Col4a4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col4a4
Entrez Gene ID	12829
Full Name	collagen, type IV, alpha 4
Synonyms	E130010M05Rik,[a]4(IV)
General protein information	Preferred Names collagen, type IV, alpha 4 Names collagen alpha-4(IV) chain col4a4 procollagen, type IV, alpha 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C5

mRNA and Protein(s)

mRNA	Protein	Name
XM_011238652.1	XP_011236954.1	collagen alpha-4(IV) chain isoform X1
XM_011238653.3	XP_011236955.1	collagen alpha-4(IV) chain isoform X2
NM_007735.2	NP_031761.1	collagen alpha-4(IV) chain precursor
XM_011238653.2	XP_011236955.1	collagen alpha-4(IV) chain isoform X2
XM_030245430.1	XP_030101290.1	collagen alpha-4(IV) chain isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A4	XP_516128.2
	COL4A4	XP_001110249.1
Canis lupus familiaris (dog)	COL4A4	NP_001026988.1
Homo sapiens (human)	COL4A4	NP_000083.3
Mus musculus (house mouse)	Col4a4	NP_031761.1

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

PDGFR? regulates craniofacial development through homodimers and functional heterodimers with PDGFR?.
Fantauzzo KA, Soriano P
Genes & development30(21)2443-2458(2016 Nov)

Thyroid follicle development requires Smad1/5- and endothelial cell-dependent basement membrane assembly.
Villacorte M, Delmarcelle AS, Lernoux M, Bouquet M, Lemoine P, Bols?e J, Umans L, de Sousa Lopes SC, Van Der Smissen P, Sasaki T, Bommer G, Henriet P, Refetoff S, Lemaigre FP, Zwijsen A, Courtoy PJ, Pierreux CE
Development (Cambridge, England)143(11)1958-70(2016 06)

A mouse Col4a4 mutation causing Alport glomerulosclerosis with abnormal collagen ?3?4?5(IV) trimers.
Korstanje R, Caputo CR, Doty RA, Cook SA, Bronson RT, Davisson MT, Miner JH
Kidney international85(6)1461-8(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report novel mouse model of Alport syndrome with accumulation abnormal collagen alpha3alpha4alpha5(IV) trimers in the glomerular basement membrane.
Title: A mouse Col4a4 mutation causing Alport glomerulosclerosis with abnormal collagen ?3?4?5(IV) trimers.

the NC1 domain of {alpha}3 chain is critical for {alpha}3{alpha}4{alpha}5 type IV collagen network assembly
Title: Identification of the NC1 domain of {alpha}3 chain as critical for {alpha}3{alpha}4{alpha}5 type IV collagen network assembly.

Alpha3(IV), alpha4(IV), and alpha5(IV) chains form a complex, which is a heterotrimer, and a defect in complex formation might be one of the molecular mechanisms underlying the pathogenesis of Alport syndrome.
Title: Characterization of assembly of recombinant type IV collagen alpha3, alpha4, and alpha5 chains in transfected cell strains.

In kidney, when expressed onto Col4a3(-/-) background, human alpha3(IV) chain restored expression of and co-assembled with mouse alpha4 and alpha5(IV) chains at sites where human alpha3(IV) was expressed. All three chains required for network assembly.
Title: A human-mouse chimera of the alpha3alpha4alpha5(IV) collagen protomer rescues the renal phenotype in Col4a3-/- Alport mice.

Collagen alpha3(IV) nor alpha4(IV) were detected in the lens capsule 2 weeks postnatal.
Title: Collagen IV in the developing lens capsule.

The following Col4a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col4a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84995	XM_030245430.1 Latest version!	Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu67434	XM_011238653.3 Latest version!	Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu20943	NM_007735.2 Latest version!	Mus musculus collagen, type IV, alpha 4 (Col4a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu67433	XM_011238652.1 Latest version!	Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OMu67434	XM_011238653.2	Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col4a4 ( XM_030245430.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101290.1 Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu84995
Clone ID Related Accession (Same CDS sequence)	XM_030245430.1
Accession Version	XM_030245430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-4(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTGCT TTTTCAGATG GACCAAGTCC TTTGTCACAG CCCCCTGGTC ACTAATATTT 
ATCCTCTTTA CTATACAATA TGAATATGGG AGTGGCAAGA AGTATGGCGG CCCCTGCGGA 
GGAAGAAACT GCTCTGTCTG CCAGTGTTTT CCTGAGAAGG GATCTCGGGG TCACCCAGGA 
CCACTGGGAC CACAGGGACC AATAGGGCCC TTGGGACCCC TGGGACCTAT TGGGATTCCA 
GGAGAAAAAG GAGAGAGAGG AGACAGTGGC TCACCTGGAC CACCAGGAGA GAAAGGAGAC 
AAGGGTCCAA CTGGTGTCCC TGGATTTCCA GGTGTGGATG GTGTACCTGG TCACCCAGGG 
CCTCCTGGAC CCAGAGGCAA ACCTGGTGTG GACGGCTACA ATGGCTCACG TGGTGATCCG 
GGCTATCCAG GAGAAAGAGG AGCTCCAGGC CCAGGCGGCC CCCCTGGTCA GCCTGGGGAA 
AATGGAGAAA AAGGAAGATC TGTGTACATT ACTGGTGGCG TTAAAGGTAT TCAGGGAGAC 
CGTGGGGACC CAGGACCACC CGGCTTACCA GGATCTAGGG GTGCACAAGG GTCACCAGGG 
CCCATGGGGC ATGCAGGCGC ACCAGGGCTA GCAGGACCTA TAGGTCATCC TGGAAGCCCT 
GGGTTGAAGG GAAATCCTGC CACTGGACTG AAGGGACAAA GGGGAGAGCC GGGTGAAGTT 
GGCCAGCGTG GTCCTCCTGG GCCCACGCTG TTGGTACAGC CGCCTGATTT GAGTATCTAT 
AAAGGAGAAA AGGGTGTAAA AGGAATGCCT GGAATGATTG GTCCCCCAGG ACCTCCAGGT 
CGCAAGGGAG CGCCTGGTGT CGGGATCAAA GGAGAGAAAG GTATCCCTGG GTTCCCAGGA 
CCCCGGGGTG AGCCTGGTTC GCATGGGCCT CCAGGTTTTC CAGGATTCAA GGGGATACAA 
GGAGCAGCTG GAGAGCCTGG GCTGTTCGGA TTTCTTGGTC CAAAGGGGGA TCTGGGAGAT 
CGCGGGTACC CGGGACCACC AGGCATTTTG TTGACTCCAG CTCCACCACT CAAAGGTGTT 
CCAGGGGACC CAGGACCCCC TGGCTACTAT GGGGAGATTG GAGATGTTGG ATTACCAGGT 
CCTCCAGGGC CCCCAGGCAG ACCAGGGGAA ACTTGTCCAG GCATGATGGG ACCTCCTGGA 
CCACCAGGGG TTCCTGGTCC TCCAGGATTT CCAGGAGAAG CTGGGGTTCC TGGAAGACTC 
GACTGTGCCC CAGGAAAACC TGGGAAGCCA GGGCTACCCG GCCTGCCTGG CGCACCAGGA 
CCAGAGGGGC CCCCTGGATC AGATGTGATA TACTGTCGCC CTGGGTGCCC TGGGCCAATG 
GGTGAAAAGG GAAAAGTGGG TCCTCCAGGA AGAAGAGGAG CCAAAGGAGC AAAAGGAAAC 
AAGGGACTCT GTACCTGTCC ACCTGGTCCC ATGGGACCCC CTGGTCCCCC AGGACCTCCT 
GGAAGACAAG GAAGTAAAGG AGACTTAGGG CTCCCTGGTT GGCATGGAGA AAAGGGCGAT 
CCAGGTCAGC CTGGTGCTGA AGGACCGCCA GGGCCACCAG GAAGACCTGG TGCCATGGGG 
CCCCCTGGTC ACAAAGGGGA AAAGGGTGAC ATGGTCATAT CAAGAGTGAA AGGGCAAAAA 
GGAGAAAGAG GACTGGATGG GCCACCAGGA TTTCCGGGGC CACATGGACA AGATGGTGGG 
GATGGACGTC CTGGAGAAAG AGGGGATCCT GGGCCACGAG GGGACCATAA GGATGCAGCC 
CCGGGTGAGA GAGGGCTTCC CGGACTGCCA GGCCCTCCAG GAAGAACAGG ACCTGAGGGG 
CCTCCAGGCC TGGGATTTCC AGGCCCACCA GGACAGCGAG GCCTACCCGG AGAGCCCGGG 
CGTCCGGGCA CCAGAGGCTT TGATGGCACG AAGGGGCAGA AAGGTGACTC CATTCTGTGT 
AATGTAAGCT ACCCGGGAAA ACCAGGTCTG CCAGGTCTTG ATGGACCTCC AGGTCTGAAG 
GGGTTTCCAG GCCCCCCAGG GGCTCCTGGT ATGAGATGTC CAGATGGGCA GAAAGGCCAG 
CGGGGCAAAC CGGGAATGTC TGGGATTCCA GGTCCACCTG GTTTTCGTGG TGACATGGGA 
GATCCAGGCA TCAAAGGTGA AAAGGGGACC TCCCCTATTG GACCCCCAGG CCCACCTGGG 
TCTCCTGGAA AGGATGGCCA GAAAGGAATC CCAGGAGACC CTGCATTTGG TGACCCAGGA 
CCCCCTGGAG AGAGGGGTCT TCCAGGAGCA CCAGGCATGA AAGGACAGAA AGGTCACCCA 
GGGTGCCCAG GGGCTGGAGG CCCTCCTGGC ATCCCTGGAT CTCCAGGTCT CAAAGGTCCC 
AAAGGTAGAG AGGGAAGTCG TGGCTTTCCA GGGATCCCAG GATCCCCTGG CCATTCCTGT 
GAAAGAGGTG CTCCCGGGAT CCCAGGACAA CCGGGACTTC CTGGAACTCC AGGTGATCCA 
GGGGCCCCAG GTTGGAAAGG CCAGCCAGGA GACATGGGTC CCTCTGGACC AGCTGGGATG 
AAGGGCCTCC CTGGTCTCCC AGGCCTACCA GGAGCTGATG GTCTCCGAGG GCCTCCTGGG 
ATTCCAGGCC CCAATGGGGA AGATGGTCTA CCTGGTCTTC CAGGCCTGAA GGGACTCCCA 
GGGCTGCCTG GCTTCCCTGG TTTTCCAGGA GAGAGAGGAA AGCCAGGGCC AGATGGAGAA 
CCTGGAAGAA AGGGAGAGGT TGGAGAGAAA GGCTGGCCTG GCTTGAAGGG AGACCTGGGA 
GAGCGAGGCG CCAAAGGAGA CAGAGGACTT CCTGGTGATG CAGGAGAAGC AGTCACTTCC 
AGAAAGGGTG AACCTGGGGA TGCTGGGCCT CCAGGAGATG GTGGGTTCTC AGGAGAGAGA 
GGTGATAAAG GAAGCTCAGG GATGCGAGGG GGAAGAGGGG ATCCCGGAAG AGATGGACTA 
CCGGGACTCC ACAGAGGGCA GCCTGGGATA GATGGGCCTC CTGGGCCTCC TGGGCCCCCT 
GGCCCTCCAG GATCACCTGG ACTGAGAGGA GTCATTGGTT TCCCAGGATT TCCAGGTGAC 
CAGGGTGATC CAGGTTCTCC AGGACCCCCT GGATTCCCAG GAGATGATGG AGCAAGAGGA 
CCTAAAGGAT ACAAAGGTGA CCCTGCCAGT CAGTGTGGTC CACCTGGTCC AAAGGGTGAG 
CCAGGTAGTC CTGGATACCA AGGACGTACT GGAGTCCCCG GAGAGAAAGG CTTTCCTGGA 
GATGAAGGAC CCCGAGGACC ACCAGGCAGA CCTGGACAGC CTGGATCTTT TGGACCACCA 
GGGTGTCCAG GTGACCCAGG GATGCCCGGA CTCAAGGGAC ATCCAGGAGA AGTGGGAGAC 
CCTGGGCCAA GAGGTGATGC GGGAGATTTC GGGAGGCCAG GTCCTGCTGG AGTGAAAGCC 
CTTCTGCTTT TCCCATCTCA GCTTTTCAAG ACTCCTAGCC TTTCAACAGG CCGTGGGTCC 
GTGTGCATCC CTCCGAGAGC AATGCTGAAG GAAGGAGCAC TCTTCCTTTG GAAGTTTCCA 
GCACCCTGGA GCACTTACTG GCAAGGAAGT AACCTGACGG GAGTGATCGT TAGAACAAAT 
GGTCTCTTGA TACTTTCTCC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101290.1
CDS	537..4220
Translation	MRCFFRWTKSFVTAPWSLIFILFTIQYEYGSGKKYGGPCGGRNCSVCQCFPEKGSRGHPGPLGPQGPIGPLGPLGPIGIPGEKGERGDSGSPGPPGEKGDKGPTGVPGFPGVDGVPGHPGPPGPRGKPGVDGYNGSRGDPGYPGERGAPGPGGPPGQPGENGEKGRSVYITGGVKGIQGDRGDPGPPGLPGSRGAQGSPGPMGHAGAPGLAGPIGHPGSPGLKGNPATGLKGQRGEPGEVGQRGPPGPTLLVQPPDLSIYKGEKGVKGMPGMIGPPGPPGRKGAPGVGIKGEKGIPGFPGPRGEPGSHGPPGFPGFKGIQGAAGEPGLFGFLGPKGDLGDRGYPGPPGILLTPAPPLKGVPGDPGPPGYYGEIGDVGLPGPPGPPGRPGETCPGMMGPPGPPGVPGPPGFPGEAGVPGRLDCAPGKPGKPGLPGLPGAPGPEGPPGSDVIYCRPGCPGPMGEKGKVGPPGRRGAKGAKGNKGLCTCPPGPMGPPGPPGPPGRQGSKGDLGLPGWHGEKGDPGQPGAEGPPGPPGRPGAMGPPGHKGEKGDMVISRVKGQKGERGLDGPPGFPGPHGQDGGDGRPGERGDPGPRGDHKDAAPGERGLPGLPGPPGRTGPEGPPGLGFPGPPGQRGLPGEPGRPGTRGFDGTKGQKGDSILCNVSYPGKPGLPGLDGPPGLKGFPGPPGAPGMRCPDGQKGQRGKPGMSGIPGPPGFRGDMGDPGIKGEKGTSPIGPPGPPGSPGKDGQKGIPGDPAFGDPGPPGERGLPGAPGMKGQKGHPGCPGAGGPPGIPGSPGLKGPKGREGSRGFPGIPGSPGHSCERGAPGIPGQPGLPGTPGDPGAPGWKGQPGDMGPSGPAGMKGLPGLPGLPGADGLRGPPGIPGPNGEDGLPGLPGLKGLPGLPGFPGFPGERGKPGPDGEPGRKGEVGEKGWPGLKGDLGERGAKGDRGLPGDAGEAVTSRKGEPGDAGPPGDGGFSGERGDKGSSGMRGGRGDPGRDGLPGLHRGQPGIDGPPGPPGPPGPPGSPGLRGVIGFPGFPGDQGDPGSPGPPGFPGDDGARGPKGYKGDPASQCGPPGPKGEPGSPGYQGRTGVPGEKGFPGDEGPRGPPGRPGQPGSFGPPGCPGDPGMPGLKGHPGEVGDPGPRGDAGDFGRPGPAGVKALLLFPSQLFKTPSLSTGRGSVCIPPRAMLKEGALFLWKFPAPWSTYWQGSNLTGVIVRTNGLLILSP

Target ORF information:

RefSeq Version	XM_030245430.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245430.1

ORF Insert Sequence:

ATGAGGTGCT TTTTCAGATG GACCAAGTCC TTTGTCACAG CCCCCTGGTC ACTAATATTT 
ATCCTCTTTA CTATACAATA TGAATATGGG AGTGGCAAGA AGTATGGCGG CCCCTGCGGA 
GGAAGAAACT GCTCTGTCTG CCAGTGTTTT CCTGAGAAGG GATCTCGGGG TCACCCAGGA 
CCACTGGGAC CACAGGGACC AATAGGGCCC TTGGGACCCC TGGGACCTAT TGGGATTCCA 
GGAGAAAAAG GAGAGAGAGG AGACAGTGGC TCACCTGGAC CACCAGGAGA GAAAGGAGAC 
AAGGGTCCAA CTGGTGTCCC TGGATTTCCA GGTGTGGATG GTGTACCTGG TCACCCAGGG 
CCTCCTGGAC CCAGAGGCAA ACCTGGTGTG GACGGCTACA ATGGCTCACG TGGTGATCCG 
GGCTATCCAG GAGAAAGAGG AGCTCCAGGC CCAGGCGGCC CCCCTGGTCA GCCTGGGGAA 
AATGGAGAAA AAGGAAGATC TGTGTACATT ACTGGTGGCG TTAAAGGTAT TCAGGGAGAC 
CGTGGGGACC CAGGACCACC CGGCTTACCA GGATCTAGGG GTGCACAAGG GTCACCAGGG 
CCCATGGGGC ATGCAGGCGC ACCAGGGCTA GCAGGACCTA TAGGTCATCC TGGAAGCCCT 
GGGTTGAAGG GAAATCCTGC CACTGGACTG AAGGGACAAA GGGGAGAGCC GGGTGAAGTT 
GGCCAGCGTG GTCCTCCTGG GCCCACGCTG TTGGTACAGC CGCCTGATTT GAGTATCTAT 
AAAGGAGAAA AGGGTGTAAA AGGAATGCCT GGAATGATTG GTCCCCCAGG ACCTCCAGGT 
CGCAAGGGAG CGCCTGGTGT CGGGATCAAA GGAGAGAAAG GTATCCCTGG GTTCCCAGGA 
CCCCGGGGTG AGCCTGGTTC GCATGGGCCT CCAGGTTTTC CAGGATTCAA GGGGATACAA 
GGAGCAGCTG GAGAGCCTGG GCTGTTCGGA TTTCTTGGTC CAAAGGGGGA TCTGGGAGAT 
CGCGGGTACC CGGGACCACC AGGCATTTTG TTGACTCCAG CTCCACCACT CAAAGGTGTT 
CCAGGGGACC CAGGACCCCC TGGCTACTAT GGGGAGATTG GAGATGTTGG ATTACCAGGT 
CCTCCAGGGC CCCCAGGCAG ACCAGGGGAA ACTTGTCCAG GCATGATGGG ACCTCCTGGA 
CCACCAGGGG TTCCTGGTCC TCCAGGATTT CCAGGAGAAG CTGGGGTTCC TGGAAGACTC 
GACTGTGCCC CAGGAAAACC TGGGAAGCCA GGGCTACCCG GCCTGCCTGG CGCACCAGGA 
CCAGAGGGGC CCCCTGGATC AGATGTGATA TACTGTCGCC CTGGGTGCCC TGGGCCAATG 
GGTGAAAAGG GAAAAGTGGG TCCTCCAGGA AGAAGAGGAG CCAAAGGAGC AAAAGGAAAC 
AAGGGACTCT GTACCTGTCC ACCTGGTCCC ATGGGACCCC CTGGTCCCCC AGGACCTCCT 
GGAAGACAAG GAAGTAAAGG AGACTTAGGG CTCCCTGGTT GGCATGGAGA AAAGGGCGAT 
CCAGGTCAGC CTGGTGCTGA AGGACCGCCA GGGCCACCAG GAAGACCTGG TGCCATGGGG 
CCCCCTGGTC ACAAAGGGGA AAAGGGTGAC ATGGTCATAT CAAGAGTGAA AGGGCAAAAA 
GGAGAAAGAG GACTGGATGG GCCACCAGGA TTTCCGGGGC CACATGGACA AGATGGTGGG 
GATGGACGTC CTGGAGAAAG AGGGGATCCT GGGCCACGAG GGGACCATAA GGATGCAGCC 
CCGGGTGAGA GAGGGCTTCC CGGACTGCCA GGCCCTCCAG GAAGAACAGG ACCTGAGGGG 
CCTCCAGGCC TGGGATTTCC AGGCCCACCA GGACAGCGAG GCCTACCCGG AGAGCCCGGG 
CGTCCGGGCA CCAGAGGCTT TGATGGCACG AAGGGGCAGA AAGGTGACTC CATTCTGTGT 
AATGTAAGCT ACCCGGGAAA ACCAGGTCTG CCAGGTCTTG ATGGACCTCC AGGTCTGAAG 
GGGTTTCCAG GCCCCCCAGG GGCTCCTGGT ATGAGATGTC CAGATGGGCA GAAAGGCCAG 
CGGGGCAAAC CGGGAATGTC TGGGATTCCA GGTCCACCTG GTTTTCGTGG TGACATGGGA 
GATCCAGGCA TCAAAGGTGA AAAGGGGACC TCCCCTATTG GACCCCCAGG CCCACCTGGG 
TCTCCTGGAA AGGATGGCCA GAAAGGAATC CCAGGAGACC CTGCATTTGG TGACCCAGGA 
CCCCCTGGAG AGAGGGGTCT TCCAGGAGCA CCAGGCATGA AAGGACAGAA AGGTCACCCA 
GGGTGCCCAG GGGCTGGAGG CCCTCCTGGC ATCCCTGGAT CTCCAGGTCT CAAAGGTCCC 
AAAGGTAGAG AGGGAAGTCG TGGCTTTCCA GGGATCCCAG GATCCCCTGG CCATTCCTGT 
GAAAGAGGTG CTCCCGGGAT CCCAGGACAA CCGGGACTTC CTGGAACTCC AGGTGATCCA 
GGGGCCCCAG GTTGGAAAGG CCAGCCAGGA GACATGGGTC CCTCTGGACC AGCTGGGATG 
AAGGGCCTCC CTGGTCTCCC AGGCCTACCA GGAGCTGATG GTCTCCGAGG GCCTCCTGGG 
ATTCCAGGCC CCAATGGGGA AGATGGTCTA CCTGGTCTTC CAGGCCTGAA GGGACTCCCA 
GGGCTGCCTG GCTTCCCTGG TTTTCCAGGA GAGAGAGGAA AGCCAGGGCC AGATGGAGAA 
CCTGGAAGAA AGGGAGAGGT TGGAGAGAAA GGCTGGCCTG GCTTGAAGGG AGACCTGGGA 
GAGCGAGGCG CCAAAGGAGA CAGAGGACTT CCTGGTGATG CAGGAGAAGC AGTCACTTCC 
AGAAAGGGTG AACCTGGGGA TGCTGGGCCT CCAGGAGATG GTGGGTTCTC AGGAGAGAGA 
GGTGATAAAG GAAGCTCAGG GATGCGAGGG GGAAGAGGGG ATCCCGGAAG AGATGGACTA 
CCGGGACTCC ACAGAGGGCA GCCTGGGATA GATGGGCCTC CTGGGCCTCC TGGGCCCCCT 
GGCCCTCCAG GATCACCTGG ACTGAGAGGA GTCATTGGTT TCCCAGGATT TCCAGGTGAC 
CAGGGTGATC CAGGTTCTCC AGGACCCCCT GGATTCCCAG GAGATGATGG AGCAAGAGGA 
CCTAAAGGAT ACAAAGGTGA CCCTGCCAGT CAGTGTGGTC CACCTGGTCC AAAGGGTGAG 
CCAGGTAGTC CTGGATACCA AGGACGTACT GGAGTCCCCG GAGAGAAAGG CTTTCCTGGA 
GATGAAGGAC CCCGAGGACC ACCAGGCAGA CCTGGACAGC CTGGATCTTT TGGACCACCA 
GGGTGTCCAG GTGACCCAGG GATGCCCGGA CTCAAGGGAC ATCCAGGAGA AGTGGGAGAC 
CCTGGGCCAA GAGGTGATGC GGGAGATTTC GGGAGGCCAG GTCCTGCTGG AGTGAAAGCC 
CTTCTGCTTT TCCCATCTCA GCTTTTCAAG ACTCCTAGCC TTTCAACAGG CCGTGGGTCC 
GTGTGCATCC CTCCGAGAGC AATGCTGAAG GAAGGAGCAC TCTTCCTTTG GAAGTTTCCA 
GCACCCTGGA GCACTTACTG GCAAGGAAGT AACCTGACGG GAGTGATCGT TAGAACAAAT 
GGTCTCTTGA TACTTTCTCC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a4 ( XM_011238653.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236955.1 Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu67434
Clone ID Related Accession (Same CDS sequence)	XM_011238653.3 , XM_011238653.2
Accession Version	XM_011238653.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-4(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238653.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTGCT TTTTCAGATG GACCAAGTCC TTTGTCACAG CCCCCTGGTC ACTAATATTT 
ATCCTCTTTA CTATACAATA TGAATATGGG AGTGGCAAGA AGTATGGCGG CCCCTGCGGA 
GGAAGAAACT GCTCTGTCTG CCAGTGTTTT CCTGAGAAGG GATCTCGGGG TCACCCAGGA 
CCACTGGGAC CACAGGGACC AATAGGGCCC TTGGGACCCC TGGGACCTAT TGGGATTCCA 
GGAGAAAAAG GAGAGAGAGG AGACAGTGGC TCACCTGGAC CACCAGGAGA GAAAGGAGAC 
AAGGGTCCAA CTGGTGTCCC TGGATTTCCA GGTGTGGATG GTGTACCTGG TCACCCAGGG 
CCTCCTGGAC CCAGAGGCAA ACCTGGTGTG GACGGCTACA ATGGCTCACG TGGTGATCCG 
GGCTATCCAG GAGAAAGAGG AGCTCCAGGC CCAGGCGGCC CCCCTGGTCA GCCTGGGGAA 
AATGGAGAAA AAGGAAGATC TGTGTACATT ACTGGTGGCG TTAAAGGTAT TCAGGGAGAC 
CGTGGGGACC CAGGACCACC CGGCTTACCA GGATCTAGGG GTGCACAAGG GTCACCAGGG 
CCCATGGGGC ATGCAGGCGC ACCAGGGCTA GCAGGACCTA TAGGTCATCC TGGAAGCCCT 
GGGTTGAAGG GAAATCCTGC CACTGGACTG AAGGGACAAA GGGGAGAGCC GGGTGAAGTT 
GGCCAGCGTG GTCCTCCTGG GCCCACGCTG TTGGTACAGC CGCCTGATTT GAGTATCTAT 
AAAGGAGAAA AGGGTGTAAA AGGAATGCCT GGAATGATTG GTCCCCCAGG ACCTCCAGGT 
CGCAAGGGAG CGCCTGGTGT CGGGATCAAA GGAGAGAAAG GTATCCCTGG GTTCCCAGGA 
CCCCGGGGTG AGCCTGGTTC GCATGGGCCT CCAGGTTTTC CAGGATTCAA GGGGATACAA 
GGAGCAGCTG GAGAGCCTGG GCTGTTCGGA TTTCTTGGTC CAAAGGGGGA TCTGGGAGAT 
CGCGGGTACC CGGGACCACC AGGCATTTTG TTGACTCCAG CTCCACCACT CAAAGGTGTT 
CCAGGGGACC CAGGACCCCC TGGCTACTAT GGGGAGATTG GAGATGTTGG ATTACCAGGT 
CCTCCAGGGC CCCCAGGCAG ACCAGGGGAA ACTTGTCCAG GCATGATGGG ACCTCCTGGA 
CCACCAGGGG TTCCTGGTCC TCCAGGATTT CCAGGAGAAG CTGGGGTTCC TGGAAGACTC 
GACTGTGCCC CAGGAAAACC TGGGAAGCCA GGGCTACCCG GCCTGCCTGG CGCACCAGGA 
CCAGAGGGGC CCCCTGGATC AGATGTGATA TACTGTCGCC CTGGGTGCCC TGGGCCAATG 
GGTGAAAAGG GAAAAGTGGG TCCTCCAGGA AGAAGAGGAG CCAAAGGAGC AAAAGGAAAC 
AAGGGACTCT GTACCTGTCC ACCTGGTCCC ATGGGACCCC CTGGTCCCCC AGGACCTCCT 
GGAAGACAAG GAAGTAAAGG AGACTTAGGG CTCCCTGGTT GGCATGGAGA AAAGGGCGAT 
CCAGGTCAGC CTGGTGCTGA AGGACCGCCA GGGCCACCAG GAAGACCTGG TGCCATGGGG 
CCCCCTGGTC ACAAAGGGGA AAAGGGTGAC ATGGTCATAT CAAGAGTGAA AGGGCAAAAA 
GGAGAAAGAG GACTGGATGG GCCACCAGGA TTTCCGGGGC CACATGGACA AGATGGTGGG 
GATGGACGTC CTGGAGAAAG AGGGGATCCT GGGCCACGAG GGGACCATAA GGATGCAGCC 
CCGGGTGAGA GAGGGCTTCC CGGACTGCCA GGCCCTCCAG GAAGAACAGG ACCTGAGGGG 
CCTCCAGGCC TGGGATTTCC AGGCCCACCA GGACAGCGAG GCCTACCCGG AGAGCCCGGG 
CGTCCGGGCA CCAGAGGCTT TGATGGCACG AAGGGGCAGA AAGGTGACTC CATTCTGTGT 
AATGTAAGCT ACCCGGGAAA ACCAGGTCTG CCAGGTCTTG ATGGACCTCC AGGTCTGAAG 
GGGTTTCCAG GCCCCCCAGG GGCTCCTGGT ATGAGATGTC CAGATGGGCA GAAAGGCCAG 
CGGGGCAAAC CGGGAATGTC TGGGATTCCA GGTCCACCTG GTTTTCGTGG TGACATGGGA 
GATCCAGGCA TCAAAGGTGA AAAGGGGACC TCCCCTATTG GACCCCCAGG CCCACCTGGG 
TCTCCTGGAA AGGATGGCCA GAAAGGAATC CCAGGAGACC CTGCATTTGG TGACCCAGGA 
CCCCCTGGAG AGAGGGGTCT TCCAGGAGCA CCAGGCATGA AAGGACAGAA AGGTCACCCA 
GGGTGCCCAG GGGCTGGAGG CCCTCCTGGC ATCCCTGGAT CTCCAGGTCT CAAAGGTCCC 
AAAGGTAGAG AGGGAAGTCG TGGCTTTCCA GGGATCCCAG GATCCCCTGG CCATTCCTGT 
GAAAGAGGTG CTCCCGGGAT CCCAGGACAA CCGGGACTTC CTGGAACTCC AGGTGATCCA 
GGGGCCCCAG GTTGGAAAGG CCAGCCAGGA GACATGGGTC CCTCTGGACC AGCTGGGATG 
AAGGGCCTCC CTGGTCTCCC AGGCCTACCA GGAGCTGATG GTCTCCGAGG GCCTCCTGGG 
ATTCCAGGCC CCAATGGGGA AGATGGTCTA CCTGGTCTTC CAGGCCTGAA GGGACTCCCA 
GGGCTGCCTG GCTTCCCTGG TTTTCCAGGA GAGAGAGGAA AGCCAGGGCC AGATGGAGAA 
CCTGGAAGAA AGGGAGAGGT TGGAGAGAAA GGCTGGCCTG GCTTGAAGGG AGACCTGGGA 
GAGCGAGGCG CCAAAGGAGA CAGAGGACTT CCTGGTGATG CAGGAGAAGC AGTCACTTCC 
AGAAAGGGTG AACCTGGGGA TGCTGGGCCT CCAGGAGATG GTGGGTTCTC AGGAGAGAGA 
GGTGATAAAG GAAGCTCAGG GATGCGAGGG GGAAGAGGGG ATCCCGGAAG AGATGGACTA 
CCGGGACTCC ACAGAGGGCA GCCTGGGATA GATGGGCCTC CTGGGCCTCC TGGGCCCCCT 
GGCCCTCCAG GATCACCTGG ACTGAGAGGA GTCATTGGTT TCCCAGGATT TCCAGGTGAC 
CAGGGTGATC CAGGTTCTCC AGGACCCCCT GGATTCCCAG GAGATGATGG AGCAAGAGGA 
CCTAAAGGAT ACAAAGGTGA CCCTGCCAGT CAGTGTGGTC CACCTGGTCC AAAGGGTGAG 
CCAGGTAGTC CTGGATACCA AGGACGTACT GGAGTCCCCG GAGAGAAAGG CTTTCCTGGA 
GATGAAGGAC CCCGAGGACC ACCAGGCAGA CCTGGACAGC CTGGATCTTT TGGACCACCA 
GGGTGTCCAG GTGACCCAGG GATGCCCGGA CTCAAGGGAC ATCCAGGAGA AGTGGGAGAC 
CCTGGGCCAA GAGGTGATGC GGGAGATTTC GGGAGGCCAG GTCCTGCTGG AGTGAAAGGG 
CCTCTCGGGT CTCCTGGTCT GAATGGCTTA CATGGTCTGA AGGGTGAGAA AGGAACAAAA 
GGCGCCTCAG GTTTGCTCGA AATGGGTCCA CCTGGGCCAA TGGGGATGCC TGGGCAAAAA 
GGAGAGAAAG GAGACCCTGG GAGTCCAGGG ATTTCTCCTC CAGGTCTTCC TGGAGAAAAG 
GGCTTCCCAG GACCCCCAGG GAGACCAGGA CCACCTGGTC CCGCAGGTGC CCCAGGAAGA 
GCTGCTAAAG GTGACATTCC TGATCCAGGT CCACCTGGAG ACCGGGGACC TCCTGGCCCC 
GATGGCCCAA GAGGAGTACC TGGGCCTCCG GGTTCCCCTG GGAATGTTGA CCTTCTGAAA 
GGGGACCCAG GTGACTGTGG TTTGCCAGGA CCACCTGGCT CCCGAGGCCC ACCAGGCCCT 
CCAGGGTGTC AGGGTCCCCC AGGATGTGAT GGCAAAGATG GCCAGAAAGG ACCAATGGGA 
CTTCCAGGGT TGCCAGGGCC ACCTGGTCTT CCTGGGGCAC CTGGCGAGAA GGGATTACCT 
GGCCCTCCAG GCAGAAAAGG GCCAGTAGGT CCTCCAGGCT GCAGAGGTGA ACCTGGGCCT 
CCTGCAGACG TGGATTCCTG TCCTCGAATC CCAGGACTTC CTGGAGTGCC AGGTCCCAGA 
GGACCAGAAG GAGCTATGGG GGAACCTGGA CGGAGAGGCC TGCCTGGACC AGGGTGCAAA 
GGAGAGCCTG GACCGGATGG CAGACGGGGC CAGGATGGCA TCCCTGGATC TCCCGGGCCT 
CCTGGACGCA AAGGTGACAC TGGAGAGGCA GGGTGTCCTG GAGCACCAGG TTTCTTACTG 
AGTCTGGAGA TTGCCAACTG GCAAGGCTGC CCCAGCAAGG AGCTGCAAGC ATCCTCCCGT 
CTCCATGCCG CCAACACTGG GTTCTGGATT CAGTGGGCTT TATTGGGGTT CCTGGGGATC 
TGGACTCCTT GTTTTACCGA AAGTGATACC AGCCCTATGG GGGTCATTGG TATACTGCAT 
CATTTAGGGA AACTGTCAGG GAACGGAGAC TACATGCTTA ATTCAGAAGC AATTAAAAAT 
TATATATTTT TCAGTTCTCA GTTGAGTGAT CAGCCTTGCA GAAGCACAGA GGCTTTGCTG 
GAGGAGTTGT GTTTTTGTGT ACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236955.1
CDS	537..5183
Translation	MRCFFRWTKSFVTAPWSLIFILFTIQYEYGSGKKYGGPCGGRNCSVCQCFPEKGSRGHPGPLGPQGPIGPLGPLGPIGIPGEKGERGDSGSPGPPGEKGDKGPTGVPGFPGVDGVPGHPGPPGPRGKPGVDGYNGSRGDPGYPGERGAPGPGGPPGQPGENGEKGRSVYITGGVKGIQGDRGDPGPPGLPGSRGAQGSPGPMGHAGAPGLAGPIGHPGSPGLKGNPATGLKGQRGEPGEVGQRGPPGPTLLVQPPDLSIYKGEKGVKGMPGMIGPPGPPGRKGAPGVGIKGEKGIPGFPGPRGEPGSHGPPGFPGFKGIQGAAGEPGLFGFLGPKGDLGDRGYPGPPGILLTPAPPLKGVPGDPGPPGYYGEIGDVGLPGPPGPPGRPGETCPGMMGPPGPPGVPGPPGFPGEAGVPGRLDCAPGKPGKPGLPGLPGAPGPEGPPGSDVIYCRPGCPGPMGEKGKVGPPGRRGAKGAKGNKGLCTCPPGPMGPPGPPGPPGRQGSKGDLGLPGWHGEKGDPGQPGAEGPPGPPGRPGAMGPPGHKGEKGDMVISRVKGQKGERGLDGPPGFPGPHGQDGGDGRPGERGDPGPRGDHKDAAPGERGLPGLPGPPGRTGPEGPPGLGFPGPPGQRGLPGEPGRPGTRGFDGTKGQKGDSILCNVSYPGKPGLPGLDGPPGLKGFPGPPGAPGMRCPDGQKGQRGKPGMSGIPGPPGFRGDMGDPGIKGEKGTSPIGPPGPPGSPGKDGQKGIPGDPAFGDPGPPGERGLPGAPGMKGQKGHPGCPGAGGPPGIPGSPGLKGPKGREGSRGFPGIPGSPGHSCERGAPGIPGQPGLPGTPGDPGAPGWKGQPGDMGPSGPAGMKGLPGLPGLPGADGLRGPPGIPGPNGEDGLPGLPGLKGLPGLPGFPGFPGERGKPGPDGEPGRKGEVGEKGWPGLKGDLGERGAKGDRGLPGDAGEAVTSRKGEPGDAGPPGDGGFSGERGDKGSSGMRGGRGDPGRDGLPGLHRGQPGIDGPPGPPGPPGPPGSPGLRGVIGFPGFPGDQGDPGSPGPPGFPGDDGARGPKGYKGDPASQCGPPGPKGEPGSPGYQGRTGVPGEKGFPGDEGPRGPPGRPGQPGSFGPPGCPGDPGMPGLKGHPGEVGDPGPRGDAGDFGRPGPAGVKGPLGSPGLNGLHGLKGEKGTKGASGLLEMGPPGPMGMPGQKGEKGDPGSPGISPPGLPGEKGFPGPPGRPGPPGPAGAPGRAAKGDIPDPGPPGDRGPPGPDGPRGVPGPPGSPGNVDLLKGDPGDCGLPGPPGSRGPPGPPGCQGPPGCDGKDGQKGPMGLPGLPGPPGLPGAPGEKGLPGPPGRKGPVGPPGCRGEPGPPADVDSCPRIPGLPGVPGPRGPEGAMGEPGRRGLPGPGCKGEPGPDGRRGQDGIPGSPGPPGRKGDTGEAGCPGAPGFLLSLEIANWQGCPSKELQASSRLHAANTGFWIQWALLGFLGIWTPCFTESDTSPMGVIGILHHLGKLSGNGDYMLNSEAIKNYIFFSSQLSDQPCRSTEALLEELCFCVL

Target ORF information:

RefSeq Version	XM_011238653.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238653.3

ORF Insert Sequence:

ATGAGGTGCT TTTTCAGATG GACCAAGTCC TTTGTCACAG CCCCCTGGTC ACTAATATTT 
ATCCTCTTTA CTATACAATA TGAATATGGG AGTGGCAAGA AGTATGGCGG CCCCTGCGGA 
GGAAGAAACT GCTCTGTCTG CCAGTGTTTT CCTGAGAAGG GATCTCGGGG TCACCCAGGA 
CCACTGGGAC CACAGGGACC AATAGGGCCC TTGGGACCCC TGGGACCTAT TGGGATTCCA 
GGAGAAAAAG GAGAGAGAGG AGACAGTGGC TCACCTGGAC CACCAGGAGA GAAAGGAGAC 
AAGGGTCCAA CTGGTGTCCC TGGATTTCCA GGTGTGGATG GTGTACCTGG TCACCCAGGG 
CCTCCTGGAC CCAGAGGCAA ACCTGGTGTG GACGGCTACA ATGGCTCACG TGGTGATCCG 
GGCTATCCAG GAGAAAGAGG AGCTCCAGGC CCAGGCGGCC CCCCTGGTCA GCCTGGGGAA 
AATGGAGAAA AAGGAAGATC TGTGTACATT ACTGGTGGCG TTAAAGGTAT TCAGGGAGAC 
CGTGGGGACC CAGGACCACC CGGCTTACCA GGATCTAGGG GTGCACAAGG GTCACCAGGG 
CCCATGGGGC ATGCAGGCGC ACCAGGGCTA GCAGGACCTA TAGGTCATCC TGGAAGCCCT 
GGGTTGAAGG GAAATCCTGC CACTGGACTG AAGGGACAAA GGGGAGAGCC GGGTGAAGTT 
GGCCAGCGTG GTCCTCCTGG GCCCACGCTG TTGGTACAGC CGCCTGATTT GAGTATCTAT 
AAAGGAGAAA AGGGTGTAAA AGGAATGCCT GGAATGATTG GTCCCCCAGG ACCTCCAGGT 
CGCAAGGGAG CGCCTGGTGT CGGGATCAAA GGAGAGAAAG GTATCCCTGG GTTCCCAGGA 
CCCCGGGGTG AGCCTGGTTC GCATGGGCCT CCAGGTTTTC CAGGATTCAA GGGGATACAA 
GGAGCAGCTG GAGAGCCTGG GCTGTTCGGA TTTCTTGGTC CAAAGGGGGA TCTGGGAGAT 
CGCGGGTACC CGGGACCACC AGGCATTTTG TTGACTCCAG CTCCACCACT CAAAGGTGTT 
CCAGGGGACC CAGGACCCCC TGGCTACTAT GGGGAGATTG GAGATGTTGG ATTACCAGGT 
CCTCCAGGGC CCCCAGGCAG ACCAGGGGAA ACTTGTCCAG GCATGATGGG ACCTCCTGGA 
CCACCAGGGG TTCCTGGTCC TCCAGGATTT CCAGGAGAAG CTGGGGTTCC TGGAAGACTC 
GACTGTGCCC CAGGAAAACC TGGGAAGCCA GGGCTACCCG GCCTGCCTGG CGCACCAGGA 
CCAGAGGGGC CCCCTGGATC AGATGTGATA TACTGTCGCC CTGGGTGCCC TGGGCCAATG 
GGTGAAAAGG GAAAAGTGGG TCCTCCAGGA AGAAGAGGAG CCAAAGGAGC AAAAGGAAAC 
AAGGGACTCT GTACCTGTCC ACCTGGTCCC ATGGGACCCC CTGGTCCCCC AGGACCTCCT 
GGAAGACAAG GAAGTAAAGG AGACTTAGGG CTCCCTGGTT GGCATGGAGA AAAGGGCGAT 
CCAGGTCAGC CTGGTGCTGA AGGACCGCCA GGGCCACCAG GAAGACCTGG TGCCATGGGG 
CCCCCTGGTC ACAAAGGGGA AAAGGGTGAC ATGGTCATAT CAAGAGTGAA AGGGCAAAAA 
GGAGAAAGAG GACTGGATGG GCCACCAGGA TTTCCGGGGC CACATGGACA AGATGGTGGG 
GATGGACGTC CTGGAGAAAG AGGGGATCCT GGGCCACGAG GGGACCATAA GGATGCAGCC 
CCGGGTGAGA GAGGGCTTCC CGGACTGCCA GGCCCTCCAG GAAGAACAGG ACCTGAGGGG 
CCTCCAGGCC TGGGATTTCC AGGCCCACCA GGACAGCGAG GCCTACCCGG AGAGCCCGGG 
CGTCCGGGCA CCAGAGGCTT TGATGGCACG AAGGGGCAGA AAGGTGACTC CATTCTGTGT 
AATGTAAGCT ACCCGGGAAA ACCAGGTCTG CCAGGTCTTG ATGGACCTCC AGGTCTGAAG 
GGGTTTCCAG GCCCCCCAGG GGCTCCTGGT ATGAGATGTC CAGATGGGCA GAAAGGCCAG 
CGGGGCAAAC CGGGAATGTC TGGGATTCCA GGTCCACCTG GTTTTCGTGG TGACATGGGA 
GATCCAGGCA TCAAAGGTGA AAAGGGGACC TCCCCTATTG GACCCCCAGG CCCACCTGGG 
TCTCCTGGAA AGGATGGCCA GAAAGGAATC CCAGGAGACC CTGCATTTGG TGACCCAGGA 
CCCCCTGGAG AGAGGGGTCT TCCAGGAGCA CCAGGCATGA AAGGACAGAA AGGTCACCCA 
GGGTGCCCAG GGGCTGGAGG CCCTCCTGGC ATCCCTGGAT CTCCAGGTCT CAAAGGTCCC 
AAAGGTAGAG AGGGAAGTCG TGGCTTTCCA GGGATCCCAG GATCCCCTGG CCATTCCTGT 
GAAAGAGGTG CTCCCGGGAT CCCAGGACAA CCGGGACTTC CTGGAACTCC AGGTGATCCA 
GGGGCCCCAG GTTGGAAAGG CCAGCCAGGA GACATGGGTC CCTCTGGACC AGCTGGGATG 
AAGGGCCTCC CTGGTCTCCC AGGCCTACCA GGAGCTGATG GTCTCCGAGG GCCTCCTGGG 
ATTCCAGGCC CCAATGGGGA AGATGGTCTA CCTGGTCTTC CAGGCCTGAA GGGACTCCCA 
GGGCTGCCTG GCTTCCCTGG TTTTCCAGGA GAGAGAGGAA AGCCAGGGCC AGATGGAGAA 
CCTGGAAGAA AGGGAGAGGT TGGAGAGAAA GGCTGGCCTG GCTTGAAGGG AGACCTGGGA 
GAGCGAGGCG CCAAAGGAGA CAGAGGACTT CCTGGTGATG CAGGAGAAGC AGTCACTTCC 
AGAAAGGGTG AACCTGGGGA TGCTGGGCCT CCAGGAGATG GTGGGTTCTC AGGAGAGAGA 
GGTGATAAAG GAAGCTCAGG GATGCGAGGG GGAAGAGGGG ATCCCGGAAG AGATGGACTA 
CCGGGACTCC ACAGAGGGCA GCCTGGGATA GATGGGCCTC CTGGGCCTCC TGGGCCCCCT 
GGCCCTCCAG GATCACCTGG ACTGAGAGGA GTCATTGGTT TCCCAGGATT TCCAGGTGAC 
CAGGGTGATC CAGGTTCTCC AGGACCCCCT GGATTCCCAG GAGATGATGG AGCAAGAGGA 
CCTAAAGGAT ACAAAGGTGA CCCTGCCAGT CAGTGTGGTC CACCTGGTCC AAAGGGTGAG 
CCAGGTAGTC CTGGATACCA AGGACGTACT GGAGTCCCCG GAGAGAAAGG CTTTCCTGGA 
GATGAAGGAC CCCGAGGACC ACCAGGCAGA CCTGGACAGC CTGGATCTTT TGGACCACCA 
GGGTGTCCAG GTGACCCAGG GATGCCCGGA CTCAAGGGAC ATCCAGGAGA AGTGGGAGAC 
CCTGGGCCAA GAGGTGATGC GGGAGATTTC GGGAGGCCAG GTCCTGCTGG AGTGAAAGGG 
CCTCTCGGGT CTCCTGGTCT GAATGGCTTA CATGGTCTGA AGGGTGAGAA AGGAACAAAA 
GGCGCCTCAG GTTTGCTCGA AATGGGTCCA CCTGGGCCAA TGGGGATGCC TGGGCAAAAA 
GGAGAGAAAG GAGACCCTGG GAGTCCAGGG ATTTCTCCTC CAGGTCTTCC TGGAGAAAAG 
GGCTTCCCAG GACCCCCAGG GAGACCAGGA CCACCTGGTC CCGCAGGTGC CCCAGGAAGA 
GCTGCTAAAG GTGACATTCC TGATCCAGGT CCACCTGGAG ACCGGGGACC TCCTGGCCCC 
GATGGCCCAA GAGGAGTACC TGGGCCTCCG GGTTCCCCTG GGAATGTTGA CCTTCTGAAA 
GGGGACCCAG GTGACTGTGG TTTGCCAGGA CCACCTGGCT CCCGAGGCCC ACCAGGCCCT 
CCAGGGTGTC AGGGTCCCCC AGGATGTGAT GGCAAAGATG GCCAGAAAGG ACCAATGGGA 
CTTCCAGGGT TGCCAGGGCC ACCTGGTCTT CCTGGGGCAC CTGGCGAGAA GGGATTACCT 
GGCCCTCCAG GCAGAAAAGG GCCAGTAGGT CCTCCAGGCT GCAGAGGTGA ACCTGGGCCT 
CCTGCAGACG TGGATTCCTG TCCTCGAATC CCAGGACTTC CTGGAGTGCC AGGTCCCAGA 
GGACCAGAAG GAGCTATGGG GGAACCTGGA CGGAGAGGCC TGCCTGGACC AGGGTGCAAA 
GGAGAGCCTG GACCGGATGG CAGACGGGGC CAGGATGGCA TCCCTGGATC TCCCGGGCCT 
CCTGGACGCA AAGGTGACAC TGGAGAGGCA GGGTGTCCTG GAGCACCAGG TTTCTTACTG 
AGTCTGGAGA TTGCCAACTG GCAAGGCTGC CCCAGCAAGG AGCTGCAAGC ATCCTCCCGT 
CTCCATGCCG CCAACACTGG GTTCTGGATT CAGTGGGCTT TATTGGGGTT CCTGGGGATC 
TGGACTCCTT GTTTTACCGA AAGTGATACC AGCCCTATGG GGGTCATTGG TATACTGCAT 
CATTTAGGGA AACTGTCAGG GAACGGAGAC TACATGCTTA ATTCAGAAGC AATTAAAAAT 
TATATATTTT TCAGTTCTCA GTTGAGTGAT CAGCCTTGCA GAAGCACAGA GGCTTTGCTG 
GAGGAGTTGT GTTTTTGTGT ACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a4 ( NM_007735.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031761.1 Mus musculus collagen, type IV, alpha 4 (Col4a4), mRNA.

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CloneID	OMu20943
Clone ID Related Accession (Same CDS sequence)	NM_007735.2
Accession Version	NM_007735.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	collagen alpha-4(IV) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK164732.1, AF169388.1 and AC160543.13. On Apr 25, 2007 this sequence version replaced NM_007735.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF169388.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGGTGCT TTTTCAGATG GACCAAGTCC TTTGTCACAG CCCCCTGGTC ACTAATATTT 
ATCCTCTTTA CTATACAATA TGAATATGGG AGTGGCAAGA AGTATGGCGG CCCCTGCGGA 
GGAAGAAACT GCTCTGTCTG CCAGTGTTTT CCTGAGAAGG GATCTCGGGG TCACCCAGGA 
CCACTGGGAC CACAGGGACC AATAGGGCCC TTGGGACCCC TGGGACCTAT TGGGATTCCA 
GGAGAAAAAG GAGAGAGAGG AGACAGTGGC TCACCTGGAC CACCAGGAGA GAAAGGAGAC 
AAGGGTCCAA CTGGTGTCCC TGGATTTCCA GGTGTGGATG GTGTACCTGG TCACCCAGGG 
CCTCCTGGAC CCAGAGGCAA ACCTGGTGTG GACGGCTACA ATGGCTCACG TGGTGATCCG 
GGCTATCCAG GAGAAAGAGG AGCTCCAGGC CCAGGCGGCC CCCCTGGTCA GCCTGGGGAA 
AATGGAGAAA AAGGAAGATC TGTGTACATT ACTGGTGGCG TTAAAGGTAT TCAGGGAGAC 
CGTGGGGACC CAGGACCACC CGGCTTACCA GGATCTAGGG GTGCACAAGG GTCACCAGGG 
CCCATGGGGC ATGCAGGCGC ACCAGGGCTA GCAGGACCTA TAGGTCATCC TGGAAGCCCT 
GGGTTGAAGG GAAATCCTGC CACTGGACTG AAGGGACAAA GGGGAGAGCC GGGTGAAGTT 
GGCCAGCGTG GTCCTCCTGG GCCCACGCTG TTGGTACAGC CGCCTGATTT GAGTATCTAT 
AAAGGAGAAA AGGGTGTAAA AGGAATGCCT GGAATGATTG GTCCCCCAGG ACCTCCAGGT 
CGCAAGGGAG CGCCTGGTGT CGGGATCAAA GGAGAGAAAG GTATCCCTGG GTTCCCAGGA 
CCCCGGGGTG AGCCTGGTTC GCATGGGCCT CCAGGTTTTC CAGGATTCAA GGGGATACAA 
GGAGCAGCTG GAGAGCCTGG GCTGTTCGGA TTTCTTGGTC CAAAGGGGGA TCTGGGAGAT 
CGCGGGTACC CGGGACCACC AGGCATTTTG TTGACTCCAG CTCCACCACT CAAAGGTGTT 
CCAGGGGACC CAGGACCCCC TGGCTACTAT GGGGAGATTG GAGATGTTGG ATTACCAGGT 
CCTCCAGGGC CCCCAGGCAG ACCAGGGGAA ACTTGTCCAG GCATGATGGG ACCTCCTGGA 
CCACCAGGGG TTCCTGGTCC TCCAGGATTT CCAGGAGAAG CTGGGGTTCC TGGAAGACTC 
GACTGTGCCC CAGGAAAACC TGGGAAGCCA GGGCTACCCG GCCTGCCTGG CGCACCAGGA 
CCAGAGGGGC CCCCTGGATC AGATGTGATA TACTGTCGCC CTGGGTGCCC TGGGCCAATG 
GGTGAAAAGG GAAAAGTGGG TCCTCCAGGA AGAAGAGGAG CCAAAGGAGC AAAAGGAAAC 
AAGGGACTCT GTACCTGTCC ACCTGGTCCC ATGGGACCCC CTGGTCCCCC AGGACCTCCT 
GGAAGACAAG GAAGTAAAGG AGACTTAGGG CTCCCTGGTT GGCATGGAGA AAAGGGCGAT 
CCAGGTCAGC CTGGTGCTGA AGGACCGCCA GGGCCACCAG GAAGACCTGG TGCCATGGGG 
CCCCCTGGTC ACAAAGGGGA AAAGGGTGAC ATGGTCATAT CAAGAGTGAA AGGGCAAAAA 
GGAGAAAGAG GACTGGATGG GCCACCAGGA TTTCCGGGGC CACATGGACA AGATGGTGGG 
GATGGACGTC CTGGAGAAAG AGGGGATCCT GGGCCACGAG GGGACCATAA GGATGCAGCC 
CCGGGTGAGA GAGGGCTTCC CGGACTGCCA GGCCCTCCAG GAAGAACAGG ACCTGAGGGG 
CCTCCAGGCC TGGGATTTCC AGGCCCACCA GGACAGCGAG GCCTACCCGG AGAGCCCGGG 
CGTCCGGGCA CCAGAGGCTT TGATGGCACG AAGGGGCAGA AAGGTGACTC CATTCTGTGT 
AATGTAAGCT ACCCGGGAAA ACCAGGTCTG CCAGGTCTTG ATGGACCTCC AGGTCTGAAG 
GGGTTTCCAG GCCCCCCAGG GGCTCCTGGT ATGAGATGTC CAGATGGGCA GAAAGGCCAG 
CGGGGCAAAC CGGGAATGTC TGGGATTCCA GGTCCACCTG GTTTTCGTGG TGACATGGGA 
GATCCAGGCA TCAAAGGTGA AAAGGGGACC TCCCCTATTG GACCCCCAGG CCCACCTGGG 
TCTCCTGGAA AGGATGGCCA GAAAGGAATC CCAGGAGACC CTGCATTTGG TGACCCAGGA 
CCCCCTGGAG AGAGGGGTCT TCCAGGAGCA CCAGGCATGA AAGGACAGAA AGGTCACCCA 
GGGTGCCCAG GGGCTGGAGG CCCTCCTGGC ATCCCTGGAT CTCCAGGTCT CAAAGGTCCC 
AAAGGTAGAG AGGGAAGTCG TGGCTTTCCA GGGATCCCAG GATCCCCTGG CCATTCCTGT 
GAAAGAGGTG CTCCCGGGAT CCCAGGACAA CCGGGACTTC CTGGAACTCC AGGTGATCCA 
GGGGCCCCAG GTTGGAAAGG CCAGCCAGGA GACATGGGTC CCTCTGGACC AGCTGGGATG 
AAGGGCCTCC CTGGTCTCCC AGGCCTACCA GGAGCTGATG GTCTCCGAGG GCCTCCTGGG 
ATTCCAGGCC CCAATGGGGA AGATGGTCTA CCTGGTCTTC CAGGCCTGAA GGGACTCCCA 
GGGCTGCCTG GCTTCCCTGG TTTTCCAGGA GAGAGAGGAA AGCCAGGGCC AGATGGAGAA 
CCTGGAAGAA AGGGAGAGGT TGGAGAGAAA GGCTGGCCTG GCTTGAAGGG AGACCTGGGA 
GAGCGAGGCG CCAAAGGAGA CAGAGGACTT CCTGGTGATG CAGGAGAAGC AGTCACTTCC 
AGAAAGGGTG AACCTGGGGA TGCTGGGCCT CCAGGAGATG GTGGGTTCTC AGGAGAGAGA 
GGTGATAAAG GAAGCTCAGG GATGCGAGGG GGAAGAGGGG ATCCCGGAAG AGATGGACTA 
CCGGGACTCC ACAGAGGGCA GCCTGGGATA GATGGGCCTC CTGGGCCTCC TGGGCCCCCT 
GGCCCTCCAG GATCACCTGG ACTGAGAGGA GTCATTGGTT TCCCAGGATT TCCAGGTGAC 
CAGGGTGATC CAGGTTCTCC AGGACCCCCT GGATTCCCAG GAGATGATGG AGCAAGAGGA 
CCTAAAGGAT ACAAAGGTGA CCCTGCCAGT CAGTGTGGTC CACCTGGTCC AAAGGGTGAG 
CCAGGTAGTC CTGGATACCA AGGACGTACT GGAGTCCCCG GAGAGAAAGG CTTTCCTGGA 
GATGAAGGAC CCCGAGGACC ACCAGGCAGA CCTGGACAGC CTGGATCTTT TGGACCACCA 
GGGTGTCCAG GTGACCCAGG GATGCCCGGA CTCAAGGGAC ATCCAGGAGA AGTGGGAGAC 
CCTGGGCCAA GAGGTGATGC GGGAGATTTC GGGAGGCCAG GTCCTGCTGG AGTGAAAGGG 
CCTCTCGGGT CTCCTGGTCT GAATGGCTTA CATGGTCTGA AGGGTGAGAA AGGAACAAAA 
GGCGCCTCAG GTTTGCTCGA AATGGGTCCA CCTGGGCCAA TGGGGATGCC TGGGCAAAAA 
GGAGAGAAAG GAGACCCTGG GAGTCCAGGG ATTTCTCCTC CAGGTCTTCC TGGAGAAAAG 
GGCTTCCCAG GACCCCCAGG GAGACCAGGA CCACCTGGTC CCGCAGGTGC CCCAGGAAGA 
GCTGCTAAAG GTGACATTCC TGATCCAGGT CCACCTGGAG ACCGGGGACC TCCTGGCCCC 
GATGGCCCAA GAGGAGTACC TGGGCCTCCG GGTTCCCCTG GGAATGTTGA CCTTCTGAAA 
GGGGACCCAG GTGACTGTGG TTTGCCAGGA CCACCTGGCT CCCGAGGCCC ACCAGGCCCT 
CCAGGGTGTC AGGGTCCCCC AGGATGTGAT GGCAAAGATG GCCAGAAAGG ACCAATGGGA 
CTTCCAGGGT TGCCAGGGCC ACCTGGTCTT CCTGGGGCAC CTGGCGAGAA GGGATTACCT 
GGCCCTCCAG GCAGAAAAGG GCCAGTAGGT CCTCCAGGCT GCAGAGGTGA ACCTGGGCCT 
CCTGCAGACG TGGATTCCTG TCCTCGAATC CCAGGACTTC CTGGAGTGCC AGGTCCCAGA 
GGACCAGAAG GAGCTATGGG GGAACCTGGA CGGAGAGGCC TGCCTGGACC AGGGTGCAAA 
GGAGAGCCTG GACCGGATGG CAGACGGGGC CAGGATGGCA TCCCTGGATC TCCCGGGCCT 
CCTGGACGCA AAGGTGACAC TGGAGAGGCA GGGTGTCCTG GAGCACCAGG CCCTCCTGGT 
CCAACTGGTG ATCCTGGGCC CAAGGGGTTT GGCCCTGGAT CGCTCAGTGG CTTCCTCCTG 
GTTCTCCACA GTCAGACTGA CCAGGAACCG GCCTGCCCCG TGGGCATGCC TCGGCTTTGG 
ACAGGGTACA GCTTGTTGTA CATGGAAGGA CAGGAGAAGG CGCACAATCA AGATCTTGGT 
TTGGCAGGCT CTTGTCTTCC TGTGTTTAGC ACTCTGCCCT TTGCCTACTG CAACATCCAC 
CAAGTGTGCC ACTACGCCCA GAGAAATGAC AGGTCCTACT GGCTGTCCAG TGCGGCTCCT 
CTTCCTATGA TGCCACTCTC AGAGGAAGAG ATCCGCTCTT ACATCAGCCG CTGTGCGGTG 
TGTGAGGCCC CAGCACAGGC CGTGGCGGTC CACAGTCAGG ACCAATCCAT CCCACCGTGT 
CCACGGACAT GGAGGAGTCT CTGGATTGGG TACTCATTTC TGATGCACAC TGGAGCTGGG 
GACCAAGGAG GTGGGCAGGC ACTGATGTCA CCTGGCAGCT GCCTAGAGGA CTTCAGAGCT 
GCACCGTTCG TTGAATGCCA GGGCCGACAG GGAACCTGTC ACTTTTTTGC AAACGAGTAC 
AGCTTCTGGC TGACCACCGT GAACCCAGAC CTGCAGTTTG CCTCTGGCCC GTCACCAGAC 
ACCTTGAAAG AAGTTCAGGC CCAGCGCCGG AAAATCAGCA GGTGCCAGGT TTGCATGAAG 
CACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031761.1
CDS	352..5400
Translation	MRCFFRWTKSFVTAPWSLIFILFTIQYEYGSGKKYGGPCGGRNCSVCQCFPEKGSRGHPGPLGPQGPIGPLGPLGPIGIPGEKGERGDSGSPGPPGEKGDKGPTGVPGFPGVDGVPGHPGPPGPRGKPGVDGYNGSRGDPGYPGERGAPGPGGPPGQPGENGEKGRSVYITGGVKGIQGDRGDPGPPGLPGSRGAQGSPGPMGHAGAPGLAGPIGHPGSPGLKGNPATGLKGQRGEPGEVGQRGPPGPTLLVQPPDLSIYKGEKGVKGMPGMIGPPGPPGRKGAPGVGIKGEKGIPGFPGPRGEPGSHGPPGFPGFKGIQGAAGEPGLFGFLGPKGDLGDRGYPGPPGILLTPAPPLKGVPGDPGPPGYYGEIGDVGLPGPPGPPGRPGETCPGMMGPPGPPGVPGPPGFPGEAGVPGRLDCAPGKPGKPGLPGLPGAPGPEGPPGSDVIYCRPGCPGPMGEKGKVGPPGRRGAKGAKGNKGLCTCPPGPMGPPGPPGPPGRQGSKGDLGLPGWHGEKGDPGQPGAEGPPGPPGRPGAMGPPGHKGEKGDMVISRVKGQKGERGLDGPPGFPGPHGQDGGDGRPGERGDPGPRGDHKDAAPGERGLPGLPGPPGRTGPEGPPGLGFPGPPGQRGLPGEPGRPGTRGFDGTKGQKGDSILCNVSYPGKPGLPGLDGPPGLKGFPGPPGAPGMRCPDGQKGQRGKPGMSGIPGPPGFRGDMGDPGIKGEKGTSPIGPPGPPGSPGKDGQKGIPGDPAFGDPGPPGERGLPGAPGMKGQKGHPGCPGAGGPPGIPGSPGLKGPKGREGSRGFPGIPGSPGHSCERGAPGIPGQPGLPGTPGDPGAPGWKGQPGDMGPSGPAGMKGLPGLPGLPGADGLRGPPGIPGPNGEDGLPGLPGLKGLPGLPGFPGFPGERGKPGPDGEPGRKGEVGEKGWPGLKGDLGERGAKGDRGLPGDAGEAVTSRKGEPGDAGPPGDGGFSGERGDKGSSGMRGGRGDPGRDGLPGLHRGQPGIDGPPGPPGPPGPPGSPGLRGVIGFPGFPGDQGDPGSPGPPGFPGDDGARGPKGYKGDPASQCGPPGPKGEPGSPGYQGRTGVPGEKGFPGDEGPRGPPGRPGQPGSFGPPGCPGDPGMPGLKGHPGEVGDPGPRGDAGDFGRPGPAGVKGPLGSPGLNGLHGLKGEKGTKGASGLLEMGPPGPMGMPGQKGEKGDPGSPGISPPGLPGEKGFPGPPGRPGPPGPAGAPGRAAKGDIPDPGPPGDRGPPGPDGPRGVPGPPGSPGNVDLLKGDPGDCGLPGPPGSRGPPGPPGCQGPPGCDGKDGQKGPMGLPGLPGPPGLPGAPGEKGLPGPPGRKGPVGPPGCRGEPGPPADVDSCPRIPGLPGVPGPRGPEGAMGEPGRRGLPGPGCKGEPGPDGRRGQDGIPGSPGPPGRKGDTGEAGCPGAPGPPGPTGDPGPKGFGPGSLSGFLLVLHSQTDQEPACPVGMPRLWTGYSLLYMEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLSSAAPLPMMPLSEEEIRSYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFVECQGRQGTCHFFANEYSFWLTTVNPDLQFASGPSPDTLKEVQAQRRKISRCQVCMKHS

Target ORF information:

RefSeq Version	NM_007735.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 4 (Col4a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007735.2

ORF Insert Sequence:

ATGAGGTGCT TTTTCAGATG GACCAAGTCC TTTGTCACAG CCCCCTGGTC ACTAATATTT 
ATCCTCTTTA CTATACAATA TGAATATGGG AGTGGCAAGA AGTATGGCGG CCCCTGCGGA 
GGAAGAAACT GCTCTGTCTG CCAGTGTTTT CCTGAGAAGG GATCTCGGGG TCACCCAGGA 
CCACTGGGAC CACAGGGACC AATAGGGCCC TTGGGACCCC TGGGACCTAT TGGGATTCCA 
GGAGAAAAAG GAGAGAGAGG AGACAGTGGC TCACCTGGAC CACCAGGAGA GAAAGGAGAC 
AAGGGTCCAA CTGGTGTCCC TGGATTTCCA GGTGTGGATG GTGTACCTGG TCACCCAGGG 
CCTCCTGGAC CCAGAGGCAA ACCTGGTGTG GACGGCTACA ATGGCTCACG TGGTGATCCG 
GGCTATCCAG GAGAAAGAGG AGCTCCAGGC CCAGGCGGCC CCCCTGGTCA GCCTGGGGAA 
AATGGAGAAA AAGGAAGATC TGTGTACATT ACTGGTGGCG TTAAAGGTAT TCAGGGAGAC 
CGTGGGGACC CAGGACCACC CGGCTTACCA GGATCTAGGG GTGCACAAGG GTCACCAGGG 
CCCATGGGGC ATGCAGGCGC ACCAGGGCTA GCAGGACCTA TAGGTCATCC TGGAAGCCCT 
GGGTTGAAGG GAAATCCTGC CACTGGACTG AAGGGACAAA GGGGAGAGCC GGGTGAAGTT 
GGCCAGCGTG GTCCTCCTGG GCCCACGCTG TTGGTACAGC CGCCTGATTT GAGTATCTAT 
AAAGGAGAAA AGGGTGTAAA AGGAATGCCT GGAATGATTG GTCCCCCAGG ACCTCCAGGT 
CGCAAGGGAG CGCCTGGTGT CGGGATCAAA GGAGAGAAAG GTATCCCTGG GTTCCCAGGA 
CCCCGGGGTG AGCCTGGTTC GCATGGGCCT CCAGGTTTTC CAGGATTCAA GGGGATACAA 
GGAGCAGCTG GAGAGCCTGG GCTGTTCGGA TTTCTTGGTC CAAAGGGGGA TCTGGGAGAT 
CGCGGGTACC CGGGACCACC AGGCATTTTG TTGACTCCAG CTCCACCACT CAAAGGTGTT 
CCAGGGGACC CAGGACCCCC TGGCTACTAT GGGGAGATTG GAGATGTTGG ATTACCAGGT 
CCTCCAGGGC CCCCAGGCAG ACCAGGGGAA ACTTGTCCAG GCATGATGGG ACCTCCTGGA 
CCACCAGGGG TTCCTGGTCC TCCAGGATTT CCAGGAGAAG CTGGGGTTCC TGGAAGACTC 
GACTGTGCCC CAGGAAAACC TGGGAAGCCA GGGCTACCCG GCCTGCCTGG CGCACCAGGA 
CCAGAGGGGC CCCCTGGATC AGATGTGATA TACTGTCGCC CTGGGTGCCC TGGGCCAATG 
GGTGAAAAGG GAAAAGTGGG TCCTCCAGGA AGAAGAGGAG CCAAAGGAGC AAAAGGAAAC 
AAGGGACTCT GTACCTGTCC ACCTGGTCCC ATGGGACCCC CTGGTCCCCC AGGACCTCCT 
GGAAGACAAG GAAGTAAAGG AGACTTAGGG CTCCCTGGTT GGCATGGAGA AAAGGGCGAT 
CCAGGTCAGC CTGGTGCTGA AGGACCGCCA GGGCCACCAG GAAGACCTGG TGCCATGGGG 
CCCCCTGGTC ACAAAGGGGA AAAGGGTGAC ATGGTCATAT CAAGAGTGAA AGGGCAAAAA 
GGAGAAAGAG GACTGGATGG GCCACCAGGA TTTCCGGGGC CACATGGACA AGATGGTGGG 
GATGGACGTC CTGGAGAAAG AGGGGATCCT GGGCCACGAG GGGACCATAA GGATGCAGCC 
CCGGGTGAGA GAGGGCTTCC CGGACTGCCA GGCCCTCCAG GAAGAACAGG ACCTGAGGGG 
CCTCCAGGCC TGGGATTTCC AGGCCCACCA GGACAGCGAG GCCTACCCGG AGAGCCCGGG 
CGTCCGGGCA CCAGAGGCTT TGATGGCACG AAGGGGCAGA AAGGTGACTC CATTCTGTGT 
AATGTAAGCT ACCCGGGAAA ACCAGGTCTG CCAGGTCTTG ATGGACCTCC AGGTCTGAAG 
GGGTTTCCAG GCCCCCCAGG GGCTCCTGGT ATGAGATGTC CAGATGGGCA GAAAGGCCAG 
CGGGGCAAAC CGGGAATGTC TGGGATTCCA GGTCCACCTG GTTTTCGTGG TGACATGGGA 
GATCCAGGCA TCAAAGGTGA AAAGGGGACC TCCCCTATTG GACCCCCAGG CCCACCTGGG 
TCTCCTGGAA AGGATGGCCA GAAAGGAATC CCAGGAGACC CTGCATTTGG TGACCCAGGA 
CCCCCTGGAG AGAGGGGTCT TCCAGGAGCA CCAGGCATGA AAGGACAGAA AGGTCACCCA 
GGGTGCCCAG GGGCTGGAGG CCCTCCTGGC ATCCCTGGAT CTCCAGGTCT CAAAGGTCCC 
AAAGGTAGAG AGGGAAGTCG TGGCTTTCCA GGGATCCCAG GATCCCCTGG CCATTCCTGT 
GAAAGAGGTG CTCCCGGGAT CCCAGGACAA CCGGGACTTC CTGGAACTCC AGGTGATCCA 
GGGGCCCCAG GTTGGAAAGG CCAGCCAGGA GACATGGGTC CCTCTGGACC AGCTGGGATG 
AAGGGCCTCC CTGGTCTCCC AGGCCTACCA GGAGCTGATG GTCTCCGAGG GCCTCCTGGG 
ATTCCAGGCC CCAATGGGGA AGATGGTCTA CCTGGTCTTC CAGGCCTGAA GGGACTCCCA 
GGGCTGCCTG GCTTCCCTGG TTTTCCAGGA GAGAGAGGAA AGCCAGGGCC AGATGGAGAA 
CCTGGAAGAA AGGGAGAGGT TGGAGAGAAA GGCTGGCCTG GCTTGAAGGG AGACCTGGGA 
GAGCGAGGCG CCAAAGGAGA CAGAGGACTT CCTGGTGATG CAGGAGAAGC AGTCACTTCC 
AGAAAGGGTG AACCTGGGGA TGCTGGGCCT CCAGGAGATG GTGGGTTCTC AGGAGAGAGA 
GGTGATAAAG GAAGCTCAGG GATGCGAGGG GGAAGAGGGG ATCCCGGAAG AGATGGACTA 
CCGGGACTCC ACAGAGGGCA GCCTGGGATA GATGGGCCTC CTGGGCCTCC TGGGCCCCCT 
GGCCCTCCAG GATCACCTGG ACTGAGAGGA GTCATTGGTT TCCCAGGATT TCCAGGTGAC 
CAGGGTGATC CAGGTTCTCC AGGACCCCCT GGATTCCCAG GAGATGATGG AGCAAGAGGA 
CCTAAAGGAT ACAAAGGTGA CCCTGCCAGT CAGTGTGGTC CACCTGGTCC AAAGGGTGAG 
CCAGGTAGTC CTGGATACCA AGGACGTACT GGAGTCCCCG GAGAGAAAGG CTTTCCTGGA 
GATGAAGGAC CCCGAGGACC ACCAGGCAGA CCTGGACAGC CTGGATCTTT TGGACCACCA 
GGGTGTCCAG GTGACCCAGG GATGCCCGGA CTCAAGGGAC ATCCAGGAGA AGTGGGAGAC 
CCTGGGCCAA GAGGTGATGC GGGAGATTTC GGGAGGCCAG GTCCTGCTGG AGTGAAAGGG 
CCTCTCGGGT CTCCTGGTCT GAATGGCTTA CATGGTCTGA AGGGTGAGAA AGGAACAAAA 
GGCGCCTCAG GTTTGCTCGA AATGGGTCCA CCTGGGCCAA TGGGGATGCC TGGGCAAAAA 
GGAGAGAAAG GAGACCCTGG GAGTCCAGGG ATTTCTCCTC CAGGTCTTCC TGGAGAAAAG 
GGCTTCCCAG GACCCCCAGG GAGACCAGGA CCACCTGGTC CCGCAGGTGC CCCAGGAAGA 
GCTGCTAAAG GTGACATTCC TGATCCAGGT CCACCTGGAG ACCGGGGACC TCCTGGCCCC 
GATGGCCCAA GAGGAGTACC TGGGCCTCCG GGTTCCCCTG GGAATGTTGA CCTTCTGAAA 
GGGGACCCAG GTGACTGTGG TTTGCCAGGA CCACCTGGCT CCCGAGGCCC ACCAGGCCCT 
CCAGGGTGTC AGGGTCCCCC AGGATGTGAT GGCAAAGATG GCCAGAAAGG ACCAATGGGA 
CTTCCAGGGT TGCCAGGGCC ACCTGGTCTT CCTGGGGCAC CTGGCGAGAA GGGATTACCT 
GGCCCTCCAG GCAGAAAAGG GCCAGTAGGT CCTCCAGGCT GCAGAGGTGA ACCTGGGCCT 
CCTGCAGACG TGGATTCCTG TCCTCGAATC CCAGGACTTC CTGGAGTGCC AGGTCCCAGA 
GGACCAGAAG GAGCTATGGG GGAACCTGGA CGGAGAGGCC TGCCTGGACC AGGGTGCAAA 
GGAGAGCCTG GACCGGATGG CAGACGGGGC CAGGATGGCA TCCCTGGATC TCCCGGGCCT 
CCTGGACGCA AAGGTGACAC TGGAGAGGCA GGGTGTCCTG GAGCACCAGG CCCTCCTGGT 
CCAACTGGTG ATCCTGGGCC CAAGGGGTTT GGCCCTGGAT CGCTCAGTGG CTTCCTCCTG 
GTTCTCCACA GTCAGACTGA CCAGGAACCG GCCTGCCCCG TGGGCATGCC TCGGCTTTGG 
ACAGGGTACA GCTTGTTGTA CATGGAAGGA CAGGAGAAGG CGCACAATCA AGATCTTGGT 
TTGGCAGGCT CTTGTCTTCC TGTGTTTAGC ACTCTGCCCT TTGCCTACTG CAACATCCAC 
CAAGTGTGCC ACTACGCCCA GAGAAATGAC AGGTCCTACT GGCTGTCCAG TGCGGCTCCT 
CTTCCTATGA TGCCACTCTC AGAGGAAGAG ATCCGCTCTT ACATCAGCCG CTGTGCGGTG 
TGTGAGGCCC CAGCACAGGC CGTGGCGGTC CACAGTCAGG ACCAATCCAT CCCACCGTGT 
CCACGGACAT GGAGGAGTCT CTGGATTGGG TACTCATTTC TGATGCACAC TGGAGCTGGG 
GACCAAGGAG GTGGGCAGGC ACTGATGTCA CCTGGCAGCT GCCTAGAGGA CTTCAGAGCT 
GCACCGTTCG TTGAATGCCA GGGCCGACAG GGAACCTGTC ACTTTTTTGC AAACGAGTAC 
AGCTTCTGGC TGACCACCGT GAACCCAGAC CTGCAGTTTG CCTCTGGCCC GTCACCAGAC 
ACCTTGAAAG AAGTTCAGGC CCAGCGCCGG AAAATCAGCA GGTGCCAGGT TTGCATGAAG 
CACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a4 ( XM_011238652.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236954.1 Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X1, mRNA.

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CloneID	OMu67433
Clone ID Related Accession (Same CDS sequence)	XM_011238652.1
Accession Version	XM_011238652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATAG AGGGTCCAAC TGGTGTCCCT GGATTTCCAG GTGTGGATGG TGTACCTGGT 
CACCCAGGGC CTCCTGGACC CAGAGGCAAA CCTGGTGTGG ACGGCTACAA TGGCTCACGT 
GGTGATCCGG GCTATCCAGG AGAAAGAGGA GCTCCAGGCC CAGGCGGCCC CCCTGGTCAG 
CCTGGGGAAA ATGGAGAAAA AGGAAGATCT GTGTACATTA CTGGTGGCGT TAAAGGTATT 
CAGGGAGACC GTGGGGACCC AGGACCACCC GGCTTACCAG GATCTAGGGG TGCACAAGGG 
TCACCAGGGC CCATGGGGCA TGCAGGCGCA CCAGGGCTAG CAGGACCTAT AGGTCATCCT 
GGAAGCCCTG GGTTGAAGGG AAATCCTGCC ACTGGACTGA AGGGACAAAG GGGAGAGCCG 
GGTGAAGTTG GCCAGCGTGG TCCTCCTGGG CCCACGCTGT TGGTACAGCC GCCTGATTTG 
AGTATCTATA AAGGAGAAAA GGGTGTAAAA GGAATGCCTG GAATGATTGG TCCCCCAGGA 
CCTCCAGGTC GCAAGGGAGC GCCTGGTGTC GGGATCAAAG GAGAGAAAGG TATCCCTGGG 
TTCCCAGGAC CCCGGGGTGA GCCTGGTTCG CATGGGCCTC CAGGTTTTCC AGGATTCAAG 
GGGATACAAG GAGCAGCTGG AGAGCCTGGG CTGTTCGGAT TTCTTGGTCC AAAGGGGGAT 
CTGGGAGATC GCGGGTACCC GGGACCACCA GGCATTTTGT TGACTCCAGC TCCACCACTC 
AAAGGTGTTC CAGGGGACCC AGGACCCCCT GGCTACTATG GGGAGATTGG AGATGTTGGA 
TTACCAGGTC CTCCAGGGCC CCCAGGCAGA CCAGGGGAAA CTTGTCCAGG CATGATGGGA 
CCTCCTGGAC CACCAGGGGT TCCTGGTCCT CCAGGATTTC CAGGAGAAGC TGGGGTTCCT 
GGAAGACTCG ACTGTGCCCC AGGAAAACCT GGGAAGCCAG GGCTACCCGG CCTGCCTGGC 
GCACCAGGAC CAGAGGGGCC CCCTGGATCA GATGTGATAT ACTGTCGCCC TGGGTGCCCT 
GGGCCAATGG GTGAAAAGGG AAAAGTGGGT CCTCCAGGAA GAAGAGGAGC CAAAGGAGCA 
AAAGGAAACA AGGGACTCTG TACCTGTCCA CCTGGTCCCA TGGGACCCCC TGGTCCCCCA 
GGACCTCCTG GAAGACAAGG AAGTAAAGGA GACTTAGGGC TCCCTGGTTG GCATGGAGAA 
AAGGGCGATC CAGGTCAGCC TGGTGCTGAA GGACCGCCAG GGCCACCAGG AAGACCTGGT 
GCCATGGGGC CCCCTGGTCA CAAAGGGGAA AAGGGTGACA TGGTCATATC AAGAGTGAAA 
GGGCAAAAAG GAGAAAGAGG ACTGGATGGG CCACCAGGAT TTCCGGGGCC ACATGGACAA 
GATGGTGGGG ATGGACGTCC TGGAGAAAGA GGGGATCCTG GGCCACGAGG GGACCATAAG 
GATGCAGCCC CGGGTGAGAG AGGGCTTCCC GGACTGCCAG GCCCTCCAGG AAGAACAGGA 
CCTGAGGGGC CTCCAGGCCT GGGATTTCCA GGCCCACCAG GACAGCGAGG CCTACCCGGA 
GAGCCCGGGC GTCCGGGCAC CAGAGGCTTT GATGGCACGA AGGGGCAGAA AGGTGACTCC 
ATTCTGTGTA ATGTAAGCTA CCCGGGAAAA CCAGGTCTGC CAGGTCTTGA TGGACCTCCA 
GGTCTGAAGG GGTTTCCAGG CCCCCCAGGG GCTCCTGGTA TGAGATGTCC AGATGGGCAG 
AAAGGCCAGC GGGGCAAACC GGGAATGTCT GGGATTCCAG GTCCACCTGG TTTTCGTGGT 
GACATGGGAG ATCCAGGCAT CAAAGGTGAA AAGGGGACCT CCCCTATTGG ACCCCCAGGC 
CCACCTGGGT CTCCTGGAAA GGATGGCCAG AAAGGAATCC CAGGAGACCC TGCATTTGGT 
GACCCAGGAC CCCCTGGAGA GAGGGGTCTT CCAGGAGCAC CAGGCATGAA AGGACAGAAA 
GGTCACCCAG GGTGCCCAGG GGCTGGAGGC CCTCCTGGCA TCCCTGGATC TCCAGGTCTC 
AAAGGTCCCA AAGGTAGAGA GGGAAGTCGT GGCTTTCCAG GGATCCCAGG ATCCCCTGGC 
CATTCCTGTG AAAGAGGTGC TCCCGGGATC CCAGGACAAC CGGGACTTCC TGGAACTCCA 
GGTGATCCAG GGGCCCCAGG TTGGAAAGGC CAGCCAGGAG ACATGGGTCC CTCTGGACCA 
GCTGGGATGA AGGGCCTCCC TGGTCTCCCA GGCCTACCAG GAGCTGATGG TCTCCGAGGG 
CCTCCTGGGA TTCCAGGCCC CAATGGGGAA GATGGTCTAC CTGGTCTTCC AGGCCTGAAG 
GGACTCCCAG GGCTGCCTGG CTTCCCTGGT TTTCCAGGAG AGAGAGGAAA GCCAGGGCCA 
GATGGAGAAC CTGGAAGAAA GGGAGAGGTT GGAGAGAAAG GCTGGCCTGG CTTGAAGGGA 
GACCTGGGAG AGCGAGGCGC CAAAGGAGAC AGAGGACTTC CTGGTGATGC AGGAGAAGCA 
GTCACTTCCA GAAAGGGTGA ACCTGGGGAT GCTGGGCCTC CAGGAGATGG TGGGTTCTCA 
GGAGAGAGAG GTGATAAAGG AAGCTCAGGG ATGCGAGGGG GAAGAGGGGA TCCCGGAAGA 
GATGGACTAC CGGGACTCCA CAGAGGGCAG CCTGGGATAG ATGGGCCTCC TGGGCCTCCT 
GGGCCCCCTG GCCCTCCAGG ATCACCTGGA CTGAGAGGAG TCATTGGTTT CCCAGGATTT 
CCAGGTGACC AGGGTGATCC AGGTTCTCCA GGACCCCCTG GATTCCCAGG AGATGATGGA 
GCAAGAGGAC CTAAAGGATA CAAAGGTGAC CCTGCCAGTC AGTGTGGTCC ACCTGGTCCA 
AAGGGTGAGC CAGGTAGTCC TGGATACCAA GGACGTACTG GAGTCCCCGG AGAGAAAGGC 
TTTCCTGGAG ATGAAGGACC CCGAGGACCA CCAGGCAGAC CTGGACAGCC TGGATCTTTT 
GGACCACCAG GGTGTCCAGG TGACCCAGGG ATGCCCGGAC TCAAGGGACA TCCAGGAGAA 
GTGGGAGACC CTGGGCCAAG AGGTGATGCG GGAGATTTCG GGAGGCCAGG TCCTGCTGGA 
GTGAAAGGGC CTCTCGGGTC TCCTGGTCTG AATGGCTTAC ATGGTCTGAA GGGTGAGAAA 
GGAACAAAAG GCGCCTCAGG TTTGCTCGAA ATGGGTCCAC CTGGGCCAAT GGGGATGCCT 
GGGCAAAAAG GAGAGAAAGG AGACCCTGGG AGTCCAGGGA TTTCTCCTCC AGGTCTTCCT 
GGAGAAAAGG GCTTCCCAGG ACCCCCAGGG AGACCAGGAC CACCTGGTCC CGCAGGTGCC 
CCAGGAAGAG CTGCTAAAGG TGACATTCCT GATCCAGGTC CACCTGGAGA CCGGGGACCT 
CCTGGCCCCG ATGGCCCAAG AGGAGTACCT GGGCCTCCGG GTTCCCCTGG GAATGTTGAC 
CTTCTGAAAG GGGACCCAGG TGACTGTGGT TTGCCAGGAC CACCTGGCTC CCGAGGCCCA 
CCAGGCCCTC CAGGGTGTCA GGGTCCCCCA GGATGTGATG GCAAAGATGG CCAGAAAGGA 
CCAATGGGAC TTCCAGGGTT GCCAGGGCCA CCTGGTCTTC CTGGGGCACC TGGCGAGAAG 
GGATTACCTG GCCCTCCAGG CAGAAAAGGG CCAGTAGGTC CTCCAGGCTG CAGAGGTGAA 
CCTGGGCCTC CTGCAGACGT GGATTCCTGT CCTCGAATCC CAGGACTTCC TGGAGTGCCA 
GGTCCCAGAG GACCAGAAGG AGCTATGGGG GAACCTGGAC GGAGAGGCCT GCCTGGACCA 
GGGTGCAAAG GAGAGCCTGG ACCGGATGGC AGACGGGGCC AGGATGGCAT CCCTGGATCT 
CCCGGGCCTC CTGGACGCAA AGGTGACACT GGAGAGGCAG GGTGTCCTGG AGCACCAGGC 
CCTCCTGGTC CAACTGGTGA TCCTGGGCCC AAGGGGTTTG GCCCTGGATC GCTCAGTGGC 
TTCCTCCTGG TTCTCCACAG TCAGACTGAC CAGGAACCGG CCTGCCCCGT GGGCATGCCT 
CGGCTTTGGA CAGGGTACAG CTTGTTGTAC ATGGAAGGAC AGGAGAAGGC GCACAATCAA 
GATCTTGGTT TGGCAGGCTC TTGTCTTCCT GTGTTTAGCA CTCTGCCCTT TGCCTACTGC 
AACATCCACC AAGTGTGCCA CTACGCCCAG AGAAATGACA GGTCCTACTG GCTGTCCAGT 
GCGGCTCCTC TTCCTATGAT GCCACTCTCA GAGGAAGAGA TCCGCTCTTA CATCAGCCGC 
TGTGCGGTGT GTGAGGCCCC AGCACAGGCC GTGGCGGTCC ACAGTCAGGA CCAATCCATC 
CCACCGTGTC CACGGACATG GAGGAGTCTC TGGATTGGGT ACTCATTTCT GATGCACACT 
GGAGCTGGGG ACCAAGGAGG TGGGCAGGCA CTGATGTCAC CTGGCAGCTG CCTAGAGGAC 
TTCAGAGCTG CACCGTTCGT TGAATGCCAG GGCCGACAGG GAACCTGTCA CTTTTTTGCA 
AACGAGTACA GCTTCTGGCT GACCACCGTG AACCCAGACC TGCAGTTTGC CTCTGGCCCG 
TCACCAGACA CCTTGAAAGA AGTTCAGGCC CAGCGCCGGA AAATCAGCAG GTGCCAGGTT 
TGCATGAAGC ACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236954.1
CDS	16..4773
Translation	MDIEGPTGVPGFPGVDGVPGHPGPPGPRGKPGVDGYNGSRGDPGYPGERGAPGPGGPPGQPGENGEKGRSVYITGGVKGIQGDRGDPGPPGLPGSRGAQGSPGPMGHAGAPGLAGPIGHPGSPGLKGNPATGLKGQRGEPGEVGQRGPPGPTLLVQPPDLSIYKGEKGVKGMPGMIGPPGPPGRKGAPGVGIKGEKGIPGFPGPRGEPGSHGPPGFPGFKGIQGAAGEPGLFGFLGPKGDLGDRGYPGPPGILLTPAPPLKGVPGDPGPPGYYGEIGDVGLPGPPGPPGRPGETCPGMMGPPGPPGVPGPPGFPGEAGVPGRLDCAPGKPGKPGLPGLPGAPGPEGPPGSDVIYCRPGCPGPMGEKGKVGPPGRRGAKGAKGNKGLCTCPPGPMGPPGPPGPPGRQGSKGDLGLPGWHGEKGDPGQPGAEGPPGPPGRPGAMGPPGHKGEKGDMVISRVKGQKGERGLDGPPGFPGPHGQDGGDGRPGERGDPGPRGDHKDAAPGERGLPGLPGPPGRTGPEGPPGLGFPGPPGQRGLPGEPGRPGTRGFDGTKGQKGDSILCNVSYPGKPGLPGLDGPPGLKGFPGPPGAPGMRCPDGQKGQRGKPGMSGIPGPPGFRGDMGDPGIKGEKGTSPIGPPGPPGSPGKDGQKGIPGDPAFGDPGPPGERGLPGAPGMKGQKGHPGCPGAGGPPGIPGSPGLKGPKGREGSRGFPGIPGSPGHSCERGAPGIPGQPGLPGTPGDPGAPGWKGQPGDMGPSGPAGMKGLPGLPGLPGADGLRGPPGIPGPNGEDGLPGLPGLKGLPGLPGFPGFPGERGKPGPDGEPGRKGEVGEKGWPGLKGDLGERGAKGDRGLPGDAGEAVTSRKGEPGDAGPPGDGGFSGERGDKGSSGMRGGRGDPGRDGLPGLHRGQPGIDGPPGPPGPPGPPGSPGLRGVIGFPGFPGDQGDPGSPGPPGFPGDDGARGPKGYKGDPASQCGPPGPKGEPGSPGYQGRTGVPGEKGFPGDEGPRGPPGRPGQPGSFGPPGCPGDPGMPGLKGHPGEVGDPGPRGDAGDFGRPGPAGVKGPLGSPGLNGLHGLKGEKGTKGASGLLEMGPPGPMGMPGQKGEKGDPGSPGISPPGLPGEKGFPGPPGRPGPPGPAGAPGRAAKGDIPDPGPPGDRGPPGPDGPRGVPGPPGSPGNVDLLKGDPGDCGLPGPPGSRGPPGPPGCQGPPGCDGKDGQKGPMGLPGLPGPPGLPGAPGEKGLPGPPGRKGPVGPPGCRGEPGPPADVDSCPRIPGLPGVPGPRGPEGAMGEPGRRGLPGPGCKGEPGPDGRRGQDGIPGSPGPPGRKGDTGEAGCPGAPGPPGPTGDPGPKGFGPGSLSGFLLVLHSQTDQEPACPVGMPRLWTGYSLLYMEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLSSAAPLPMMPLSEEEIRSYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFVECQGRQGTCHFFANEYSFWLTTVNPDLQFASGPSPDTLKEVQAQRRKISRCQVCMKHS

Target ORF information:

RefSeq Version	XM_011238652.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238652.1

ORF Insert Sequence:

ATGGACATAG AGGGTCCAAC TGGTGTCCCT GGATTTCCAG GTGTGGATGG TGTACCTGGT 
CACCCAGGGC CTCCTGGACC CAGAGGCAAA CCTGGTGTGG ACGGCTACAA TGGCTCACGT 
GGTGATCCGG GCTATCCAGG AGAAAGAGGA GCTCCAGGCC CAGGCGGCCC CCCTGGTCAG 
CCTGGGGAAA ATGGAGAAAA AGGAAGATCT GTGTACATTA CTGGTGGCGT TAAAGGTATT 
CAGGGAGACC GTGGGGACCC AGGACCACCC GGCTTACCAG GATCTAGGGG TGCACAAGGG 
TCACCAGGGC CCATGGGGCA TGCAGGCGCA CCAGGGCTAG CAGGACCTAT AGGTCATCCT 
GGAAGCCCTG GGTTGAAGGG AAATCCTGCC ACTGGACTGA AGGGACAAAG GGGAGAGCCG 
GGTGAAGTTG GCCAGCGTGG TCCTCCTGGG CCCACGCTGT TGGTACAGCC GCCTGATTTG 
AGTATCTATA AAGGAGAAAA GGGTGTAAAA GGAATGCCTG GAATGATTGG TCCCCCAGGA 
CCTCCAGGTC GCAAGGGAGC GCCTGGTGTC GGGATCAAAG GAGAGAAAGG TATCCCTGGG 
TTCCCAGGAC CCCGGGGTGA GCCTGGTTCG CATGGGCCTC CAGGTTTTCC AGGATTCAAG 
GGGATACAAG GAGCAGCTGG AGAGCCTGGG CTGTTCGGAT TTCTTGGTCC AAAGGGGGAT 
CTGGGAGATC GCGGGTACCC GGGACCACCA GGCATTTTGT TGACTCCAGC TCCACCACTC 
AAAGGTGTTC CAGGGGACCC AGGACCCCCT GGCTACTATG GGGAGATTGG AGATGTTGGA 
TTACCAGGTC CTCCAGGGCC CCCAGGCAGA CCAGGGGAAA CTTGTCCAGG CATGATGGGA 
CCTCCTGGAC CACCAGGGGT TCCTGGTCCT CCAGGATTTC CAGGAGAAGC TGGGGTTCCT 
GGAAGACTCG ACTGTGCCCC AGGAAAACCT GGGAAGCCAG GGCTACCCGG CCTGCCTGGC 
GCACCAGGAC CAGAGGGGCC CCCTGGATCA GATGTGATAT ACTGTCGCCC TGGGTGCCCT 
GGGCCAATGG GTGAAAAGGG AAAAGTGGGT CCTCCAGGAA GAAGAGGAGC CAAAGGAGCA 
AAAGGAAACA AGGGACTCTG TACCTGTCCA CCTGGTCCCA TGGGACCCCC TGGTCCCCCA 
GGACCTCCTG GAAGACAAGG AAGTAAAGGA GACTTAGGGC TCCCTGGTTG GCATGGAGAA 
AAGGGCGATC CAGGTCAGCC TGGTGCTGAA GGACCGCCAG GGCCACCAGG AAGACCTGGT 
GCCATGGGGC CCCCTGGTCA CAAAGGGGAA AAGGGTGACA TGGTCATATC AAGAGTGAAA 
GGGCAAAAAG GAGAAAGAGG ACTGGATGGG CCACCAGGAT TTCCGGGGCC ACATGGACAA 
GATGGTGGGG ATGGACGTCC TGGAGAAAGA GGGGATCCTG GGCCACGAGG GGACCATAAG 
GATGCAGCCC CGGGTGAGAG AGGGCTTCCC GGACTGCCAG GCCCTCCAGG AAGAACAGGA 
CCTGAGGGGC CTCCAGGCCT GGGATTTCCA GGCCCACCAG GACAGCGAGG CCTACCCGGA 
GAGCCCGGGC GTCCGGGCAC CAGAGGCTTT GATGGCACGA AGGGGCAGAA AGGTGACTCC 
ATTCTGTGTA ATGTAAGCTA CCCGGGAAAA CCAGGTCTGC CAGGTCTTGA TGGACCTCCA 
GGTCTGAAGG GGTTTCCAGG CCCCCCAGGG GCTCCTGGTA TGAGATGTCC AGATGGGCAG 
AAAGGCCAGC GGGGCAAACC GGGAATGTCT GGGATTCCAG GTCCACCTGG TTTTCGTGGT 
GACATGGGAG ATCCAGGCAT CAAAGGTGAA AAGGGGACCT CCCCTATTGG ACCCCCAGGC 
CCACCTGGGT CTCCTGGAAA GGATGGCCAG AAAGGAATCC CAGGAGACCC TGCATTTGGT 
GACCCAGGAC CCCCTGGAGA GAGGGGTCTT CCAGGAGCAC CAGGCATGAA AGGACAGAAA 
GGTCACCCAG GGTGCCCAGG GGCTGGAGGC CCTCCTGGCA TCCCTGGATC TCCAGGTCTC 
AAAGGTCCCA AAGGTAGAGA GGGAAGTCGT GGCTTTCCAG GGATCCCAGG ATCCCCTGGC 
CATTCCTGTG AAAGAGGTGC TCCCGGGATC CCAGGACAAC CGGGACTTCC TGGAACTCCA 
GGTGATCCAG GGGCCCCAGG TTGGAAAGGC CAGCCAGGAG ACATGGGTCC CTCTGGACCA 
GCTGGGATGA AGGGCCTCCC TGGTCTCCCA GGCCTACCAG GAGCTGATGG TCTCCGAGGG 
CCTCCTGGGA TTCCAGGCCC CAATGGGGAA GATGGTCTAC CTGGTCTTCC AGGCCTGAAG 
GGACTCCCAG GGCTGCCTGG CTTCCCTGGT TTTCCAGGAG AGAGAGGAAA GCCAGGGCCA 
GATGGAGAAC CTGGAAGAAA GGGAGAGGTT GGAGAGAAAG GCTGGCCTGG CTTGAAGGGA 
GACCTGGGAG AGCGAGGCGC CAAAGGAGAC AGAGGACTTC CTGGTGATGC AGGAGAAGCA 
GTCACTTCCA GAAAGGGTGA ACCTGGGGAT GCTGGGCCTC CAGGAGATGG TGGGTTCTCA 
GGAGAGAGAG GTGATAAAGG AAGCTCAGGG ATGCGAGGGG GAAGAGGGGA TCCCGGAAGA 
GATGGACTAC CGGGACTCCA CAGAGGGCAG CCTGGGATAG ATGGGCCTCC TGGGCCTCCT 
GGGCCCCCTG GCCCTCCAGG ATCACCTGGA CTGAGAGGAG TCATTGGTTT CCCAGGATTT 
CCAGGTGACC AGGGTGATCC AGGTTCTCCA GGACCCCCTG GATTCCCAGG AGATGATGGA 
GCAAGAGGAC CTAAAGGATA CAAAGGTGAC CCTGCCAGTC AGTGTGGTCC ACCTGGTCCA 
AAGGGTGAGC CAGGTAGTCC TGGATACCAA GGACGTACTG GAGTCCCCGG AGAGAAAGGC 
TTTCCTGGAG ATGAAGGACC CCGAGGACCA CCAGGCAGAC CTGGACAGCC TGGATCTTTT 
GGACCACCAG GGTGTCCAGG TGACCCAGGG ATGCCCGGAC TCAAGGGACA TCCAGGAGAA 
GTGGGAGACC CTGGGCCAAG AGGTGATGCG GGAGATTTCG GGAGGCCAGG TCCTGCTGGA 
GTGAAAGGGC CTCTCGGGTC TCCTGGTCTG AATGGCTTAC ATGGTCTGAA GGGTGAGAAA 
GGAACAAAAG GCGCCTCAGG TTTGCTCGAA ATGGGTCCAC CTGGGCCAAT GGGGATGCCT 
GGGCAAAAAG GAGAGAAAGG AGACCCTGGG AGTCCAGGGA TTTCTCCTCC AGGTCTTCCT 
GGAGAAAAGG GCTTCCCAGG ACCCCCAGGG AGACCAGGAC CACCTGGTCC CGCAGGTGCC 
CCAGGAAGAG CTGCTAAAGG TGACATTCCT GATCCAGGTC CACCTGGAGA CCGGGGACCT 
CCTGGCCCCG ATGGCCCAAG AGGAGTACCT GGGCCTCCGG GTTCCCCTGG GAATGTTGAC 
CTTCTGAAAG GGGACCCAGG TGACTGTGGT TTGCCAGGAC CACCTGGCTC CCGAGGCCCA 
CCAGGCCCTC CAGGGTGTCA GGGTCCCCCA GGATGTGATG GCAAAGATGG CCAGAAAGGA 
CCAATGGGAC TTCCAGGGTT GCCAGGGCCA CCTGGTCTTC CTGGGGCACC TGGCGAGAAG 
GGATTACCTG GCCCTCCAGG CAGAAAAGGG CCAGTAGGTC CTCCAGGCTG CAGAGGTGAA 
CCTGGGCCTC CTGCAGACGT GGATTCCTGT CCTCGAATCC CAGGACTTCC TGGAGTGCCA 
GGTCCCAGAG GACCAGAAGG AGCTATGGGG GAACCTGGAC GGAGAGGCCT GCCTGGACCA 
GGGTGCAAAG GAGAGCCTGG ACCGGATGGC AGACGGGGCC AGGATGGCAT CCCTGGATCT 
CCCGGGCCTC CTGGACGCAA AGGTGACACT GGAGAGGCAG GGTGTCCTGG AGCACCAGGC 
CCTCCTGGTC CAACTGGTGA TCCTGGGCCC AAGGGGTTTG GCCCTGGATC GCTCAGTGGC 
TTCCTCCTGG TTCTCCACAG TCAGACTGAC CAGGAACCGG CCTGCCCCGT GGGCATGCCT 
CGGCTTTGGA CAGGGTACAG CTTGTTGTAC ATGGAAGGAC AGGAGAAGGC GCACAATCAA 
GATCTTGGTT TGGCAGGCTC TTGTCTTCCT GTGTTTAGCA CTCTGCCCTT TGCCTACTGC 
AACATCCACC AAGTGTGCCA CTACGCCCAG AGAAATGACA GGTCCTACTG GCTGTCCAGT 
GCGGCTCCTC TTCCTATGAT GCCACTCTCA GAGGAAGAGA TCCGCTCTTA CATCAGCCGC 
TGTGCGGTGT GTGAGGCCCC AGCACAGGCC GTGGCGGTCC ACAGTCAGGA CCAATCCATC 
CCACCGTGTC CACGGACATG GAGGAGTCTC TGGATTGGGT ACTCATTTCT GATGCACACT 
GGAGCTGGGG ACCAAGGAGG TGGGCAGGCA CTGATGTCAC CTGGCAGCTG CCTAGAGGAC 
TTCAGAGCTG CACCGTTCGT TGAATGCCAG GGCCGACAGG GAACCTGTCA CTTTTTTGCA 
AACGAGTACA GCTTCTGGCT GACCACCGTG AACCCAGACC TGCAGTTTGC CTCTGGCCCG 
TCACCAGACA CCTTGAAAGA AGTTCAGGCC CAGCGCCGGA AAATCAGCAG GTGCCAGGTT 
TGCATGAAGC ACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a4 ( XM_011238653.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236955.1 Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X2, mRNA.

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CloneID	OMu67434
Clone ID Related Accession (Same CDS sequence)	XM_011238653.3 , XM_011238653.2
Accession Version	XM_011238653.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-4(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTGCT TTTTCAGATG GACCAAGTCC TTTGTCACAG CCCCCTGGTC ACTAATATTT 
ATCCTCTTTA CTATACAATA TGAATATGGG AGTGGCAAGA AGTATGGCGG CCCCTGCGGA 
GGAAGAAACT GCTCTGTCTG CCAGTGTTTT CCTGAGAAGG GATCTCGGGG TCACCCAGGA 
CCACTGGGAC CACAGGGACC AATAGGGCCC TTGGGACCCC TGGGACCTAT TGGGATTCCA 
GGAGAAAAAG GAGAGAGAGG AGACAGTGGC TCACCTGGAC CACCAGGAGA GAAAGGAGAC 
AAGGGTCCAA CTGGTGTCCC TGGATTTCCA GGTGTGGATG GTGTACCTGG TCACCCAGGG 
CCTCCTGGAC CCAGAGGCAA ACCTGGTGTG GACGGCTACA ATGGCTCACG TGGTGATCCG 
GGCTATCCAG GAGAAAGAGG AGCTCCAGGC CCAGGCGGCC CCCCTGGTCA GCCTGGGGAA 
AATGGAGAAA AAGGAAGATC TGTGTACATT ACTGGTGGCG TTAAAGGTAT TCAGGGAGAC 
CGTGGGGACC CAGGACCACC CGGCTTACCA GGATCTAGGG GTGCACAAGG GTCACCAGGG 
CCCATGGGGC ATGCAGGCGC ACCAGGGCTA GCAGGACCTA TAGGTCATCC TGGAAGCCCT 
GGGTTGAAGG GAAATCCTGC CACTGGACTG AAGGGACAAA GGGGAGAGCC GGGTGAAGTT 
GGCCAGCGTG GTCCTCCTGG GCCCACGCTG TTGGTACAGC CGCCTGATTT GAGTATCTAT 
AAAGGAGAAA AGGGTGTAAA AGGAATGCCT GGAATGATTG GTCCCCCAGG ACCTCCAGGT 
CGCAAGGGAG CGCCTGGTGT CGGGATCAAA GGAGAGAAAG GTATCCCTGG GTTCCCAGGA 
CCCCGGGGTG AGCCTGGTTC GCATGGGCCT CCAGGTTTTC CAGGATTCAA GGGGATACAA 
GGAGCAGCTG GAGAGCCTGG GCTGTTCGGA TTTCTTGGTC CAAAGGGGGA TCTGGGAGAT 
CGCGGGTACC CGGGACCACC AGGCATTTTG TTGACTCCAG CTCCACCACT CAAAGGTGTT 
CCAGGGGACC CAGGACCCCC TGGCTACTAT GGGGAGATTG GAGATGTTGG ATTACCAGGT 
CCTCCAGGGC CCCCAGGCAG ACCAGGGGAA ACTTGTCCAG GCATGATGGG ACCTCCTGGA 
CCACCAGGGG TTCCTGGTCC TCCAGGATTT CCAGGAGAAG CTGGGGTTCC TGGAAGACTC 
GACTGTGCCC CAGGAAAACC TGGGAAGCCA GGGCTACCCG GCCTGCCTGG CGCACCAGGA 
CCAGAGGGGC CCCCTGGATC AGATGTGATA TACTGTCGCC CTGGGTGCCC TGGGCCAATG 
GGTGAAAAGG GAAAAGTGGG TCCTCCAGGA AGAAGAGGAG CCAAAGGAGC AAAAGGAAAC 
AAGGGACTCT GTACCTGTCC ACCTGGTCCC ATGGGACCCC CTGGTCCCCC AGGACCTCCT 
GGAAGACAAG GAAGTAAAGG AGACTTAGGG CTCCCTGGTT GGCATGGAGA AAAGGGCGAT 
CCAGGTCAGC CTGGTGCTGA AGGACCGCCA GGGCCACCAG GAAGACCTGG TGCCATGGGG 
CCCCCTGGTC ACAAAGGGGA AAAGGGTGAC ATGGTCATAT CAAGAGTGAA AGGGCAAAAA 
GGAGAAAGAG GACTGGATGG GCCACCAGGA TTTCCGGGGC CACATGGACA AGATGGTGGG 
GATGGACGTC CTGGAGAAAG AGGGGATCCT GGGCCACGAG GGGACCATAA GGATGCAGCC 
CCGGGTGAGA GAGGGCTTCC CGGACTGCCA GGCCCTCCAG GAAGAACAGG ACCTGAGGGG 
CCTCCAGGCC TGGGATTTCC AGGCCCACCA GGACAGCGAG GCCTACCCGG AGAGCCCGGG 
CGTCCGGGCA CCAGAGGCTT TGATGGCACG AAGGGGCAGA AAGGTGACTC CATTCTGTGT 
AATGTAAGCT ACCCGGGAAA ACCAGGTCTG CCAGGTCTTG ATGGACCTCC AGGTCTGAAG 
GGGTTTCCAG GCCCCCCAGG GGCTCCTGGT ATGAGATGTC CAGATGGGCA GAAAGGCCAG 
CGGGGCAAAC CGGGAATGTC TGGGATTCCA GGTCCACCTG GTTTTCGTGG TGACATGGGA 
GATCCAGGCA TCAAAGGTGA AAAGGGGACC TCCCCTATTG GACCCCCAGG CCCACCTGGG 
TCTCCTGGAA AGGATGGCCA GAAAGGAATC CCAGGAGACC CTGCATTTGG TGACCCAGGA 
CCCCCTGGAG AGAGGGGTCT TCCAGGAGCA CCAGGCATGA AAGGACAGAA AGGTCACCCA 
GGGTGCCCAG GGGCTGGAGG CCCTCCTGGC ATCCCTGGAT CTCCAGGTCT CAAAGGTCCC 
AAAGGTAGAG AGGGAAGTCG TGGCTTTCCA GGGATCCCAG GATCCCCTGG CCATTCCTGT 
GAAAGAGGTG CTCCCGGGAT CCCAGGACAA CCGGGACTTC CTGGAACTCC AGGTGATCCA 
GGGGCCCCAG GTTGGAAAGG CCAGCCAGGA GACATGGGTC CCTCTGGACC AGCTGGGATG 
AAGGGCCTCC CTGGTCTCCC AGGCCTACCA GGAGCTGATG GTCTCCGAGG GCCTCCTGGG 
ATTCCAGGCC CCAATGGGGA AGATGGTCTA CCTGGTCTTC CAGGCCTGAA GGGACTCCCA 
GGGCTGCCTG GCTTCCCTGG TTTTCCAGGA GAGAGAGGAA AGCCAGGGCC AGATGGAGAA 
CCTGGAAGAA AGGGAGAGGT TGGAGAGAAA GGCTGGCCTG GCTTGAAGGG AGACCTGGGA 
GAGCGAGGCG CCAAAGGAGA CAGAGGACTT CCTGGTGATG CAGGAGAAGC AGTCACTTCC 
AGAAAGGGTG AACCTGGGGA TGCTGGGCCT CCAGGAGATG GTGGGTTCTC AGGAGAGAGA 
GGTGATAAAG GAAGCTCAGG GATGCGAGGG GGAAGAGGGG ATCCCGGAAG AGATGGACTA 
CCGGGACTCC ACAGAGGGCA GCCTGGGATA GATGGGCCTC CTGGGCCTCC TGGGCCCCCT 
GGCCCTCCAG GATCACCTGG ACTGAGAGGA GTCATTGGTT TCCCAGGATT TCCAGGTGAC 
CAGGGTGATC CAGGTTCTCC AGGACCCCCT GGATTCCCAG GAGATGATGG AGCAAGAGGA 
CCTAAAGGAT ACAAAGGTGA CCCTGCCAGT CAGTGTGGTC CACCTGGTCC AAAGGGTGAG 
CCAGGTAGTC CTGGATACCA AGGACGTACT GGAGTCCCCG GAGAGAAAGG CTTTCCTGGA 
GATGAAGGAC CCCGAGGACC ACCAGGCAGA CCTGGACAGC CTGGATCTTT TGGACCACCA 
GGGTGTCCAG GTGACCCAGG GATGCCCGGA CTCAAGGGAC ATCCAGGAGA AGTGGGAGAC 
CCTGGGCCAA GAGGTGATGC GGGAGATTTC GGGAGGCCAG GTCCTGCTGG AGTGAAAGGG 
CCTCTCGGGT CTCCTGGTCT GAATGGCTTA CATGGTCTGA AGGGTGAGAA AGGAACAAAA 
GGCGCCTCAG GTTTGCTCGA AATGGGTCCA CCTGGGCCAA TGGGGATGCC TGGGCAAAAA 
GGAGAGAAAG GAGACCCTGG GAGTCCAGGG ATTTCTCCTC CAGGTCTTCC TGGAGAAAAG 
GGCTTCCCAG GACCCCCAGG GAGACCAGGA CCACCTGGTC CCGCAGGTGC CCCAGGAAGA 
GCTGCTAAAG GTGACATTCC TGATCCAGGT CCACCTGGAG ACCGGGGACC TCCTGGCCCC 
GATGGCCCAA GAGGAGTACC TGGGCCTCCG GGTTCCCCTG GGAATGTTGA CCTTCTGAAA 
GGGGACCCAG GTGACTGTGG TTTGCCAGGA CCACCTGGCT CCCGAGGCCC ACCAGGCCCT 
CCAGGGTGTC AGGGTCCCCC AGGATGTGAT GGCAAAGATG GCCAGAAAGG ACCAATGGGA 
CTTCCAGGGT TGCCAGGGCC ACCTGGTCTT CCTGGGGCAC CTGGCGAGAA GGGATTACCT 
GGCCCTCCAG GCAGAAAAGG GCCAGTAGGT CCTCCAGGCT GCAGAGGTGA ACCTGGGCCT 
CCTGCAGACG TGGATTCCTG TCCTCGAATC CCAGGACTTC CTGGAGTGCC AGGTCCCAGA 
GGACCAGAAG GAGCTATGGG GGAACCTGGA CGGAGAGGCC TGCCTGGACC AGGGTGCAAA 
GGAGAGCCTG GACCGGATGG CAGACGGGGC CAGGATGGCA TCCCTGGATC TCCCGGGCCT 
CCTGGACGCA AAGGTGACAC TGGAGAGGCA GGGTGTCCTG GAGCACCAGG TTTCTTACTG 
AGTCTGGAGA TTGCCAACTG GCAAGGCTGC CCCAGCAAGG AGCTGCAAGC ATCCTCCCGT 
CTCCATGCCG CCAACACTGG GTTCTGGATT CAGTGGGCTT TATTGGGGTT CCTGGGGATC 
TGGACTCCTT GTTTTACCGA AAGTGATACC AGCCCTATGG GGGTCATTGG TATACTGCAT 
CATTTAGGGA AACTGTCAGG GAACGGAGAC TACATGCTTA ATTCAGAAGC AATTAAAAAT 
TATATATTTT TCAGTTCTCA GTTGAGTGAT CAGCCTTGCA GAAGCACAGA GGCTTTGCTG 
GAGGAGTTGT GTTTTTGTGT ACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236955.1
CDS	430..5076
Translation	MRCFFRWTKSFVTAPWSLIFILFTIQYEYGSGKKYGGPCGGRNCSVCQCFPEKGSRGHPGPLGPQGPIGPLGPLGPIGIPGEKGERGDSGSPGPPGEKGDKGPTGVPGFPGVDGVPGHPGPPGPRGKPGVDGYNGSRGDPGYPGERGAPGPGGPPGQPGENGEKGRSVYITGGVKGIQGDRGDPGPPGLPGSRGAQGSPGPMGHAGAPGLAGPIGHPGSPGLKGNPATGLKGQRGEPGEVGQRGPPGPTLLVQPPDLSIYKGEKGVKGMPGMIGPPGPPGRKGAPGVGIKGEKGIPGFPGPRGEPGSHGPPGFPGFKGIQGAAGEPGLFGFLGPKGDLGDRGYPGPPGILLTPAPPLKGVPGDPGPPGYYGEIGDVGLPGPPGPPGRPGETCPGMMGPPGPPGVPGPPGFPGEAGVPGRLDCAPGKPGKPGLPGLPGAPGPEGPPGSDVIYCRPGCPGPMGEKGKVGPPGRRGAKGAKGNKGLCTCPPGPMGPPGPPGPPGRQGSKGDLGLPGWHGEKGDPGQPGAEGPPGPPGRPGAMGPPGHKGEKGDMVISRVKGQKGERGLDGPPGFPGPHGQDGGDGRPGERGDPGPRGDHKDAAPGERGLPGLPGPPGRTGPEGPPGLGFPGPPGQRGLPGEPGRPGTRGFDGTKGQKGDSILCNVSYPGKPGLPGLDGPPGLKGFPGPPGAPGMRCPDGQKGQRGKPGMSGIPGPPGFRGDMGDPGIKGEKGTSPIGPPGPPGSPGKDGQKGIPGDPAFGDPGPPGERGLPGAPGMKGQKGHPGCPGAGGPPGIPGSPGLKGPKGREGSRGFPGIPGSPGHSCERGAPGIPGQPGLPGTPGDPGAPGWKGQPGDMGPSGPAGMKGLPGLPGLPGADGLRGPPGIPGPNGEDGLPGLPGLKGLPGLPGFPGFPGERGKPGPDGEPGRKGEVGEKGWPGLKGDLGERGAKGDRGLPGDAGEAVTSRKGEPGDAGPPGDGGFSGERGDKGSSGMRGGRGDPGRDGLPGLHRGQPGIDGPPGPPGPPGPPGSPGLRGVIGFPGFPGDQGDPGSPGPPGFPGDDGARGPKGYKGDPASQCGPPGPKGEPGSPGYQGRTGVPGEKGFPGDEGPRGPPGRPGQPGSFGPPGCPGDPGMPGLKGHPGEVGDPGPRGDAGDFGRPGPAGVKGPLGSPGLNGLHGLKGEKGTKGASGLLEMGPPGPMGMPGQKGEKGDPGSPGISPPGLPGEKGFPGPPGRPGPPGPAGAPGRAAKGDIPDPGPPGDRGPPGPDGPRGVPGPPGSPGNVDLLKGDPGDCGLPGPPGSRGPPGPPGCQGPPGCDGKDGQKGPMGLPGLPGPPGLPGAPGEKGLPGPPGRKGPVGPPGCRGEPGPPADVDSCPRIPGLPGVPGPRGPEGAMGEPGRRGLPGPGCKGEPGPDGRRGQDGIPGSPGPPGRKGDTGEAGCPGAPGFLLSLEIANWQGCPSKELQASSRLHAANTGFWIQWALLGFLGIWTPCFTESDTSPMGVIGILHHLGKLSGNGDYMLNSEAIKNYIFFSSQLSDQPCRSTEALLEELCFCVL

Target ORF information:

RefSeq Version	XM_011238653.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 4 (Col4a4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238653.2

ORF Insert Sequence:

ATGAGGTGCT TTTTCAGATG GACCAAGTCC TTTGTCACAG CCCCCTGGTC ACTAATATTT 
ATCCTCTTTA CTATACAATA TGAATATGGG AGTGGCAAGA AGTATGGCGG CCCCTGCGGA 
GGAAGAAACT GCTCTGTCTG CCAGTGTTTT CCTGAGAAGG GATCTCGGGG TCACCCAGGA 
CCACTGGGAC CACAGGGACC AATAGGGCCC TTGGGACCCC TGGGACCTAT TGGGATTCCA 
GGAGAAAAAG GAGAGAGAGG AGACAGTGGC TCACCTGGAC CACCAGGAGA GAAAGGAGAC 
AAGGGTCCAA CTGGTGTCCC TGGATTTCCA GGTGTGGATG GTGTACCTGG TCACCCAGGG 
CCTCCTGGAC CCAGAGGCAA ACCTGGTGTG GACGGCTACA ATGGCTCACG TGGTGATCCG 
GGCTATCCAG GAGAAAGAGG AGCTCCAGGC CCAGGCGGCC CCCCTGGTCA GCCTGGGGAA 
AATGGAGAAA AAGGAAGATC TGTGTACATT ACTGGTGGCG TTAAAGGTAT TCAGGGAGAC 
CGTGGGGACC CAGGACCACC CGGCTTACCA GGATCTAGGG GTGCACAAGG GTCACCAGGG 
CCCATGGGGC ATGCAGGCGC ACCAGGGCTA GCAGGACCTA TAGGTCATCC TGGAAGCCCT 
GGGTTGAAGG GAAATCCTGC CACTGGACTG AAGGGACAAA GGGGAGAGCC GGGTGAAGTT 
GGCCAGCGTG GTCCTCCTGG GCCCACGCTG TTGGTACAGC CGCCTGATTT GAGTATCTAT 
AAAGGAGAAA AGGGTGTAAA AGGAATGCCT GGAATGATTG GTCCCCCAGG ACCTCCAGGT 
CGCAAGGGAG CGCCTGGTGT CGGGATCAAA GGAGAGAAAG GTATCCCTGG GTTCCCAGGA 
CCCCGGGGTG AGCCTGGTTC GCATGGGCCT CCAGGTTTTC CAGGATTCAA GGGGATACAA 
GGAGCAGCTG GAGAGCCTGG GCTGTTCGGA TTTCTTGGTC CAAAGGGGGA TCTGGGAGAT 
CGCGGGTACC CGGGACCACC AGGCATTTTG TTGACTCCAG CTCCACCACT CAAAGGTGTT 
CCAGGGGACC CAGGACCCCC TGGCTACTAT GGGGAGATTG GAGATGTTGG ATTACCAGGT 
CCTCCAGGGC CCCCAGGCAG ACCAGGGGAA ACTTGTCCAG GCATGATGGG ACCTCCTGGA 
CCACCAGGGG TTCCTGGTCC TCCAGGATTT CCAGGAGAAG CTGGGGTTCC TGGAAGACTC 
GACTGTGCCC CAGGAAAACC TGGGAAGCCA GGGCTACCCG GCCTGCCTGG CGCACCAGGA 
CCAGAGGGGC CCCCTGGATC AGATGTGATA TACTGTCGCC CTGGGTGCCC TGGGCCAATG 
GGTGAAAAGG GAAAAGTGGG TCCTCCAGGA AGAAGAGGAG CCAAAGGAGC AAAAGGAAAC 
AAGGGACTCT GTACCTGTCC ACCTGGTCCC ATGGGACCCC CTGGTCCCCC AGGACCTCCT 
GGAAGACAAG GAAGTAAAGG AGACTTAGGG CTCCCTGGTT GGCATGGAGA AAAGGGCGAT 
CCAGGTCAGC CTGGTGCTGA AGGACCGCCA GGGCCACCAG GAAGACCTGG TGCCATGGGG 
CCCCCTGGTC ACAAAGGGGA AAAGGGTGAC ATGGTCATAT CAAGAGTGAA AGGGCAAAAA 
GGAGAAAGAG GACTGGATGG GCCACCAGGA TTTCCGGGGC CACATGGACA AGATGGTGGG 
GATGGACGTC CTGGAGAAAG AGGGGATCCT GGGCCACGAG GGGACCATAA GGATGCAGCC 
CCGGGTGAGA GAGGGCTTCC CGGACTGCCA GGCCCTCCAG GAAGAACAGG ACCTGAGGGG 
CCTCCAGGCC TGGGATTTCC AGGCCCACCA GGACAGCGAG GCCTACCCGG AGAGCCCGGG 
CGTCCGGGCA CCAGAGGCTT TGATGGCACG AAGGGGCAGA AAGGTGACTC CATTCTGTGT 
AATGTAAGCT ACCCGGGAAA ACCAGGTCTG CCAGGTCTTG ATGGACCTCC AGGTCTGAAG 
GGGTTTCCAG GCCCCCCAGG GGCTCCTGGT ATGAGATGTC CAGATGGGCA GAAAGGCCAG 
CGGGGCAAAC CGGGAATGTC TGGGATTCCA GGTCCACCTG GTTTTCGTGG TGACATGGGA 
GATCCAGGCA TCAAAGGTGA AAAGGGGACC TCCCCTATTG GACCCCCAGG CCCACCTGGG 
TCTCCTGGAA AGGATGGCCA GAAAGGAATC CCAGGAGACC CTGCATTTGG TGACCCAGGA 
CCCCCTGGAG AGAGGGGTCT TCCAGGAGCA CCAGGCATGA AAGGACAGAA AGGTCACCCA 
GGGTGCCCAG GGGCTGGAGG CCCTCCTGGC ATCCCTGGAT CTCCAGGTCT CAAAGGTCCC 
AAAGGTAGAG AGGGAAGTCG TGGCTTTCCA GGGATCCCAG GATCCCCTGG CCATTCCTGT 
GAAAGAGGTG CTCCCGGGAT CCCAGGACAA CCGGGACTTC CTGGAACTCC AGGTGATCCA 
GGGGCCCCAG GTTGGAAAGG CCAGCCAGGA GACATGGGTC CCTCTGGACC AGCTGGGATG 
AAGGGCCTCC CTGGTCTCCC AGGCCTACCA GGAGCTGATG GTCTCCGAGG GCCTCCTGGG 
ATTCCAGGCC CCAATGGGGA AGATGGTCTA CCTGGTCTTC CAGGCCTGAA GGGACTCCCA 
GGGCTGCCTG GCTTCCCTGG TTTTCCAGGA GAGAGAGGAA AGCCAGGGCC AGATGGAGAA 
CCTGGAAGAA AGGGAGAGGT TGGAGAGAAA GGCTGGCCTG GCTTGAAGGG AGACCTGGGA 
GAGCGAGGCG CCAAAGGAGA CAGAGGACTT CCTGGTGATG CAGGAGAAGC AGTCACTTCC 
AGAAAGGGTG AACCTGGGGA TGCTGGGCCT CCAGGAGATG GTGGGTTCTC AGGAGAGAGA 
GGTGATAAAG GAAGCTCAGG GATGCGAGGG GGAAGAGGGG ATCCCGGAAG AGATGGACTA 
CCGGGACTCC ACAGAGGGCA GCCTGGGATA GATGGGCCTC CTGGGCCTCC TGGGCCCCCT 
GGCCCTCCAG GATCACCTGG ACTGAGAGGA GTCATTGGTT TCCCAGGATT TCCAGGTGAC 
CAGGGTGATC CAGGTTCTCC AGGACCCCCT GGATTCCCAG GAGATGATGG AGCAAGAGGA 
CCTAAAGGAT ACAAAGGTGA CCCTGCCAGT CAGTGTGGTC CACCTGGTCC AAAGGGTGAG 
CCAGGTAGTC CTGGATACCA AGGACGTACT GGAGTCCCCG GAGAGAAAGG CTTTCCTGGA 
GATGAAGGAC CCCGAGGACC ACCAGGCAGA CCTGGACAGC CTGGATCTTT TGGACCACCA 
GGGTGTCCAG GTGACCCAGG GATGCCCGGA CTCAAGGGAC ATCCAGGAGA AGTGGGAGAC 
CCTGGGCCAA GAGGTGATGC GGGAGATTTC GGGAGGCCAG GTCCTGCTGG AGTGAAAGGG 
CCTCTCGGGT CTCCTGGTCT GAATGGCTTA CATGGTCTGA AGGGTGAGAA AGGAACAAAA 
GGCGCCTCAG GTTTGCTCGA AATGGGTCCA CCTGGGCCAA TGGGGATGCC TGGGCAAAAA 
GGAGAGAAAG GAGACCCTGG GAGTCCAGGG ATTTCTCCTC CAGGTCTTCC TGGAGAAAAG 
GGCTTCCCAG GACCCCCAGG GAGACCAGGA CCACCTGGTC CCGCAGGTGC CCCAGGAAGA 
GCTGCTAAAG GTGACATTCC TGATCCAGGT CCACCTGGAG ACCGGGGACC TCCTGGCCCC 
GATGGCCCAA GAGGAGTACC TGGGCCTCCG GGTTCCCCTG GGAATGTTGA CCTTCTGAAA 
GGGGACCCAG GTGACTGTGG TTTGCCAGGA CCACCTGGCT CCCGAGGCCC ACCAGGCCCT 
CCAGGGTGTC AGGGTCCCCC AGGATGTGAT GGCAAAGATG GCCAGAAAGG ACCAATGGGA 
CTTCCAGGGT TGCCAGGGCC ACCTGGTCTT CCTGGGGCAC CTGGCGAGAA GGGATTACCT 
GGCCCTCCAG GCAGAAAAGG GCCAGTAGGT CCTCCAGGCT GCAGAGGTGA ACCTGGGCCT 
CCTGCAGACG TGGATTCCTG TCCTCGAATC CCAGGACTTC CTGGAGTGCC AGGTCCCAGA 
GGACCAGAAG GAGCTATGGG GGAACCTGGA CGGAGAGGCC TGCCTGGACC AGGGTGCAAA 
GGAGAGCCTG GACCGGATGG CAGACGGGGC CAGGATGGCA TCCCTGGATC TCCCGGGCCT 
CCTGGACGCA AAGGTGACAC TGGAGAGGCA GGGTGTCCTG GAGCACCAGG TTTCTTACTG 
AGTCTGGAGA TTGCCAACTG GCAAGGCTGC CCCAGCAAGG AGCTGCAAGC ATCCTCCCGT 
CTCCATGCCG CCAACACTGG GTTCTGGATT CAGTGGGCTT TATTGGGGTT CCTGGGGATC 
TGGACTCCTT GTTTTACCGA AAGTGATACC AGCCCTATGG GGGTCATTGG TATACTGCAT 
CATTTAGGGA AACTGTCAGG GAACGGAGAC TACATGCTTA ATTCAGAAGC AATTAAAAAT 
TATATATTTT TCAGTTCTCA GTTGAGTGAT CAGCCTTGCA GAAGCACAGA GGCTTTGCTG 
GAGGAGTTGT GTTTTTGTGT ACTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

PDGFR? regulates craniofacial development through homodimers and functional heterodimers with PDGFR?.
Genes & development30(21)2443-2458(2016 Nov)
Fantauzzo KA,Soriano P

Thyroid follicle development requires Smad1/5- and endothelial cell-dependent basement membrane assembly.
Development (Cambridge, England)143(11)1958-70(2016 06)
Villacorte M,Delmarcelle AS,Lernoux M,Bouquet M,Lemoine P,Bols?e J,Umans L,de Sousa Lopes SC,Van Der Smissen P,Sasaki T,Bommer G,Henriet P,Refetoff S,Lemaigre FP,Zwijsen A,Courtoy PJ,Pierreux CE

A mouse Col4a4 mutation causing Alport glomerulosclerosis with abnormal collagen ?3?4?5(IV) trimers.
Kidney international85(6)1461-8(2014 Jun)
Korstanje R,Caputo CR,Doty RA,Cook SA,Bronson RT,Davisson MT,Miner JH

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Col4a4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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