Cryga cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cryga
Entrez Gene ID	12964
Full Name	crystallin, gamma A
Synonyms	Cryg-4,DGcry-4
General protein information	Preferred Names crystallin, gamma A Names gamma-crystallin A gamma-A-crystallin gamma-crystallin 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C2\|1 32.84 cM
Summary	Three main families of major soluble proteins, the alpha, beta and gamma crystallins, are ubiquitously expressed in vertebrate lenses. This gene encodes a member of the gamma-crystallin family of proteins which may function as a structural component of the eye lens. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Five gamma-crystallin genes (gamma-A through gamma-E) are tandemly organized in a genomic segment as a gene cluster in the mouse. Another gamma-crystallin gene (gamma-F) is found some distance upstream of the cluster on the same chromosome. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007774.3	NP_031800.1	gamma-crystallin A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	crygmx	NP_001013280.1
Pan troglodytes (chimpanzee)	CRYGA	NP_001094072.1
Canis lupus familiaris (dog)	CRYGA	NP_001106641.1
Mus musculus (house mouse)	Cryga	NP_031800.1
	CRYGA	NP_001094290.1
Bos taurus (cattle)	CRYGA	NP_001230499.1
Homo sapiens (human)	CRYGA	NP_055432.2
Rattus norvegicus (Norway rat)	Cryga	NP_001074405.1

Gene Ontology
Bibliography

Related articles in PubMed

A ?A-Crystallin Mouse Mutant Secc with Small Eye, Cataract and Closed Eyelid.
Cheng MH, Tam CN, Choy KW, Tsang WH, Tsang SL, Pang CP, Song YQ, Sham MH
PloS one11(8)e0160691(2016)

Disruption of Rest Leads to the Early Onset of Cataracts with the Aberrant Terminal Differentiation of Lens Fiber Cells.
Aoki H, Ogino H, Tomita H, Hara A, Kunisada T
PloS one11(9)e0163042(2016)

Expression of truncated PITX3 in the developing lens leads to microphthalmia and aphakia in mice.
Wada K, Matsushima Y, Tada T, Hasegawa S, Obara Y, Yoshizawa Y, Takahashi G, Hiai H, Shimanuki M, Suzuki S, Saitou J, Yamamoto N, Ichikawa M, Watanabe K, Kikkawa Y
PloS one9(10)e111432(2014)

The tumor suppressor, p53 regulates the ?A-crystallin gene during mouse lens development.
Hu XH, Nie Q, Yi M, Li TT, Wang ZF, Huang ZX, Gong XD, Zhou L, Ji WK, Hu WF, Liu JF, Wang L, Woodward Z, Zhu J, Liu WB, Nguyen QD, Li DW
Current molecular medicine14(9)1197-204(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

p53 regulates gammaA-crystallin gene expression during mouse lens development.
Title: The tumor suppressor, p53 regulates the ?A-crystallin gene during mouse lens development.

The following Cryga gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cryga cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00787	NM_007774.3 Latest version!	Mus musculus crystallin, gamma A (Cryga), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cryga ( NM_007774.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031800.1 Mus musculus crystallin, gamma A (Cryga), mRNA.

Price & Availability↑

CloneID	OMu00787
Clone ID Related Accession (Same CDS sequence)	NM_007774.3
Accession Version	NM_007774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	gamma-crystallin A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CF738355.1, BC056430.1 and CF728682.1. On Jan 13, 2004 this sequence version replaced NM_007774.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN539000.1, CF738355.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAAAGA TCACCTTCTA CGAGGACCGC GGCTTCCAGG GCCGCTGCTA TGAGTGCAGC 
AGCGACTGCC CCAACCTGCA GACCTACTTC AGCCGCTGCA ACTCCATCCG TGTGGACAGT 
GGCTGCTGGA TGCTCTATGA GCGCCCCAAC TACCAGGGCT ACCAGTACTT CCTGCGTCGC 
GGGGACTACC CTGACTACCA GCAGTGGATG GGTTTCAGCG ACTCCATCCG CTCCTGCCGT 
TCCATTCCAT ACACCAGCTC TCACAGGATA AGGCTTTATG AGAGAGATGA CTACCGGGGC 
CTTGTGTCTG AGCTCATGGA CGACTGTTCC TGCATCCACG ATCGCTTCCG CCTCCATGAG 
ATCTACTCCA TGCACGTGCT GGAGGGCTGC TGGGTCCTCT ATGAGATGCC CAACTACCGA 
GGCCGCCAAT ACCTGCTCAG GCCTGGAGAC TACAGGCGCT ACCACGACTG GGGCGCCATG 
GATGCCAAGG TCGGCTCTCT GAGACGGGTC ATGGATTTGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031800.1
CDS	27..551
Translation	MGKITFYEDRGFQGRCYECSSDCPNLQTYFSRCNSIRVDSGCWMLYERPNYQGYQYFLRRGDYPDYQQWMGFSDSIRSCRSIPYTSSHRIRLYERDDYRGLVSELMDDCSCIHDRFRLHEIYSMHVLEGCWVLYEMPNYRGRQYLLRPGDYRRYHDWGAMDAKVGSLRRVMDLY

Target ORF information:

RefSeq Version	NM_007774.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, gamma A (Cryga), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007774.3

ORF Insert Sequence:

ATGGGAAAGA TCACCTTCTA CGAGGACCGC GGCTTCCAGG GCCGCTGCTA TGAGTGCAGC 
AGCGACTGCC CCAACCTGCA GACCTACTTC AGCCGCTGCA ACTCCATCCG TGTGGACAGT 
GGCTGCTGGA TGCTCTATGA GCGCCCCAAC TACCAGGGCT ACCAGTACTT CCTGCGTCGC 
GGGGACTACC CTGACTACCA GCAGTGGATG GGTTTCAGCG ACTCCATCCG CTCCTGCCGT 
TCCATTCCAT ACACCAGCTC TCACAGGATA AGGCTTTATG AGAGAGATGA CTACCGGGGC 
CTTGTGTCTG AGCTCATGGA CGACTGTTCC TGCATCCACG ATCGCTTCCG CCTCCATGAG 
ATCTACTCCA TGCACGTGCT GGAGGGCTGC TGGGTCCTCT ATGAGATGCC CAACTACCGA 
GGCCGCCAAT ACCTGCTCAG GCCTGGAGAC TACAGGCGCT ACCACGACTG GGGCGCCATG 
GATGCCAAGG TCGGCTCTCT GAGACGGGTC ATGGATTTGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A ?A-Crystallin Mouse Mutant Secc with Small Eye, Cataract and Closed Eyelid.
PloS one11(8)e0160691(2016)
Cheng MH,Tam CN,Choy KW,Tsang WH,Tsang SL,Pang CP,Song YQ,Sham MH

Disruption of Rest Leads to the Early Onset of Cataracts with the Aberrant Terminal Differentiation of Lens Fiber Cells.
PloS one11(9)e0163042(2016)
Aoki H,Ogino H,Tomita H,Hara A,Kunisada T

Expression of truncated PITX3 in the developing lens leads to microphthalmia and aphakia in mice.
PloS one9(10)e111432(2014)
Wada K,Matsushima Y,Tada T,Hasegawa S,Obara Y,Yoshizawa Y,Takahashi G,Hiai H,Shimanuki M,Suzuki S,Saitou J,Yamamoto N,Ichikawa M,Watanabe K,Kikkawa Y

The tumor suppressor, p53 regulates the ?A-crystallin gene during mouse lens development.
Current molecular medicine14(9)1197-204(2014)
Hu XH,Nie Q,Yi M,Li TT,Wang ZF,Huang ZX,Gong XD,Zhou L,Ji WK,Hu WF,Liu JF,Wang L,Woodward Z,Zhu J,Liu WB,Nguyen QD,Li DW

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Cryga cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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