Cyba cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyba
Entrez Gene ID	13057
Full Name	cytochrome b-245, alpha polypeptide
Synonyms	b558,nmf333,p22-phox,p22phox
General protein information	Preferred Names cytochrome b-245, alpha polypeptide Names cytochrome b-245 light chain cytochrome b(558) alpha chain cytochrome b558 subunit alpha cytochrome beta-558 neutrophil cytochrome b 22 kDa polypeptide p22 phagocyte B-cytochrome p22 phox superoxide-generating NADPH oxidase light chain subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_007806.3	NP_031832.2	cytochrome b-245 light chain isoform 1
NM_001301284.1	NP_001288213.1	cytochrome b-245 light chain isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CYBA	NP_000092.2
Pan troglodytes (chimpanzee)	CYBA	XP_523459.1
Mus musculus (house mouse)	Cyba	NP_031832.2
	LOC696748	XP_001089060.1
Bos taurus (cattle)	CYBA	NP_776459.1
Rattus norvegicus (Norway rat)	Cyba	NP_077074.1
Canis lupus familiaris (dog)	CYBA	NP_001093760.1
Danio rerio (zebrafish)	cyba	NP_956873.1
Xenopus tropicalis (tropical clawed frog)	cyba	NP_001016480.1

Gene Ontology
Bibliography

Related articles in PubMed

Increased Expression of p22phox Mediates Airway Hyperresponsiveness in an Experimental Model of Asthma.
Nagaraj C, Haitchi HM, Heinemann A, Howarth PH, Olschewski A, Marsh LM
Antioxidants & redox signaling27(18)1460-1472(2017 Dec)

Hypoxic vascular response and ventilation/perfusion matching in end-stage COPD may depend on p22phox.
Nagaraj C, Tabeling C, Nagy BM, Jain PP, Marsh LM, Papp R, Pienn M, Witzenrath M, Ghanim B, Klepetko W, Weir EK, Heschl S, Kwapiszewska G, Olschewski A, Olschewski H
The European respiratory journal50(1)(2017 Jul)

Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity.
Thomas DC, Clare S, Sowerby JM, Pardo M, Juss JK, Goulding DA, van der Weyden L, Storisteanu D, Prakash A, Esp?li M, Flint S, Lee JC, Hoenderdos K, Kane L, Harcourt K, Mukhopadhyay S, Umrania Y, Antrobus R, Nathan JA, Adams DJ, Bateman A, Choudhary JS, Lyons PA, Condliffe AM, Chilvers ER, Dougan G, Smith KG
The Journal of experimental medicine214(4)1111-1128(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockout of the cytochrome P450 reductase by CRISPR/Cas9 technology (POR(-/-)) in HEK293 cells overexpressing Nox4 or Nox5 did not interfere with ROS production in intact cells. However, POR(-/-) abolished the signal in NADPH-stimulated assays using membrane fractions from the very same cells. Moreover, membranes of rat smooth muscle cells treated with angiotensin II showed an increased NADPH-dependent signal with lucigen
Title: Cytochrome P450 enzymes but not NADPH oxidases are the source of the NADPH-dependent lucigenin chemiluminescence in membrane assays.

Identification of a PPAR-gamma --> NF-kappaB --> p22phox neuroprotective signaling cascade opens a new avenue for protecting the brain against ischemic insult.
Title: PPAR-? Ameliorates Neuronal Apoptosis and Ischemic Brain Injury via Suppressing NF-?B-Driven p22phox Transcription.

p22phox mRNA expression was increased in diet-induced obese (DIO) mice.
Title: Impaired compensation to femoral artery ligation in diet-induced obese mice is primarily mediated via suppression of collateral growth by Nox2 and p47phox.

The present study provides evidence that augmented EGFRtk impairs vascular function by NADPH oxidase-dependent mechanism. Therefore, EGFRtk and oxidative stress should be potential targets to treat vascular dysfunction in TD2.
Title: Augmented EGF receptor tyrosine kinase activity impairs vascular function by NADPH oxidase-dependent mechanism in type 2 diabetic mouse.

Enhanced p22(phox) expression causes vascular dysfunction through ERK1/2 and p38-mitogen-activated protein kinase-dependent mechanisms in male type 2 diabetic mice.
Title: Enhanced p22phox expression impairs vascular function through p38 and ERK1/2 MAP kinase-dependent mechanisms in type 2 diabetic mice.

The following Cyba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu67493	NM_001301284.1 Latest version!	Mus musculus cytochrome b-245, alpha polypeptide (Cyba), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu18730	NM_007806.3 Latest version!	Mus musculus cytochrome b-245, alpha polypeptide (Cyba), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyba ( NM_001301284.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288213.1 Mus musculus cytochrome b-245, alpha polypeptide (Cyba), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67493
Clone ID Related Accession (Same CDS sequence)	NM_001301284.1
Accession Version	NM_001301284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	cytochrome b-245 light chain isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591003.16, AK018713.1 and BC060635.1. On Aug 1, 2014 this sequence version replaced XM_006530649.1. Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region compared to variant . The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BY702812.1, BQ920402.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCAGA TCGAGTGGGC CATGTGGGCC AACGAACAGG CGCTGGCGTC TGGCCTGATT 
CTCATCACTG GGGGCATCGT GGCTACTGCT GGACGTTTCA CACAGTGGTA TTTCGGCGCC 
TACTCTATCG CTGCAGGTGT GCTCATCTGT CTGCTGGAGT ATCCCCGGGG AAAGAGGAAA 
AAGGGGTCCA CCATGGAGCG ATGGTTGTCG GTGCCTGCAG GCTTCCTCCT GGCCACCATC 
CTGGGGACCG TCTGCTTGGC CATTGCCAGT GTGATCTATC TGCTGGCAGC CATCCGAGGT 
GAGCAGTGGA CTCCCATTGA GCCTAAACCC AAGGAGCGGC CACAGGTTGG AGGCACCATC 
AAGCAACCAC CTACCAACCC CCCACCCCGG CCACCCGCAG AGGTCCGAAA GAAGCCGAGT 
GAGGGTGAAG AGGAGGCAGC CTCAGCTGGA GGACCCCAGG TTAACCCAAT GCCAGTGACA 
GATGAGGTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288213.1
CDS	74..568
Translation	MGQIEWAMWANEQALASGLILITGGIVATAGRFTQWYFGAYSIAAGVLICLLEYPRGKRKKGSTMERWLSVPAGFLLATILGTVCLAIASVIYLLAAIRGEQWTPIEPKPKERPQVGGTIKQPPTNPPPRPPAEVRKKPSEGEEEAASAGGPQVNPMPVTDEVV

Target ORF information:

RefSeq Version	NM_001301284.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome b-245, alpha polypeptide (Cyba), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301284.1

ORF Insert Sequence:

ATGGGGCAGA TCGAGTGGGC CATGTGGGCC AACGAACAGG CGCTGGCGTC TGGCCTGATT 
CTCATCACTG GGGGCATCGT GGCTACTGCT GGACGTTTCA CACAGTGGTA TTTCGGCGCC 
TACTCTATCG CTGCAGGTGT GCTCATCTGT CTGCTGGAGT ATCCCCGGGG AAAGAGGAAA 
AAGGGGTCCA CCATGGAGCG ATGGTTGTCG GTGCCTGCAG GCTTCCTCCT GGCCACCATC 
CTGGGGACCG TCTGCTTGGC CATTGCCAGT GTGATCTATC TGCTGGCAGC CATCCGAGGT 
GAGCAGTGGA CTCCCATTGA GCCTAAACCC AAGGAGCGGC CACAGGTTGG AGGCACCATC 
AAGCAACCAC CTACCAACCC CCCACCCCGG CCACCCGCAG AGGTCCGAAA GAAGCCGAGT 
GAGGGTGAAG AGGAGGCAGC CTCAGCTGGA GGACCCCAGG TTAACCCAAT GCCAGTGACA 
GATGAGGTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyba ( NM_007806.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031832.2 Mus musculus cytochrome b-245, alpha polypeptide (Cyba), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18730
Clone ID Related Accession (Same CDS sequence)	NM_007806.3
Accession Version	NM_007806.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	cytochrome b-245 light chain isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY005999.1, AK152414.1 and CF738398.1. On Nov 22, 2007 this sequence version replaced NM_007806.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF738398.1, AK152414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGA TCGAGTGGGC CATGTGGGCC AACGAACAGG CGCTGGCGTC TGGCCTGATT 
CTCATCACTG GGGGCATCGT GGCTACTGCT GGACGTTTCA CACAGTGGTA TTTCGGCGCC 
TACTCTATCG CTGCAGGTGT GCTCATCTGT CTGCTGGAGT ATCCCCGGGG AAAGAGGAAA 
AAGGGGTCCA CCATGGAGCG ATGTGGACAG AAGTACCTGA CCTCTGTGGT GAAGCTTTTC 
GGGCCCCTCA CCAGGAATTA CTACGTCCGG GCTGCCCTCC ACTTCCTGTT GTCGGTGCCT 
GCAGGCTTCC TCCTGGCCAC CATCCTGGGG ACCGTCTGCT TGGCCATTGC CAGTGTGATC 
TATCTGCTGG CAGCCATCCG AGGTGAGCAG TGGACTCCCA TTGAGCCTAA ACCCAAGGAG 
CGGCCACAGG TTGGAGGCAC CATCAAGCAA CCACCTACCA ACCCCCCACC CCGGCCACCC 
GCAGAGGTCC GAAAGAAGCC GAGTGAGGGT GAAGAGGAGG CAGCCTCAGC TGGAGGACCC 
CAGGTTAACC CAATGCCAGT GACAGATGAG GTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031832.2
CDS	74..652
Translation	MGQIEWAMWANEQALASGLILITGGIVATAGRFTQWYFGAYSIAAGVLICLLEYPRGKRKKGSTMERCGQKYLTSVVKLFGPLTRNYYVRAALHFLLSVPAGFLLATILGTVCLAIASVIYLLAAIRGEQWTPIEPKPKERPQVGGTIKQPPTNPPPRPPAEVRKKPSEGEEEAASAGGPQVNPMPVTDEVV

Target ORF information:

RefSeq Version	NM_007806.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome b-245, alpha polypeptide (Cyba), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007806.3

ORF Insert Sequence:

ATGGGGCAGA TCGAGTGGGC CATGTGGGCC AACGAACAGG CGCTGGCGTC TGGCCTGATT 
CTCATCACTG GGGGCATCGT GGCTACTGCT GGACGTTTCA CACAGTGGTA TTTCGGCGCC 
TACTCTATCG CTGCAGGTGT GCTCATCTGT CTGCTGGAGT ATCCCCGGGG AAAGAGGAAA 
AAGGGGTCCA CCATGGAGCG ATGTGGACAG AAGTACCTGA CCTCTGTGGT GAAGCTTTTC 
GGGCCCCTCA CCAGGAATTA CTACGTCCGG GCTGCCCTCC ACTTCCTGTT GTCGGTGCCT 
GCAGGCTTCC TCCTGGCCAC CATCCTGGGG ACCGTCTGCT TGGCCATTGC CAGTGTGATC 
TATCTGCTGG CAGCCATCCG AGGTGAGCAG TGGACTCCCA TTGAGCCTAA ACCCAAGGAG 
CGGCCACAGG TTGGAGGCAC CATCAAGCAA CCACCTACCA ACCCCCCACC CCGGCCACCC 
GCAGAGGTCC GAAAGAAGCC GAGTGAGGGT GAAGAGGAGG CAGCCTCAGC TGGAGGACCC 
CAGGTTAACC CAATGCCAGT GACAGATGAG GTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increased Expression of p22phox Mediates Airway Hyperresponsiveness in an Experimental Model of Asthma.
Antioxidants & redox signaling27(18)1460-1472(2017 Dec)
Nagaraj C,Haitchi HM,Heinemann A,Howarth PH,Olschewski A,Marsh LM

Hypoxic vascular response and ventilation/perfusion matching in end-stage COPD may depend on p22phox.
The European respiratory journal50(1)(2017 Jul)
Nagaraj C,Tabeling C,Nagy BM,Jain PP,Marsh LM,Papp R,Pienn M,Witzenrath M,Ghanim B,Klepetko W,Weir EK,Heschl S,Kwapiszewska G,Olschewski A,Olschewski H

Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity.
The Journal of experimental medicine214(4)1111-1128(2017 Apr)
Thomas DC,Clare S,Sowerby JM,Pardo M,Juss JK,Goulding DA,van der Weyden L,Storisteanu D,Prakash A,Esp?li M,Flint S,Lee JC,Hoenderdos K,Kane L,Harcourt K,Mukhopadhyay S,Umrania Y,Antrobus R,Nathan JA,Adams DJ,Bateman A,Choudhary JS,Lyons PA,Condliffe AM,Chilvers ER,Dougan G,Smith KG

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Cyba cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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