Cryba1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cryba1
Entrez Gene ID	12957
Full Name	crystallin, beta A1
Synonyms	BA3/A1,Cryb
General protein information	Preferred Names crystallin, beta A1 Names beta-crystallin A1 beta-A1-crystallin beta-A3/A1-crystallin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B5\|11 46.74 cM
Summary	Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009965.3	NP_034095.1	beta-crystallin A1 isoform 1
NM_001313933.1	NP_001300862.1	beta-crystallin A1 isoform 2

Pathways from BioSystems

Homologies

	CRYBA1	XP_001107784.1
Gallus gallus (chicken)	CRYBA1	NP_990833.2
Pan troglodytes (chimpanzee)	CRYBA1	XP_523590.2
Bos taurus (cattle)	CRYBA1	NP_776948.1
Mus musculus (house mouse)	Cryba1	NP_034095.1
Xenopus tropicalis (tropical clawed frog)	cryba1	NP_001096535.1
Homo sapiens (human)	CRYBA1	NP_005199.2
Rattus norvegicus (Norway rat)	Cryba1	NP_037188.1
Canis lupus familiaris (dog)	CRYBA1	NP_001074368.1
Danio rerio (zebrafish)	cryba1a	NP_001002410.1

Gene Ontology
Bibliography

Related articles in PubMed

Activating the AKT2-nuclear factor-?B-lipocalin-2 axis elicits an inflammatory response in age-related macular degeneration.
Ghosh S, Shang P, Yazdankhah M, Bhutto I, Hose S, Montezuma SR, Luo T, Chattopadhyay S, Qian J, Lutty GA, Ferrington DA, Zigler JS Jr, Sinha D
The Journal of pathology241(5)583-588(2017 Apr)

The amino acid transporter SLC36A4 regulates the amino acid pool in retinal pigmented epithelial cells and mediates the mechanistic target of rapamycin, complex 1 signaling.
Shang P, Valapala M, Grebe R, Hose S, Ghosh S, Bhutto IA, Handa JT, Lutty GA, Lu L, Wan J, Qian J, Sergeev Y, Puertollano R, Zigler JS Jr, Xu GT, Sinha D
Aging cell16(2)349-359(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest a mechanism by which betaA3/A1-crystallin regulates lysosomal function by modulating the activity of V-ATPase.
Title: Modulation of V-ATPase by ?A3/A1-Crystallin in Retinal Pigment Epithelial Cells.

CRYbetaA3/A1-crystallin has a role in preventing nuclear cataract impaired lysosomal cargo clearance and calpain activation
Title: CRY?A3/A1-Crystallin Knockout Develops Nuclear Cataract and Causes Impaired Lysosomal Cargo Clearance and Calpain Activation.

Data show that betaA3/A1-crystallin affects the signal transducer and activator of transcription 3 (STAT3) activation in optic nerve astrocytes.
Title: ?A3/A1-crystallin is a critical mediator of STAT3 signaling in optic nerve astrocytes.

loss of CRYBA1 causes lysosomal dysregulation leading to the impairment of both autophagy and phagocytosis
Title: Lysosomal-mediated waste clearance in retinal pigment epithelial cells is regulated by CRYBA1/?A3/A1-crystallin via V-ATPase-MTORC1 signaling.

p53 can regulate lens differentiation by controlling expression of the differentiation genes coding for the lens crystallins.
Title: p53 directly regulates ?A- and ?A3/A1-crystallin genes to modulate lens differentiation.

The following Cryba1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cryba1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13006	NM_009965.3 Latest version!	Mus musculus crystallin, beta A1 (Cryba1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu45510	NM_001313933.1 Latest version!	Mus musculus crystallin, beta A1 (Cryba1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cryba1 ( NM_009965.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034095.1 Mus musculus crystallin, beta A1 (Cryba1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13006
Clone ID Related Accession (Same CDS sequence)	NM_009965.3
Accession Version	NM_009965.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	beta-crystallin A1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ239052.1 and CN539082.1. On Sep 14, 2015 this sequence version replaced NM_009965.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN539082.1, CB848756.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACCC AGACTGTGCA GCGGGAGTTG GAAACTCTTC CAACCACCAA GATGGCTCAG 
ACCAACCCTA TGCCAGGATC CTTGGGGCCA TGGAAGATAA CCATCTACGA TCAAGAGAAC 
TTCCAGGGCA AGAGGATGGA GTTCACCAGC TCCTGCCCAA ATGTCTCTGA ACGTAATTTT 
GATAATGTCC GGTCACTTAA GGTGGAGTGT GGCGCCTGGA TTGGTTATGA ACACACCAGC 
TTCTGTGGGC AACAGTTCAT CCTGGAAAGA GGAGAATACC CTCGATGGGA TGCCTGGAGC 
GGGAGCAATG CCTATCATAT TGAGCGTCTC ATGTCCTTCC GACCCATCTG TTCCGCTAAT 
CATAAAGAGT CTAAGATTAC CATCTTCGAG AAAGAGAACT TTATTGGACG CCAGTGGGAA 
ATCTGTGATG ACTACCCTTC CTTGCAAGCC ATGGGTTGGT TCAACAATGA AGTTGGTTCC 
ATGAAGATAC AATGTGGGGC CTGGGTTTGC TACCAGTACC CTGGATATCG TGGTTATCAG 
TATATCTTGG AGTGTGACCA CCATGGAGGA GACTACAAGC ACTGGCCAGA GTGGGGATCT 
CACGCCCAGA CTTCCCAGAT CCAATCAATT CGCCGAATAC AACAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034095.1
CDS	13..660
Translation	METQTVQRELETLPTTKMAQTNPMPGSLGPWKITIYDQENFQGKRMEFTSSCPNVSERNFDNVRSLKVECGAWIGYEHTSFCGQQFILERGEYPRWDAWSGSNAYHIERLMSFRPICSANHKESKITIFEKENFIGRQWEICDDYPSLQAMGWFNNEVGSMKIQCGAWVCYQYPGYRGYQYILECDHHGGDYKHWPEWGSHAQTSQIQSIRRIQQ

Target ORF information:

RefSeq Version	NM_009965.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, beta A1 (Cryba1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009965.3

ORF Insert Sequence:

ATGGAGACCC AGACTGTGCA GCGGGAGTTG GAAACTCTTC CAACCACCAA GATGGCTCAG 
ACCAACCCTA TGCCAGGATC CTTGGGGCCA TGGAAGATAA CCATCTACGA TCAAGAGAAC 
TTCCAGGGCA AGAGGATGGA GTTCACCAGC TCCTGCCCAA ATGTCTCTGA ACGTAATTTT 
GATAATGTCC GGTCACTTAA GGTGGAGTGT GGCGCCTGGA TTGGTTATGA ACACACCAGC 
TTCTGTGGGC AACAGTTCAT CCTGGAAAGA GGAGAATACC CTCGATGGGA TGCCTGGAGC 
GGGAGCAATG CCTATCATAT TGAGCGTCTC ATGTCCTTCC GACCCATCTG TTCCGCTAAT 
CATAAAGAGT CTAAGATTAC CATCTTCGAG AAAGAGAACT TTATTGGACG CCAGTGGGAA 
ATCTGTGATG ACTACCCTTC CTTGCAAGCC ATGGGTTGGT TCAACAATGA AGTTGGTTCC 
ATGAAGATAC AATGTGGGGC CTGGGTTTGC TACCAGTACC CTGGATATCG TGGTTATCAG 
TATATCTTGG AGTGTGACCA CCATGGAGGA GACTACAAGC ACTGGCCAGA GTGGGGATCT 
CACGCCCAGA CTTCCCAGAT CCAATCAATT CGCCGAATAC AACAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cryba1 ( NM_001313933.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300862.1 Mus musculus crystallin, beta A1 (Cryba1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu45510
Clone ID Related Accession (Same CDS sequence)	NM_001313933.1
Accession Version	NM_001313933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	beta-crystallin A1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ239052.1, CK628527.1 and CN539082.1. Transcript Variant: This variant (2) lacks an alternate internal exon compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CX768313.1, CX768681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164141, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACCC AGACTGTGCA GCGGGAGTTG GAAACTCTTC CAACCACCAA GATGGCTCAG 
ACCAACCCTA TGCCAGGATC CTTGGGGCCA TGGAAGATAA CCATCTACGA TCAAGAGAAC 
TTCCAGGGCA AGAGGATGGA GTTCACCAGC TCCTGCCCAA ATGTCTCTGA ACGTAATTTT 
GATAATGTCC GGTCACTTAA GGTGGAGTGT GGCGCAATCA TAAAGAGTCT AAGATTACCA 
TCTTCGAGAA AGAGAACTTT ATTGGACGCC AGTGGGAAAT CTGTGATGAC TACCCTTCCT 
TGCAAGCCAT GGGTTGGTTC AACAATGAAG TTGGTTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300862.1
CDS	13..354
Translation	METQTVQRELETLPTTKMAQTNPMPGSLGPWKITIYDQENFQGKRMEFTSSCPNVSERNFDNVRSLKVECGAIIKSLRLPSSRKRTLLDASGKSVMTTLPCKPWVGSTMKLVP

Target ORF information:

RefSeq Version	NM_001313933.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, beta A1 (Cryba1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313933.1

ORF Insert Sequence:

ATGGAGACCC AGACTGTGCA GCGGGAGTTG GAAACTCTTC CAACCACCAA GATGGCTCAG 
ACCAACCCTA TGCCAGGATC CTTGGGGCCA TGGAAGATAA CCATCTACGA TCAAGAGAAC 
TTCCAGGGCA AGAGGATGGA GTTCACCAGC TCCTGCCCAA ATGTCTCTGA ACGTAATTTT 
GATAATGTCC GGTCACTTAA GGTGGAGTGT GGCGCAATCA TAAAGAGTCT AAGATTACCA 
TCTTCGAGAA AGAGAACTTT ATTGGACGCC AGTGGGAAAT CTGTGATGAC TACCCTTCCT 
TGCAAGCCAT GGGTTGGTTC AACAATGAAG TTGGTTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activating the AKT2-nuclear factor-?B-lipocalin-2 axis elicits an inflammatory response in age-related macular degeneration.
The Journal of pathology241(5)583-588(2017 Apr)
Ghosh S,Shang P,Yazdankhah M,Bhutto I,Hose S,Montezuma SR,Luo T,Chattopadhyay S,Qian J,Lutty GA,Ferrington DA,Zigler JS Jr,Sinha D

The amino acid transporter SLC36A4 regulates the amino acid pool in retinal pigmented epithelial cells and mediates the mechanistic target of rapamycin, complex 1 signaling.
Aging cell16(2)349-359(2017 Apr)
Shang P,Valapala M,Grebe R,Hose S,Ghosh S,Bhutto IA,Handa JT,Lutty GA,Lu L,Wan J,Qian J,Sergeev Y,Puertollano R,Zigler JS Jr,Xu GT,Sinha D

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Cryba1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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