Cpa3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpa3
Entrez Gene ID	12873
Full Name	carboxypeptidase A3, mast cell
Synonyms	MC-CPA
General protein information	Preferred Names carboxypeptidase A3, mast cell Names mast cell carboxypeptidase A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 A2\|3 6.25 cM
Summary	This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases and preproprotein that is proteolytically processed to generate a mature protein product. This product is released by mast cells and may be involved in the degradation of endogenous proteins and the inactivation of venom-associated peptides. Homozygous knockout mice for this gene exhibit impaired mast cell development. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006535382.3	XP_006535445.1	mast cell carboxypeptidase A isoform X1
NM_007753.2	NP_031779.1	mast cell carboxypeptidase A preproprotein
XM_006535382.4	XP_006535445.1	mast cell carboxypeptidase A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CPA3	NP_001861.2
Bos taurus (cattle)	CPA3	NP_001179589.1
Magnaporthe oryzae (rice blast fungus)	MGG_06906	XP_003709640.1
	CPA3	XP_001110081.2
Canis lupus familiaris (dog)	CPA3	XP_542828.2
Kluyveromyces lactis	KLLA0F27929g	XP_456322.1
Neurospora crassa	NCU01464	XP_956741.1
Pan troglodytes (chimpanzee)	CPA3	XP_526465.2
Rattus norvegicus (Norway rat)	Cpa3	NP_062173.1
Saccharomyces cerevisiae (baker's yeast)	ECM14	NP_012000.1
Xenopus tropicalis (tropical clawed frog)	cpb1	XP_002937384.2
Mus musculus (house mouse)	Cpa3	NP_031779.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013181	XP_003437177.1
Eremothecium gossypii	AGOS_AFR045W	NP_985592.1
Schizosaccharomyces pombe (fission yeast)	SPBC337.07c	NP_595408.1

Gene Ontology
Bibliography

Related articles in PubMed

Mast cell hyperactivity underpins the development of oxygen-induced retinopathy.
Matsuda K, Okamoto N, Kondo M, Arkwright PD, Karasawa K, Ishizaka S, Yokota S, Matsuda A, Jung K, Oida K, Amagai Y, Jang H, Noda E, Kakinuma R, Yasui K, Kaku U, Mori Y, Onai N, Ohteki T, Tanaka A, Matsuda H
The Journal of clinical investigation127(11)3987-4000(2017 Nov)

The neuropeptide neuromedin U stimulates innate lymphoid cells and type 2 inflammation.
Klose CSN, Mahlak?iv T, Moeller JB, Rankin LC, Flamar AL, Kabata H, Monticelli LA, Moriyama S, Putzel GG, Rakhilin N, Shen X, Kostenis E, K?nig GM, Senda T, Carpenter D, Farber DL, Artis D
Nature549(7671)282-286(2017 09)

Megakaryocytes compensate for Kit insufficiency in murine arthritis.
Cunin P, Penke LR, Thon JN, Monach PA, Jones T, Chang MH, Chen MM, Melki I, Lacroix S, Iwakura Y, Ware J, Gurish MF, Italiano JE, Boilard E, Nigrovic PA
The Journal of clinical investigation127(5)1714-1724(2017 May)

Defective bone repair in mast cell-deficient Cpa3Cre/+ mice.
Ramirez-GarciaLuna JL, Chan D, Samberg R, Abou-Rjeili M, Wong TH, Li A, Feyerabend TB, Rodewald HR, Henderson JE, Martineau PA
PloS one12(3)e0174396(2017)

Abundant expression of transcription factor GATA-2 in proliferating but not in differentiated mast cells in tissues of mice: demonstration by in situ hybridization.
Jippo T, Mizuno H, Xu Z, Nomura S, Yamamoto M, Kitamura Y
Blood87(3)993-8(1996 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mast cells in experimental arthritis contained high levels of mMC-CPA mRNA, and also lacked the exopeptidase.
Title: Experimental Arthritis Is Dependent on Mouse Mast Cell Protease-5.

The goal of the current study was to compare bone repair in normal wild type and Cpa3Cre/+ mice, which lack mast cells in the absence of any other hematopoietic or non- hematopoietic deficiencies. Mast cells appear in healing bone of wild type mice but not Cpa3Cre/+ mice, beginning 14 days after surgery. Re-vascularization of repair tissue and deposition of mineralized bone was delayed and dis-organised in Cpa3Cre/+ mice
Title: Defective bone repair in mast cell-deficient Cpa3Cre/+ mice.

Data suggest that carboxypeptidase A3 (Cpa3)-Cre; myeloid cell leukemia sequence 1 (Mcl-1)(fl/fl) mice may be useful in analyzing the roles of mast cells and basophils in health and disease.
Title: Reduced mast cell and basophil numbers and function in Cpa3-Cre; Mcl-1fl/fl mice.

Mc-cpa plays a crucial role for the generation of phenotypically mature mast cells.
Title: Loss of histochemical identity in mast cells lacking carboxypeptidase A.

cathepsin E is located in mast-cell secretory granules in complex with heparin proteoglycans, and has a role in the processing of procarboxypeptidase A into active protease
Title: A role for cathepsin E in the processing of mast-cell carboxypeptidase A.

The following Cpa3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpa3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19120	NM_007753.2 Latest version!	Mus musculus carboxypeptidase A3, mast cell (Cpa3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu31309	XM_006535382.4 Latest version!	Mus musculus carboxypeptidase A3, mast cell (Cpa3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu31309	XM_006535382.3	Mus musculus carboxypeptidase A3, mast cell (Cpa3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpa3 ( NM_007753.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031779.1 Mus musculus carboxypeptidase A3, mast cell (Cpa3), mRNA.

Price & Availability↑

CloneID	OMu19120
Clone ID Related Accession (Same CDS sequence)	NM_007753.2
Accession Version	NM_007753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	mast cell carboxypeptidase A preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY113342.1, J05118.1 and BY584193.1. On Apr 1, 2010 this sequence version replaced NM_007753.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK088906.1, J05118.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGTTCT TCTTGCTTAT GGCTGTCATC TACACTACAC TTGCAATTGC TCCTGTCCAC 
TTTGACAGGG AGAAGGTATT CCGTGTGAAA CTCCAGAATG AAAAGCACGC CAGTGTCTTG 
AAGAACTTGA CCCAAAGCAT TGAGCTCGAC TTCTGGTATC CAGATGCTAT CCATGATATT 
GCTGTGAATA TGACTGTGGA TTTCCGAGTT AGTGAGAAGG AATCTCAGAC CATCCAGTCA 
ACCTTGGAAC AACATAAAAT CCATTATGAA ATTCTGATAC ATGACCTACA AGAAGAGATA 
GAGAAACAGT TTGATGTGAA AGATGAAATC GCAGGCAGGC ACAGTTATGC AAAATACAAT 
GACTGGGACA AGATTGTTTC TTGGACTGAA AAAATGTTAG AAAAACATCC TGAGATGGTT 
TCAAGGATTA AAATTGGATC AACTGTTGAA GATAATCCAT TATATGTCCT CAAGATTGGG 
AAAAAAGATG GAGAAAGAAA GGCTATCTTT ATGGACTGTG GCATTCATGC ACGTGAATGG 
ATTTCTCCAG CTTTCTGTCA GTGGTTTGTC TATCAGGCAA CCAAGAGTTA TGGAAAAAAT 
AAAATCATGA CCAAACTCTT GGACCGGATG AATTTTTACG TTCTGCCTGT ATTCAATGTA 
GACGGATACA TTTGGTCATG GACACAGGAT CGAATGTGGA GAAAAAACCG TTCCAGGAAC 
CAAAACTCCA CCTGCATTGG CACTGACCTC AACAGGAACT TTGATGTCTC GTGGGACTCT 
TCTCCAAACA CCAACAAACC ATGCCTCAAT GTCTACAGGG GACCTGCACC AGAGTCTGAG 
AAAGAGACAA AAGCTGTCAC CAACTTCATT CGAAGCCATC TGAACTCAAT CAAGGCTTAT 
ATCACTTTCC ATTCCTACTC GCAGATGCTA TTAATTCCTT ATGGCTACAC ATTCAAACTG 
CCTCCTAACC ACCAGGACCT ATTGAAAGTT GCAAGGATTG CCACGGATGC TCTCTCAACT 
AGATATGAAA CCCGTTACAT CTACGGCCCA ATAGCATCCA CAATTTACAA GACTTCAGGC 
TCTTCCTTAG ACTGGGTTTA TGACCTGGGC ATCAAACACA CATTTGCCTT TGAGCTTCGT 
GATAAAGGAA AGTCTGGGTT TCTTCTTCCA GAATCCCGGA TAAAGCCAAC ATGTAAAGAA 
ACCATGCTTT CTGTCAAATT TATTGCCAAG TATATCCTCA AGAATACTTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031779.1
CDS	11..1264
Translation	MRFFLLMAVIYTTLAIAPVHFDREKVFRVKLQNEKHASVLKNLTQSIELDFWYPDAIHDIAVNMTVDFRVSEKESQTIQSTLEQHKIHYEILIHDLQEEIEKQFDVKDEIAGRHSYAKYNDWDKIVSWTEKMLEKHPEMVSRIKIGSTVEDNPLYVLKIGKKDGERKAIFMDCGIHAREWISPAFCQWFVYQATKSYGKNKIMTKLLDRMNFYVLPVFNVDGYIWSWTQDRMWRKNRSRNQNSTCIGTDLNRNFDVSWDSSPNTNKPCLNVYRGPAPESEKETKAVTNFIRSHLNSIKAYITFHSYSQMLLIPYGYTFKLPPNHQDLLKVARIATDALSTRYETRYIYGPIASTIYKTSGSSLDWVYDLGIKHTFAFELRDKGKSGFLLPESRIKPTCKETMLSVKFIAKYILKNTS

Target ORF information:

RefSeq Version	NM_007753.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus carboxypeptidase A3, mast cell (Cpa3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007753.2

ORF Insert Sequence:

ATGAGGTTCT TCTTGCTTAT GGCTGTCATC TACACTACAC TTGCAATTGC TCCTGTCCAC 
TTTGACAGGG AGAAGGTATT CCGTGTGAAA CTCCAGAATG AAAAGCACGC CAGTGTCTTG 
AAGAACTTGA CCCAAAGCAT TGAGCTCGAC TTCTGGTATC CAGATGCTAT CCATGATATT 
GCTGTGAATA TGACTGTGGA TTTCCGAGTT AGTGAGAAGG AATCTCAGAC CATCCAGTCA 
ACCTTGGAAC AACATAAAAT CCATTATGAA ATTCTGATAC ATGACCTACA AGAAGAGATA 
GAGAAACAGT TTGATGTGAA AGATGAAATC GCAGGCAGGC ACAGTTATGC AAAATACAAT 
GACTGGGACA AGATTGTTTC TTGGACTGAA AAAATGTTAG AAAAACATCC TGAGATGGTT 
TCAAGGATTA AAATTGGATC AACTGTTGAA GATAATCCAT TATATGTCCT CAAGATTGGG 
AAAAAAGATG GAGAAAGAAA GGCTATCTTT ATGGACTGTG GCATTCATGC ACGTGAATGG 
ATTTCTCCAG CTTTCTGTCA GTGGTTTGTC TATCAGGCAA CCAAGAGTTA TGGAAAAAAT 
AAAATCATGA CCAAACTCTT GGACCGGATG AATTTTTACG TTCTGCCTGT ATTCAATGTA 
GACGGATACA TTTGGTCATG GACACAGGAT CGAATGTGGA GAAAAAACCG TTCCAGGAAC 
CAAAACTCCA CCTGCATTGG CACTGACCTC AACAGGAACT TTGATGTCTC GTGGGACTCT 
TCTCCAAACA CCAACAAACC ATGCCTCAAT GTCTACAGGG GACCTGCACC AGAGTCTGAG 
AAAGAGACAA AAGCTGTCAC CAACTTCATT CGAAGCCATC TGAACTCAAT CAAGGCTTAT 
ATCACTTTCC ATTCCTACTC GCAGATGCTA TTAATTCCTT ATGGCTACAC ATTCAAACTG 
CCTCCTAACC ACCAGGACCT ATTGAAAGTT GCAAGGATTG CCACGGATGC TCTCTCAACT 
AGATATGAAA CCCGTTACAT CTACGGCCCA ATAGCATCCA CAATTTACAA GACTTCAGGC 
TCTTCCTTAG ACTGGGTTTA TGACCTGGGC ATCAAACACA CATTTGCCTT TGAGCTTCGT 
GATAAAGGAA AGTCTGGGTT TCTTCTTCCA GAATCCCGGA TAAAGCCAAC ATGTAAAGAA 
ACCATGCTTT CTGTCAAATT TATTGCCAAG TATATCCTCA AGAATACTTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cpa3 ( XM_006535382.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535445.1 Mus musculus carboxypeptidase A3, mast cell (Cpa3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31309
Clone ID Related Accession (Same CDS sequence)	XM_006535382.3 , XM_006535382.4
Accession Version	XM_006535382.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mast cell carboxypeptidase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCTTGCTTAT GGCTGTCATC TACACTACAC TTGCAATTGC TCCTGTCCAC 
TTTGACAGGG AGAAGGTATT CCGTGTGAAA CTCCAGAATG AAAAGCACGC CAGTGTCTTG 
AAGAACTTGA CCCAAAGCAT TGAGCTCGAC TTCTGGTATC CAGATGCTAT CCATGATATT 
GCTGTGAATA TGACTGTGGA TTTCCGAGTT AGTGAGAAGG AATCTCAGAC CATCCAGTCA 
ACCTTGGAAC AACATAAAAT CCATTATGAA ATTCTGATAC ATGACCTACA AGAAGAGATA 
GAGAAACAGT TTGATGTGAA AGATGAAATC GCAGGCAGGC ACAGTTATGC AAAATACAAT 
GACTGGGACA AGATTGTTTC TTGGACTGAA AAAATGTTAG AAAAACATCC TGAGATGGTT 
TCAAGGATTA AAATTGGATC AACTGTTGAA GATAATCCAT TATATGTCCT CAAGATTGGG 
AAAAAAGATG GAGAAAGAAA GGCTATCTTT ATGGACTGTG GCATTCATGC ACGTGAATGG 
ATTTCTCCAG CTTTCTGTCA GTGGTTTGTC TATCAGGCAA CCAAGAGTTA TGGAAAAAAT 
AAAATCATGA CCAAACTCTT GGACCGGATG AATTTTTACG TTCTGCCTGT ATTCAATGTA 
GACGGATACA TTTGGTCATG GACACAGGAT CGAATGTGGA GAAAAAACCG TTCCAGGAAC 
CAAAACTCCA CCTGCATTGG CACTGACCTC AACAGGAACT TTGATGTCTC GTGGGACTCT 
TCTCCAAACA CCAACAAACC ATGCCTCAAT GTCTACAGGG GACCTGCACC AGAGTCTGAG 
AAAGAGACAA AAGCTGTCAC CAACTTCATT CGAAGCCATC TGAACTCAAT CAAGGCTTAT 
ATCACTTTCC ATTCCTACTC GCAGATGCTA TTAATTCCTT ATGGCTACAC ATTCAAACTG 
CCTCCTAACC ACCAGGACCT AACAAGACTT CAGGCTCTTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535445.1
CDS	39..1043
Translation	MRFFLLMAVIYTTLAIAPVHFDREKVFRVKLQNEKHASVLKNLTQSIELDFWYPDAIHDIAVNMTVDFRVSEKESQTIQSTLEQHKIHYEILIHDLQEEIEKQFDVKDEIAGRHSYAKYNDWDKIVSWTEKMLEKHPEMVSRIKIGSTVEDNPLYVLKIGKKDGERKAIFMDCGIHAREWISPAFCQWFVYQATKSYGKNKIMTKLLDRMNFYVLPVFNVDGYIWSWTQDRMWRKNRSRNQNSTCIGTDLNRNFDVSWDSSPNTNKPCLNVYRGPAPESEKETKAVTNFIRSHLNSIKAYITFHSYSQMLLIPYGYTFKLPPNHQDLTRLQALP

Target ORF information:

RefSeq Version	XM_006535382.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus carboxypeptidase A3, mast cell (Cpa3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535382.3

ORF Insert Sequence:

ATGAGGTTCT TCTTGCTTAT GGCTGTCATC TACACTACAC TTGCAATTGC TCCTGTCCAC 
TTTGACAGGG AGAAGGTATT CCGTGTGAAA CTCCAGAATG AAAAGCACGC CAGTGTCTTG 
AAGAACTTGA CCCAAAGCAT TGAGCTCGAC TTCTGGTATC CAGATGCTAT CCATGATATT 
GCTGTGAATA TGACTGTGGA TTTCCGAGTT AGTGAGAAGG AATCTCAGAC CATCCAGTCA 
ACCTTGGAAC AACATAAAAT CCATTATGAA ATTCTGATAC ATGACCTACA AGAAGAGATA 
GAGAAACAGT TTGATGTGAA AGATGAAATC GCAGGCAGGC ACAGTTATGC AAAATACAAT 
GACTGGGACA AGATTGTTTC TTGGACTGAA AAAATGTTAG AAAAACATCC TGAGATGGTT 
TCAAGGATTA AAATTGGATC AACTGTTGAA GATAATCCAT TATATGTCCT CAAGATTGGG 
AAAAAAGATG GAGAAAGAAA GGCTATCTTT ATGGACTGTG GCATTCATGC ACGTGAATGG 
ATTTCTCCAG CTTTCTGTCA GTGGTTTGTC TATCAGGCAA CCAAGAGTTA TGGAAAAAAT 
AAAATCATGA CCAAACTCTT GGACCGGATG AATTTTTACG TTCTGCCTGT ATTCAATGTA 
GACGGATACA TTTGGTCATG GACACAGGAT CGAATGTGGA GAAAAAACCG TTCCAGGAAC 
CAAAACTCCA CCTGCATTGG CACTGACCTC AACAGGAACT TTGATGTCTC GTGGGACTCT 
TCTCCAAACA CCAACAAACC ATGCCTCAAT GTCTACAGGG GACCTGCACC AGAGTCTGAG 
AAAGAGACAA AAGCTGTCAC CAACTTCATT CGAAGCCATC TGAACTCAAT CAAGGCTTAT 
ATCACTTTCC ATTCCTACTC GCAGATGCTA TTAATTCCTT ATGGCTACAC ATTCAAACTG 
CCTCCTAACC ACCAGGACCT AACAAGACTT CAGGCTCTTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cpa3 ( XM_006535382.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535445.1 Mus musculus carboxypeptidase A3, mast cell (Cpa3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31309
Clone ID Related Accession (Same CDS sequence)	XM_006535382.3 , XM_006535382.4
Accession Version	XM_006535382.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mast cell carboxypeptidase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535382.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTCT TCTTGCTTAT GGCTGTCATC TACACTACAC TTGCAATTGC TCCTGTCCAC 
TTTGACAGGG AGAAGGTATT CCGTGTGAAA CTCCAGAATG AAAAGCACGC CAGTGTCTTG 
AAGAACTTGA CCCAAAGCAT TGAGCTCGAC TTCTGGTATC CAGATGCTAT CCATGATATT 
GCTGTGAATA TGACTGTGGA TTTCCGAGTT AGTGAGAAGG AATCTCAGAC CATCCAGTCA 
ACCTTGGAAC AACATAAAAT CCATTATGAA ATTCTGATAC ATGACCTACA AGAAGAGATA 
GAGAAACAGT TTGATGTGAA AGATGAAATC GCAGGCAGGC ACAGTTATGC AAAATACAAT 
GACTGGGACA AGATTGTTTC TTGGACTGAA AAAATGTTAG AAAAACATCC TGAGATGGTT 
TCAAGGATTA AAATTGGATC AACTGTTGAA GATAATCCAT TATATGTCCT CAAGATTGGG 
AAAAAAGATG GAGAAAGAAA GGCTATCTTT ATGGACTGTG GCATTCATGC ACGTGAATGG 
ATTTCTCCAG CTTTCTGTCA GTGGTTTGTC TATCAGGCAA CCAAGAGTTA TGGAAAAAAT 
AAAATCATGA CCAAACTCTT GGACCGGATG AATTTTTACG TTCTGCCTGT ATTCAATGTA 
GACGGATACA TTTGGTCATG GACACAGGAT CGAATGTGGA GAAAAAACCG TTCCAGGAAC 
CAAAACTCCA CCTGCATTGG CACTGACCTC AACAGGAACT TTGATGTCTC GTGGGACTCT 
TCTCCAAACA CCAACAAACC ATGCCTCAAT GTCTACAGGG GACCTGCACC AGAGTCTGAG 
AAAGAGACAA AAGCTGTCAC CAACTTCATT CGAAGCCATC TGAACTCAAT CAAGGCTTAT 
ATCACTTTCC ATTCCTACTC GCAGATGCTA TTAATTCCTT ATGGCTACAC ATTCAAACTG 
CCTCCTAACC ACCAGGACCT AACAAGACTT CAGGCTCTTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535445.1
CDS	39..1043
Translation	MRFFLLMAVIYTTLAIAPVHFDREKVFRVKLQNEKHASVLKNLTQSIELDFWYPDAIHDIAVNMTVDFRVSEKESQTIQSTLEQHKIHYEILIHDLQEEIEKQFDVKDEIAGRHSYAKYNDWDKIVSWTEKMLEKHPEMVSRIKIGSTVEDNPLYVLKIGKKDGERKAIFMDCGIHAREWISPAFCQWFVYQATKSYGKNKIMTKLLDRMNFYVLPVFNVDGYIWSWTQDRMWRKNRSRNQNSTCIGTDLNRNFDVSWDSSPNTNKPCLNVYRGPAPESEKETKAVTNFIRSHLNSIKAYITFHSYSQMLLIPYGYTFKLPPNHQDLTRLQALP

Target ORF information:

RefSeq Version	XM_006535382.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus carboxypeptidase A3, mast cell (Cpa3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535382.4

ORF Insert Sequence:

ATGAGGTTCT TCTTGCTTAT GGCTGTCATC TACACTACAC TTGCAATTGC TCCTGTCCAC 
TTTGACAGGG AGAAGGTATT CCGTGTGAAA CTCCAGAATG AAAAGCACGC CAGTGTCTTG 
AAGAACTTGA CCCAAAGCAT TGAGCTCGAC TTCTGGTATC CAGATGCTAT CCATGATATT 
GCTGTGAATA TGACTGTGGA TTTCCGAGTT AGTGAGAAGG AATCTCAGAC CATCCAGTCA 
ACCTTGGAAC AACATAAAAT CCATTATGAA ATTCTGATAC ATGACCTACA AGAAGAGATA 
GAGAAACAGT TTGATGTGAA AGATGAAATC GCAGGCAGGC ACAGTTATGC AAAATACAAT 
GACTGGGACA AGATTGTTTC TTGGACTGAA AAAATGTTAG AAAAACATCC TGAGATGGTT 
TCAAGGATTA AAATTGGATC AACTGTTGAA GATAATCCAT TATATGTCCT CAAGATTGGG 
AAAAAAGATG GAGAAAGAAA GGCTATCTTT ATGGACTGTG GCATTCATGC ACGTGAATGG 
ATTTCTCCAG CTTTCTGTCA GTGGTTTGTC TATCAGGCAA CCAAGAGTTA TGGAAAAAAT 
AAAATCATGA CCAAACTCTT GGACCGGATG AATTTTTACG TTCTGCCTGT ATTCAATGTA 
GACGGATACA TTTGGTCATG GACACAGGAT CGAATGTGGA GAAAAAACCG TTCCAGGAAC 
CAAAACTCCA CCTGCATTGG CACTGACCTC AACAGGAACT TTGATGTCTC GTGGGACTCT 
TCTCCAAACA CCAACAAACC ATGCCTCAAT GTCTACAGGG GACCTGCACC AGAGTCTGAG 
AAAGAGACAA AAGCTGTCAC CAACTTCATT CGAAGCCATC TGAACTCAAT CAAGGCTTAT 
ATCACTTTCC ATTCCTACTC GCAGATGCTA TTAATTCCTT ATGGCTACAC ATTCAAACTG 
CCTCCTAACC ACCAGGACCT AACAAGACTT CAGGCTCTTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mast cell hyperactivity underpins the development of oxygen-induced retinopathy.
The Journal of clinical investigation127(11)3987-4000(2017 Nov)
Matsuda K,Okamoto N,Kondo M,Arkwright PD,Karasawa K,Ishizaka S,Yokota S,Matsuda A,Jung K,Oida K,Amagai Y,Jang H,Noda E,Kakinuma R,Yasui K,Kaku U,Mori Y,Onai N,Ohteki T,Tanaka A,Matsuda H

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Cpa3 cDNA ORF clone, Mus musculus(house mouse)

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ORF Insert Sequence:

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