Col5a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col5a1
Entrez Gene ID	12831
Full Name	collagen, type V, alpha 1
Synonyms	AI413331
General protein information	Preferred Names collagen, type V, alpha 1 Names collagen alpha-1(V) chain alpha1(V) collagen pro-alpha1(V) collagen procollagen type V alpha 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 A3\|2 19.38 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_015734.2	NP_056549.2	collagen alpha-1(V) chain precursor
XM_006497644.3	XP_006497707.1	collagen alpha-1(V) chain isoform X1
XM_006497644.4	XP_006497707.1	collagen alpha-1(V) chain isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col5a1	NP_604447.1
Homo sapiens (human)	COL5A1	NP_001265003.1
Mus musculus (house mouse)	Col5a1	NP_056549.2
Gallus gallus (chicken)	COL5A1	NP_990121.1
Xenopus tropicalis (tropical clawed frog)	col5a1	XP_004916702.1
Danio rerio (zebrafish)	col5a1	XP_002665924.2

Gene Ontology
Bibliography

Related articles in PubMed

KLF13 is a genetic modifier of the Holt-Oram syndrome gene TBX5.
Darwich R, Li W, Yamak A, Komati H, Andelfinger G, Sun K, Nemer M
Human molecular genetics26(5)942-954(2017 03)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Significantly decreased fibroblast proliferation was observed in Col5a1(+/-) compared to Col5a1(+/+) fibroblasts.
Title: Altered dermal fibroblast behavior in a collagen V haploinsufficient murine model of classic Ehlers-Danlos syndrome.

Col5a1 knockout stroma demonstrated severe dysfunctional regulation of fibrillogenesis.
Title: Collagen V is a dominant regulator of collagen fibrillogenesis: dysfunctional regulation of structure and function in a corneal-stroma-specific Col5a1-null mouse model.

Col(V) is an established autoantigen in atherosclerotic mice, and Col(V) autoimmunity is a consistent feature, with variable T helper type (Th)1 cell pathway involvement.
Title: Interleukin-17-dependent autoimmunity to collagen type V in atherosclerosis.

Sp7/Osterix was observed to participate in the activation of the Col5a1 promoter during osteoblast differentiation.
Title: Sp7/Osterix is involved in the up-regulation of the mouse pro-?1(V) collagen gene (Col5a1) in osteoblastic cells.

This study shows the presence of collagen types I, III, and V in the endometrial stroma of implantation and interimplantation sites from days 5 to 8 of pregnancy in the mouse.
Title: Distribution of collagen types I, III, and V in pregnant mouse endometrium.

The following Col5a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col5a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu29274	XM_006497644.4 Latest version!	Mus musculus collagen, type V, alpha 1 (Col5a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu21268	NM_015734.2 Latest version!	Mus musculus collagen, type V, alpha 1 (Col5a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1350.30 $1929.00
View Old Accession Versions >>
OMu29274	XM_006497644.3	Mus musculus collagen, type V, alpha 1 (Col5a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col5a1 ( XM_006497644.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497707.1 Mus musculus collagen, type V, alpha 1 (Col5a1), transcript variant X1, mRNA.

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CloneID	OMu29274
Clone ID Related Accession (Same CDS sequence)	XM_006497644.3 , XM_006497644.4
Accession Version	XM_006497644.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497644.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTAC ACACCCGCTG GAAAGCTGCG CGCCCAGGCG CCCTGCTGCT GTCTTCGCCG 
CTACTCCTGT TCCTGCTGCT GCTGTGGGCG CCGCCTTCGA GCCGCGCAGC TCAGCCAGCA 
GATCTTCTGG AGATGCTAGA TTTTCACAAT TTGCCCTCAG GGGTAACGAA AACCACAGGT 
TTCTGTGCTA CTCGAAGATC TTCCAGCGAG CCGGATGTTG CCTACCGAGT CTCTAAAGAT 
GCACAGCTCA GCATGCCCAC CAAGCAGCTG TACCCTGAGT CTGGTTTTCC CGAGGACTTC 
TCCATCCTGA CAACCGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTAGT CTCCATTTAC 
AATGAGCAGG GCATCCAGCA GTTGGGGCTG GAGCTGGGCC GCTCCCCTGT CTTCCTCTAT 
GAGGACCACA CAGGGAAGCC CGGGCCTGAA GAGTATCCGC TTTTCCCTGG CATCAACTTG 
TCCGATGGCA AGTGGCACCG AATTGCTCTC AGTGTCTACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AGAAGAAGAT TACGAAGTTC CTCAGCCGCA GTGACCACCC CATAATAGAC 
ACCAATGGGA TTGTCATGTT TGGCTCCCGG ATTCTGGATG ATGAAATATT TGAGGGTGAC 
ATCCAACAGT TGCTTTTCGT CTCTGACAAC CGAGCTGCCT ATGACTACTG TGAGCACTAC 
AGCCCCGACT GTGACACTGC GGTCCCTGAC ACACCTCAGT CACAGGACCC TAACCCGGAT 
GAATATTACC CAGAAGGAGA GGGTGAGACC TATTACTATG AGTATCCATA TTATGAAGAC 
CCTGAAGACC CGGGAAAGGA GCCTGCCCCT ACTCAGAAGC CAGTGGAAGC TGCCAGAGAA 
ACCACAGAGG TTCCTGAGGA GCAGACCCAG CCCCTACCCG AAGCCCCTAC AGTGCCTGAG 
ACCAGTGACA CGGCTGACAA GGAGGACAGT CTAGGGATCG GGGACTATGA CTACGTGCCC 
CCAGATGACT ATTACACTCC ACCCCCATAT GAAGACTTTG GATATGGCGA GGGTGTGGAG 
AACCCTGACC AGCCCACCAA CCCCGACTCA GGGGCTGAGG TCCCCACCAG CACCACTGTT 
ACCTCCAACA CCTCCAATCC AGCTCCAGGA GAAGGGAAGG ATGACCTGGG CGGCGAATTC 
ACCGAGGAAA CCATCAAGAA TCTAGAGGAA AACTACTATG ACCCGTACTT TGACCCCGAC 
TCCGACTCCA GTGTCTCTCC ATCAGAGATA GGGCCAGGCA TGCCCGCTAA CCAGGACACC 
ATCTTTGAGG GGATTGGAGG ACCCCGAGGT GAGAAAGGGC AAAAGGGAGA ACCAGCCATC 
ATTGAGCCGG GGATGCTGAT CGAGGGGCCC CCTGGCCCTG AAGGCCCTGC TGGTCTTCCA 
GGACCTCCAG GAACTACAGG TCCTACTGGC CAAATGGGTG ACCCTGGAGA AAGGGGTCCC 
CCTGGGCGCC CAGGTCTTCC TGGAGCTGAT GGCTTGCCTG GCCCCCCAGG TACCATGCTC 
ATGCTGCCGT TCCGGTTTGG AGGCGGTGGC GATGCCGGTT CTAAGGGCCC CATGGTCTCT 
GCGCAGGAGT CCCAGGCCCA GGCTATCCTC CAGCAAGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCAA TGGGTCTCAC CGGGAGACCT GGCCCCATGG GTCCTCCTGG GAGTGGCGGT 
TTGAAAGGTG AGCCAGGAGA CATGGGACCT CAGGGTCCTC GAGGTGTGCA AGGCCCACCT 
GGCCCAACAG GGAAGCCTGG AAGACGGGGC CGTGCTGGAA GTGATGGAGC CAGAGGCATG 
CCTGGACAAA CAGGCCCCAA GGGTGACCGT GGCTTTGATG GTCTGGCTGG GTTGCCGGGA 
GAGAAAGGCC ATAGAGGTGA CCCTGGTCCT TCTGGCCCGC CCGGAATCCC AGGAGATGAT 
GGAGAAAGGG GTGACGATGG AGAAGTTGGG CCCAGGGGAC TGCCCGGGGA GCCTGGACCA 
CGTGGTCTGC TTGGGCCAAA AGGCCCCCCA GGGCCTCCTG GACCTCCTGG TGTAACGGGT 
ATGGATGGCC AGCCTGGCCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGGCCGCCA 
GGACAGCAGG GTAATCCTGG TGCCCAGGGT CTTCCAGGTC CCCAGGGTGC CATTGGTCCT 
CCAGGAGAAA AGGGTCCTTT GGGGAAACCA GGTCTCCCAG GAATGCCAGG CGCTGATGGA 
CCCCCGGGGC ACCCTGGAAA AGAAGGTCCT CCAGGAGAGA AAGGAGGCCA GGGTCCTCCT 
GGCCCCCAGG GTCCCATTGG CTACCCCGGT CCACGAGGAG TCAAGGGGGC AGATGGCATC 
CGAGGTCTGA AGGGCACCAA GGGGGAGAAG GGTGAAGACG GCTTCCCTGG GTTTAAAGGC 
GACATGGGAA TAAAGGGTGA CCGGGGGGAA ATCGGCCCAC CTGGTCCCCG AGGAGAAGAT 
GGTCCTGAAG GTCCAAAGGG TCGAGGTGGT CCCAATGGTG ATCCTGGTCC CCTGGGGCCC 
ACTGGGGAAA AGGGAAAGCT TGGCGTGCCC GGATTACCGG GGTACCCAGG AAGACAAGGG 
CCAAAGGGTT CCATTGGATT CCCTGGCTTC CCGGGCGCCA ACGGAGAGAA GGGTGGCAGG 
GGGACACCTG GAAAGCCAGG ACCACGGGGA CAGAGAGGCC CAACGGGCCC GCGGGGTGAA 
CGAGGCCCAC GAGGCATCAC GGGGAAGCCT GGCCCTAAGG GCAACTCCGG AGGTGATGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AACGGACCCC AAGGTCCCAC CGGCTTTCCT 
GGACCCAAGG GTCCCCCGGG CCCACCAGGC AAGGACGGAC TCCCTGGACA CCCTGGGCAG 
AGAGGGGAGA CCGGTTTCCA AGGCAAGACT GGCCCTCCAG GGCCCCCAGG AGTGGTTGGC 
CCTCAGGGTC CCACAGGAGA GACGGGCCCC ATGGGTGAGC GTGGCCATCC TGGTCCTCCA 
GGCCCTCCTG GTGAACAGGG CCTCCCAGGT GCTGCTGGGA AAGAAGGAAC GAAGGGTGAC 
CCAGGTCCTG CTGGCCTCCC TGGGAAGGAT GGCCCTCCAG GATTGCGTGG ATTCCCTGGG 
GACCGAGGGC TACCTGGCCC CGTGGGAGCC CTTGGACTCA AAGGCAGTGA AGGCCCCCCT 
GGCCCACCAG GTCCTGCGGG TTCTCCAGGG GAGAGAGGAC CAGCTGGTGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCTCAG GGACCTCCGG GGCCTGCTGG AGAGAAAGGA 
CTTCCTGGCG AGAAAGGTCC ACAAGGCCCA GCTGGCCGAG ATGGCCTCCA AGGTCCCGTG 
GGGCTCCCTG GACCAGCCGG CCCAGTGGGT CCTCCTGGAG AAGATGGAGA TAAGGGAGAG 
ATCGGAGAGC CAGGGCAGAA GGGAAGCAAG GGCGACAAAG GCGAGCAGGG TCCTCCTGGG 
CCTACCGGTC CTCAAGGCCC GATTGGACAG CCAGGCCCTT CGGGAGCAGA TGGTGAACCT 
GGCCCTCGTG GACAGCAGGG CCTGTTTGGG CAGAAAGGAG ATGAAGGTTC AAGAGGTTTC 
CCAGGACCCC CCGGGCCAGT GGGATTGCAG GGTTTGCCAG GACCTCCAGG AGAAAAGGGC 
GAGACAGGAG ACGTGGGCCA GATGGGCCCT CCTGGTCCAC CAGGCCCCCG AGGACCCTCT 
GGAGCTCCAG GTGCCGATGG ACCACAGGGT CCTCCTGGAG GGATTGGCAA CCCTGGTGCA 
GTCGGAGAAA AGGGAGAACC TGGTGAAGCT GGAGATCCTG GCCTTCCAGG AGAAGGAGGT 
CCCCTGGGAC CTAAAGGAGA AAGAGGGGAG AAGGGAGAGG CTGGCCCCTC TGGTGCTGCT 
GGACCCCCTG GACCCAAAGG CCCTCCTGGA GATGATGGCC CCAAAGGCAG CCCTGGCCCT 
GTGGGCTTTC CTGGAGATCC TGGTCCCCCT GGAGAGCCAG GCCCCGCAGG TCAAGACGGC 
CCACCTGGTG ACAAAGGGGA CGATGGTGAA CCTGGGCAGA CGGGGTCCCC GGGCCCTACT 
GGTGAACCTG GTCCATCTGG GCCTCCAGGA AAGAGGGGTC CCCCAGGCCC TGCAGGCCCT 
GAAGGCAGGC AGGGGGAGAA AGGAGCCAAG GGAGAAGCTG GCTTAGAAGG CCCTCCTGGG 
AAGACTGGCC CCATTGGCCC CCAAGGGGCC CCTGGGAAGC CTGGCCCCGA TGGTCTCCGT 
GGAATCCCTG GTCCTGTGGG TGAGCAAGGC CTCCCAGGAT CCCCAGGCCC CGATGGTCCA 
CCCGGCCCCA TGGGTCCTCC AGGACTCCCT GGCCTCAAAG GAGACTCCGG TCCCAAAGGT 
GAAAAGGGCC ATCCAGGCCT GATTGGACTC ATCGGCCCTC CGGGAGAGCA AGGTGAAAAG 
GGTGACCGTG GACTCCCAGG CCCCCAGGGT TCATCTGGTC CTAAAGGAGA TCAGGGCATC 
ACAGGTCCTT CTGGCCCACT TGGGCCTCCT GGTCCTCCTG GCTTGCCGGG CCCTCCAGGT 
CCCAAAGGTG CTAAGGGCTC TTCGGGTCCC ACCGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACTCCCCG GCCCACCTGG CCCTCCGGGT GAGGTCATCC AGCCCCTGCC AATCCAGGCC 
TCCAGGACTC GGCGGAACAT TGATGCCAGC CAGCTCCTGG ACGATGGGGC TGGGGAGAGC 
TACGTGGATT ATGCAGATGG CATGGAAGAG ATCTTTGGTT CCCTCAACTC CCTGAAGCTG 
GAGATTGAAC AGATGAAGCG ACCACTGGGC ACCCAGCAGA ACCCAGCCCG TACCTGCAAG 
GATCTACAGC TCTGTCATCC TGACTTCCCA GATGGCGAAT ACTGGGTCGA TCCCAACCAA 
GGGTGCTCCA GGGACTCCTT CAAAGTCTAC TGCAATTTCA CAGCTGGAGG GTCCACGTGC 
GTCTTCCCTG ACAAGAAGTC TGAGGGAAGT AAAATGGCCC GGTGGCCCAA AGAACAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGT TCCCTGCTCT CCTATGTGGA TGCTGAAGGC 
AACCCCGTGG GCGTGGTACA AATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACAACTGCTA CCAGTCCGTG GCCTGGCAGG ATGCCGCCAC AGGCAGCTAT 
GATAAGGCTA TCCGCTTCTT GGGCTCCAAC GATGAGGAAA TGTCTTATGA TAACAACCCC 
TACATCCGTG CCCTGGTGGA TGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTGCTG 
GAGATCGACA CGCCCAAAGT AGAGCAAGTC CCCATTGTGG ACATCATGTT CAACGACTTT 
GGCGAAGCCT CACAGAAATT TGGATTTGAA GTGGGGCCAG CTTGCTTCCT AGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497707.1
CDS	436..5952
Translation	MDVHTRWKAARPGALLLSSPLLLFLLLLWAPPSSRAAQPADLLEMLDFHNLPSGVTKTTGFCATRRSSSEPDVAYRVSKDAQLSMPTKQLYPESGFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQLGLELGRSPVFLYEDHTGKPGPEEYPLFPGINLSDGKWHRIALSVYKKNVTLILDCKKKITKFLSRSDHPIIDTNGIVMFGSRILDDEIFEGDIQQLLFVSDNRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYPEGEGETYYYEYPYYEDPEDPGKEPAPTQKPVEAARETTEVPEEQTQPLPEAPTVPETSDTADKEDSLGIGDYDYVPPDDYYTPPPYEDFGYGEGVENPDQPTNPDSGAEVPTSTTVTSNTSNPAPGEGKDDLGGEFTEETIKNLEENYYDPYFDPDSDSSVSPSEIGPGMPANQDTIFEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTTGPTGQMGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPGPTGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGIPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPTGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGAAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGLPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGSRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGDPGLPGEGGPLGPKGERGEKGEAGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGDQGITGPSGPLGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGLPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGAGESYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGSKMARWPKEQPSTWYSQYKRGSLLSYVDAEGNPVGVVQMTFLRLLSASAHQNVTYNCYQSVAWQDAATGSYDKAIRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	XM_006497644.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type V, alpha 1 (Col5a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497644.4

ORF Insert Sequence:

ATGGACGTAC ACACCCGCTG GAAAGCTGCG CGCCCAGGCG CCCTGCTGCT GTCTTCGCCG 
CTACTCCTGT TCCTGCTGCT GCTGTGGGCG CCGCCTTCGA GCCGCGCAGC TCAGCCAGCA 
GATCTTCTGG AGATGCTAGA TTTTCACAAT TTGCCCTCAG GGGTAACGAA AACCACAGGT 
TTCTGTGCTA CTCGAAGATC TTCCAGCGAG CCGGATGTTG CCTACCGAGT CTCTAAAGAT 
GCACAGCTCA GCATGCCCAC CAAGCAGCTG TACCCTGAGT CTGGTTTTCC CGAGGACTTC 
TCCATCCTGA CAACCGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTAGT CTCCATTTAC 
AATGAGCAGG GCATCCAGCA GTTGGGGCTG GAGCTGGGCC GCTCCCCTGT CTTCCTCTAT 
GAGGACCACA CAGGGAAGCC CGGGCCTGAA GAGTATCCGC TTTTCCCTGG CATCAACTTG 
TCCGATGGCA AGTGGCACCG AATTGCTCTC AGTGTCTACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AGAAGAAGAT TACGAAGTTC CTCAGCCGCA GTGACCACCC CATAATAGAC 
ACCAATGGGA TTGTCATGTT TGGCTCCCGG ATTCTGGATG ATGAAATATT TGAGGGTGAC 
ATCCAACAGT TGCTTTTCGT CTCTGACAAC CGAGCTGCCT ATGACTACTG TGAGCACTAC 
AGCCCCGACT GTGACACTGC GGTCCCTGAC ACACCTCAGT CACAGGACCC TAACCCGGAT 
GAATATTACC CAGAAGGAGA GGGTGAGACC TATTACTATG AGTATCCATA TTATGAAGAC 
CCTGAAGACC CGGGAAAGGA GCCTGCCCCT ACTCAGAAGC CAGTGGAAGC TGCCAGAGAA 
ACCACAGAGG TTCCTGAGGA GCAGACCCAG CCCCTACCCG AAGCCCCTAC AGTGCCTGAG 
ACCAGTGACA CGGCTGACAA GGAGGACAGT CTAGGGATCG GGGACTATGA CTACGTGCCC 
CCAGATGACT ATTACACTCC ACCCCCATAT GAAGACTTTG GATATGGCGA GGGTGTGGAG 
AACCCTGACC AGCCCACCAA CCCCGACTCA GGGGCTGAGG TCCCCACCAG CACCACTGTT 
ACCTCCAACA CCTCCAATCC AGCTCCAGGA GAAGGGAAGG ATGACCTGGG CGGCGAATTC 
ACCGAGGAAA CCATCAAGAA TCTAGAGGAA AACTACTATG ACCCGTACTT TGACCCCGAC 
TCCGACTCCA GTGTCTCTCC ATCAGAGATA GGGCCAGGCA TGCCCGCTAA CCAGGACACC 
ATCTTTGAGG GGATTGGAGG ACCCCGAGGT GAGAAAGGGC AAAAGGGAGA ACCAGCCATC 
ATTGAGCCGG GGATGCTGAT CGAGGGGCCC CCTGGCCCTG AAGGCCCTGC TGGTCTTCCA 
GGACCTCCAG GAACTACAGG TCCTACTGGC CAAATGGGTG ACCCTGGAGA AAGGGGTCCC 
CCTGGGCGCC CAGGTCTTCC TGGAGCTGAT GGCTTGCCTG GCCCCCCAGG TACCATGCTC 
ATGCTGCCGT TCCGGTTTGG AGGCGGTGGC GATGCCGGTT CTAAGGGCCC CATGGTCTCT 
GCGCAGGAGT CCCAGGCCCA GGCTATCCTC CAGCAAGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCAA TGGGTCTCAC CGGGAGACCT GGCCCCATGG GTCCTCCTGG GAGTGGCGGT 
TTGAAAGGTG AGCCAGGAGA CATGGGACCT CAGGGTCCTC GAGGTGTGCA AGGCCCACCT 
GGCCCAACAG GGAAGCCTGG AAGACGGGGC CGTGCTGGAA GTGATGGAGC CAGAGGCATG 
CCTGGACAAA CAGGCCCCAA GGGTGACCGT GGCTTTGATG GTCTGGCTGG GTTGCCGGGA 
GAGAAAGGCC ATAGAGGTGA CCCTGGTCCT TCTGGCCCGC CCGGAATCCC AGGAGATGAT 
GGAGAAAGGG GTGACGATGG AGAAGTTGGG CCCAGGGGAC TGCCCGGGGA GCCTGGACCA 
CGTGGTCTGC TTGGGCCAAA AGGCCCCCCA GGGCCTCCTG GACCTCCTGG TGTAACGGGT 
ATGGATGGCC AGCCTGGCCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGGCCGCCA 
GGACAGCAGG GTAATCCTGG TGCCCAGGGT CTTCCAGGTC CCCAGGGTGC CATTGGTCCT 
CCAGGAGAAA AGGGTCCTTT GGGGAAACCA GGTCTCCCAG GAATGCCAGG CGCTGATGGA 
CCCCCGGGGC ACCCTGGAAA AGAAGGTCCT CCAGGAGAGA AAGGAGGCCA GGGTCCTCCT 
GGCCCCCAGG GTCCCATTGG CTACCCCGGT CCACGAGGAG TCAAGGGGGC AGATGGCATC 
CGAGGTCTGA AGGGCACCAA GGGGGAGAAG GGTGAAGACG GCTTCCCTGG GTTTAAAGGC 
GACATGGGAA TAAAGGGTGA CCGGGGGGAA ATCGGCCCAC CTGGTCCCCG AGGAGAAGAT 
GGTCCTGAAG GTCCAAAGGG TCGAGGTGGT CCCAATGGTG ATCCTGGTCC CCTGGGGCCC 
ACTGGGGAAA AGGGAAAGCT TGGCGTGCCC GGATTACCGG GGTACCCAGG AAGACAAGGG 
CCAAAGGGTT CCATTGGATT CCCTGGCTTC CCGGGCGCCA ACGGAGAGAA GGGTGGCAGG 
GGGACACCTG GAAAGCCAGG ACCACGGGGA CAGAGAGGCC CAACGGGCCC GCGGGGTGAA 
CGAGGCCCAC GAGGCATCAC GGGGAAGCCT GGCCCTAAGG GCAACTCCGG AGGTGATGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AACGGACCCC AAGGTCCCAC CGGCTTTCCT 
GGACCCAAGG GTCCCCCGGG CCCACCAGGC AAGGACGGAC TCCCTGGACA CCCTGGGCAG 
AGAGGGGAGA CCGGTTTCCA AGGCAAGACT GGCCCTCCAG GGCCCCCAGG AGTGGTTGGC 
CCTCAGGGTC CCACAGGAGA GACGGGCCCC ATGGGTGAGC GTGGCCATCC TGGTCCTCCA 
GGCCCTCCTG GTGAACAGGG CCTCCCAGGT GCTGCTGGGA AAGAAGGAAC GAAGGGTGAC 
CCAGGTCCTG CTGGCCTCCC TGGGAAGGAT GGCCCTCCAG GATTGCGTGG ATTCCCTGGG 
GACCGAGGGC TACCTGGCCC CGTGGGAGCC CTTGGACTCA AAGGCAGTGA AGGCCCCCCT 
GGCCCACCAG GTCCTGCGGG TTCTCCAGGG GAGAGAGGAC CAGCTGGTGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCTCAG GGACCTCCGG GGCCTGCTGG AGAGAAAGGA 
CTTCCTGGCG AGAAAGGTCC ACAAGGCCCA GCTGGCCGAG ATGGCCTCCA AGGTCCCGTG 
GGGCTCCCTG GACCAGCCGG CCCAGTGGGT CCTCCTGGAG AAGATGGAGA TAAGGGAGAG 
ATCGGAGAGC CAGGGCAGAA GGGAAGCAAG GGCGACAAAG GCGAGCAGGG TCCTCCTGGG 
CCTACCGGTC CTCAAGGCCC GATTGGACAG CCAGGCCCTT CGGGAGCAGA TGGTGAACCT 
GGCCCTCGTG GACAGCAGGG CCTGTTTGGG CAGAAAGGAG ATGAAGGTTC AAGAGGTTTC 
CCAGGACCCC CCGGGCCAGT GGGATTGCAG GGTTTGCCAG GACCTCCAGG AGAAAAGGGC 
GAGACAGGAG ACGTGGGCCA GATGGGCCCT CCTGGTCCAC CAGGCCCCCG AGGACCCTCT 
GGAGCTCCAG GTGCCGATGG ACCACAGGGT CCTCCTGGAG GGATTGGCAA CCCTGGTGCA 
GTCGGAGAAA AGGGAGAACC TGGTGAAGCT GGAGATCCTG GCCTTCCAGG AGAAGGAGGT 
CCCCTGGGAC CTAAAGGAGA AAGAGGGGAG AAGGGAGAGG CTGGCCCCTC TGGTGCTGCT 
GGACCCCCTG GACCCAAAGG CCCTCCTGGA GATGATGGCC CCAAAGGCAG CCCTGGCCCT 
GTGGGCTTTC CTGGAGATCC TGGTCCCCCT GGAGAGCCAG GCCCCGCAGG TCAAGACGGC 
CCACCTGGTG ACAAAGGGGA CGATGGTGAA CCTGGGCAGA CGGGGTCCCC GGGCCCTACT 
GGTGAACCTG GTCCATCTGG GCCTCCAGGA AAGAGGGGTC CCCCAGGCCC TGCAGGCCCT 
GAAGGCAGGC AGGGGGAGAA AGGAGCCAAG GGAGAAGCTG GCTTAGAAGG CCCTCCTGGG 
AAGACTGGCC CCATTGGCCC CCAAGGGGCC CCTGGGAAGC CTGGCCCCGA TGGTCTCCGT 
GGAATCCCTG GTCCTGTGGG TGAGCAAGGC CTCCCAGGAT CCCCAGGCCC CGATGGTCCA 
CCCGGCCCCA TGGGTCCTCC AGGACTCCCT GGCCTCAAAG GAGACTCCGG TCCCAAAGGT 
GAAAAGGGCC ATCCAGGCCT GATTGGACTC ATCGGCCCTC CGGGAGAGCA AGGTGAAAAG 
GGTGACCGTG GACTCCCAGG CCCCCAGGGT TCATCTGGTC CTAAAGGAGA TCAGGGCATC 
ACAGGTCCTT CTGGCCCACT TGGGCCTCCT GGTCCTCCTG GCTTGCCGGG CCCTCCAGGT 
CCCAAAGGTG CTAAGGGCTC TTCGGGTCCC ACCGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACTCCCCG GCCCACCTGG CCCTCCGGGT GAGGTCATCC AGCCCCTGCC AATCCAGGCC 
TCCAGGACTC GGCGGAACAT TGATGCCAGC CAGCTCCTGG ACGATGGGGC TGGGGAGAGC 
TACGTGGATT ATGCAGATGG CATGGAAGAG ATCTTTGGTT CCCTCAACTC CCTGAAGCTG 
GAGATTGAAC AGATGAAGCG ACCACTGGGC ACCCAGCAGA ACCCAGCCCG TACCTGCAAG 
GATCTACAGC TCTGTCATCC TGACTTCCCA GATGGCGAAT ACTGGGTCGA TCCCAACCAA 
GGGTGCTCCA GGGACTCCTT CAAAGTCTAC TGCAATTTCA CAGCTGGAGG GTCCACGTGC 
GTCTTCCCTG ACAAGAAGTC TGAGGGAAGT AAAATGGCCC GGTGGCCCAA AGAACAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGT TCCCTGCTCT CCTATGTGGA TGCTGAAGGC 
AACCCCGTGG GCGTGGTACA AATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACAACTGCTA CCAGTCCGTG GCCTGGCAGG ATGCCGCCAC AGGCAGCTAT 
GATAAGGCTA TCCGCTTCTT GGGCTCCAAC GATGAGGAAA TGTCTTATGA TAACAACCCC 
TACATCCGTG CCCTGGTGGA TGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTGCTG 
GAGATCGACA CGCCCAAAGT AGAGCAAGTC CCCATTGTGG ACATCATGTT CAACGACTTT 
GGCGAAGCCT CACAGAAATT TGGATTTGAA GTGGGGCCAG CTTGCTTCCT AGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col5a1 ( NM_015734.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_056549.2 Mus musculus collagen, type V, alpha 1 (Col5a1), mRNA.

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CloneID	OMu21268
Clone ID Related Accession (Same CDS sequence)	NM_015734.2
Accession Version	NM_015734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(V) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY713878.1, AB009993.1, BQ885116.1, BQ768928.1, AK148055.1, AL731778.12 and BP759660.1. On Jan 10, 2008 this sequence version replaced NM_015734.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB009993.1, BC138447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGTAC ACACCCGCTG GAAAGCTGCG CGCCCAGGCG CCCTGCTGCT GTCTTCGCCG 
CTACTCCTGT TCCTGCTGCT GCTGTGGGCG CCGCCTTCGA GCCGCGCAGC TCAGCCAGCA 
GATCTTCTGG AGATGCTAGA TTTTCACAAT TTGCCCTCAG GGGTAACGAA AACCACAGGT 
TTCTGTGCTA CTCGAAGATC TTCCAGCGAG CCGGATGTTG CCTACCGAGT CTCTAAAGAT 
GCACAGCTCA GCATGCCCAC CAAGCAGCTG TACCCTGAGT CTGGTTTTCC CGAGGACTTC 
TCCATCCTGA CAACCGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTAGT CTCCATTTAC 
AATGAGCAGG GCATCCAGCA GTTGGGGCTG GAGCTGGGCC GCTCCCCTGT CTTCCTCTAT 
GAGGACCACA CAGGGAAGCC CGGGCCTGAA GAGTATCCGC TTTTCCCTGG CATCAACTTG 
TCCGATGGCA AGTGGCACCG AATTGCTCTC AGTGTCTACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AGAAGAAGAT TACGAAGTTC CTCAGCCGCA GTGACCACCC CATAATAGAC 
ACCAATGGGA TTGTCATGTT TGGCTCCCGG ATTCTGGATG ATGAAATATT TGAGGGTGAC 
ATCCAACAGT TGCTTTTCGT CTCTGACAAC CGAGCTGCCT ATGACTACTG TGAGCACTAC 
AGCCCCGACT GTGACACTGC GGTCCCTGAC ACACCTCAGT CACAGGACCC TAACCCGGAT 
GAATATTACC CAGAAGGAGA GGGTGAGACC TATTACTATG AGTATCCATA TTATGAAGAC 
CCTGAAGACC CGGGAAAGGA GCCTGCCCCT ACTCAGAAGC CAGTGGAAGC TGCCAGAGAA 
ACCACAGAGG TTCCTGAGGA GCAGACCCAG CCCCTACCCG AAGCCCCTAC AGTGCCTGAG 
ACCAGTGACA CGGCTGACAA GGAGGACAGT CTAGGGATCG GGGACTATGA CTACGTGCCC 
CCAGATGACT ATTACACTCC ACCCCCATAT GAAGACTTTG GATATGGCGA GGGTGTGGAG 
AACCCTGACC AGCCCACCAA CCCCGACTCA GGGGCTGAGG TCCCCACCAG CACCACTGTT 
ACCTCCAACA CCTCCAATCC AGCTCCAGGA GAAGGGAAGG ATGACCTGGG CGGCGAATTC 
ACCGAGGAAA CCATCAAGAA TCTAGAGGAA AACTACTATG ACCCGTACTT TGACCCCGAC 
TCCGACTCCA GTGTCTCTCC ATCAGAGATA GGGCCAGGCA TGCCCGCTAA CCAGGACACC 
ATCTTTGAGG GGATTGGAGG ACCCCGAGGT GAGAAAGGGC AAAAGGGAGA ACCAGCCATC 
ATTGAGCCGG GGATGCTGAT CGAGGGGCCC CCTGGCCCTG AAGGCCCTGC TGGTCTTCCA 
GGACCTCCAG GAACTACAGG TCCTACTGGC CAAATGGGTG ACCCTGGAGA AAGGGGTCCC 
CCTGGGCGCC CAGGTCTTCC TGGAGCTGAT GGCTTGCCTG GCCCCCCAGG TACCATGCTC 
ATGCTGCCGT TCCGGTTTGG AGGCGGTGGC GATGCCGGTT CTAAGGGCCC CATGGTCTCT 
GCGCAGGAGT CCCAGGCCCA GGCTATCCTC CAGCAAGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCAA TGGGTCTCAC CGGGAGACCT GGCCCCATGG GTCCTCCTGG GAGTGGCGGT 
TTGAAAGGTG AGCCAGGAGA CATGGGACCT CAGGGTCCTC GAGGTGTGCA AGGCCCACCT 
GGCCCAACAG GGAAGCCTGG AAGACGGGGC CGTGCTGGAA GTGATGGAGC CAGAGGCATG 
CCTGGACAAA CAGGCCCCAA GGGTGACCGT GGCTTTGATG GTCTGGCTGG GTTGCCGGGA 
GAGAAAGGCC ATAGAGGTGA CCCTGGTCCT TCTGGCCCGC CCGGAATCCC AGGAGATGAT 
GGAGAAAGGG GTGACGATGG AGAAGTTGGG CCCAGGGGAC TGCCCGGGGA GCCTGGACCA 
CGTGGTCTGC TTGGGCCAAA AGGCCCCCCA GGGCCTCCTG GACCTCCTGG TGTAACGGGT 
ATGGATGGCC AGCCTGGCCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGGCCGCCA 
GGACAGCAGG GTAATCCTGG TGCCCAGGGT CTTCCAGGTC CCCAGGGTGC CATTGGTCCT 
CCAGGAGAAA AGGGTCCTTT GGGGAAACCA GGTCTCCCAG GAATGCCAGG CGCTGATGGA 
CCCCCGGGGC ACCCTGGAAA AGAAGGTCCT CCAGGAGAGA AAGGAGGCCA GGGTCCTCCT 
GGCCCCCAGG GTCCCATTGG CTACCCCGGT CCACGAGGAG TCAAGGGGGC AGATGGCATC 
CGAGGTCTGA AGGGCACCAA GGGGGAGAAG GGTGAAGACG GCTTCCCTGG GTTTAAAGGC 
GACATGGGAA TAAAGGGTGA CCGGGGGGAA ATCGGCCCAC CTGGTCCCCG AGGAGAAGAT 
GGTCCTGAAG GTCCAAAGGG TCGAGGTGGT CCCAATGGTG ATCCTGGTCC CCTGGGGCCC 
ACTGGGGAAA AGGGAAAGCT TGGCGTGCCC GGATTACCGG GGTACCCAGG AAGACAAGGG 
CCAAAGGGTT CCATTGGATT CCCTGGCTTC CCGGGCGCCA ACGGAGAGAA GGGTGGCAGG 
GGGACACCTG GAAAGCCAGG ACCACGGGGA CAGAGAGGCC CAACGGGCCC GCGGGGTGAA 
CGAGGCCCAC GAGGCATCAC GGGGAAGCCT GGCCCTAAGG GCAACTCCGG AGGTGATGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AACGGACCCC AAGGTCCCAC CGGCTTTCCT 
GGACCCAAGG GTCCCCCGGG CCCACCAGGC AAGGACGGAC TCCCTGGACA CCCTGGGCAG 
AGAGGGGAGA CCGGTTTCCA AGGCAAGACT GGCCCTCCAG GGCCCCCAGG AGTGGTTGGC 
CCTCAGGGTC CCACAGGAGA GACGGGCCCC ATGGGTGAGC GTGGCCATCC TGGTCCTCCA 
GGCCCTCCTG GTGAACAGGG CCTCCCAGGT GCTGCTGGGA AAGAAGGAAC GAAGGGTGAC 
CCAGGTCCTG CTGGCCTCCC TGGGAAGGAT GGCCCTCCAG GATTGCGTGG ATTCCCTGGG 
GACCGAGGGC TACCTGGCCC CGTGGGAGCC CTTGGACTCA AAGGCAGTGA AGGCCCCCCT 
GGCCCACCAG GTCCTGCGGG TTCTCCAGGG GAGAGAGGAC CAGCTGGTGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCTCAG GGACCTCCGG GGCCTGCTGG AGAGAAAGGA 
CTTCCTGGCG AGAAAGGTCC ACAAGGCCCA GCTGGCCGAG ATGGCCTCCA AGGTCCCGTG 
GGGCTCCCTG GACCAGCCGG CCCAGTGGGT CCTCCTGGAG AAGATGGAGA TAAGGGAGAG 
ATCGGAGAGC CAGGGCAGAA GGGAAGCAAG GGCGACAAAG GCGAGCAGGG TCCTCCTGGG 
CCTACCGGTC CTCAAGGCCC GATTGGACAG CCAGGCCCTT CGGGAGCAGA TGGTGAACCT 
GGCCCTCGTG GACAGCAGGG CCTGTTTGGG CAGAAAGGAG ATGAAGGTTC AAGAGGTTTC 
CCAGGACCCC CCGGGCCAGT GGGATTGCAG GGTTTGCCAG GACCTCCAGG AGAAAAGGGC 
GAGACAGGAG ACGTGGGCCA GATGGGCCCT CCTGGTCCAC CAGGCCCCCG AGGACCCTCT 
GGAGCTCCAG GTGCCGATGG ACCACAGGGT CCTCCTGGAG GGATTGGCAA CCCTGGTGCA 
GTCGGAGAAA AGGGAGAACC TGGTGAAGCT GGAGATCCTG GCCTTCCAGG AGAAGGAGGT 
CCCCTGGGAC CTAAAGGAGA AAGAGGGGAG AAGGGAGAGG CTGGCCCCTC TGGTGCTGCT 
GGACCCCCTG GACCCAAAGG CCCTCCTGGA GATGATGGCC CCAAAGGCAG CCCTGGCCCT 
GTGGGCTTTC CTGGAGATCC TGGTCCCCCT GGAGAGCCAG GCCCCGCAGG TCAAGACGGC 
CCACCTGGTG ACAAAGGGGA CGATGGTGAA CCTGGGCAGA CGGGGTCCCC GGGCCCTACT 
GGTGAACCTG GTCCATCTGG GCCTCCAGGA AAGAGGGGTC CCCCAGGCCC TGCAGGCCCT 
GAAGGCAGGC AGGGGGAGAA AGGAGCCAAG GGAGAAGCTG GCTTAGAAGG CCCTCCTGGG 
AAGACTGGCC CCATTGGCCC CCAAGGGGCC CCTGGGAAGC CTGGCCCCGA TGGTCTCCGT 
GGAATCCCTG GTCCTGTGGG TGAGCAAGGC CTCCCAGGAT CCCCAGGCCC CGATGGTCCA 
CCCGGCCCCA TGGGTCCTCC AGGACTCCCT GGCCTCAAAG GAGACTCCGG TCCCAAAGGT 
GAAAAGGGCC ATCCAGGCCT GATTGGACTC ATCGGCCCTC CGGGAGAGCA AGGTGAAAAG 
GGTGACCGTG GACTCCCAGG CCCCCAGGGT TCATCTGGTC CTAAAGGAGA TCAGGGCATC 
ACAGGTCCTT CTGGCCCACT TGGGCCTCCT GGTCCTCCTG GCTTGCCGGG CCCTCCAGGT 
CCCAAAGGTG CTAAGGGCTC TTCGGGTCCC ACCGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACTCCCCG GCCCACCTGG CCCTCCGGGT GAGGTCATCC AGCCCCTGCC AATCCAGGCC 
TCCAGGACTC GGCGGAACAT TGATGCCAGC CAGCTCCTGG ACGATGGGGC TGGGGAGAGC 
TACGTGGATT ATGCAGATGG CATGGAAGAG ATCTTTGGTT CCCTCAACTC CCTGAAGCTG 
GAGATTGAAC AGATGAAGCG ACCACTGGGC ACCCAGCAGA ACCCAGCCCG TACCTGCAAG 
GATCTACAGC TCTGTCATCC TGACTTCCCA GATGGCGAAT ACTGGGTCGA TCCCAACCAA 
GGGTGCTCCA GGGACTCCTT CAAAGTCTAC TGCAATTTCA CAGCTGGAGG GTCCACGTGC 
GTCTTCCCTG ACAAGAAGTC TGAGGGAGCC AGAATCACTT CTTGGCCCAA AGAAAACCCA 
GGTTCCTGGT TCAGTGAATT CAAGCGTGGG AAACTGCTCT CCTATGTGGA TGCTGAAGGC 
AACCCCGTGG GCGTGGTACA AATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACAACTGCTA CCAGTCCGTG GCCTGGCAGG ATGCCGCCAC AGGCAGCTAT 
GATAAGGCTA TCCGCTTCTT GGGCTCCAAC GATGAGGAAA TGTCTTATGA TAACAACCCC 
TACATCCGTG CCCTGGTGGA TGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTGCTG 
GAGATCGACA CGCCCAAAGT AGAGCAAGTC CCCATTGTGG ACATCATGTT CAACGACTTT 
GGCGAAGCCT CACAGAAATT TGGATTTGAA GTGGGGCCAG CTTGCTTCCT AGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056549.2
CDS	392..5908
Translation	MDVHTRWKAARPGALLLSSPLLLFLLLLWAPPSSRAAQPADLLEMLDFHNLPSGVTKTTGFCATRRSSSEPDVAYRVSKDAQLSMPTKQLYPESGFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQLGLELGRSPVFLYEDHTGKPGPEEYPLFPGINLSDGKWHRIALSVYKKNVTLILDCKKKITKFLSRSDHPIIDTNGIVMFGSRILDDEIFEGDIQQLLFVSDNRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYPEGEGETYYYEYPYYEDPEDPGKEPAPTQKPVEAARETTEVPEEQTQPLPEAPTVPETSDTADKEDSLGIGDYDYVPPDDYYTPPPYEDFGYGEGVENPDQPTNPDSGAEVPTSTTVTSNTSNPAPGEGKDDLGGEFTEETIKNLEENYYDPYFDPDSDSSVSPSEIGPGMPANQDTIFEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTTGPTGQMGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPGPTGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGIPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPTGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGAAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGLPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGSRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGDPGLPGEGGPLGPKGERGEKGEAGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGDQGITGPSGPLGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGLPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGAGESYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDAEGNPVGVVQMTFLRLLSASAHQNVTYNCYQSVAWQDAATGSYDKAIRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	NM_015734.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type V, alpha 1 (Col5a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015734.2

ORF Insert Sequence:

ATGGACGTAC ACACCCGCTG GAAAGCTGCG CGCCCAGGCG CCCTGCTGCT GTCTTCGCCG 
CTACTCCTGT TCCTGCTGCT GCTGTGGGCG CCGCCTTCGA GCCGCGCAGC TCAGCCAGCA 
GATCTTCTGG AGATGCTAGA TTTTCACAAT TTGCCCTCAG GGGTAACGAA AACCACAGGT 
TTCTGTGCTA CTCGAAGATC TTCCAGCGAG CCGGATGTTG CCTACCGAGT CTCTAAAGAT 
GCACAGCTCA GCATGCCCAC CAAGCAGCTG TACCCTGAGT CTGGTTTTCC CGAGGACTTC 
TCCATCCTGA CAACCGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTAGT CTCCATTTAC 
AATGAGCAGG GCATCCAGCA GTTGGGGCTG GAGCTGGGCC GCTCCCCTGT CTTCCTCTAT 
GAGGACCACA CAGGGAAGCC CGGGCCTGAA GAGTATCCGC TTTTCCCTGG CATCAACTTG 
TCCGATGGCA AGTGGCACCG AATTGCTCTC AGTGTCTACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AGAAGAAGAT TACGAAGTTC CTCAGCCGCA GTGACCACCC CATAATAGAC 
ACCAATGGGA TTGTCATGTT TGGCTCCCGG ATTCTGGATG ATGAAATATT TGAGGGTGAC 
ATCCAACAGT TGCTTTTCGT CTCTGACAAC CGAGCTGCCT ATGACTACTG TGAGCACTAC 
AGCCCCGACT GTGACACTGC GGTCCCTGAC ACACCTCAGT CACAGGACCC TAACCCGGAT 
GAATATTACC CAGAAGGAGA GGGTGAGACC TATTACTATG AGTATCCATA TTATGAAGAC 
CCTGAAGACC CGGGAAAGGA GCCTGCCCCT ACTCAGAAGC CAGTGGAAGC TGCCAGAGAA 
ACCACAGAGG TTCCTGAGGA GCAGACCCAG CCCCTACCCG AAGCCCCTAC AGTGCCTGAG 
ACCAGTGACA CGGCTGACAA GGAGGACAGT CTAGGGATCG GGGACTATGA CTACGTGCCC 
CCAGATGACT ATTACACTCC ACCCCCATAT GAAGACTTTG GATATGGCGA GGGTGTGGAG 
AACCCTGACC AGCCCACCAA CCCCGACTCA GGGGCTGAGG TCCCCACCAG CACCACTGTT 
ACCTCCAACA CCTCCAATCC AGCTCCAGGA GAAGGGAAGG ATGACCTGGG CGGCGAATTC 
ACCGAGGAAA CCATCAAGAA TCTAGAGGAA AACTACTATG ACCCGTACTT TGACCCCGAC 
TCCGACTCCA GTGTCTCTCC ATCAGAGATA GGGCCAGGCA TGCCCGCTAA CCAGGACACC 
ATCTTTGAGG GGATTGGAGG ACCCCGAGGT GAGAAAGGGC AAAAGGGAGA ACCAGCCATC 
ATTGAGCCGG GGATGCTGAT CGAGGGGCCC CCTGGCCCTG AAGGCCCTGC TGGTCTTCCA 
GGACCTCCAG GAACTACAGG TCCTACTGGC CAAATGGGTG ACCCTGGAGA AAGGGGTCCC 
CCTGGGCGCC CAGGTCTTCC TGGAGCTGAT GGCTTGCCTG GCCCCCCAGG TACCATGCTC 
ATGCTGCCGT TCCGGTTTGG AGGCGGTGGC GATGCCGGTT CTAAGGGCCC CATGGTCTCT 
GCGCAGGAGT CCCAGGCCCA GGCTATCCTC CAGCAAGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCAA TGGGTCTCAC CGGGAGACCT GGCCCCATGG GTCCTCCTGG GAGTGGCGGT 
TTGAAAGGTG AGCCAGGAGA CATGGGACCT CAGGGTCCTC GAGGTGTGCA AGGCCCACCT 
GGCCCAACAG GGAAGCCTGG AAGACGGGGC CGTGCTGGAA GTGATGGAGC CAGAGGCATG 
CCTGGACAAA CAGGCCCCAA GGGTGACCGT GGCTTTGATG GTCTGGCTGG GTTGCCGGGA 
GAGAAAGGCC ATAGAGGTGA CCCTGGTCCT TCTGGCCCGC CCGGAATCCC AGGAGATGAT 
GGAGAAAGGG GTGACGATGG AGAAGTTGGG CCCAGGGGAC TGCCCGGGGA GCCTGGACCA 
CGTGGTCTGC TTGGGCCAAA AGGCCCCCCA GGGCCTCCTG GACCTCCTGG TGTAACGGGT 
ATGGATGGCC AGCCTGGCCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGGCCGCCA 
GGACAGCAGG GTAATCCTGG TGCCCAGGGT CTTCCAGGTC CCCAGGGTGC CATTGGTCCT 
CCAGGAGAAA AGGGTCCTTT GGGGAAACCA GGTCTCCCAG GAATGCCAGG CGCTGATGGA 
CCCCCGGGGC ACCCTGGAAA AGAAGGTCCT CCAGGAGAGA AAGGAGGCCA GGGTCCTCCT 
GGCCCCCAGG GTCCCATTGG CTACCCCGGT CCACGAGGAG TCAAGGGGGC AGATGGCATC 
CGAGGTCTGA AGGGCACCAA GGGGGAGAAG GGTGAAGACG GCTTCCCTGG GTTTAAAGGC 
GACATGGGAA TAAAGGGTGA CCGGGGGGAA ATCGGCCCAC CTGGTCCCCG AGGAGAAGAT 
GGTCCTGAAG GTCCAAAGGG TCGAGGTGGT CCCAATGGTG ATCCTGGTCC CCTGGGGCCC 
ACTGGGGAAA AGGGAAAGCT TGGCGTGCCC GGATTACCGG GGTACCCAGG AAGACAAGGG 
CCAAAGGGTT CCATTGGATT CCCTGGCTTC CCGGGCGCCA ACGGAGAGAA GGGTGGCAGG 
GGGACACCTG GAAAGCCAGG ACCACGGGGA CAGAGAGGCC CAACGGGCCC GCGGGGTGAA 
CGAGGCCCAC GAGGCATCAC GGGGAAGCCT GGCCCTAAGG GCAACTCCGG AGGTGATGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AACGGACCCC AAGGTCCCAC CGGCTTTCCT 
GGACCCAAGG GTCCCCCGGG CCCACCAGGC AAGGACGGAC TCCCTGGACA CCCTGGGCAG 
AGAGGGGAGA CCGGTTTCCA AGGCAAGACT GGCCCTCCAG GGCCCCCAGG AGTGGTTGGC 
CCTCAGGGTC CCACAGGAGA GACGGGCCCC ATGGGTGAGC GTGGCCATCC TGGTCCTCCA 
GGCCCTCCTG GTGAACAGGG CCTCCCAGGT GCTGCTGGGA AAGAAGGAAC GAAGGGTGAC 
CCAGGTCCTG CTGGCCTCCC TGGGAAGGAT GGCCCTCCAG GATTGCGTGG ATTCCCTGGG 
GACCGAGGGC TACCTGGCCC CGTGGGAGCC CTTGGACTCA AAGGCAGTGA AGGCCCCCCT 
GGCCCACCAG GTCCTGCGGG TTCTCCAGGG GAGAGAGGAC CAGCTGGTGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCTCAG GGACCTCCGG GGCCTGCTGG AGAGAAAGGA 
CTTCCTGGCG AGAAAGGTCC ACAAGGCCCA GCTGGCCGAG ATGGCCTCCA AGGTCCCGTG 
GGGCTCCCTG GACCAGCCGG CCCAGTGGGT CCTCCTGGAG AAGATGGAGA TAAGGGAGAG 
ATCGGAGAGC CAGGGCAGAA GGGAAGCAAG GGCGACAAAG GCGAGCAGGG TCCTCCTGGG 
CCTACCGGTC CTCAAGGCCC GATTGGACAG CCAGGCCCTT CGGGAGCAGA TGGTGAACCT 
GGCCCTCGTG GACAGCAGGG CCTGTTTGGG CAGAAAGGAG ATGAAGGTTC AAGAGGTTTC 
CCAGGACCCC CCGGGCCAGT GGGATTGCAG GGTTTGCCAG GACCTCCAGG AGAAAAGGGC 
GAGACAGGAG ACGTGGGCCA GATGGGCCCT CCTGGTCCAC CAGGCCCCCG AGGACCCTCT 
GGAGCTCCAG GTGCCGATGG ACCACAGGGT CCTCCTGGAG GGATTGGCAA CCCTGGTGCA 
GTCGGAGAAA AGGGAGAACC TGGTGAAGCT GGAGATCCTG GCCTTCCAGG AGAAGGAGGT 
CCCCTGGGAC CTAAAGGAGA AAGAGGGGAG AAGGGAGAGG CTGGCCCCTC TGGTGCTGCT 
GGACCCCCTG GACCCAAAGG CCCTCCTGGA GATGATGGCC CCAAAGGCAG CCCTGGCCCT 
GTGGGCTTTC CTGGAGATCC TGGTCCCCCT GGAGAGCCAG GCCCCGCAGG TCAAGACGGC 
CCACCTGGTG ACAAAGGGGA CGATGGTGAA CCTGGGCAGA CGGGGTCCCC GGGCCCTACT 
GGTGAACCTG GTCCATCTGG GCCTCCAGGA AAGAGGGGTC CCCCAGGCCC TGCAGGCCCT 
GAAGGCAGGC AGGGGGAGAA AGGAGCCAAG GGAGAAGCTG GCTTAGAAGG CCCTCCTGGG 
AAGACTGGCC CCATTGGCCC CCAAGGGGCC CCTGGGAAGC CTGGCCCCGA TGGTCTCCGT 
GGAATCCCTG GTCCTGTGGG TGAGCAAGGC CTCCCAGGAT CCCCAGGCCC CGATGGTCCA 
CCCGGCCCCA TGGGTCCTCC AGGACTCCCT GGCCTCAAAG GAGACTCCGG TCCCAAAGGT 
GAAAAGGGCC ATCCAGGCCT GATTGGACTC ATCGGCCCTC CGGGAGAGCA AGGTGAAAAG 
GGTGACCGTG GACTCCCAGG CCCCCAGGGT TCATCTGGTC CTAAAGGAGA TCAGGGCATC 
ACAGGTCCTT CTGGCCCACT TGGGCCTCCT GGTCCTCCTG GCTTGCCGGG CCCTCCAGGT 
CCCAAAGGTG CTAAGGGCTC TTCGGGTCCC ACCGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACTCCCCG GCCCACCTGG CCCTCCGGGT GAGGTCATCC AGCCCCTGCC AATCCAGGCC 
TCCAGGACTC GGCGGAACAT TGATGCCAGC CAGCTCCTGG ACGATGGGGC TGGGGAGAGC 
TACGTGGATT ATGCAGATGG CATGGAAGAG ATCTTTGGTT CCCTCAACTC CCTGAAGCTG 
GAGATTGAAC AGATGAAGCG ACCACTGGGC ACCCAGCAGA ACCCAGCCCG TACCTGCAAG 
GATCTACAGC TCTGTCATCC TGACTTCCCA GATGGCGAAT ACTGGGTCGA TCCCAACCAA 
GGGTGCTCCA GGGACTCCTT CAAAGTCTAC TGCAATTTCA CAGCTGGAGG GTCCACGTGC 
GTCTTCCCTG ACAAGAAGTC TGAGGGAGCC AGAATCACTT CTTGGCCCAA AGAAAACCCA 
GGTTCCTGGT TCAGTGAATT CAAGCGTGGG AAACTGCTCT CCTATGTGGA TGCTGAAGGC 
AACCCCGTGG GCGTGGTACA AATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACAACTGCTA CCAGTCCGTG GCCTGGCAGG ATGCCGCCAC AGGCAGCTAT 
GATAAGGCTA TCCGCTTCTT GGGCTCCAAC GATGAGGAAA TGTCTTATGA TAACAACCCC 
TACATCCGTG CCCTGGTGGA TGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTGCTG 
GAGATCGACA CGCCCAAAGT AGAGCAAGTC CCCATTGTGG ACATCATGTT CAACGACTTT 
GGCGAAGCCT CACAGAAATT TGGATTTGAA GTGGGGCCAG CTTGCTTCCT AGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col5a1 ( XM_006497644.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497707.1 Mus musculus collagen, type V, alpha 1 (Col5a1), transcript variant X1, mRNA.

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CloneID	OMu29274
Clone ID Related Accession (Same CDS sequence)	XM_006497644.3 , XM_006497644.4
Accession Version	XM_006497644.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497644.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTAC ACACCCGCTG GAAAGCTGCG CGCCCAGGCG CCCTGCTGCT GTCTTCGCCG 
CTACTCCTGT TCCTGCTGCT GCTGTGGGCG CCGCCTTCGA GCCGCGCAGC TCAGCCAGCA 
GATCTTCTGG AGATGCTAGA TTTTCACAAT TTGCCCTCAG GGGTAACGAA AACCACAGGT 
TTCTGTGCTA CTCGAAGATC TTCCAGCGAG CCGGATGTTG CCTACCGAGT CTCTAAAGAT 
GCACAGCTCA GCATGCCCAC CAAGCAGCTG TACCCTGAGT CTGGTTTTCC CGAGGACTTC 
TCCATCCTGA CAACCGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTAGT CTCCATTTAC 
AATGAGCAGG GCATCCAGCA GTTGGGGCTG GAGCTGGGCC GCTCCCCTGT CTTCCTCTAT 
GAGGACCACA CAGGGAAGCC CGGGCCTGAA GAGTATCCGC TTTTCCCTGG CATCAACTTG 
TCCGATGGCA AGTGGCACCG AATTGCTCTC AGTGTCTACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AGAAGAAGAT TACGAAGTTC CTCAGCCGCA GTGACCACCC CATAATAGAC 
ACCAATGGGA TTGTCATGTT TGGCTCCCGG ATTCTGGATG ATGAAATATT TGAGGGTGAC 
ATCCAACAGT TGCTTTTCGT CTCTGACAAC CGAGCTGCCT ATGACTACTG TGAGCACTAC 
AGCCCCGACT GTGACACTGC GGTCCCTGAC ACACCTCAGT CACAGGACCC TAACCCGGAT 
GAATATTACC CAGAAGGAGA GGGTGAGACC TATTACTATG AGTATCCATA TTATGAAGAC 
CCTGAAGACC CGGGAAAGGA GCCTGCCCCT ACTCAGAAGC CAGTGGAAGC TGCCAGAGAA 
ACCACAGAGG TTCCTGAGGA GCAGACCCAG CCCCTACCCG AAGCCCCTAC AGTGCCTGAG 
ACCAGTGACA CGGCTGACAA GGAGGACAGT CTAGGGATCG GGGACTATGA CTACGTGCCC 
CCAGATGACT ATTACACTCC ACCCCCATAT GAAGACTTTG GATATGGCGA GGGTGTGGAG 
AACCCTGACC AGCCCACCAA CCCCGACTCA GGGGCTGAGG TCCCCACCAG CACCACTGTT 
ACCTCCAACA CCTCCAATCC AGCTCCAGGA GAAGGGAAGG ATGACCTGGG CGGCGAATTC 
ACCGAGGAAA CCATCAAGAA TCTAGAGGAA AACTACTATG ACCCGTACTT TGACCCCGAC 
TCCGACTCCA GTGTCTCTCC ATCAGAGATA GGGCCAGGCA TGCCCGCTAA CCAGGACACC 
ATCTTTGAGG GGATTGGAGG ACCCCGAGGT GAGAAAGGGC AAAAGGGAGA ACCAGCCATC 
ATTGAGCCGG GGATGCTGAT CGAGGGGCCC CCTGGCCCTG AAGGCCCTGC TGGTCTTCCA 
GGACCTCCAG GAACTACAGG TCCTACTGGC CAAATGGGTG ACCCTGGAGA AAGGGGTCCC 
CCTGGGCGCC CAGGTCTTCC TGGAGCTGAT GGCTTGCCTG GCCCCCCAGG TACCATGCTC 
ATGCTGCCGT TCCGGTTTGG AGGCGGTGGC GATGCCGGTT CTAAGGGCCC CATGGTCTCT 
GCGCAGGAGT CCCAGGCCCA GGCTATCCTC CAGCAAGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCAA TGGGTCTCAC CGGGAGACCT GGCCCCATGG GTCCTCCTGG GAGTGGCGGT 
TTGAAAGGTG AGCCAGGAGA CATGGGACCT CAGGGTCCTC GAGGTGTGCA AGGCCCACCT 
GGCCCAACAG GGAAGCCTGG AAGACGGGGC CGTGCTGGAA GTGATGGAGC CAGAGGCATG 
CCTGGACAAA CAGGCCCCAA GGGTGACCGT GGCTTTGATG GTCTGGCTGG GTTGCCGGGA 
GAGAAAGGCC ATAGAGGTGA CCCTGGTCCT TCTGGCCCGC CCGGAATCCC AGGAGATGAT 
GGAGAAAGGG GTGACGATGG AGAAGTTGGG CCCAGGGGAC TGCCCGGGGA GCCTGGACCA 
CGTGGTCTGC TTGGGCCAAA AGGCCCCCCA GGGCCTCCTG GACCTCCTGG TGTAACGGGT 
ATGGATGGCC AGCCTGGCCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGGCCGCCA 
GGACAGCAGG GTAATCCTGG TGCCCAGGGT CTTCCAGGTC CCCAGGGTGC CATTGGTCCT 
CCAGGAGAAA AGGGTCCTTT GGGGAAACCA GGTCTCCCAG GAATGCCAGG CGCTGATGGA 
CCCCCGGGGC ACCCTGGAAA AGAAGGTCCT CCAGGAGAGA AAGGAGGCCA GGGTCCTCCT 
GGCCCCCAGG GTCCCATTGG CTACCCCGGT CCACGAGGAG TCAAGGGGGC AGATGGCATC 
CGAGGTCTGA AGGGCACCAA GGGGGAGAAG GGTGAAGACG GCTTCCCTGG GTTTAAAGGC 
GACATGGGAA TAAAGGGTGA CCGGGGGGAA ATCGGCCCAC CTGGTCCCCG AGGAGAAGAT 
GGTCCTGAAG GTCCAAAGGG TCGAGGTGGT CCCAATGGTG ATCCTGGTCC CCTGGGGCCC 
ACTGGGGAAA AGGGAAAGCT TGGCGTGCCC GGATTACCGG GGTACCCAGG AAGACAAGGG 
CCAAAGGGTT CCATTGGATT CCCTGGCTTC CCGGGCGCCA ACGGAGAGAA GGGTGGCAGG 
GGGACACCTG GAAAGCCAGG ACCACGGGGA CAGAGAGGCC CAACGGGCCC GCGGGGTGAA 
CGAGGCCCAC GAGGCATCAC GGGGAAGCCT GGCCCTAAGG GCAACTCCGG AGGTGATGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AACGGACCCC AAGGTCCCAC CGGCTTTCCT 
GGACCCAAGG GTCCCCCGGG CCCACCAGGC AAGGACGGAC TCCCTGGACA CCCTGGGCAG 
AGAGGGGAGA CCGGTTTCCA AGGCAAGACT GGCCCTCCAG GGCCCCCAGG AGTGGTTGGC 
CCTCAGGGTC CCACAGGAGA GACGGGCCCC ATGGGTGAGC GTGGCCATCC TGGTCCTCCA 
GGCCCTCCTG GTGAACAGGG CCTCCCAGGT GCTGCTGGGA AAGAAGGAAC GAAGGGTGAC 
CCAGGTCCTG CTGGCCTCCC TGGGAAGGAT GGCCCTCCAG GATTGCGTGG ATTCCCTGGG 
GACCGAGGGC TACCTGGCCC CGTGGGAGCC CTTGGACTCA AAGGCAGTGA AGGCCCCCCT 
GGCCCACCAG GTCCTGCGGG TTCTCCAGGG GAGAGAGGAC CAGCTGGTGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCTCAG GGACCTCCGG GGCCTGCTGG AGAGAAAGGA 
CTTCCTGGCG AGAAAGGTCC ACAAGGCCCA GCTGGCCGAG ATGGCCTCCA AGGTCCCGTG 
GGGCTCCCTG GACCAGCCGG CCCAGTGGGT CCTCCTGGAG AAGATGGAGA TAAGGGAGAG 
ATCGGAGAGC CAGGGCAGAA GGGAAGCAAG GGCGACAAAG GCGAGCAGGG TCCTCCTGGG 
CCTACCGGTC CTCAAGGCCC GATTGGACAG CCAGGCCCTT CGGGAGCAGA TGGTGAACCT 
GGCCCTCGTG GACAGCAGGG CCTGTTTGGG CAGAAAGGAG ATGAAGGTTC AAGAGGTTTC 
CCAGGACCCC CCGGGCCAGT GGGATTGCAG GGTTTGCCAG GACCTCCAGG AGAAAAGGGC 
GAGACAGGAG ACGTGGGCCA GATGGGCCCT CCTGGTCCAC CAGGCCCCCG AGGACCCTCT 
GGAGCTCCAG GTGCCGATGG ACCACAGGGT CCTCCTGGAG GGATTGGCAA CCCTGGTGCA 
GTCGGAGAAA AGGGAGAACC TGGTGAAGCT GGAGATCCTG GCCTTCCAGG AGAAGGAGGT 
CCCCTGGGAC CTAAAGGAGA AAGAGGGGAG AAGGGAGAGG CTGGCCCCTC TGGTGCTGCT 
GGACCCCCTG GACCCAAAGG CCCTCCTGGA GATGATGGCC CCAAAGGCAG CCCTGGCCCT 
GTGGGCTTTC CTGGAGATCC TGGTCCCCCT GGAGAGCCAG GCCCCGCAGG TCAAGACGGC 
CCACCTGGTG ACAAAGGGGA CGATGGTGAA CCTGGGCAGA CGGGGTCCCC GGGCCCTACT 
GGTGAACCTG GTCCATCTGG GCCTCCAGGA AAGAGGGGTC CCCCAGGCCC TGCAGGCCCT 
GAAGGCAGGC AGGGGGAGAA AGGAGCCAAG GGAGAAGCTG GCTTAGAAGG CCCTCCTGGG 
AAGACTGGCC CCATTGGCCC CCAAGGGGCC CCTGGGAAGC CTGGCCCCGA TGGTCTCCGT 
GGAATCCCTG GTCCTGTGGG TGAGCAAGGC CTCCCAGGAT CCCCAGGCCC CGATGGTCCA 
CCCGGCCCCA TGGGTCCTCC AGGACTCCCT GGCCTCAAAG GAGACTCCGG TCCCAAAGGT 
GAAAAGGGCC ATCCAGGCCT GATTGGACTC ATCGGCCCTC CGGGAGAGCA AGGTGAAAAG 
GGTGACCGTG GACTCCCAGG CCCCCAGGGT TCATCTGGTC CTAAAGGAGA TCAGGGCATC 
ACAGGTCCTT CTGGCCCACT TGGGCCTCCT GGTCCTCCTG GCTTGCCGGG CCCTCCAGGT 
CCCAAAGGTG CTAAGGGCTC TTCGGGTCCC ACCGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACTCCCCG GCCCACCTGG CCCTCCGGGT GAGGTCATCC AGCCCCTGCC AATCCAGGCC 
TCCAGGACTC GGCGGAACAT TGATGCCAGC CAGCTCCTGG ACGATGGGGC TGGGGAGAGC 
TACGTGGATT ATGCAGATGG CATGGAAGAG ATCTTTGGTT CCCTCAACTC CCTGAAGCTG 
GAGATTGAAC AGATGAAGCG ACCACTGGGC ACCCAGCAGA ACCCAGCCCG TACCTGCAAG 
GATCTACAGC TCTGTCATCC TGACTTCCCA GATGGCGAAT ACTGGGTCGA TCCCAACCAA 
GGGTGCTCCA GGGACTCCTT CAAAGTCTAC TGCAATTTCA CAGCTGGAGG GTCCACGTGC 
GTCTTCCCTG ACAAGAAGTC TGAGGGAAGT AAAATGGCCC GGTGGCCCAA AGAACAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGT TCCCTGCTCT CCTATGTGGA TGCTGAAGGC 
AACCCCGTGG GCGTGGTACA AATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACAACTGCTA CCAGTCCGTG GCCTGGCAGG ATGCCGCCAC AGGCAGCTAT 
GATAAGGCTA TCCGCTTCTT GGGCTCCAAC GATGAGGAAA TGTCTTATGA TAACAACCCC 
TACATCCGTG CCCTGGTGGA TGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTGCTG 
GAGATCGACA CGCCCAAAGT AGAGCAAGTC CCCATTGTGG ACATCATGTT CAACGACTTT 
GGCGAAGCCT CACAGAAATT TGGATTTGAA GTGGGGCCAG CTTGCTTCCT AGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497707.1
CDS	422..5938
Translation	MDVHTRWKAARPGALLLSSPLLLFLLLLWAPPSSRAAQPADLLEMLDFHNLPSGVTKTTGFCATRRSSSEPDVAYRVSKDAQLSMPTKQLYPESGFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQLGLELGRSPVFLYEDHTGKPGPEEYPLFPGINLSDGKWHRIALSVYKKNVTLILDCKKKITKFLSRSDHPIIDTNGIVMFGSRILDDEIFEGDIQQLLFVSDNRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYPEGEGETYYYEYPYYEDPEDPGKEPAPTQKPVEAARETTEVPEEQTQPLPEAPTVPETSDTADKEDSLGIGDYDYVPPDDYYTPPPYEDFGYGEGVENPDQPTNPDSGAEVPTSTTVTSNTSNPAPGEGKDDLGGEFTEETIKNLEENYYDPYFDPDSDSSVSPSEIGPGMPANQDTIFEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTTGPTGQMGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPGPTGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGIPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPTGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGAAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGLPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGSRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGDPGLPGEGGPLGPKGERGEKGEAGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGDQGITGPSGPLGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGLPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGAGESYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGSKMARWPKEQPSTWYSQYKRGSLLSYVDAEGNPVGVVQMTFLRLLSASAHQNVTYNCYQSVAWQDAATGSYDKAIRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	XM_006497644.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type V, alpha 1 (Col5a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497644.3

ORF Insert Sequence:

ATGGACGTAC ACACCCGCTG GAAAGCTGCG CGCCCAGGCG CCCTGCTGCT GTCTTCGCCG 
CTACTCCTGT TCCTGCTGCT GCTGTGGGCG CCGCCTTCGA GCCGCGCAGC TCAGCCAGCA 
GATCTTCTGG AGATGCTAGA TTTTCACAAT TTGCCCTCAG GGGTAACGAA AACCACAGGT 
TTCTGTGCTA CTCGAAGATC TTCCAGCGAG CCGGATGTTG CCTACCGAGT CTCTAAAGAT 
GCACAGCTCA GCATGCCCAC CAAGCAGCTG TACCCTGAGT CTGGTTTTCC CGAGGACTTC 
TCCATCCTGA CAACCGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTAGT CTCCATTTAC 
AATGAGCAGG GCATCCAGCA GTTGGGGCTG GAGCTGGGCC GCTCCCCTGT CTTCCTCTAT 
GAGGACCACA CAGGGAAGCC CGGGCCTGAA GAGTATCCGC TTTTCCCTGG CATCAACTTG 
TCCGATGGCA AGTGGCACCG AATTGCTCTC AGTGTCTACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AGAAGAAGAT TACGAAGTTC CTCAGCCGCA GTGACCACCC CATAATAGAC 
ACCAATGGGA TTGTCATGTT TGGCTCCCGG ATTCTGGATG ATGAAATATT TGAGGGTGAC 
ATCCAACAGT TGCTTTTCGT CTCTGACAAC CGAGCTGCCT ATGACTACTG TGAGCACTAC 
AGCCCCGACT GTGACACTGC GGTCCCTGAC ACACCTCAGT CACAGGACCC TAACCCGGAT 
GAATATTACC CAGAAGGAGA GGGTGAGACC TATTACTATG AGTATCCATA TTATGAAGAC 
CCTGAAGACC CGGGAAAGGA GCCTGCCCCT ACTCAGAAGC CAGTGGAAGC TGCCAGAGAA 
ACCACAGAGG TTCCTGAGGA GCAGACCCAG CCCCTACCCG AAGCCCCTAC AGTGCCTGAG 
ACCAGTGACA CGGCTGACAA GGAGGACAGT CTAGGGATCG GGGACTATGA CTACGTGCCC 
CCAGATGACT ATTACACTCC ACCCCCATAT GAAGACTTTG GATATGGCGA GGGTGTGGAG 
AACCCTGACC AGCCCACCAA CCCCGACTCA GGGGCTGAGG TCCCCACCAG CACCACTGTT 
ACCTCCAACA CCTCCAATCC AGCTCCAGGA GAAGGGAAGG ATGACCTGGG CGGCGAATTC 
ACCGAGGAAA CCATCAAGAA TCTAGAGGAA AACTACTATG ACCCGTACTT TGACCCCGAC 
TCCGACTCCA GTGTCTCTCC ATCAGAGATA GGGCCAGGCA TGCCCGCTAA CCAGGACACC 
ATCTTTGAGG GGATTGGAGG ACCCCGAGGT GAGAAAGGGC AAAAGGGAGA ACCAGCCATC 
ATTGAGCCGG GGATGCTGAT CGAGGGGCCC CCTGGCCCTG AAGGCCCTGC TGGTCTTCCA 
GGACCTCCAG GAACTACAGG TCCTACTGGC CAAATGGGTG ACCCTGGAGA AAGGGGTCCC 
CCTGGGCGCC CAGGTCTTCC TGGAGCTGAT GGCTTGCCTG GCCCCCCAGG TACCATGCTC 
ATGCTGCCGT TCCGGTTTGG AGGCGGTGGC GATGCCGGTT CTAAGGGCCC CATGGTCTCT 
GCGCAGGAGT CCCAGGCCCA GGCTATCCTC CAGCAAGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCAA TGGGTCTCAC CGGGAGACCT GGCCCCATGG GTCCTCCTGG GAGTGGCGGT 
TTGAAAGGTG AGCCAGGAGA CATGGGACCT CAGGGTCCTC GAGGTGTGCA AGGCCCACCT 
GGCCCAACAG GGAAGCCTGG AAGACGGGGC CGTGCTGGAA GTGATGGAGC CAGAGGCATG 
CCTGGACAAA CAGGCCCCAA GGGTGACCGT GGCTTTGATG GTCTGGCTGG GTTGCCGGGA 
GAGAAAGGCC ATAGAGGTGA CCCTGGTCCT TCTGGCCCGC CCGGAATCCC AGGAGATGAT 
GGAGAAAGGG GTGACGATGG AGAAGTTGGG CCCAGGGGAC TGCCCGGGGA GCCTGGACCA 
CGTGGTCTGC TTGGGCCAAA AGGCCCCCCA GGGCCTCCTG GACCTCCTGG TGTAACGGGT 
ATGGATGGCC AGCCTGGCCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGGCCGCCA 
GGACAGCAGG GTAATCCTGG TGCCCAGGGT CTTCCAGGTC CCCAGGGTGC CATTGGTCCT 
CCAGGAGAAA AGGGTCCTTT GGGGAAACCA GGTCTCCCAG GAATGCCAGG CGCTGATGGA 
CCCCCGGGGC ACCCTGGAAA AGAAGGTCCT CCAGGAGAGA AAGGAGGCCA GGGTCCTCCT 
GGCCCCCAGG GTCCCATTGG CTACCCCGGT CCACGAGGAG TCAAGGGGGC AGATGGCATC 
CGAGGTCTGA AGGGCACCAA GGGGGAGAAG GGTGAAGACG GCTTCCCTGG GTTTAAAGGC 
GACATGGGAA TAAAGGGTGA CCGGGGGGAA ATCGGCCCAC CTGGTCCCCG AGGAGAAGAT 
GGTCCTGAAG GTCCAAAGGG TCGAGGTGGT CCCAATGGTG ATCCTGGTCC CCTGGGGCCC 
ACTGGGGAAA AGGGAAAGCT TGGCGTGCCC GGATTACCGG GGTACCCAGG AAGACAAGGG 
CCAAAGGGTT CCATTGGATT CCCTGGCTTC CCGGGCGCCA ACGGAGAGAA GGGTGGCAGG 
GGGACACCTG GAAAGCCAGG ACCACGGGGA CAGAGAGGCC CAACGGGCCC GCGGGGTGAA 
CGAGGCCCAC GAGGCATCAC GGGGAAGCCT GGCCCTAAGG GCAACTCCGG AGGTGATGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AACGGACCCC AAGGTCCCAC CGGCTTTCCT 
GGACCCAAGG GTCCCCCGGG CCCACCAGGC AAGGACGGAC TCCCTGGACA CCCTGGGCAG 
AGAGGGGAGA CCGGTTTCCA AGGCAAGACT GGCCCTCCAG GGCCCCCAGG AGTGGTTGGC 
CCTCAGGGTC CCACAGGAGA GACGGGCCCC ATGGGTGAGC GTGGCCATCC TGGTCCTCCA 
GGCCCTCCTG GTGAACAGGG CCTCCCAGGT GCTGCTGGGA AAGAAGGAAC GAAGGGTGAC 
CCAGGTCCTG CTGGCCTCCC TGGGAAGGAT GGCCCTCCAG GATTGCGTGG ATTCCCTGGG 
GACCGAGGGC TACCTGGCCC CGTGGGAGCC CTTGGACTCA AAGGCAGTGA AGGCCCCCCT 
GGCCCACCAG GTCCTGCGGG TTCTCCAGGG GAGAGAGGAC CAGCTGGTGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCTCAG GGACCTCCGG GGCCTGCTGG AGAGAAAGGA 
CTTCCTGGCG AGAAAGGTCC ACAAGGCCCA GCTGGCCGAG ATGGCCTCCA AGGTCCCGTG 
GGGCTCCCTG GACCAGCCGG CCCAGTGGGT CCTCCTGGAG AAGATGGAGA TAAGGGAGAG 
ATCGGAGAGC CAGGGCAGAA GGGAAGCAAG GGCGACAAAG GCGAGCAGGG TCCTCCTGGG 
CCTACCGGTC CTCAAGGCCC GATTGGACAG CCAGGCCCTT CGGGAGCAGA TGGTGAACCT 
GGCCCTCGTG GACAGCAGGG CCTGTTTGGG CAGAAAGGAG ATGAAGGTTC AAGAGGTTTC 
CCAGGACCCC CCGGGCCAGT GGGATTGCAG GGTTTGCCAG GACCTCCAGG AGAAAAGGGC 
GAGACAGGAG ACGTGGGCCA GATGGGCCCT CCTGGTCCAC CAGGCCCCCG AGGACCCTCT 
GGAGCTCCAG GTGCCGATGG ACCACAGGGT CCTCCTGGAG GGATTGGCAA CCCTGGTGCA 
GTCGGAGAAA AGGGAGAACC TGGTGAAGCT GGAGATCCTG GCCTTCCAGG AGAAGGAGGT 
CCCCTGGGAC CTAAAGGAGA AAGAGGGGAG AAGGGAGAGG CTGGCCCCTC TGGTGCTGCT 
GGACCCCCTG GACCCAAAGG CCCTCCTGGA GATGATGGCC CCAAAGGCAG CCCTGGCCCT 
GTGGGCTTTC CTGGAGATCC TGGTCCCCCT GGAGAGCCAG GCCCCGCAGG TCAAGACGGC 
CCACCTGGTG ACAAAGGGGA CGATGGTGAA CCTGGGCAGA CGGGGTCCCC GGGCCCTACT 
GGTGAACCTG GTCCATCTGG GCCTCCAGGA AAGAGGGGTC CCCCAGGCCC TGCAGGCCCT 
GAAGGCAGGC AGGGGGAGAA AGGAGCCAAG GGAGAAGCTG GCTTAGAAGG CCCTCCTGGG 
AAGACTGGCC CCATTGGCCC CCAAGGGGCC CCTGGGAAGC CTGGCCCCGA TGGTCTCCGT 
GGAATCCCTG GTCCTGTGGG TGAGCAAGGC CTCCCAGGAT CCCCAGGCCC CGATGGTCCA 
CCCGGCCCCA TGGGTCCTCC AGGACTCCCT GGCCTCAAAG GAGACTCCGG TCCCAAAGGT 
GAAAAGGGCC ATCCAGGCCT GATTGGACTC ATCGGCCCTC CGGGAGAGCA AGGTGAAAAG 
GGTGACCGTG GACTCCCAGG CCCCCAGGGT TCATCTGGTC CTAAAGGAGA TCAGGGCATC 
ACAGGTCCTT CTGGCCCACT TGGGCCTCCT GGTCCTCCTG GCTTGCCGGG CCCTCCAGGT 
CCCAAAGGTG CTAAGGGCTC TTCGGGTCCC ACCGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACTCCCCG GCCCACCTGG CCCTCCGGGT GAGGTCATCC AGCCCCTGCC AATCCAGGCC 
TCCAGGACTC GGCGGAACAT TGATGCCAGC CAGCTCCTGG ACGATGGGGC TGGGGAGAGC 
TACGTGGATT ATGCAGATGG CATGGAAGAG ATCTTTGGTT CCCTCAACTC CCTGAAGCTG 
GAGATTGAAC AGATGAAGCG ACCACTGGGC ACCCAGCAGA ACCCAGCCCG TACCTGCAAG 
GATCTACAGC TCTGTCATCC TGACTTCCCA GATGGCGAAT ACTGGGTCGA TCCCAACCAA 
GGGTGCTCCA GGGACTCCTT CAAAGTCTAC TGCAATTTCA CAGCTGGAGG GTCCACGTGC 
GTCTTCCCTG ACAAGAAGTC TGAGGGAAGT AAAATGGCCC GGTGGCCCAA AGAACAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGT TCCCTGCTCT CCTATGTGGA TGCTGAAGGC 
AACCCCGTGG GCGTGGTACA AATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACAACTGCTA CCAGTCCGTG GCCTGGCAGG ATGCCGCCAC AGGCAGCTAT 
GATAAGGCTA TCCGCTTCTT GGGCTCCAAC GATGAGGAAA TGTCTTATGA TAACAACCCC 
TACATCCGTG CCCTGGTGGA TGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTGCTG 
GAGATCGACA CGCCCAAAGT AGAGCAAGTC CCCATTGTGG ACATCATGTT CAACGACTTT 
GGCGAAGCCT CACAGAAATT TGGATTTGAA GTGGGGCCAG CTTGCTTCCT AGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

KLF13 is a genetic modifier of the Holt-Oram syndrome gene TBX5.
Human molecular genetics26(5)942-954(2017 03)
Darwich R,Li W,Yamak A,Komati H,Andelfinger G,Sun K,Nemer M

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

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Col5a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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