Col4a3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col4a3
Entrez Gene ID	12828
Full Name	collagen, type IV, alpha 3
Synonyms	[a]3(IV),alpha3(IV)
General protein information	Preferred Names collagen, type IV, alpha 3 Names collagen alpha-3(IV) chain col4a3 collagen type IV alpha3 chain procollagen, type IV, alpha 3 tumstatin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_007734.2	NP_031760.2	collagen alpha-3(IV) chain precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A3	XP_516130.2
Canis lupus familiaris (dog)	COL4A3	XP_005635825.1
Bos taurus (cattle)	COL4A3	NP_001160001.1
Rattus norvegicus (Norway rat)	Col4a3	NP_001129231.1
Mus musculus (house mouse)	Col4a3	NP_031760.2
	COL4A3	XP_001101448.2
Gallus gallus (chicken)	COL4A3	XP_001234730.3
Homo sapiens (human)	COL4A3	NP_000082.2

Gene Ontology
Bibliography

Related articles in PubMed

Pathogenicity of a Human Laminin
Funk SD, Bayer RH, Malone AF, McKee KK, Yurchenco PD, Miner JH
Journal of the American Society of Nephrology : JASN(2017 Dec)

Ultrastructural Characterization of the Glomerulopathy in Alport Mice by Helium Ion Scanning Microscopy (HIM).
Tsuji K, Suleiman H, Miner JH, Daley JM, Capen DE, P?unescu TG, Lu HAJ
Scientific reports7(1)11696(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The quality and quantity of alpha3IV-NC1-specific Ab and T cell responses are critical for the phenotype of the glomerulonephritis.
Title: Glomerulopathy induced by immunization with a peptide derived from the goodpasture antigen ?3IV-NC1.

Report novel mouse model of Alport syndrome with accumulation abnormal collagen alpha3alpha4alpha5(IV) trimers in the glomerular basement membrane.
Title: A mouse Col4a4 mutation causing Alport glomerulosclerosis with abnormal collagen ?3?4?5(IV) trimers.

Report monoclonal antibody against the collagen type IV alpha3NC1 domain as a marker for glomerular disease.
Title: A human monoclonal antibody against the collagen type IV ?3NC1 domain is a non-invasive optical biomarker for glomerular diseases.

Data show that deletion of tumstatin and TSP1 in p53-/- mice leads to increased tumor burden and reduced survival.
Title: Counterbalancing angiogenic regulatory factors control the rate of cancer progression and survival in a stage-specific manner.

the pathogenetic role of USAG-1 in Col4a3-/- mice might involve crosstalk between kidney tubules and the glomerulus and that inhibition of USAG-1 may be a promising therapeutic approach for the treatment of Alport syndrome.
Title: Loss of the BMP antagonist USAG-1 ameliorates disease in a mouse model of the progressive hereditary kidney disease Alport syndrome.

The following Col4a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col4a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22190	NM_007734.2 Latest version!	Mus musculus collagen, type IV, alpha 3 (Col4a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col4a3 ( NM_007734.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031760.2 Mus musculus collagen, type IV, alpha 3 (Col4a3), mRNA.

Price & Availability↑

CloneID	OMu22190
Clone ID Related Accession (Same CDS sequence)	NM_007734.2
Accession Version	NM_007734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	collagen alpha-3(IV) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB605811.2, AF169387.1 and AC138214.8. On Apr 25, 2007 this sequence version replaced NM_007734.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF169387.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGCACTCCA AGACTGCTCC AAGGTTCCTG GTGTTCCTGC TGCTTACCCT GCTGCTACTC 
CTGGCTGCCT CTCCTGTGGC TAGCAAGGGC TGTGTCTGCA AAGGCAAAGG ACAATGCCTC 
TGCGCGGGGA CCAAAGGGGA GAAGGGGGAG AAAGGGGTTC CTGGTTCCCC TGGATTTCCT 
GGCCAGAAAG GATTTCCAGG TCCTGAAGGC TTGCCTGGAC CACAGGGACC CAAGGGCTCC 
CCAGGACTTC CGGGACTCAC TGGCCCCAAA GGCATCAGGG GAATAACTGG ATTACCAGGG 
TTTGCAGGTC CTCCTGGACT TCCAGGCCTC CCAGGCCACC CTGGGCCTCG TGGGCTGGCT 
GGTTTACCAG GATGCAACGG ATCTAAGGGT GAACAAGGAT TCCCGGGCTT TCCCGGCACA 
CCAGGCTATG CAGGGCTCCC AGGTCCTGAT GGCTTGAAAG GACAAAAGGG TGAGCCTGCT 
CAAGGAGAAG ACAGGGGATT CAATGGAAAA GGTGACCCTG GGCCTCCAGG GGTTCCAGGC 
TTCCAGGGTT TTCCGGGACT CCCAGGTTTT CCAGGGCCTG CCGGTCCACC AGGACCTCCG 
GGATTTTTTG GTTTACCAGG AGCAATGGGA CCCAGAGGAC CCAAAGGCCA CATGGGCGAT 
AGCGTGATAG GACAAAAAGG AGAAAGGGGT ATGAAAGGAT TAACAGGACC TCCTGGGCCA 
CCAGGAACAG TGATTTTTAC ACTCACCCAG CCATACAACA AATCGGACTT CAAGGGAGAG 
AAAGGAGATG AGGGAGAGAG GGGTGAACCC GGACCTCCTG GACCCTCGGG GCCACCTGGA 
GACTCCTATG GATCAGAAAA GGGTGCGCCT GGAGAGCCTG GTCCTCGGGG CAAACCTGGA 
AAAGATGGTG CCCCTGGCTT CCCTGGCACT GAGGGAGCCA AAGGCAACAG GGGCTTTCCT 
GGGTTGAGGG GTGAAGCTGG CATTAAGGGA AGGAAAGGAG ACATTGGCCC CCCAGGATTT 
CCTGGTCCAA CAGAATATTA TGACGCATAC CTGGAAAAGG GAGAGAGAGG AATGCCAGGC 
CTTCCTGGGC CCAAAGGAGC CCGTGGCCCA CAGGGTCCAA GTGGTCCCCC TGGAGTCCCT 
GGAAGTCCTG GACTGTCAAG GCCTGGCCTC AGAGGACCCA TTGGATGGCC AGGCTTGAAA 
GGGAGTAAAG GAGAGAGAGG ACCCCCTGGA AAAGACACTG TGGGCCCTCC TGGACCCCTG 
GGATGTCCTG GCTCACCAGG TCCACCAGGC CCTCCAGGAC CTCCAGGATG TCCAGGTGAC 
ATTGTTTTTA AATGTTCCCC TGGTGAGCAT GGAATGCCAG GTGACACAGG ACCTCCAGGA 
GTTCCAGGAC TTGATGGACC CAAAGGAGAA CCAGGCAGTC CATGCACCGA GTGTCACTGC 
TTCCCAGGGC CCCCTGGAGT CCCAGGATTT CCAGGATTAG ATGGTATCAA AGGAATCCCA 
GGAGGACGAG GTGTGCCTGG TCTGAAGGGA AACCCAGGGT CCCCAGGAAG TGCAGGTCTC 
CCAGGATTTG CAGGATTCCC AGGTGACCAA GGGCATCCAG GACTTAAAGG GGACAAAGGG 
GATACACCTC TACCCTGGGG GCAAGTGGGT AATCCAGGTG ATCCTGGACT CAGAGGCCTG 
CCCGGGAGAA AGGGCTTCGA TGGAACTCCT GGAGGTCCAG GAGCGAAAGG ACCACCAGGA 
CCTCAGGGCG AACCGGCCCT GAGTGGAAGG AAAGGGGACC AAGGACCTCC AGGACCTCCT 
GGATTCCCTG GACCCCCAGG ACCTGCAGGA CCAGCTGGAC CACCAGGCTA TGGACCTCAA 
GGAGAGCCAG GTCCAAAGGG AGCCCAAGGA GTCCCCGGCG TCCTGGGACC ACCTGGAGAA 
GCCGGTCTTA AAGGAGAACC CAGTACATCA ACTCCAGATC TAGGTCCCCC AGGCCCTCCA 
GGGCCCCCTG GTCAGGCTGG CCCCAGAGGT CTACCTGGTT TGCCTGGACC GGTGGGAAAA 
TGTGATCCTG GTCTTCCTGG ACCTGATGGT GAGCCAGGAA TTCCAGAAGC TGGATGCCCA 
GGGCCTCCAG GACCTAAGGG AAATCAAGGT TTCCCAGGGA CAAAAGGATC ACCTGGTTGT 
CCTGGGGAAA TGGGAAAGCC TGGCCGTCCT GGAGAACCAG GCATCCCAGG AGCCAAGGGA 
GAACCATCCG TAGGCAGGCC TGGGAAACCA GGGAAACCAG GCTTTCCAGG AGAAAGAGGC 
AATGCTGGGG AAAATGGAGA TATTGGACTC CCTGGGCTTC CAGGCCTCCC TGGAACTCCA 
GGAAGAGGCG GGCTTGACGG GCCTCCAGGA GACCCAGGAC AGCCTGGATC ACCTGGAGCA 
AAAGGATCCC CAGGAAGGTG CATACCAGGA CCCAGGGGCA CCCAAGGACT TCCCGGCTTG 
AATGGATTGA AAGGGCAACC AGGTCGAAGA GGTGATACCG GGCCAAAGGG AGACCCTGGC 
ATCCCAGGCA TGGACAGGTC TGGGGTCCCC GGAGATCCTG GACCACCAGG AACACCAGGT 
TGTCCTGGTG AGATGGGACC ACCTGGACAA AAAGGATATC CGGGAGCTCC AGGATTCCCA 
GGACCACCAG GCGAGAAAGG AGAGGTTGGA ATGATGGGCT ATCCTGGAAC CACTGGCCCT 
CCGGGGCTTC CTGGGAAACC AGGCTCACAG GGGCAGCGAG GTAGCCTCGG AATCCCAGGA 
ATGAAAGGGG AAAAAGGACG CCCAGGAGCC AAAGGCGAAC GAGGAGAGAA AGGAAAACCA 
GGGCCTTCTC AAACAACACT CTTAAAGGGA GACAAAGGAG AGCCTGGACT TAAAGGATTT 
GTTGGGAATC CAGGTGAGAA AGGAAACAGA GGCAACCCAG GGTTACCAGG TCCGAAAGGC 
CTCGAGGGAT TGCCTGGGCT ACCAGGTCCT CCAGGCCCTA GAGGAGATAC GGGAAGCAGA 
GGAAATCCTG GAAGACCAGG ACCACACGGC ATGCCAGGAA GCATGGGGAT CATGGGGGTG 
CCAGGCCCTA AAGGGAGAAA AGGAACTTCA GGGCTTCCAG GTCTAGCTGG AAGACCAGGC 
CTGACAGGGA TCCATGGTCC CCAAGGAGAT AAGGGGGAGC CGGGTTATTC AGAAGGTGCA 
AGACCAGGAC CACCAGGACC AAAGGGCGAT CCAGGATTGC CAGGTGACAA AGGAAAGAAA 
GGAGAAAGAG GCGTACCTGG GCCACCTGGA CAATCGGGGC CTGCCGGACC TGATGGAGCC 
CCTGGGAGTC CTGGGAGTCC TGGTCACCCA GGAAAACCAG GTCCTGCTGG TGATTTGGGT 
CTGAAAGGAC AGAAAGGCTT CCCAGGCCCT CCAGGAAGCA CTGGCCCTCC AGGCCCTCCA 
GGACTCCCAG GACTTCCTGG GCCAATGGGT ATGAGAGGTG ACCAAGGACG TGATGGAATT 
CCTGGTCCTC CAGGAGAAAA AGGAGAAACA GGCTTGCTGG GGGCCTACCC AGGCCCAAAA 
GGGAGCCCTG GTGTACCAGG TGCCAAAGGA GACAGGGGAG TTCCAGGCTT GTCTGGCCTT 
CCCGGCAGGA AGGGGGTTAT GGGGGATGTT GGACCCCAAG GACCCCCAGG CACTGCTGGA 
CTCCCAGGGC CACCAGGTCT ACCTGGGGCA ATTATCCCTG GCCCCAAAGG AGACAGAGGT 
CTTCCTGGCT TAAGAGGAAA TCCAGGTGAG CCAGGTCCCC CTGGACCTCC AGGACCTATC 
GGAAAAGGCA TAAAAGGTGA CAAAGGATTT ATGGGCCCAC CTGGCCCCAA AGGCCTGCCT 
GGAACTGTAG GGGACATGGG CCCACCAGGT TTCCCGGGAG CACCAGGTAC CCCCGGTCTT 
CCCGGTGTCA GAGGTGATCC AGGATTCCCT GGATTTCCAG GCATAAAAGG AGAAAAGGGT 
AACCCTGGAT TTCTTGGGCC AATTGGACAT CCAGGACCAG TTGGGCCCAA AGGACCACCA 
GGTCCACGTG GAAAACCTGG CACTCTTAAG GTCATCTCTC TTCCGGGAAG CCCAGGGCCA 
CCTGGTGTAC CTGGACAGCC AGGAATGAAA GGAGACCCTG GACCCCTGGG ACTGCCAGGA 
ATCCCAGGAC CCTGTGGGCC AAGAGGTAAA CCAGGCAAGG ATGGGAAACC AGGAACTCCA 
GGACCAGCTG GTACAAAGGG CAACAAGGGC TTGAAAGGAC AGCAAGGCCC ACCTGGTCTG 
GATGGATTGC CAGGCTTAAA GGGAAATCCT GGTGACAGGG GAACGCCGGC CACTGGTACA 
AGAATGCGAG GCTTCATCTT CACCCGACAC AGTCAAACCA CGGCCATTCC TTCATGCCCT 
GAAGGAACAC AGCCACTCTA TAGTGGGTTT TCTCTTCTTT TTGTACAAGG AAACAAACGT 
GCACATGGAC AAGACCTAGG TACTCTGGGC AGCTGCCTGC AGCGATTCAC CACAATGCCG 
TTCTTATTCT GTAACATCAA TAATGTATGT AACTTTGCAT CACGAAATGA TTATTCATAC 
TGGCTGTCAA CACCAGCTCT GATGCCAATG GACATGGCTC CAATTAGTGG CAGAGCTCTC 
GAACCCTATA TTAGCAGATG CACCGTCTGT GAAGGTCCAG CAATGGCCAT AGCTGTTCAC 
AGTCAAACTA CTGCTATCCC TCCGTGTCCC CAGGACTGGG TTTCTCTCTG GAAAGGTTTT 
TCTTTCATTA TGTTCACAAG TGCAGGCTCT GAGGGTGCTG GACAAGCACT TGCCTCGCCT 
GGCTCCTGCC TGGAAGAATT CCGAGCCAGT CCATTTATAG AATGCCATGG ACGAGGGACA 
TGTAACTACT ACTCAAACTC CTACAGTTTC TGGCTGGCTT CGCTGAACCC AGAAAGAATG 
TTCAGAAAAC CTATTCCATC AACTGTGAAA GCTGGAGACT TAGAGAAAAT CATAAGCCGC 
TGTCAGGTGT GCATGAAGAA AAGACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031760.2
CDS	425..5434
Translation	MHSKTAPRFLVFLLLTLLLLLAASPVASKGCVCKGKGQCLCAGTKGEKGEKGVPGSPGFPGQKGFPGPEGLPGPQGPKGSPGLPGLTGPKGIRGITGLPGFAGPPGLPGLPGHPGPRGLAGLPGCNGSKGEQGFPGFPGTPGYAGLPGPDGLKGQKGEPAQGEDRGFNGKGDPGPPGVPGFQGFPGLPGFPGPAGPPGPPGFFGLPGAMGPRGPKGHMGDSVIGQKGERGMKGLTGPPGPPGTVIFTLTQPYNKSDFKGEKGDEGERGEPGPPGPSGPPGDSYGSEKGAPGEPGPRGKPGKDGAPGFPGTEGAKGNRGFPGLRGEAGIKGRKGDIGPPGFPGPTEYYDAYLEKGERGMPGLPGPKGARGPQGPSGPPGVPGSPGLSRPGLRGPIGWPGLKGSKGERGPPGKDTVGPPGPLGCPGSPGPPGPPGPPGCPGDIVFKCSPGEHGMPGDTGPPGVPGLDGPKGEPGSPCTECHCFPGPPGVPGFPGLDGIKGIPGGRGVPGLKGNPGSPGSAGLPGFAGFPGDQGHPGLKGDKGDTPLPWGQVGNPGDPGLRGLPGRKGFDGTPGGPGAKGPPGPQGEPALSGRKGDQGPPGPPGFPGPPGPAGPAGPPGYGPQGEPGPKGAQGVPGVLGPPGEAGLKGEPSTSTPDLGPPGPPGPPGQAGPRGLPGLPGPVGKCDPGLPGPDGEPGIPEAGCPGPPGPKGNQGFPGTKGSPGCPGEMGKPGRPGEPGIPGAKGEPSVGRPGKPGKPGFPGERGNAGENGDIGLPGLPGLPGTPGRGGLDGPPGDPGQPGSPGAKGSPGRCIPGPRGTQGLPGLNGLKGQPGRRGDTGPKGDPGIPGMDRSGVPGDPGPPGTPGCPGEMGPPGQKGYPGAPGFPGPPGEKGEVGMMGYPGTTGPPGLPGKPGSQGQRGSLGIPGMKGEKGRPGAKGERGEKGKPGPSQTTLLKGDKGEPGLKGFVGNPGEKGNRGNPGLPGPKGLEGLPGLPGPPGPRGDTGSRGNPGRPGPHGMPGSMGIMGVPGPKGRKGTSGLPGLAGRPGLTGIHGPQGDKGEPGYSEGARPGPPGPKGDPGLPGDKGKKGERGVPGPPGQSGPAGPDGAPGSPGSPGHPGKPGPAGDLGLKGQKGFPGPPGSTGPPGPPGLPGLPGPMGMRGDQGRDGIPGPPGEKGETGLLGAYPGPKGSPGVPGAKGDRGVPGLSGLPGRKGVMGDVGPQGPPGTAGLPGPPGLPGAIIPGPKGDRGLPGLRGNPGEPGPPGPPGPIGKGIKGDKGFMGPPGPKGLPGTVGDMGPPGFPGAPGTPGLPGVRGDPGFPGFPGIKGEKGNPGFLGPIGHPGPVGPKGPPGPRGKPGTLKVISLPGSPGPPGVPGQPGMKGDPGPLGLPGIPGPCGPRGKPGKDGKPGTPGPAGTKGNKGLKGQQGPPGLDGLPGLKGNPGDRGTPATGTRMRGFIFTRHSQTTAIPSCPEGTQPLYSGFSLLFVQGNKRAHGQDLGTLGSCLQRFTTMPFLFCNINNVCNFASRNDYSYWLSTPALMPMDMAPISGRALEPYISRCTVCEGPAMAIAVHSQTTAIPPCPQDWVSLWKGFSFIMFTSAGSEGAGQALASPGSCLEEFRASPFIECHGRGTCNYYSNSYSFWLASLNPERMFRKPIPSTVKAGDLEKIISRCQVCMKKRH

Target ORF information:

RefSeq Version	NM_007734.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 3 (Col4a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007734.2

ORF Insert Sequence:

ATGCACTCCA AGACTGCTCC AAGGTTCCTG GTGTTCCTGC TGCTTACCCT GCTGCTACTC 
CTGGCTGCCT CTCCTGTGGC TAGCAAGGGC TGTGTCTGCA AAGGCAAAGG ACAATGCCTC 
TGCGCGGGGA CCAAAGGGGA GAAGGGGGAG AAAGGGGTTC CTGGTTCCCC TGGATTTCCT 
GGCCAGAAAG GATTTCCAGG TCCTGAAGGC TTGCCTGGAC CACAGGGACC CAAGGGCTCC 
CCAGGACTTC CGGGACTCAC TGGCCCCAAA GGCATCAGGG GAATAACTGG ATTACCAGGG 
TTTGCAGGTC CTCCTGGACT TCCAGGCCTC CCAGGCCACC CTGGGCCTCG TGGGCTGGCT 
GGTTTACCAG GATGCAACGG ATCTAAGGGT GAACAAGGAT TCCCGGGCTT TCCCGGCACA 
CCAGGCTATG CAGGGCTCCC AGGTCCTGAT GGCTTGAAAG GACAAAAGGG TGAGCCTGCT 
CAAGGAGAAG ACAGGGGATT CAATGGAAAA GGTGACCCTG GGCCTCCAGG GGTTCCAGGC 
TTCCAGGGTT TTCCGGGACT CCCAGGTTTT CCAGGGCCTG CCGGTCCACC AGGACCTCCG 
GGATTTTTTG GTTTACCAGG AGCAATGGGA CCCAGAGGAC CCAAAGGCCA CATGGGCGAT 
AGCGTGATAG GACAAAAAGG AGAAAGGGGT ATGAAAGGAT TAACAGGACC TCCTGGGCCA 
CCAGGAACAG TGATTTTTAC ACTCACCCAG CCATACAACA AATCGGACTT CAAGGGAGAG 
AAAGGAGATG AGGGAGAGAG GGGTGAACCC GGACCTCCTG GACCCTCGGG GCCACCTGGA 
GACTCCTATG GATCAGAAAA GGGTGCGCCT GGAGAGCCTG GTCCTCGGGG CAAACCTGGA 
AAAGATGGTG CCCCTGGCTT CCCTGGCACT GAGGGAGCCA AAGGCAACAG GGGCTTTCCT 
GGGTTGAGGG GTGAAGCTGG CATTAAGGGA AGGAAAGGAG ACATTGGCCC CCCAGGATTT 
CCTGGTCCAA CAGAATATTA TGACGCATAC CTGGAAAAGG GAGAGAGAGG AATGCCAGGC 
CTTCCTGGGC CCAAAGGAGC CCGTGGCCCA CAGGGTCCAA GTGGTCCCCC TGGAGTCCCT 
GGAAGTCCTG GACTGTCAAG GCCTGGCCTC AGAGGACCCA TTGGATGGCC AGGCTTGAAA 
GGGAGTAAAG GAGAGAGAGG ACCCCCTGGA AAAGACACTG TGGGCCCTCC TGGACCCCTG 
GGATGTCCTG GCTCACCAGG TCCACCAGGC CCTCCAGGAC CTCCAGGATG TCCAGGTGAC 
ATTGTTTTTA AATGTTCCCC TGGTGAGCAT GGAATGCCAG GTGACACAGG ACCTCCAGGA 
GTTCCAGGAC TTGATGGACC CAAAGGAGAA CCAGGCAGTC CATGCACCGA GTGTCACTGC 
TTCCCAGGGC CCCCTGGAGT CCCAGGATTT CCAGGATTAG ATGGTATCAA AGGAATCCCA 
GGAGGACGAG GTGTGCCTGG TCTGAAGGGA AACCCAGGGT CCCCAGGAAG TGCAGGTCTC 
CCAGGATTTG CAGGATTCCC AGGTGACCAA GGGCATCCAG GACTTAAAGG GGACAAAGGG 
GATACACCTC TACCCTGGGG GCAAGTGGGT AATCCAGGTG ATCCTGGACT CAGAGGCCTG 
CCCGGGAGAA AGGGCTTCGA TGGAACTCCT GGAGGTCCAG GAGCGAAAGG ACCACCAGGA 
CCTCAGGGCG AACCGGCCCT GAGTGGAAGG AAAGGGGACC AAGGACCTCC AGGACCTCCT 
GGATTCCCTG GACCCCCAGG ACCTGCAGGA CCAGCTGGAC CACCAGGCTA TGGACCTCAA 
GGAGAGCCAG GTCCAAAGGG AGCCCAAGGA GTCCCCGGCG TCCTGGGACC ACCTGGAGAA 
GCCGGTCTTA AAGGAGAACC CAGTACATCA ACTCCAGATC TAGGTCCCCC AGGCCCTCCA 
GGGCCCCCTG GTCAGGCTGG CCCCAGAGGT CTACCTGGTT TGCCTGGACC GGTGGGAAAA 
TGTGATCCTG GTCTTCCTGG ACCTGATGGT GAGCCAGGAA TTCCAGAAGC TGGATGCCCA 
GGGCCTCCAG GACCTAAGGG AAATCAAGGT TTCCCAGGGA CAAAAGGATC ACCTGGTTGT 
CCTGGGGAAA TGGGAAAGCC TGGCCGTCCT GGAGAACCAG GCATCCCAGG AGCCAAGGGA 
GAACCATCCG TAGGCAGGCC TGGGAAACCA GGGAAACCAG GCTTTCCAGG AGAAAGAGGC 
AATGCTGGGG AAAATGGAGA TATTGGACTC CCTGGGCTTC CAGGCCTCCC TGGAACTCCA 
GGAAGAGGCG GGCTTGACGG GCCTCCAGGA GACCCAGGAC AGCCTGGATC ACCTGGAGCA 
AAAGGATCCC CAGGAAGGTG CATACCAGGA CCCAGGGGCA CCCAAGGACT TCCCGGCTTG 
AATGGATTGA AAGGGCAACC AGGTCGAAGA GGTGATACCG GGCCAAAGGG AGACCCTGGC 
ATCCCAGGCA TGGACAGGTC TGGGGTCCCC GGAGATCCTG GACCACCAGG AACACCAGGT 
TGTCCTGGTG AGATGGGACC ACCTGGACAA AAAGGATATC CGGGAGCTCC AGGATTCCCA 
GGACCACCAG GCGAGAAAGG AGAGGTTGGA ATGATGGGCT ATCCTGGAAC CACTGGCCCT 
CCGGGGCTTC CTGGGAAACC AGGCTCACAG GGGCAGCGAG GTAGCCTCGG AATCCCAGGA 
ATGAAAGGGG AAAAAGGACG CCCAGGAGCC AAAGGCGAAC GAGGAGAGAA AGGAAAACCA 
GGGCCTTCTC AAACAACACT CTTAAAGGGA GACAAAGGAG AGCCTGGACT TAAAGGATTT 
GTTGGGAATC CAGGTGAGAA AGGAAACAGA GGCAACCCAG GGTTACCAGG TCCGAAAGGC 
CTCGAGGGAT TGCCTGGGCT ACCAGGTCCT CCAGGCCCTA GAGGAGATAC GGGAAGCAGA 
GGAAATCCTG GAAGACCAGG ACCACACGGC ATGCCAGGAA GCATGGGGAT CATGGGGGTG 
CCAGGCCCTA AAGGGAGAAA AGGAACTTCA GGGCTTCCAG GTCTAGCTGG AAGACCAGGC 
CTGACAGGGA TCCATGGTCC CCAAGGAGAT AAGGGGGAGC CGGGTTATTC AGAAGGTGCA 
AGACCAGGAC CACCAGGACC AAAGGGCGAT CCAGGATTGC CAGGTGACAA AGGAAAGAAA 
GGAGAAAGAG GCGTACCTGG GCCACCTGGA CAATCGGGGC CTGCCGGACC TGATGGAGCC 
CCTGGGAGTC CTGGGAGTCC TGGTCACCCA GGAAAACCAG GTCCTGCTGG TGATTTGGGT 
CTGAAAGGAC AGAAAGGCTT CCCAGGCCCT CCAGGAAGCA CTGGCCCTCC AGGCCCTCCA 
GGACTCCCAG GACTTCCTGG GCCAATGGGT ATGAGAGGTG ACCAAGGACG TGATGGAATT 
CCTGGTCCTC CAGGAGAAAA AGGAGAAACA GGCTTGCTGG GGGCCTACCC AGGCCCAAAA 
GGGAGCCCTG GTGTACCAGG TGCCAAAGGA GACAGGGGAG TTCCAGGCTT GTCTGGCCTT 
CCCGGCAGGA AGGGGGTTAT GGGGGATGTT GGACCCCAAG GACCCCCAGG CACTGCTGGA 
CTCCCAGGGC CACCAGGTCT ACCTGGGGCA ATTATCCCTG GCCCCAAAGG AGACAGAGGT 
CTTCCTGGCT TAAGAGGAAA TCCAGGTGAG CCAGGTCCCC CTGGACCTCC AGGACCTATC 
GGAAAAGGCA TAAAAGGTGA CAAAGGATTT ATGGGCCCAC CTGGCCCCAA AGGCCTGCCT 
GGAACTGTAG GGGACATGGG CCCACCAGGT TTCCCGGGAG CACCAGGTAC CCCCGGTCTT 
CCCGGTGTCA GAGGTGATCC AGGATTCCCT GGATTTCCAG GCATAAAAGG AGAAAAGGGT 
AACCCTGGAT TTCTTGGGCC AATTGGACAT CCAGGACCAG TTGGGCCCAA AGGACCACCA 
GGTCCACGTG GAAAACCTGG CACTCTTAAG GTCATCTCTC TTCCGGGAAG CCCAGGGCCA 
CCTGGTGTAC CTGGACAGCC AGGAATGAAA GGAGACCCTG GACCCCTGGG ACTGCCAGGA 
ATCCCAGGAC CCTGTGGGCC AAGAGGTAAA CCAGGCAAGG ATGGGAAACC AGGAACTCCA 
GGACCAGCTG GTACAAAGGG CAACAAGGGC TTGAAAGGAC AGCAAGGCCC ACCTGGTCTG 
GATGGATTGC CAGGCTTAAA GGGAAATCCT GGTGACAGGG GAACGCCGGC CACTGGTACA 
AGAATGCGAG GCTTCATCTT CACCCGACAC AGTCAAACCA CGGCCATTCC TTCATGCCCT 
GAAGGAACAC AGCCACTCTA TAGTGGGTTT TCTCTTCTTT TTGTACAAGG AAACAAACGT 
GCACATGGAC AAGACCTAGG TACTCTGGGC AGCTGCCTGC AGCGATTCAC CACAATGCCG 
TTCTTATTCT GTAACATCAA TAATGTATGT AACTTTGCAT CACGAAATGA TTATTCATAC 
TGGCTGTCAA CACCAGCTCT GATGCCAATG GACATGGCTC CAATTAGTGG CAGAGCTCTC 
GAACCCTATA TTAGCAGATG CACCGTCTGT GAAGGTCCAG CAATGGCCAT AGCTGTTCAC 
AGTCAAACTA CTGCTATCCC TCCGTGTCCC CAGGACTGGG TTTCTCTCTG GAAAGGTTTT 
TCTTTCATTA TGTTCACAAG TGCAGGCTCT GAGGGTGCTG GACAAGCACT TGCCTCGCCT 
GGCTCCTGCC TGGAAGAATT CCGAGCCAGT CCATTTATAG AATGCCATGG ACGAGGGACA 
TGTAACTACT ACTCAAACTC CTACAGTTTC TGGCTGGCTT CGCTGAACCC AGAAAGAATG 
TTCAGAAAAC CTATTCCATC AACTGTGAAA GCTGGAGACT TAGAGAAAAT CATAAGCCGC 
TGTCAGGTGT GCATGAAGAA AAGACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pathogenicity of a Human Laminin
Journal of the American Society of Nephrology : JASN(2017 Dec)
Funk SD,Bayer RH,Malone AF,McKee KK,Yurchenco PD,Miner JH

Ultrastructural Characterization of the Glomerulopathy in Alport Mice by Helium Ion Scanning Microscopy (HIM).
Scientific reports7(1)11696(2017 Sep)
Tsuji K,Suleiman H,Miner JH,Daley JM,Capen DE,P?unescu TG,Lu HAJ

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Col4a3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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