Dnajc5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnajc5
Entrez Gene ID	13002
Full Name	DnaJ heat shock protein family (Hsp40) member C5
Synonyms	2610314I24Rik,AU018536,Csp
General protein information	Preferred Names DnaJ heat shock protein family (Hsp40) member C5 Names dnaJ homolog subfamily C member 5 DnaJ (Hsp40) homolog, subfamily C, member 5 cysteine string protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H4\|2 103.63 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_016775.3	NP_058055.1	dnaJ homolog subfamily C member 5
NM_001271585.1	NP_001258514.1	dnaJ homolog subfamily C member 5
XM_011239923.2	XP_011238225.1	dnaJ homolog subfamily C member 5 isoform X1
XM_011239923.3	XP_011238225.1	dnaJ homolog subfamily C member 5 isoform X1
NM_001271584.1	NP_001258513.1	dnaJ homolog subfamily C member 5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DNAJC5	XP_530321.2
Danio rerio (zebrafish)	dnajc5ab	XP_001338363.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007620	XP_308251.4
	DNAJC5	XP_001084014.1
Canis lupus familiaris (dog)	DNAJC5	XP_005635371.1
Bos taurus (cattle)	DNAJC5	NP_776958.2
Rattus norvegicus (Norway rat)	Dnajc5	NP_077075.1
Gallus gallus (chicken)	DNAJC5	NP_001264937.1
Homo sapiens (human)	DNAJC5	NP_079495.1
Mus musculus (house mouse)	Dnajc5	NP_058055.1
Drosophila melanogaster (fruit fly)	Csp	NP_524213.1
Danio rerio (zebrafish)	dnajc5aa	NP_001002464.1

Gene Ontology
Bibliography

Related articles in PubMed

Neurons Export Extracellular Vesicles Enriched in Cysteine String Protein and Misfolded Protein Cargo.
Deng J, Koutras C, Donnelier J, Alshehri M, Fotouhi M, Girard M, Casha S, McPherson PS, Robbins SM, Braun JEA
Scientific reports7(1)956(2017 Apr)

Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases.
Sambri I, D'Alessio R, Ezhova Y, Giuliano T, Sorrentino NC, Cacace V, De Risi M, Cataldi M, Annunziato L, De Leonibus E, Fraldi A
EMBO molecular medicine9(1)112-132(2017 Jan)

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
Liu X, Jiao Y, Cao Y, Deng N, Ma Y, Hasty KA, Kang A, Chen H, Stuart JM, Gu W
BMC immunology17(1)25(2016 08)

DnaJ/Hsc70 chaperone complexes control the extracellular release of neurodegenerative-associated proteins.
Fontaine SN, Zheng D, Sabbagh JJ, Martin MD, Chaput D, Darling A, Trotter JH, Stothert AR, Nordhues BA, Lussier A, Baker J, Shelton L, Kahn M, Blair LJ, Stevens SM Jr, Dickey CA
The EMBO journal35(14)1537-49(2016 07)

The Golgi S-acylation machinery comprises zDHHC enzymes with major differences in substrate affinity and S-acylation activity.
Lemonidis K, Gorleku OA, Sanchez-Perez MC, Grefen C, Chamberlain LH
Molecular biology of the cell25(24)3870-83(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data show that neurodegenerative processes associated with lysosomal dysfunction may be presynaptically initiated by a concomitant reduction in alpha-synuclein and CSPalpha levels at nerve terminals.
Title: Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases.

CSPalpha has a role in regulating synaptic excitability and neurotransmission by limiting expression of BK channels.
Title: Cysteine string protein limits expression of the large conductance, calcium-activated K? (BK) channel.

The mRNA and protein expression of CSP1 was elevated in 3T3-L1 adipocytes in insulin resistant states caused by high levels of palmitate and chronic insulin exposure.
Title: Cysteine string protein 1 (CSP1) modulates insulin sensitivity by attenuating glucose transporter 4 (GLUT4) vesicle docking with the plasma membrane.

gamma-Synuclein binds synaptic vesicles but is unable to rescue the phenotype of CSPalpha-deficient mice.
Title: Contrasting effects of ?-synuclein and ?-synuclein on the phenotype of cysteine string protein ? (CSP?) null mutant mice suggest distinct function of these proteins in neuronal synapses.

Study shows that cysteine string protein-alpha is required to maintain the size of the releasable pool of synaptic vesicles in motorneurons, presumably by conveying stability to SNAP-25.
Title: Motorneurons require cysteine string protein-? to maintain the readily releasable vesicular pool and synaptic vesicle recycling.

The following Dnajc5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnajc5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20068	NM_001271584.1 Latest version!	Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu20068	NM_001271585.1 Latest version!	Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu20068	NM_016775.3 Latest version!	Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu20068	XM_011239923.3 Latest version!	Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu20068	XM_011239923.2	Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnajc5 ( NM_016775.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058055.1 Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu20068
Clone ID Related Accession (Same CDS sequence)	NM_016775.3 , NM_001271585.1 , XM_011239923.2 , XM_011239923.3 , NM_001271584.1
Accession Version	NM_016775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily C member 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY333630.1, AK029006.1 and AK035288.1. On Oct 19, 2012 this sequence version replaced NM_016775.2. Transcript Variant: This variant (3) lacks an exon in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.65720.1, SRR1660813.83900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACC AGAGGCAGCG CTCACTCTCT ACTTCCGGGG AATCATTATA CCATGTTCTT 
GGACTGGACA AGAATGCAAC CTCAGATGAC ATTAAAAAGT CCTATCGGAA GCTGGCCCTG 
AAGTATCACC CTGACAAGAA CCCTGATAAC CCAGAGGCTG CAGACAAGTT TAAGGAGATT 
AACAACGCAC ACGCCATCCT GACAGACGCC ACGAAAAGAA ACATTTATGA CAAGTATGGC 
TCGCTGGGGC TCTATGTGGC GGAGCAGTTT GGGGAGGAGA ACGTCAACAC CTACTTCGTA 
CTCTCCAGCT GGTGGGCCAA GGCGCTGTTT GTTGTTTGTG GCCTCCTCAC CTGCTGCTAC 
TGCTGCTGCT GTTTGTGCTG TTGCTTTAAC TGCTGCTGTG GGAAATGCAA GCCCAAGGCA 
CCTGAGGGTG AGGAGACAGA ATTCTACGTA TCCCCTGAAG ACTTGGAGGC ACAGCTGCAG 
TCTGATGAAA GGGAGGCTAC AGACACACCG ATCGTCATAC AGCCAGCATC TGCCACAGAG 
ACCACCCAGC TGACAGCTGA CTCCCACCCC AGCTATCACA CCGACGGGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058055.1
CDS	225..821
Translation	MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVVCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

Target ORF information:

RefSeq Version	NM_016775.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016775.3

ORF Insert Sequence:

ATGGCCGACC AGAGGCAGCG CTCACTCTCT ACTTCCGGGG AATCATTATA CCATGTTCTT 
GGACTGGACA AGAATGCAAC CTCAGATGAC ATTAAAAAGT CCTATCGGAA GCTGGCCCTG 
AAGTATCACC CTGACAAGAA CCCTGATAAC CCAGAGGCTG CAGACAAGTT TAAGGAGATT 
AACAACGCAC ACGCCATCCT GACAGACGCC ACGAAAAGAA ACATTTATGA CAAGTATGGC 
TCGCTGGGGC TCTATGTGGC GGAGCAGTTT GGGGAGGAGA ACGTCAACAC CTACTTCGTA 
CTCTCCAGCT GGTGGGCCAA GGCGCTGTTT GTTGTTTGTG GCCTCCTCAC CTGCTGCTAC 
TGCTGCTGCT GTTTGTGCTG TTGCTTTAAC TGCTGCTGTG GGAAATGCAA GCCCAAGGCA 
CCTGAGGGTG AGGAGACAGA ATTCTACGTA TCCCCTGAAG ACTTGGAGGC ACAGCTGCAG 
TCTGATGAAA GGGAGGCTAC AGACACACCG ATCGTCATAC AGCCAGCATC TGCCACAGAG 
ACCACCCAGC TGACAGCTGA CTCCCACCCC AGCTATCACA CCGACGGGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnajc5 ( NM_001271585.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258514.1 Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20068
Clone ID Related Accession (Same CDS sequence)	NM_016775.3 , NM_001271585.1 , XM_011239923.2 , XM_011239923.3 , NM_001271584.1
Accession Version	NM_001271585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily C member 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY333630.1, CB249513.1, AK029006.1 and AK035288.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB249513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACC AGAGGCAGCG CTCACTCTCT ACTTCCGGGG AATCATTATA CCATGTTCTT 
GGACTGGACA AGAATGCAAC CTCAGATGAC ATTAAAAAGT CCTATCGGAA GCTGGCCCTG 
AAGTATCACC CTGACAAGAA CCCTGATAAC CCAGAGGCTG CAGACAAGTT TAAGGAGATT 
AACAACGCAC ACGCCATCCT GACAGACGCC ACGAAAAGAA ACATTTATGA CAAGTATGGC 
TCGCTGGGGC TCTATGTGGC GGAGCAGTTT GGGGAGGAGA ACGTCAACAC CTACTTCGTA 
CTCTCCAGCT GGTGGGCCAA GGCGCTGTTT GTTGTTTGTG GCCTCCTCAC CTGCTGCTAC 
TGCTGCTGCT GTTTGTGCTG TTGCTTTAAC TGCTGCTGTG GGAAATGCAA GCCCAAGGCA 
CCTGAGGGTG AGGAGACAGA ATTCTACGTA TCCCCTGAAG ACTTGGAGGC ACAGCTGCAG 
TCTGATGAAA GGGAGGCTAC AGACACACCG ATCGTCATAC AGCCAGCATC TGCCACAGAG 
ACCACCCAGC TGACAGCTGA CTCCCACCCC AGCTATCACA CCGACGGGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258514.1
CDS	305..901
Translation	MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVVCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

Target ORF information:

RefSeq Version	NM_001271585.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271585.1

ORF Insert Sequence:

ATGGCCGACC AGAGGCAGCG CTCACTCTCT ACTTCCGGGG AATCATTATA CCATGTTCTT 
GGACTGGACA AGAATGCAAC CTCAGATGAC ATTAAAAAGT CCTATCGGAA GCTGGCCCTG 
AAGTATCACC CTGACAAGAA CCCTGATAAC CCAGAGGCTG CAGACAAGTT TAAGGAGATT 
AACAACGCAC ACGCCATCCT GACAGACGCC ACGAAAAGAA ACATTTATGA CAAGTATGGC 
TCGCTGGGGC TCTATGTGGC GGAGCAGTTT GGGGAGGAGA ACGTCAACAC CTACTTCGTA 
CTCTCCAGCT GGTGGGCCAA GGCGCTGTTT GTTGTTTGTG GCCTCCTCAC CTGCTGCTAC 
TGCTGCTGCT GTTTGTGCTG TTGCTTTAAC TGCTGCTGTG GGAAATGCAA GCCCAAGGCA 
CCTGAGGGTG AGGAGACAGA ATTCTACGTA TCCCCTGAAG ACTTGGAGGC ACAGCTGCAG 
TCTGATGAAA GGGAGGCTAC AGACACACCG ATCGTCATAC AGCCAGCATC TGCCACAGAG 
ACCACCCAGC TGACAGCTGA CTCCCACCCC AGCTATCACA CCGACGGGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnajc5 ( NM_001271584.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258513.1 Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20068
Clone ID Related Accession (Same CDS sequence)	NM_016775.3 , NM_001271585.1 , XM_011239923.2 , XM_011239923.3 , NM_001271584.1
Accession Version	NM_001271584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily C member 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY333630.1, BY101584.1, AK029006.1 and AK035288.1. Transcript Variant: This variant (2) lacks an exon in the 5' UTR and contains another compared to variant 1. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.49849.1, BC012268.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACC AGAGGCAGCG CTCACTCTCT ACTTCCGGGG AATCATTATA CCATGTTCTT 
GGACTGGACA AGAATGCAAC CTCAGATGAC ATTAAAAAGT CCTATCGGAA GCTGGCCCTG 
AAGTATCACC CTGACAAGAA CCCTGATAAC CCAGAGGCTG CAGACAAGTT TAAGGAGATT 
AACAACGCAC ACGCCATCCT GACAGACGCC ACGAAAAGAA ACATTTATGA CAAGTATGGC 
TCGCTGGGGC TCTATGTGGC GGAGCAGTTT GGGGAGGAGA ACGTCAACAC CTACTTCGTA 
CTCTCCAGCT GGTGGGCCAA GGCGCTGTTT GTTGTTTGTG GCCTCCTCAC CTGCTGCTAC 
TGCTGCTGCT GTTTGTGCTG TTGCTTTAAC TGCTGCTGTG GGAAATGCAA GCCCAAGGCA 
CCTGAGGGTG AGGAGACAGA ATTCTACGTA TCCCCTGAAG ACTTGGAGGC ACAGCTGCAG 
TCTGATGAAA GGGAGGCTAC AGACACACCG ATCGTCATAC AGCCAGCATC TGCCACAGAG 
ACCACCCAGC TGACAGCTGA CTCCCACCCC AGCTATCACA CCGACGGGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258513.1
CDS	278..874
Translation	MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVVCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

Target ORF information:

RefSeq Version	NM_001271584.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271584.1

ORF Insert Sequence:

ATGGCCGACC AGAGGCAGCG CTCACTCTCT ACTTCCGGGG AATCATTATA CCATGTTCTT 
GGACTGGACA AGAATGCAAC CTCAGATGAC ATTAAAAAGT CCTATCGGAA GCTGGCCCTG 
AAGTATCACC CTGACAAGAA CCCTGATAAC CCAGAGGCTG CAGACAAGTT TAAGGAGATT 
AACAACGCAC ACGCCATCCT GACAGACGCC ACGAAAAGAA ACATTTATGA CAAGTATGGC 
TCGCTGGGGC TCTATGTGGC GGAGCAGTTT GGGGAGGAGA ACGTCAACAC CTACTTCGTA 
CTCTCCAGCT GGTGGGCCAA GGCGCTGTTT GTTGTTTGTG GCCTCCTCAC CTGCTGCTAC 
TGCTGCTGCT GTTTGTGCTG TTGCTTTAAC TGCTGCTGTG GGAAATGCAA GCCCAAGGCA 
CCTGAGGGTG AGGAGACAGA ATTCTACGTA TCCCCTGAAG ACTTGGAGGC ACAGCTGCAG 
TCTGATGAAA GGGAGGCTAC AGACACACCG ATCGTCATAC AGCCAGCATC TGCCACAGAG 
ACCACCCAGC TGACAGCTGA CTCCCACCCC AGCTATCACA CCGACGGGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnajc5 ( XM_011239923.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238225.1 Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20068
Clone ID Related Accession (Same CDS sequence)	NM_016775.3 , NM_001271585.1 , XM_011239923.2 , XM_011239923.3 , NM_001271584.1
Accession Version	XM_011239923.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily C member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACC AGAGGCAGCG CTCACTCTCT ACTTCCGGGG AATCATTATA CCATGTTCTT 
GGACTGGACA AGAATGCAAC CTCAGATGAC ATTAAAAAGT CCTATCGGAA GCTGGCCCTG 
AAGTATCACC CTGACAAGAA CCCTGATAAC CCAGAGGCTG CAGACAAGTT TAAGGAGATT 
AACAACGCAC ACGCCATCCT GACAGACGCC ACGAAAAGAA ACATTTATGA CAAGTATGGC 
TCGCTGGGGC TCTATGTGGC GGAGCAGTTT GGGGAGGAGA ACGTCAACAC CTACTTCGTA 
CTCTCCAGCT GGTGGGCCAA GGCGCTGTTT GTTGTTTGTG GCCTCCTCAC CTGCTGCTAC 
TGCTGCTGCT GTTTGTGCTG TTGCTTTAAC TGCTGCTGTG GGAAATGCAA GCCCAAGGCA 
CCTGAGGGTG AGGAGACAGA ATTCTACGTA TCCCCTGAAG ACTTGGAGGC ACAGCTGCAG 
TCTGATGAAA GGGAGGCTAC AGACACACCG ATCGTCATAC AGCCAGCATC TGCCACAGAG 
ACCACCCAGC TGACAGCTGA CTCCCACCCC AGCTATCACA CCGACGGGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238225.1
CDS	209..805
Translation	MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVVCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

Target ORF information:

RefSeq Version	XM_011239923.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239923.2

ORF Insert Sequence:

ATGGCCGACC AGAGGCAGCG CTCACTCTCT ACTTCCGGGG AATCATTATA CCATGTTCTT 
GGACTGGACA AGAATGCAAC CTCAGATGAC ATTAAAAAGT CCTATCGGAA GCTGGCCCTG 
AAGTATCACC CTGACAAGAA CCCTGATAAC CCAGAGGCTG CAGACAAGTT TAAGGAGATT 
AACAACGCAC ACGCCATCCT GACAGACGCC ACGAAAAGAA ACATTTATGA CAAGTATGGC 
TCGCTGGGGC TCTATGTGGC GGAGCAGTTT GGGGAGGAGA ACGTCAACAC CTACTTCGTA 
CTCTCCAGCT GGTGGGCCAA GGCGCTGTTT GTTGTTTGTG GCCTCCTCAC CTGCTGCTAC 
TGCTGCTGCT GTTTGTGCTG TTGCTTTAAC TGCTGCTGTG GGAAATGCAA GCCCAAGGCA 
CCTGAGGGTG AGGAGACAGA ATTCTACGTA TCCCCTGAAG ACTTGGAGGC ACAGCTGCAG 
TCTGATGAAA GGGAGGCTAC AGACACACCG ATCGTCATAC AGCCAGCATC TGCCACAGAG 
ACCACCCAGC TGACAGCTGA CTCCCACCCC AGCTATCACA CCGACGGGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnajc5 ( XM_011239923.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238225.1 Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant X2, mRNA.

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CloneID	OMu20068
Clone ID Related Accession (Same CDS sequence)	NM_016775.3 , NM_001271585.1 , XM_011239923.2 , XM_011239923.3 , NM_001271584.1
Accession Version	XM_011239923.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily C member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACC AGAGGCAGCG CTCACTCTCT ACTTCCGGGG AATCATTATA CCATGTTCTT 
GGACTGGACA AGAATGCAAC CTCAGATGAC ATTAAAAAGT CCTATCGGAA GCTGGCCCTG 
AAGTATCACC CTGACAAGAA CCCTGATAAC CCAGAGGCTG CAGACAAGTT TAAGGAGATT 
AACAACGCAC ACGCCATCCT GACAGACGCC ACGAAAAGAA ACATTTATGA CAAGTATGGC 
TCGCTGGGGC TCTATGTGGC GGAGCAGTTT GGGGAGGAGA ACGTCAACAC CTACTTCGTA 
CTCTCCAGCT GGTGGGCCAA GGCGCTGTTT GTTGTTTGTG GCCTCCTCAC CTGCTGCTAC 
TGCTGCTGCT GTTTGTGCTG TTGCTTTAAC TGCTGCTGTG GGAAATGCAA GCCCAAGGCA 
CCTGAGGGTG AGGAGACAGA ATTCTACGTA TCCCCTGAAG ACTTGGAGGC ACAGCTGCAG 
TCTGATGAAA GGGAGGCTAC AGACACACCG ATCGTCATAC AGCCAGCATC TGCCACAGAG 
ACCACCCAGC TGACAGCTGA CTCCCACCCC AGCTATCACA CCGACGGGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238225.1
CDS	208..804
Translation	MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVVCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

Target ORF information:

RefSeq Version	XM_011239923.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member C5 (Dnajc5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239923.3

ORF Insert Sequence:

ATGGCCGACC AGAGGCAGCG CTCACTCTCT ACTTCCGGGG AATCATTATA CCATGTTCTT 
GGACTGGACA AGAATGCAAC CTCAGATGAC ATTAAAAAGT CCTATCGGAA GCTGGCCCTG 
AAGTATCACC CTGACAAGAA CCCTGATAAC CCAGAGGCTG CAGACAAGTT TAAGGAGATT 
AACAACGCAC ACGCCATCCT GACAGACGCC ACGAAAAGAA ACATTTATGA CAAGTATGGC 
TCGCTGGGGC TCTATGTGGC GGAGCAGTTT GGGGAGGAGA ACGTCAACAC CTACTTCGTA 
CTCTCCAGCT GGTGGGCCAA GGCGCTGTTT GTTGTTTGTG GCCTCCTCAC CTGCTGCTAC 
TGCTGCTGCT GTTTGTGCTG TTGCTTTAAC TGCTGCTGTG GGAAATGCAA GCCCAAGGCA 
CCTGAGGGTG AGGAGACAGA ATTCTACGTA TCCCCTGAAG ACTTGGAGGC ACAGCTGCAG 
TCTGATGAAA GGGAGGCTAC AGACACACCG ATCGTCATAC AGCCAGCATC TGCCACAGAG 
ACCACCCAGC TGACAGCTGA CTCCCACCCC AGCTATCACA CCGACGGGTT CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neurons Export Extracellular Vesicles Enriched in Cysteine String Protein and Misfolded Protein Cargo.
Scientific reports7(1)956(2017 Apr)
Deng J,Koutras C,Donnelier J,Alshehri M,Fotouhi M,Girard M,Casha S,McPherson PS,Robbins SM,Braun JEA

Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases.
EMBO molecular medicine9(1)112-132(2017 Jan)
Sambri I,D'Alessio R,Ezhova Y,Giuliano T,Sorrentino NC,Cacace V,De Risi M,Cataldi M,Annunziato L,De Leonibus E,Fraldi A

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
BMC immunology17(1)25(2016 08)
Liu X,Jiao Y,Cao Y,Deng N,Ma Y,Hasty KA,Kang A,Chen H,Stuart JM,Gu W

DnaJ/Hsc70 chaperone complexes control the extracellular release of neurodegenerative-associated proteins.
The EMBO journal35(14)1537-49(2016 07)
Fontaine SN,Zheng D,Sabbagh JJ,Martin MD,Chaput D,Darling A,Trotter JH,Stothert AR,Nordhues BA,Lussier A,Baker J,Shelton L,Kahn M,Blair LJ,Stevens SM Jr,Dickey CA

The Golgi S-acylation machinery comprises zDHHC enzymes with major differences in substrate affinity and S-acylation activity.
Molecular biology of the cell25(24)3870-83(2014 Dec)
Lemonidis K,Gorleku OA,Sanchez-Perez MC,Grefen C,Chamberlain LH

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Dnajc5 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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