Col9a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col9a2
Entrez Gene ID	12840
Full Name	collagen, type IX, alpha 2
Synonyms	AI427499,Col9a-2
General protein information	Preferred Names collagen, type IX, alpha 2 Names collagen alpha-2(IX) chain procollagen, type IX, alpha 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 D2.2\|4 56.78 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007741.2	NP_031767.2	collagen alpha-2(IX) chain precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL9A2	XP_003949434.1
Canis lupus familiaris (dog)	COL9A2	NP_001184099.1
Xenopus tropicalis (tropical clawed frog)	col9a2	NP_001090661.1
	COL9A2	XP_001082953.2
Bos taurus (cattle)	COL9A2	XP_002686588.2
Mus musculus (house mouse)	Col9a2	NP_031767.2
Rattus norvegicus (Norway rat)	Col9a2	NP_001102145.1
Danio rerio (zebrafish)	col9a2	NP_997744.1
Gallus gallus (chicken)	COL9A2	NP_001258917.1
Homo sapiens (human)	COL9A2	NP_001843.1

Gene Ontology
Bibliography

Related articles in PubMed

Interferon Regulatory Factor 6 Is Necessary for Salivary Glands and Pancreas Development.
Metwalli KA, Do MA, Nguyen K, Mallick S, Kin K, Farokhnia N, Jun G, Fakhouri WD
Journal of dental research97(2)226-236(2018 Feb)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Early changes in morphology, bone mineral density and matrix composition of vertebrae lead to disc degeneration in aged collagen IX -/- mice.
Kamper M, Hamann N, Prein C, Clausen-Schaumann H, Farkas Z, Aszodi A, Niehoff A, Paulsson M, Zaucke F
Matrix biology : journal of the International Society for Matrix Biology49132-143(2016 Jan)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that absence of Col IX induces early developmental, structural and biomechanical alterations in both vertebral body and intervertebral disc which eventually cause severe degenerative changes in the aging spine.
Title: Early changes in morphology, bone mineral density and matrix composition of vertebrae lead to disc degeneration in aged collagen IX -/- mice.

The following Col9a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col9a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00708	NM_007741.2 Latest version!	Mus musculus collagen, type IX, alpha 2 (Col9a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$405.30 $579.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col9a2 ( NM_007741.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031767.2 Mus musculus collagen, type IX, alpha 2 (Col9a2), mRNA.

Price & Availability↑

CloneID	OMu00708
Clone ID Related Accession (Same CDS sequence)	NM_007741.2
Accession Version	NM_007741.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	collagen alpha-2(IX) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY732235.1, BY088510.1, BY232299.1, BU512853.1, CJ046324.1, AA171281.1, CX243824.1, BQ964231.1, CJ312879.1 and BB732275.1. On Apr 21, 2009 this sequence version replaced NM_007741.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC029697.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGACCGCCG TGCCCGCGCC CCGCAGCCTG TTCGTTCTCC TCCAGGTGCT GTGGCTCGCC 
CTGGCTCAGA TCAGAGGTCC ACCAGGGGAG CCGGGTCCTC CAGGGCCACC AGGGCCACCA 
GGAGTGCCTG GTTCAGATGG CATCGACGGT GACAAGGGGC CTCCAGGTAA AGTTGGTCCT 
CCGGGATCCA AAGGAGAGCC TGGCAAACCT GGCCCAGATG GTCCAGATGG GAAACCTGGC 
ATTGATGGTT TAATGGGAGC CAAGGGAGAG CCTGGTCCCG TGGGGACCCC TGGAGTCAAG 
GGCCAGCCTG GGCTCCCAGG GCCCCCTGGC CTGCCGGGCC CTGGTTTTGC TGGACCTCCC 
GGACCCCCTG GACCTGTTGG CCTCCCTGGT GAGATTGGAA CCCCAGGCCC CAAGGGGGAT 
CCAGGACCAG AGGGACCATC AGGGCCCCCA GGGCCCCCTG GGAAACCAGG CCGACCAGGA 
ACCATCCAGG GCTTGGAAGG GAGTGCGGAT TTCTTGTGTC CAACCAACTG TCCAGCTGGT 
GTGAAGGGTC CTCAGGGTTT GCAAGGAGTG AAGGGTCATC CAGGCAAACG GGGGATTCTG 
GGTGATCCTG GTCGCCAGGG GAAGCCAGGT CCCAAGGGAG ATGTGGGTGC CTCTGGAGAG 
CAAGGCATCC CTGGACCACC GGGCCCCCAA GGCATCAGGG GCTACCCGGG CATGGCAGGA 
CCCAAGGGAG AGATGGGTCC TCGTGGCTAT AAAGGCATGG TGGGCTCCAT CGGGGCTGCT 
GGGCCACCGG GTGAAGAAGG CCCAAGAGGG CCACCAGGCC GAGCCGGTGA GAAGGGGGAT 
GTGGGGAGCC AAGGTGCCAG AGGACCCCAG GGGATAACAG GCCCAAAGGG AACAACCGGT 
CCACCAGGCA TTGATGGCAA GGATGGGACC CCAGGCATTC CTGGCATGAA GGGCAGTGCA 
GGACAAGTGG GACGGCCAGG AAGCCCAGGC CACCAGGGCT TAGCGGGTGT GCCGGGTCAG 
CCTGGAACAA AAGGAGGTCC TGGAGACAAG GGTGAACCAG GCCAGCAGGG CTTGCCGGGA 
GTCTCTGGTC CCCCTGGGAA AGAAGGGGAG CCAGGGCCTC GAGGAGAAAT TGGTCCACAG 
GGCATCATGG GACAGAAGGG TGACCAGGGC GAGAGGGGGC CAGTGGGACA GCCAGGCCCT 
CAAGGACGAC AGGGCCCCAA AGGAGAGCAG GGCCCTCCAG GAATTCCAGG ACCCCAAGGC 
TTGCCAGGCA TCAAAGGAGA TAAGGGTTCC CCAGGGAAGA CCGGGCCCCG AGGCGGAGTG 
GGTGACCCGG GGGTGGCCGG CCTCCCGGGA GAGAAAGGAG AGAAGGGCCA ATCAGGCGAG 
CCAGGGCTTA AGGGACAGCA AGGAGTCCGT GGAGAGACCG GCTACCCCGG CCCCAGCGGA 
GATGCCGGTG CCCCAGGAGT GCAGGGCTAC CCCGGGCTTC CCGGGCCCCG AGGACTGGTG 
GGAGATCGAG GCGTGCCAGG ACAACCCGGG AGACAGGGTG TGGTGGGCCG AGCCGCCAGT 
GACCAGCACA TCGTGGATGT GGTGCTGAAG ATGATTCAAG AGCAACTTGC TGAGGTAGCT 
GTGAGTGCCA AGCGAGAAGC CCTGGGTGCA GCAGGGATGG TGGGCCTTCC AGGACCTCCT 
GGGCCCCCTG GATATCCAGG CAAACAGGGG CCCAATGGGC ATCCCGGCCC CCGAGGCATT 
CCTGGCATCG TGGGAGCAGT GGGTCAGATT GGCAACACTG GACCCAAGGG AAAGCGTGGA 
GAGAAGGGGG ATCGAGGAGA AATGGGTCGT GGCCATCCCG GGATGCCTGG GCCCCCAGGG 
ATCCCAGGTC TTCCTGGCCG GCCTGGCCAG GCAATCAATG GCAAGGATGG GGACCGAGGA 
TCCCCAGGGG CTCCAGGAGA GGCTGGGAGA CCTGGCCGGC CAGGCCCAGT GGGGCTACCA 
GGTTTCTGTG AGCCTGCCGC ATGCCTGGGG GCTTCAGCCT ATACCTCTGC TCGCCTCACA 
GAGCCTGGGT CCATCAAGGG GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031767.2
CDS	150..2216
Translation	MTAVPAPRSLFVLLQVLWLALAQIRGPPGEPGPPGPPGPPGVPGSDGIDGDKGPPGKVGPPGSKGEPGKPGPDGPDGKPGIDGLMGAKGEPGPVGTPGVKGQPGLPGPPGLPGPGFAGPPGPPGPVGLPGEIGTPGPKGDPGPEGPSGPPGPPGKPGRPGTIQGLEGSADFLCPTNCPAGVKGPQGLQGVKGHPGKRGILGDPGRQGKPGPKGDVGASGEQGIPGPPGPQGIRGYPGMAGPKGEMGPRGYKGMVGSIGAAGPPGEEGPRGPPGRAGEKGDVGSQGARGPQGITGPKGTTGPPGIDGKDGTPGIPGMKGSAGQVGRPGSPGHQGLAGVPGQPGTKGGPGDKGEPGQQGLPGVSGPPGKEGEPGPRGEIGPQGIMGQKGDQGERGPVGQPGPQGRQGPKGEQGPPGIPGPQGLPGIKGDKGSPGKTGPRGGVGDPGVAGLPGEKGEKGQSGEPGLKGQQGVRGETGYPGPSGDAGAPGVQGYPGLPGPRGLVGDRGVPGQPGRQGVVGRAASDQHIVDVVLKMIQEQLAEVAVSAKREALGAAGMVGLPGPPGPPGYPGKQGPNGHPGPRGIPGIVGAVGQIGNTGPKGKRGEKGDRGEMGRGHPGMPGPPGIPGLPGRPGQAINGKDGDRGSPGAPGEAGRPGRPGPVGLPGFCEPAACLGASAYTSARLTEPGSIKGP

Target ORF information:

RefSeq Version	NM_007741.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IX, alpha 2 (Col9a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007741.2

ORF Insert Sequence:

ATGACCGCCG TGCCCGCGCC CCGCAGCCTG TTCGTTCTCC TCCAGGTGCT GTGGCTCGCC 
CTGGCTCAGA TCAGAGGTCC ACCAGGGGAG CCGGGTCCTC CAGGGCCACC AGGGCCACCA 
GGAGTGCCTG GTTCAGATGG CATCGACGGT GACAAGGGGC CTCCAGGTAA AGTTGGTCCT 
CCGGGATCCA AAGGAGAGCC TGGCAAACCT GGCCCAGATG GTCCAGATGG GAAACCTGGC 
ATTGATGGTT TAATGGGAGC CAAGGGAGAG CCTGGTCCCG TGGGGACCCC TGGAGTCAAG 
GGCCAGCCTG GGCTCCCAGG GCCCCCTGGC CTGCCGGGCC CTGGTTTTGC TGGACCTCCC 
GGACCCCCTG GACCTGTTGG CCTCCCTGGT GAGATTGGAA CCCCAGGCCC CAAGGGGGAT 
CCAGGACCAG AGGGACCATC AGGGCCCCCA GGGCCCCCTG GGAAACCAGG CCGACCAGGA 
ACCATCCAGG GCTTGGAAGG GAGTGCGGAT TTCTTGTGTC CAACCAACTG TCCAGCTGGT 
GTGAAGGGTC CTCAGGGTTT GCAAGGAGTG AAGGGTCATC CAGGCAAACG GGGGATTCTG 
GGTGATCCTG GTCGCCAGGG GAAGCCAGGT CCCAAGGGAG ATGTGGGTGC CTCTGGAGAG 
CAAGGCATCC CTGGACCACC GGGCCCCCAA GGCATCAGGG GCTACCCGGG CATGGCAGGA 
CCCAAGGGAG AGATGGGTCC TCGTGGCTAT AAAGGCATGG TGGGCTCCAT CGGGGCTGCT 
GGGCCACCGG GTGAAGAAGG CCCAAGAGGG CCACCAGGCC GAGCCGGTGA GAAGGGGGAT 
GTGGGGAGCC AAGGTGCCAG AGGACCCCAG GGGATAACAG GCCCAAAGGG AACAACCGGT 
CCACCAGGCA TTGATGGCAA GGATGGGACC CCAGGCATTC CTGGCATGAA GGGCAGTGCA 
GGACAAGTGG GACGGCCAGG AAGCCCAGGC CACCAGGGCT TAGCGGGTGT GCCGGGTCAG 
CCTGGAACAA AAGGAGGTCC TGGAGACAAG GGTGAACCAG GCCAGCAGGG CTTGCCGGGA 
GTCTCTGGTC CCCCTGGGAA AGAAGGGGAG CCAGGGCCTC GAGGAGAAAT TGGTCCACAG 
GGCATCATGG GACAGAAGGG TGACCAGGGC GAGAGGGGGC CAGTGGGACA GCCAGGCCCT 
CAAGGACGAC AGGGCCCCAA AGGAGAGCAG GGCCCTCCAG GAATTCCAGG ACCCCAAGGC 
TTGCCAGGCA TCAAAGGAGA TAAGGGTTCC CCAGGGAAGA CCGGGCCCCG AGGCGGAGTG 
GGTGACCCGG GGGTGGCCGG CCTCCCGGGA GAGAAAGGAG AGAAGGGCCA ATCAGGCGAG 
CCAGGGCTTA AGGGACAGCA AGGAGTCCGT GGAGAGACCG GCTACCCCGG CCCCAGCGGA 
GATGCCGGTG CCCCAGGAGT GCAGGGCTAC CCCGGGCTTC CCGGGCCCCG AGGACTGGTG 
GGAGATCGAG GCGTGCCAGG ACAACCCGGG AGACAGGGTG TGGTGGGCCG AGCCGCCAGT 
GACCAGCACA TCGTGGATGT GGTGCTGAAG ATGATTCAAG AGCAACTTGC TGAGGTAGCT 
GTGAGTGCCA AGCGAGAAGC CCTGGGTGCA GCAGGGATGG TGGGCCTTCC AGGACCTCCT 
GGGCCCCCTG GATATCCAGG CAAACAGGGG CCCAATGGGC ATCCCGGCCC CCGAGGCATT 
CCTGGCATCG TGGGAGCAGT GGGTCAGATT GGCAACACTG GACCCAAGGG AAAGCGTGGA 
GAGAAGGGGG ATCGAGGAGA AATGGGTCGT GGCCATCCCG GGATGCCTGG GCCCCCAGGG 
ATCCCAGGTC TTCCTGGCCG GCCTGGCCAG GCAATCAATG GCAAGGATGG GGACCGAGGA 
TCCCCAGGGG CTCCAGGAGA GGCTGGGAGA CCTGGCCGGC CAGGCCCAGT GGGGCTACCA 
GGTTTCTGTG AGCCTGCCGC ATGCCTGGGG GCTTCAGCCT ATACCTCTGC TCGCCTCACA 
GAGCCTGGGT CCATCAAGGG GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interferon Regulatory Factor 6 Is Necessary for Salivary Glands and Pancreas Development.
Journal of dental research97(2)226-236(2018 Feb)
Metwalli KA,Do MA,Nguyen K,Mallick S,Kin K,Farokhnia N,Jun G,Fakhouri WD

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Early changes in morphology, bone mineral density and matrix composition of vertebrae lead to disc degeneration in aged collagen IX -/- mice.
Matrix biology : journal of the International Society for Matrix Biology49132-143(2016 Jan)
Kamper M,Hamann N,Prein C,Clausen-Schaumann H,Farkas Z,Aszodi A,Niehoff A,Paulsson M,Zaucke F

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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Col9a2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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