gRNAs
The following Col9a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col9a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu16841 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001290691.1 | |
| Accession Version | NM_001290691.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-16 | |
| Organism | Mus musculus(house mouse) | |
| Product | collagen alpha-1(IX) chain isoform 2 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY144829.1, AK160749.1, AK011547.2 and AK017395.1. On Mar 19, 2014 this sequence version replaced XM_006495648.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded protein (isoform 2) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK160749.1, BC038017.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCCTGGG CTGCCTGGGG ACGAGGAGTG CTTGGGCTGT CACTGATGCT GTCTGGGCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001277620.1 |
| CDS | 178..2220 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001290691.1 |
1 | ATGGCCTGGG CTGCCTGGGG ACGAGGAGTG CTTGGGCTGT CACTGATGCT GTCTGGGCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu27225 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006495649.3 | |
| Accession Version | XM_006495649.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | collagen alpha-1(IX) chain isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAGAACT TCTGGAAAAT CTCAGTTTTC TTCTGTGTGT GCAGTTGTCT GGGACCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006495712.1 |
| CDS | 267..1670 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006495649.3 |
1 | ATGAAGAACT TCTGGAAAAT CTCAGTTTTC TTCTGTGTGT GCAGTTGTCT GGGACCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu14289 | |
| Clone ID Related Accession (Same CDS sequence) | NM_007740.3 | |
| Accession Version | NM_007740.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-16 | |
| Organism | Mus musculus(house mouse) | |
| Product | collagen alpha-1(IX) chain isoform 1 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX207941.1, AK031071.1, L12215.1, AK004383.1 and AK017395.1. On Apr 14, 2009 this sequence version replaced NM_007740.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031071.1, D17511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGAAGAACT TCTGGAAAAT CTCAGTTTTC TTCTGTGTGT GCAGTTGTCT GGGACCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_031766.3 |
| CDS | 246..3011 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_007740.3 |
1 | ATGAAGAACT TCTGGAAAAT CTCAGTTTTC TTCTGTGTGT GCAGTTGTCT GGGACCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
BMP2 expression in the endocardial lineage is required for AV endocardial cushion maturation and remodeling. |
Absence of collagen IX accelerates hypertrophic differentiation in the embryonic mouse spine through a disturbance of the Ihh-PTHrP feedback loop. |
Early changes in morphology, bone mineral density and matrix composition of vertebrae lead to disc degeneration in aged collagen IX -/- mice. |
