Cs cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cs
Entrez Gene ID	12974
Full Name	citrate synthase
Synonyms	2610511A05Rik,9030605P22Rik,Ahl4,BB234005,Cis
General protein information	Preferred Names citrate synthase Names citrate synthase, mitochondrial citrate (Si)-synthase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 D3
Summary	The protein encoded by this gene is a central metabolic pathway enzyme, catalyzing the first step of the tricarboxylic acid cycle in which acetyl coenzyme A and oxaloacetate are converted to citrate and coenzyme A. This enzyme is found in nearly all cells capable of oxidative metabolism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_026444.4	NP_080720.1	citrate synthase, mitochondrial precursor
NM_026444.3	NP_080720.1	citrate synthase, mitochondrial precursor
XM_030244861.1	XP_030100721.1	citrate synthase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CS	XP_001153202.1
Bos taurus (cattle)	CS	NP_001038186.1
Rattus norvegicus (Norway rat)	Cs	NP_570111.1
Danio rerio (zebrafish)	LOC100536541	XP_003201261.1
Drosophila melanogaster (fruit fly)	kdn	NP_572319.2
Arabidopsis thaliana (thale cress)	ATCS	NP_850415.1
Oryza sativa (rice)	Os11g0538900	NP_001068031.1
Gallus gallus (chicken)	CS	XP_003644004.2
	LOC709604	XP_001098147.2
Canis lupus familiaris (dog)	CS	XP_531634.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012048	XP_320478.4
Saccharomyces cerevisiae (baker's yeast)	CIT2	NP_009931.1
Mus musculus (house mouse)	Cs	NP_080720.1
Caenorhabditis elegans (roundworm)	cts-1	NP_499264.1
Xenopus tropicalis (tropical clawed frog)	cs	NP_001017313.1
Homo sapiens (human)	CS	NP_004068.2
Danio rerio (zebrafish)	cs	NP_955892.1
Oryza sativa (rice)	Os02g0194100	NP_001046175.1

Gene Ontology
Bibliography

Related articles in PubMed

Cardiac Med1 deletion promotes early lethality, cardiac remodeling, and transcriptional reprogramming.
Spitler KM, Ponce JM, Oudit GY, Hall DD, Grueter CE
American journal of physiology. Heart and circulatory physiology312(4)H768-H780(2017 Apr)

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.
G?mez-Del Arco P, Perdiguero E, Yunes-Leites PS, Ac?n-P?rez R, Zeini M, Garcia-Gomez A, Sreenivasan K, Jim?nez-Alc?zar M, Segal?s J, L?pez-Maderuelo D, Orn?s B, Jim?nez-Borreguero LJ, D'Amato G, Enshell-Seijffers D, Morgan B, Georgopoulos K, Islam AB, Braun T, de la Pompa JL, Kim J, Enriquez JA, Ballestar E, Mu?oz-C?noves P, Redondo JM
Cell metabolism23(5)881-92(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The CS activity decreased in the infarcted tissue of wild-type (WT) mice at day 1 post-MI (p<0.05), but this was not observed in the MMP-9 null mice, suggesting that MMP-9 deletion helps to maintain the mitochondrial activity post-MI
Title: Citrate synthase is a novel in vivo matrix metalloproteinase-9 substrate that regulates mitochondrial function in the postmyocardial infarction left ventricle.

Multiple lines of evidence implicate this missense mutation (H55N) as the underlying cause of ahl4-related hearing loss, likely through its effects on mitochondrial adenosine trisphosphate (ATP) and free radical production in cochlear hair cells.
Title: Association of a citrate synthase missense mutation with age-related hearing loss in A/J mice.

H55N polymorphism in Cs could be the underlying cause of low CS activity and its high affinity for substrates in A/J mice compared with other strains.
Title: H55N polymorphism as a likely cause of variation in citrate synthase activity of mouse skeletal muscle.

A high-fat diet increases adiposity but maintains mitochondrial oxidative enzymes without affecting development of heart failure with pressure overload.
Title: A high-fat diet increases adiposity but maintains mitochondrial oxidative enzymes without affecting development of heart failure with pressure overload.

The following Cs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22138	NM_026444.4 Latest version!	Mus musculus citrate synthase (Cs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu85349	XM_030244861.1 Latest version!	Mus musculus citrate synthase (Cs), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu22138	NM_026444.3	Mus musculus citrate synthase (Cs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cs ( NM_026444.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080720.1 Mus musculus citrate synthase (Cs), mRNA.

Price & Availability↑

CloneID	OMu22138
Clone ID Related Accession (Same CDS sequence)	NM_026444.4 , NM_026444.3
Accession Version	NM_026444.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	citrate synthase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ144772.1, AC090489.8 and BU700437.1. On Jul 27, 2016 this sequence version replaced NM_026444.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK149990.1, SRR1660817.294434.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTAC TCACTGCAGC AACCCGGCTC TTGGGAGCCA AGAACTCATC CTGCCTCGTC 
CTTGCTGCCC GGCATGCCAG TGCTTCTTCC ACGAATTTGA AAGATGTACT GAGCAATCTG 
ATACCTAAGG AGCAGGCCAG AATTAAGACC TTCAAGCAGC AACATGGGAA GACAGTGGTG 
GGCCAGATCA CTGTGGACAT GATGTACGGT GGCATGAGAG GCATGAAGGG ACTTGTGTAT 
GAGACTTCGG TTCTTGATCC TGACGAGGGC ATCCGTTTCC GAGGCTACAG TATCCCTGAG 
TGCCAGAAAA TGCTGCCTAA GGCTAAGGGT GGAGAAGAAC CCCTGCCTGA GGGCTTATTT 
TGGCTGCTGG TAACTGGACA GATGCCCACA GAGGAACAGG TGTCTTGGCT CTCACGAGAA 
TGGGCAAAAA GGGCAGCTCT GCCTTCTCAT GTGGTCACCA TGCTGGACAA TTTTCCAACC 
AATCTGCACC CTATGTCTCA GCTCAGTGCA GCCATCACAG CCCTCAACAG TGAAAGCAAC 
TTCGCCCGGG CATATGCAGA GGGAATGAAC CGAGCTAAGT ACTGGGAGCT CATCTATGAG 
GACTGCATGG ACCTCATTGC CAAGCTGCCG TGCGTTGCAG CCAAGATCTA CCGGAATCTG 
TACCGGGAGG GCAGCAGTAT CGGAGCCATT GACTCTAGGC TAGACTGGTC ACACAATTTT 
ACCAACATGT TAGGCTACAC CGACCCTCAG TTCACCGAGC TCATGCGTTT GTACCTCACC 
ATCCATAGTG ACCATGAGGG TGGTAATGTA AGTGCCCACA CAAGCCATTT GGTGGGCAGC 
GCCCTTTCAG ACCCTTACCT GTCCTTTGCA GCAGCCATGA ATGGGCTGGC GGGGCCTCTA 
CATGGACTAG CAAATCAGGA GGTGCTTGTC TGGCTGACAC AGCTACAGAA GGAAGTTGGC 
AAAGACGTGT CAGATGAGAA GTTACGAGAC TACATCTGGA ACACACTCAA TTCAGGACGG 
GTGGTCCCAG GATACGGTCA TGCAGTACTG AGGAAGACTG ACCCTCGCTA TTCCTGTCAG 
CGAGAGTTTG CTCTGAAACA TCTGCCTAAG GATCCCATGT TCAAGCTGGT GGCTCAGCTG 
TACAAGATTG TGCCCAATAT CCTCTTAGAG CAAGGGAAGG CTAAGAACCC TTGGCCCAAC 
GTAGACGCTC ACAGTGGGGT GCTGCTCCAG TACTATGGCA TGACGGAGAT GAACTACTAC 
ACAGTCCTGT TTGGAGTGTC TCGGGCACTG GGTGTGCTAG CCCAGCTCAT CTGGAGCAGA 
GCCCTAGGCT TCCCCCTGGA AAGGCCCAAG TCCATGAGCA CGGATGGCCT GATGAAGTTT 
GTGGACTCTA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080720.1
CDS	192..1586
Translation	MALLTAATRLLGAKNSSCLVLAARHASASSTNLKDVLSNLIPKEQARIKTFKQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDPDEGIRFRGYSIPECQKMLPKAKGGEEPLPEGLFWLLVTGQMPTEEQVSWLSREWAKRAALPSHVVTMLDNFPTNLHPMSQLSAAITALNSESNFARAYAEGMNRAKYWELIYEDCMDLIAKLPCVAAKIYRNLYREGSSIGAIDSRLDWSHNFTNMLGYTDPQFTELMRLYLTIHSDHEGGNVSAHTSHLVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYSCQREFALKHLPKDPMFKLVAQLYKIVPNILLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTDGLMKFVDSK

Target ORF information:

RefSeq Version	NM_026444.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citrate synthase (Cs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026444.4

ORF Insert Sequence:

ATGGCTCTAC TCACTGCAGC AACCCGGCTC TTGGGAGCCA AGAACTCATC CTGCCTCGTC 
CTTGCTGCCC GGCATGCCAG TGCTTCTTCC ACGAATTTGA AAGATGTACT GAGCAATCTG 
ATACCTAAGG AGCAGGCCAG AATTAAGACC TTCAAGCAGC AACATGGGAA GACAGTGGTG 
GGCCAGATCA CTGTGGACAT GATGTACGGT GGCATGAGAG GCATGAAGGG ACTTGTGTAT 
GAGACTTCGG TTCTTGATCC TGACGAGGGC ATCCGTTTCC GAGGCTACAG TATCCCTGAG 
TGCCAGAAAA TGCTGCCTAA GGCTAAGGGT GGAGAAGAAC CCCTGCCTGA GGGCTTATTT 
TGGCTGCTGG TAACTGGACA GATGCCCACA GAGGAACAGG TGTCTTGGCT CTCACGAGAA 
TGGGCAAAAA GGGCAGCTCT GCCTTCTCAT GTGGTCACCA TGCTGGACAA TTTTCCAACC 
AATCTGCACC CTATGTCTCA GCTCAGTGCA GCCATCACAG CCCTCAACAG TGAAAGCAAC 
TTCGCCCGGG CATATGCAGA GGGAATGAAC CGAGCTAAGT ACTGGGAGCT CATCTATGAG 
GACTGCATGG ACCTCATTGC CAAGCTGCCG TGCGTTGCAG CCAAGATCTA CCGGAATCTG 
TACCGGGAGG GCAGCAGTAT CGGAGCCATT GACTCTAGGC TAGACTGGTC ACACAATTTT 
ACCAACATGT TAGGCTACAC CGACCCTCAG TTCACCGAGC TCATGCGTTT GTACCTCACC 
ATCCATAGTG ACCATGAGGG TGGTAATGTA AGTGCCCACA CAAGCCATTT GGTGGGCAGC 
GCCCTTTCAG ACCCTTACCT GTCCTTTGCA GCAGCCATGA ATGGGCTGGC GGGGCCTCTA 
CATGGACTAG CAAATCAGGA GGTGCTTGTC TGGCTGACAC AGCTACAGAA GGAAGTTGGC 
AAAGACGTGT CAGATGAGAA GTTACGAGAC TACATCTGGA ACACACTCAA TTCAGGACGG 
GTGGTCCCAG GATACGGTCA TGCAGTACTG AGGAAGACTG ACCCTCGCTA TTCCTGTCAG 
CGAGAGTTTG CTCTGAAACA TCTGCCTAAG GATCCCATGT TCAAGCTGGT GGCTCAGCTG 
TACAAGATTG TGCCCAATAT CCTCTTAGAG CAAGGGAAGG CTAAGAACCC TTGGCCCAAC 
GTAGACGCTC ACAGTGGGGT GCTGCTCCAG TACTATGGCA TGACGGAGAT GAACTACTAC 
ACAGTCCTGT TTGGAGTGTC TCGGGCACTG GGTGTGCTAG CCCAGCTCAT CTGGAGCAGA 
GCCCTAGGCT TCCCCCTGGA AAGGCCCAAG TCCATGAGCA CGGATGGCCT GATGAAGTTT 
GTGGACTCTA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cs ( NM_026444.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080720.1 Mus musculus citrate synthase (Cs), mRNA.

Price & Availability↑

CloneID	OMu22138
Clone ID Related Accession (Same CDS sequence)	NM_026444.4 , NM_026444.3
Accession Version	NM_026444.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-29
Organism	Mus musculus(House mouse)
Product	citrate synthase, mitochondrial precursor
Comment

ATGGCTCTAC TCACTGCAGC AACCCGGCTC TTGGGAGCCA AGAACTCATC CTGCCTCGTC 
CTTGCTGCCC GGCATGCCAG TGCTTCTTCC ACGAATTTGA AAGATGTACT GAGCAATCTG 
ATACCTAAGG AGCAGGCCAG AATTAAGACC TTCAAGCAGC AACATGGGAA GACAGTGGTG 
GGCCAGATCA CTGTGGACAT GATGTACGGT GGCATGAGAG GCATGAAGGG ACTTGTGTAT 
GAGACTTCGG TTCTTGATCC TGACGAGGGC ATCCGTTTCC GAGGCTACAG TATCCCTGAG 
TGCCAGAAAA TGCTGCCTAA GGCTAAGGGT GGAGAAGAAC CCCTGCCTGA GGGCTTATTT 
TGGCTGCTGG TAACTGGACA GATGCCCACA GAGGAACAGG TGTCTTGGCT CTCACGAGAA 
TGGGCAAAAA GGGCAGCTCT GCCTTCTCAT GTGGTCACCA TGCTGGACAA TTTTCCAACC 
AATCTGCACC CTATGTCTCA GCTCAGTGCA GCCATCACAG CCCTCAACAG TGAAAGCAAC 
TTCGCCCGGG CATATGCAGA GGGAATGAAC CGAGCTAAGT ACTGGGAGCT CATCTATGAG 
GACTGCATGG ACCTCATTGC CAAGCTGCCG TGCGTTGCAG CCAAGATCTA CCGGAATCTG 
TACCGGGAGG GCAGCAGTAT CGGAGCCATT GACTCTAGGC TAGACTGGTC ACACAATTTT 
ACCAACATGT TAGGCTACAC CGACCCTCAG TTCACCGAGC TCATGCGTTT GTACCTCACC 
ATCCATAGTG ACCATGAGGG TGGTAATGTA AGTGCCCACA CAAGCCATTT GGTGGGCAGC 
GCCCTTTCAG ACCCTTACCT GTCCTTTGCA GCAGCCATGA ATGGGCTGGC GGGGCCTCTA 
CATGGACTAG CAAATCAGGA GGTGCTTGTC TGGCTGACAC AGCTACAGAA GGAAGTTGGC 
AAAGACGTGT CAGATGAGAA GTTACGAGAC TACATCTGGA ACACACTCAA TTCAGGACGG 
GTGGTCCCAG GATACGGTCA TGCAGTACTG AGGAAGACTG ACCCTCGCTA TTCCTGTCAG 
CGAGAGTTTG CTCTGAAACA TCTGCCTAAG GATCCCATGT TCAAGCTGGT GGCTCAGCTG 
TACAAGATTG TGCCCAATAT CCTCTTAGAG CAAGGGAAGG CTAAGAACCC TTGGCCCAAC 
GTAGACGCTC ACAGTGGGGT GCTGCTCCAG TACTATGGCA TGACGGAGAT GAACTACTAC 
ACAGTCCTGT TTGGAGTGTC TCGGGCACTG GGTGTGCTAG CCCAGCTCAT CTGGAGCAGA 
GCCCTAGGCT TCCCCCTGGA AAGGCCCAAG TCCATGAGCA CGGATGGCCT GATGAAGTTT 
GTGGACTCTA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_080720.1

Translation

MALLTAATRLLGAKNSSCLVLAARHASASSTNLKDVLSNLIPKEQARIKTFKQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDPDEGIRFRGYSIPECQKMLPKAKGGEEPLPEGLFWLLVTGQMPTEEQVSWLSREWAKRAALPSHVVTMLDNFPTNLHPMSQLSAAITALNSESNFARAYAEGMNRAKYWELIYEDCMDLIAKLPCVAAKIYRNLYREGSSIGAIDSRLDWSHNFTNMLGYTDPQFTELMRLYLTIHSDHEGGNVSAHTSHLVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYSCQREFALKHLPKDPMFKLVAQLYKIVPNILLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTDGLMKFVDSK

Target ORF information:

RefSeq Version	NM_026444.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus citrate synthase (Cs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026444.3

ORF Insert Sequence:

ATGGCTCTAC TCACTGCAGC AACCCGGCTC TTGGGAGCCA AGAACTCATC CTGCCTCGTC 
CTTGCTGCCC GGCATGCCAG TGCTTCTTCC ACGAATTTGA AAGATGTACT GAGCAATCTG 
ATACCTAAGG AGCAGGCCAG AATTAAGACC TTCAAGCAGC AACATGGGAA GACAGTGGTG 
GGCCAGATCA CTGTGGACAT GATGTACGGT GGCATGAGAG GCATGAAGGG ACTTGTGTAT 
GAGACTTCGG TTCTTGATCC TGACGAGGGC ATCCGTTTCC GAGGCTACAG TATCCCTGAG 
TGCCAGAAAA TGCTGCCTAA GGCTAAGGGT GGAGAAGAAC CCCTGCCTGA GGGCTTATTT 
TGGCTGCTGG TAACTGGACA GATGCCCACA GAGGAACAGG TGTCTTGGCT CTCACGAGAA 
TGGGCAAAAA GGGCAGCTCT GCCTTCTCAT GTGGTCACCA TGCTGGACAA TTTTCCAACC 
AATCTGCACC CTATGTCTCA GCTCAGTGCA GCCATCACAG CCCTCAACAG TGAAAGCAAC 
TTCGCCCGGG CATATGCAGA GGGAATGAAC CGAGCTAAGT ACTGGGAGCT CATCTATGAG 
GACTGCATGG ACCTCATTGC CAAGCTGCCG TGCGTTGCAG CCAAGATCTA CCGGAATCTG 
TACCGGGAGG GCAGCAGTAT CGGAGCCATT GACTCTAGGC TAGACTGGTC ACACAATTTT 
ACCAACATGT TAGGCTACAC CGACCCTCAG TTCACCGAGC TCATGCGTTT GTACCTCACC 
ATCCATAGTG ACCATGAGGG TGGTAATGTA AGTGCCCACA CAAGCCATTT GGTGGGCAGC 
GCCCTTTCAG ACCCTTACCT GTCCTTTGCA GCAGCCATGA ATGGGCTGGC GGGGCCTCTA 
CATGGACTAG CAAATCAGGA GGTGCTTGTC TGGCTGACAC AGCTACAGAA GGAAGTTGGC 
AAAGACGTGT CAGATGAGAA GTTACGAGAC TACATCTGGA ACACACTCAA TTCAGGACGG 
GTGGTCCCAG GATACGGTCA TGCAGTACTG AGGAAGACTG ACCCTCGCTA TTCCTGTCAG 
CGAGAGTTTG CTCTGAAACA TCTGCCTAAG GATCCCATGT TCAAGCTGGT GGCTCAGCTG 
TACAAGATTG TGCCCAATAT CCTCTTAGAG CAAGGGAAGG CTAAGAACCC TTGGCCCAAC 
GTAGACGCTC ACAGTGGGGT GCTGCTCCAG TACTATGGCA TGACGGAGAT GAACTACTAC 
ACAGTCCTGT TTGGAGTGTC TCGGGCACTG GGTGTGCTAG CCCAGCTCAT CTGGAGCAGA 
GCCCTAGGCT TCCCCCTGGA AAGGCCCAAG TCCATGAGCA CGGATGGCCT GATGAAGTTT 
GTGGACTCTA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cs ( XM_030244861.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100721.1 Mus musculus citrate synthase (Cs), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu85349
Clone ID Related Accession (Same CDS sequence)	XM_030244861.1
Accession Version	XM_030244861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citrate synthase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTACG GTGGCATGAG AGGCATGAAG GGACTTGTGT ATGAGACTTC GGTTCTTGAT 
CCTGACGAGG GCATCCGTTT CCGAGGCTAC AGTATCCCTG AGTGCCAGAA AATGCTGCCT 
AAGGCTAAGG GTGGAGAAGA ACCCCTGCCT GAGGGCTTAT TTTGGCTGCT GGTAACTGGA 
CAGATGCCCA CAGAGGAACA GGTGTCTTGG CTCTCACGAG AATGGGCAAA AAGGGCAGCT 
CTGCCTTCTC ATGTGGTCAC CATGCTGGAC AATTTTCCAA CCAATCTGCA CCCTATGTCT 
CAGCTCAGTG CAGCCATCAC AGCCCTCAAC AGTGAAAGCA ACTTCGCCCG GGCATATGCA 
GAGGGAATGA ACCGAGCTAA GTACTGGGAG CTCATCTATG AGGACTGCAT GGACCTCATT 
GCCAAGCTGC CGTGCGTTGC AGCCAAGATC TACCGGAATC TGTACCGGGA GGGCAGCAGT 
ATCGGAGCCA TTGACTCTAG GCTAGACTGG TCACACAATT TTACCAACAT GTTAGGCTAC 
ACCGACCCTC AGTTCACCGA GCTCATGCGT TTGTACCTCA CCATCCATAG TGACCATGAG 
GGTGGTAATG TAAGTGCCCA CACAAGCCAT TTGGTGGGCA GCGCCCTTTC AGACCCTTAC 
CTGTCCTTTG CAGCAGCCAT GAATGGGCTG GCGGGGCCTC TACATGGACT AGCAAATCAG 
GAGGTGCTTG TCTGGCTGAC ACAGCTACAG AAGGAAGTTG GCAAAGACGT GTCAGATGAG 
AAGTTACGAG ACTACATCTG GAACACACTC AATTCAGGAC GGGTGGTCCC AGGATACGGT 
CATGCAGTAC TGAGGAAGAC TGACCCTCGC TATTCCTGTC AGCGAGAGTT TGCTCTGAAA 
CATCTGCCTA AGGATCCCAT GTTCAAGCTG GTGGCTCAGC TGTACAAGAT TGTGCCCAAT 
ATCCTCTTAG AGCAAGGGAA GGCTAAGAAC CCTTGGCCCA ACGTAGACGC TCACAGTGGG 
GTGCTGCTCC AGTACTATGG CATGACGGAG ATGAACTACT ACACAGTCCT GTTTGGAGTG 
TCTCGGGCAC TGGGTGTGCT AGCCCAGCTC ATCTGGAGCA GAGCCCTAGG CTTCCCCCTG 
GAAAGGCCCA AGTCCATGAG CACGGATGGC CTGATGAAGT TTGTGGACTC TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100721.1
CDS	200..1396
Translation	MMYGGMRGMKGLVYETSVLDPDEGIRFRGYSIPECQKMLPKAKGGEEPLPEGLFWLLVTGQMPTEEQVSWLSREWAKRAALPSHVVTMLDNFPTNLHPMSQLSAAITALNSESNFARAYAEGMNRAKYWELIYEDCMDLIAKLPCVAAKIYRNLYREGSSIGAIDSRLDWSHNFTNMLGYTDPQFTELMRLYLTIHSDHEGGNVSAHTSHLVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYSCQREFALKHLPKDPMFKLVAQLYKIVPNILLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTDGLMKFVDSK

Target ORF information:

RefSeq Version	XM_030244861.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citrate synthase (Cs), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244861.1

ORF Insert Sequence:

ATGATGTACG GTGGCATGAG AGGCATGAAG GGACTTGTGT ATGAGACTTC GGTTCTTGAT 
CCTGACGAGG GCATCCGTTT CCGAGGCTAC AGTATCCCTG AGTGCCAGAA AATGCTGCCT 
AAGGCTAAGG GTGGAGAAGA ACCCCTGCCT GAGGGCTTAT TTTGGCTGCT GGTAACTGGA 
CAGATGCCCA CAGAGGAACA GGTGTCTTGG CTCTCACGAG AATGGGCAAA AAGGGCAGCT 
CTGCCTTCTC ATGTGGTCAC CATGCTGGAC AATTTTCCAA CCAATCTGCA CCCTATGTCT 
CAGCTCAGTG CAGCCATCAC AGCCCTCAAC AGTGAAAGCA ACTTCGCCCG GGCATATGCA 
GAGGGAATGA ACCGAGCTAA GTACTGGGAG CTCATCTATG AGGACTGCAT GGACCTCATT 
GCCAAGCTGC CGTGCGTTGC AGCCAAGATC TACCGGAATC TGTACCGGGA GGGCAGCAGT 
ATCGGAGCCA TTGACTCTAG GCTAGACTGG TCACACAATT TTACCAACAT GTTAGGCTAC 
ACCGACCCTC AGTTCACCGA GCTCATGCGT TTGTACCTCA CCATCCATAG TGACCATGAG 
GGTGGTAATG TAAGTGCCCA CACAAGCCAT TTGGTGGGCA GCGCCCTTTC AGACCCTTAC 
CTGTCCTTTG CAGCAGCCAT GAATGGGCTG GCGGGGCCTC TACATGGACT AGCAAATCAG 
GAGGTGCTTG TCTGGCTGAC ACAGCTACAG AAGGAAGTTG GCAAAGACGT GTCAGATGAG 
AAGTTACGAG ACTACATCTG GAACACACTC AATTCAGGAC GGGTGGTCCC AGGATACGGT 
CATGCAGTAC TGAGGAAGAC TGACCCTCGC TATTCCTGTC AGCGAGAGTT TGCTCTGAAA 
CATCTGCCTA AGGATCCCAT GTTCAAGCTG GTGGCTCAGC TGTACAAGAT TGTGCCCAAT 
ATCCTCTTAG AGCAAGGGAA GGCTAAGAAC CCTTGGCCCA ACGTAGACGC TCACAGTGGG 
GTGCTGCTCC AGTACTATGG CATGACGGAG ATGAACTACT ACACAGTCCT GTTTGGAGTG 
TCTCGGGCAC TGGGTGTGCT AGCCCAGCTC ATCTGGAGCA GAGCCCTAGG CTTCCCCCTG 
GAAAGGCCCA AGTCCATGAG CACGGATGGC CTGATGAAGT TTGTGGACTC TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiac Med1 deletion promotes early lethality, cardiac remodeling, and transcriptional reprogramming.
American journal of physiology. Heart and circulatory physiology312(4)H768-H780(2017 Apr)
Spitler KM,Ponce JM,Oudit GY,Hall DD,Grueter CE

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Molecular and cellular biology36(15)2089-104(2016 Aug)
Bowman CE,Zhao L,Hartung T,Wolfgang MJ

The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.
Cell metabolism23(5)881-92(2016 May)
G?mez-Del Arco P,Perdiguero E,Yunes-Leites PS,Ac?n-P?rez R,Zeini M,Garcia-Gomez A,Sreenivasan K,Jim?nez-Alc?zar M,Segal?s J,L?pez-Maderuelo D,Orn?s B,Jim?nez-Borreguero LJ,D'Amato G,Enshell-Seijffers D,Morgan B,Georgopoulos K,Islam AB,Braun T,de la Pompa JL,Kim J,Enriquez JA,Ballestar E,Mu?oz-C?noves P,Redondo JM

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Cs cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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