Cyp51 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp51
Entrez Gene ID	13121
Full Name	cytochrome P450, family 51
Synonyms	AI426508,CYPLI,Cyp51a1,Ldm,P450-14DM,P450LI
General protein information	Preferred Names cytochrome P450, family 51 Names lanosterol 14-alpha demethylase cytochrome P450 51A1 cytochrome P450, 51 cytochrome P450-14DM cytochrome P45014DM cytochrome P450LI sterol 14-alpha demethylase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 A1\|5 2.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_020010.2	NP_064394.2	lanosterol 14-alpha demethylase

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cyp51	NP_037073.1
Danio rerio (zebrafish)	cyp51	NP_001001730.2
Pan troglodytes (chimpanzee)	CYP51A1	XP_001165634.2
Bos taurus (cattle)	CYP51A1	NP_001020490.1
Mus musculus (house mouse)	Cyp51	NP_064394.2
Gallus gallus (chicken)	CYP51A1	NP_001041542.1
Homo sapiens (human)	CYP51A1	NP_000777.1
Eremothecium gossypii	AGOS_ADR162W	NP_984259.1
Schizosaccharomyces pombe (fission yeast)	erg11	NP_592990.1
Canis lupus familiaris (dog)	CYP51A1	XP_532457.3
Saccharomyces cerevisiae (baker's yeast)	ERG11	NP_011871.1
Magnaporthe oryzae (rice blast fungus)	MGG_04628	XP_003710875.1
Arabidopsis thaliana (thale cress)	CYP51G1	NP_172633.1
Oryza sativa (rice)	Os11g0525200	NP_001067991.1
	LOC703490	XP_002803431.1
Kluyveromyces lactis	KLLA0E03653g	XP_454109.1
Xenopus tropicalis (tropical clawed frog)	cyp51a1	NP_001016194.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of CYP51 in the Regulation of T3 and FSH-Induced Steroidogenesis in Female Mice.
Liu J, Tian Y, Ding Y, Heng D, Xu K, Liu W, Zhang C
Endocrinology158(11)3974-3987(2017 Nov)

Lanosterol Modulates TLR4-Mediated Innate Immune Responses in Macrophages.
Araldi E, Fern?ndez-Fuertes M, Canfr?n-Duque A, Tang W, Cline GW, Madrigal-Matute J, Pober JS, Lasunci?n MA, Wu D, Fern?ndez-Hernando C, Su?rez Y
Cell reports19(13)2743-2755(2017 Jun)

Normalization of Hepatic Homeostasis in the
Munkacsi AB, Hammond N, Schneider RT, Senanayake DS, Higaki K, Lagutin K, Bloor SJ, Ory DS, Maue RA, Chen FW, Hernandez-Ono A, Dahlson N, Repa JJ, Ginsberg HN, Ioannou YA, Sturley SL
The Journal of biological chemistry292(11)4395-4410(2017 03)

Disrupting Hepatocyte Cyp51 from Cholesterol Synthesis Leads to Progressive Liver Injury in the Developing Mouse and Decreases RORC Signalling.
Urlep ?, Lorbek G, Per?e M, Jeruc J, Juvan P, Matz-Soja M, Gebhardt R, Bj?rkhem I, Hall JA, Bonneau R, Littman DR, Rozman D
Scientific reports740775(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CYP51 is a mediator of T3 and FSH-induced follicular development.
Title: Role of CYP51 in the Regulation of T3 and FSH-Induced Steroidogenesis in Female Mice.

Our Cyp51 LKO model shows that steatosis appears with dietary fats in the absence of dietary cholesterol and its synthesis, while a block in the cholesterol pathway leads to NASH-like features
Title: Lessons from hepatocyte-specific Cyp51 knockout mice: impaired cholesterol synthesis leads to oval cell-driven liver injury.

germ cell-specific ablation of Cyp51, leading to lack of MAS, did not affect testicular morphology, daily sperm production, or reproductive performance in males.
Title: Male germ cell-specific knockout of cholesterogenic cytochrome P450 lanosterol 14?-demethylase (Cyp51).

Cyp51 knock-out mice provide evidence that Cyp51 is essential for embryogenesis and present a potential animal model for studying ABS syndrome in humans.
Title: Mouse knockout of the cholesterogenic cytochrome P450 lanosterol 14alpha-demethylase (Cyp51) resembles Antley-Bixler syndrome.

Findings suggest that FSH, partially at least, employs CYP51, and therefore the MAS pathway, to initiate oocyte meiosis.
Title: Reducing CYP51 inhibits follicle-stimulating hormone induced resumption of mouse oocyte meiosis in vitro.

The following Cyp51 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp51 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11655	NM_020010.2 Latest version!	Mus musculus cytochrome P450, family 51 (Cyp51), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp51 ( NM_020010.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064394.2 Mus musculus cytochrome P450, family 51 (Cyp51), mRNA.

Price & Availability↑

CloneID	OMu11655
Clone ID Related Accession (Same CDS sequence)	NM_020010.2
Accession Version	NM_020010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Mus musculus(house mouse)
Product	lanosterol 14-alpha demethylase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB524876.1, AK028815.1, AC068609.3 and AC064793.3. On Jul 22, 2005 this sequence version replaced NM_020010.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.187737.1, AK028815.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTACTTC TGGGCTTGCT GCAGTCAGGC GGCTGGGTGC TCGGACAGGC GATGGAGCAG 
GTGACAGGAG GCAACTTGCT TTCCACGCTG CTCATCGCCT GCGCCTTCAC TCTCAGCCTC 
GTCTACCTGT TCCGTCTCGC CGTGGGCCAC ATGGTCCAGC TGCCCGCTGG AGCGAAAAGT 
CCACCACATA TTTACTCTCC AATTCCATTC CTTGGCCATG CAATAGCATT TGGGAAAAGT 
CCAATTGAGT TTCTGGAAAA TGCATATGAG AAGTATGGAC CTGTGTTTAG CTTTACCATG 
GTGGGCAAGA CCTTCACTTA CCTTCTGGGG AGTGATGCAG CTGCGCTGCT CTTCAATAGT 
AAAAACGAAG ACCTGAATGC AGAAGAAGTC TACGGTCGGC TGACAACACC TGTGTTTGGG 
AAGGGAGTTG CATATGATGT GCCAAATGCA ATTTTCTTGG AACAGAAGAA AATCATAAAA 
AGTGGCCTTA ACATAGCCCA CTTTAAGCAG TATGTTCCTA TCATTGAAAA AGAAGCAAAG 
GAATACTTTC AAAGTTGGGG AGAAAGCGGA GAAAGAAATG TGTTTGAAGC ACTGTCTGAG 
CTCATAATTT TGACAGCTAG CCATTGTTTA CATGGAAAGG AAATTAGAAG TCAACTCAAC 
GAGAAGGTGG CTCAGCTGTA CGCAGACCTG GATGGAGGTT TTACCCACGC TGCCTGGCTA 
TTGCCAGCTT GGCTGCCTCT GCCAAGTTTC AGGCGCAGGG ATAGAGCCCA TCGAGAGATC 
AAGAATATTT TCTATAAGGC CATCCAGAAG CGCAGGCTGT CAAAAGAACC AGCTGAGGAC 
ATTCTTCAAA CTTTACTAGA TTCTACATAC AAGGACGGGC GTCCTCTAAC AGATGAGGAG 
ATATCAGGGA TGCTCATCGG ACTGCTGCTG GCTGGACAGC ACACATCCTC CACCACCAGT 
GCCTGGATGG GCTTCTTTCT GGCCAAAGAT AAACCACTTC AAGAAAAATG CTACTTAGAA 
CAGAAAGCAG TGTGTGGCGA GGATCTGCCT CCTTTAACTT ACGACCAGTT GAAGGATCTG 
AATTTACTTG ATAGATGCAT AAAAGAAACA CTAAGACTGA GGCCTCCAAT AATGACCATG 
ATGAGAATGG CCAAGACCCC TCAGACGGTG GCAGGGTACA CCATTCCTCC AGGACATCAG 
GTGTGTGTCT CTCCTACTGT CAATCAAAGA CTGAAAGACT CCTGGGCAGA GCGCTTGGAC 
TTTAATCCTG ACCGCTACTT ACAGGATAAC CCAGCATCAG GAGAGAAGTT TGCCTATGTG 
CCATTTGGAG CTGGGCGTCA TCGTTGTGTT GGAGAAAATT TTGCCTATGT TCAAATTAAG 
ACAATTTGGT CGACTATGCT TCGTTTATAT GAATTTGACC TCATCAATGG ATATTTTCCC 
ACTGTGAATT ATACAACAAT GATCCACACC CCAGAAAACC CAGTAATCCG TTACAAACGA 
CGATCAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064394.2
CDS	391..1902
Translation	MVLLGLLQSGGWVLGQAMEQVTGGNLLSTLLIACAFTLSLVYLFRLAVGHMVQLPAGAKSPPHIYSPIPFLGHAIAFGKSPIEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALLFNSKNEDLNAEEVYGRLTTPVFGKGVAYDVPNAIFLEQKKIIKSGLNIAHFKQYVPIIEKEAKEYFQSWGESGERNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFTHAAWLLPAWLPLPSFRRRDRAHREIKNIFYKAIQKRRLSKEPAEDILQTLLDSTYKDGRPLTDEEISGMLIGLLLAGQHTSSTTSAWMGFFLAKDKPLQEKCYLEQKAVCGEDLPPLTYDQLKDLNLLDRCIKETLRLRPPIMTMMRMAKTPQTVAGYTIPPGHQVCVSPTVNQRLKDSWAERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCVGENFAYVQIKTIWSTMLRLYEFDLINGYFPTVNYTTMIHTPENPVIRYKRRSK

Target ORF information:

RefSeq Version	NM_020010.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome P450, family 51 (Cyp51), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020010.2

ORF Insert Sequence:

ATGGTACTTC TGGGCTTGCT GCAGTCAGGC GGCTGGGTGC TCGGACAGGC GATGGAGCAG 
GTGACAGGAG GCAACTTGCT TTCCACGCTG CTCATCGCCT GCGCCTTCAC TCTCAGCCTC 
GTCTACCTGT TCCGTCTCGC CGTGGGCCAC ATGGTCCAGC TGCCCGCTGG AGCGAAAAGT 
CCACCACATA TTTACTCTCC AATTCCATTC CTTGGCCATG CAATAGCATT TGGGAAAAGT 
CCAATTGAGT TTCTGGAAAA TGCATATGAG AAGTATGGAC CTGTGTTTAG CTTTACCATG 
GTGGGCAAGA CCTTCACTTA CCTTCTGGGG AGTGATGCAG CTGCGCTGCT CTTCAATAGT 
AAAAACGAAG ACCTGAATGC AGAAGAAGTC TACGGTCGGC TGACAACACC TGTGTTTGGG 
AAGGGAGTTG CATATGATGT GCCAAATGCA ATTTTCTTGG AACAGAAGAA AATCATAAAA 
AGTGGCCTTA ACATAGCCCA CTTTAAGCAG TATGTTCCTA TCATTGAAAA AGAAGCAAAG 
GAATACTTTC AAAGTTGGGG AGAAAGCGGA GAAAGAAATG TGTTTGAAGC ACTGTCTGAG 
CTCATAATTT TGACAGCTAG CCATTGTTTA CATGGAAAGG AAATTAGAAG TCAACTCAAC 
GAGAAGGTGG CTCAGCTGTA CGCAGACCTG GATGGAGGTT TTACCCACGC TGCCTGGCTA 
TTGCCAGCTT GGCTGCCTCT GCCAAGTTTC AGGCGCAGGG ATAGAGCCCA TCGAGAGATC 
AAGAATATTT TCTATAAGGC CATCCAGAAG CGCAGGCTGT CAAAAGAACC AGCTGAGGAC 
ATTCTTCAAA CTTTACTAGA TTCTACATAC AAGGACGGGC GTCCTCTAAC AGATGAGGAG 
ATATCAGGGA TGCTCATCGG ACTGCTGCTG GCTGGACAGC ACACATCCTC CACCACCAGT 
GCCTGGATGG GCTTCTTTCT GGCCAAAGAT AAACCACTTC AAGAAAAATG CTACTTAGAA 
CAGAAAGCAG TGTGTGGCGA GGATCTGCCT CCTTTAACTT ACGACCAGTT GAAGGATCTG 
AATTTACTTG ATAGATGCAT AAAAGAAACA CTAAGACTGA GGCCTCCAAT AATGACCATG 
ATGAGAATGG CCAAGACCCC TCAGACGGTG GCAGGGTACA CCATTCCTCC AGGACATCAG 
GTGTGTGTCT CTCCTACTGT CAATCAAAGA CTGAAAGACT CCTGGGCAGA GCGCTTGGAC 
TTTAATCCTG ACCGCTACTT ACAGGATAAC CCAGCATCAG GAGAGAAGTT TGCCTATGTG 
CCATTTGGAG CTGGGCGTCA TCGTTGTGTT GGAGAAAATT TTGCCTATGT TCAAATTAAG 
ACAATTTGGT CGACTATGCT TCGTTTATAT GAATTTGACC TCATCAATGG ATATTTTCCC 
ACTGTGAATT ATACAACAAT GATCCACACC CCAGAAAACC CAGTAATCCG TTACAAACGA 
CGATCAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of CYP51 in the Regulation of T3 and FSH-Induced Steroidogenesis in Female Mice.
Endocrinology158(11)3974-3987(2017 Nov)
Liu J,Tian Y,Ding Y,Heng D,Xu K,Liu W,Zhang C

Lanosterol Modulates TLR4-Mediated Innate Immune Responses in Macrophages.
Cell reports19(13)2743-2755(2017 Jun)
Araldi E,Fern?ndez-Fuertes M,Canfr?n-Duque A,Tang W,Cline GW,Madrigal-Matute J,Pober JS,Lasunci?n MA,Wu D,Fern?ndez-Hernando C,Su?rez Y

Normalization of Hepatic Homeostasis in the
The Journal of biological chemistry292(11)4395-4410(2017 03)
Munkacsi AB,Hammond N,Schneider RT,Senanayake DS,Higaki K,Lagutin K,Bloor SJ,Ory DS,Maue RA,Chen FW,Hernandez-Ono A,Dahlson N,Repa JJ,Ginsberg HN,Ioannou YA,Sturley SL

Disrupting Hepatocyte Cyp51 from Cholesterol Synthesis Leads to Progressive Liver Injury in the Developing Mouse and Decreases RORC Signalling.
Scientific reports740775(2017 Jan)
Urlep ?,Lorbek G,Per?e M,Jeruc J,Juvan P,Matz-Soja M,Gebhardt R,Bj?rkhem I,Hall JA,Bonneau R,Littman DR,Rozman D

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Cyp51 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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