGCTACT GTTCCTGGAG 
GATTGTATCA TCACTGCCTG CCAAGAGGGC CTCATCTGCA CCTGGGCCCG GCCAGGCAAG 
GCGTTCACAG ACGAGGAGAC CGAGGCCCAG GCAGGGCAAG CAAGTTGGCC CAGGTCACCC 
AGCAAGTCAG TTGTAGAGGG CATCTCCTCC CAACCAGGCA GCTCCCCCAG TGGCACTGTG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539587.1
CDS	181..2106
Translation	MAAGGAEGGPGPSAAMGDCAEIKSQFRTREGFYKLLPGDATRRSGPTSAQTPAPPQPTQPPPGPALPAVRLSLVRLGDPDGAGEPPSTPSGLGAGGDRVCFNLGRELYFYPGCCRSGSQRSIDLNKPIDKRIYKGTQPTCHDFNQFTAATETISLLVGFSAGQVQYLDLIKKDTSKLFNEERLIDKTKVTYLKWLPESESLFLASHASGHLYLYNVSHPCTSTPPQYSLLKQGEGFAVYAAKSKAPRNPLAKWAVGEGPLNEFAFSPDGRHLACVSQDGCLRVFHFDSMLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAASASADGDPSGEEEEPEVTSSDTGAPVSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLSPHPSLARTRTLPGTPGATPPASGSSRAGETGAGPLPRSLSRSNSLPHPAGGGKAGGPSASMEPGIPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGNSSNDKLSGPAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAFTDEETEAQAGQASWPRSPSKSVVEGISSQPGSSPSGTVV

Target ORF information:

RefSeq Version	XM_006539524.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539524.3

ORF Insert Sequence:

ATGGCGGCGG GCGGCGCGGA GGGCGGCCCG GGCCCCAGCG CCGCCATGGG CGACTGCGCG 
GAGATCAAGT CGCAGTTTCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGCG 
ACGCGCAGGT CGGGCCCGAC CTCCGCTCAG ACCCCGGCGC CGCCTCAGCC CACTCAGCCC 
CCGCCCGGGC CCGCGCTGCC CGCCGTCCGC CTCAGCCTGG TGCGCCTAGG CGACCCGGAT 
GGCGCTGGGG AGCCGCCCTC CACGCCCTCC GGACTGGGCG CGGGAGGAGA CCGCGTCTGC 
TTCAACCTGG GCCGGGAGCT TTATTTCTAC CCCGGCTGCT GCCGCAGCGG GAGCCAGCGG 
TCCATCGATC TCAACAAGCC CATCGACAAG CGGATCTACA AGGGGACCCA GCCCACCTGC 
CACGACTTCA ACCAGTTCAC AGCTGCCACA GAGACCATTT CCCTGCTGGT GGGCTTCTCT 
GCCGGCCAGG TGCAGTATCT GGACCTCATC AAGAAAGACA CCAGCAAGCT ATTCAATGAA 
GAGCGGCTGA TTGACAAGAC CAAGGTGACA TATTTGAAGT GGCTGCCTGA GTCAGAGAGT 
CTGTTCCTTG CTTCTCACGC CAGTGGCCAC CTGTACCTCT ACAACGTCAG TCACCCCTGC 
ACCTCCACCC CACCCCAGTA TAGTCTGCTG AAACAGGGTG AGGGCTTTGC TGTCTATGCA 
GCCAAAAGCA AGGCACCCCG CAATCCACTA GCCAAGTGGG CAGTGGGTGA GGGGCCCCTC 
AATGAGTTTG CATTCTCACC TGATGGCCGA CACTTAGCCT GTGTCAGCCA GGATGGCTGC 
CTGCGTGTCT TCCACTTTGA CAGCATGCTT CTGCGTGGGC TCATGAAGAG CTACTTTGGG 
GGTCTGCTGT GTGTGTGCTG GAGCCCTGAT GGCCGCTACG TGGTGACAGG AGGGGAAGAT 
GACTTAGTCA CTGTGTGGTC CTTCACGGAG GGTCGTGTGG TGGCTCGAGG CCATGGCCAC 
AAGTCCTGGG TCAACGCTGT AGCCTTTGAT CCCTACACCA CTCGCGCAGA GGAGGCGGCG 
TCAGCCAGTG CTGATGGAGA TCCCAGTGGT GAAGAGGAGG AGCCTGAAGT CACCAGCTCA 
GACACAGGAG CCCCAGTGTC CCCACTGCCC AAGGCTGGCT CCATCACGTA CCGCTTTGGC 
TCAGCTGGCC AGGACACACA GTTCTGCCTG TGGGACCTCA CAGAAGATGT GCTCTCCCCT 
CATCCGTCTC TGGCCCGTAC CCGCACCCTT CCGGGCACAC CTGGTGCCAC CCCACCAGCT 
TCTGGTAGTT CTCGGGCCGG AGAGACAGGT GCAGGCCCCC TGCCCCGCTC CCTGTCTCGT 
TCCAACAGTC TCCCACACCC AGCTGGTGGT GGCAAGGCTG GTGGGCCTAG TGCATCGATG 
GAGCCTGGCA TACCATTCAG CATTGGCCGC TTTGCCACAC TGACCCTGCA GGAGCGGCGG 
GACCGGGGAG CTGAGAAGGA ACACAAACGC TACCATAGCC TGGGAAACAT CAGCCGCGGT 
GGCAGTGGGG GCAATAGCAG CAATGACAAG CTCAGTGGTC CTGCCCCCCG AAGCCGATTG 
GACCCAGCTA AGGTGCTGGG CACGGCACTG TGCCCTCGGA TCCATGAGGT GCCACTGCTG 
GAGCCTCTCG TGTGCAAGAA GATTGCTCAG GAACGCCTGA CCGTGCTACT GTTCCTGGAG 
GATTGTATCA TCACTGCCTG CCAAGAGGGC CTCATCTGCA CCTGGGCCCG GCCAGGCAAG 
GCGTTCACAG ACGAGGAGAC CGAGGCCCAG GCAGGGCAAG CAAGTTGGCC CAGGTCACCC 
AGCAAGTCAG TTGTAGAGGG CATCTCCTCC CAACCAGGCA GCTCCCCCAG TGGCACTGTG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dmwd ( XM_006539525.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539588.1 Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu37992
Clone ID Related Accession (Same CDS sequence)	XM_006539525.3
Accession Version	XM_006539525.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	dystrophia myotonica WD repeat-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539525.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GCGGCGCGGA GGGCGGCCCG GGCCCCAGCG CCGCCATGGG CGACTGCGCG 
GAGATCAAGT CGCAGTTTCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGCG 
ACGCGCAGGT CGGGCCCGAC CTCCGCTCAG ACCCCGGCGC CGCCTCAGCC CACTCAGCCC 
CCGCCCGGGC CCGCGCTGCC CGCCGTCCGC CTCAGCCTGG TGCGCCTAGG CGACCCGGAT 
GGCGCTGGGG AGCCGCCCTC CACGCCCTCC GGACTGGGCG CGGGAGGAGA CCGCGTCTGC 
TTCAACCTGG GCCGGGAGCT TTATTTCTAC CCCGGCTGCT GCCGCAGCGG GAGCCAGCGG 
TCCATCGATC TCAACAAGCC CATCGACAAG CGGATCTACA AGGGGACCCA GCCCACCTGC 
CACGACTTCA ACCAGTTCAC AGCTGCCACA GAGACCATTT CCCTGCTGGT GGGCTTCTCT 
GCCGGCCAGG TGCAGTATCT GGACCTCATC AAGAAAGACA CCAGCAAGCT ATTCAATGAA 
GAGCGGCTGA TTGACAAGAC CAAGGTGACA TATTTGAAGT GGCTGCCTGA GTCAGAGAGT 
CTGTTCCTTG CTTCTCACGC CAGTGGCCAC CTGTACCTCT ACAACGTCAG TCACCCCTGC 
ACCTCCACCC CACCCCAGTA TAGTCTGCTG AAACAGGGTG AGGGCTTTGC TGTCTATGCA 
GCCAAAAGCA AGGCACCCCG CAATCCACTA GCCAAGTGGG CAGTGGGTGA GGGGCCCCTC 
AATGAGTTTG CATTCTCACC TGATGGCCGA CACTTAGCCT GTGTCAGCCA GGATGGCTGC 
CTGCGTGTCT TCCACTTTGA CAGCATGCTT CTGCGTGGGC TCATGAAGAG CTACTTTGGG 
GGTCTGCTGT GTGTGTGCTG GAGCCCTGAT GGCCGCTACG TGGTGACAGG AGGGGAAGAT 
GACTTAGTCA CTGTGTGGTC CTTCACGGAG GGTCGTGTGG TGGCTCGAGG CCATGGCCAC 
AAGTCCTGGG TCAACGCTGT AGCCTTTGAT CCCTACACCA CTCGCGCAGA GGAGGCGGCG 
TCAGCCAGTG CTGATGGAGA TCCCAGTGGT GAAGAGGAGG AGCCTGAAGT CACCAGCTCA 
GACACAGGAG CCCCAGTGTC CCCACTGCCC AAGGCTGGCT CCATCACGTA CCGCTTTGGC 
TCAGCTGGCC AGGACACACA GTTCTGCCTG TGGGACCTCA CAGAAGATGT GCTCTCCCCT 
CATCCGTCTC TGGCCCGTAC CCGCACCCTT CCGGGCACAC CTGGTGCCAC CCCACCAGCT 
TCTGGTAGTT CTCGGGCCGG AGAGACAGGT GCAGGCCCCC TGCCCCGCTC CCTGTCTCGT 
TCCAACAGTC TCCCACACCC AGCTGGTGGT GGCAAGGCTG GTGGGCCTAG TGCATCGATG 
GAGCCTGGCA TACCATTCAG CATTGGCCGC TTTGCCACAC TGACCCTGCA GGAGCGGCGG 
GACCGGGGAG CTGAGAAGGA ACACAAACGC TACCATAGCC TGGGAAACAT CAGCCGCGGT 
GGCAGTGGGG GCAATAGCAG CAATGACAAG CTCAGTGGTC CTGCCCCCCG AAGCCGATTG 
GACCCAGCTA AGGTGCTGGG CACGGCACTG TGCCCTCGGA TCCATGAGGT GCCACTGCTG 
GAGCCTCTCG TGTGCAAGAA GATTGCTCAG GAACGCCTGA CCGTGCTACT GTTCCTGGAG 
GATTGTATCA TCACTGCCTG CCAAGAGGGC CTCATCTGCA CCTGGGCCCG GCCAGGCAAG 
GCGGGCATCT CCTCCCAACC AGGCAGCTCC CCCAGTGGCA CTGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539588.1
CDS	180..2030
Translation	MAAGGAEGGPGPSAAMGDCAEIKSQFRTREGFYKLLPGDATRRSGPTSAQTPAPPQPTQPPPGPALPAVRLSLVRLGDPDGAGEPPSTPSGLGAGGDRVCFNLGRELYFYPGCCRSGSQRSIDLNKPIDKRIYKGTQPTCHDFNQFTAATETISLLVGFSAGQVQYLDLIKKDTSKLFNEERLIDKTKVTYLKWLPESESLFLASHASGHLYLYNVSHPCTSTPPQYSLLKQGEGFAVYAAKSKAPRNPLAKWAVGEGPLNEFAFSPDGRHLACVSQDGCLRVFHFDSMLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAASASADGDPSGEEEEPEVTSSDTGAPVSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLSPHPSLARTRTLPGTPGATPPASGSSRAGETGAGPLPRSLSRSNSLPHPAGGGKAGGPSASMEPGIPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGNSSNDKLSGPAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAGISSQPGSSPSGTVV

Target ORF information:

RefSeq Version	XM_006539525.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dystrophia myotonica-containing WD repeat motif (Dmwd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539525.3

ORF Insert Sequence:

ATGGCGGCGG GCGGCGCGGA GGGCGGCCCG GGCCCCAGCG CCGCCATGGG CGACTGCGCG 
GAGATCAAGT CGCAGTTTCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGCG 
ACGCGCAGGT CGGGCCCGAC CTCCGCTCAG ACCCCGGCGC CGCCTCAGCC CACTCAGCCC 
CCGCCCGGGC CCGCGCTGCC CGCCGTCCGC CTCAGCCTGG TGCGCCTAGG CGACCCGGAT 
GGCGCTGGGG AGCCGCCCTC CACGCCCTCC GGACTGGGCG CGGGAGGAGA CCGCGTCTGC 
TTCAACCTGG GCCGGGAGCT TTATTTCTAC CCCGGCTGCT GCCGCAGCGG GAGCCAGCGG 
TCCATCGATC TCAACAAGCC CATCGACAAG CGGATCTACA AGGGGACCCA GCCCACCTGC 
CACGACTTCA ACCAGTTCAC AGCTGCCACA GAGACCATTT CCCTGCTGGT GGGCTTCTCT 
GCCGGCCAGG TGCAGTATCT GGACCTCATC AAGAAAGACA CCAGCAAGCT ATTCAATGAA 
GAGCGGCTGA TTGACAAGAC CAAGGTGACA TATTTGAAGT GGCTGCCTGA GTCAGAGAGT 
CTGTTCCTTG CTTCTCACGC CAGTGGCCAC CTGTACCTCT ACAACGTCAG TCACCCCTGC 
ACCTCCACCC CACCCCAGTA TAGTCTGCTG AAACAGGGTG AGGGCTTTGC TGTCTATGCA 
GCCAAAAGCA AGGCACCCCG CAATCCACTA GCCAAGTGGG CAGTGGGTGA GGGGCCCCTC 
AATGAGTTTG CATTCTCACC TGATGGCCGA CACTTAGCCT GTGTCAGCCA GGATGGCTGC 
CTGCGTGTCT TCCACTTTGA CAGCATGCTT CTGCGTGGGC TCATGAAGAG CTACTTTGGG 
GGTCTGCTGT GTGTGTGCTG GAGCCCTGAT GGCCGCTACG TGGTGACAGG AGGGGAAGAT 
GACTTAGTCA CTGTGTGGTC CTTCACGGAG GGTCGTGTGG TGGCTCGAGG CCATGGCCAC 
AAGTCCTGGG TCAACGCTGT AGCCTTTGAT CCCTACACCA CTCGCGCAGA GGAGGCGGCG 
TCAGCCAGTG CTGATGGAGA TCCCAGTGGT GAAGAGGAGG AGCCTGAAGT CACCAGCTCA 
GACACAGGAG CCCCAGTGTC CCCACTGCCC AAGGCTGGCT CCATCACGTA CCGCTTTGGC 
TCAGCTGGCC AGGACACACA GTTCTGCCTG TGGGACCTCA CAGAAGATGT GCTCTCCCCT 
CATCCGTCTC TGGCCCGTAC CCGCACCCTT CCGGGCACAC CTGGTGCCAC CCCACCAGCT 
TCTGGTAGTT CTCGGGCCGG AGAGACAGGT GCAGGCCCCC TGCCCCGCTC CCTGTCTCGT 
TCCAACAGTC TCCCACACCC AGCTGGTGGT GGCAAGGCTG GTGGGCCTAG TGCATCGATG 
GAGCCTGGCA TACCATTCAG CATTGGCCGC TTTGCCACAC TGACCCTGCA GGAGCGGCGG 
GACCGGGGAG CTGAGAAGGA ACACAAACGC TACCATAGCC TGGGAAACAT CAGCCGCGGT 
GGCAGTGGGG GCAATAGCAG CAATGACAAG CTCAGTGGTC CTGCCCCCCG AAGCCGATTG 
GACCCAGCTA AGGTGCTGGG CACGGCACTG TGCCCTCGGA TCCATGAGGT GCCACTGCTG 
GAGCCTCTCG TGTGCAAGAA GATTGCTCAG GAACGCCTGA CCGTGCTACT GTTCCTGGAG 
GATTGTATCA TCACTGCCTG CCAAGAGGGC CTCATCTGCA CCTGGGCCCG GCCAGGCAAG 
GCGGGCATCT CCTCCCAACC AGGCAGCTCC CCCAGTGGCA CTGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The DMWD protein from the myotonic dystrophy (DM1) gene region is developmentally regulated and is present most prominently in synapse-dense brain areas.
Brain research971(1)116-27(2003 May)
Westerlaken JH,Van der Zee CE,Peters W,Wieringa B

Transgenic mouse models for myotonic dystrophy type 1 (DM1).
Cytogenetic and genome research100(1-4)230-42(2003)
Wansink DG,Wieringa B

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS

New Dyscalc loci for myocardial cell necrosis and calcification (dystrophic cardiac calcinosis) in mice.
Physiological genomics6(3)137-44(2001 Aug)
Ivandic BT,Utz HF,Kaczmarek PM,Aherrahrou Z,Axtner SB,Klepsch C,Lusis AJ,Katus HA

Abnormal myotonic dystrophy protein kinase levels produce only mild myopathy in mice.
Nature genetics13(3)316-24(1996 Jul)
Jansen G,Groenen PJ,B?chner D,Jap PH,Coerwinkel M,Oerlemans F,van den Broek W,Gohlsch B,Pette D,Plomp JJ,Molenaar PC,Nederhoff MG,van Echteld CJ,Dekker M,Berns A,Hameister H,Wieringa B

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Dmwd cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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