Col1a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col1a1
Entrez Gene ID	12842
Full Name	collagen, type I, alpha 1
Synonyms	Col1a-1,Cola-1,Cola1,Mov-13,Mov13
General protein information	Preferred Names collagen, type I, alpha 1 Names collagen alpha-1(I) chain alpha-1 type 1 collagen alpha-1 type I collagen procollagen, type I, alpha 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 59.01 cM\|11 D
Summary	This gene encodes the alpha-1 subunit of the fibril-forming type I collagen, the most abundant protein of bone, skin and tendon extracellular matrices. The encoded protein, in association with alpha-2 subunit, forms heterotrimeric type I procollagen that undergoes proteolytic processing during fibril formation. Mice lacking the encoded protein die in utero caused by the rupture of a major blood vessel. Transgenic mice expressing significantly lower levels of this gene exhibit morphological and functional defects in mineralized and non-mineralized connective tissue and, progressive loss of hearing. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007742.4	NP_031768.2	collagen alpha-1(I) chain preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL1A1	NP_001029211.1
Mus musculus (house mouse)	Col1a1	NP_031768.2
Danio rerio (zebrafish)	col1a1a	NP_954684.1
Pan troglodytes (chimpanzee)	COL1A1	XP_001169409.1
	COL1A1	XP_001096194.2
Canis lupus familiaris (dog)	COL1A1	NP_001003090.1
Rattus norvegicus (Norway rat)	Col1a1	NP_445756.1
Homo sapiens (human)	COL1A1	NP_000079.2
Xenopus tropicalis (tropical clawed frog)	LOC496414	NP_001011005.1

Gene Ontology

The following Col1a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col1a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22651	NM_007742.4 Latest version!	Mus musculus collagen, type I, alpha 1 (Col1a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col1a1 ( NM_007742.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031768.2 Mus musculus collagen, type I, alpha 1 (Col1a1), mRNA.

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CloneID	OMu22651
Clone ID Related Accession (Same CDS sequence)	NM_007742.4
Accession Version	NM_007742.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(I) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY133378.1, AK159283.1, AL662790.21, AK159285.1, AL606480.11 and BQ032538.1. On Sep 11, 2015 this sequence version replaced NM_007742.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK159285.1, BC050014.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG GGGCCACTGC CCTCCTGACG 
CATGGCCAAG AAGACATCCC TGAAGTCAGC TGCATACACA ATGGCCTAAG GGTCCCCAAT 
GGTGAGACGT GGAAACCCGA GGTATGCTTG ATCTGTATCT GCCACAATGG CACGGCTGTG 
TGCGATGACG TGCAATGCAA TGAAGAACTG GACTGTCCCA ACCCCCAAAG ACGGGAGGGC 
GAGTGCTGTG CTTTCTGCCC GGAAGAATAC GTATCACCAA ACTCAGAAGA TGTAGGAGTC 
GAGGGACCCA AGGGAGACCC TGGCCCCCAA GGCCCAAGGG GACCCGTTGG CCCCCCTGGA 
CGAGATGGCA TCCCTGGACA GCCTGGACTT CCTGGTCCTC CTGGTCCCCC TGGGCCCCCC 
GGACCCCCTG GCCTTGGAGG AAACTTTGCT TCCCAGATGT CCTATGGCTA TGATGAAAAA 
TCAGCTGGAG TTTCCGTGCC TGGCCCCATG GGTCCTTCTG GTCCTCGTGG TCTCCCTGGC 
CCCCCTGGTG CACCTGGTCC ACAAGGTTTC CAAGGCCCCC CTGGTGAACC TGGCGAGCCT 
GGCGGTTCAG GTCCAATGGG TCCCCGAGGT CCCCCTGGCC CTCCTGGCAA GAATGGAGAT 
GATGGGGAAG CTGGCAAGCC CGGCCGTCCT GGTGAGCGTG GACCTCCTGG ACCTCAGGGT 
GCTCGTGGAT TGCCTGGAAC AGCTGGCCTC CCTGGAATGA AGGGACACCG AGGCTTCAGT 
GGTTTGGATG GTGCCAAAGG AGATGCTGGT CCTGCTGGTC CTAAGGGAGA GCCCGGCAGT 
CCTGGTGAAA ACGGAGCTCC TGGCCAGATG GGTCCCCGAG GTCTGCCCGG TGAGAGAGGT 
CGCCCTGGAC CTCCTGGCAC TGCTGGTGCT CGCGGTAACG ATGGTGCTGT TGGTGCTGCT 
GGACCCCCTG GTCCCACCGG CCCCACTGGC CCTCCTGGCT TCCCTGGTGC AGTTGGTGCT 
AAGGGTGAAG CTGGTCCCCA AGGAGCTAGA GGCTCTGAAG GTCCCCAGGG TGTGCGTGGT 
GAGCCCGGAC CCCCTGGCCC TGCTGGTGCT GCCGGCCCTG CTGGAAACCC TGGTGCTGAT 
GGACAACCTG GCGCTAAAGG TGCCAATGGT GCTCCTGGTA TTGCTGGTGC TCCTGGCTTC 
CCTGGTGCCC GAGGCCCCTC TGGACCCCAG GGCCCCAGCG GCCCTCCAGG TCCCAAGGGT 
AACAGTGGTG AACCTGGTGC TCCTGGCAAC AAAGGAGACA CTGGTGCCAA AGGAGAACCC 
GGTGCTACTG GAGTTCAAGG TCCCCCAGGC CCTGCCGGAG AAGAAGGAAA ACGAGGAGCC 
CGTGGTGAGC CTGGACCTTC CGGACTGCCT GGACCTCCTG GCGAGCGTGG TGGACCTGGT 
AGCCGTGGTT TCCCTGGTGC TGATGGTGTT GCTGGCCCCA AGGGTCCTTC CGGTGAACGT 
GGTGCTCCCG GACCTGCTGG TCCCAAAGGT TCTCCTGGTG AAGCTGGTCG CCCCGGTGAA 
GCTGGTCTCC CTGGTGCCAA GGGTCTCACT GGCAGTCCTG GCAGCCCTGG TCCTGATGGC 
AAAACCGGCC CCCCTGGTCC CGCTGGTCAA GATGGTCGCC CTGGACCCGC AGGTCCTCCT 
GGAGCCCGTG GCCAGGCTGG TGTGATGGGA TTCCCTGGAC CTAAGGGTAC CGCTGGAGAA 
CCTGGAAAGG CTGGAGAGCG AGGCCTTCCC GGACCCCCTG GCGCTGTTGG TCCTGCTGGC 
AAAGATGGAG AAGCTGGAGC TCAGGGAGCC CCTGGCCCTG CTGGTCCTGC TGGTGAGAGA 
GGTGAACAAG GTCCCGCTGG CTCCCCTGGA TTCCAGGGTC TTCCTGGTCC TGCCGGTCCT 
CCTGGTGAAG CAGGCAAGCC TGGTGAACAG GGTGTTCCTG GAGACCTTGG TGCCCCTGGA 
CCCTCTGGCG CAAGAGGCGA GAGAGGTTTC CCTGGTGAAC GTGGTGTACA AGGTCCCCCA 
GGTCCTGCTG GTCCCCGAGG AAACAATGGT GCCCCCGGCA ACGATGGTGC CAAGGGTGAT 
ACTGGTGCCC CCGGAGCTCC CGGTAGCCAG GGTGCCCCCG GTCTTCAGGG AATGCCTGGT 
GAACGTGGTG CAGCTGGTCT TCCAGGTCCT AAGGGTGACA GAGGTGATGC TGGTCCCAAA 
GGTGCTGATG GTTCTCCTGG TAAAGATGGT GCCCGTGGTC TGACTGGTCC CATTGGTCCT 
CCTGGCCCTG CTGGTGCCCC TGGTGACAAG GGTGAAGCTG GTCCCAGTGG TCCTCCCGGT 
CCCACCGGAG CCCGTGGTGC TCCCGGAGAC CGTGGTGAGG CTGGTCCCCC TGGTCCTGCT 
GGCTTTGCCG GCCCCCCTGG TGCTGATGGC CAACCTGGTG CGAAAGGTGA ACCTGGTGAT 
ACTGGTGTTA AAGGTGATGC TGGTCCTCCT GGCCCTGCTG GTCCTGCTGG ACCCCCCGGC 
CCCATTGGTA ACGTTGGTGC TCCTGGACCC AAAGGTCCTC GTGGTGCTGC TGGTCCCCCT 
GGTGCTACTG GCTTCCCTGG TGCTGCTGGC CGTGTCGGTC CCCCTGGTCC CTCTGGAAAT 
GCTGGACCCC CTGGCCCTCC CGGTCCCGTT GGCAAAGAAG GGGGCAAAGG TCCCCGTGGT 
GAGACTGGCC CTGCTGGACG TCCTGGTGAA GTTGGTCCCC CAGGTCCCCC CGGTCCTGCT 
GGTGAGAAAG GATCTCCTGG TGCTGATGGA CCTGCTGGCT CTCCTGGTAC CCCTGGACCT 
CAGGGTATTG CTGGACAACG TGGTGTGGTC GGTCTTCCCG GTCAGAGAGG AGAAAGAGGC 
TTCCCTGGTC TTCCTGGCCC CTCTGGTGAA CCTGGCAAAC AAGGTCCTTC TGGATCAAGT 
GGTGAACGCG GTCCCCCTGG CCCCATGGGG CCCCCTGGAT TGGCTGGTCC CCCTGGTGAA 
TCTGGACGTG AGGGATCCCC TGGTGCTGAA GGCTCCCCTG GAAGGGATGG TGCTCCCGGG 
GCCAAGGGTG ACCGTGGTGA GACTGGCCCC GCTGGCCCCC CTGGTGCCCC TGGTGCTCCC 
GGTGCTCCCG GCCCTGTTGG TCCCGCTGGC AAGAATGGCG ATCGTGGTGA GACTGGTCCT 
GCTGGTCCTG CTGGTCCCAT TGGCCCTGCT GGTGCCCGTG GCCCTGCTGG ACCCCAAGGC 
CCCCGTGGTG ACAAGGGTGA GACAGGCGAA CAAGGTGACA GAGGCATAAA GGGTCATCGT 
GGCTTCTCTG GTCTCCAGGG TCCTCCTGGT TCTCCTGGTT CTCCTGGTGA ACAAGGCCCC 
TCTGGAGCTT CAGGTCCTGC AGGCCCCCGG GGTCCCCCTG GCTCTGCTGG TTCTCCTGGC 
AAAGACGGAC TCAACGGTCT CCCTGGCCCC ATTGGTCCCC CTGGTCCTCG AGGTCGCACT 
GGTGACAGCG GCCCTGCTGG TCCCCCCGGC CCTCCTGGAC CCCCTGGCCC TCCTGGACCT 
CCCAGTGGCG GTTATGACTT CAGCTTCCTG CCTCAGCCAC CTCAAGAGAA GTCTCAAGAT 
GGTGGCCGCT ACTACCGGGC CGATGATGCT AACGTGGTTC GTGACCGTGA CCTTGAGGTG 
GACACCACCC TCAAGAGCCT GAGTCAGCAG ATTGAGAACA TCCGCAGCCC CGAAGGCAGC 
CGCAAGAACC CTGCCCGCAC ATGCCGCGAC CTCAAGATGT GCCACTCTGA CTGGAAGAGC 
GGAGAGTACT GGATCGACCC TAACCAAGGC TGCAACCTGG ACGCCATCAA GGTCTACTGC 
AACATGGAGA CAGGTCAGAC CTGTGTGTTC CCTACTCAGC CGTCTGTGCC TCAGAAGAAC 
TGGTACATCA GCCCGAACCC CAAGGAAAAG AAGCACGTCT GGTTTGGAGA GAGCATGACC 
GATGGATTCC CGTTCGAGTA CGGAAGCGAG GGCTCCGACC CCGCCGATGT CGCTATCCAG 
CTGACCTTCC TGCGCCTAAT GTCCACCGAG GCCTCCCAGA ACATCACCTA TCACTGCAAG 
AACAGCGTAG CCTACATGGA CCAGCAGACT GGCAACCTCA AGAAGGCCCT GCTCCTCCAG 
GGATCCAACG AGATCGAGCT CAGAGGCGAA GGCAACAGTC GCTTCACCTA CAGCACCCTT 
GTGGACGGCT GCACGAGTCA CACCGGAACT TGGGGCAAGA CAGTCATCGA ATACAAAACC 
ACCAAGACCT CCCGCCTGCC CATCATCGAT GTGGCTCCCT TGGACATTGG TGCCCCAGAC 
CAGGAATTCG GACTAGACAT TGGCCCTGCC TGCTTCGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031768.2
CDS	146..4507
Translation	MFSFVDLRLLLLLGATALLTHGQEDIPEVSCIHNGLRVPNGETWKPEVCLICICHNGTAVCDDVQCNEELDCPNPQRREGECCAFCPEEYVSPNSEDVGVEGPKGDPGPQGPRGPVGPPGRDGIPGQPGLPGPPGPPGPPGPPGLGGNFASQMSYGYDEKSAGVSVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGGSGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKGEPGSPGENGAPGQMGPRGLPGERGRPGPPGTAGARGNDGAVGAAGPPGPTGPTGPPGFPGAVGAKGEAGPQGARGSEGPQGVRGEPGPPGPAGAAGPAGNPGADGQPGAKGANGAPGIAGAPGFPGARGPSGPQGPSGPPGPKGNSGEPGAPGNKGDTGAKGEPGATGVQGPPGPAGEEGKRGARGEPGPSGLPGPPGERGGPGSRGFPGADGVAGPKGPSGERGAPGPAGPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGPDGKTGPPGPAGQDGRPGPAGPPGARGQAGVMGFPGPKGTAGEPGKAGERGLPGPPGAVGPAGKDGEAGAQGAPGPAGPAGERGEQGPAGSPGFQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGVQGPPGPAGPRGNNGAPGNDGAKGDTGAPGAPGSQGAPGLQGMPGERGAAGLPGPKGDRGDAGPKGADGSPGKDGARGLTGPIGPPGPAGAPGDKGEAGPSGPPGPTGARGAPGDRGEAGPPGPAGFAGPPGADGQPGAKGEPGDTGVKGDAGPPGPAGPAGPPGPIGNVGAPGPKGPRGAAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPVGKEGGKGPRGETGPAGRPGEVGPPGPPGPAGEKGSPGADGPAGSPGTPGPQGIAGQRGVVGLPGQRGERGFPGLPGPSGEPGKQGPSGSSGERGPPGPMGPPGLAGPPGESGREGSPGAEGSPGRDGAPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAGKNGDRGETGPAGPAGPIGPAGARGPAGPQGPRGDKGETGEQGDRGIKGHRGFSGLQGPPGSPGSPGEQGPSGASGPAGPRGPPGSAGSPGKDGLNGLPGPIGPPGPRGRTGDSGPAGPPGPPGPPGPPGPPSGGYDFSFLPQPPQEKSQDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKSGEYWIDPNQGCNLDAIKVYCNMETGQTCVFPTQPSVPQKNWYISPNPKEKKHVWFGESMTDGFPFEYGSEGSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIELRGEGNSRFTYSTLVDGCTSHTGTWGKTVIEYKTTKTSRLPIIDVAPLDIGAPDQEFGLDIGPACFV

Target ORF information:

RefSeq Version	NM_007742.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type I, alpha 1 (Col1a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007742.4

ORF Insert Sequence:

ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG GGGCCACTGC CCTCCTGACG 
CATGGCCAAG AAGACATCCC TGAAGTCAGC TGCATACACA ATGGCCTAAG GGTCCCCAAT 
GGTGAGACGT GGAAACCCGA GGTATGCTTG ATCTGTATCT GCCACAATGG CACGGCTGTG 
TGCGATGACG TGCAATGCAA TGAAGAACTG GACTGTCCCA ACCCCCAAAG ACGGGAGGGC 
GAGTGCTGTG CTTTCTGCCC GGAAGAATAC GTATCACCAA ACTCAGAAGA TGTAGGAGTC 
GAGGGACCCA AGGGAGACCC TGGCCCCCAA GGCCCAAGGG GACCCGTTGG CCCCCCTGGA 
CGAGATGGCA TCCCTGGACA GCCTGGACTT CCTGGTCCTC CTGGTCCCCC TGGGCCCCCC 
GGACCCCCTG GCCTTGGAGG AAACTTTGCT TCCCAGATGT CCTATGGCTA TGATGAAAAA 
TCAGCTGGAG TTTCCGTGCC TGGCCCCATG GGTCCTTCTG GTCCTCGTGG TCTCCCTGGC 
CCCCCTGGTG CACCTGGTCC ACAAGGTTTC CAAGGCCCCC CTGGTGAACC TGGCGAGCCT 
GGCGGTTCAG GTCCAATGGG TCCCCGAGGT CCCCCTGGCC CTCCTGGCAA GAATGGAGAT 
GATGGGGAAG CTGGCAAGCC CGGCCGTCCT GGTGAGCGTG GACCTCCTGG ACCTCAGGGT 
GCTCGTGGAT TGCCTGGAAC AGCTGGCCTC CCTGGAATGA AGGGACACCG AGGCTTCAGT 
GGTTTGGATG GTGCCAAAGG AGATGCTGGT CCTGCTGGTC CTAAGGGAGA GCCCGGCAGT 
CCTGGTGAAA ACGGAGCTCC TGGCCAGATG GGTCCCCGAG GTCTGCCCGG TGAGAGAGGT 
CGCCCTGGAC CTCCTGGCAC TGCTGGTGCT CGCGGTAACG ATGGTGCTGT TGGTGCTGCT 
GGACCCCCTG GTCCCACCGG CCCCACTGGC CCTCCTGGCT TCCCTGGTGC AGTTGGTGCT 
AAGGGTGAAG CTGGTCCCCA AGGAGCTAGA GGCTCTGAAG GTCCCCAGGG TGTGCGTGGT 
GAGCCCGGAC CCCCTGGCCC TGCTGGTGCT GCCGGCCCTG CTGGAAACCC TGGTGCTGAT 
GGACAACCTG GCGCTAAAGG TGCCAATGGT GCTCCTGGTA TTGCTGGTGC TCCTGGCTTC 
CCTGGTGCCC GAGGCCCCTC TGGACCCCAG GGCCCCAGCG GCCCTCCAGG TCCCAAGGGT 
AACAGTGGTG AACCTGGTGC TCCTGGCAAC AAAGGAGACA CTGGTGCCAA AGGAGAACCC 
GGTGCTACTG GAGTTCAAGG TCCCCCAGGC CCTGCCGGAG AAGAAGGAAA ACGAGGAGCC 
CGTGGTGAGC CTGGACCTTC CGGACTGCCT GGACCTCCTG GCGAGCGTGG TGGACCTGGT 
AGCCGTGGTT TCCCTGGTGC TGATGGTGTT GCTGGCCCCA AGGGTCCTTC CGGTGAACGT 
GGTGCTCCCG GACCTGCTGG TCCCAAAGGT TCTCCTGGTG AAGCTGGTCG CCCCGGTGAA 
GCTGGTCTCC CTGGTGCCAA GGGTCTCACT GGCAGTCCTG GCAGCCCTGG TCCTGATGGC 
AAAACCGGCC CCCCTGGTCC CGCTGGTCAA GATGGTCGCC CTGGACCCGC AGGTCCTCCT 
GGAGCCCGTG GCCAGGCTGG TGTGATGGGA TTCCCTGGAC CTAAGGGTAC CGCTGGAGAA 
CCTGGAAAGG CTGGAGAGCG AGGCCTTCCC GGACCCCCTG GCGCTGTTGG TCCTGCTGGC 
AAAGATGGAG AAGCTGGAGC TCAGGGAGCC CCTGGCCCTG CTGGTCCTGC TGGTGAGAGA 
GGTGAACAAG GTCCCGCTGG CTCCCCTGGA TTCCAGGGTC TTCCTGGTCC TGCCGGTCCT 
CCTGGTGAAG CAGGCAAGCC TGGTGAACAG GGTGTTCCTG GAGACCTTGG TGCCCCTGGA 
CCCTCTGGCG CAAGAGGCGA GAGAGGTTTC CCTGGTGAAC GTGGTGTACA AGGTCCCCCA 
GGTCCTGCTG GTCCCCGAGG AAACAATGGT GCCCCCGGCA ACGATGGTGC CAAGGGTGAT 
ACTGGTGCCC CCGGAGCTCC CGGTAGCCAG GGTGCCCCCG GTCTTCAGGG AATGCCTGGT 
GAACGTGGTG CAGCTGGTCT TCCAGGTCCT AAGGGTGACA GAGGTGATGC TGGTCCCAAA 
GGTGCTGATG GTTCTCCTGG TAAAGATGGT GCCCGTGGTC TGACTGGTCC CATTGGTCCT 
CCTGGCCCTG CTGGTGCCCC TGGTGACAAG GGTGAAGCTG GTCCCAGTGG TCCTCCCGGT 
CCCACCGGAG CCCGTGGTGC TCCCGGAGAC CGTGGTGAGG CTGGTCCCCC TGGTCCTGCT 
GGCTTTGCCG GCCCCCCTGG TGCTGATGGC CAACCTGGTG CGAAAGGTGA ACCTGGTGAT 
ACTGGTGTTA AAGGTGATGC TGGTCCTCCT GGCCCTGCTG GTCCTGCTGG ACCCCCCGGC 
CCCATTGGTA ACGTTGGTGC TCCTGGACCC AAAGGTCCTC GTGGTGCTGC TGGTCCCCCT 
GGTGCTACTG GCTTCCCTGG TGCTGCTGGC CGTGTCGGTC CCCCTGGTCC CTCTGGAAAT 
GCTGGACCCC CTGGCCCTCC CGGTCCCGTT GGCAAAGAAG GGGGCAAAGG TCCCCGTGGT 
GAGACTGGCC CTGCTGGACG TCCTGGTGAA GTTGGTCCCC CAGGTCCCCC CGGTCCTGCT 
GGTGAGAAAG GATCTCCTGG TGCTGATGGA CCTGCTGGCT CTCCTGGTAC CCCTGGACCT 
CAGGGTATTG CTGGACAACG TGGTGTGGTC GGTCTTCCCG GTCAGAGAGG AGAAAGAGGC 
TTCCCTGGTC TTCCTGGCCC CTCTGGTGAA CCTGGCAAAC AAGGTCCTTC TGGATCAAGT 
GGTGAACGCG GTCCCCCTGG CCCCATGGGG CCCCCTGGAT TGGCTGGTCC CCCTGGTGAA 
TCTGGACGTG AGGGATCCCC TGGTGCTGAA GGCTCCCCTG GAAGGGATGG TGCTCCCGGG 
GCCAAGGGTG ACCGTGGTGA GACTGGCCCC GCTGGCCCCC CTGGTGCCCC TGGTGCTCCC 
GGTGCTCCCG GCCCTGTTGG TCCCGCTGGC AAGAATGGCG ATCGTGGTGA GACTGGTCCT 
GCTGGTCCTG CTGGTCCCAT TGGCCCTGCT GGTGCCCGTG GCCCTGCTGG ACCCCAAGGC 
CCCCGTGGTG ACAAGGGTGA GACAGGCGAA CAAGGTGACA GAGGCATAAA GGGTCATCGT 
GGCTTCTCTG GTCTCCAGGG TCCTCCTGGT TCTCCTGGTT CTCCTGGTGA ACAAGGCCCC 
TCTGGAGCTT CAGGTCCTGC AGGCCCCCGG GGTCCCCCTG GCTCTGCTGG TTCTCCTGGC 
AAAGACGGAC TCAACGGTCT CCCTGGCCCC ATTGGTCCCC CTGGTCCTCG AGGTCGCACT 
GGTGACAGCG GCCCTGCTGG TCCCCCCGGC CCTCCTGGAC CCCCTGGCCC TCCTGGACCT 
CCCAGTGGCG GTTATGACTT CAGCTTCCTG CCTCAGCCAC CTCAAGAGAA GTCTCAAGAT 
GGTGGCCGCT ACTACCGGGC CGATGATGCT AACGTGGTTC GTGACCGTGA CCTTGAGGTG 
GACACCACCC TCAAGAGCCT GAGTCAGCAG ATTGAGAACA TCCGCAGCCC CGAAGGCAGC 
CGCAAGAACC CTGCCCGCAC ATGCCGCGAC CTCAAGATGT GCCACTCTGA CTGGAAGAGC 
GGAGAGTACT GGATCGACCC TAACCAAGGC TGCAACCTGG ACGCCATCAA GGTCTACTGC 
AACATGGAGA CAGGTCAGAC CTGTGTGTTC CCTACTCAGC CGTCTGTGCC TCAGAAGAAC 
TGGTACATCA GCCCGAACCC CAAGGAAAAG AAGCACGTCT GGTTTGGAGA GAGCATGACC 
GATGGATTCC CGTTCGAGTA CGGAAGCGAG GGCTCCGACC CCGCCGATGT CGCTATCCAG 
CTGACCTTCC TGCGCCTAAT GTCCACCGAG GCCTCCCAGA ACATCACCTA TCACTGCAAG 
AACAGCGTAG CCTACATGGA CCAGCAGACT GGCAACCTCA AGAAGGCCCT GCTCCTCCAG 
GGATCCAACG AGATCGAGCT CAGAGGCGAA GGCAACAGTC GCTTCACCTA CAGCACCCTT 
GTGGACGGCT GCACGAGTCA CACCGGAACT TGGGGCAAGA CAGTCATCGA ATACAAAACC 
ACCAAGACCT CCCGCCTGCC CATCATCGAT GTGGCTCCCT TGGACATTGG TGCCCCAGAC 
CAGGAATTCG GACTAGACAT TGGCCCTGCC TGCTTCGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Col1a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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