Col9a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col9a1
Entrez Gene ID	12839
Full Name	collagen, type IX, alpha 1
Synonyms	Col9a-1
General protein information	Preferred Names collagen, type IX, alpha 1 Names collagen alpha-1(IX) chain alpha 1 type IX collagen procollagen, type IX, alpha 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 A5\|1 9.95 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007740.3	NP_031766.3	collagen alpha-1(IX) chain isoform 1 precursor
NM_001290691.1	NP_001277620.1	collagen alpha-1(IX) chain isoform 2 precursor
XM_006495649.3	XP_006495712.1	collagen alpha-1(IX) chain isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL9A1	XP_001136406.1
Canis lupus familiaris (dog)	COL9A1	XP_538994.3
Danio rerio (zebrafish)	col9a1	NP_998429.2
Rattus norvegicus (Norway rat)	Col9a1	NP_001094312.1
Mus musculus (house mouse)	Col9a1	NP_031766.3
Danio rerio (zebrafish)	zgc:194937	NP_001124096.1
	COL9A1	XP_001111640.1
Xenopus tropicalis (tropical clawed frog)	col9a1	XP_002939444.2
Homo sapiens (human)	COL9A1	NP_001842.3
Gallus gallus (chicken)	COL9A1	NP_001094381.1

Gene Ontology
Bibliography

Related articles in PubMed

BMP2 expression in the endocardial lineage is required for AV endocardial cushion maturation and remodeling.
Saxon JG, Baer DR, Barton JA, Hawkins T, Wu B, Trusk TC, Harris SE, Zhou B, Mishina Y, Sugi Y
Developmental biology430(1)113-128(2017 10)

Absence of collagen IX accelerates hypertrophic differentiation in the embryonic mouse spine through a disturbance of the Ihh-PTHrP feedback loop.
Kamper M, Paulsson M, Zaucke F
Cell and tissue research367(2)359-367(2017 Feb)

Early changes in morphology, bone mineral density and matrix composition of vertebrae lead to disc degeneration in aged collagen IX -/- mice.
Kamper M, Hamann N, Prein C, Clausen-Schaumann H, Farkas Z, Aszodi A, Niehoff A, Paulsson M, Zaucke F
Matrix biology : journal of the International Society for Matrix Biology49132-143(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An accelerated hypertrophic differentiation caused by a disturbed Ihh-PTHrP signaling pathway may lead to a higher bone mineral density in the vertebral bodies of newborn Col IX -/- mice and, as a result, to the early onset of disc degeneration.
Title: Absence of collagen IX accelerates hypertrophic differentiation in the embryonic mouse spine through a disturbance of the Ihh-PTHrP feedback loop.

Results show that absence of Col IX induces early developmental, structural and biomechanical alterations in both vertebral body and intervertebral disc which eventually cause severe degenerative changes in the aging spine.
Title: Early changes in morphology, bone mineral density and matrix composition of vertebrae lead to disc degeneration in aged collagen IX -/- mice.

Proteomic analysis of interaction partners of collagen alpha1 IX in the articular cartilage of mice.
Title: Comparative proteomic analysis of normal and collagen IX null mouse cartilage reveals altered extracellular matrix composition and novel components of the collagen IX interactome.

They test the hypothesis that aged Col9a1(-/-) mice would reduce joint pain by adopting locomotor behaviors that reduce both the magnitude and daily frequency of joint loading.
Title: Locomotor activity and gait in aged mice deficient for type IX collagen.

Loss of type IX collagen and COMP leads to matrix aberrations that may make cartilage more susceptible to degeneration.
Title: Combined role of type IX collagen and cartilage oligomeric matrix protein in cartilage matrix assembly: cartilage oligomeric matrix protein counteracts type IX collagen-induced limitation of cartilage collagen fibril growth in mouse chondrocyte cultures.

The following Col9a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col9a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16841	NM_001290691.1 Latest version!	Mus musculus collagen, type IX, alpha 1 (Col9a1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu27225	XM_006495649.3 Latest version!	Mus musculus collagen, type IX, alpha 1 (Col9a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu14289	NM_007740.3 Latest version!	Mus musculus collagen, type IX, alpha 1 (Col9a1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$545.30 $779.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col9a1 ( NM_001290691.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277620.1 Mus musculus collagen, type IX, alpha 1 (Col9a1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16841
Clone ID Related Accession (Same CDS sequence)	NM_001290691.1
Accession Version	NM_001290691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(IX) chain isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY144829.1, AK160749.1, AK011547.2 and AK017395.1. On Mar 19, 2014 this sequence version replaced XM_006495648.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded protein (isoform 2) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK160749.1, BC038017.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTGGG CTGCCTGGGG ACGAGGAGTG CTTGGGCTGT CACTGATGCT GTCTGGGCTC 
CGCTTGTGTG CTGCTCAAAG AGGTCCTCCG GGTGAGCAGG GGCCTCCAGG GCCTCCTGGG 
CCTCCTGGAG TTCCGGGCAT AGATGGCATT GATGGTGACC GAGGTCCAAA GGGTCCCCCA 
GGACCTCCGG GTCCTCCTGG AGACCCAGGC AAGCCAGGAG CACCAGGCAA GCCAGGCACA 
CCAGGAGCTG ATGGATTAAC AGGACCTGAT GGATCCCCTG GCTCTGTTGG ACCAAGGGGA 
CAAAAAGGAG AACCTGGTGT ACCTGGGTCT CGTGGATTTC CAGGCCGTGG TATTCCAGGA 
CCCCCTGGTC CTCCTGGGAC CACAGGACTT CCTGGAGAAC TTGGCCGAGT AGGCCCTATT 
GGAGACCCTG GGAAAAGAGG ACCACCTGGC CCTCCTGGAC CTCCAGGACC CAGTGGAACA 
ATTGGATTTC ATGATGGAGA CCCATTGTGC CCCAATTCCT GCCCACCAGG TCGCTCTGGA 
TATCCAGGCC TACCAGGCAT GAGGGGCCAT AAAGGGGCGA AAGGAGAAAT CGGCGAGCCA 
GGAAGACAAG GACACAAGGG TGAAGAGGGT GACCAGGGGG AACTGGGAGA AGTTGGCGAT 
CAAGGACCTC CAGGACCTCA GGGTCTGAGA GGCATCACTG GCATAGTTGG AGACAAAGGA 
GAAAAGGGTG CTCGGGGATT TGATGGAGAG CCTGGACCTC AGGGCATTCC AGGTGCAGCT 
GGTGATCAAG GACAGCGAGG CCCTCCAGGA GAAACAGGTC CTGAGGGAGA CAGAGGCATT 
CAAGGTTCCC GAGGAATTCC TGGATCCCCA GGGCCAAAAG GAGACACGGG CTTGCCAGGT 
GTAGATGGCC GAGATGGAAT ACCAGGAATG CCCGGGACAA AGGGTGAAGC AGGGAAGCCT 
GGACCCCCCG GGGACGTGGG ATTGCAGGGC TTACCAGGTG TCCCTGGAAT CCCTGGTGCA 
AAAGGTGTTG CCGGTGAAAA GGGTAACACG GGTGCTCCAG GAAAGCCCGG TCAGTTGGGA 
AGTTCAGGGA AACCAGGCCA ACAAGGGCCC CCAGGAGAGG TTGGACCTCG GGGACCCAGG 
GGCCTTCCAG GCAGTAGAGG CCCGGTAGGA CCAGAAGGGT CTCCAGGCAT ACCAGGGAAA 
CTGGGATCTG TTGGCAGCCC TGGCCTTCCT GGCTTGCCTG GGCCCCCTGG ACTTCCTGGA 
ATGAAAGGAG ACAGGGGTGT ATTTGGTGAA CCGGGTCCCA AGGGTGAACA GGGTGCCTCT 
GGTGAAGAAG GTGAAGCAGG AGCAAGGGGT GACCTTGGAG ATATGGGACA ACCTGGCCCA 
AAGGGATCTG TGGGTAACCC CGGGGAGCCG GGTCTGAGGG GGCCTGAAGG AATCAGAGGG 
CTTCCTGGAG TGGAAGGACC AAGAGGACCA CCTGGACCCC GGGGCATGCA GGGAGATCAG 
GGTGCCACTG GGCTGCCTGG TATCCAGGGC CCTCCGGGCA GAGCGCCGAC CGACCAGCAC 
ATCAAGCAGG TTTGCATGAG AGTCGTGCAA GAGCATTTTG CGGAAATGGC AGCCAGCCTC 
AAGAGACCAG ACACAGGAGC CTCTGGTCTT CCTGGGAGGC CTGGCCCCCC TGGGCCTCCG 
GGCCCCCCTG GAGAGAATGG TTTCCCTGGT CAGATGGGAA TCCGTGGTCT CCCAGGCATT 
AAGGGTCCCC CTGGTGCTCT TGGCTTAAGA GGACCTAAAG GAGACTTGGG AGAAAAAGGA 
GAACGTGGTC CTCCAGGAAG AGGTCCTAAG GGTTTGCCTG GAGCGATAGG TCTCCCAGGT 
GACCCAGGCC CTGCCAGCTA TGGGAAAAAT GGCCGTGACG GAGAGCAAGG TCCCCCGGGA 
GTGGCAGGAA TTCCTGGTGT GCCTGGACCC CCAGGTCCTC CAGGCCCTCC TGGGTTCTGT 
GAGCCAGCCT CTTGCACCCT GCAGTCTGGT CAAAGAGCAT TTAGCAAAGG GCCGGACAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277620.1
CDS	178..2220
Translation	MAWAAWGRGVLGLSLMLSGLRLCAAQRGPPGEQGPPGPPGPPGVPGIDGIDGDRGPKGPPGPPGPPGDPGKPGAPGKPGTPGADGLTGPDGSPGSVGPRGQKGEPGVPGSRGFPGRGIPGPPGPPGTTGLPGELGRVGPIGDPGKRGPPGPPGPPGPSGTIGFHDGDPLCPNSCPPGRSGYPGLPGMRGHKGAKGEIGEPGRQGHKGEEGDQGELGEVGDQGPPGPQGLRGITGIVGDKGEKGARGFDGEPGPQGIPGAAGDQGQRGPPGETGPEGDRGIQGSRGIPGSPGPKGDTGLPGVDGRDGIPGMPGTKGEAGKPGPPGDVGLQGLPGVPGIPGAKGVAGEKGNTGAPGKPGQLGSSGKPGQQGPPGEVGPRGPRGLPGSRGPVGPEGSPGIPGKLGSVGSPGLPGLPGPPGLPGMKGDRGVFGEPGPKGEQGASGEEGEAGARGDLGDMGQPGPKGSVGNPGEPGLRGPEGIRGLPGVEGPRGPPGPRGMQGDQGATGLPGIQGPPGRAPTDQHIKQVCMRVVQEHFAEMAASLKRPDTGASGLPGRPGPPGPPGPPGENGFPGQMGIRGLPGIKGPPGALGLRGPKGDLGEKGERGPPGRGPKGLPGAIGLPGDPGPASYGKNGRDGEQGPPGVAGIPGVPGPPGPPGPPGFCEPASCTLQSGQRAFSKGPDK

Target ORF information:

RefSeq Version	NM_001290691.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IX, alpha 1 (Col9a1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290691.1

ORF Insert Sequence:

ATGGCCTGGG CTGCCTGGGG ACGAGGAGTG CTTGGGCTGT CACTGATGCT GTCTGGGCTC 
CGCTTGTGTG CTGCTCAAAG AGGTCCTCCG GGTGAGCAGG GGCCTCCAGG GCCTCCTGGG 
CCTCCTGGAG TTCCGGGCAT AGATGGCATT GATGGTGACC GAGGTCCAAA GGGTCCCCCA 
GGACCTCCGG GTCCTCCTGG AGACCCAGGC AAGCCAGGAG CACCAGGCAA GCCAGGCACA 
CCAGGAGCTG ATGGATTAAC AGGACCTGAT GGATCCCCTG GCTCTGTTGG ACCAAGGGGA 
CAAAAAGGAG AACCTGGTGT ACCTGGGTCT CGTGGATTTC CAGGCCGTGG TATTCCAGGA 
CCCCCTGGTC CTCCTGGGAC CACAGGACTT CCTGGAGAAC TTGGCCGAGT AGGCCCTATT 
GGAGACCCTG GGAAAAGAGG ACCACCTGGC CCTCCTGGAC CTCCAGGACC CAGTGGAACA 
ATTGGATTTC ATGATGGAGA CCCATTGTGC CCCAATTCCT GCCCACCAGG TCGCTCTGGA 
TATCCAGGCC TACCAGGCAT GAGGGGCCAT AAAGGGGCGA AAGGAGAAAT CGGCGAGCCA 
GGAAGACAAG GACACAAGGG TGAAGAGGGT GACCAGGGGG AACTGGGAGA AGTTGGCGAT 
CAAGGACCTC CAGGACCTCA GGGTCTGAGA GGCATCACTG GCATAGTTGG AGACAAAGGA 
GAAAAGGGTG CTCGGGGATT TGATGGAGAG CCTGGACCTC AGGGCATTCC AGGTGCAGCT 
GGTGATCAAG GACAGCGAGG CCCTCCAGGA GAAACAGGTC CTGAGGGAGA CAGAGGCATT 
CAAGGTTCCC GAGGAATTCC TGGATCCCCA GGGCCAAAAG GAGACACGGG CTTGCCAGGT 
GTAGATGGCC GAGATGGAAT ACCAGGAATG CCCGGGACAA AGGGTGAAGC AGGGAAGCCT 
GGACCCCCCG GGGACGTGGG ATTGCAGGGC TTACCAGGTG TCCCTGGAAT CCCTGGTGCA 
AAAGGTGTTG CCGGTGAAAA GGGTAACACG GGTGCTCCAG GAAAGCCCGG TCAGTTGGGA 
AGTTCAGGGA AACCAGGCCA ACAAGGGCCC CCAGGAGAGG TTGGACCTCG GGGACCCAGG 
GGCCTTCCAG GCAGTAGAGG CCCGGTAGGA CCAGAAGGGT CTCCAGGCAT ACCAGGGAAA 
CTGGGATCTG TTGGCAGCCC TGGCCTTCCT GGCTTGCCTG GGCCCCCTGG ACTTCCTGGA 
ATGAAAGGAG ACAGGGGTGT ATTTGGTGAA CCGGGTCCCA AGGGTGAACA GGGTGCCTCT 
GGTGAAGAAG GTGAAGCAGG AGCAAGGGGT GACCTTGGAG ATATGGGACA ACCTGGCCCA 
AAGGGATCTG TGGGTAACCC CGGGGAGCCG GGTCTGAGGG GGCCTGAAGG AATCAGAGGG 
CTTCCTGGAG TGGAAGGACC AAGAGGACCA CCTGGACCCC GGGGCATGCA GGGAGATCAG 
GGTGCCACTG GGCTGCCTGG TATCCAGGGC CCTCCGGGCA GAGCGCCGAC CGACCAGCAC 
ATCAAGCAGG TTTGCATGAG AGTCGTGCAA GAGCATTTTG CGGAAATGGC AGCCAGCCTC 
AAGAGACCAG ACACAGGAGC CTCTGGTCTT CCTGGGAGGC CTGGCCCCCC TGGGCCTCCG 
GGCCCCCCTG GAGAGAATGG TTTCCCTGGT CAGATGGGAA TCCGTGGTCT CCCAGGCATT 
AAGGGTCCCC CTGGTGCTCT TGGCTTAAGA GGACCTAAAG GAGACTTGGG AGAAAAAGGA 
GAACGTGGTC CTCCAGGAAG AGGTCCTAAG GGTTTGCCTG GAGCGATAGG TCTCCCAGGT 
GACCCAGGCC CTGCCAGCTA TGGGAAAAAT GGCCGTGACG GAGAGCAAGG TCCCCCGGGA 
GTGGCAGGAA TTCCTGGTGT GCCTGGACCC CCAGGTCCTC CAGGCCCTCC TGGGTTCTGT 
GAGCCAGCCT CTTGCACCCT GCAGTCTGGT CAAAGAGCAT TTAGCAAAGG GCCGGACAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col9a1 ( XM_006495649.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495712.1 Mus musculus collagen, type IX, alpha 1 (Col9a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27225
Clone ID Related Accession (Same CDS sequence)	XM_006495649.3
Accession Version	XM_006495649.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(IX) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACT TCTGGAAAAT CTCAGTTTTC TTCTGTGTGT GCAGTTGTCT GGGACCCTGG 
GTATCCGCAA CTCTTAAGCG TCGTGCAAGA TTTCCTGCCA ATTCCATTTC TAATGGTGGA 
AGTGAACTGT GTCCCAAGAT CAGGATTGGC CAAGATGACT TACCAGGGTT TGACCTGATT 
TCTCAGTTCC AAATTGAGAA GGCTGCATCT CGAAGGACTA TCCAGAGGGT GGTGGGATCC 
ACAGCCTTAC AAGTGGCTTA CAAGCTGGGA AGTAATGTAG ACTTCAGGAT TCCAACAAGG 
CATTTGTATC CCAGTGGACT GCCTGAAGAA TATTCCTTTT TAACTACTTT CCGGATGACT 
GGAAGCACAC TTGAAAAGCA CTGGAACATT TGGCAGATCC AGGATTCCGC AGGGAGAGAG 
CAAGTTGGCG TGAAGATAAA TGGCCAAACA AAGTCTGTCG CATTTTCATA CAAGGGACTG 
GATGGGAGTC TCCAAACGGC TGCCTTCTTG AATTTGCCGT CCTTGTTTGA CTCCCGGTGG 
CATAAGCTCA TGATTGGCGT GGAAAGAACA AGCGCCACTC TTTTTATTGA CTGCATCAGG 
ATCGAATCTT TACCTATAAA GCCAAGAGGC CAGATTGATG CGGATGGCTT TGCAGTGCTG 
GGAAAACTTG TGGACAATCC TCAGGTTTCT GTTCCTTTTG AACTCCAGTG GATGCTGATT 
CATTGTGACC CCCTGAGACC CAGGAGAGAA ACCTGTCATG AGCTGCCAAT CAGAATCACA 
ACCAGCCAGA CCACTGATGA GAGAGGTCCT CCGGGTGAGC AGGGGCCTCC AGGGCCTCCT 
GGGCCTCCTG GAGTTCCGGG CATAGATGGC ATTGATGGTG ACCGAGGTCC AAAGGGTCCC 
CCAGGACCTC CGGGTCCTCC TGGAGACCCA GGCAAGCCAG GAGCACCAGG CAAGCCAGGC 
ACACCAGGAG CTGATGGATT AACAGGACCT GATGGATCCC CTGGCTCTGT TGGACCAAGG 
GGACAAAAAG GAGAACCTGG TGTACCTGGG TCTCGTGGAT TTCCAGGCCG TGGTATTCCA 
GGACCCCCTG GTCCTCCTGG GACCACAGGA CTTCCTGGAG AACTTGGCCG AGTAGGCCCT 
ATTGGAGACC CTGGGAAAAG AGGACCACCT GGCCCTCCTG GACCTCCAGG ACCCAGTGGA 
ACAATTGGAT TTCATGATGG AGACCCATTG TGCCCCAATT CCTGCCCACC AGGTCGCTCT 
GGATATCCAG GCCTACCAGG CATGAGGGGC CATAAAGGGG CGAAAGGAGA AATCGGCGAG 
CCAGGAAGAC AAGGACACAA GGTAAGGAAA GGGCATTTAG TAGGTCACTG TGACTATAGT 
GATGCATTTA GTAGGTCACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495712.1
CDS	267..1670
Translation	MKNFWKISVFFCVCSCLGPWVSATLKRRARFPANSISNGGSELCPKIRIGQDDLPGFDLISQFQIEKAASRRTIQRVVGSTALQVAYKLGSNVDFRIPTRHLYPSGLPEEYSFLTTFRMTGSTLEKHWNIWQIQDSAGREQVGVKINGQTKSVAFSYKGLDGSLQTAAFLNLPSLFDSRWHKLMIGVERTSATLFIDCIRIESLPIKPRGQIDADGFAVLGKLVDNPQVSVPFELQWMLIHCDPLRPRRETCHELPIRITTSQTTDERGPPGEQGPPGPPGPPGVPGIDGIDGDRGPKGPPGPPGPPGDPGKPGAPGKPGTPGADGLTGPDGSPGSVGPRGQKGEPGVPGSRGFPGRGIPGPPGPPGTTGLPGELGRVGPIGDPGKRGPPGPPGPPGPSGTIGFHDGDPLCPNSCPPGRSGYPGLPGMRGHKGAKGEIGEPGRQGHKVRKGHLVGHCDYSDAFSRSL

Target ORF information:

RefSeq Version	XM_006495649.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IX, alpha 1 (Col9a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495649.3

ORF Insert Sequence:

ATGAAGAACT TCTGGAAAAT CTCAGTTTTC TTCTGTGTGT GCAGTTGTCT GGGACCCTGG 
GTATCCGCAA CTCTTAAGCG TCGTGCAAGA TTTCCTGCCA ATTCCATTTC TAATGGTGGA 
AGTGAACTGT GTCCCAAGAT CAGGATTGGC CAAGATGACT TACCAGGGTT TGACCTGATT 
TCTCAGTTCC AAATTGAGAA GGCTGCATCT CGAAGGACTA TCCAGAGGGT GGTGGGATCC 
ACAGCCTTAC AAGTGGCTTA CAAGCTGGGA AGTAATGTAG ACTTCAGGAT TCCAACAAGG 
CATTTGTATC CCAGTGGACT GCCTGAAGAA TATTCCTTTT TAACTACTTT CCGGATGACT 
GGAAGCACAC TTGAAAAGCA CTGGAACATT TGGCAGATCC AGGATTCCGC AGGGAGAGAG 
CAAGTTGGCG TGAAGATAAA TGGCCAAACA AAGTCTGTCG CATTTTCATA CAAGGGACTG 
GATGGGAGTC TCCAAACGGC TGCCTTCTTG AATTTGCCGT CCTTGTTTGA CTCCCGGTGG 
CATAAGCTCA TGATTGGCGT GGAAAGAACA AGCGCCACTC TTTTTATTGA CTGCATCAGG 
ATCGAATCTT TACCTATAAA GCCAAGAGGC CAGATTGATG CGGATGGCTT TGCAGTGCTG 
GGAAAACTTG TGGACAATCC TCAGGTTTCT GTTCCTTTTG AACTCCAGTG GATGCTGATT 
CATTGTGACC CCCTGAGACC CAGGAGAGAA ACCTGTCATG AGCTGCCAAT CAGAATCACA 
ACCAGCCAGA CCACTGATGA GAGAGGTCCT CCGGGTGAGC AGGGGCCTCC AGGGCCTCCT 
GGGCCTCCTG GAGTTCCGGG CATAGATGGC ATTGATGGTG ACCGAGGTCC AAAGGGTCCC 
CCAGGACCTC CGGGTCCTCC TGGAGACCCA GGCAAGCCAG GAGCACCAGG CAAGCCAGGC 
ACACCAGGAG CTGATGGATT AACAGGACCT GATGGATCCC CTGGCTCTGT TGGACCAAGG 
GGACAAAAAG GAGAACCTGG TGTACCTGGG TCTCGTGGAT TTCCAGGCCG TGGTATTCCA 
GGACCCCCTG GTCCTCCTGG GACCACAGGA CTTCCTGGAG AACTTGGCCG AGTAGGCCCT 
ATTGGAGACC CTGGGAAAAG AGGACCACCT GGCCCTCCTG GACCTCCAGG ACCCAGTGGA 
ACAATTGGAT TTCATGATGG AGACCCATTG TGCCCCAATT CCTGCCCACC AGGTCGCTCT 
GGATATCCAG GCCTACCAGG CATGAGGGGC CATAAAGGGG CGAAAGGAGA AATCGGCGAG 
CCAGGAAGAC AAGGACACAA GGTAAGGAAA GGGCATTTAG TAGGTCACTG TGACTATAGT 
GATGCATTTA GTAGGTCACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col9a1 ( NM_007740.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031766.3 Mus musculus collagen, type IX, alpha 1 (Col9a1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14289
Clone ID Related Accession (Same CDS sequence)	NM_007740.3
Accession Version	NM_007740.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(IX) chain isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX207941.1, AK031071.1, L12215.1, AK004383.1 and AK017395.1. On Apr 14, 2009 this sequence version replaced NM_007740.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031071.1, D17511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGAAGAACT TCTGGAAAAT CTCAGTTTTC TTCTGTGTGT GCAGTTGTCT GGGACCCTGG 
GTATCCGCAA CTCTTAAGCG TCGTGCAAGA TTTCCTGCCA ATTCCATTTC TAATGGTGGA 
AGTGAACTGT GTCCCAAGAT CAGGATTGGC CAAGATGACT TACCAGGGTT TGACCTGATT 
TCTCAGTTCC AAATTGAGAA GGCTGCATCT CGAAGGACTA TCCAGAGGGT GGTGGGATCC 
ACAGCCTTAC AAGTGGCTTA CAAGCTGGGA AGTAATGTAG ACTTCAGGAT TCCAACAAGG 
CATTTGTATC CCAGTGGACT GCCTGAAGAA TATTCCTTTT TAACTACTTT CCGGATGACT 
GGAAGCACAC TTGAAAAGCA CTGGAACATT TGGCAGATCC AGGATTCCGC AGGGAGAGAG 
CAAGTTGGCG TGAAGATAAA TGGCCAAACA AAGTCTGTCG CATTTTCATA CAAGGGACTG 
GATGGGAGTC TCCAAACGGC TGCCTTCTTG AATTTGCCGT CCTTGTTTGA CTCCCGGTGG 
CATAAGCTCA TGATTGGCGT GGAAAGAACA AGCGCCACTC TTTTTATTGA CTGCATCAGG 
ATCGAATCTT TACCTATAAA GCCAAGAGGC CAGATTGATG CGGATGGCTT TGCAGTGCTG 
GGAAAACTTG TGGACAATCC TCAGGTTTCT GTTCCTTTTG AACTCCAGTG GATGCTGATT 
CATTGTGACC CCCTGAGACC CAGGAGAGAA ACCTGTCATG AGCTGCCAAT CAGAATCACA 
ACCAGCCAGA CCACTGATGA GAGAGGTCCT CCGGGTGAGC AGGGGCCTCC AGGGCCTCCT 
GGGCCTCCTG GAGTTCCGGG CATAGATGGC ATTGATGGTG ACCGAGGTCC AAAGGGTCCC 
CCAGGACCTC CGGGTCCTCC TGGAGACCCA GGCAAGCCAG GAGCACCAGG CAAGCCAGGC 
ACACCAGGAG CTGATGGATT AACAGGACCT GATGGATCCC CTGGCTCTGT TGGACCAAGG 
GGACAAAAAG GAGAACCTGG TGTACCTGGG TCTCGTGGAT TTCCAGGCCG TGGTATTCCA 
GGACCCCCTG GTCCTCCTGG GACCACAGGA CTTCCTGGAG AACTTGGCCG AGTAGGCCCT 
ATTGGAGACC CTGGGAAAAG AGGACCACCT GGCCCTCCTG GACCTCCAGG ACCCAGTGGA 
ACAATTGGAT TTCATGATGG AGACCCATTG TGCCCCAATT CCTGCCCACC AGGTCGCTCT 
GGATATCCAG GCCTACCAGG CATGAGGGGC CATAAAGGGG CGAAAGGAGA AATCGGCGAG 
CCAGGAAGAC AAGGACACAA GGGTGAAGAG GGTGACCAGG GGGAACTGGG AGAAGTTGGC 
GATCAAGGAC CTCCAGGACC TCAGGGTCTG AGAGGCATCA CTGGCATAGT TGGAGACAAA 
GGAGAAAAGG GTGCTCGGGG ATTTGATGGA GAGCCTGGAC CTCAGGGCAT TCCAGGTGCA 
GCTGGTGATC AAGGACAGCG AGGCCCTCCA GGAGAAACAG GTCCTGAGGG AGACAGAGGC 
ATTCAAGGTT CCCGAGGAAT TCCTGGATCC CCAGGGCCAA AAGGAGACAC GGGCTTGCCA 
GGTGTAGATG GCCGAGATGG AATACCAGGA ATGCCCGGGA CAAAGGGTGA AGCAGGGAAG 
CCTGGACCCC CCGGGGACGT GGGATTGCAG GGCTTACCAG GTGTCCCTGG AATCCCTGGT 
GCAAAAGGTG TTGCCGGTGA AAAGGGTAAC ACGGGTGCTC CAGGAAAGCC CGGTCAGTTG 
GGAAGTTCAG GGAAACCAGG CCAACAAGGG CCCCCAGGAG AGGTTGGACC TCGGGGACCC 
AGGGGCCTTC CAGGCAGTAG AGGCCCGGTA GGACCAGAAG GGTCTCCAGG CATACCAGGG 
AAACTGGGAT CTGTTGGCAG CCCTGGCCTT CCTGGCTTGC CTGGGCCCCC TGGACTTCCT 
GGAATGAAAG GAGACAGGGG TGTATTTGGT GAACCGGGTC CCAAGGGTGA ACAGGGTGCC 
TCTGGTGAAG AAGGTGAAGC AGGAGCAAGG GGTGACCTTG GAGATATGGG ACAACCTGGC 
CCAAAGGGAT CTGTGGGTAA CCCCGGGGAG CCGGGTCTGA GGGGGCCTGA AGGAATCAGA 
GGGCTTCCTG GAGTGGAAGG ACCAAGAGGA CCACCTGGAC CCCGGGGCAT GCAGGGAGAT 
CAGGGTGCCA CTGGGCTGCC TGGTATCCAG GGCCCTCCGG GCAGAGCGCC GACCGACCAG 
CACATCAAGC AGGTTTGCAT GAGAGTCGTG CAAGAGCATT TTGCGGAAAT GGCAGCCAGC 
CTCAAGAGAC CAGACACAGG AGCCTCTGGT CTTCCTGGGA GGCCTGGCCC CCCTGGGCCT 
CCGGGCCCCC CTGGAGAGAA TGGTTTCCCT GGTCAGATGG GAATCCGTGG TCTCCCAGGC 
ATTAAGGGTC CCCCTGGTGC TCTTGGCTTA AGAGGACCTA AAGGAGACTT GGGAGAAAAA 
GGAGAACGTG GTCCTCCAGG AAGAGGTCCT AAGGGTTTGC CTGGAGCGAT AGGTCTCCCA 
GGTGACCCAG GCCCTGCCAG CTATGGGAAA AATGGCCGTG ACGGAGAGCA AGGTCCCCCG 
GGAGTGGCAG GAATTCCTGG TGTGCCTGGA CCCCCAGGTC CTCCAGGCCC TCCTGGGTTC 
TGTGAGCCAG CCTCTTGCAC CCTGCAGTCT GGTCAAAGAG CATTTAGCAA AGGGCCGGAC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031766.3
CDS	246..3011
Translation	MKNFWKISVFFCVCSCLGPWVSATLKRRARFPANSISNGGSELCPKIRIGQDDLPGFDLISQFQIEKAASRRTIQRVVGSTALQVAYKLGSNVDFRIPTRHLYPSGLPEEYSFLTTFRMTGSTLEKHWNIWQIQDSAGREQVGVKINGQTKSVAFSYKGLDGSLQTAAFLNLPSLFDSRWHKLMIGVERTSATLFIDCIRIESLPIKPRGQIDADGFAVLGKLVDNPQVSVPFELQWMLIHCDPLRPRRETCHELPIRITTSQTTDERGPPGEQGPPGPPGPPGVPGIDGIDGDRGPKGPPGPPGPPGDPGKPGAPGKPGTPGADGLTGPDGSPGSVGPRGQKGEPGVPGSRGFPGRGIPGPPGPPGTTGLPGELGRVGPIGDPGKRGPPGPPGPPGPSGTIGFHDGDPLCPNSCPPGRSGYPGLPGMRGHKGAKGEIGEPGRQGHKGEEGDQGELGEVGDQGPPGPQGLRGITGIVGDKGEKGARGFDGEPGPQGIPGAAGDQGQRGPPGETGPEGDRGIQGSRGIPGSPGPKGDTGLPGVDGRDGIPGMPGTKGEAGKPGPPGDVGLQGLPGVPGIPGAKGVAGEKGNTGAPGKPGQLGSSGKPGQQGPPGEVGPRGPRGLPGSRGPVGPEGSPGIPGKLGSVGSPGLPGLPGPPGLPGMKGDRGVFGEPGPKGEQGASGEEGEAGARGDLGDMGQPGPKGSVGNPGEPGLRGPEGIRGLPGVEGPRGPPGPRGMQGDQGATGLPGIQGPPGRAPTDQHIKQVCMRVVQEHFAEMAASLKRPDTGASGLPGRPGPPGPPGPPGENGFPGQMGIRGLPGIKGPPGALGLRGPKGDLGEKGERGPPGRGPKGLPGAIGLPGDPGPASYGKNGRDGEQGPPGVAGIPGVPGPPGPPGPPGFCEPASCTLQSGQRAFSKGPDK

Target ORF information:

RefSeq Version	NM_007740.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IX, alpha 1 (Col9a1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007740.3

ORF Insert Sequence:

ATGAAGAACT TCTGGAAAAT CTCAGTTTTC TTCTGTGTGT GCAGTTGTCT GGGACCCTGG 
GTATCCGCAA CTCTTAAGCG TCGTGCAAGA TTTCCTGCCA ATTCCATTTC TAATGGTGGA 
AGTGAACTGT GTCCCAAGAT CAGGATTGGC CAAGATGACT TACCAGGGTT TGACCTGATT 
TCTCAGTTCC AAATTGAGAA GGCTGCATCT CGAAGGACTA TCCAGAGGGT GGTGGGATCC 
ACAGCCTTAC AAGTGGCTTA CAAGCTGGGA AGTAATGTAG ACTTCAGGAT TCCAACAAGG 
CATTTGTATC CCAGTGGACT GCCTGAAGAA TATTCCTTTT TAACTACTTT CCGGATGACT 
GGAAGCACAC TTGAAAAGCA CTGGAACATT TGGCAGATCC AGGATTCCGC AGGGAGAGAG 
CAAGTTGGCG TGAAGATAAA TGGCCAAACA AAGTCTGTCG CATTTTCATA CAAGGGACTG 
GATGGGAGTC TCCAAACGGC TGCCTTCTTG AATTTGCCGT CCTTGTTTGA CTCCCGGTGG 
CATAAGCTCA TGATTGGCGT GGAAAGAACA AGCGCCACTC TTTTTATTGA CTGCATCAGG 
ATCGAATCTT TACCTATAAA GCCAAGAGGC CAGATTGATG CGGATGGCTT TGCAGTGCTG 
GGAAAACTTG TGGACAATCC TCAGGTTTCT GTTCCTTTTG AACTCCAGTG GATGCTGATT 
CATTGTGACC CCCTGAGACC CAGGAGAGAA ACCTGTCATG AGCTGCCAAT CAGAATCACA 
ACCAGCCAGA CCACTGATGA GAGAGGTCCT CCGGGTGAGC AGGGGCCTCC AGGGCCTCCT 
GGGCCTCCTG GAGTTCCGGG CATAGATGGC ATTGATGGTG ACCGAGGTCC AAAGGGTCCC 
CCAGGACCTC CGGGTCCTCC TGGAGACCCA GGCAAGCCAG GAGCACCAGG CAAGCCAGGC 
ACACCAGGAG CTGATGGATT AACAGGACCT GATGGATCCC CTGGCTCTGT TGGACCAAGG 
GGACAAAAAG GAGAACCTGG TGTACCTGGG TCTCGTGGAT TTCCAGGCCG TGGTATTCCA 
GGACCCCCTG GTCCTCCTGG GACCACAGGA CTTCCTGGAG AACTTGGCCG AGTAGGCCCT 
ATTGGAGACC CTGGGAAAAG AGGACCACCT GGCCCTCCTG GACCTCCAGG ACCCAGTGGA 
ACAATTGGAT TTCATGATGG AGACCCATTG TGCCCCAATT CCTGCCCACC AGGTCGCTCT 
GGATATCCAG GCCTACCAGG CATGAGGGGC CATAAAGGGG CGAAAGGAGA AATCGGCGAG 
CCAGGAAGAC AAGGACACAA GGGTGAAGAG GGTGACCAGG GGGAACTGGG AGAAGTTGGC 
GATCAAGGAC CTCCAGGACC TCAGGGTCTG AGAGGCATCA CTGGCATAGT TGGAGACAAA 
GGAGAAAAGG GTGCTCGGGG ATTTGATGGA GAGCCTGGAC CTCAGGGCAT TCCAGGTGCA 
GCTGGTGATC AAGGACAGCG AGGCCCTCCA GGAGAAACAG GTCCTGAGGG AGACAGAGGC 
ATTCAAGGTT CCCGAGGAAT TCCTGGATCC CCAGGGCCAA AAGGAGACAC GGGCTTGCCA 
GGTGTAGATG GCCGAGATGG AATACCAGGA ATGCCCGGGA CAAAGGGTGA AGCAGGGAAG 
CCTGGACCCC CCGGGGACGT GGGATTGCAG GGCTTACCAG GTGTCCCTGG AATCCCTGGT 
GCAAAAGGTG TTGCCGGTGA AAAGGGTAAC ACGGGTGCTC CAGGAAAGCC CGGTCAGTTG 
GGAAGTTCAG GGAAACCAGG CCAACAAGGG CCCCCAGGAG AGGTTGGACC TCGGGGACCC 
AGGGGCCTTC CAGGCAGTAG AGGCCCGGTA GGACCAGAAG GGTCTCCAGG CATACCAGGG 
AAACTGGGAT CTGTTGGCAG CCCTGGCCTT CCTGGCTTGC CTGGGCCCCC TGGACTTCCT 
GGAATGAAAG GAGACAGGGG TGTATTTGGT GAACCGGGTC CCAAGGGTGA ACAGGGTGCC 
TCTGGTGAAG AAGGTGAAGC AGGAGCAAGG GGTGACCTTG GAGATATGGG ACAACCTGGC 
CCAAAGGGAT CTGTGGGTAA CCCCGGGGAG CCGGGTCTGA GGGGGCCTGA AGGAATCAGA 
GGGCTTCCTG GAGTGGAAGG ACCAAGAGGA CCACCTGGAC CCCGGGGCAT GCAGGGAGAT 
CAGGGTGCCA CTGGGCTGCC TGGTATCCAG GGCCCTCCGG GCAGAGCGCC GACCGACCAG 
CACATCAAGC AGGTTTGCAT GAGAGTCGTG CAAGAGCATT TTGCGGAAAT GGCAGCCAGC 
CTCAAGAGAC CAGACACAGG AGCCTCTGGT CTTCCTGGGA GGCCTGGCCC CCCTGGGCCT 
CCGGGCCCCC CTGGAGAGAA TGGTTTCCCT GGTCAGATGG GAATCCGTGG TCTCCCAGGC 
ATTAAGGGTC CCCCTGGTGC TCTTGGCTTA AGAGGACCTA AAGGAGACTT GGGAGAAAAA 
GGAGAACGTG GTCCTCCAGG AAGAGGTCCT AAGGGTTTGC CTGGAGCGAT AGGTCTCCCA 
GGTGACCCAG GCCCTGCCAG CTATGGGAAA AATGGCCGTG ACGGAGAGCA AGGTCCCCCG 
GGAGTGGCAG GAATTCCTGG TGTGCCTGGA CCCCCAGGTC CTCCAGGCCC TCCTGGGTTC 
TGTGAGCCAG CCTCTTGCAC CCTGCAGTCT GGTCAAAGAG CATTTAGCAA AGGGCCGGAC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BMP2 expression in the endocardial lineage is required for AV endocardial cushion maturation and remodeling.
Developmental biology430(1)113-128(2017 10)
Saxon JG,Baer DR,Barton JA,Hawkins T,Wu B,Trusk TC,Harris SE,Zhou B,Mishina Y,Sugi Y

Absence of collagen IX accelerates hypertrophic differentiation in the embryonic mouse spine through a disturbance of the Ihh-PTHrP feedback loop.
Cell and tissue research367(2)359-367(2017 Feb)
Kamper M,Paulsson M,Zaucke F

Early changes in morphology, bone mineral density and matrix composition of vertebrae lead to disc degeneration in aged collagen IX -/- mice.
Matrix biology : journal of the International Society for Matrix Biology49132-143(2016 Jan)
Kamper M,Hamann N,Prein C,Clausen-Schaumann H,Farkas Z,Aszodi A,Niehoff A,Paulsson M,Zaucke F

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Col9a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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