Cryaa cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cryaa
Entrez Gene ID	12954
Full Name	crystallin, alpha A
Synonyms	Acry-1,Crya-1,Crya1,DAcry-1,lop18
General protein information	Preferred Names crystallin, alpha A Names alpha-crystallin A chain crystallin, alpha 1 lens opacity 18
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 17.09 cM
Summary	This gene encodes subunit a, one of two subunits of alpha-crystallin, which is a high molecular weight, soluble aggregate and is a member of the small heat shock protein (sHSP) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. It acts as a molecular chaperone and is the major protein in the eye lens, maintaining the transparency and refractive index of the lens. In mouse, deficiency in this gene is associated with smaller lenses and eyes and with increasing lens opacity with age. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001278569.1	NP_001265498.1	alpha-crystallin A chain isoform 1
NM_013501.3	NP_038529.1	alpha-crystallin A chain isoform 2
NM_001278570.1	NP_001265499.1	alpha-crystallin A chain isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CRYAA	NP_000385.1
Pan troglodytes (chimpanzee)	CRYAA	XP_514927.2
Canis lupus familiaris (dog)	CRYAA	NP_001074367.1
Mus musculus (house mouse)	Cryaa	NP_038529.1
Gallus gallus (chicken)	CRYAA	NP_001025968.1
Homo sapiens (human)	LOC102724652	XP_006723993.1
Danio rerio (zebrafish)	cryaa	NP_694482.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007159	XP_308609.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005548	XP_315550.4
Bos taurus (cattle)	CRYAA	NP_776714.1
Rattus norvegicus (Norway rat)	Cryaa	NP_036666.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007158	XP_308610.4
Xenopus tropicalis (tropical clawed frog)	LOC100488185	XP_002944595.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005547	XP_315549.4

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a missense mutation in the alphaA-crystallin gene of the lop18 mouse.
Chang B, Hawes NL, Roderick TH, Smith RS, Heckenlively JR, Horwitz J, Davisson MT
Molecular vision521(1999 Sep)

Gene sharing in lens and cornea: facts and implications.
Piatigorsky J
Progress in retinal and eye research17(2)145-74(1998 Apr)

Targeted disruption of the mouse alpha A-crystallin gene induces cataract and cytoplasmic inclusion bodies containing the small heat shock protein alpha B-crystallin.
Brady JP, Garland D, Duglas-Tabor Y, Robison WG Jr, Groome A, Wawrousek EF
Proceedings of the National Academy of Sciences of the United States of America94(3)884-9(1997 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Collectively, these studies show that FGF signaling up-regulates expression of alphaA-crystallin both directly and indirectly via up-regulation of c-Maf.
Title: Regulation of c-Maf and ?A-Crystallin in Ocular Lens by Fibroblast Growth Factor Signaling.

Knockout of alphaA-crystallin inhibited pathologic neovascularization through the VEGF and VEGFR2 signaling pathways both in vitro and in vivo.
Title: Knockout of ?A-crystallin inhibits ocular neovascularization.

the expression of alphaAN101D transgene results in disruption of lens morphogenesis, which is characterized by defective LEC proliferation, elongation, and lens fiber cell denucleation.
Title: Molecular mechanism of formation of cortical opacity in CRYAAN101D transgenic mice.

p53 can regulate lens differentiation by controlling expression of the differentiation genes coding for the lens crystallins.
Title: p53 directly regulates ?A- and ?A3/A1-crystallin genes to modulate lens differentiation.

alphaA-crystallin may protect against geographic atrophy.
Title: ?A crystallin may protect against geographic atrophy-meta-analysis of cataract vs. cataract surgery for geographic atrophy and experimental studies.

The following Cryaa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cryaa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23185	NM_001278570.1 Latest version!	Mus musculus crystallin, alpha A (Cryaa), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu13663	NM_013501.3 Latest version!	Mus musculus crystallin, alpha A (Cryaa), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu13772	NM_001278569.1 Latest version!	Mus musculus crystallin, alpha A (Cryaa), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cryaa ( NM_001278570.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001265499.1 Mus musculus crystallin, alpha A (Cryaa), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu23185
Clone ID Related Accession (Same CDS sequence)	NM_001278570.1
Accession Version	NM_001278570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Mus musculus(house mouse)
Product	alpha-crystallin A chain isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EL604937.1, AK143463.1 and BB371615.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 3 which is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB844022.2, AK143463.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 9695791 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACGTCA CCATTCAGCA TCCTTGGTTC AAGCGTGCCC TGGGGCCCTT CTACCCCAGC 
CGACTGTTCG ACCAGTTCTT CGGCGAGGGC CTTTTTGAGT ACGACCTGCT GCCCTTCCTG 
TCTTCCACCA TCAGCCCCTA CTACCGCCAG TCCCTCTTCC GCACTGTGCT GGACTCGGGC 
ATCTCTGAGG TCCGATCTGA CCGGGACAAG TTTGTCATCT TCTTGGACGT GAAGCACTTC 
TCTCCTGAGG ACCTCACCGT GAAGGTACTG GAGGATTTTG TGGAGATTCA CGGCAAACAC 
AACGAGAGGC AGGATGACCA TGGCTACATT TCCCGTGAAT TTCACCGTCG CTACCGTCTG 
CCTTCCAATG TGGACCAGTC CGCCCTCTCC TGCTCCCTGT CTGCGGATGG CATGCTGACC 
TTCTCTGGCC CCAAGGTCCA GTCCGGTTTG GATGCTGGCC ACAGCGAGAG GGCCATTCCT 
GTGTCACGGG AGGAGAAACC CAGCTCTGCA CCCTCGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265499.1
CDS	71..592
Translation	MDVTIQHPWFKRALGPFYPSRLFDQFFGEGLFEYDLLPFLSSTISPYYRQSLFRTVLDSGISEVRSDRDKFVIFLDVKHFSPEDLTVKVLEDFVEIHGKHNERQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFSGPKVQSGLDAGHSERAIPVSREEKPSSAPSS

Target ORF information:

RefSeq Version	NM_001278570.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, alpha A (Cryaa), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278570.1

ORF Insert Sequence:

ATGGACGTCA CCATTCAGCA TCCTTGGTTC AAGCGTGCCC TGGGGCCCTT CTACCCCAGC 
CGACTGTTCG ACCAGTTCTT CGGCGAGGGC CTTTTTGAGT ACGACCTGCT GCCCTTCCTG 
TCTTCCACCA TCAGCCCCTA CTACCGCCAG TCCCTCTTCC GCACTGTGCT GGACTCGGGC 
ATCTCTGAGG TCCGATCTGA CCGGGACAAG TTTGTCATCT TCTTGGACGT GAAGCACTTC 
TCTCCTGAGG ACCTCACCGT GAAGGTACTG GAGGATTTTG TGGAGATTCA CGGCAAACAC 
AACGAGAGGC AGGATGACCA TGGCTACATT TCCCGTGAAT TTCACCGTCG CTACCGTCTG 
CCTTCCAATG TGGACCAGTC CGCCCTCTCC TGCTCCCTGT CTGCGGATGG CATGCTGACC 
TTCTCTGGCC CCAAGGTCCA GTCCGGTTTG GATGCTGGCC ACAGCGAGAG GGCCATTCCT 
GTGTCACGGG AGGAGAAACC CAGCTCTGCA CCCTCGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cryaa ( NM_013501.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038529.1 Mus musculus crystallin, alpha A (Cryaa), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13663
Clone ID Related Accession (Same CDS sequence)	NM_013501.3
Accession Version	NM_013501.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Mus musculus(house mouse)
Product	alpha-crystallin A chain isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EL605017.1, BC085170.1 and BB371615.1. On Jun 26, 2013 this sequence version replaced NM_013501.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region compared to variant 1. It encodes isoform 2, also known as alpha-A-ins, which is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085170.1, CV561151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 9695791 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACGTCA CCATTCAGCA TCCTTGGTTC AAGCGTGCCC TGGGGCCCTT CTACCCCAGC 
CGACTGTTCG ACCAGTTCTT CGGCGAGGGC CTTTTTGAGT ACGACCTGCT GCCCTTCCTG 
TCTTCCACCA TCAGCCCCTA CTACCGCCAG TCCCTCTTCC GCACTGTGCT GGACTCGGGC 
ATCTCTGAGC TCATGACCCA TATGTGGTTT GTAATGCACC AACCACATGC TGGAAACCCC 
AAGAACAACC CCGTCAAGGT CCGATCTGAC CGGGACAAGT TTGTCATCTT CTTGGACGTG 
AAGCACTTCT CTCCTGAGGA CCTCACCGTG AAGGTACTGG AGGATTTTGT GGAGATTCAC 
GGCAAACACA ACGAGAGGCA GGATGACCAT GGCTACATTT CCCGTGAATT TCACCGTCGC 
TACCGTCTGC CTTCCAATGT GGACCAGTCC GCCCTCTCCT GCTCCCTGTC TGCGGATGGC 
ATGCTGACCT TCTCTGGCCC CAAGGTCCAG TCCGGTTTGG ATGCTGGCCA CAGCGAGAGG 
GCCATTCCTG TGTCACGGGA GGAGAAACCC AGCTCTGCAC CCTCGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038529.1
CDS	71..661
Translation	MDVTIQHPWFKRALGPFYPSRLFDQFFGEGLFEYDLLPFLSSTISPYYRQSLFRTVLDSGISELMTHMWFVMHQPHAGNPKNNPVKVRSDRDKFVIFLDVKHFSPEDLTVKVLEDFVEIHGKHNERQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFSGPKVQSGLDAGHSERAIPVSREEKPSSAPSS

Target ORF information:

RefSeq Version	NM_013501.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, alpha A (Cryaa), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013501.3

ORF Insert Sequence:

ATGGACGTCA CCATTCAGCA TCCTTGGTTC AAGCGTGCCC TGGGGCCCTT CTACCCCAGC 
CGACTGTTCG ACCAGTTCTT CGGCGAGGGC CTTTTTGAGT ACGACCTGCT GCCCTTCCTG 
TCTTCCACCA TCAGCCCCTA CTACCGCCAG TCCCTCTTCC GCACTGTGCT GGACTCGGGC 
ATCTCTGAGC TCATGACCCA TATGTGGTTT GTAATGCACC AACCACATGC TGGAAACCCC 
AAGAACAACC CCGTCAAGGT CCGATCTGAC CGGGACAAGT TTGTCATCTT CTTGGACGTG 
AAGCACTTCT CTCCTGAGGA CCTCACCGTG AAGGTACTGG AGGATTTTGT GGAGATTCAC 
GGCAAACACA ACGAGAGGCA GGATGACCAT GGCTACATTT CCCGTGAATT TCACCGTCGC 
TACCGTCTGC CTTCCAATGT GGACCAGTCC GCCCTCTCCT GCTCCCTGTC TGCGGATGGC 
ATGCTGACCT TCTCTGGCCC CAAGGTCCAG TCCGGTTTGG ATGCTGGCCA CAGCGAGAGG 
GCCATTCCTG TGTCACGGGA GGAGAAACCC AGCTCTGCAC CCTCGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cryaa ( NM_001278569.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001265498.1 Mus musculus crystallin, alpha A (Cryaa), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13772
Clone ID Related Accession (Same CDS sequence)	NM_001278569.1
Accession Version	NM_001278569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Mus musculus(house mouse)
Product	alpha-crystallin A chain isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EL605017.1, BY114969.1, CX734348.1, CA452236.1 and BB371615.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EL962835.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849380, SAMN00849383 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 9695791 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACGTCA CCATTCAGCA TCCTTGGTTC AAGCGTGCCC TGGGGCCCTT CTACCCCAGC 
CGACTGTTCG ACCAGTTCTT CGGCGAGGGC CTTTTTGAGT ACGACCTGCT GCCCTTCCTG 
TCTTCCACCA TCAGCCCCTA CTACCGCCAG TCCCTCTTCC GCACTGTGCT GGACTCGGGC 
ATCTCTGAGC TCATGACCCA TATGTGGTTT GTAATGCACC AACCACATGC TGGAAACCCC 
AAGAACAACC CCGTCAAGGC AAGTTACATG GCGAAGGTCC GATCTGACCG GGACAAGTTT 
GTCATCTTCT TGGACGTGAA GCACTTCTCT CCTGAGGACC TCACCGTGAA GGTACTGGAG 
GATTTTGTGG AGATTCACGG CAAACACAAC GAGAGGCAGG ATGACCATGG CTACATTTCC 
CGTGAATTTC ACCGTCGCTA CCGTCTGCCT TCCAATGTGG ACCAGTCCGC CCTCTCCTGC 
TCCCTGTCTG CGGATGGCAT GCTGACCTTC TCTGGCCCCA AGGTCCAGTC CGGTTTGGAT 
GCTGGCCACA GCGAGAGGGC CATTCCTGTG TCACGGGAGG AGAAACCCAG CTCTGCACCC 
TCGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265498.1
CDS	71..679
Translation	MDVTIQHPWFKRALGPFYPSRLFDQFFGEGLFEYDLLPFLSSTISPYYRQSLFRTVLDSGISELMTHMWFVMHQPHAGNPKNNPVKASYMAKVRSDRDKFVIFLDVKHFSPEDLTVKVLEDFVEIHGKHNERQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFSGPKVQSGLDAGHSERAIPVSREEKPSSAPSS

Target ORF information:

RefSeq Version	NM_001278569.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, alpha A (Cryaa), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278569.1

ORF Insert Sequence:

ATGGACGTCA CCATTCAGCA TCCTTGGTTC AAGCGTGCCC TGGGGCCCTT CTACCCCAGC 
CGACTGTTCG ACCAGTTCTT CGGCGAGGGC CTTTTTGAGT ACGACCTGCT GCCCTTCCTG 
TCTTCCACCA TCAGCCCCTA CTACCGCCAG TCCCTCTTCC GCACTGTGCT GGACTCGGGC 
ATCTCTGAGC TCATGACCCA TATGTGGTTT GTAATGCACC AACCACATGC TGGAAACCCC 
AAGAACAACC CCGTCAAGGC AAGTTACATG GCGAAGGTCC GATCTGACCG GGACAAGTTT 
GTCATCTTCT TGGACGTGAA GCACTTCTCT CCTGAGGACC TCACCGTGAA GGTACTGGAG 
GATTTTGTGG AGATTCACGG CAAACACAAC GAGAGGCAGG ATGACCATGG CTACATTTCC 
CGTGAATTTC ACCGTCGCTA CCGTCTGCCT TCCAATGTGG ACCAGTCCGC CCTCTCCTGC 
TCCCTGTCTG CGGATGGCAT GCTGACCTTC TCTGGCCCCA AGGTCCAGTC CGGTTTGGAT 
GCTGGCCACA GCGAGAGGGC CATTCCTGTG TCACGGGAGG AGAAACCCAG CTCTGCACCC 
TCGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of a missense mutation in the alphaA-crystallin gene of the lop18 mouse.
Molecular vision521(1999 Sep)
Chang B,Hawes NL,Roderick TH,Smith RS,Heckenlively JR,Horwitz J,Davisson MT

Gene sharing in lens and cornea: facts and implications.
Progress in retinal and eye research17(2)145-74(1998 Apr)
Piatigorsky J

Targeted disruption of the mouse alpha A-crystallin gene induces cataract and cytoplasmic inclusion bodies containing the small heat shock protein alpha B-crystallin.
Proceedings of the National Academy of Sciences of the United States of America94(3)884-9(1997 Feb)
Brady JP,Garland D,Duglas-Tabor Y,Robison WG Jr,Groome A,Wawrousek EF

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Cryaa cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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