Diaph1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Diaph1
Entrez Gene ID	13367
Full Name	diaphanous related formin 1
Synonyms	D18Wsu154e,Dia1,Diap1,Drf1,p140mDia
General protein information	Preferred Names diaphanous related formin 1 Names protein diaphanous homolog 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 B3\|18 19.71 cM
Summary	This gene encodes a member of the formin family of proteins that play important roles in cytoskeletal rearragnement by nucleation of actin filaments. Mice lacking the encoded protein develop age-dependent myeloproliferative defects resembling human myeloproliferative syndrome and myelodysplastic syndromes. Trafficking of T lymphocytes to secondary lymphoid organs and egression of thymocytes from the thymus are impaired in these animals. Lack of the encoded protein in T lymphocytes and thymocytes also reduces chemotaxis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2016].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011246826.2	XP_011245128.1	protein diaphanous homolog 1 isoform X1
NM_007858.4	NP_031884.1	protein diaphanous homolog 1 isoform 2
NM_001305981.2	NP_001292910.1	protein diaphanous homolog 1 isoform 3
XM_030250316.1	XP_030106176.1	protein diaphanous homolog 1 isoform X1
NM_001305980.2	NP_001292909.1	protein diaphanous homolog 1 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIAPH1	NP_001073280.1
	DIAPH1	XP_001089232.2
Rattus norvegicus (Norway rat)	Diaph1	NP_001100863.2
Mus musculus (house mouse)	Diap1	NP_031884.1
Pan troglodytes (chimpanzee)	DIAPH1	XP_003310971.1
Drosophila melanogaster (fruit fly)	dia	NP_001246113.1
Canis lupus familiaris (dog)	DIAPH1	XP_005617363.1
Xenopus tropicalis (tropical clawed frog)	diaph1	XP_002940036.2

Gene Ontology

The following Diaph1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Diaph1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu87347	XM_030250316.1 Latest version!	Mus musculus diaphanous related formin 1 (Diaph1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu17522	NM_007858.4 Latest version!	Mus musculus diaphanous related formin 1 (Diaph1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$741.30 $1059.00
OMu67530	NM_001305980.2 Latest version!	Mus musculus diaphanous related formin 1 (Diaph1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu67532	NM_001305981.2 Latest version!	Mus musculus diaphanous related formin 1 (Diaph1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu67531	XM_011246826.2 Latest version!	Mus musculus diaphanous related formin 1 (Diaph1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Diaph1 ( XM_030250316.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106176.1 Mus musculus diaphanous related formin 1 (Diaph1), transcript variant X1, mRNA.

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CloneID	OMu87347
Clone ID Related Accession (Same CDS sequence)	XM_030250316.1
Accession Version	XM_030250316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGT CCGGCGGGGG CCTGGGGCCC GGCCGCGGTA CCCGGGACAA GAAGAAGGGT 
CGGAGCCCGG ATGAGCTGCC TGCGACGGGC GGCGACGGCG GCAAACATAA GAAATTTACT 
TTGAAGCGGC TCATGGCAGA CGAGCTGGAG AGATTTACCA GCATGAGGAT TAAGAAGGAG 
AAAGAAAAGC CCAATTCTGC TCATAGAAAC TCCTCTGCAT CGTACGGAGA TGACCCCACT 
GCTCAGTCAT TGCAGGACAT CTCAGACGAG CAAGTTCTTG TCCTCTTTGA GCAGATGCTG 
GTGGATATGA ACCTGAATGA GGAGAAGCAG CAGCCTTTGC GAGAGAAGGA CATTGTCATC 
AAGAGGGAGA TGGTGTCGCA ATATCTGCAC ACTTCCAAGG CTGGCATGAA CCAGAAAGAG 
AGCTCTAGGT CTGCCATGAT GTACATCCAG GAGCTGAGGT CGGGCTTGCG GGATATGCAC 
CTGCTTAGCT GCCTTGAGTC CCTTCGAGTC TCTCTCAACA ATAACCCTGT CAGTTGGGTG 
CAGACATTTG GTGCTGAGGG CCTAGCCTCC TTATTGGACA TCCTCAAACG ACTCCATGAT 
GAGAAAGAGG AGACTTCTGG AAACTACGAC AGCCGAAACC AGCATGAGAT TATCCGCTGT 
TTGAAGGCTT TCATGAACAA CAAGTTTGGA ATCAAAACTA TGTTGGAGAC GGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ATATGATGAT TGATGCAGCA 
AAGCTGCTGT CTGCCCTCTG TATCCTGCCG CAGCCGGAGG ACATGAATGA ACGAGTTCTA 
GAGGCAATGA CAGAGAGAGC TGAAATGGAT GAGGTCGAAC GCTTCCAGCC ACTTCTGGAC 
GGATTAAAAA GTGGGACCTC TATTGCCCTC AAAGTGGGAT GCCTACAGCT CATCAATGCT 
CTCATCACTC CAGCTGAGGA ACTGGACTTC CGAGTTCACA TCCGAAGTGA GCTGATGCGC 
CTGGGGCTGC ATCAGGTGTT GCAGGAGCTT CGAGAGATTG AAAATGAAGA TATGAAAGTA 
CAGCTGTGCG TGTTTGATGA ACAAGGGGAT GAAGATTTCT TTGATCTGAA GGGACGGCTG 
GATGATATCC GCATGGAGAT GGATGACTTT GGTGAAGTTT TTCAGATTAT TTTAAACACA 
GTGAAAGATT CAAAGGCAGA GCCACACTTC CTGTCTATCT TGCAGCATCT CCTGTTGGTC 
CGAAATGATT ATGAAGCCAG GCCACAGTAC TATAAACTGA TTGAAGAATG TGTTTCTCAG 
ATAGTTCTAC ACAAAAATGG AACTGATCCT GACTTCAAGT GCCGACACCT GCAGATTGAT 
ATTGAGAGAT TGGTTGATCA AATGATTGAT AAAACAAAGG TGGAAAAATC TGAGGCCAAA 
GCTACAGAGC TGGAAAAAAA GTTGGATTCA GAATTAACAG CGCGGCACGA GTTACAAGTA 
GAAATGAAAA AGATGGAAAA TGACTTTGAG CAGAAACTTC AGGATCTTCA AGGAGAAAAG 
GATGCCTTGG ATTCTGAAAA GCAGCAGATC ACTGCACAGA AACAAGACCT GGAGGCAGAG 
GTGTCCAAGC TGACAGGAGA GGTTGCCAAG CTGTCAAAAG AACTAGAAGA TGCCAAGAAT 
GAAATGGCTT CTCTCTCTGC TGTGGTTGTT GCACCTTCTG TTTCTAGCAG TGCTGCTGTT 
CCCCCTGCCC CTCCTCTGCC TGGTGACTCT GGCACTGTTA TTCCACCTCC CCCACCCCCA 
CCTCCTCTTC CTGGAGGTGT GGTCCCACCA TCCCCTCCTC TGCCTCCAGG TACTTGTATC 
CCTCCACCTC CTCCTTTACC TGGAGGTGCT TGTATACCCC CTCCCCCCCA GTTGCCTGGC 
AGTGCTGCCA TCCCTCCACC TCCTCCTCTA CCTGGAGTTG CTTCCATCCC CCCACCTCCC 
CCTTTGCCTG GGGCTACTGC CATCCCCCCA CCTCCCCCTT TGCCTGGGGC TACTGCCATC 
CCCCCACCTC CCCCTTTGCC TGGAGGTACA GGTATACCAC CACCACCTCC TCCTTTGCCT 
GGAAGTGTTG GCGTTCCCCC ACCCCCTCCC TTGCCTGGAG GACCAGGACT GCCTCCTCCC 
CCTCCCCCTT TTCCTGGAGC ACCTGGCATT CCTCCACCTC CACCTGGTAT GGGCGTGCCT 
CCACCTCCCC CCTTTGGATT TGGGGTTCCT GCGGCCCCAG TTCTGCCATT TGGATTAACC 
CCCAAAAAAG TTTATAAGCC AGAGGTGCAG CTCCGGAGGC CAAACTGGTC CAAGTTTGTG 
GCTGAGGACC TTTCCCAGGA CTGCTTCTGG ACAAAGGTGA AGGAGGACCG CTTTGAGAAC 
AATGAACTTT TTGCCAAACT TACCCTTGCC TTCTCCGCCC AGACCAAGAC TTCTAAAGTC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106176.1
CDS	305..2770
Translation	MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVVVAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLPGSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKVF

Target ORF information:

RefSeq Version	XM_030250316.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 1 (Diaph1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250316.1

ORF Insert Sequence:

ATGGAGCCGT CCGGCGGGGG CCTGGGGCCC GGCCGCGGTA CCCGGGACAA GAAGAAGGGT 
CGGAGCCCGG ATGAGCTGCC TGCGACGGGC GGCGACGGCG GCAAACATAA GAAATTTACT 
TTGAAGCGGC TCATGGCAGA CGAGCTGGAG AGATTTACCA GCATGAGGAT TAAGAAGGAG 
AAAGAAAAGC CCAATTCTGC TCATAGAAAC TCCTCTGCAT CGTACGGAGA TGACCCCACT 
GCTCAGTCAT TGCAGGACAT CTCAGACGAG CAAGTTCTTG TCCTCTTTGA GCAGATGCTG 
GTGGATATGA ACCTGAATGA GGAGAAGCAG CAGCCTTTGC GAGAGAAGGA CATTGTCATC 
AAGAGGGAGA TGGTGTCGCA ATATCTGCAC ACTTCCAAGG CTGGCATGAA CCAGAAAGAG 
AGCTCTAGGT CTGCCATGAT GTACATCCAG GAGCTGAGGT CGGGCTTGCG GGATATGCAC 
CTGCTTAGCT GCCTTGAGTC CCTTCGAGTC TCTCTCAACA ATAACCCTGT CAGTTGGGTG 
CAGACATTTG GTGCTGAGGG CCTAGCCTCC TTATTGGACA TCCTCAAACG ACTCCATGAT 
GAGAAAGAGG AGACTTCTGG AAACTACGAC AGCCGAAACC AGCATGAGAT TATCCGCTGT 
TTGAAGGCTT TCATGAACAA CAAGTTTGGA ATCAAAACTA TGTTGGAGAC GGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ATATGATGAT TGATGCAGCA 
AAGCTGCTGT CTGCCCTCTG TATCCTGCCG CAGCCGGAGG ACATGAATGA ACGAGTTCTA 
GAGGCAATGA CAGAGAGAGC TGAAATGGAT GAGGTCGAAC GCTTCCAGCC ACTTCTGGAC 
GGATTAAAAA GTGGGACCTC TATTGCCCTC AAAGTGGGAT GCCTACAGCT CATCAATGCT 
CTCATCACTC CAGCTGAGGA ACTGGACTTC CGAGTTCACA TCCGAAGTGA GCTGATGCGC 
CTGGGGCTGC ATCAGGTGTT GCAGGAGCTT CGAGAGATTG AAAATGAAGA TATGAAAGTA 
CAGCTGTGCG TGTTTGATGA ACAAGGGGAT GAAGATTTCT TTGATCTGAA GGGACGGCTG 
GATGATATCC GCATGGAGAT GGATGACTTT GGTGAAGTTT TTCAGATTAT TTTAAACACA 
GTGAAAGATT CAAAGGCAGA GCCACACTTC CTGTCTATCT TGCAGCATCT CCTGTTGGTC 
CGAAATGATT ATGAAGCCAG GCCACAGTAC TATAAACTGA TTGAAGAATG TGTTTCTCAG 
ATAGTTCTAC ACAAAAATGG AACTGATCCT GACTTCAAGT GCCGACACCT GCAGATTGAT 
ATTGAGAGAT TGGTTGATCA AATGATTGAT AAAACAAAGG TGGAAAAATC TGAGGCCAAA 
GCTACAGAGC TGGAAAAAAA GTTGGATTCA GAATTAACAG CGCGGCACGA GTTACAAGTA 
GAAATGAAAA AGATGGAAAA TGACTTTGAG CAGAAACTTC AGGATCTTCA AGGAGAAAAG 
GATGCCTTGG ATTCTGAAAA GCAGCAGATC ACTGCACAGA AACAAGACCT GGAGGCAGAG 
GTGTCCAAGC TGACAGGAGA GGTTGCCAAG CTGTCAAAAG AACTAGAAGA TGCCAAGAAT 
GAAATGGCTT CTCTCTCTGC TGTGGTTGTT GCACCTTCTG TTTCTAGCAG TGCTGCTGTT 
CCCCCTGCCC CTCCTCTGCC TGGTGACTCT GGCACTGTTA TTCCACCTCC CCCACCCCCA 
CCTCCTCTTC CTGGAGGTGT GGTCCCACCA TCCCCTCCTC TGCCTCCAGG TACTTGTATC 
CCTCCACCTC CTCCTTTACC TGGAGGTGCT TGTATACCCC CTCCCCCCCA GTTGCCTGGC 
AGTGCTGCCA TCCCTCCACC TCCTCCTCTA CCTGGAGTTG CTTCCATCCC CCCACCTCCC 
CCTTTGCCTG GGGCTACTGC CATCCCCCCA CCTCCCCCTT TGCCTGGGGC TACTGCCATC 
CCCCCACCTC CCCCTTTGCC TGGAGGTACA GGTATACCAC CACCACCTCC TCCTTTGCCT 
GGAAGTGTTG GCGTTCCCCC ACCCCCTCCC TTGCCTGGAG GACCAGGACT GCCTCCTCCC 
CCTCCCCCTT TTCCTGGAGC ACCTGGCATT CCTCCACCTC CACCTGGTAT GGGCGTGCCT 
CCACCTCCCC CCTTTGGATT TGGGGTTCCT GCGGCCCCAG TTCTGCCATT TGGATTAACC 
CCCAAAAAAG TTTATAAGCC AGAGGTGCAG CTCCGGAGGC CAAACTGGTC CAAGTTTGTG 
GCTGAGGACC TTTCCCAGGA CTGCTTCTGG ACAAAGGTGA AGGAGGACCG CTTTGAGAAC 
AATGAACTTT TTGCCAAACT TACCCTTGCC TTCTCCGCCC AGACCAAGAC TTCTAAAGTC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph1 ( NM_007858.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031884.1 Mus musculus diaphanous related formin 1 (Diaph1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu17522
Clone ID Related Accession (Same CDS sequence)	NM_007858.4
Accession Version	NM_007858.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC129315.3. On Sep 8, 2016 this sequence version replaced NM_007858.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U96963.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGT CCGGCGGGGG CCTGGGGCCC GGCCGCGGTA CCCGGGACAA GAAGAAGGGT 
CGGAGCCCGG ATGAGCTGCC TGCGACGGGC GGCGACGGCG GCAAACATAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG GATTAAGAAG GAGAAAGAAA AGCCCAATTC TGCTCATAGA 
AACTCCTCTG CATCGTACGG AGATGACCCC ACTGCTCAGT CATTGCAGGA CATCTCAGAC 
GAGCAAGTTC TTGTCCTCTT TGAGCAGATG CTGGTGGATA TGAACCTGAA TGAGGAGAAG 
CAGCAGCCTT TGCGAGAGAA GGACATTGTC ATCAAGAGGG AGATGGTGTC GCAATATCTG 
CACACTTCCA AGGCTGGCAT GAACCAGAAA GAGAGCTCTA GGTCTGCCAT GATGTACATC 
CAGGAGCTGA GGTCGGGCTT GCGGGATATG CACCTGCTTA GCTGCCTTGA GTCCCTTCGA 
GTCTCTCTCA ACAATAACCC TGTCAGTTGG GTGCAGACAT TTGGTGCTGA GGGCCTAGCC 
TCCTTATTGG ACATCCTCAA ACGACTCCAT GATGAGAAAG AGGAGACTTC TGGAAACTAC 
GACAGCCGAA ACCAGCATGA GATTATCCGC TGTTTGAAGG CTTTCATGAA CAACAAGTTT 
GGAATCAAAA CTATGTTGGA GACGGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT 
CCTGCTGTTC CCAATATGAT GATTGATGCA GCAAAGCTGC TGTCTGCCCT CTGTATCCTG 
CCGCAGCCGG AGGACATGAA TGAACGAGTT CTAGAGGCAA TGACAGAGAG AGCTGAAATG 
GATGAGGTCG AACGCTTCCA GCCACTTCTG GACGGATTAA AAAGTGGGAC CTCTATTGCC 
CTCAAAGTGG GATGCCTACA GCTCATCAAT GCTCTCATCA CTCCAGCTGA GGAACTGGAC 
TTCCGAGTTC ACATCCGAAG TGAGCTGATG CGCCTGGGGC TGCATCAGGT GTTGCAGGAG 
CTTCGAGAGA TTGAAAATGA AGATATGAAA GTACAGCTGT GCGTGTTTGA TGAACAAGGG 
GATGAAGATT TCTTTGATCT GAAGGGACGG CTGGATGATA TCCGCATGGA GATGGATGAC 
TTTGGTGAAG TTTTTCAGAT TATTTTAAAC ACAGTGAAAG ATTCAAAGGC AGAGCCACAC 
TTCCTGTCTA TCTTGCAGCA TCTCCTGTTG GTCCGAAATG ATTATGAAGC CAGGCCACAG 
TACTATAAAC TGATTGAAGA ATGTGTTTCT CAGATAGTTC TACACAAAAA TGGAACTGAT 
CCTGACTTCA AGTGCCGACA CCTGCAGATT GATATTGAGA GATTGGTTGA TCAAATGATT 
GATAAAACAA AGGTGGAAAA ATCTGAGGCC AAAGCTACAG AGCTGGAAAA AAAGTTGGAT 
TCAGAATTAA CAGCGCGGCA CGAGTTACAA GTAGAAATGA AAAAGATGGA AAATGACTTT 
GAGCAGAAAC TTCAGGATCT TCAAGGAGAA AAGGATGCCT TGGATTCTGA AAAGCAGCAG 
ATCACTGCAC AGAAACAAGA CCTGGAGGCA GAGGTGTCCA AGCTGACAGG AGAGGTTGCC 
AAGCTGTCAA AAGAACTAGA AGATGCCAAG AATGAAATGG CTTCTCTCTC TGCTGTGGTT 
GTTGCACCTT CTGTTTCTAG CAGTGCTGCT GTTCCCCCTG CCCCTCCTCT GCCTGGTGAC 
TCTGGCACTG TTATTCCACC TCCCCCACCC CCACCTCCTC TTCCTGGAGG TGTGGTCCCA 
CCATCCCCTC CTCTGCCTCC AGGTACTTGT ATCCCTCCAC CTCCTCCTTT ACCTGGAGGT 
GCTTGTATAC CCCCTCCCCC CCAGTTGCCT GGCAGTGCTG CCATCCCTCC ACCTCCTCCT 
CTACCTGGAG TTGCTTCCAT CCCCCCACCT CCCCCTTTGC CTGGGGCTAC TGCCATCCCC 
CCACCTCCCC CTTTGCCTGG GGCTACTGCC ATCCCCCCAC CTCCCCCTTT GCCTGGAGGT 
ACAGGTATAC CACCACCACC TCCTCCTTTG CCTGGAAGTG TTGGCGTTCC CCCACCCCCT 
CCCTTGCCTG GAGGACCAGG ACTGCCTCCT CCCCCTCCCC CTTTTCCTGG AGCACCTGGC 
ATTCCTCCAC CTCCACCTGG TATGGGCGTG CCTCCACCTC CCCCCTTTGG ATTTGGGGTT 
CCTGCGGCCC CAGTTCTGCC ATTTGGATTA ACCCCCAAAA AAGTTTATAA GCCAGAGGTG 
CAGCTCCGGA GGCCAAACTG GTCCAAGTTT GTGGCTGAGG ACCTTTCCCA GGACTGCTTC 
TGGACAAAGG TGAAGGAGGA CCGCTTTGAG AACAATGAAC TTTTTGCCAA ACTTACCCTT 
GCCTTCTCCG CCCAGACCAA GACTTCTAAA GCCAAGAAGG ATCAAGAAGG TGGAGAAGAA 
AAGAAATCTG TTCAAAAGAA GAAAGTAAAA GAGCTGAAAG TGCTGGATTC AAAGACAGCG 
CAGAATCTCT CAATCTTTTT GGGTTCATTC CGCATGCCCT ATCAAGAGAT AAAGAACGTT 
ATCCTGGAGG TGAATGAGGC TGTTCTCACA GAGTCTATGA TCCAGAACCT CATTAAACAG 
ATGCCAGAGC CAGAGCAGCT AAAGATGCTC TCTGAACTGA AGGAGGAGTA CGATGATCTG 
GCTGAGTCAG AGCAGTTTGG TGTGGTGATG GGCACAGTGC CCCGCCTTCG GCCTCGCCTC 
AACGCCATCC TCTTCAAGCT ACAGTTCAGT GAGCAAGTTG AGAACATCAA GCCAGAGATC 
GTGTCTGTCA CCGCCGCATG CGAAGAGCTG CGTAAGAGTG AGAACTTCTC CAGCCTCCTG 
GAGCTCACAC TGCTGGTCGG AAACTATATG AATGCGGGCT CCAGGAATGC TGGTGCTTTC 
GGCTTCAATA TCAGCTTCCT TTGTAAGCTT CGAGACACCA AGTCTGCAGA TCAGAAGATG 
ACTCTGTTGC ATTTCTTGGC TGAGTTATGT GAGAATGACC ACCCCGAAGT CCTCAAGTTT 
CCTGATGAGC TTGCCCATGT AGAGAAAGCC AGCAGAGTCT CTGCTGAGAA CCTGCAGAAG 
AGCTTAGATC AGATGAAGAA GCAGATTGCG GACGTGGAGC GCGATGTTCA GAATTTCCCA 
GCTGCCACTG ACGAGAAGGA CAAGTTTGTT GAGAAGATGA CCAGCTTTGT GAAGGATGCA 
CAGGAACAGT ATAACAAACT ACGGATGATG CACTCCAACA TGGAGACCCT CTATAAGGAG 
CTAGGTGACT ACTTCGTCTT TGACCCTAAG AAGTTGTCTG TAGAGGAATT CTTTATGGAT 
CTGCACAACT TTAGGAATAT GTTTTTGCAA GCAGTCAAGG AAAACCAGAA GCGCCGGGAA 
ACAGAAGAAA AGATGCGGAG AGCAAAATTA GCCAAGGAGA AGGCAGAAAA AGAGCGACTG 
GAGAAGCAGC AGAAGCGCGA GCAGCTCATC GACATGAACG CAGAGGGGGA TGAGACAGGT 
GTGATGGACA GTCTTCTAGA AGCTCTGCAG TCAGGGGCAG CATTCCGACG GAAGAGAGGG 
CCCCGGCAGG TCAACAGGAA GGCTGGGTGT GCAGTCACAT CTCTGCTAGC CTCGGAGCTG 
ACCAAGGATG ATGCCATGGC TCCTGGTCCT GTTAAGGTAC CCAAGAAAAG TGAAGGAGTC 
CCCACAATCC TGGAAGAAGC CAAGGAGCTG GTTGGCCGTG CAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031884.1
CDS	159..3926
Translation	MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVVVAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLPGSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCENDHPEVLKFPDELAHVEKASRVSAENLQKSLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAMAPGPVKVPKKSEGVPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	NM_007858.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 1 (Diaph1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007858.4

ORF Insert Sequence:

ATGGAGCCGT CCGGCGGGGG CCTGGGGCCC GGCCGCGGTA CCCGGGACAA GAAGAAGGGT 
CGGAGCCCGG ATGAGCTGCC TGCGACGGGC GGCGACGGCG GCAAACATAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG GATTAAGAAG GAGAAAGAAA AGCCCAATTC TGCTCATAGA 
AACTCCTCTG CATCGTACGG AGATGACCCC ACTGCTCAGT CATTGCAGGA CATCTCAGAC 
GAGCAAGTTC TTGTCCTCTT TGAGCAGATG CTGGTGGATA TGAACCTGAA TGAGGAGAAG 
CAGCAGCCTT TGCGAGAGAA GGACATTGTC ATCAAGAGGG AGATGGTGTC GCAATATCTG 
CACACTTCCA AGGCTGGCAT GAACCAGAAA GAGAGCTCTA GGTCTGCCAT GATGTACATC 
CAGGAGCTGA GGTCGGGCTT GCGGGATATG CACCTGCTTA GCTGCCTTGA GTCCCTTCGA 
GTCTCTCTCA ACAATAACCC TGTCAGTTGG GTGCAGACAT TTGGTGCTGA GGGCCTAGCC 
TCCTTATTGG ACATCCTCAA ACGACTCCAT GATGAGAAAG AGGAGACTTC TGGAAACTAC 
GACAGCCGAA ACCAGCATGA GATTATCCGC TGTTTGAAGG CTTTCATGAA CAACAAGTTT 
GGAATCAAAA CTATGTTGGA GACGGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT 
CCTGCTGTTC CCAATATGAT GATTGATGCA GCAAAGCTGC TGTCTGCCCT CTGTATCCTG 
CCGCAGCCGG AGGACATGAA TGAACGAGTT CTAGAGGCAA TGACAGAGAG AGCTGAAATG 
GATGAGGTCG AACGCTTCCA GCCACTTCTG GACGGATTAA AAAGTGGGAC CTCTATTGCC 
CTCAAAGTGG GATGCCTACA GCTCATCAAT GCTCTCATCA CTCCAGCTGA GGAACTGGAC 
TTCCGAGTTC ACATCCGAAG TGAGCTGATG CGCCTGGGGC TGCATCAGGT GTTGCAGGAG 
CTTCGAGAGA TTGAAAATGA AGATATGAAA GTACAGCTGT GCGTGTTTGA TGAACAAGGG 
GATGAAGATT TCTTTGATCT GAAGGGACGG CTGGATGATA TCCGCATGGA GATGGATGAC 
TTTGGTGAAG TTTTTCAGAT TATTTTAAAC ACAGTGAAAG ATTCAAAGGC AGAGCCACAC 
TTCCTGTCTA TCTTGCAGCA TCTCCTGTTG GTCCGAAATG ATTATGAAGC CAGGCCACAG 
TACTATAAAC TGATTGAAGA ATGTGTTTCT CAGATAGTTC TACACAAAAA TGGAACTGAT 
CCTGACTTCA AGTGCCGACA CCTGCAGATT GATATTGAGA GATTGGTTGA TCAAATGATT 
GATAAAACAA AGGTGGAAAA ATCTGAGGCC AAAGCTACAG AGCTGGAAAA AAAGTTGGAT 
TCAGAATTAA CAGCGCGGCA CGAGTTACAA GTAGAAATGA AAAAGATGGA AAATGACTTT 
GAGCAGAAAC TTCAGGATCT TCAAGGAGAA AAGGATGCCT TGGATTCTGA AAAGCAGCAG 
ATCACTGCAC AGAAACAAGA CCTGGAGGCA GAGGTGTCCA AGCTGACAGG AGAGGTTGCC 
AAGCTGTCAA AAGAACTAGA AGATGCCAAG AATGAAATGG CTTCTCTCTC TGCTGTGGTT 
GTTGCACCTT CTGTTTCTAG CAGTGCTGCT GTTCCCCCTG CCCCTCCTCT GCCTGGTGAC 
TCTGGCACTG TTATTCCACC TCCCCCACCC CCACCTCCTC TTCCTGGAGG TGTGGTCCCA 
CCATCCCCTC CTCTGCCTCC AGGTACTTGT ATCCCTCCAC CTCCTCCTTT ACCTGGAGGT 
GCTTGTATAC CCCCTCCCCC CCAGTTGCCT GGCAGTGCTG CCATCCCTCC ACCTCCTCCT 
CTACCTGGAG TTGCTTCCAT CCCCCCACCT CCCCCTTTGC CTGGGGCTAC TGCCATCCCC 
CCACCTCCCC CTTTGCCTGG GGCTACTGCC ATCCCCCCAC CTCCCCCTTT GCCTGGAGGT 
ACAGGTATAC CACCACCACC TCCTCCTTTG CCTGGAAGTG TTGGCGTTCC CCCACCCCCT 
CCCTTGCCTG GAGGACCAGG ACTGCCTCCT CCCCCTCCCC CTTTTCCTGG AGCACCTGGC 
ATTCCTCCAC CTCCACCTGG TATGGGCGTG CCTCCACCTC CCCCCTTTGG ATTTGGGGTT 
CCTGCGGCCC CAGTTCTGCC ATTTGGATTA ACCCCCAAAA AAGTTTATAA GCCAGAGGTG 
CAGCTCCGGA GGCCAAACTG GTCCAAGTTT GTGGCTGAGG ACCTTTCCCA GGACTGCTTC 
TGGACAAAGG TGAAGGAGGA CCGCTTTGAG AACAATGAAC TTTTTGCCAA ACTTACCCTT 
GCCTTCTCCG CCCAGACCAA GACTTCTAAA GCCAAGAAGG ATCAAGAAGG TGGAGAAGAA 
AAGAAATCTG TTCAAAAGAA GAAAGTAAAA GAGCTGAAAG TGCTGGATTC AAAGACAGCG 
CAGAATCTCT CAATCTTTTT GGGTTCATTC CGCATGCCCT ATCAAGAGAT AAAGAACGTT 
ATCCTGGAGG TGAATGAGGC TGTTCTCACA GAGTCTATGA TCCAGAACCT CATTAAACAG 
ATGCCAGAGC CAGAGCAGCT AAAGATGCTC TCTGAACTGA AGGAGGAGTA CGATGATCTG 
GCTGAGTCAG AGCAGTTTGG TGTGGTGATG GGCACAGTGC CCCGCCTTCG GCCTCGCCTC 
AACGCCATCC TCTTCAAGCT ACAGTTCAGT GAGCAAGTTG AGAACATCAA GCCAGAGATC 
GTGTCTGTCA CCGCCGCATG CGAAGAGCTG CGTAAGAGTG AGAACTTCTC CAGCCTCCTG 
GAGCTCACAC TGCTGGTCGG AAACTATATG AATGCGGGCT CCAGGAATGC TGGTGCTTTC 
GGCTTCAATA TCAGCTTCCT TTGTAAGCTT CGAGACACCA AGTCTGCAGA TCAGAAGATG 
ACTCTGTTGC ATTTCTTGGC TGAGTTATGT GAGAATGACC ACCCCGAAGT CCTCAAGTTT 
CCTGATGAGC TTGCCCATGT AGAGAAAGCC AGCAGAGTCT CTGCTGAGAA CCTGCAGAAG 
AGCTTAGATC AGATGAAGAA GCAGATTGCG GACGTGGAGC GCGATGTTCA GAATTTCCCA 
GCTGCCACTG ACGAGAAGGA CAAGTTTGTT GAGAAGATGA CCAGCTTTGT GAAGGATGCA 
CAGGAACAGT ATAACAAACT ACGGATGATG CACTCCAACA TGGAGACCCT CTATAAGGAG 
CTAGGTGACT ACTTCGTCTT TGACCCTAAG AAGTTGTCTG TAGAGGAATT CTTTATGGAT 
CTGCACAACT TTAGGAATAT GTTTTTGCAA GCAGTCAAGG AAAACCAGAA GCGCCGGGAA 
ACAGAAGAAA AGATGCGGAG AGCAAAATTA GCCAAGGAGA AGGCAGAAAA AGAGCGACTG 
GAGAAGCAGC AGAAGCGCGA GCAGCTCATC GACATGAACG CAGAGGGGGA TGAGACAGGT 
GTGATGGACA GTCTTCTAGA AGCTCTGCAG TCAGGGGCAG CATTCCGACG GAAGAGAGGG 
CCCCGGCAGG TCAACAGGAA GGCTGGGTGT GCAGTCACAT CTCTGCTAGC CTCGGAGCTG 
ACCAAGGATG ATGCCATGGC TCCTGGTCCT GTTAAGGTAC CCAAGAAAAG TGAAGGAGTC 
CCCACAATCC TGGAAGAAGC CAAGGAGCTG GTTGGCCGTG CAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph1 ( NM_001305980.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292909.1 Mus musculus diaphanous related formin 1 (Diaph1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu67530
Clone ID Related Accession (Same CDS sequence)	NM_001305980.2
Accession Version	NM_001305980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC129315.3. On Sep 8, 2016 this sequence version replaced NM_001305980.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK220466.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGT CCGGCGGGGG CCTGGGGCCC GGCCGCGGTA CCCGGGACAA GAAGAAGGGT 
CGGAGCCCGG ATGAGCTGCC TGCGACGGGC GGCGACGGCG GCAAACATAA GAAATTTACT 
TTGAAGCGGC TCATGGCAGA CGAGCTGGAG AGATTTACCA GCATGAGGAT TAAGAAGGAG 
AAAGAAAAGC CCAATTCTGC TCATAGAAAC TCCTCTGCAT CGTACGGAGA TGACCCCACT 
GCTCAGTCAT TGCAGGACAT CTCAGACGAG CAAGTTCTTG TCCTCTTTGA GCAGATGCTG 
GTGGATATGA ACCTGAATGA GGAGAAGCAG CAGCCTTTGC GAGAGAAGGA CATTGTCATC 
AAGAGGGAGA TGGTGTCGCA ATATCTGCAC ACTTCCAAGG CTGGCATGAA CCAGAAAGAG 
AGCTCTAGGT CTGCCATGAT GTACATCCAG GAGCTGAGGT CGGGCTTGCG GGATATGCAC 
CTGCTTAGCT GCCTTGAGTC CCTTCGAGTC TCTCTCAACA ATAACCCTGT CAGTTGGGTG 
CAGACATTTG GTGCTGAGGG CCTAGCCTCC TTATTGGACA TCCTCAAACG ACTCCATGAT 
GAGAAAGAGG AGACTTCTGG AAACTACGAC AGCCGAAACC AGCATGAGAT TATCCGCTGT 
TTGAAGGCTT TCATGAACAA CAAGTTTGGA ATCAAAACTA TGTTGGAGAC GGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ATATGATGAT TGATGCAGCA 
AAGCTGCTGT CTGCCCTCTG TATCCTGCCG CAGCCGGAGG ACATGAATGA ACGAGTTCTA 
GAGGCAATGA CAGAGAGAGC TGAAATGGAT GAGGTCGAAC GCTTCCAGCC ACTTCTGGAC 
GGATTAAAAA GTGGGACCTC TATTGCCCTC AAAGTGGGAT GCCTACAGCT CATCAATGCT 
CTCATCACTC CAGCTGAGGA ACTGGACTTC CGAGTTCACA TCCGAAGTGA GCTGATGCGC 
CTGGGGCTGC ATCAGGTGTT GCAGGAGCTT CGAGAGATTG AAAATGAAGA TATGAAAGTA 
CAGCTGTGCG TGTTTGATGA ACAAGGGGAT GAAGATTTCT TTGATCTGAA GGGACGGCTG 
GATGATATCC GCATGGAGAT GGATGACTTT GGTGAAGTTT TTCAGATTAT TTTAAACACA 
GTGAAAGATT CAAAGGCAGA GCCACACTTC CTGTCTATCT TGCAGCATCT CCTGTTGGTC 
CGAAATGATT ATGAAGCCAG GCCACAGTAC TATAAACTGA TTGAAGAATG TGTTTCTCAG 
ATAGTTCTAC ACAAAAATGG AACTGATCCT GACTTCAAGT GCCGACACCT GCAGATTGAT 
ATTGAGAGAT TGGTTGATCA AATGATTGAT AAAACAAAGG TGGAAAAATC TGAGGCCAAA 
GCTACAGAGC TGGAAAAAAA GTTGGATTCA GAATTAACAG CGCGGCACGA GTTACAAGTA 
GAAATGAAAA AGATGGAAAA TGACTTTGAG CAGAAACTTC AGGATCTTCA AGGAGAAAAG 
GATGCCTTGG ATTCTGAAAA GCAGCAGATC ACTGCACAGA AACAAGACCT GGAGGCAGAG 
GTGTCCAAGC TGACAGGAGA GGTTGCCAAG CTGTCAAAAG AACTAGAAGA TGCCAAGAAT 
GAAATGGCTT CTCTCTCTGC TGTGGTTGTT GCACCTTCTG TTTCTAGCAG TGCTGCTGTT 
CCCCCTGCCC CTCCTCTGCC TGGTGACTCT GGCACTGTTA TTCCACCTCC CCCACCCCCA 
CCTCCTCTTC CTGGAGGTGT GGTCCCACCA TCCCCTCCTC TGCCTCCAGG TACTTGTATC 
CCTCCACCTC CTCCTTTACC TGGAGGTGCT TGTATACCCC CTCCCCCCCA GTTGCCTGGC 
AGTGCTGCCA TCCCTCCACC TCCTCCTCTA CCTGGAGTTG CTTCCATCCC CCCACCTCCC 
CCTTTGCCTG GGGCTACTGC CATCCCCCCA CCTCCCCCTT TGCCTGGGGC TACTGCCATC 
CCCCCACCTC CCCCTTTGCC TGGAGGTACA GGTATACCAC CACCACCTCC TCCTTTGCCT 
GGAAGTGTTG GCGTTCCCCC ACCCCCTCCC TTGCCTGGAG GACCAGGACT GCCTCCTCCC 
CCTCCCCCTT TTCCTGGAGC ACCTGGCATT CCTCCACCTC CACCTGGTAT GGGCGTGCCT 
CCACCTCCCC CCTTTGGATT TGGGGTTCCT GCGGCCCCAG TTCTGCCATT TGGATTAACC 
CCCAAAAAAG TTTATAAGCC AGAGGTGCAG CTCCGGAGGC CAAACTGGTC CAAGTTTGTG 
GCTGAGGACC TTTCCCAGGA CTGCTTCTGG ACAAAGGTGA AGGAGGACCG CTTTGAGAAC 
AATGAACTTT TTGCCAAACT TACCCTTGCC TTCTCCGCCC AGACCAAGAC TTCTAAAGCC 
AAGAAGGATC AAGAAGGTGG AGAAGAAAAG AAATCTGTTC AAAAGAAGAA AGTAAAAGAG 
CTGAAAGTGC TGGATTCAAA GACAGCGCAG AATCTCTCAA TCTTTTTGGG TTCATTCCGC 
ATGCCCTATC AAGAGATAAA GAACGTTATC CTGGAGGTGA ATGAGGCTGT TCTCACAGAG 
TCTATGATCC AGAACCTCAT TAAACAGATG CCAGAGCCAG AGCAGCTAAA GATGCTCTCT 
GAACTGAAGG AGGAGTACGA TGATCTGGCT GAGTCAGAGC AGTTTGGTGT GGTGATGGGC 
ACAGTGCCCC GCCTTCGGCC TCGCCTCAAC GCCATCCTCT TCAAGCTACA GTTCAGTGAG 
CAAGTTGAGA ACATCAAGCC AGAGATCGTG TCTGTCACCG CCGCATGCGA AGAGCTGCGT 
AAGAGTGAGA ACTTCTCCAG CCTCCTGGAG CTCACACTGC TGGTCGGAAA CTATATGAAT 
GCGGGCTCCA GGAATGCTGG TGCTTTCGGC TTCAATATCA GCTTCCTTTG TAAGCTTCGA 
GACACCAAGT CTGCAGATCA GAAGATGACT CTGTTGCATT TCTTGGCTGA GTTATGTGAG 
AATGACCACC CCGAAGTCCT CAAGTTTCCT GATGAGCTTG CCCATGTAGA GAAAGCCAGC 
AGAGTCTCTG CTGAGAACCT GCAGAAGAGC TTAGATCAGA TGAAGAAGCA GATTGCGGAC 
GTGGAGCGCG ATGTTCAGAA TTTCCCAGCT GCCACTGACG AGAAGGACAA GTTTGTTGAG 
AAGATGACCA GCTTTGTGAA GGATGCACAG GAACAGTATA ACAAACTACG GATGATGCAC 
TCCAACATGG AGACCCTCTA TAAGGAGCTA GGTGACTACT TCGTCTTTGA CCCTAAGAAG 
TTGTCTGTAG AGGAATTCTT TATGGATCTG CACAACTTTA GGAATATGTT TTTGCAAGCA 
GTCAAGGAAA ACCAGAAGCG CCGGGAAACA GAAGAAAAGA TGCGGAGAGC AAAATTAGCC 
AAGGAGAAGG CAGAAAAAGA GCGACTGGAG AAGCAGCAGA AGCGCGAGCA GCTCATCGAC 
ATGAACGCAG AGGGGGATGA GACAGGTGTG ATGGACAGTC TTCTAGAAGC TCTGCAGTCA 
GGGGCAGCAT TCCGACGGAA GAGAGGGCCC CGGCAGGTCA ACAGGAAGGC TGGGTGTGCA 
GTCACATCTC TGCTAGCCTC GGAGCTGACC AAGGATGATG CCATGGCTCC TGGTCCTGTT 
AAGGTACCCA AGAAAAGTGA AGGAGTCCCC ACAATCCTGG AAGAAGCCAA GGAGCTGGTT 
GGCCGTGCAA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292909.1
CDS	159..3953
Translation	MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVVVAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLPGSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCENDHPEVLKFPDELAHVEKASRVSAENLQKSLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAMAPGPVKVPKKSEGVPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	NM_001305980.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 1 (Diaph1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305980.2

ORF Insert Sequence:

ATGGAGCCGT CCGGCGGGGG CCTGGGGCCC GGCCGCGGTA CCCGGGACAA GAAGAAGGGT 
CGGAGCCCGG ATGAGCTGCC TGCGACGGGC GGCGACGGCG GCAAACATAA GAAATTTACT 
TTGAAGCGGC TCATGGCAGA CGAGCTGGAG AGATTTACCA GCATGAGGAT TAAGAAGGAG 
AAAGAAAAGC CCAATTCTGC TCATAGAAAC TCCTCTGCAT CGTACGGAGA TGACCCCACT 
GCTCAGTCAT TGCAGGACAT CTCAGACGAG CAAGTTCTTG TCCTCTTTGA GCAGATGCTG 
GTGGATATGA ACCTGAATGA GGAGAAGCAG CAGCCTTTGC GAGAGAAGGA CATTGTCATC 
AAGAGGGAGA TGGTGTCGCA ATATCTGCAC ACTTCCAAGG CTGGCATGAA CCAGAAAGAG 
AGCTCTAGGT CTGCCATGAT GTACATCCAG GAGCTGAGGT CGGGCTTGCG GGATATGCAC 
CTGCTTAGCT GCCTTGAGTC CCTTCGAGTC TCTCTCAACA ATAACCCTGT CAGTTGGGTG 
CAGACATTTG GTGCTGAGGG CCTAGCCTCC TTATTGGACA TCCTCAAACG ACTCCATGAT 
GAGAAAGAGG AGACTTCTGG AAACTACGAC AGCCGAAACC AGCATGAGAT TATCCGCTGT 
TTGAAGGCTT TCATGAACAA CAAGTTTGGA ATCAAAACTA TGTTGGAGAC GGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ATATGATGAT TGATGCAGCA 
AAGCTGCTGT CTGCCCTCTG TATCCTGCCG CAGCCGGAGG ACATGAATGA ACGAGTTCTA 
GAGGCAATGA CAGAGAGAGC TGAAATGGAT GAGGTCGAAC GCTTCCAGCC ACTTCTGGAC 
GGATTAAAAA GTGGGACCTC TATTGCCCTC AAAGTGGGAT GCCTACAGCT CATCAATGCT 
CTCATCACTC CAGCTGAGGA ACTGGACTTC CGAGTTCACA TCCGAAGTGA GCTGATGCGC 
CTGGGGCTGC ATCAGGTGTT GCAGGAGCTT CGAGAGATTG AAAATGAAGA TATGAAAGTA 
CAGCTGTGCG TGTTTGATGA ACAAGGGGAT GAAGATTTCT TTGATCTGAA GGGACGGCTG 
GATGATATCC GCATGGAGAT GGATGACTTT GGTGAAGTTT TTCAGATTAT TTTAAACACA 
GTGAAAGATT CAAAGGCAGA GCCACACTTC CTGTCTATCT TGCAGCATCT CCTGTTGGTC 
CGAAATGATT ATGAAGCCAG GCCACAGTAC TATAAACTGA TTGAAGAATG TGTTTCTCAG 
ATAGTTCTAC ACAAAAATGG AACTGATCCT GACTTCAAGT GCCGACACCT GCAGATTGAT 
ATTGAGAGAT TGGTTGATCA AATGATTGAT AAAACAAAGG TGGAAAAATC TGAGGCCAAA 
GCTACAGAGC TGGAAAAAAA GTTGGATTCA GAATTAACAG CGCGGCACGA GTTACAAGTA 
GAAATGAAAA AGATGGAAAA TGACTTTGAG CAGAAACTTC AGGATCTTCA AGGAGAAAAG 
GATGCCTTGG ATTCTGAAAA GCAGCAGATC ACTGCACAGA AACAAGACCT GGAGGCAGAG 
GTGTCCAAGC TGACAGGAGA GGTTGCCAAG CTGTCAAAAG AACTAGAAGA TGCCAAGAAT 
GAAATGGCTT CTCTCTCTGC TGTGGTTGTT GCACCTTCTG TTTCTAGCAG TGCTGCTGTT 
CCCCCTGCCC CTCCTCTGCC TGGTGACTCT GGCACTGTTA TTCCACCTCC CCCACCCCCA 
CCTCCTCTTC CTGGAGGTGT GGTCCCACCA TCCCCTCCTC TGCCTCCAGG TACTTGTATC 
CCTCCACCTC CTCCTTTACC TGGAGGTGCT TGTATACCCC CTCCCCCCCA GTTGCCTGGC 
AGTGCTGCCA TCCCTCCACC TCCTCCTCTA CCTGGAGTTG CTTCCATCCC CCCACCTCCC 
CCTTTGCCTG GGGCTACTGC CATCCCCCCA CCTCCCCCTT TGCCTGGGGC TACTGCCATC 
CCCCCACCTC CCCCTTTGCC TGGAGGTACA GGTATACCAC CACCACCTCC TCCTTTGCCT 
GGAAGTGTTG GCGTTCCCCC ACCCCCTCCC TTGCCTGGAG GACCAGGACT GCCTCCTCCC 
CCTCCCCCTT TTCCTGGAGC ACCTGGCATT CCTCCACCTC CACCTGGTAT GGGCGTGCCT 
CCACCTCCCC CCTTTGGATT TGGGGTTCCT GCGGCCCCAG TTCTGCCATT TGGATTAACC 
CCCAAAAAAG TTTATAAGCC AGAGGTGCAG CTCCGGAGGC CAAACTGGTC CAAGTTTGTG 
GCTGAGGACC TTTCCCAGGA CTGCTTCTGG ACAAAGGTGA AGGAGGACCG CTTTGAGAAC 
AATGAACTTT TTGCCAAACT TACCCTTGCC TTCTCCGCCC AGACCAAGAC TTCTAAAGCC 
AAGAAGGATC AAGAAGGTGG AGAAGAAAAG AAATCTGTTC AAAAGAAGAA AGTAAAAGAG 
CTGAAAGTGC TGGATTCAAA GACAGCGCAG AATCTCTCAA TCTTTTTGGG TTCATTCCGC 
ATGCCCTATC AAGAGATAAA GAACGTTATC CTGGAGGTGA ATGAGGCTGT TCTCACAGAG 
TCTATGATCC AGAACCTCAT TAAACAGATG CCAGAGCCAG AGCAGCTAAA GATGCTCTCT 
GAACTGAAGG AGGAGTACGA TGATCTGGCT GAGTCAGAGC AGTTTGGTGT GGTGATGGGC 
ACAGTGCCCC GCCTTCGGCC TCGCCTCAAC GCCATCCTCT TCAAGCTACA GTTCAGTGAG 
CAAGTTGAGA ACATCAAGCC AGAGATCGTG TCTGTCACCG CCGCATGCGA AGAGCTGCGT 
AAGAGTGAGA ACTTCTCCAG CCTCCTGGAG CTCACACTGC TGGTCGGAAA CTATATGAAT 
GCGGGCTCCA GGAATGCTGG TGCTTTCGGC TTCAATATCA GCTTCCTTTG TAAGCTTCGA 
GACACCAAGT CTGCAGATCA GAAGATGACT CTGTTGCATT TCTTGGCTGA GTTATGTGAG 
AATGACCACC CCGAAGTCCT CAAGTTTCCT GATGAGCTTG CCCATGTAGA GAAAGCCAGC 
AGAGTCTCTG CTGAGAACCT GCAGAAGAGC TTAGATCAGA TGAAGAAGCA GATTGCGGAC 
GTGGAGCGCG ATGTTCAGAA TTTCCCAGCT GCCACTGACG AGAAGGACAA GTTTGTTGAG 
AAGATGACCA GCTTTGTGAA GGATGCACAG GAACAGTATA ACAAACTACG GATGATGCAC 
TCCAACATGG AGACCCTCTA TAAGGAGCTA GGTGACTACT TCGTCTTTGA CCCTAAGAAG 
TTGTCTGTAG AGGAATTCTT TATGGATCTG CACAACTTTA GGAATATGTT TTTGCAAGCA 
GTCAAGGAAA ACCAGAAGCG CCGGGAAACA GAAGAAAAGA TGCGGAGAGC AAAATTAGCC 
AAGGAGAAGG CAGAAAAAGA GCGACTGGAG AAGCAGCAGA AGCGCGAGCA GCTCATCGAC 
ATGAACGCAG AGGGGGATGA GACAGGTGTG ATGGACAGTC TTCTAGAAGC TCTGCAGTCA 
GGGGCAGCAT TCCGACGGAA GAGAGGGCCC CGGCAGGTCA ACAGGAAGGC TGGGTGTGCA 
GTCACATCTC TGCTAGCCTC GGAGCTGACC AAGGATGATG CCATGGCTCC TGGTCCTGTT 
AAGGTACCCA AGAAAAGTGA AGGAGTCCCC ACAATCCTGG AAGAAGCCAA GGAGCTGGTT 
GGCCGTGCAA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph1 ( NM_001305981.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292910.1 Mus musculus diaphanous related formin 1 (Diaph1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu67532
Clone ID Related Accession (Same CDS sequence)	NM_001305981.2
Accession Version	NM_001305981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC129315.3. On Sep 14, 2016 this sequence version replaced NM_001305981.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACG AGCTGGAGAG ATTTACCAGC ATGAGGATTA AGAAGGAGAA AGAAAAGCCC 
AATTCTGCTC ATAGAAACTC CTCTGCATCG TACGGAGATG ACCCCACTGC TCAGTCATTG 
CAGGACATCT CAGACGAGCA AGTTCTTGTC CTCTTTGAGC AGATGCTGGT GGATATGAAC 
CTGAATGAGG AGAAGCAGCA GCCTTTGCGA GAGAAGGACA TTGTCATCAA GAGGGAGATG 
GTGTCGCAAT ATCTGCACAC TTCCAAGGCT GGCATGAACC AGAAAGAGAG CTCTAGGTCT 
GCCATGATGT ACATCCAGGA GCTGAGGTCG GGCTTGCGGG ATATGCACCT GCTTAGCTGC 
CTTGAGTCCC TTCGAGTCTC TCTCAACAAT AACCCTGTCA GTTGGGTGCA GACATTTGGT 
GCTGAGGGCC TAGCCTCCTT ATTGGACATC CTCAAACGAC TCCATGATGA GAAAGAGGAG 
ACTTCTGGAA ACTACGACAG CCGAAACCAG CATGAGATTA TCCGCTGTTT GAAGGCTTTC 
ATGAACAACA AGTTTGGAAT CAAAACTATG TTGGAGACGG AAGAAGGAAT CCTACTGCTG 
GTCAGAGCCA TGGATCCTGC TGTTCCCAAT ATGATGATTG ATGCAGCAAA GCTGCTGTCT 
GCCCTCTGTA TCCTGCCGCA GCCGGAGGAC ATGAATGAAC GAGTTCTAGA GGCAATGACA 
GAGAGAGCTG AAATGGATGA GGTCGAACGC TTCCAGCCAC TTCTGGACGG ATTAAAAAGT 
GGGACCTCTA TTGCCCTCAA AGTGGGATGC CTACAGCTCA TCAATGCTCT CATCACTCCA 
GCTGAGGAAC TGGACTTCCG AGTTCACATC CGAAGTGAGC TGATGCGCCT GGGGCTGCAT 
CAGGTGTTGC AGGAGCTTCG AGAGATTGAA AATGAAGATA TGAAAGTACA GCTGTGCGTG 
TTTGATGAAC AAGGGGATGA AGATTTCTTT GATCTGAAGG GACGGCTGGA TGATATCCGC 
ATGGAGATGG ATGACTTTGG TGAAGTTTTT CAGATTATTT TAAACACAGT GAAAGATTCA 
AAGGCAGAGC CACACTTCCT GTCTATCTTG CAGCATCTCC TGTTGGTCCG AAATGATTAT 
GAAGCCAGGC CACAGTACTA TAAACTGATT GAAGAATGTG TTTCTCAGAT AGTTCTACAC 
AAAAATGGAA CTGATCCTGA CTTCAAGTGC CGACACCTGC AGATTGATAT TGAGAGATTG 
GTTGATCAAA TGATTGATAA AACAAAGGTG GAAAAATCTG AGGCCAAAGC TACAGAGCTG 
GAAAAAAAGT TGGATTCAGA ATTAACAGCG CGGCACGAGT TACAAGTAGA AATGAAAAAG 
ATGGAAAATG ACTTTGAGCA GAAACTTCAG GATCTTCAAG GAGAAAAGGA TGCCTTGGAT 
TCTGAAAAGC AGCAGATCAC TGCACAGAAA CAAGACCTGG AGGCAGAGGT GTCCAAGCTG 
ACAGGAGAGG TTGCCAAGCT GTCAAAAGAA CTAGAAGATG CCAAGAATGA AATGGCTTCT 
CTCTCTGCTG TGGTTGTTGC ACCTTCTGTT TCTAGCAGTG CTGCTGTTCC CCCTGCCCCT 
CCTCTGCCTG GTGACTCTGG CACTGTTATT CCACCTCCCC CACCCCCACC TCCTCTTCCT 
GGAGGTGTGG TCCCACCATC CCCTCCTCTG CCTCCAGGTA CTTGTATCCC TCCACCTCCT 
CCTTTACCTG GAGGTGCTTG TATACCCCCT CCCCCCCAGT TGCCTGGCAG TGCTGCCATC 
CCTCCACCTC CTCCTCTACC TGGAGTTGCT TCCATCCCCC CACCTCCCCC TTTGCCTGGG 
GCTACTGCCA TCCCCCCACC TCCCCCTTTG CCTGGGGCTA CTGCCATCCC CCCACCTCCC 
CCTTTGCCTG GAGGTACAGG TATACCACCA CCACCTCCTC CTTTGCCTGG AAGTGTTGGC 
GTTCCCCCAC CCCCTCCCTT GCCTGGAGGA CCAGGACTGC CTCCTCCCCC TCCCCCTTTT 
CCTGGAGCAC CTGGCATTCC TCCACCTCCA CCTGGTATGG GCGTGCCTCC ACCTCCCCCC 
TTTGGATTTG GGGTTCCTGC GGCCCCAGTT CTGCCATTTG GATTAACCCC CAAAAAAGTT 
TATAAGCCAG AGGTGCAGCT CCGGAGGCCA AACTGGTCCA AGTTTGTGGC TGAGGACCTT 
TCCCAGGACT GCTTCTGGAC AAAGGTGAAG GAGGACCGCT TTGAGAACAA TGAACTTTTT 
GCCAAACTTA CCCTTGCCTT CTCCGCCCAG ACCAAGACTT CTAAAGCCAA GAAGGATCAA 
GAAGGTGGAG AAGAAAAGAA ATCTGTTCAA AAGAAGAAAG TAAAAGAGCT GAAAGTGCTG 
GATTCAAAGA CAGCGCAGAA TCTCTCAATC TTTTTGGGTT CATTCCGCAT GCCCTATCAA 
GAGATAAAGA ACGTTATCCT GGAGGTGAAT GAGGCTGTTC TCACAGAGTC TATGATCCAG 
AACCTCATTA AACAGATGCC AGAGCCAGAG CAGCTAAAGA TGCTCTCTGA ACTGAAGGAG 
GAGTACGATG ATCTGGCTGA GTCAGAGCAG TTTGGTGTGG TGATGGGCAC AGTGCCCCGC 
CTTCGGCCTC GCCTCAACGC CATCCTCTTC AAGCTACAGT TCAGTGAGCA AGTTGAGAAC 
ATCAAGCCAG AGATCGTGTC TGTCACCGCC GCATGCGAAG AGCTGCGTAA GAGTGAGAAC 
TTCTCCAGCC TCCTGGAGCT CACACTGCTG GTCGGAAACT ATATGAATGC GGGCTCCAGG 
AATGCTGGTG CTTTCGGCTT CAATATCAGC TTCCTTTGTA AGCTTCGAGA CACCAAGTCT 
GCAGATCAGA AGATGACTCT GTTGCATTTC TTGGCTGAGT TATGTGAGAA TGACCACCCC 
GAAGTCCTCA AGTTTCCTGA TGAGCTTGCC CATGTAGAGA AAGCCAGCAG AGTCTCTGCT 
GAGAACCTGC AGAAGAGCTT AGATCAGATG AAGAAGCAGA TTGCGGACGT GGAGCGCGAT 
GTTCAGAATT TCCCAGCTGC CACTGACGAG AAGGACAAGT TTGTTGAGAA GATGACCAGC 
TTTGTGAAGG ATGCACAGGA ACAGTATAAC AAACTACGGA TGATGCACTC CAACATGGAG 
ACCCTCTATA AGGAGCTAGG TGACTACTTC GTCTTTGACC CTAAGAAGTT GTCTGTAGAG 
GAATTCTTTA TGGATCTGCA CAACTTTAGG AATATGTTTT TGCAAGCAGT CAAGGAAAAC 
CAGAAGCGCC GGGAAACAGA AGAAAAGATG CGGAGAGCAA AATTAGCCAA GGAGAAGGCA 
GAAAAAGAGC GACTGGAGAA GCAGCAGAAG CGCGAGCAGC TCATCGACAT GAACGCAGAG 
GGGGATGAGA CAGGTGTGAT GGACAGTCTT CTAGAAGCTC TGCAGTCAGG GGCAGCATTC 
CGACGGAAGA GAGGGCCCCG GCAGGTCAAC AGGAAGGCTG GGTGTGCAGT CACATCTCTG 
CTAGCCTCGG AGCTGACCAA GGATGATGCC ATGGCTCCTG GTCCTGTTAA GGTACCCAAG 
AAAAGTGAAG GAGTCCCCAC AATCCTGGAA GAAGCCAAGG AGCTGGTTGG CCGTGCAAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292910.1
CDS	394..4056
Translation	MADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVVVAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLPGSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCENDHPEVLKFPDELAHVEKASRVSAENLQKSLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAMAPGPVKVPKKSEGVPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	NM_001305981.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 1 (Diaph1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305981.2

ORF Insert Sequence:

ATGGCAGACG AGCTGGAGAG ATTTACCAGC ATGAGGATTA AGAAGGAGAA AGAAAAGCCC 
AATTCTGCTC ATAGAAACTC CTCTGCATCG TACGGAGATG ACCCCACTGC TCAGTCATTG 
CAGGACATCT CAGACGAGCA AGTTCTTGTC CTCTTTGAGC AGATGCTGGT GGATATGAAC 
CTGAATGAGG AGAAGCAGCA GCCTTTGCGA GAGAAGGACA TTGTCATCAA GAGGGAGATG 
GTGTCGCAAT ATCTGCACAC TTCCAAGGCT GGCATGAACC AGAAAGAGAG CTCTAGGTCT 
GCCATGATGT ACATCCAGGA GCTGAGGTCG GGCTTGCGGG ATATGCACCT GCTTAGCTGC 
CTTGAGTCCC TTCGAGTCTC TCTCAACAAT AACCCTGTCA GTTGGGTGCA GACATTTGGT 
GCTGAGGGCC TAGCCTCCTT ATTGGACATC CTCAAACGAC TCCATGATGA GAAAGAGGAG 
ACTTCTGGAA ACTACGACAG CCGAAACCAG CATGAGATTA TCCGCTGTTT GAAGGCTTTC 
ATGAACAACA AGTTTGGAAT CAAAACTATG TTGGAGACGG AAGAAGGAAT CCTACTGCTG 
GTCAGAGCCA TGGATCCTGC TGTTCCCAAT ATGATGATTG ATGCAGCAAA GCTGCTGTCT 
GCCCTCTGTA TCCTGCCGCA GCCGGAGGAC ATGAATGAAC GAGTTCTAGA GGCAATGACA 
GAGAGAGCTG AAATGGATGA GGTCGAACGC TTCCAGCCAC TTCTGGACGG ATTAAAAAGT 
GGGACCTCTA TTGCCCTCAA AGTGGGATGC CTACAGCTCA TCAATGCTCT CATCACTCCA 
GCTGAGGAAC TGGACTTCCG AGTTCACATC CGAAGTGAGC TGATGCGCCT GGGGCTGCAT 
CAGGTGTTGC AGGAGCTTCG AGAGATTGAA AATGAAGATA TGAAAGTACA GCTGTGCGTG 
TTTGATGAAC AAGGGGATGA AGATTTCTTT GATCTGAAGG GACGGCTGGA TGATATCCGC 
ATGGAGATGG ATGACTTTGG TGAAGTTTTT CAGATTATTT TAAACACAGT GAAAGATTCA 
AAGGCAGAGC CACACTTCCT GTCTATCTTG CAGCATCTCC TGTTGGTCCG AAATGATTAT 
GAAGCCAGGC CACAGTACTA TAAACTGATT GAAGAATGTG TTTCTCAGAT AGTTCTACAC 
AAAAATGGAA CTGATCCTGA CTTCAAGTGC CGACACCTGC AGATTGATAT TGAGAGATTG 
GTTGATCAAA TGATTGATAA AACAAAGGTG GAAAAATCTG AGGCCAAAGC TACAGAGCTG 
GAAAAAAAGT TGGATTCAGA ATTAACAGCG CGGCACGAGT TACAAGTAGA AATGAAAAAG 
ATGGAAAATG ACTTTGAGCA GAAACTTCAG GATCTTCAAG GAGAAAAGGA TGCCTTGGAT 
TCTGAAAAGC AGCAGATCAC TGCACAGAAA CAAGACCTGG AGGCAGAGGT GTCCAAGCTG 
ACAGGAGAGG TTGCCAAGCT GTCAAAAGAA CTAGAAGATG CCAAGAATGA AATGGCTTCT 
CTCTCTGCTG TGGTTGTTGC ACCTTCTGTT TCTAGCAGTG CTGCTGTTCC CCCTGCCCCT 
CCTCTGCCTG GTGACTCTGG CACTGTTATT CCACCTCCCC CACCCCCACC TCCTCTTCCT 
GGAGGTGTGG TCCCACCATC CCCTCCTCTG CCTCCAGGTA CTTGTATCCC TCCACCTCCT 
CCTTTACCTG GAGGTGCTTG TATACCCCCT CCCCCCCAGT TGCCTGGCAG TGCTGCCATC 
CCTCCACCTC CTCCTCTACC TGGAGTTGCT TCCATCCCCC CACCTCCCCC TTTGCCTGGG 
GCTACTGCCA TCCCCCCACC TCCCCCTTTG CCTGGGGCTA CTGCCATCCC CCCACCTCCC 
CCTTTGCCTG GAGGTACAGG TATACCACCA CCACCTCCTC CTTTGCCTGG AAGTGTTGGC 
GTTCCCCCAC CCCCTCCCTT GCCTGGAGGA CCAGGACTGC CTCCTCCCCC TCCCCCTTTT 
CCTGGAGCAC CTGGCATTCC TCCACCTCCA CCTGGTATGG GCGTGCCTCC ACCTCCCCCC 
TTTGGATTTG GGGTTCCTGC GGCCCCAGTT CTGCCATTTG GATTAACCCC CAAAAAAGTT 
TATAAGCCAG AGGTGCAGCT CCGGAGGCCA AACTGGTCCA AGTTTGTGGC TGAGGACCTT 
TCCCAGGACT GCTTCTGGAC AAAGGTGAAG GAGGACCGCT TTGAGAACAA TGAACTTTTT 
GCCAAACTTA CCCTTGCCTT CTCCGCCCAG ACCAAGACTT CTAAAGCCAA GAAGGATCAA 
GAAGGTGGAG AAGAAAAGAA ATCTGTTCAA AAGAAGAAAG TAAAAGAGCT GAAAGTGCTG 
GATTCAAAGA CAGCGCAGAA TCTCTCAATC TTTTTGGGTT CATTCCGCAT GCCCTATCAA 
GAGATAAAGA ACGTTATCCT GGAGGTGAAT GAGGCTGTTC TCACAGAGTC TATGATCCAG 
AACCTCATTA AACAGATGCC AGAGCCAGAG CAGCTAAAGA TGCTCTCTGA ACTGAAGGAG 
GAGTACGATG ATCTGGCTGA GTCAGAGCAG TTTGGTGTGG TGATGGGCAC AGTGCCCCGC 
CTTCGGCCTC GCCTCAACGC CATCCTCTTC AAGCTACAGT TCAGTGAGCA AGTTGAGAAC 
ATCAAGCCAG AGATCGTGTC TGTCACCGCC GCATGCGAAG AGCTGCGTAA GAGTGAGAAC 
TTCTCCAGCC TCCTGGAGCT CACACTGCTG GTCGGAAACT ATATGAATGC GGGCTCCAGG 
AATGCTGGTG CTTTCGGCTT CAATATCAGC TTCCTTTGTA AGCTTCGAGA CACCAAGTCT 
GCAGATCAGA AGATGACTCT GTTGCATTTC TTGGCTGAGT TATGTGAGAA TGACCACCCC 
GAAGTCCTCA AGTTTCCTGA TGAGCTTGCC CATGTAGAGA AAGCCAGCAG AGTCTCTGCT 
GAGAACCTGC AGAAGAGCTT AGATCAGATG AAGAAGCAGA TTGCGGACGT GGAGCGCGAT 
GTTCAGAATT TCCCAGCTGC CACTGACGAG AAGGACAAGT TTGTTGAGAA GATGACCAGC 
TTTGTGAAGG ATGCACAGGA ACAGTATAAC AAACTACGGA TGATGCACTC CAACATGGAG 
ACCCTCTATA AGGAGCTAGG TGACTACTTC GTCTTTGACC CTAAGAAGTT GTCTGTAGAG 
GAATTCTTTA TGGATCTGCA CAACTTTAGG AATATGTTTT TGCAAGCAGT CAAGGAAAAC 
CAGAAGCGCC GGGAAACAGA AGAAAAGATG CGGAGAGCAA AATTAGCCAA GGAGAAGGCA 
GAAAAAGAGC GACTGGAGAA GCAGCAGAAG CGCGAGCAGC TCATCGACAT GAACGCAGAG 
GGGGATGAGA CAGGTGTGAT GGACAGTCTT CTAGAAGCTC TGCAGTCAGG GGCAGCATTC 
CGACGGAAGA GAGGGCCCCG GCAGGTCAAC AGGAAGGCTG GGTGTGCAGT CACATCTCTG 
CTAGCCTCGG AGCTGACCAA GGATGATGCC ATGGCTCCTG GTCCTGTTAA GGTACCCAAG 
AAAAGTGAAG GAGTCCCCAC AATCCTGGAA GAAGCCAAGG AGCTGGTTGG CCGTGCAAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph1 ( XM_011246826.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245128.1 Mus musculus diaphanous related formin 1 (Diaph1), transcript variant X1, mRNA.

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CloneID	OMu67531
Clone ID Related Accession (Same CDS sequence)	XM_011246826.2
Accession Version	XM_011246826.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246826.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGT CCGGCGGGGG CCTGGGGCCC GGCCGCGGTA CCCGGGACAA GAAGAAGGGT 
CGGAGCCCGG ATGAGCTGCC TGCGACGGGC GGCGACGGCG GCAAACATAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG GATTAAGAAG GAGAAAGAAA AGCCCAATTC TGCTCATAGA 
AACTCCTCTG CATCGTACGG AGATGACCCC ACTGCTCAGT CATTGCAGGA CATCTCAGAC 
GAGCAAGTTC TTGTCCTCTT TGAGCAGATG CTGGTGGATA TGAACCTGAA TGAGGAGAAG 
CAGCAGCCTT TGCGAGAGAA GGACATTGTC ATCAAGAGGG AGATGGTGTC GCAATATCTG 
CACACTTCCA AGGCTGGCAT GAACCAGAAA GAGAGCTCTA GGTCTGCCAT GATGTACATC 
CAGGAGCTGA GGTCGGGCTT GCGGGATATG CACCTGCTTA GCTGCCTTGA GTCCCTTCGA 
GTCTCTCTCA ACAATAACCC TGTCAGTTGG GTGCAGACAT TTGGTGCTGA GGGCCTAGCC 
TCCTTATTGG ACATCCTCAA ACGACTCCAT GATGAGAAAG AGGAGACTTC TGGAAACTAC 
GACAGCCGAA ACCAGCATGA GATTATCCGC TGTTTGAAGG CTTTCATGAA CAACAAGTTT 
GGAATCAAAA CTATGTTGGA GACGGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT 
CCTGCTGTTC CCAATATGAT GATTGATGCA GCAAAGCTGC TGTCTGCCCT CTGTATCCTG 
CCGCAGCCGG AGGACATGAA TGAACGAGTT CTAGAGGCAA TGACAGAGAG AGCTGAAATG 
GATGAGGTCG AACGCTTCCA GCCACTTCTG GACGGATTAA AAAGTGGGAC CTCTATTGCC 
CTCAAAGTGG GATGCCTACA GCTCATCAAT GCTCTCATCA CTCCAGCTGA GGAACTGGAC 
TTCCGAGTTC ACATCCGAAG TGAGCTGATG CGCCTGGGGC TGCATCAGGT GTTGCAGGAG 
CTTCGAGAGA TTGAAAATGA AGATATGAAA GTACAGCTGT GCGTGTTTGA TGAACAAGGG 
GATGAAGATT TCTTTGATCT GAAGGGACGG CTGGATGATA TCCGCATGGA GATGGATGAC 
TTTGGTGAAG TTTTTCAGAT TATTTTAAAC ACAGTGAAAG ATTCAAAGGC AGAGCCACAC 
TTCCTGTCTA TCTTGCAGCA TCTCCTGTTG GTCCGAAATG ATTATGAAGC CAGGCCACAG 
TACTATAAAC TGATTGAAGA ATGTGTTTCT CAGATAGTTC TACACAAAAA TGGAACTGAT 
CCTGACTTCA AGTGCCGACA CCTGCAGATT GATATTGAGA GATTGGTTGA TCAAATGATT 
GATAAAACAA AGGTGGAAAA ATCTGAGGCC AAAGCTACAG AGCTGGAAAA AAAGTTGGAT 
TCAGAATTAA CAGCGCGGCA CGAGTTACAA GTAGAAATGA AAAAGATGGA AAATGACTTT 
GAGCAGAAAC TTCAGGATCT TCAAGGAGAA AAGGATGCCT TGGATTCTGA AAAGCAGCAG 
ATCACTGCAC AGAAACAAGA CCTGGAGGCA GAGGTGTCCA AGCTGACAGG AGAGGTTGCC 
AAGCTGTCAA AAGAACTAGA AGATGCCAAG AATGAAATGG CTTCTCTCTC TGCTGTGGTT 
GTTGCACCTT CTGTTTCTAG CAGTGCTGCT GTTCCCCCTG CCCCTCCTCT GCCTGGTGAC 
TCTGGCACTG TTATTCCACC TCCCCCACCC CCACCTCCTC TTCCTGGAGG TGTGGTCCCA 
CCATCCCCTC CTCTGCCTCC AGGTACTTGT ATCCCTCCAC CTCCTCCTTT ACCTGGAGGT 
GCTTGTATAC CCCCTCCCCC CCAGTTGCCT GGCAGTGCTG CCATCCCTCC ACCTCCTCCT 
CTACCTGGAG TTGCTTCCAT CCCCCCACCT CCCCCTTTGC CTGGGGCTAC TGCCATCCCC 
CCACCTCCCC CTTTGCCTGG GGCTACTGCC ATCCCCCCAC CTCCCCCTTT GCCTGGAGGT 
ACAGGTATAC CACCACCACC TCCTCCTTTG CCTGGAAGTG TTGGCGTTCC CCCACCCCCT 
CCCTTGCCTG GAGGACCAGG ACTGCCTCCT CCCCCTCCCC CTTTTCCTGG AGCACCTGGC 
ATTCCTCCAC CTCCACCTGG TATGGGCGTG CCTCCACCTC CCCCCTTTGG ATTTGGGGTT 
CCTGCGGCCC CAGTTCTGCC ATTTGGATTA ACCCCCAAAA AAGTTTATAA GCCAGAGGTG 
CAGCTCCGGA GGCCAAACTG GTCCAAGTTT GTGGCTGAGG ACCTTTCCCA GGACTGCTTC 
TGGACAAAGG TGAAGGAGGA CCGCTTTGAG AACAATGAAC TTTTTGCCAA ACTTACCCTT 
GCCTTCTCCG CCCAGACCAA GACTTCTAAA GTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245128.1
CDS	317..2755
Translation	MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVVVAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLPGSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKVF

Target ORF information:

RefSeq Version	XM_011246826.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 1 (Diaph1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246826.2

ORF Insert Sequence:

ATGGAGCCGT CCGGCGGGGG CCTGGGGCCC GGCCGCGGTA CCCGGGACAA GAAGAAGGGT 
CGGAGCCCGG ATGAGCTGCC TGCGACGGGC GGCGACGGCG GCAAACATAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG GATTAAGAAG GAGAAAGAAA AGCCCAATTC TGCTCATAGA 
AACTCCTCTG CATCGTACGG AGATGACCCC ACTGCTCAGT CATTGCAGGA CATCTCAGAC 
GAGCAAGTTC TTGTCCTCTT TGAGCAGATG CTGGTGGATA TGAACCTGAA TGAGGAGAAG 
CAGCAGCCTT TGCGAGAGAA GGACATTGTC ATCAAGAGGG AGATGGTGTC GCAATATCTG 
CACACTTCCA AGGCTGGCAT GAACCAGAAA GAGAGCTCTA GGTCTGCCAT GATGTACATC 
CAGGAGCTGA GGTCGGGCTT GCGGGATATG CACCTGCTTA GCTGCCTTGA GTCCCTTCGA 
GTCTCTCTCA ACAATAACCC TGTCAGTTGG GTGCAGACAT TTGGTGCTGA GGGCCTAGCC 
TCCTTATTGG ACATCCTCAA ACGACTCCAT GATGAGAAAG AGGAGACTTC TGGAAACTAC 
GACAGCCGAA ACCAGCATGA GATTATCCGC TGTTTGAAGG CTTTCATGAA CAACAAGTTT 
GGAATCAAAA CTATGTTGGA GACGGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT 
CCTGCTGTTC CCAATATGAT GATTGATGCA GCAAAGCTGC TGTCTGCCCT CTGTATCCTG 
CCGCAGCCGG AGGACATGAA TGAACGAGTT CTAGAGGCAA TGACAGAGAG AGCTGAAATG 
GATGAGGTCG AACGCTTCCA GCCACTTCTG GACGGATTAA AAAGTGGGAC CTCTATTGCC 
CTCAAAGTGG GATGCCTACA GCTCATCAAT GCTCTCATCA CTCCAGCTGA GGAACTGGAC 
TTCCGAGTTC ACATCCGAAG TGAGCTGATG CGCCTGGGGC TGCATCAGGT GTTGCAGGAG 
CTTCGAGAGA TTGAAAATGA AGATATGAAA GTACAGCTGT GCGTGTTTGA TGAACAAGGG 
GATGAAGATT TCTTTGATCT GAAGGGACGG CTGGATGATA TCCGCATGGA GATGGATGAC 
TTTGGTGAAG TTTTTCAGAT TATTTTAAAC ACAGTGAAAG ATTCAAAGGC AGAGCCACAC 
TTCCTGTCTA TCTTGCAGCA TCTCCTGTTG GTCCGAAATG ATTATGAAGC CAGGCCACAG 
TACTATAAAC TGATTGAAGA ATGTGTTTCT CAGATAGTTC TACACAAAAA TGGAACTGAT 
CCTGACTTCA AGTGCCGACA CCTGCAGATT GATATTGAGA GATTGGTTGA TCAAATGATT 
GATAAAACAA AGGTGGAAAA ATCTGAGGCC AAAGCTACAG AGCTGGAAAA AAAGTTGGAT 
TCAGAATTAA CAGCGCGGCA CGAGTTACAA GTAGAAATGA AAAAGATGGA AAATGACTTT 
GAGCAGAAAC TTCAGGATCT TCAAGGAGAA AAGGATGCCT TGGATTCTGA AAAGCAGCAG 
ATCACTGCAC AGAAACAAGA CCTGGAGGCA GAGGTGTCCA AGCTGACAGG AGAGGTTGCC 
AAGCTGTCAA AAGAACTAGA AGATGCCAAG AATGAAATGG CTTCTCTCTC TGCTGTGGTT 
GTTGCACCTT CTGTTTCTAG CAGTGCTGCT GTTCCCCCTG CCCCTCCTCT GCCTGGTGAC 
TCTGGCACTG TTATTCCACC TCCCCCACCC CCACCTCCTC TTCCTGGAGG TGTGGTCCCA 
CCATCCCCTC CTCTGCCTCC AGGTACTTGT ATCCCTCCAC CTCCTCCTTT ACCTGGAGGT 
GCTTGTATAC CCCCTCCCCC CCAGTTGCCT GGCAGTGCTG CCATCCCTCC ACCTCCTCCT 
CTACCTGGAG TTGCTTCCAT CCCCCCACCT CCCCCTTTGC CTGGGGCTAC TGCCATCCCC 
CCACCTCCCC CTTTGCCTGG GGCTACTGCC ATCCCCCCAC CTCCCCCTTT GCCTGGAGGT 
ACAGGTATAC CACCACCACC TCCTCCTTTG CCTGGAAGTG TTGGCGTTCC CCCACCCCCT 
CCCTTGCCTG GAGGACCAGG ACTGCCTCCT CCCCCTCCCC CTTTTCCTGG AGCACCTGGC 
ATTCCTCCAC CTCCACCTGG TATGGGCGTG CCTCCACCTC CCCCCTTTGG ATTTGGGGTT 
CCTGCGGCCC CAGTTCTGCC ATTTGGATTA ACCCCCAAAA AAGTTTATAA GCCAGAGGTG 
CAGCTCCGGA GGCCAAACTG GTCCAAGTTT GTGGCTGAGG ACCTTTCCCA GGACTGCTTC 
TGGACAAAGG TGAAGGAGGA CCGCTTTGAG AACAATGAAC TTTTTGCCAA ACTTACCCTT 
GCCTTCTCCG CCCAGACCAA GACTTCTAAA GTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Diaph1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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