Dld cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dld
Entrez Gene ID	13382
Full Name	dihydrolipoamide dehydrogenase
General protein information	Preferred Names dihydrolipoamide dehydrogenase Names dihydrolipoyl dehydrogenase, mitochondrial branched chain alpha-keto acid dehydrogenase complex subunit E3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 A2\|12 13.43 cM
Summary	This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. [provided by RefSeq, Jan 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007861.5	NP_031887.2	dihydrolipoyl dehydrogenase, mitochondrial precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dld	NP_955417.1
Caenorhabditis elegans (roundworm)	dld-1	NP_001255810.1
Neurospora crassa	NCU02407	XP_959535.2
Arabidopsis thaliana (thale cress)	mtLPD2	NP_566570.3
Homo sapiens (human)	DLD	NP_000099.2
	DLD	XP_001095232.2
Canis lupus familiaris (dog)	DLD	NP_001003294.1
Mus musculus (house mouse)	Dld	NP_031887.2
Danio rerio (zebrafish)	dldh	NP_958914.1
Magnaporthe oryzae (rice blast fungus)	MGG_17072	XP_003716343.1
Pan troglodytes (chimpanzee)	DLD	XP_001165051.2
Gallus gallus (chicken)	DLD	NP_001025898.1
Drosophila melanogaster (fruit fly)	CG7430	NP_649017.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011629	XP_320877.4
Kluyveromyces lactis	KLLA0D11154g	XP_453559.1
Eremothecium gossypii	AGOS_AFR512W	NP_986059.2
Oryza sativa (rice)	Os01g0328700	NP_001042918.1
Bos taurus (cattle)	DLD	NP_001193099.1
Saccharomyces cerevisiae (baker's yeast)	LPD1	NP_116635.1
Schizosaccharomyces pombe (fission yeast)	dld1	NP_593496.1
Arabidopsis thaliana (thale cress)	mtLPD1	NP_175237.1
Xenopus tropicalis (tropical clawed frog)	dld	XP_002933161.2

Gene Ontology
Bibliography

Related articles in PubMed

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling.
Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson J, Babu M
Cell systems5(6)564-577.e12(2017 Dec)

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

Dihydrolipoyl dehydrogenase as a potential UVB target in skin epidermis; using an integrated approach of label-free quantitative proteomics and targeted metabolite analysis.
Moon E, Park HM, Lee CH, Do SG, Park JM, Han NY, Do MH, Lee JH, Lee H, Kim SY
Journal of proteomics11770-85(2015 Mar)

Mitochondrial Dihydrolipoamide Dehydrogenase is Upregulated in Response to Intermittent Hypoxic Preconditioning.
Li R, Luo X, Wu J, Thangthaeng N, Jung ME, Jing S, Li L, Ellis DZ, Liu L, Ding Z, Forster MJ, Yan LJ
International journal of medical sciences12(5)432-40(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DLD, malic acid, and fumaric acid can be used for development of cosmeceuticals and nutraceuticals regulating the change of skin metabolism induced by the UVB overexposure.
Title: Dihydrolipoyl dehydrogenase as a potential UVB target in skin epidermis; using an integrated approach of label-free quantitative proteomics and targeted metabolite analysis.

Mitochondrial dihydrolipoamide dehydrogenase is upregulated in response to the brain intermittent hypoxic preconditioning.
Title: Mitochondrial Dihydrolipoamide Dehydrogenase is Upregulated in Response to Intermittent Hypoxic Preconditioning.

Data show that mitochondrial diaphorases (dihydrolipoamide dehydrogenase) in the liver contribute up to 81% to the NAD(+) pool during respiratory inhibition.
Title: Mitochondrial diaphorases as NAD? donors to segments of the citric acid cycle that support substrate-level phosphorylation yielding ATP during respiratory inhibition.

ATP consumption is demonstrated in respiration-impaired isolated mice and in situ neuronal mitochondria from transgenic mice with dihydrolipoyl succinyltransferase deficiency.
Title: The negative impact of ?-ketoglutarate dehydrogenase complex deficiency on matrix substrate-level phosphorylation.

Human, mouse, and pig Dld has moonlighting function as a protease in addition to its canonical function as a a dehydrogenase.
Title: Cryptic proteolytic activity of dihydrolipoamide dehydrogenase.

The following Dld gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dld cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16568	NM_007861.5 Latest version!	Mus musculus dihydrolipoamide dehydrogenase (Dld), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dld ( NM_007861.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031887.2 Mus musculus dihydrolipoamide dehydrogenase (Dld), mRNA.

Price & Availability↑

CloneID	OMu16568
Clone ID Related Accession (Same CDS sequence)	NM_007861.5
Accession Version	NM_007861.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Mus musculus(house mouse)
Product	dihydrolipoyl dehydrogenase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY282701.1, AK117104.1 and AI849462.1. On Jan 24, 2014 this sequence version replaced NM_007861.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.142418.1, SRR1660815.257761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta multifunctional gene product(s) :: PMID: 17404228 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGCT GGAGTCGTGT GTACCGCTCC TTGGCCAAGA AAGGCCATTT CAATCGGATA 
TCTCATGGCC TACAAGGAGT TTCTTCAGTT CCTTTAAGAA CTTATGCAGA TCAACCAATT 
GAGGCTGACG TGACAGTGAT AGGTTCTGGC CCTGGAGGAT ATGTTGCTGC CATCAAATCT 
GCCCAGTTAG GCTTTAAGAC AGTCTGCATT GAGAAAAATG AGACACTAGG TGGAACATGC 
TTGAACGTTG GTTGTATTCC TTCAAAGGCT TTATTAAATA ATTCTCATTA TTACCATATG 
GCCCACGGAA AAGATTTTGC ATCTAGGGGA ATTGAAATAC CAGAAGTTCG CTTGAATTTA 
GAGAAGATGA TGGAGCAGAA GCATTCTGCA GTAAAAGCAT TAACAGGGGG AATTGCTCAC 
TTATTCAAAC AAAATAAGGT TGTTCACGTC AATGGATTTG GAAAGATAAC CGGCAAGAAT 
CAAGTTACAG CTACAAAAGC CGATGGCAGC ACTCAGGTTA TTGATACCAA AAACATCCTT 
GTAGCTACGG GCTCAGAAGT TACTCCTTTT CCTGGAATCA CGATCGATGA AGATACTATA 
GTGTCATCTA CAGGAGCTTT ATCCTTAAAA AAAGTTCCAG AGAAGCTGGT TGTTATTGGG 
GCAGGAGTAA TTGGTGTGGA ACTGGGTTCA GTTTGGCAAA GACTTGGTGC AGATGTGACA 
GCAGTTGAAT TTTTGGGTCA TGTGGGTGGA ATTGGAATTG ACATGGAGAT ATCAAAAAAT 
TTTCAACGTA TACTTCAGAG GCAAGGATTT AAGTTTAAAC TGAATACAAA AGTTACTGGT 
GCCACCAAGA AGTCAGATGG AAAAATTGAT GTGTCTGTCG AAGCTGCTTC TGGTGGTAAA 
GCTGAAGTTA TCACTTGTGA TGTACTCTTG GTTTGCATTG GTCGGCGGCC CTTTACTCAG 
AATTTGGGAC TAGAAGAGCT TGGAATTGAA CTAGATCCCA AAGGTAGAAT TCCAGTCAAT 
AACAGATTTC AAACTAAAAT TCCAAATATC TATGCTATTG GAGATGTGGT CGCTGGTCCA 
ATGTTGGCTC ACAAAGCAGA AGATGAAGGC ATCATCTGTG TTGAAGGGAT GGCCGGCGGT 
GCTGTGCACA TTGATTATAA TTGTGTGCCA TCAGTGATTT ACACACACCC CGAGGTTGCT 
TGGGTTGGCA AATCGGAAGA ACAGTTGAAA GAAGAGGGTA TTGAGTTCAA AATTGGAAAA 
TTCCCCTTTG CTGCAAACAG CAGAGCTAAA ACAAATGCTG ACACAGATGG CATGGTGAAG 
ATTCTTGGAC ATAAGTCAAC AGATAGAGTG CTGGGAGCAC ATATTCTAGG ACCAGGTGCT 
GGAGAAATGG TGAACGAGGC GGCCCTGGCA TTGGAATATG GTGCTTCCTG TGAAGATATA 
GCTCGAGTCT GCCATGCGCA TCCGACCTTA TCAGAGGCTT TTAGAGAAGC AAACTTGGCT 
GCAGCTTTTG GAAAACCAAT CAACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031887.2
CDS	133..1662
Translation	MQSWSRVYRSLAKKGHFNRISHGLQGVSSVPLRTYADQPIEADVTVIGSGPGGYVAAIKSAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEIPEVRLNLEKMMEQKHSAVKALTGGIAHLFKQNKVVHVNGFGKITGKNQVTATKADGSTQVIDTKNILVATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKLVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGIGIDMEISKNFQRILQRQGFKFKLNTKVTGATKKSDGKIDVSVEAASGGKAEVITCDVLLVCIGRRPFTQNLGLEELGIELDPKGRIPVNNRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEFKIGKFPFAANSRAKTNADTDGMVKILGHKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAAAFGKPINF

Target ORF information:

RefSeq Version	NM_007861.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus dihydrolipoamide dehydrogenase (Dld), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007861.5

ORF Insert Sequence:

ATGCAGAGCT GGAGTCGTGT GTACCGCTCC TTGGCCAAGA AAGGCCATTT CAATCGGATA 
TCTCATGGCC TACAAGGAGT TTCTTCAGTT CCTTTAAGAA CTTATGCAGA TCAACCAATT 
GAGGCTGACG TGACAGTGAT AGGTTCTGGC CCTGGAGGAT ATGTTGCTGC CATCAAATCT 
GCCCAGTTAG GCTTTAAGAC AGTCTGCATT GAGAAAAATG AGACACTAGG TGGAACATGC 
TTGAACGTTG GTTGTATTCC TTCAAAGGCT TTATTAAATA ATTCTCATTA TTACCATATG 
GCCCACGGAA AAGATTTTGC ATCTAGGGGA ATTGAAATAC CAGAAGTTCG CTTGAATTTA 
GAGAAGATGA TGGAGCAGAA GCATTCTGCA GTAAAAGCAT TAACAGGGGG AATTGCTCAC 
TTATTCAAAC AAAATAAGGT TGTTCACGTC AATGGATTTG GAAAGATAAC CGGCAAGAAT 
CAAGTTACAG CTACAAAAGC CGATGGCAGC ACTCAGGTTA TTGATACCAA AAACATCCTT 
GTAGCTACGG GCTCAGAAGT TACTCCTTTT CCTGGAATCA CGATCGATGA AGATACTATA 
GTGTCATCTA CAGGAGCTTT ATCCTTAAAA AAAGTTCCAG AGAAGCTGGT TGTTATTGGG 
GCAGGAGTAA TTGGTGTGGA ACTGGGTTCA GTTTGGCAAA GACTTGGTGC AGATGTGACA 
GCAGTTGAAT TTTTGGGTCA TGTGGGTGGA ATTGGAATTG ACATGGAGAT ATCAAAAAAT 
TTTCAACGTA TACTTCAGAG GCAAGGATTT AAGTTTAAAC TGAATACAAA AGTTACTGGT 
GCCACCAAGA AGTCAGATGG AAAAATTGAT GTGTCTGTCG AAGCTGCTTC TGGTGGTAAA 
GCTGAAGTTA TCACTTGTGA TGTACTCTTG GTTTGCATTG GTCGGCGGCC CTTTACTCAG 
AATTTGGGAC TAGAAGAGCT TGGAATTGAA CTAGATCCCA AAGGTAGAAT TCCAGTCAAT 
AACAGATTTC AAACTAAAAT TCCAAATATC TATGCTATTG GAGATGTGGT CGCTGGTCCA 
ATGTTGGCTC ACAAAGCAGA AGATGAAGGC ATCATCTGTG TTGAAGGGAT GGCCGGCGGT 
GCTGTGCACA TTGATTATAA TTGTGTGCCA TCAGTGATTT ACACACACCC CGAGGTTGCT 
TGGGTTGGCA AATCGGAAGA ACAGTTGAAA GAAGAGGGTA TTGAGTTCAA AATTGGAAAA 
TTCCCCTTTG CTGCAAACAG CAGAGCTAAA ACAAATGCTG ACACAGATGG CATGGTGAAG 
ATTCTTGGAC ATAAGTCAAC AGATAGAGTG CTGGGAGCAC ATATTCTAGG ACCAGGTGCT 
GGAGAAATGG TGAACGAGGC GGCCCTGGCA TTGGAATATG GTGCTTCCTG TGAAGATATA 
GCTCGAGTCT GCCATGCGCA TCCGACCTTA TCAGAGGCTT TTAGAGAAGC AAACTTGGCT 
GCAGCTTTTG GAAAACCAAT CAACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling.
Cell systems5(6)564-577.e12(2017 Dec)
Malty RH,Aoki H,Kumar A,Phanse S,Amin S,Zhang Q,Minic Z,Goebels F,Musso G,Wu Z,Abou-Tok H,Meyer M,Deineko V,Kassir S,Sidhu V,Jessulat M,Scott NE,Xiong X,Vlasblom J,Prasad B,Foster LJ,Alberio T,Garavaglia B,Yu H,Bader GD,Nakamura K,Parkinson J,Babu M

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Molecular and cellular biology36(15)2089-104(2016 Aug)
Bowman CE,Zhao L,Hartung T,Wolfgang MJ

Dihydrolipoyl dehydrogenase as a potential UVB target in skin epidermis; using an integrated approach of label-free quantitative proteomics and targeted metabolite analysis.
Journal of proteomics11770-85(2015 Mar)
Moon E,Park HM,Lee CH,Do SG,Park JM,Han NY,Do MH,Lee JH,Lee H,Kim SY

Mitochondrial Dihydrolipoamide Dehydrogenase is Upregulated in Response to Intermittent Hypoxic Preconditioning.
International journal of medical sciences12(5)432-40(2015)
Li R,Luo X,Wu J,Thangthaeng N,Jung ME,Jing S,Li L,Ellis DZ,Liu L,Ding Z,Forster MJ,Yan LJ

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Dld cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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