Cpe cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpe
Entrez Gene ID	12876
Full Name	carboxypeptidase E
Synonyms	CPH,Cph-1,Cph1,NF-alpha1,R74677,fat
General protein information	Preferred Names carboxypeptidase E Names carboxypeptidase E carboxypeptidase H enkephalin convertase prohormone-processing carboxypeptidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 B3.1\|8 32.3 cM
Summary	This gene encodes carboxypeptidase E, a prohormone-processing exopeptidase found in secretory granules of endocrine and neuroendocrine cells. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that cleaves the C-terminal basic residues of protein substrates. A missense mutation in this gene is responsible for the obesity phenotype in a mouse model known as the 'fat mouse.' Mice lacking the functional product of this gene exhibit impaired processing of multiple peptide hormones such as proinsulin, prodynorphin, proneurotensin, promelanin-concentrating hormone and pro-opiomelanocortin. [provided by RefSeq, Jan 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013494.4	NP_038522.2	carboxypeptidase E preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cpe	NP_037260.1
Xenopus tropicalis (tropical clawed frog)	cpe	NP_001106417.1
	CPE	XP_001097378.2
Danio rerio (zebrafish)	cpe	NP_999975.1
Pan troglodytes (chimpanzee)	CPE	NP_001092029.1
Caenorhabditis elegans (roundworm)	egl-21	NP_501935.1
Homo sapiens (human)	CPE	NP_001864.1
Bos taurus (cattle)	CPE	NP_776328.2
Mus musculus (house mouse)	Cpe	NP_038522.2
Canis lupus familiaris (dog)	CPE	XP_005629450.1
Gallus gallus (chicken)	CPE	XP_420392.3

Gene Ontology
Bibliography

Related articles in PubMed

Augmented Responses to Ozone in Obese Mice Require IL-17A and Gastrin-releasing Peptide.
Mathews JA, Krishnamoorthy N, Kasahara DI, Hutchinson J, Cho Y, Brand JD, Williams AS, Wurmbrand AP, Ribeiro L, Cuttitta F, Sunday ME, Levy BD, Shore SA
American journal of respiratory cell and molecular biology(2017 Sep)

Absence of Carboxypeptidase E/Neurotrophic Factor-?1 in Knock-Out Mice Leads to Dysfunction of BDNF-TRKB Signaling in Hippocampus.
Xiao L, Chang SY, Xiong ZG, Selveraj P, Peng Loh Y
Journal of molecular neuroscience : MN62(1)79-87(2017 May)

Neurotrophic Factor-?1: A Key Wnt-?-Catenin Dependent Anti-Proliferation Factor and ERK-Sox9 Activated Inducer of Embryonic Neural Stem Cell Differentiation to Astrocytes in Neurodevelopment.
Selvaraj P, Xiao L, Lee C, Murthy SR, Cawley NX, Lane M, Merchenthaler I, Ahn S, Loh YP
Stem cells (Dayton, Ohio)35(3)557-571(2017 Mar)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NF-alpha1 is critical for regulating antiproliferation and cell fate determination, through differentiating embryonic stem cells to GFAP-positive astrocytes for normal neurodevelopment.
Title: Neurotrophic Factor-?1: A Key Wnt-?-Catenin Dependent Anti-Proliferation Factor and ERK-Sox9 Activated Inducer of Embryonic Neural Stem Cell Differentiation to Astrocytes in Neurodevelopment.

we show by RNA silencing that CPE and SgIII play a synergistic role in the trafficking of POMC to granules of the regulated secretory pathway in AtT20 cells
Title: Carboxypeptidase E and Secretogranin III Coordinately Facilitate Efficient Sorting of Proopiomelanocortin to the Regulated Secretory Pathway in AtT20 Cells.

This study shown that NF-alpha1-mediated upregulation of hippocampal FGF2 and neurogenesis prevents an onset of chronic stress-induced depressive-like behavior
Title: Neurotrophic factor-?1 prevents stress-induced depression through enhancement of neurogenesis and is activated by rosiglitazone.

Carboxypeptidase E is an important modulator of RANKL-induced osteoclast differentiation.
Title: Carboxypeptidase E is a novel modulator of RANKL-induced osteoclast differentiation.

The neuroprotective effect of CPE-DeltaN against neuronal glutamate neurotoxicity was mediated by secreted FGF2.
Title: carboxypeptidase E-?N, a neuroprotein transiently expressed during development protects embryonic neurons against glutamate neurotoxicity.

The following Cpe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17754	NM_013494.4 Latest version!	Mus musculus carboxypeptidase E (Cpe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$279.30 $399.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpe ( NM_013494.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038522.2 Mus musculus carboxypeptidase E (Cpe), mRNA.

Price & Availability↑

CloneID	OMu17754
Clone ID Related Accession (Same CDS sequence)	NM_013494.4
Accession Version	NM_013494.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	carboxypeptidase E preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CX241703.1, AK047891.1 and BE849320.1. On Jan 26, 2016 this sequence version replaced NM_013494.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010197.1, SRR1660811.112356.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGGC GCGGAGGACG GGTGCTGCTG GCGCTGTGTG CCGCGCTGGT GGCCGGCGGG 
TGGCTGCTGA CGGCTGAAGC CCAGGAGCCC GGGGCGCCAG CGGCTGGCAT GAGGCGCCGC 
CGGCGGCTCC AGCAAGAGGA CGGCATCTCC TTCGAGTACC ACCGCTATCC AGAGCTGCGC 
GAGGCGCTGG TGTCCGTATG GCTGCAGTGC ACCGCCATCA GCAGAATCTA CACAGTGGGG 
CGCAGCTTCG AGGGCCGGGA GCTCCTGGTC ATCGAGCTGT CTGACAACCC CGGGGTCCAT 
GAGCCGGGTG AACCTGAATT TAAATACATT GGGAACATGC ATGGTAATGA GGCGGTTGGA 
CGGGAACTGC TTATTTTCTT GGCCCAGTAC CTGTGTAACG AGTACCAGAA AGGCAATGAG 
ACAATTGTCA ACCTGATCCA CAGCACCCGA ATTCATATCA TGCCCTCCTT GAACCCCGAC 
GGCTTTGAGA AAGCCGCATC GCAGCCCGGC GAGCTGAAGG ACTGGTTTGT GGGCCGCAGC 
AACGCCCAGG GAATAGATCT GAACCGTAAC TTCCCAGACC TGGACAGGAT CGTGTATGTT 
AATGAGAAAG AAGGCGGTCC TAACAATCAC CTGCTGAAGA ATCTGAAGAA AATTGTGGAC 
CAAAATTCAA AGCTTGCCCC CGAGACCAAG GCTGTCATTC ACTGGATCAT GGACATTCCA 
TTTGTGCTTT CTGCCAATCT GCACGGAGGA GACCTTGTGG CTAATTACCC ATATGATGAG 
ACACGGAGCG GTACTGCTCA CGAATACAGT TCCTGCCCTG ATGACGCAAT TTTCCAAAGC 
TTGGCTCGCG CGTACTCTTC TTTCAACCCA GTCATGTCTG ACCCCAATCG ACCTCCCTGT 
CGCAAGAATG ACGATGACAG CAGCTTTGTA GATGGAACGA CCAATGGTGG TGCATGGTAC 
AGCGTCCCCG GTGGAATGCA AGACTTCAAT TACCTGAGCA GCAACTGCTT CGAGATCACT 
GTGGAGCTTA GCTGTGAGAA GTTCCCACCG GAAGAGACTC TCAAAAGCTA CTGGGAAGAT 
AACAAAAACT CCCTCATCAG CTACCTGGAG CAGATACACC GAGGTGTTAA AGGGTTTGTC 
CGTGACCTTC AGGGTAACCC GATTGCCAAC GCAACCATCT CTGTGGACGG GATAGACCAT 
GATGTCACCT CGGCTAAGGA TGGGGATTAC TGGCGATTGC TTGCTCCTGG AAACTATAAA 
CTTACAGCCT CCGCTCCTGG CTACCTGGCA ATCACAAAGA AAGTGGCAGT TCCTTTTAGC 
CCTGCTGTTG GGGTGGACTT TGAGCTTGAG TCTTTCTCTG AAAGGAAGGA GGAGGAGAAG 
GAAGAATTGA TGGAGTGGTG GAAAATGATG TCAGAAACTT TGAATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038522.2
CDS	161..1591
Translation	MAGRGGRVLLALCAALVAGGWLLTAEAQEPGAPAAGMRRRRRLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNLKKIVDQNSKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGTAHEYSSCPDDAIFQSLARAYSSFNPVMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKSYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVDGIDHDVTSAKDGDYWRLLAPGNYKLTASAPGYLAITKKVAVPFSPAVGVDFELESFSERKEEEKEELMEWWKMMSETLNF

Target ORF information:

RefSeq Version	NM_013494.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus carboxypeptidase E (Cpe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013494.4

ORF Insert Sequence:

ATGGCCGGGC GCGGAGGACG GGTGCTGCTG GCGCTGTGTG CCGCGCTGGT GGCCGGCGGG 
TGGCTGCTGA CGGCTGAAGC CCAGGAGCCC GGGGCGCCAG CGGCTGGCAT GAGGCGCCGC 
CGGCGGCTCC AGCAAGAGGA CGGCATCTCC TTCGAGTACC ACCGCTATCC AGAGCTGCGC 
GAGGCGCTGG TGTCCGTATG GCTGCAGTGC ACCGCCATCA GCAGAATCTA CACAGTGGGG 
CGCAGCTTCG AGGGCCGGGA GCTCCTGGTC ATCGAGCTGT CTGACAACCC CGGGGTCCAT 
GAGCCGGGTG AACCTGAATT TAAATACATT GGGAACATGC ATGGTAATGA GGCGGTTGGA 
CGGGAACTGC TTATTTTCTT GGCCCAGTAC CTGTGTAACG AGTACCAGAA AGGCAATGAG 
ACAATTGTCA ACCTGATCCA CAGCACCCGA ATTCATATCA TGCCCTCCTT GAACCCCGAC 
GGCTTTGAGA AAGCCGCATC GCAGCCCGGC GAGCTGAAGG ACTGGTTTGT GGGCCGCAGC 
AACGCCCAGG GAATAGATCT GAACCGTAAC TTCCCAGACC TGGACAGGAT CGTGTATGTT 
AATGAGAAAG AAGGCGGTCC TAACAATCAC CTGCTGAAGA ATCTGAAGAA AATTGTGGAC 
CAAAATTCAA AGCTTGCCCC CGAGACCAAG GCTGTCATTC ACTGGATCAT GGACATTCCA 
TTTGTGCTTT CTGCCAATCT GCACGGAGGA GACCTTGTGG CTAATTACCC ATATGATGAG 
ACACGGAGCG GTACTGCTCA CGAATACAGT TCCTGCCCTG ATGACGCAAT TTTCCAAAGC 
TTGGCTCGCG CGTACTCTTC TTTCAACCCA GTCATGTCTG ACCCCAATCG ACCTCCCTGT 
CGCAAGAATG ACGATGACAG CAGCTTTGTA GATGGAACGA CCAATGGTGG TGCATGGTAC 
AGCGTCCCCG GTGGAATGCA AGACTTCAAT TACCTGAGCA GCAACTGCTT CGAGATCACT 
GTGGAGCTTA GCTGTGAGAA GTTCCCACCG GAAGAGACTC TCAAAAGCTA CTGGGAAGAT 
AACAAAAACT CCCTCATCAG CTACCTGGAG CAGATACACC GAGGTGTTAA AGGGTTTGTC 
CGTGACCTTC AGGGTAACCC GATTGCCAAC GCAACCATCT CTGTGGACGG GATAGACCAT 
GATGTCACCT CGGCTAAGGA TGGGGATTAC TGGCGATTGC TTGCTCCTGG AAACTATAAA 
CTTACAGCCT CCGCTCCTGG CTACCTGGCA ATCACAAAGA AAGTGGCAGT TCCTTTTAGC 
CCTGCTGTTG GGGTGGACTT TGAGCTTGAG TCTTTCTCTG AAAGGAAGGA GGAGGAGAAG 
GAAGAATTGA TGGAGTGGTG GAAAATGATG TCAGAAACTT TGAATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Augmented Responses to Ozone in Obese Mice Require IL-17A and Gastrin-releasing Peptide.
American journal of respiratory cell and molecular biology(2017 Sep)
Mathews JA,Krishnamoorthy N,Kasahara DI,Hutchinson J,Cho Y,Brand JD,Williams AS,Wurmbrand AP,Ribeiro L,Cuttitta F,Sunday ME,Levy BD,Shore SA

Absence of Carboxypeptidase E/Neurotrophic Factor-?1 in Knock-Out Mice Leads to Dysfunction of BDNF-TRKB Signaling in Hippocampus.
Journal of molecular neuroscience : MN62(1)79-87(2017 May)
Xiao L,Chang SY,Xiong ZG,Selveraj P,Peng Loh Y

Neurotrophic Factor-?1: A Key Wnt-?-Catenin Dependent Anti-Proliferation Factor and ERK-Sox9 Activated Inducer of Embryonic Neural Stem Cell Differentiation to Astrocytes in Neurodevelopment.
Stem cells (Dayton, Ohio)35(3)557-571(2017 Mar)
Selvaraj P,Xiao L,Lee C,Murthy SR,Cawley NX,Lane M,Merchenthaler I,Ahn S,Loh YP

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

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Cpe cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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