Ctsh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ctsh
Entrez Gene ID	13036
Full Name	cathepsin H
Synonyms	AL022844
General protein information	Preferred Names cathepsin H Names pro-cathepsin H Cat H cathepsin B3 cathepsin BA
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 E3.1\|9 47.4 cM
Summary	This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin H mini, heavy, and light chains. In rat and human, these three chains can associate to form the mature enzyme, which has both aminopeptidase and endopeptidase activities. Homozygous knockout mice for this gene exhibit impaired lung surfactant processing and reduced tumorigenesis in a pancreatic cancer model. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001312649.1	NP_001299578.1	pro-cathepsin H isoform 2
NM_007801.3	NP_031827.2	pro-cathepsin H isoform 1 preproprotein
XM_011242660.1	XP_011240962.1	pro-cathepsin H isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CTSH	XP_001153217.1
Danio rerio (zebrafish)	ctsh	NP_997853.1
	CTSH	XP_001108862.1
Mus musculus (house mouse)	Ctsh	NP_031827.2
Arabidopsis thaliana (thale cress)	ALP	NP_568921.1
Oryza sativa (rice)	Os09g0442300	NP_001063289.1
Bos taurus (cattle)	CTSH	NP_001029557.1
Arabidopsis thaliana (thale cress)	AT3G45310	NP_566880.1
Canis lupus familiaris (dog)	CTSH	XP_536212.3
Homo sapiens (human)	CTSH	NP_004381.2
Rattus norvegicus (Norway rat)	Ctsh	NP_037071.1
Gallus gallus (chicken)	CTSH	XP_001232765.3

Gene Ontology
Bibliography

Related articles in PubMed

STAT3 and STAT6 Signaling Pathways Synergize to Promote Cathepsin Secretion from Macrophages via IRE1? Activation.
Yan D, Wang HW, Bowman RL, Joyce JA
Cell reports16(11)2914-2927(2016 09)

TAILS N-Terminomics and Proteomics Show Protein Degradation Dominates over Proteolytic Processing by Cathepsins in Pancreatic Tumors.
Prudova A, Gocheva V, Auf dem Keller U, Eckhard U, Olson OC, Akkari L, Butler GS, Fortelny N, Lange PF, Mark JC, Joyce JA, Overall CM
Cell reports16(6)1762-1773(2016 08)

Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection.
Zhu W, Tao L, Quick ML, Joyce JA, Qu JM, Luo ZQ
PLoS pathogens11(3)e1004704(2015 Mar)

CTSH regulates ?-cell function and disease progression in newly diagnosed type 1 diabetes patients.
Fl?yel T, Brorsson C, Nielsen LB, Miani M, Bang-Berthelsen CH, Friedrichsen M, Overgaard AJ, Berchtold LA, Wiberg A, Poulsen P, Hansen L, Rosinger S, Boehm BO, Ram R, Nguyen Q, Mehta M, Morahan G, Concannon P, Bergholdt R, Nielsen JH, Reinheckel T, von Herrath M, Vaag A, Eizirik DL, Mortensen HB, St?rling J, Pociot F
Proceedings of the National Academy of Sciences of the United States of America111(28)10305-10(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data provide strong evidence that CTSH is an important regulator of beta-cell function during progression of T1D
Title: CTSH regulates ?-cell function and disease progression in newly diagnosed type 1 diabetes patients.

Cathepsin H knockout mice show reduced brain levels of (Met)enkephalin and galanin.
Title: Cathepsin H functions as an aminopeptidase in secretory vesicles for production of enkephalin and galanin peptide neurotransmitters.

CTSH overexpression in a subset hepatoma may be thyroid hormone receptor dependent and suggests that this overexpression has an important role in hepatoma progression.
Title: Cathepsin H regulated by the thyroid hormone receptors associate with tumor invasion in human hepatoma cells.

cathepsin H is involved in the SP-B production.
Title: Gene targeting of the cysteine peptidase cathepsin H impairs lung surfactant in mice.

In two mouse models of pancreatic islet cancer, deletion of CtsH reduced tumor progression; mechanisms involved impaired angiogenic switching from premalignant state, poor vascularization of tumors, and increased apoptosis.
Title: Deletion of cathepsin H perturbs angiogenic switching, vascularization and growth of tumors in a mouse model of pancreatic islet cell cancer.

The following Ctsh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ctsh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22489	NM_007801.3 Latest version!	Mus musculus cathepsin H (Ctsh), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu67482	NM_001312649.1 Latest version!	Mus musculus cathepsin H (Ctsh), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu67483	XM_011242660.1 Latest version!	Mus musculus cathepsin H (Ctsh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ctsh ( NM_007801.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031827.2 Mus musculus cathepsin H (Ctsh), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22489
Clone ID Related Accession (Same CDS sequence)	NM_007801.3
Accession Version	NM_007801.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	pro-cathepsin H isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC140392.3, BG078538.2, AK160026.1 and DV043764.1. On Aug 12, 2015 this sequence version replaced NM_007801.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK157376.1, U06119.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCTG CGCTGCCGCT GCTGTGCGCT GGGGCCTGGC TGCTGAGTAC TGGGGCCACC 
GCCGAGCTGA CCGTGAACGC CATAGAAAAG TTTCACTTTA AGTCATGGAT GAAACAGCAT 
CAAAAGACGT ACAGCTCGGT GGAGTACAAC CACAGACTGC AGATGTTTGC CAACAACTGG 
AGGAAGATTC AAGCCCACAA CCAGAGGAAC CACACATTTA AAATGGCATT GAACCAGTTT 
TCAGATATGA GCTTTGCTGA AATAAAACAC AAATTCCTTT GGTCAGAGCC TCAGAATTGC 
TCAGCCACCA AAAGTAACTA CCTCCGAGGT ACAGGCCCCT ACCCTTCCTC CATGGACTGG 
AGGAAGAAAG GAAATGTTGT TTCGCCAGTG AAAAATCAGG GGGCCTGTGG CAGCTGCTGG 
ACTTTCTCTA CCACGGGGGC CCTAGAGTCA GCTGTGGCTA TTGCCAGTGG GAAAATGCTG 
TCTTTGGCTG AGCAGCAGCT GGTGGATTGT GCCCAAGCCT TCAACAATCA TGGCTGCAAA 
GGAGGTCTCC CCAGCCAGGC CTTCGAGTAC ATCCTATACA ACAAGGGCAT CATGGAAGAA 
GACAGCTACC CTTACATAGG CAAGGATAGT TCATGCAGAT TCAACCCCCA AAAAGCTGTT 
GCATTCGTCA AGAATGTTGT CAACATCACA CTCAATGACG AGGCTGCAAT GGTTGAGGCT 
GTGGCTCTAT ACAACCCTGT GAGCTTCGCC TTTGAGGTGA CTGAAGATTT TTTGATGTAT 
AAAAGTGGCG TCTACTCCAG TAAATCCTGT CATAAAACTC CAGATAAAGT AAACCATGCA 
GTCCTGGCGG TTGGCTATGG AGAACAGAAT GGATTACTCT ACTGGATTGT GAAAAACTCT 
TGGGGCTCCC AGTGGGGGGA GAATGGGTAC TTCCTCATTG AACGTGGGAA GAACATGTGT 
GGCCTGGCTG CTTGTGCCTC CTATCCCATT CCTCAGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031827.2
CDS	284..1285
Translation	MWAALPLLCAGAWLLSTGATAELTVNAIEKFHFKSWMKQHQKTYSSVEYNHRLQMFANNWRKIQAHNQRNHTFKMALNQFSDMSFAEIKHKFLWSEPQNCSATKSNYLRGTGPYPSSMDWRKKGNVVSPVKNQGACGSCWTFSTTGALESAVAIASGKMLSLAEQQLVDCAQAFNNHGCKGGLPSQAFEYILYNKGIMEEDSYPYIGKDSSCRFNPQKAVAFVKNVVNITLNDEAAMVEAVALYNPVSFAFEVTEDFLMYKSGVYSSKSCHKTPDKVNHAVLAVGYGEQNGLLYWIVKNSWGSQWGENGYFLIERGKNMCGLAACASYPIPQV

Target ORF information:

RefSeq Version	NM_007801.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cathepsin H (Ctsh), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007801.3

ORF Insert Sequence:

ATGTGGGCTG CGCTGCCGCT GCTGTGCGCT GGGGCCTGGC TGCTGAGTAC TGGGGCCACC 
GCCGAGCTGA CCGTGAACGC CATAGAAAAG TTTCACTTTA AGTCATGGAT GAAACAGCAT 
CAAAAGACGT ACAGCTCGGT GGAGTACAAC CACAGACTGC AGATGTTTGC CAACAACTGG 
AGGAAGATTC AAGCCCACAA CCAGAGGAAC CACACATTTA AAATGGCATT GAACCAGTTT 
TCAGATATGA GCTTTGCTGA AATAAAACAC AAATTCCTTT GGTCAGAGCC TCAGAATTGC 
TCAGCCACCA AAAGTAACTA CCTCCGAGGT ACAGGCCCCT ACCCTTCCTC CATGGACTGG 
AGGAAGAAAG GAAATGTTGT TTCGCCAGTG AAAAATCAGG GGGCCTGTGG CAGCTGCTGG 
ACTTTCTCTA CCACGGGGGC CCTAGAGTCA GCTGTGGCTA TTGCCAGTGG GAAAATGCTG 
TCTTTGGCTG AGCAGCAGCT GGTGGATTGT GCCCAAGCCT TCAACAATCA TGGCTGCAAA 
GGAGGTCTCC CCAGCCAGGC CTTCGAGTAC ATCCTATACA ACAAGGGCAT CATGGAAGAA 
GACAGCTACC CTTACATAGG CAAGGATAGT TCATGCAGAT TCAACCCCCA AAAAGCTGTT 
GCATTCGTCA AGAATGTTGT CAACATCACA CTCAATGACG AGGCTGCAAT GGTTGAGGCT 
GTGGCTCTAT ACAACCCTGT GAGCTTCGCC TTTGAGGTGA CTGAAGATTT TTTGATGTAT 
AAAAGTGGCG TCTACTCCAG TAAATCCTGT CATAAAACTC CAGATAAAGT AAACCATGCA 
GTCCTGGCGG TTGGCTATGG AGAACAGAAT GGATTACTCT ACTGGATTGT GAAAAACTCT 
TGGGGCTCCC AGTGGGGGGA GAATGGGTAC TTCCTCATTG AACGTGGGAA GAACATGTGT 
GGCCTGGCTG CTTGTGCCTC CTATCCCATT CCTCAGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctsh ( NM_001312649.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001299578.1 Mus musculus cathepsin H (Ctsh), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67482
Clone ID Related Accession (Same CDS sequence)	NM_001312649.1
Accession Version	NM_001312649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	pro-cathepsin H isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC140392.3, BG078538.2, AK160026.1 and DV043764.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start site, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus compared to isoform 1. It is not known whether this isoform (2) is proteolytically processed in the same manner as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK167498.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGAAA CAGTGGCATT GAACCAGTTT TCAGATATGA GCTTTGCTGA AATAAAACAC 
AAATTCCTTT GGTCAGAGCC TCAGAATTGC TCAGCCACCA AAAGTAACTA CCTCCGAGGT 
ACAGGCCCCT ACCCTTCCTC CATGGACTGG AGGAAGAAAG GAAATGTTGT TTCGCCAGTG 
AAAAATCAGG GGGCCTGTGG CAGCTGCTGG ACTTTCTCTA CCACGGGGGC CCTAGAGTCA 
GCTGTGGCTA TTGCCAGTGG GAAAATGCTG TCTTTGGCTG AGCAGCAGCT GGTGGATTGT 
GCCCAAGCCT TCAACAATCA TGGCTGCAAA GGAGGTCTCC CCAGCCAGGC CTTCGAGTAC 
ATCCTATACA ACAAGGGCAT CATGGAAGAA GACAGCTACC CTTACATAGG CAAGGATAGT 
TCATGCAGAT TCAACCCCCA AAAAGCTGTT GCATTCGTCA AGAATGTTGT CAACATCACA 
CTCAATGACG AGGCTGCAAT GGTTGAGGCT GTGGCTCTAT ACAACCCTGT GAGCTTCGCC 
TTTGAGGTGA CTGAAGATTT TTTGATGTAT AAAAGTGGCG TCTACTCCAG TAAATCCTGT 
CATAAAACTC CAGATAAAGT AAACCATGCA GTCCTGGCGG TTGGCTATGG AGAACAGAAT 
GGATTACTCT ACTGGATTGT GAAAAACTCT TGGGGCTCCC AGTGGGGGGA GAATGGGTAC 
TTCCTCATTG AACGTGGGAA GAACATGTGT GGCCTGGCTG CTTGTGCCTC CTATCCCATT 
CCTCAGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299578.1
CDS	388..1179
Translation	MDETVALNQFSDMSFAEIKHKFLWSEPQNCSATKSNYLRGTGPYPSSMDWRKKGNVVSPVKNQGACGSCWTFSTTGALESAVAIASGKMLSLAEQQLVDCAQAFNNHGCKGGLPSQAFEYILYNKGIMEEDSYPYIGKDSSCRFNPQKAVAFVKNVVNITLNDEAAMVEAVALYNPVSFAFEVTEDFLMYKSGVYSSKSCHKTPDKVNHAVLAVGYGEQNGLLYWIVKNSWGSQWGENGYFLIERGKNMCGLAACASYPIPQV

Target ORF information:

RefSeq Version	NM_001312649.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cathepsin H (Ctsh), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312649.1

ORF Insert Sequence:

ATGGATGAAA CAGTGGCATT GAACCAGTTT TCAGATATGA GCTTTGCTGA AATAAAACAC 
AAATTCCTTT GGTCAGAGCC TCAGAATTGC TCAGCCACCA AAAGTAACTA CCTCCGAGGT 
ACAGGCCCCT ACCCTTCCTC CATGGACTGG AGGAAGAAAG GAAATGTTGT TTCGCCAGTG 
AAAAATCAGG GGGCCTGTGG CAGCTGCTGG ACTTTCTCTA CCACGGGGGC CCTAGAGTCA 
GCTGTGGCTA TTGCCAGTGG GAAAATGCTG TCTTTGGCTG AGCAGCAGCT GGTGGATTGT 
GCCCAAGCCT TCAACAATCA TGGCTGCAAA GGAGGTCTCC CCAGCCAGGC CTTCGAGTAC 
ATCCTATACA ACAAGGGCAT CATGGAAGAA GACAGCTACC CTTACATAGG CAAGGATAGT 
TCATGCAGAT TCAACCCCCA AAAAGCTGTT GCATTCGTCA AGAATGTTGT CAACATCACA 
CTCAATGACG AGGCTGCAAT GGTTGAGGCT GTGGCTCTAT ACAACCCTGT GAGCTTCGCC 
TTTGAGGTGA CTGAAGATTT TTTGATGTAT AAAAGTGGCG TCTACTCCAG TAAATCCTGT 
CATAAAACTC CAGATAAAGT AAACCATGCA GTCCTGGCGG TTGGCTATGG AGAACAGAAT 
GGATTACTCT ACTGGATTGT GAAAAACTCT TGGGGCTCCC AGTGGGGGGA GAATGGGTAC 
TTCCTCATTG AACGTGGGAA GAACATGTGT GGCCTGGCTG CTTGTGCCTC CTATCCCATT 
CCTCAGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctsh ( XM_011242660.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240962.1 Mus musculus cathepsin H (Ctsh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67483
Clone ID Related Accession (Same CDS sequence)	XM_011242660.1
Accession Version	XM_011242660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pro-cathepsin H isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTG CGCTGCCGCT GCTGTGCGCT GGGGCCTGGC TGCTGAGTAC TGGGGCCACC 
GCCGAGCTGA CCGTGAACGC CATAGAAAAG TTTCACTTTA AGTCATGGAT GAAACAGCAT 
CAAAAGACGT ACAGCTCGGT GGAGTACAAC CACAGACTGC AGATGTTTGC CAACAACTGG 
AGGAAGATTC AAGCCCACAA CCAGAGGAAC CACACATTTA AAATGGCATT GAACCAGTTT 
TCAGATATGA GCTTTGCTGA AATAAAACAC AAATTCCTTT GGTCAGAGCC TCAGAATTGC 
TCAGCCACCA AAAGTAACTA CCTCCGAGGT ACAGGCCCCT ACCCTTCCTC CATGGACTGG 
AGGAAGAAAG GAAATGTTGT TTCGCCAGTG AAAAATCAGG GGGCCTGTGG CAGCTGCTGG 
ACTTTCTCTA CCACGGGGGC CCTAGAGTCA GCTGTGGCTA TTGCCAGTGG GAAAATGCTG 
TCTTTGGCTG AGCAGCAGCT GGTGGATTGT GCCCAAGCCT TCAACAATCA TGGCTGCAAA 
GGAGGTCTCC CCAGCCAGGC CTTCGAGTAC ATCCTATACA ACAAGGGCAT CATGGAAGAA 
GACAGCTACC CTTACATAGG CAAGGATAGT TCATGCAGAT TCAACCCCCA AAAAGCTGTT 
GCATTCGTCA AGAATGTTGT CAACATCACA CTCTCGTTCA ACAGCACCCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240962.1
CDS	251..964
Translation	MWAALPLLCAGAWLLSTGATAELTVNAIEKFHFKSWMKQHQKTYSSVEYNHRLQMFANNWRKIQAHNQRNHTFKMALNQFSDMSFAEIKHKFLWSEPQNCSATKSNYLRGTGPYPSSMDWRKKGNVVSPVKNQGACGSCWTFSTTGALESAVAIASGKMLSLAEQQLVDCAQAFNNHGCKGGLPSQAFEYILYNKGIMEEDSYPYIGKDSSCRFNPQKAVAFVKNVVNITLSFNSTP

Target ORF information:

RefSeq Version	XM_011242660.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cathepsin H (Ctsh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242660.1

ORF Insert Sequence:

ATGTGGGCTG CGCTGCCGCT GCTGTGCGCT GGGGCCTGGC TGCTGAGTAC TGGGGCCACC 
GCCGAGCTGA CCGTGAACGC CATAGAAAAG TTTCACTTTA AGTCATGGAT GAAACAGCAT 
CAAAAGACGT ACAGCTCGGT GGAGTACAAC CACAGACTGC AGATGTTTGC CAACAACTGG 
AGGAAGATTC AAGCCCACAA CCAGAGGAAC CACACATTTA AAATGGCATT GAACCAGTTT 
TCAGATATGA GCTTTGCTGA AATAAAACAC AAATTCCTTT GGTCAGAGCC TCAGAATTGC 
TCAGCCACCA AAAGTAACTA CCTCCGAGGT ACAGGCCCCT ACCCTTCCTC CATGGACTGG 
AGGAAGAAAG GAAATGTTGT TTCGCCAGTG AAAAATCAGG GGGCCTGTGG CAGCTGCTGG 
ACTTTCTCTA CCACGGGGGC CCTAGAGTCA GCTGTGGCTA TTGCCAGTGG GAAAATGCTG 
TCTTTGGCTG AGCAGCAGCT GGTGGATTGT GCCCAAGCCT TCAACAATCA TGGCTGCAAA 
GGAGGTCTCC CCAGCCAGGC CTTCGAGTAC ATCCTATACA ACAAGGGCAT CATGGAAGAA 
GACAGCTACC CTTACATAGG CAAGGATAGT TCATGCAGAT TCAACCCCCA AAAAGCTGTT 
GCATTCGTCA AGAATGTTGT CAACATCACA CTCTCGTTCA ACAGCACCCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

STAT3 and STAT6 Signaling Pathways Synergize to Promote Cathepsin Secretion from Macrophages via IRE1? Activation.
Cell reports16(11)2914-2927(2016 09)
Yan D,Wang HW,Bowman RL,Joyce JA

TAILS N-Terminomics and Proteomics Show Protein Degradation Dominates over Proteolytic Processing by Cathepsins in Pancreatic Tumors.
Cell reports16(6)1762-1773(2016 08)
Prudova A,Gocheva V,Auf dem Keller U,Eckhard U,Olson OC,Akkari L,Butler GS,Fortelny N,Lange PF,Mark JC,Joyce JA,Overall CM

Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection.
PLoS pathogens11(3)e1004704(2015 Mar)
Zhu W,Tao L,Quick ML,Joyce JA,Qu JM,Luo ZQ

CTSH regulates ?-cell function and disease progression in newly diagnosed type 1 diabetes patients.
Proceedings of the National Academy of Sciences of the United States of America111(28)10305-10(2014 Jul)
Fl?yel T,Brorsson C,Nielsen LB,Miani M,Bang-Berthelsen CH,Friedrichsen M,Overgaard AJ,Berchtold LA,Wiberg A,Poulsen P,Hansen L,Rosinger S,Boehm BO,Ram R,Nguyen Q,Mehta M,Morahan G,Concannon P,Bergholdt R,Nielsen JH,Reinheckel T,von Herrath M,Vaag A,Eizirik DL,Mortensen HB,St?rling J,Pociot F

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Ctsh cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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