Ddb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddb1
Entrez Gene ID	13194
Full Name	damage specific DNA binding protein 1
Synonyms	127kDa,AA408517,p127-Ddb1
General protein information	Preferred Names damage specific DNA binding protein 1 Names DNA damage-binding protein 1 DDB p127 subunit DNA repair protein UV-damaged DNA-binding factor damage-specific DNA-binding protein, DNA repair damaged-DNA recognition protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 A\|19 6.66 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006526639.3	XP_006526702.1	DNA damage-binding protein 1 isoform X1
XM_006526639.2	XP_006526702.1	DNA damage-binding protein 1 isoform X1
NM_015735.2	NP_056550.1	DNA damage-binding protein 1
NM_015735.1	NP_056550.1	DNA damage-binding protein 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddb1	NP_056550.1
Rattus norvegicus (Norway rat)	Ddb1	NP_741992.2
Danio rerio (zebrafish)	ddb1	NP_956920.1
Xenopus tropicalis (tropical clawed frog)	ddb1	XP_002937687.2
	DDB1	XP_001082958.2
Canis lupus familiaris (dog)	DDB1	XP_533275.2
Drosophila melanogaster (fruit fly)	pic	NP_650257.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002472	XP_312466.2
Oryza sativa (rice)	Os05g0592400	NP_001056495.1
Homo sapiens (human)	DDB1	NP_001914.3
Pan troglodytes (chimpanzee)	DDB1	XP_508472.4
Bos taurus (cattle)	DDB1	NP_001073731.1
Gallus gallus (chicken)	DDB1	NP_989547.1
Arabidopsis thaliana (thale cress)	DDB1A	NP_192451.1
Arabidopsis thaliana (thale cress)	DDB1B	NP_193842.1

Gene Ontology
Bibliography

Related articles in PubMed

DDB1-Mediated CRY1 Degradation Promotes FOXO1-Driven Gluconeogenesis in Liver.
Tong X, Zhang D, Charney N, Jin E, VanDommelen K, Stamper K, Gupta N, Saldate J, Yin L
Diabetes66(10)2571-2582(2017 10)

Sirtuin 7-dependent deacetylation of DDB1 regulates the expression of nuclear receptor TR4.
Karim MF, Yoshizawa T, Sobuz SU, Sato Y, Yamagata K
Biochemical and biophysical research communications490(2)423-428(2017 08)

CRL4
Li G, Ji T, Chen J, Fu Y, Hou L, Feng Y, Zhang T, Song T, Zhao J, Endo Y, Lin H, Cai X, Cang Y
Cell reports18(6)1499-1511(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SIRT7 inhibits TR4 degradation by deacetylation of DDB1.
Title: Sirtuin 7-dependent deacetylation of DDB1 regulates the expression of nuclear receptor TR4.

These studies identify CUL4-DDB1 complex as a novel post-translational regulator of stem and progenitor maintenance and differentiation.
Title: The CUL4-DDB1 ubiquitin ligase complex controls adult and embryonic stem cell differentiation and homeostasis.

uncovered a novel biological role for CUL4A-DDB1-CDT2 E3 ligase that regulates molecular circadian behaviors via promoting ubiquitination-dependent degradation of CRY1
Title: CUL4-DDB1-CDT2 E3 Ligase Regulates the Molecular Clock Activity by Promoting Ubiquitination-Dependent Degradation of the Mammalian CRY1.

UCH-L1 disrupts a complex between the DDB1-CUL4 ubiquitin ligase complex.
Title: Ubiquitin hydrolase UCH-L1 destabilizes mTOR complex 1 by antagonizing DDB1-CUL4-mediated ubiquitination of raptor.

In a constructive process, pM27 recruits DDB1 to exploit ubiquitin ligase complexes catalyzing the obstruction of the STAT2-dependent antiviral state of cells to permit viral replication.
Title: Identification of DNA-damage DNA-binding protein 1 as a conditional essential factor for cytomegalovirus replication in interferon-?-stimulated cells.

The following Ddb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21775	NM_015735.2 Latest version!	Mus musculus damage specific DNA binding protein 1 (Ddb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu55227	XM_006526639.3 Latest version!	Mus musculus damage specific DNA binding protein 1 (Ddb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu55227	XM_006526639.2	Mus musculus damage specific DNA binding protein 1 (Ddb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu21775	NM_015735.1	Mus musculus damage specific DNA binding protein 1 (Ddb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddb1 ( NM_015735.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_056550.1 Mus musculus damage specific DNA binding protein 1 (Ddb1), mRNA.

Price & Availability↑

CloneID	OMu21775
Clone ID Related Accession (Same CDS sequence)	NM_015735.2 , NM_015735.1
Accession Version	NM_015735.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	DNA damage-binding protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125093.4, AB026432.1 and AC132247.3. On Jul 21, 2018 this sequence version replaced NM_015735.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7974084.27621.1, AK155020.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTACA ACTACGTCGT AACGGCGCAG AAGCCTACTG CAGTGAACGG CTGTGTGACC 
GGACACTTTA CTTCAGCGGA AGATCTGAAC CTGTTGATTG CTAAAAACAC AAGATTAGAG 
ATCTATGTGG TCACTGCGGA GGGTCTTCGG CCAGTCAAAG AGGTGGGAAT GTACGGGAAG 
ATTGCAGTCA TGGAGCTCTT CAGGCCCAAG GGGGAGAGCA AAGACCTGCT GTTCATCTTG 
ACTGCTAAAT ACAATGCCTG TATCTTGGAG TATAAGCAGA GTGGCGAAAG CATTGACATC 
ATTACAAGAG CCCATGGCAA TGTCCAGGAC CGCATTGGTC GCCCCTCGGA GACTGGCATC 
ATTGGCATCA TTGACCCTGA GTGCCGTATG ATTGGCCTTC GACTCTACGA TGGCCTTTTC 
AAGGTCATTC CACTAGACCG AGACAATAAA GAGCTCAAGG CCTTTAACAT CCGCCTAGAG 
GAACTGCACG TCATTGATGT CAAGTTCCTG TATGGTTGTC AAGCACCTAC CATTTGCTTT 
GTCTACCAGG ATCCTCAGGG GCGGCATGTG AAAACCTATG AGGTGTCTCT TCGAGAGAAG 
GAGTTCAACA AGGGCCCTTG GAAACAGGAG AATGTAGAAG CCGAAGCTTC AATGGTGATA 
GCAGTCCCAG AGCCTTTTGG AGGTGCCATC ATCATTGGAC AGGAATCAAT CACCTATCAT 
AACGGTGATA AATACCTGGC AATTGCTCCT CCTATCATTA AGCAAAGCAC TATTGTGTGC 
CATAACCGGG TGGATCCTAA TGGCTCGCGG TACCTTCTGG GAGATATGGA GGGACGGCTT 
TTCATGCTGC TCTTAGAGAA GGAGGAGCAG ATGGATGGCA CTGTCACCCT CAAGGACCTT 
CGAGTGGAAC TCCTCGGAGA GACCTCCATT GCTGAGTGCC TAACTTATCT TGATAATGGT 
GTTGTATTTG TCGGGTCTCG CCTAGGTGAC TCCCAGCTCG TAAAGCTCAA TGTCGACAGC 
AACGAACAAG GCTCCTATGT AGTGGCCATG GAAACCTTTA CCAATTTAGG ACCCATTGTG 
GATATGTGTG TGGTGGACCT GGAGAGGCAG GGACAGGGTC AGCTGGTCAC TTGCTCTGGA 
GCTTTCAAGG AAGGTTCCTT GCGGATCATC CGGAACGGCA TTGGAATCCA TGAGCATGCC 
AGCATTGACT TACCAGGCAT CAAAGGTTTA TGGCCTCTGC GCTCTGACCC CGGTCGGGAG 
ACGGATGACA CTTTGGTGCT CTCTTTTGTG GGCCAGACAA GAGTTCTCAT GCTAAACGGA 
GAGGAAGTGG AAGAGACAGA ACTGATGGGC TTTGTGGACG ATCAGCAGAC TTTCTTTTGT 
GGCAACGTGG CTCATCAGCA ACTTATCCAG ATCACCTCAG CATCTGTGAG GTTGGTGTCT 
CAAGAGCCCA AAGCTTTGGT CAGCGAGTGG AAAGAGCCTC AGGGCAAGAA CATCAGTGTG 
GCCTCCTGTA ACAGCAGCCA GGTGGTTGTT GCCGTGGGAA GGGCACTATA TTACCTTCAG 
ATCCACCCTC AGGAGCTCCG GCAAATCAGC CACACAGAGA TGGAACATGA AGTGGCATGC 
TTGGATATCA CCCCATTAGG GGACAGCAAT GGCCTCTCCC CACTTTGTGC CATTGGACTT 
TGGACAGACA TCTCAGCTCG TATCCTGAAG CTCCCATCCT TTGAACTACT ACACAAGGAG 
ATGCTGGGTG GAGAGATCAT TCCTCGATCC ATCCTGATGA CCACTTTTGA GAGTAGCCAC 
TACCTCCTTT GTGCCTTGGG AGATGGGGCT CTTTTCTACT TTGGGCTCAA CATCGAGACA 
GGTTTACTGA GTGACCGTAA AAAGGTGACT TTGGGCACCC AGCCCACGGT GTTGAGGACA 
TTCCGTTCTC TTTCTACCAC CAACGTCTTT GCTTGCTCTG ACCGGCCCAC TGTCATCTAT 
AGCAGCAATC ACAAGTTGGT CTTCTCCAAT GTCAACCTCA AGGAGGTGAA CTATATGTGT 
CCTCTCAACT CAGATGGCTA TCCTGACAGT CTGGCATTGG CCAATAACAG CACTCTCACC 
ATTGGCACCA TCGATGAGAT CCAGAAGCTC CATATTCGCA CAGTTCCCCT CTATGAGTCT 
CCCAGGAAGA TCTGCTATCA GGAAGTGTCT CAGTGCTTTG GGGTCCTTTC CAGCCGCATT 
GAAGTCCAAG ATAGCAGTGG AGGCACTACT GCTTTGAGGC CCAGTGCCAG CACCCAGGCC 
CTGTCCAGCA GCGTCAGCTC CAGCAAACTT TTCTCCAGCA GTACGGCTCC TCACGAGACC 
TCCTTTGGAG AAGAAGTGGA GGTGCACAAC CTCCTCATCA TTGACCAGCA CACCTTTGAA 
GTGCTTCACG CCCATCAGTT TCTACAAAAC GAGTATGCCC TCAGCCTGGT CTCCTGCAAG 
TTGGGTAAAG ACCCCAACAC TTACTTCATT GTGGGCACAG CCATGGTATA TCCTGAAGAG 
GCAGAGCCCA AGCAGGGTCG TATTGTGGTC TTCCAGTATT CAGATGGAAA ACTACAGACT 
GTGGCTGAAA AGGAAGTAAA AGGAGCTGTG TACTCTATGG TGGAGTTTAA TGGGAAGCTG 
CTTGCCAGCA TCAATAGCAC GGTGCGGCTC TATGAGTGGA CCACAGAAAA GGAGCTTCGC 
ACTGAGTGTA ATCACTACAA CAACATCATG GCCCTGTACC TGAAGACCAA GGGCGACTTC 
ATCCTGGTGG GCGACCTCAT GCGCTCAGTG CTGCTGCTTG CCTACAAGCC TATGGAAGGA 
AACTTTGAAG AGATTGCCCG AGACTTTAAT CCCAACTGGA TGAGTGCAGT GGAGATCTTG 
GATGATGACA ATTTTCTGGG AGCTGAAAAT GCCTTTAACT TGTTTGTGTG TCAGAAGGAC 
AGTGCTGCCA CCACTGATGA GGAGCGGCAG CACCTACAGG AGGTTGGTCT CTTCCACCTG 
GGCGAGTTTG TCAATGTGTT CTGCCATGGC TCCCTAGTTA TGCAGAATCT GGGCGAGGCT 
TCTACCCCTA CACAGGGCTC GGTGCTCTTT GGCACAGTCA ACGGCATGAT AGGGCTGGTG 
ACCTCGCTGT CAGAGAGCTG GTATAACCTC CTGTTGGACA TGCAGAACCG ACTCAATAAG 
GTCATCAAAA GCGTGGGCAA GATTGAGCAC TCCTTCTGGA GATCCTTTCA CACTGAGCGA 
AAGACAGAAC CAGCCACAGG CTTCATCGAT GGTGATCTGA TTGAAAGTTT CCTAGATATC 
AGCCGCCCTA AGATGCAGGA GGTTGTGGCA AACTTGCAGT ATGATGATGG CAGTGGTATG 
AAGCGGGAGG CAACTGCAGA TGACCTCATC AAAGTCGTGG AGGAGCTAAC TCGGATCCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056550.1
CDS	110..3532
Translation	MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPGRETDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQGKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAIGLWTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDSSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGEASTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH

Target ORF information:

RefSeq Version	NM_015735.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus damage specific DNA binding protein 1 (Ddb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015735.2

ORF Insert Sequence:

ATGTCGTACA ACTACGTCGT AACGGCGCAG AAGCCTACTG CAGTGAACGG CTGTGTGACC 
GGACACTTTA CTTCAGCGGA AGATCTGAAC CTGTTGATTG CTAAAAACAC AAGATTAGAG 
ATCTATGTGG TCACTGCGGA GGGTCTTCGG CCAGTCAAAG AGGTGGGAAT GTACGGGAAG 
ATTGCAGTCA TGGAGCTCTT CAGGCCCAAG GGGGAGAGCA AAGACCTGCT GTTCATCTTG 
ACTGCTAAAT ACAATGCCTG TATCTTGGAG TATAAGCAGA GTGGCGAAAG CATTGACATC 
ATTACAAGAG CCCATGGCAA TGTCCAGGAC CGCATTGGTC GCCCCTCGGA GACTGGCATC 
ATTGGCATCA TTGACCCTGA GTGCCGTATG ATTGGCCTTC GACTCTACGA TGGCCTTTTC 
AAGGTCATTC CACTAGACCG AGACAATAAA GAGCTCAAGG CCTTTAACAT CCGCCTAGAG 
GAACTGCACG TCATTGATGT CAAGTTCCTG TATGGTTGTC AAGCACCTAC CATTTGCTTT 
GTCTACCAGG ATCCTCAGGG GCGGCATGTG AAAACCTATG AGGTGTCTCT TCGAGAGAAG 
GAGTTCAACA AGGGCCCTTG GAAACAGGAG AATGTAGAAG CCGAAGCTTC AATGGTGATA 
GCAGTCCCAG AGCCTTTTGG AGGTGCCATC ATCATTGGAC AGGAATCAAT CACCTATCAT 
AACGGTGATA AATACCTGGC AATTGCTCCT CCTATCATTA AGCAAAGCAC TATTGTGTGC 
CATAACCGGG TGGATCCTAA TGGCTCGCGG TACCTTCTGG GAGATATGGA GGGACGGCTT 
TTCATGCTGC TCTTAGAGAA GGAGGAGCAG ATGGATGGCA CTGTCACCCT CAAGGACCTT 
CGAGTGGAAC TCCTCGGAGA GACCTCCATT GCTGAGTGCC TAACTTATCT TGATAATGGT 
GTTGTATTTG TCGGGTCTCG CCTAGGTGAC TCCCAGCTCG TAAAGCTCAA TGTCGACAGC 
AACGAACAAG GCTCCTATGT AGTGGCCATG GAAACCTTTA CCAATTTAGG ACCCATTGTG 
GATATGTGTG TGGTGGACCT GGAGAGGCAG GGACAGGGTC AGCTGGTCAC TTGCTCTGGA 
GCTTTCAAGG AAGGTTCCTT GCGGATCATC CGGAACGGCA TTGGAATCCA TGAGCATGCC 
AGCATTGACT TACCAGGCAT CAAAGGTTTA TGGCCTCTGC GCTCTGACCC CGGTCGGGAG 
ACGGATGACA CTTTGGTGCT CTCTTTTGTG GGCCAGACAA GAGTTCTCAT GCTAAACGGA 
GAGGAAGTGG AAGAGACAGA ACTGATGGGC TTTGTGGACG ATCAGCAGAC TTTCTTTTGT 
GGCAACGTGG CTCATCAGCA ACTTATCCAG ATCACCTCAG CATCTGTGAG GTTGGTGTCT 
CAAGAGCCCA AAGCTTTGGT CAGCGAGTGG AAAGAGCCTC AGGGCAAGAA CATCAGTGTG 
GCCTCCTGTA ACAGCAGCCA GGTGGTTGTT GCCGTGGGAA GGGCACTATA TTACCTTCAG 
ATCCACCCTC AGGAGCTCCG GCAAATCAGC CACACAGAGA TGGAACATGA AGTGGCATGC 
TTGGATATCA CCCCATTAGG GGACAGCAAT GGCCTCTCCC CACTTTGTGC CATTGGACTT 
TGGACAGACA TCTCAGCTCG TATCCTGAAG CTCCCATCCT TTGAACTACT ACACAAGGAG 
ATGCTGGGTG GAGAGATCAT TCCTCGATCC ATCCTGATGA CCACTTTTGA GAGTAGCCAC 
TACCTCCTTT GTGCCTTGGG AGATGGGGCT CTTTTCTACT TTGGGCTCAA CATCGAGACA 
GGTTTACTGA GTGACCGTAA AAAGGTGACT TTGGGCACCC AGCCCACGGT GTTGAGGACA 
TTCCGTTCTC TTTCTACCAC CAACGTCTTT GCTTGCTCTG ACCGGCCCAC TGTCATCTAT 
AGCAGCAATC ACAAGTTGGT CTTCTCCAAT GTCAACCTCA AGGAGGTGAA CTATATGTGT 
CCTCTCAACT CAGATGGCTA TCCTGACAGT CTGGCATTGG CCAATAACAG CACTCTCACC 
ATTGGCACCA TCGATGAGAT CCAGAAGCTC CATATTCGCA CAGTTCCCCT CTATGAGTCT 
CCCAGGAAGA TCTGCTATCA GGAAGTGTCT CAGTGCTTTG GGGTCCTTTC CAGCCGCATT 
GAAGTCCAAG ATAGCAGTGG AGGCACTACT GCTTTGAGGC CCAGTGCCAG CACCCAGGCC 
CTGTCCAGCA GCGTCAGCTC CAGCAAACTT TTCTCCAGCA GTACGGCTCC TCACGAGACC 
TCCTTTGGAG AAGAAGTGGA GGTGCACAAC CTCCTCATCA TTGACCAGCA CACCTTTGAA 
GTGCTTCACG CCCATCAGTT TCTACAAAAC GAGTATGCCC TCAGCCTGGT CTCCTGCAAG 
TTGGGTAAAG ACCCCAACAC TTACTTCATT GTGGGCACAG CCATGGTATA TCCTGAAGAG 
GCAGAGCCCA AGCAGGGTCG TATTGTGGTC TTCCAGTATT CAGATGGAAA ACTACAGACT 
GTGGCTGAAA AGGAAGTAAA AGGAGCTGTG TACTCTATGG TGGAGTTTAA TGGGAAGCTG 
CTTGCCAGCA TCAATAGCAC GGTGCGGCTC TATGAGTGGA CCACAGAAAA GGAGCTTCGC 
ACTGAGTGTA ATCACTACAA CAACATCATG GCCCTGTACC TGAAGACCAA GGGCGACTTC 
ATCCTGGTGG GCGACCTCAT GCGCTCAGTG CTGCTGCTTG CCTACAAGCC TATGGAAGGA 
AACTTTGAAG AGATTGCCCG AGACTTTAAT CCCAACTGGA TGAGTGCAGT GGAGATCTTG 
GATGATGACA ATTTTCTGGG AGCTGAAAAT GCCTTTAACT TGTTTGTGTG TCAGAAGGAC 
AGTGCTGCCA CCACTGATGA GGAGCGGCAG CACCTACAGG AGGTTGGTCT CTTCCACCTG 
GGCGAGTTTG TCAATGTGTT CTGCCATGGC TCCCTAGTTA TGCAGAATCT GGGCGAGGCT 
TCTACCCCTA CACAGGGCTC GGTGCTCTTT GGCACAGTCA ACGGCATGAT AGGGCTGGTG 
ACCTCGCTGT CAGAGAGCTG GTATAACCTC CTGTTGGACA TGCAGAACCG ACTCAATAAG 
GTCATCAAAA GCGTGGGCAA GATTGAGCAC TCCTTCTGGA GATCCTTTCA CACTGAGCGA 
AAGACAGAAC CAGCCACAGG CTTCATCGAT GGTGATCTGA TTGAAAGTTT CCTAGATATC 
AGCCGCCCTA AGATGCAGGA GGTTGTGGCA AACTTGCAGT ATGATGATGG CAGTGGTATG 
AAGCGGGAGG CAACTGCAGA TGACCTCATC AAAGTCGTGG AGGAGCTAAC TCGGATCCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddb1 ( NM_015735.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_056550.1 Mus musculus damage specific DNA binding protein 1 (Ddb1), mRNA.

Price & Availability↑

CloneID	OMu21775
Clone ID Related Accession (Same CDS sequence)	NM_015735.2 , NM_015735.1
Accession Version	NM_015735.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-16
Organism	Mus musculus(house mouse)
Product	DNA damage-binding protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB026432.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB026432.1, AK154303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCGTACA ACTACGTCGT AACGGCGCAG AAGCCTACTG CAGTGAACGG CTGTGTGACC 
GGACACTTTA CTTCAGCGGA AGATCTGAAC CTGTTGATTG CTAAAAACAC AAGATTAGAG 
ATCTATGTGG TCACTGCGGA GGGTCTTCGG CCAGTCAAAG AGGTGGGAAT GTACGGGAAG 
ATTGCAGTCA TGGAGCTCTT CAGGCCCAAG GGGGAGAGCA AAGACCTGCT GTTCATCTTG 
ACTGCTAAAT ACAATGCCTG TATCTTGGAG TATAAGCAGA GTGGCGAAAG CATTGACATC 
ATTACAAGAG CCCATGGCAA TGTCCAGGAC CGCATTGGTC GCCCCTCGGA GACTGGCATC 
ATTGGCATCA TTGACCCTGA GTGCCGTATG ATTGGCCTTC GACTCTACGA TGGCCTTTTC 
AAGGTCATTC CACTAGACCG AGACAATAAA GAGCTCAAGG CCTTTAACAT CCGCCTAGAG 
GAACTGCACG TCATTGATGT CAAGTTCCTG TATGGTTGTC AAGCACCTAC CATTTGCTTT 
GTCTACCAGG ATCCTCAGGG GCGGCATGTG AAAACCTATG AGGTGTCTCT TCGAGAGAAG 
GAGTTCAACA AGGGCCCTTG GAAACAGGAG AATGTAGAAG CCGAAGCTTC AATGGTGATA 
GCAGTCCCAG AGCCTTTTGG AGGTGCCATC ATCATTGGAC AGGAATCAAT CACCTATCAT 
AACGGTGATA AATACCTGGC AATTGCTCCT CCTATCATTA AGCAAAGCAC TATTGTGTGC 
CATAACCGGG TGGATCCTAA TGGCTCGCGG TACCTTCTGG GAGATATGGA GGGACGGCTT 
TTCATGCTGC TCTTAGAGAA GGAGGAGCAG ATGGATGGCA CTGTCACCCT CAAGGACCTT 
CGAGTGGAAC TCCTCGGAGA GACCTCCATT GCTGAGTGCC TAACTTATCT TGATAATGGT 
GTTGTATTTG TCGGGTCTCG CCTAGGTGAC TCCCAGCTCG TAAAGCTCAA TGTCGACAGC 
AACGAACAAG GCTCCTATGT AGTGGCCATG GAAACCTTTA CCAATTTAGG ACCCATTGTG 
GATATGTGTG TGGTGGACCT GGAGAGGCAG GGACAGGGTC AGCTGGTCAC TTGCTCTGGA 
GCTTTCAAGG AAGGTTCCTT GCGGATCATC CGGAACGGCA TTGGAATCCA TGAGCATGCC 
AGCATTGACT TACCAGGCAT CAAAGGTTTA TGGCCTCTGC GCTCTGACCC CGGTCGGGAG 
ACGGATGACA CTTTGGTGCT CTCTTTTGTG GGCCAGACAA GAGTTCTCAT GCTAAACGGA 
GAGGAAGTGG AAGAGACAGA ACTGATGGGC TTTGTGGACG ATCAGCAGAC TTTCTTTTGT 
GGCAACGTGG CTCATCAGCA ACTTATCCAG ATCACCTCAG CATCTGTGAG GTTGGTGTCT 
CAAGAGCCCA AAGCTTTGGT CAGCGAGTGG AAAGAGCCTC AGGGCAAGAA CATCAGTGTG 
GCCTCCTGTA ACAGCAGCCA GGTGGTTGTT GCCGTGGGAA GGGCACTATA TTACCTTCAG 
ATCCACCCTC AGGAGCTCCG GCAAATCAGC CACACAGAGA TGGAACATGA AGTGGCATGC 
TTGGATATCA CCCCATTAGG GGACAGCAAT GGCCTCTCCC CACTTTGTGC CATTGGACTT 
TGGACAGACA TCTCAGCTCG TATCCTGAAG CTCCCATCCT TTGAACTACT ACACAAGGAG 
ATGCTGGGTG GAGAGATCAT TCCTCGATCC ATCCTGATGA CCACTTTTGA GAGTAGCCAC 
TACCTCCTTT GTGCCTTGGG AGATGGGGCT CTTTTCTACT TTGGGCTCAA CATCGAGACA 
GGTTTACTGA GTGACCGTAA AAAGGTGACT TTGGGCACCC AGCCCACGGT GTTGAGGACA 
TTCCGTTCTC TTTCTACCAC CAACGTCTTT GCTTGCTCTG ACCGGCCCAC TGTCATCTAT 
AGCAGCAATC ACAAGTTGGT CTTCTCCAAT GTCAACCTCA AGGAGGTGAA CTATATGTGT 
CCTCTCAACT CAGATGGCTA TCCTGACAGT CTGGCATTGG CCAATAACAG CACTCTCACC 
ATTGGCACCA TCGATGAGAT CCAGAAGCTC CATATTCGCA CAGTTCCCCT CTATGAGTCT 
CCCAGGAAGA TCTGCTATCA GGAAGTGTCT CAGTGCTTTG GGGTCCTTTC CAGCCGCATT 
GAAGTCCAAG ATAGCAGTGG AGGCACTACT GCTTTGAGGC CCAGTGCCAG CACCCAGGCC 
CTGTCCAGCA GCGTCAGCTC CAGCAAACTT TTCTCCAGCA GTACGGCTCC TCACGAGACC 
TCCTTTGGAG AAGAAGTGGA GGTGCACAAC CTCCTCATCA TTGACCAGCA CACCTTTGAA 
GTGCTTCACG CCCATCAGTT TCTACAAAAC GAGTATGCCC TCAGCCTGGT CTCCTGCAAG 
TTGGGTAAAG ACCCCAACAC TTACTTCATT GTGGGCACAG CCATGGTATA TCCTGAAGAG 
GCAGAGCCCA AGCAGGGTCG TATTGTGGTC TTCCAGTATT CAGATGGAAA ACTACAGACT 
GTGGCTGAAA AGGAAGTAAA AGGAGCTGTG TACTCTATGG TGGAGTTTAA TGGGAAGCTG 
CTTGCCAGCA TCAATAGCAC GGTGCGGCTC TATGAGTGGA CCACAGAAAA GGAGCTTCGC 
ACTGAGTGTA ATCACTACAA CAACATCATG GCCCTGTACC TGAAGACCAA GGGCGACTTC 
ATCCTGGTGG GCGACCTCAT GCGCTCAGTG CTGCTGCTTG CCTACAAGCC TATGGAAGGA 
AACTTTGAAG AGATTGCCCG AGACTTTAAT CCCAACTGGA TGAGTGCAGT GGAGATCTTG 
GATGATGACA ATTTTCTGGG AGCTGAAAAT GCCTTTAACT TGTTTGTGTG TCAGAAGGAC 
AGTGCTGCCA CCACTGATGA GGAGCGGCAG CACCTACAGG AGGTTGGTCT CTTCCACCTG 
GGCGAGTTTG TCAATGTGTT CTGCCATGGC TCCCTAGTTA TGCAGAATCT GGGCGAGGCT 
TCTACCCCTA CACAGGGCTC GGTGCTCTTT GGCACAGTCA ACGGCATGAT AGGGCTGGTG 
ACCTCGCTGT CAGAGAGCTG GTATAACCTC CTGTTGGACA TGCAGAACCG ACTCAATAAG 
GTCATCAAAA GCGTGGGCAA GATTGAGCAC TCCTTCTGGA GATCCTTTCA CACTGAGCGA 
AAGACAGAAC CAGCCACAGG CTTCATCGAT GGTGATCTGA TTGAAAGTTT CCTAGATATC 
AGCCGCCCTA AGATGCAGGA GGTTGTGGCA AACTTGCAGT ATGATGATGG CAGTGGTATG 
AAGCGGGAGG CAACTGCAGA TGACCTCATC AAAGTCGTGG AGGAGCTAAC TCGGATCCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056550.1
CDS	82..3504
Translation	MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPGRETDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQGKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAIGLWTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDSSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGEASTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH

Target ORF information:

RefSeq Version	NM_015735.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus damage specific DNA binding protein 1 (Ddb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015735.1

ORF Insert Sequence:

ATGTCGTACA ACTACGTCGT AACGGCGCAG AAGCCTACTG CAGTGAACGG CTGTGTGACC 
GGACACTTTA CTTCAGCGGA AGATCTGAAC CTGTTGATTG CTAAAAACAC AAGATTAGAG 
ATCTATGTGG TCACTGCGGA GGGTCTTCGG CCAGTCAAAG AGGTGGGAAT GTACGGGAAG 
ATTGCAGTCA TGGAGCTCTT CAGGCCCAAG GGGGAGAGCA AAGACCTGCT GTTCATCTTG 
ACTGCTAAAT ACAATGCCTG TATCTTGGAG TATAAGCAGA GTGGCGAAAG CATTGACATC 
ATTACAAGAG CCCATGGCAA TGTCCAGGAC CGCATTGGTC GCCCCTCGGA GACTGGCATC 
ATTGGCATCA TTGACCCTGA GTGCCGTATG ATTGGCCTTC GACTCTACGA TGGCCTTTTC 
AAGGTCATTC CACTAGACCG AGACAATAAA GAGCTCAAGG CCTTTAACAT CCGCCTAGAG 
GAACTGCACG TCATTGATGT CAAGTTCCTG TATGGTTGTC AAGCACCTAC CATTTGCTTT 
GTCTACCAGG ATCCTCAGGG GCGGCATGTG AAAACCTATG AGGTGTCTCT TCGAGAGAAG 
GAGTTCAACA AGGGCCCTTG GAAACAGGAG AATGTAGAAG CCGAAGCTTC AATGGTGATA 
GCAGTCCCAG AGCCTTTTGG AGGTGCCATC ATCATTGGAC AGGAATCAAT CACCTATCAT 
AACGGTGATA AATACCTGGC AATTGCTCCT CCTATCATTA AGCAAAGCAC TATTGTGTGC 
CATAACCGGG TGGATCCTAA TGGCTCGCGG TACCTTCTGG GAGATATGGA GGGACGGCTT 
TTCATGCTGC TCTTAGAGAA GGAGGAGCAG ATGGATGGCA CTGTCACCCT CAAGGACCTT 
CGAGTGGAAC TCCTCGGAGA GACCTCCATT GCTGAGTGCC TAACTTATCT TGATAATGGT 
GTTGTATTTG TCGGGTCTCG CCTAGGTGAC TCCCAGCTCG TAAAGCTCAA TGTCGACAGC 
AACGAACAAG GCTCCTATGT AGTGGCCATG GAAACCTTTA CCAATTTAGG ACCCATTGTG 
GATATGTGTG TGGTGGACCT GGAGAGGCAG GGACAGGGTC AGCTGGTCAC TTGCTCTGGA 
GCTTTCAAGG AAGGTTCCTT GCGGATCATC CGGAACGGCA TTGGAATCCA TGAGCATGCC 
AGCATTGACT TACCAGGCAT CAAAGGTTTA TGGCCTCTGC GCTCTGACCC CGGTCGGGAG 
ACGGATGACA CTTTGGTGCT CTCTTTTGTG GGCCAGACAA GAGTTCTCAT GCTAAACGGA 
GAGGAAGTGG AAGAGACAGA ACTGATGGGC TTTGTGGACG ATCAGCAGAC TTTCTTTTGT 
GGCAACGTGG CTCATCAGCA ACTTATCCAG ATCACCTCAG CATCTGTGAG GTTGGTGTCT 
CAAGAGCCCA AAGCTTTGGT CAGCGAGTGG AAAGAGCCTC AGGGCAAGAA CATCAGTGTG 
GCCTCCTGTA ACAGCAGCCA GGTGGTTGTT GCCGTGGGAA GGGCACTATA TTACCTTCAG 
ATCCACCCTC AGGAGCTCCG GCAAATCAGC CACACAGAGA TGGAACATGA AGTGGCATGC 
TTGGATATCA CCCCATTAGG GGACAGCAAT GGCCTCTCCC CACTTTGTGC CATTGGACTT 
TGGACAGACA TCTCAGCTCG TATCCTGAAG CTCCCATCCT TTGAACTACT ACACAAGGAG 
ATGCTGGGTG GAGAGATCAT TCCTCGATCC ATCCTGATGA CCACTTTTGA GAGTAGCCAC 
TACCTCCTTT GTGCCTTGGG AGATGGGGCT CTTTTCTACT TTGGGCTCAA CATCGAGACA 
GGTTTACTGA GTGACCGTAA AAAGGTGACT TTGGGCACCC AGCCCACGGT GTTGAGGACA 
TTCCGTTCTC TTTCTACCAC CAACGTCTTT GCTTGCTCTG ACCGGCCCAC TGTCATCTAT 
AGCAGCAATC ACAAGTTGGT CTTCTCCAAT GTCAACCTCA AGGAGGTGAA CTATATGTGT 
CCTCTCAACT CAGATGGCTA TCCTGACAGT CTGGCATTGG CCAATAACAG CACTCTCACC 
ATTGGCACCA TCGATGAGAT CCAGAAGCTC CATATTCGCA CAGTTCCCCT CTATGAGTCT 
CCCAGGAAGA TCTGCTATCA GGAAGTGTCT CAGTGCTTTG GGGTCCTTTC CAGCCGCATT 
GAAGTCCAAG ATAGCAGTGG AGGCACTACT GCTTTGAGGC CCAGTGCCAG CACCCAGGCC 
CTGTCCAGCA GCGTCAGCTC CAGCAAACTT TTCTCCAGCA GTACGGCTCC TCACGAGACC 
TCCTTTGGAG AAGAAGTGGA GGTGCACAAC CTCCTCATCA TTGACCAGCA CACCTTTGAA 
GTGCTTCACG CCCATCAGTT TCTACAAAAC GAGTATGCCC TCAGCCTGGT CTCCTGCAAG 
TTGGGTAAAG ACCCCAACAC TTACTTCATT GTGGGCACAG CCATGGTATA TCCTGAAGAG 
GCAGAGCCCA AGCAGGGTCG TATTGTGGTC TTCCAGTATT CAGATGGAAA ACTACAGACT 
GTGGCTGAAA AGGAAGTAAA AGGAGCTGTG TACTCTATGG TGGAGTTTAA TGGGAAGCTG 
CTTGCCAGCA TCAATAGCAC GGTGCGGCTC TATGAGTGGA CCACAGAAAA GGAGCTTCGC 
ACTGAGTGTA ATCACTACAA CAACATCATG GCCCTGTACC TGAAGACCAA GGGCGACTTC 
ATCCTGGTGG GCGACCTCAT GCGCTCAGTG CTGCTGCTTG CCTACAAGCC TATGGAAGGA 
AACTTTGAAG AGATTGCCCG AGACTTTAAT CCCAACTGGA TGAGTGCAGT GGAGATCTTG 
GATGATGACA ATTTTCTGGG AGCTGAAAAT GCCTTTAACT TGTTTGTGTG TCAGAAGGAC 
AGTGCTGCCA CCACTGATGA GGAGCGGCAG CACCTACAGG AGGTTGGTCT CTTCCACCTG 
GGCGAGTTTG TCAATGTGTT CTGCCATGGC TCCCTAGTTA TGCAGAATCT GGGCGAGGCT 
TCTACCCCTA CACAGGGCTC GGTGCTCTTT GGCACAGTCA ACGGCATGAT AGGGCTGGTG 
ACCTCGCTGT CAGAGAGCTG GTATAACCTC CTGTTGGACA TGCAGAACCG ACTCAATAAG 
GTCATCAAAA GCGTGGGCAA GATTGAGCAC TCCTTCTGGA GATCCTTTCA CACTGAGCGA 
AAGACAGAAC CAGCCACAGG CTTCATCGAT GGTGATCTGA TTGAAAGTTT CCTAGATATC 
AGCCGCCCTA AGATGCAGGA GGTTGTGGCA AACTTGCAGT ATGATGATGG CAGTGGTATG 
AAGCGGGAGG CAACTGCAGA TGACCTCATC AAAGTCGTGG AGGAGCTAAC TCGGATCCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddb1 ( XM_006526639.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526702.1 Mus musculus damage specific DNA binding protein 1 (Ddb1), transcript variant X1, mRNA.

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CloneID	OMu55227
Clone ID Related Accession (Same CDS sequence)	XM_006526639.3 , XM_006526639.2
Accession Version	XM_006526639.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA damage-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTACA ACTACGTCGT AACGGCGCAG AAGCCTACTG CAGTGAACGG CTGTGTGACC 
GGACACTTTA CTTCAGCGGA AGATCTGAAC CTGTTGATTG CTAAAAACAC AAGATTAGAG 
ATCTATGTGG TCACTGCGGA GGGTCTTCGG CCAGTCAAAG AGGTGGGAAT GTACGGGAAG 
ATTGCAGTCA TGGAGCTCTT CAGGCCCAAG GGGGAGAGCA AAGACCTGCT GTTCATCTTG 
ACTGCTAAAT ACAATGCCTG TATCTTGGAG TATAAGCAGA GTGGCGAAAG CATTGACATC 
ATTACAAGAG CCCATGGCAA TGTCCAGGAC CGCATTGGTC GCCCCTCGGA GACTGGCATC 
ATTGGCATCA TTGACCCTGA GTGCCGTATG ATTGGCCTTC GACTCTACGA TGGCCTTTTC 
AAGGTCATTC CACTAGACCG AGACAATAAA GAGCTCAAGG CCTTTAACAT CCGCCTAGAG 
GAACTGCACG TCATTGATGT CAAGTTCCTG TATGGTTGTC AAGCACCTAC CATTTGCTTT 
GTCTACCAGC CTCTTAAAGG TGACTTTGGC AGCCCCAAGA CTGATGATCC TCAGGGGCGG 
CATGTGAAAA CCTATGAGGT GTCTCTTCGA GAGAAGGAGT TCAACAAGGG CCCTTGGAAA 
CAGGAGAATG TAGAAGCCGA AGCTTCAATG GTGATAGCAG TCCCAGAGCC TTTTGGAGGT 
GCCATCATCA TTGGACAGGA ATCAATCACC TATCATAACG GTGATAAATA CCTGGCAATT 
GCTCCTCCTA TCATTAAGCA AAGCACTATT GTGTGCCATA ACCGGGTGGA TCCTAATGGC 
TCGCGGTACC TTCTGGGAGA TATGGAGGGA CGGCTTTTCA TGCTGCTCTT AGAGAAGGAG 
GAGCAGATGG ATGGCACTGT CACCCTCAAG GACCTTCGAG TGGAACTCCT CGGAGAGACC 
TCCATTGCTG AGTGCCTAAC TTATCTTGAT AATGGTGTTG TATTTGTCGG GTCTCGCCTA 
GGTGACTCCC AGCTCGTAAA GCTCAATGTC GACAGCAACG AACAAGGCTC CTATGTAGTG 
GCCATGGAAA CCTTTACCAA TTTAGGACCC ATTGTGGATA TGTGTGTGGT GGACCTGGAG 
AGGCAGGGAC AGGGTCAGCT GGTCACTTGC TCTGGAGCTT TCAAGGAAGG TTCCTTGCGG 
ATCATCCGGA ACGGCATTGG AATCCATGAG CATGCCAGCA TTGACTTACC AGGCATCAAA 
GGTTTATGGC CTCTGCGCTC TGACCCCGGT CGGGAGACGG ATGACACTTT GGTGCTCTCT 
TTTGTGGGCC AGACAAGAGT TCTCATGCTA AACGGAGAGG AAGTGGAAGA GACAGAACTG 
ATGGGCTTTG TGGACGATCA GCAGACTTTC TTTTGTGGCA ACGTGGCTCA TCAGCAACTT 
ATCCAGATCA CCTCAGCATC TGTGAGGTTG GTGTCTCAAG AGCCCAAAGC TTTGGTCAGC 
GAGTGGAAAG AGCCTCAGGG CAAGAACATC AGTGTGGCCT CCTGTAACAG CAGCCAGGTG 
GTTGTTGCCG TGGGAAGGGC ACTATATTAC CTTCAGATCC ACCCTCAGGA GCTCCGGCAA 
ATCAGCCACA CAGAGATGGA ACATGAAGTG GCATGCTTGG ATATCACCCC ATTAGGGGAC 
AGCAATGGCC TCTCCCCACT TTGTGCCATT GGACTTTGGA CAGACATCTC AGCTCGTATC 
CTGAAGCTCC CATCCTTTGA ACTACTACAC AAGGAGATGC TGGGTGGAGA GATCATTCCT 
CGATCCATCC TGATGACCAC TTTTGAGAGT AGCCACTACC TCCTTTGTGC CTTGGGAGAT 
GGGGCTCTTT TCTACTTTGG GCTCAACATC GAGACAGGTT TACTGAGTGA CCGTAAAAAG 
GTGACTTTGG GCACCCAGCC CACGGTGTTG AGGACATTCC GTTCTCTTTC TACCACCAAC 
GTCTTTGCTT GCTCTGACCG GCCCACTGTC ATCTATAGCA GCAATCACAA GTTGGTCTTC 
TCCAATGTCA ACCTCAAGGA GGTGAACTAT ATGTGTCCTC TCAACTCAGA TGGCTATCCT 
GACAGTCTGG CATTGGCCAA TAACAGCACT CTCACCATTG GCACCATCGA TGAGATCCAG 
AAGCTCCATA TTCGCACAGT TCCCCTCTAT GAGTCTCCCA GGAAGATCTG CTATCAGGAA 
GTGTCTCAGT GCTTTGGGGT CCTTTCCAGC CGCATTGAAG TCCAAGATAG CAGTGGAGGC 
ACTACTGCTT TGAGGCCCAG TGCCAGCACC CAGGCCCTGT CCAGCAGCGT CAGCTCCAGC 
AAACTTTTCT CCAGCAGTAC GGCTCCTCAC GAGACCTCCT TTGGAGAAGA AGTGGAGGTG 
CACAACCTCC TCATCATTGA CCAGCACACC TTTGAAGTGC TTCACGCCCA TCAGTTTCTA 
CAAAACGAGT ATGCCCTCAG CCTGGTCTCC TGCAAGTTGG GTAAAGACCC CAACACTTAC 
TTCATTGTGG GCACAGCCAT GGTATATCCT GAAGAGGCAG AGCCCAAGCA GGGTCGTATT 
GTGGTCTTCC AGTATTCAGA TGGAAAACTA CAGACTGTGG CTGAAAAGGA AGTAAAAGGA 
GCTGTGTACT CTATGGTGGA GTTTAATGGG AAGCTGCTTG CCAGCATCAA TAGCACGGTG 
CGGCTCTATG AGTGGACCAC AGAAAAGGAG CTTCGCACTG AGTGTAATCA CTACAACAAC 
ATCATGGCCC TGTACCTGAA GACCAAGGGC GACTTCATCC TGGTGGGCGA CCTCATGCGC 
TCAGTGCTGC TGCTTGCCTA CAAGCCTATG GAAGGAAACT TTGAAGAGAT TGCCCGAGAC 
TTTAATCCCA ACTGGATGAG TGCAGTGGAG ATCTTGGATG ATGACAATTT TCTGGGAGCT 
GAAAATGCCT TTAACTTGTT TGTGTGTCAG AAGGACAGTG CTGCCACCAC TGATGAGGAG 
CGGCAGCACC TACAGGAGGT TGGTCTCTTC CACCTGGGCG AGTTTGTCAA TGTGTTCTGC 
CATGGCTCCC TAGTTATGCA GAATCTGGGC GAGGCTTCTA CCCCTACACA GGGCTCGGTG 
CTCTTTGGCA CAGTCAACGG CATGATAGGG CTGGTGACCT CGCTGTCAGA GAGCTGGTAT 
AACCTCCTGT TGGACATGCA GAACCGACTC AATAAGGTCA TCAAAAGCGT GGGCAAGATT 
GAGCACTCCT TCTGGAGATC CTTTCACACT GAGCGAAAGA CAGAACCAGC CACAGGCTTC 
ATCGATGGTG ATCTGATTGA AAGTTTCCTA GATATCAGCC GCCCTAAGAT GCAGGAGGTT 
GTGGCAAACT TGCAGTATGA TGATGGCAGT GGTATGAAGC GGGAGGCAAC TGCAGATGAC 
CTCATCAAAG TCGTGGAGGA GCTAACTCGG ATCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526702.1
CDS	139..3597
Translation	MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQPLKGDFGSPKTDDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPGRETDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQGKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAIGLWTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDSSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGEASTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH

Target ORF information:

RefSeq Version	XM_006526639.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus damage specific DNA binding protein 1 (Ddb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526639.3

ORF Insert Sequence:

ATGTCGTACA ACTACGTCGT AACGGCGCAG AAGCCTACTG CAGTGAACGG CTGTGTGACC 
GGACACTTTA CTTCAGCGGA AGATCTGAAC CTGTTGATTG CTAAAAACAC AAGATTAGAG 
ATCTATGTGG TCACTGCGGA GGGTCTTCGG CCAGTCAAAG AGGTGGGAAT GTACGGGAAG 
ATTGCAGTCA TGGAGCTCTT CAGGCCCAAG GGGGAGAGCA AAGACCTGCT GTTCATCTTG 
ACTGCTAAAT ACAATGCCTG TATCTTGGAG TATAAGCAGA GTGGCGAAAG CATTGACATC 
ATTACAAGAG CCCATGGCAA TGTCCAGGAC CGCATTGGTC GCCCCTCGGA GACTGGCATC 
ATTGGCATCA TTGACCCTGA GTGCCGTATG ATTGGCCTTC GACTCTACGA TGGCCTTTTC 
AAGGTCATTC CACTAGACCG AGACAATAAA GAGCTCAAGG CCTTTAACAT CCGCCTAGAG 
GAACTGCACG TCATTGATGT CAAGTTCCTG TATGGTTGTC AAGCACCTAC CATTTGCTTT 
GTCTACCAGC CTCTTAAAGG TGACTTTGGC AGCCCCAAGA CTGATGATCC TCAGGGGCGG 
CATGTGAAAA CCTATGAGGT GTCTCTTCGA GAGAAGGAGT TCAACAAGGG CCCTTGGAAA 
CAGGAGAATG TAGAAGCCGA AGCTTCAATG GTGATAGCAG TCCCAGAGCC TTTTGGAGGT 
GCCATCATCA TTGGACAGGA ATCAATCACC TATCATAACG GTGATAAATA CCTGGCAATT 
GCTCCTCCTA TCATTAAGCA AAGCACTATT GTGTGCCATA ACCGGGTGGA TCCTAATGGC 
TCGCGGTACC TTCTGGGAGA TATGGAGGGA CGGCTTTTCA TGCTGCTCTT AGAGAAGGAG 
GAGCAGATGG ATGGCACTGT CACCCTCAAG GACCTTCGAG TGGAACTCCT CGGAGAGACC 
TCCATTGCTG AGTGCCTAAC TTATCTTGAT AATGGTGTTG TATTTGTCGG GTCTCGCCTA 
GGTGACTCCC AGCTCGTAAA GCTCAATGTC GACAGCAACG AACAAGGCTC CTATGTAGTG 
GCCATGGAAA CCTTTACCAA TTTAGGACCC ATTGTGGATA TGTGTGTGGT GGACCTGGAG 
AGGCAGGGAC AGGGTCAGCT GGTCACTTGC TCTGGAGCTT TCAAGGAAGG TTCCTTGCGG 
ATCATCCGGA ACGGCATTGG AATCCATGAG CATGCCAGCA TTGACTTACC AGGCATCAAA 
GGTTTATGGC CTCTGCGCTC TGACCCCGGT CGGGAGACGG ATGACACTTT GGTGCTCTCT 
TTTGTGGGCC AGACAAGAGT TCTCATGCTA AACGGAGAGG AAGTGGAAGA GACAGAACTG 
ATGGGCTTTG TGGACGATCA GCAGACTTTC TTTTGTGGCA ACGTGGCTCA TCAGCAACTT 
ATCCAGATCA CCTCAGCATC TGTGAGGTTG GTGTCTCAAG AGCCCAAAGC TTTGGTCAGC 
GAGTGGAAAG AGCCTCAGGG CAAGAACATC AGTGTGGCCT CCTGTAACAG CAGCCAGGTG 
GTTGTTGCCG TGGGAAGGGC ACTATATTAC CTTCAGATCC ACCCTCAGGA GCTCCGGCAA 
ATCAGCCACA CAGAGATGGA ACATGAAGTG GCATGCTTGG ATATCACCCC ATTAGGGGAC 
AGCAATGGCC TCTCCCCACT TTGTGCCATT GGACTTTGGA CAGACATCTC AGCTCGTATC 
CTGAAGCTCC CATCCTTTGA ACTACTACAC AAGGAGATGC TGGGTGGAGA GATCATTCCT 
CGATCCATCC TGATGACCAC TTTTGAGAGT AGCCACTACC TCCTTTGTGC CTTGGGAGAT 
GGGGCTCTTT TCTACTTTGG GCTCAACATC GAGACAGGTT TACTGAGTGA CCGTAAAAAG 
GTGACTTTGG GCACCCAGCC CACGGTGTTG AGGACATTCC GTTCTCTTTC TACCACCAAC 
GTCTTTGCTT GCTCTGACCG GCCCACTGTC ATCTATAGCA GCAATCACAA GTTGGTCTTC 
TCCAATGTCA ACCTCAAGGA GGTGAACTAT ATGTGTCCTC TCAACTCAGA TGGCTATCCT 
GACAGTCTGG CATTGGCCAA TAACAGCACT CTCACCATTG GCACCATCGA TGAGATCCAG 
AAGCTCCATA TTCGCACAGT TCCCCTCTAT GAGTCTCCCA GGAAGATCTG CTATCAGGAA 
GTGTCTCAGT GCTTTGGGGT CCTTTCCAGC CGCATTGAAG TCCAAGATAG CAGTGGAGGC 
ACTACTGCTT TGAGGCCCAG TGCCAGCACC CAGGCCCTGT CCAGCAGCGT CAGCTCCAGC 
AAACTTTTCT CCAGCAGTAC GGCTCCTCAC GAGACCTCCT TTGGAGAAGA AGTGGAGGTG 
CACAACCTCC TCATCATTGA CCAGCACACC TTTGAAGTGC TTCACGCCCA TCAGTTTCTA 
CAAAACGAGT ATGCCCTCAG CCTGGTCTCC TGCAAGTTGG GTAAAGACCC CAACACTTAC 
TTCATTGTGG GCACAGCCAT GGTATATCCT GAAGAGGCAG AGCCCAAGCA GGGTCGTATT 
GTGGTCTTCC AGTATTCAGA TGGAAAACTA CAGACTGTGG CTGAAAAGGA AGTAAAAGGA 
GCTGTGTACT CTATGGTGGA GTTTAATGGG AAGCTGCTTG CCAGCATCAA TAGCACGGTG 
CGGCTCTATG AGTGGACCAC AGAAAAGGAG CTTCGCACTG AGTGTAATCA CTACAACAAC 
ATCATGGCCC TGTACCTGAA GACCAAGGGC GACTTCATCC TGGTGGGCGA CCTCATGCGC 
TCAGTGCTGC TGCTTGCCTA CAAGCCTATG GAAGGAAACT TTGAAGAGAT TGCCCGAGAC 
TTTAATCCCA ACTGGATGAG TGCAGTGGAG ATCTTGGATG ATGACAATTT TCTGGGAGCT 
GAAAATGCCT TTAACTTGTT TGTGTGTCAG AAGGACAGTG CTGCCACCAC TGATGAGGAG 
CGGCAGCACC TACAGGAGGT TGGTCTCTTC CACCTGGGCG AGTTTGTCAA TGTGTTCTGC 
CATGGCTCCC TAGTTATGCA GAATCTGGGC GAGGCTTCTA CCCCTACACA GGGCTCGGTG 
CTCTTTGGCA CAGTCAACGG CATGATAGGG CTGGTGACCT CGCTGTCAGA GAGCTGGTAT 
AACCTCCTGT TGGACATGCA GAACCGACTC AATAAGGTCA TCAAAAGCGT GGGCAAGATT 
GAGCACTCCT TCTGGAGATC CTTTCACACT GAGCGAAAGA CAGAACCAGC CACAGGCTTC 
ATCGATGGTG ATCTGATTGA AAGTTTCCTA GATATCAGCC GCCCTAAGAT GCAGGAGGTT 
GTGGCAAACT TGCAGTATGA TGATGGCAGT GGTATGAAGC GGGAGGCAAC TGCAGATGAC 
CTCATCAAAG TCGTGGAGGA GCTAACTCGG ATCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddb1 ( XM_006526639.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526702.1 Mus musculus damage specific DNA binding protein 1 (Ddb1), transcript variant X1, mRNA.

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CloneID	OMu55227
Clone ID Related Accession (Same CDS sequence)	XM_006526639.3 , XM_006526639.2
Accession Version	XM_006526639.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA damage-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTACA ACTACGTCGT AACGGCGCAG AAGCCTACTG CAGTGAACGG CTGTGTGACC 
GGACACTTTA CTTCAGCGGA AGATCTGAAC CTGTTGATTG CTAAAAACAC AAGATTAGAG 
ATCTATGTGG TCACTGCGGA GGGTCTTCGG CCAGTCAAAG AGGTGGGAAT GTACGGGAAG 
ATTGCAGTCA TGGAGCTCTT CAGGCCCAAG GGGGAGAGCA AAGACCTGCT GTTCATCTTG 
ACTGCTAAAT ACAATGCCTG TATCTTGGAG TATAAGCAGA GTGGCGAAAG CATTGACATC 
ATTACAAGAG CCCATGGCAA TGTCCAGGAC CGCATTGGTC GCCCCTCGGA GACTGGCATC 
ATTGGCATCA TTGACCCTGA GTGCCGTATG ATTGGCCTTC GACTCTACGA TGGCCTTTTC 
AAGGTCATTC CACTAGACCG AGACAATAAA GAGCTCAAGG CCTTTAACAT CCGCCTAGAG 
GAACTGCACG TCATTGATGT CAAGTTCCTG TATGGTTGTC AAGCACCTAC CATTTGCTTT 
GTCTACCAGC CTCTTAAAGG TGACTTTGGC AGCCCCAAGA CTGATGATCC TCAGGGGCGG 
CATGTGAAAA CCTATGAGGT GTCTCTTCGA GAGAAGGAGT TCAACAAGGG CCCTTGGAAA 
CAGGAGAATG TAGAAGCCGA AGCTTCAATG GTGATAGCAG TCCCAGAGCC TTTTGGAGGT 
GCCATCATCA TTGGACAGGA ATCAATCACC TATCATAACG GTGATAAATA CCTGGCAATT 
GCTCCTCCTA TCATTAAGCA AAGCACTATT GTGTGCCATA ACCGGGTGGA TCCTAATGGC 
TCGCGGTACC TTCTGGGAGA TATGGAGGGA CGGCTTTTCA TGCTGCTCTT AGAGAAGGAG 
GAGCAGATGG ATGGCACTGT CACCCTCAAG GACCTTCGAG TGGAACTCCT CGGAGAGACC 
TCCATTGCTG AGTGCCTAAC TTATCTTGAT AATGGTGTTG TATTTGTCGG GTCTCGCCTA 
GGTGACTCCC AGCTCGTAAA GCTCAATGTC GACAGCAACG AACAAGGCTC CTATGTAGTG 
GCCATGGAAA CCTTTACCAA TTTAGGACCC ATTGTGGATA TGTGTGTGGT GGACCTGGAG 
AGGCAGGGAC AGGGTCAGCT GGTCACTTGC TCTGGAGCTT TCAAGGAAGG TTCCTTGCGG 
ATCATCCGGA ACGGCATTGG AATCCATGAG CATGCCAGCA TTGACTTACC AGGCATCAAA 
GGTTTATGGC CTCTGCGCTC TGACCCCGGT CGGGAGACGG ATGACACTTT GGTGCTCTCT 
TTTGTGGGCC AGACAAGAGT TCTCATGCTA AACGGAGAGG AAGTGGAAGA GACAGAACTG 
ATGGGCTTTG TGGACGATCA GCAGACTTTC TTTTGTGGCA ACGTGGCTCA TCAGCAACTT 
ATCCAGATCA CCTCAGCATC TGTGAGGTTG GTGTCTCAAG AGCCCAAAGC TTTGGTCAGC 
GAGTGGAAAG AGCCTCAGGG CAAGAACATC AGTGTGGCCT CCTGTAACAG CAGCCAGGTG 
GTTGTTGCCG TGGGAAGGGC ACTATATTAC CTTCAGATCC ACCCTCAGGA GCTCCGGCAA 
ATCAGCCACA CAGAGATGGA ACATGAAGTG GCATGCTTGG ATATCACCCC ATTAGGGGAC 
AGCAATGGCC TCTCCCCACT TTGTGCCATT GGACTTTGGA CAGACATCTC AGCTCGTATC 
CTGAAGCTCC CATCCTTTGA ACTACTACAC AAGGAGATGC TGGGTGGAGA GATCATTCCT 
CGATCCATCC TGATGACCAC TTTTGAGAGT AGCCACTACC TCCTTTGTGC CTTGGGAGAT 
GGGGCTCTTT TCTACTTTGG GCTCAACATC GAGACAGGTT TACTGAGTGA CCGTAAAAAG 
GTGACTTTGG GCACCCAGCC CACGGTGTTG AGGACATTCC GTTCTCTTTC TACCACCAAC 
GTCTTTGCTT GCTCTGACCG GCCCACTGTC ATCTATAGCA GCAATCACAA GTTGGTCTTC 
TCCAATGTCA ACCTCAAGGA GGTGAACTAT ATGTGTCCTC TCAACTCAGA TGGCTATCCT 
GACAGTCTGG CATTGGCCAA TAACAGCACT CTCACCATTG GCACCATCGA TGAGATCCAG 
AAGCTCCATA TTCGCACAGT TCCCCTCTAT GAGTCTCCCA GGAAGATCTG CTATCAGGAA 
GTGTCTCAGT GCTTTGGGGT CCTTTCCAGC CGCATTGAAG TCCAAGATAG CAGTGGAGGC 
ACTACTGCTT TGAGGCCCAG TGCCAGCACC CAGGCCCTGT CCAGCAGCGT CAGCTCCAGC 
AAACTTTTCT CCAGCAGTAC GGCTCCTCAC GAGACCTCCT TTGGAGAAGA AGTGGAGGTG 
CACAACCTCC TCATCATTGA CCAGCACACC TTTGAAGTGC TTCACGCCCA TCAGTTTCTA 
CAAAACGAGT ATGCCCTCAG CCTGGTCTCC TGCAAGTTGG GTAAAGACCC CAACACTTAC 
TTCATTGTGG GCACAGCCAT GGTATATCCT GAAGAGGCAG AGCCCAAGCA GGGTCGTATT 
GTGGTCTTCC AGTATTCAGA TGGAAAACTA CAGACTGTGG CTGAAAAGGA AGTAAAAGGA 
GCTGTGTACT CTATGGTGGA GTTTAATGGG AAGCTGCTTG CCAGCATCAA TAGCACGGTG 
CGGCTCTATG AGTGGACCAC AGAAAAGGAG CTTCGCACTG AGTGTAATCA CTACAACAAC 
ATCATGGCCC TGTACCTGAA GACCAAGGGC GACTTCATCC TGGTGGGCGA CCTCATGCGC 
TCAGTGCTGC TGCTTGCCTA CAAGCCTATG GAAGGAAACT TTGAAGAGAT TGCCCGAGAC 
TTTAATCCCA ACTGGATGAG TGCAGTGGAG ATCTTGGATG ATGACAATTT TCTGGGAGCT 
GAAAATGCCT TTAACTTGTT TGTGTGTCAG AAGGACAGTG CTGCCACCAC TGATGAGGAG 
CGGCAGCACC TACAGGAGGT TGGTCTCTTC CACCTGGGCG AGTTTGTCAA TGTGTTCTGC 
CATGGCTCCC TAGTTATGCA GAATCTGGGC GAGGCTTCTA CCCCTACACA GGGCTCGGTG 
CTCTTTGGCA CAGTCAACGG CATGATAGGG CTGGTGACCT CGCTGTCAGA GAGCTGGTAT 
AACCTCCTGT TGGACATGCA GAACCGACTC AATAAGGTCA TCAAAAGCGT GGGCAAGATT 
GAGCACTCCT TCTGGAGATC CTTTCACACT GAGCGAAAGA CAGAACCAGC CACAGGCTTC 
ATCGATGGTG ATCTGATTGA AAGTTTCCTA GATATCAGCC GCCCTAAGAT GCAGGAGGTT 
GTGGCAAACT TGCAGTATGA TGATGGCAGT GGTATGAAGC GGGAGGCAAC TGCAGATGAC 
CTCATCAAAG TCGTGGAGGA GCTAACTCGG ATCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526702.1
CDS	215..3673
Translation	MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQPLKGDFGSPKTDDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPGRETDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQGKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAIGLWTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDSSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGEASTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH

Target ORF information:

RefSeq Version	XM_006526639.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus damage specific DNA binding protein 1 (Ddb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526639.2

ORF Insert Sequence:

ATGTCGTACA ACTACGTCGT AACGGCGCAG AAGCCTACTG CAGTGAACGG CTGTGTGACC 
GGACACTTTA CTTCAGCGGA AGATCTGAAC CTGTTGATTG CTAAAAACAC AAGATTAGAG 
ATCTATGTGG TCACTGCGGA GGGTCTTCGG CCAGTCAAAG AGGTGGGAAT GTACGGGAAG 
ATTGCAGTCA TGGAGCTCTT CAGGCCCAAG GGGGAGAGCA AAGACCTGCT GTTCATCTTG 
ACTGCTAAAT ACAATGCCTG TATCTTGGAG TATAAGCAGA GTGGCGAAAG CATTGACATC 
ATTACAAGAG CCCATGGCAA TGTCCAGGAC CGCATTGGTC GCCCCTCGGA GACTGGCATC 
ATTGGCATCA TTGACCCTGA GTGCCGTATG ATTGGCCTTC GACTCTACGA TGGCCTTTTC 
AAGGTCATTC CACTAGACCG AGACAATAAA GAGCTCAAGG CCTTTAACAT CCGCCTAGAG 
GAACTGCACG TCATTGATGT CAAGTTCCTG TATGGTTGTC AAGCACCTAC CATTTGCTTT 
GTCTACCAGC CTCTTAAAGG TGACTTTGGC AGCCCCAAGA CTGATGATCC TCAGGGGCGG 
CATGTGAAAA CCTATGAGGT GTCTCTTCGA GAGAAGGAGT TCAACAAGGG CCCTTGGAAA 
CAGGAGAATG TAGAAGCCGA AGCTTCAATG GTGATAGCAG TCCCAGAGCC TTTTGGAGGT 
GCCATCATCA TTGGACAGGA ATCAATCACC TATCATAACG GTGATAAATA CCTGGCAATT 
GCTCCTCCTA TCATTAAGCA AAGCACTATT GTGTGCCATA ACCGGGTGGA TCCTAATGGC 
TCGCGGTACC TTCTGGGAGA TATGGAGGGA CGGCTTTTCA TGCTGCTCTT AGAGAAGGAG 
GAGCAGATGG ATGGCACTGT CACCCTCAAG GACCTTCGAG TGGAACTCCT CGGAGAGACC 
TCCATTGCTG AGTGCCTAAC TTATCTTGAT AATGGTGTTG TATTTGTCGG GTCTCGCCTA 
GGTGACTCCC AGCTCGTAAA GCTCAATGTC GACAGCAACG AACAAGGCTC CTATGTAGTG 
GCCATGGAAA CCTTTACCAA TTTAGGACCC ATTGTGGATA TGTGTGTGGT GGACCTGGAG 
AGGCAGGGAC AGGGTCAGCT GGTCACTTGC TCTGGAGCTT TCAAGGAAGG TTCCTTGCGG 
ATCATCCGGA ACGGCATTGG AATCCATGAG CATGCCAGCA TTGACTTACC AGGCATCAAA 
GGTTTATGGC CTCTGCGCTC TGACCCCGGT CGGGAGACGG ATGACACTTT GGTGCTCTCT 
TTTGTGGGCC AGACAAGAGT TCTCATGCTA AACGGAGAGG AAGTGGAAGA GACAGAACTG 
ATGGGCTTTG TGGACGATCA GCAGACTTTC TTTTGTGGCA ACGTGGCTCA TCAGCAACTT 
ATCCAGATCA CCTCAGCATC TGTGAGGTTG GTGTCTCAAG AGCCCAAAGC TTTGGTCAGC 
GAGTGGAAAG AGCCTCAGGG CAAGAACATC AGTGTGGCCT CCTGTAACAG CAGCCAGGTG 
GTTGTTGCCG TGGGAAGGGC ACTATATTAC CTTCAGATCC ACCCTCAGGA GCTCCGGCAA 
ATCAGCCACA CAGAGATGGA ACATGAAGTG GCATGCTTGG ATATCACCCC ATTAGGGGAC 
AGCAATGGCC TCTCCCCACT TTGTGCCATT GGACTTTGGA CAGACATCTC AGCTCGTATC 
CTGAAGCTCC CATCCTTTGA ACTACTACAC AAGGAGATGC TGGGTGGAGA GATCATTCCT 
CGATCCATCC TGATGACCAC TTTTGAGAGT AGCCACTACC TCCTTTGTGC CTTGGGAGAT 
GGGGCTCTTT TCTACTTTGG GCTCAACATC GAGACAGGTT TACTGAGTGA CCGTAAAAAG 
GTGACTTTGG GCACCCAGCC CACGGTGTTG AGGACATTCC GTTCTCTTTC TACCACCAAC 
GTCTTTGCTT GCTCTGACCG GCCCACTGTC ATCTATAGCA GCAATCACAA GTTGGTCTTC 
TCCAATGTCA ACCTCAAGGA GGTGAACTAT ATGTGTCCTC TCAACTCAGA TGGCTATCCT 
GACAGTCTGG CATTGGCCAA TAACAGCACT CTCACCATTG GCACCATCGA TGAGATCCAG 
AAGCTCCATA TTCGCACAGT TCCCCTCTAT GAGTCTCCCA GGAAGATCTG CTATCAGGAA 
GTGTCTCAGT GCTTTGGGGT CCTTTCCAGC CGCATTGAAG TCCAAGATAG CAGTGGAGGC 
ACTACTGCTT TGAGGCCCAG TGCCAGCACC CAGGCCCTGT CCAGCAGCGT CAGCTCCAGC 
AAACTTTTCT CCAGCAGTAC GGCTCCTCAC GAGACCTCCT TTGGAGAAGA AGTGGAGGTG 
CACAACCTCC TCATCATTGA CCAGCACACC TTTGAAGTGC TTCACGCCCA TCAGTTTCTA 
CAAAACGAGT ATGCCCTCAG CCTGGTCTCC TGCAAGTTGG GTAAAGACCC CAACACTTAC 
TTCATTGTGG GCACAGCCAT GGTATATCCT GAAGAGGCAG AGCCCAAGCA GGGTCGTATT 
GTGGTCTTCC AGTATTCAGA TGGAAAACTA CAGACTGTGG CTGAAAAGGA AGTAAAAGGA 
GCTGTGTACT CTATGGTGGA GTTTAATGGG AAGCTGCTTG CCAGCATCAA TAGCACGGTG 
CGGCTCTATG AGTGGACCAC AGAAAAGGAG CTTCGCACTG AGTGTAATCA CTACAACAAC 
ATCATGGCCC TGTACCTGAA GACCAAGGGC GACTTCATCC TGGTGGGCGA CCTCATGCGC 
TCAGTGCTGC TGCTTGCCTA CAAGCCTATG GAAGGAAACT TTGAAGAGAT TGCCCGAGAC 
TTTAATCCCA ACTGGATGAG TGCAGTGGAG ATCTTGGATG ATGACAATTT TCTGGGAGCT 
GAAAATGCCT TTAACTTGTT TGTGTGTCAG AAGGACAGTG CTGCCACCAC TGATGAGGAG 
CGGCAGCACC TACAGGAGGT TGGTCTCTTC CACCTGGGCG AGTTTGTCAA TGTGTTCTGC 
CATGGCTCCC TAGTTATGCA GAATCTGGGC GAGGCTTCTA CCCCTACACA GGGCTCGGTG 
CTCTTTGGCA CAGTCAACGG CATGATAGGG CTGGTGACCT CGCTGTCAGA GAGCTGGTAT 
AACCTCCTGT TGGACATGCA GAACCGACTC AATAAGGTCA TCAAAAGCGT GGGCAAGATT 
GAGCACTCCT TCTGGAGATC CTTTCACACT GAGCGAAAGA CAGAACCAGC CACAGGCTTC 
ATCGATGGTG ATCTGATTGA AAGTTTCCTA GATATCAGCC GCCCTAAGAT GCAGGAGGTT 
GTGGCAAACT TGCAGTATGA TGATGGCAGT GGTATGAAGC GGGAGGCAAC TGCAGATGAC 
CTCATCAAAG TCGTGGAGGA GCTAACTCGG ATCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DDB1-Mediated CRY1 Degradation Promotes FOXO1-Driven Gluconeogenesis in Liver.
Diabetes66(10)2571-2582(2017 10)
Tong X,Zhang D,Charney N,Jin E,VanDommelen K,Stamper K,Gupta N,Saldate J,Yin L

Sirtuin 7-dependent deacetylation of DDB1 regulates the expression of nuclear receptor TR4.
Biochemical and biophysical research communications490(2)423-428(2017 08)
Karim MF,Yoshizawa T,Sobuz SU,Sato Y,Yamagata K

CRL4
Cell reports18(6)1499-1511(2017 Feb)
Li G,Ji T,Chen J,Fu Y,Hou L,Feng Y,Zhang T,Song T,Zhao J,Endo Y,Lin H,Cai X,Cang Y

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Ddb1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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