Col3a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col3a1
Entrez Gene ID	12825
Full Name	collagen, type III, alpha 1
Synonyms	AW550625,Col3a-1,Tsk-2,Tsk2
General protein information	Preferred Names collagen, type III, alpha 1 Names collagen alpha-1(III) chain procollagen, type III, alpha 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C1.1\|1 23.67 cM
Summary	This gene encodes the alpha-1 subunit of the fibril-forming type III collagen found in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. The encoded protein forms homotrimeric type III procollagen that undergoes proteolytic processing during fibril formation. A majority of mice lacking the encoded protein die within two days of birth but about 5% of the animals survive to adulthood. The surviving mice exhibit severe cortical malformation and experience significantly shorter lifespan. The mutant mouse named 'tight skin 2' exhibiting systemic sclerosis phenotype was found to harbor a missense point mutation in this gene. A pseudogene of this gene has been defined on chromosome 8. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009930.2	NP_034060.2	collagen alpha-1(III) chain preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col3a1	NP_114474.1
	COL3A1	XP_001105968.2
Mus musculus (house mouse)	Col3a1	NP_034060.2
Homo sapiens (human)	COL3A1	NP_000081.1
Pan troglodytes (chimpanzee)	COL3A1	XP_001163809.1
Gallus gallus (chicken)	COL3A1	NP_990711.2
Xenopus tropicalis (tropical clawed frog)	col3a1	NP_001136379.1

Gene Ontology

The following Col3a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col3a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22549	NM_009930.2 Latest version!	Mus musculus collagen, type III, alpha 1 (Col3a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$860.30 $1229.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col3a1 ( NM_009930.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034060.2 Mus musculus collagen, type III, alpha 1 (Col3a1), mRNA.

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CloneID	OMu22549
Clone ID Related Accession (Same CDS sequence)	NM_009930.2
Accession Version	NM_009930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(III) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY139268.1, BC043089.1 and AK019448.2. On Apr 7, 2009 this sequence version replaced NM_009930.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK220558.1, AK159920.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGAGCT TTGTGCAAAG TGGAACCTGG TTTCTTCTCA CCCTTCTTCA TCCCACTCTT 
ATTTTGGCAC AGCAGTCCAA CGTAGATGAA TTGGGATGCA GCCACCTTGG TCAGTCCTAT 
GAGTCTAGAG ATGTCTGGAA GCCAGAACCA TGTCAAATAT GTGTCTGTGA CTCAGGATCT 
GTCCTTTGCG ATGACATAAT CTGTGATGAG GAGCCACTAG ACTGCCCCAA CCCAGAGATC 
CCATTTGGAG AATGTTGTGC AATATGCCCA CAGCCTTCTA CACCTGCTCC TGTGCTTCCT 
GATGGCCATG GACCTCAAGG CCCCAAGGGA GATCCGGGTC CTCCTGGCAT TCCTGGGAGA 
AATGGTGACC CTGGCCTTCC AGGACAACCA GGTCTCCCTG GGCCTCCTGG CTCCCCTGGA 
ATCTGTGAAT CATGTCCAAC TGGTGGCCAA AATTATTCTC CCCAATTCGA CTCATATGAT 
GTCAAGTCTG GAGTGGGAGG AATGGGTGGC TATCCGGGAC CAGCTGGTCC ACCAGGCCCT 
CCAGGACCCC CTGGTTCTTC TGGACATCCT GGATCTCCTG GTTCTCCTGG ATACCAAGGT 
CCCCCTGGTG AACCTGGTCA AGCTGGTCCG GCAGGCCCTC CAGGACCTCC TGGTGCTCTT 
GGTCCAGCTG GTCCTGCTGG AAAGGATGGA GAGTCAGGAA GACCTGGACG ACCTGGAGAG 
CGTGGACTGC CTGGACCTCC AGGTATCAAA GGCCCAGCTG GCATGCCTGG ATTCCCTGGT 
ATGAAAGGAC ACAGAGGCTT TGATGGACGC AATGGAGAAA AGGGAGAAAC AGGTGCTCCT 
GGACTGAAGG GTGAAAATGG TCTTCCAGGA GACAACGGAG CTCCTGGCCC CATGGGTCCT 
AGAGGGGCTC CTGGTGAGCG AGGACGACCA GGCCTTCCTG GAGCTGCAGG TGCTCGAGGC 
AATGATGGTG CTCGGGGCAG TGATGGGCAA CCTGGTCCCC CTGGCCCTCC TGGAACTGCA 
GGATTCCCTG GATCCCCTGG TGCTAAGGGT GAAGTTGGAC CTGCAGGGTC CCCTGGCTCA 
AATGGCTCAC CAGGACAAAG AGGGGAGCCT GGACCGCAGG GACATGCTGG TGCTCAAGGG 
CCTCCTGGCC CTCCCGGGAA TAACGGCAGT CCTGGTGGAA AGGGTGAAAT GGGTCCAGCT 
GGCATTCCTG GAGCCCCTGG ACTAATAGGA GCAAGGGGCC CCCCTGGACC AGCAGGAACT 
AATGGTATAC CCGGAACACG AGGTCCTTCA GGTGAACCCG GCAAGAACGG TGCGAAGGGA 
GAGCCAGGAG CACGCGGTGA ACGGGGCGAA GCTGGTTCCC CAGGAATTCC AGGACCTAAG 
GGCGAAGATG GCAAAGATGG ATCACCTGGA GAACCTGGTG CAAATGGGCT TCCAGGAGCC 
GCAGGAGAAA GGGGTCCTAG TGGCTTCCGA GGACCTGCAG GGCCAAATGG CATCCCAGGA 
GAAAAGGGTC CTCCCGGAGA GCGTGGTGGC CCAGGTCCTG CAGGACCCAG AGGAGTAGCT 
GGAGAACCTG GCCGAGATGG AACCCCTGGA GGTCCAGGGA TACGGGGTAT GCCCGGAAGT 
CCAGGTGGAC CAGGCAATGA TGGAAAACCA GGACCTCCTG GAAGTCAAGG AGAAAGTGGT 
CGCCCTGGTC CCCCTGGCCC ATCTGGTCCC AGAGGTCAGC CTGGTGTCAT GGGTTTCCCT 
GGTCCTAAAG GAAATGATGG TGCCCCTGGC AAGAATGGAG AACGGGGTGG CCCTGGAGGA 
CCTGGCCTTC CAGGTCCTGC TGGAAAGAAT GGGGAGACTG GACCTCAGGG TCCCCCAGGA 
CCTACTGGCC CAGCTGGTGA CAAGGGAGAT TCTGGACCCC CTGGTCCACA AGGATTACAA 
GGCATACCTG GTACCGGTGG TCCTCCAGGA GAAAATGGGA AACCAGGTGA ACCAGGTCCA 
AAGGGTGAAG TCGGTGCTCC TGGAGCTCCT GGAGGCAAGG GTGATTCTGG TGCACCTGGA 
GAACGTGGGC CTCCTGGAAC AGCAGGAATC CCTGGTGCTA GAGGTGGAGC TGGACCCCCT 
GGCCCTGAGG GAGGAAAGGG CCCTGCTGGT CCCCCTGGCC CTCCTGGTGC TTCTGGTTCT 
CCTGGTCTGC AAGGGATGCC AGGGGAAAGA GGAGGTCCTG GAAGTCCTGG CCCAAAGGGT 
GAAAAGGGTG AACCAGGTGG TGCAGGTGCT GACGGAGTTC CAGGAAAGGA TGGGCCAAGG 
GGTCCTGCTG GCCCTATTGG TCCCCCTGGC CCAGCTGGTC AGCCTGGAGA TAAGGGTGAA 
GGTGGTTCCC CTGGACTTCC AGGTATAGCT GGACCTCGAG GTGGCCCTGG TGAGAGAGGT 
GAACATGGCC CTCCAGGACC TGCTGGCTTC CCGGGTGCTC CTGGACAGAA TGGTGAACCA 
GGTGCTAAAG GAGAAAGAGG TGCCCCTGGA GAGAAAGGTG AAGGAGGCCC TCCAGGACCT 
GCAGGACCCA CTGGCAGTTC TGGACCTGCT GGTCCGCCTG GTCCTCAGGG TGTAAAGGGT 
GAACGTGGTA GTCCTGGTGG TCCTGGTACT GCTGGCTTTC CCGGTGGGCG TGGTCTTCCT 
GGTCCTCCTG GCAATAATGG TAATCCAGGG CCCCCAGGAC CCAGTGGTGC TCCTGGCAAG 
GACGGCCCTC CAGGTCCTGC AGGCAACAGT GGTTCTCCTG GCAACCCTGG AATAGCTGGA 
CCAAAAGGTG ATGCTGGACA GCCTGGAGAG AAGGGGCCAC CTGGTGCTCA GGGTCCTCCG 
GGTTCCCCAG GCCCACTTGG AATTGCAGGG CTAACTGGAG CACGAGGTCT TGCTGGACCA 
CCAGGCATGC CAGGTCCACG GGGTAGCCCT GGACCTCAGG GTATCAAGGG TGAAAGTGGA 
AAACCAGGAG CCAGTGGCCA TAATGGGGAA CGTGGTCCTC CTGGACCCCA AGGTCTTCCT 
GGTCAGCCTG GTACAGCTGG TGAACCTGGA AGGGATGGAA ACCCTGGATC AGATGGTCAG 
CCAGGTCGAG ATGGATCTCC TGGTGGCAAG GGTGATCGTG GTGAAAATGG TTCTCCTGGT 
GCCCCAGGCG CTCCTGGTCA TCCAGGCCCA CCTGGCCCTG TTGGTCCATC TGGAAAGAGT 
GGTGACAGAG GAGAAACTGG CCCTGCTGGT CCTTCTGGTG CTCCAGGCCC TGCTGGAGCT 
CGAGGTGCTC CGGGTCCCCA AGGTCCACGA GGTGACAAAG GTGAAACTGG TGAACGTGGC 
TCTAATGGCA TCAAAGGACA TCGAGGATTC CCTGGCAATC CAGGTCCTCC AGGTTCTCCT 
GGTGCTGCTG GTCACCAGGG TGCAATTGGT AGTCCAGGAC CTGCAGGTCC CAGAGGACCA 
GTTGGACCAC ATGGACCTCC TGGAAAAGAT GGAACAAGTG GGCATCCAGG TCCTATTGGA 
CCACCAGGTC CTAGAGGAAA CAGAGGTGAA AGAGGATCTG AGGGCTCGCC AGGCCACCCT 
GGACAGCCAG GACCCCCTGG ACCCCCTGGT GCCCCTGGTC CCTGCTGTGG TGGTGGTGCT 
GCTGCCATTG CTGGAGTTGG AGGTGAAAAG TCTGGTGGCT TTTCACCCTA TTATGGAGAC 
GATCCAATGG ATTTCAAGAT CAACACTGAA GAGATTATGT CTTCACTCAA GTCTGTTAAT 
GGACAAATAG AGAGTCTTAT AAGCCCTGAT GGTTCTCGAA AAAACCCTGC TCGGAACTGC 
AGAGACCTAA AATTCTGCCA CCCCGAACTC AAGAGTGGAG AATACTGGGT TGATCCTAAC 
CAAGGCTGCA AGATGGATGC TATAAAAGTA TTCTGTAACA TGGAAACTGG GGAAACATGC 
ATAAATGCCA GCCCCATGAC TGTCCCACGT AAGCACTGGT GGACAGATTC TGGTGCAGAG 
AAGAAACATG TTTGGTTTGG AGAATCTATG AATGGTGGTT TTCAGTTCAG CTATGGCCCT 
CCTGATCTTC CTGAAGATGT CGTTGATGTG CAGCTGGCAT TCCTCAGACT TCTTTCCAGC 
CGGGCCTCCC AGAACATTAC ATACCACTGC AAAAACAGTA TTGCCTATAT GGATCAGGCC 
AGTGGCAATG TAAAGAAGTC TCTGAAGCTG ATGGGATCAA ATGAAGGCGA ATTCAAGGCT 
GAAGGAAACA GCAAATTCAC TTACACAGTT CTAGAGGATG GCTGTACTAA ACACACTGGG 
GAATGGAGCA AGACAGTCTT TGAATATCAA ACACGCAAGG CAATGAGACT ACCCATCATA 
GATATCGCAC CCTATGACAT TGGGGGTCCT GATCAAGAAT TTGGTGTGGA CATTGGCCCT 
GTTTGCTTTT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034060.2
CDS	211..4605
Translation	MMSFVQSGTWFLLTLLHPTLILAQQSNVDELGCSHLGQSYESRDVWKPEPCQICVCDSGSVLCDDIICDEEPLDCPNPEIPFGECCAICPQPSTPAPVLPDGHGPQGPKGDPGPPGIPGRNGDPGLPGQPGLPGPPGSPGICESCPTGGQNYSPQFDSYDVKSGVGGMGGYPGPAGPPGPPGPPGSSGHPGSPGSPGYQGPPGEPGQAGPAGPPGPPGALGPAGPAGKDGESGRPGRPGERGLPGPPGIKGPAGMPGFPGMKGHRGFDGRNGEKGETGAPGLKGENGLPGDNGAPGPMGPRGAPGERGRPGLPGAAGARGNDGARGSDGQPGPPGPPGTAGFPGSPGAKGEVGPAGSPGSNGSPGQRGEPGPQGHAGAQGPPGPPGNNGSPGGKGEMGPAGIPGAPGLIGARGPPGPAGTNGIPGTRGPSGEPGKNGAKGEPGARGERGEAGSPGIPGPKGEDGKDGSPGEPGANGLPGAAGERGPSGFRGPAGPNGIPGEKGPPGERGGPGPAGPRGVAGEPGRDGTPGGPGIRGMPGSPGGPGNDGKPGPPGSQGESGRPGPPGPSGPRGQPGVMGFPGPKGNDGAPGKNGERGGPGGPGLPGPAGKNGETGPQGPPGPTGPAGDKGDSGPPGPQGLQGIPGTGGPPGENGKPGEPGPKGEVGAPGAPGGKGDSGAPGERGPPGTAGIPGARGGAGPPGPEGGKGPAGPPGPPGASGSPGLQGMPGERGGPGSPGPKGEKGEPGGAGADGVPGKDGPRGPAGPIGPPGPAGQPGDKGEGGSPGLPGIAGPRGGPGERGEHGPPGPAGFPGAPGQNGEPGAKGERGAPGEKGEGGPPGPAGPTGSSGPAGPPGPQGVKGERGSPGGPGTAGFPGGRGLPGPPGNNGNPGPPGPSGAPGKDGPPGPAGNSGSPGNPGIAGPKGDAGQPGEKGPPGAQGPPGSPGPLGIAGLTGARGLAGPPGMPGPRGSPGPQGIKGESGKPGASGHNGERGPPGPQGLPGQPGTAGEPGRDGNPGSDGQPGRDGSPGGKGDRGENGSPGAPGAPGHPGPPGPVGPSGKSGDRGETGPAGPSGAPGPAGARGAPGPQGPRGDKGETGERGSNGIKGHRGFPGNPGPPGSPGAAGHQGAIGSPGPAGPRGPVGPHGPPGKDGTSGHPGPIGPPGPRGNRGERGSEGSPGHPGQPGPPGPPGAPGPCCGGGAAAIAGVGGEKSGGFSPYYGDDPMDFKINTEEIMSSLKSVNGQIESLISPDGSRKNPARNCRDLKFCHPELKSGEYWVDPNQGCKMDAIKVFCNMETGETCINASPMTVPRKHWWTDSGAEKKHVWFGESMNGGFQFSYGPPDLPEDVVDVQLAFLRLLSSRASQNITYHCKNSIAYMDQASGNVKKSLKLMGSNEGEFKAEGNSKFTYTVLEDGCTKHTGEWSKTVFEYQTRKAMRLPIIDIAPYDIGGPDQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	NM_009930.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type III, alpha 1 (Col3a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009930.2

ORF Insert Sequence:

ATGATGAGCT TTGTGCAAAG TGGAACCTGG TTTCTTCTCA CCCTTCTTCA TCCCACTCTT 
ATTTTGGCAC AGCAGTCCAA CGTAGATGAA TTGGGATGCA GCCACCTTGG TCAGTCCTAT 
GAGTCTAGAG ATGTCTGGAA GCCAGAACCA TGTCAAATAT GTGTCTGTGA CTCAGGATCT 
GTCCTTTGCG ATGACATAAT CTGTGATGAG GAGCCACTAG ACTGCCCCAA CCCAGAGATC 
CCATTTGGAG AATGTTGTGC AATATGCCCA CAGCCTTCTA CACCTGCTCC TGTGCTTCCT 
GATGGCCATG GACCTCAAGG CCCCAAGGGA GATCCGGGTC CTCCTGGCAT TCCTGGGAGA 
AATGGTGACC CTGGCCTTCC AGGACAACCA GGTCTCCCTG GGCCTCCTGG CTCCCCTGGA 
ATCTGTGAAT CATGTCCAAC TGGTGGCCAA AATTATTCTC CCCAATTCGA CTCATATGAT 
GTCAAGTCTG GAGTGGGAGG AATGGGTGGC TATCCGGGAC CAGCTGGTCC ACCAGGCCCT 
CCAGGACCCC CTGGTTCTTC TGGACATCCT GGATCTCCTG GTTCTCCTGG ATACCAAGGT 
CCCCCTGGTG AACCTGGTCA AGCTGGTCCG GCAGGCCCTC CAGGACCTCC TGGTGCTCTT 
GGTCCAGCTG GTCCTGCTGG AAAGGATGGA GAGTCAGGAA GACCTGGACG ACCTGGAGAG 
CGTGGACTGC CTGGACCTCC AGGTATCAAA GGCCCAGCTG GCATGCCTGG ATTCCCTGGT 
ATGAAAGGAC ACAGAGGCTT TGATGGACGC AATGGAGAAA AGGGAGAAAC AGGTGCTCCT 
GGACTGAAGG GTGAAAATGG TCTTCCAGGA GACAACGGAG CTCCTGGCCC CATGGGTCCT 
AGAGGGGCTC CTGGTGAGCG AGGACGACCA GGCCTTCCTG GAGCTGCAGG TGCTCGAGGC 
AATGATGGTG CTCGGGGCAG TGATGGGCAA CCTGGTCCCC CTGGCCCTCC TGGAACTGCA 
GGATTCCCTG GATCCCCTGG TGCTAAGGGT GAAGTTGGAC CTGCAGGGTC CCCTGGCTCA 
AATGGCTCAC CAGGACAAAG AGGGGAGCCT GGACCGCAGG GACATGCTGG TGCTCAAGGG 
CCTCCTGGCC CTCCCGGGAA TAACGGCAGT CCTGGTGGAA AGGGTGAAAT GGGTCCAGCT 
GGCATTCCTG GAGCCCCTGG ACTAATAGGA GCAAGGGGCC CCCCTGGACC AGCAGGAACT 
AATGGTATAC CCGGAACACG AGGTCCTTCA GGTGAACCCG GCAAGAACGG TGCGAAGGGA 
GAGCCAGGAG CACGCGGTGA ACGGGGCGAA GCTGGTTCCC CAGGAATTCC AGGACCTAAG 
GGCGAAGATG GCAAAGATGG ATCACCTGGA GAACCTGGTG CAAATGGGCT TCCAGGAGCC 
GCAGGAGAAA GGGGTCCTAG TGGCTTCCGA GGACCTGCAG GGCCAAATGG CATCCCAGGA 
GAAAAGGGTC CTCCCGGAGA GCGTGGTGGC CCAGGTCCTG CAGGACCCAG AGGAGTAGCT 
GGAGAACCTG GCCGAGATGG AACCCCTGGA GGTCCAGGGA TACGGGGTAT GCCCGGAAGT 
CCAGGTGGAC CAGGCAATGA TGGAAAACCA GGACCTCCTG GAAGTCAAGG AGAAAGTGGT 
CGCCCTGGTC CCCCTGGCCC ATCTGGTCCC AGAGGTCAGC CTGGTGTCAT GGGTTTCCCT 
GGTCCTAAAG GAAATGATGG TGCCCCTGGC AAGAATGGAG AACGGGGTGG CCCTGGAGGA 
CCTGGCCTTC CAGGTCCTGC TGGAAAGAAT GGGGAGACTG GACCTCAGGG TCCCCCAGGA 
CCTACTGGCC CAGCTGGTGA CAAGGGAGAT TCTGGACCCC CTGGTCCACA AGGATTACAA 
GGCATACCTG GTACCGGTGG TCCTCCAGGA GAAAATGGGA AACCAGGTGA ACCAGGTCCA 
AAGGGTGAAG TCGGTGCTCC TGGAGCTCCT GGAGGCAAGG GTGATTCTGG TGCACCTGGA 
GAACGTGGGC CTCCTGGAAC AGCAGGAATC CCTGGTGCTA GAGGTGGAGC TGGACCCCCT 
GGCCCTGAGG GAGGAAAGGG CCCTGCTGGT CCCCCTGGCC CTCCTGGTGC TTCTGGTTCT 
CCTGGTCTGC AAGGGATGCC AGGGGAAAGA GGAGGTCCTG GAAGTCCTGG CCCAAAGGGT 
GAAAAGGGTG AACCAGGTGG TGCAGGTGCT GACGGAGTTC CAGGAAAGGA TGGGCCAAGG 
GGTCCTGCTG GCCCTATTGG TCCCCCTGGC CCAGCTGGTC AGCCTGGAGA TAAGGGTGAA 
GGTGGTTCCC CTGGACTTCC AGGTATAGCT GGACCTCGAG GTGGCCCTGG TGAGAGAGGT 
GAACATGGCC CTCCAGGACC TGCTGGCTTC CCGGGTGCTC CTGGACAGAA TGGTGAACCA 
GGTGCTAAAG GAGAAAGAGG TGCCCCTGGA GAGAAAGGTG AAGGAGGCCC TCCAGGACCT 
GCAGGACCCA CTGGCAGTTC TGGACCTGCT GGTCCGCCTG GTCCTCAGGG TGTAAAGGGT 
GAACGTGGTA GTCCTGGTGG TCCTGGTACT GCTGGCTTTC CCGGTGGGCG TGGTCTTCCT 
GGTCCTCCTG GCAATAATGG TAATCCAGGG CCCCCAGGAC CCAGTGGTGC TCCTGGCAAG 
GACGGCCCTC CAGGTCCTGC AGGCAACAGT GGTTCTCCTG GCAACCCTGG AATAGCTGGA 
CCAAAAGGTG ATGCTGGACA GCCTGGAGAG AAGGGGCCAC CTGGTGCTCA GGGTCCTCCG 
GGTTCCCCAG GCCCACTTGG AATTGCAGGG CTAACTGGAG CACGAGGTCT TGCTGGACCA 
CCAGGCATGC CAGGTCCACG GGGTAGCCCT GGACCTCAGG GTATCAAGGG TGAAAGTGGA 
AAACCAGGAG CCAGTGGCCA TAATGGGGAA CGTGGTCCTC CTGGACCCCA AGGTCTTCCT 
GGTCAGCCTG GTACAGCTGG TGAACCTGGA AGGGATGGAA ACCCTGGATC AGATGGTCAG 
CCAGGTCGAG ATGGATCTCC TGGTGGCAAG GGTGATCGTG GTGAAAATGG TTCTCCTGGT 
GCCCCAGGCG CTCCTGGTCA TCCAGGCCCA CCTGGCCCTG TTGGTCCATC TGGAAAGAGT 
GGTGACAGAG GAGAAACTGG CCCTGCTGGT CCTTCTGGTG CTCCAGGCCC TGCTGGAGCT 
CGAGGTGCTC CGGGTCCCCA AGGTCCACGA GGTGACAAAG GTGAAACTGG TGAACGTGGC 
TCTAATGGCA TCAAAGGACA TCGAGGATTC CCTGGCAATC CAGGTCCTCC AGGTTCTCCT 
GGTGCTGCTG GTCACCAGGG TGCAATTGGT AGTCCAGGAC CTGCAGGTCC CAGAGGACCA 
GTTGGACCAC ATGGACCTCC TGGAAAAGAT GGAACAAGTG GGCATCCAGG TCCTATTGGA 
CCACCAGGTC CTAGAGGAAA CAGAGGTGAA AGAGGATCTG AGGGCTCGCC AGGCCACCCT 
GGACAGCCAG GACCCCCTGG ACCCCCTGGT GCCCCTGGTC CCTGCTGTGG TGGTGGTGCT 
GCTGCCATTG CTGGAGTTGG AGGTGAAAAG TCTGGTGGCT TTTCACCCTA TTATGGAGAC 
GATCCAATGG ATTTCAAGAT CAACACTGAA GAGATTATGT CTTCACTCAA GTCTGTTAAT 
GGACAAATAG AGAGTCTTAT AAGCCCTGAT GGTTCTCGAA AAAACCCTGC TCGGAACTGC 
AGAGACCTAA AATTCTGCCA CCCCGAACTC AAGAGTGGAG AATACTGGGT TGATCCTAAC 
CAAGGCTGCA AGATGGATGC TATAAAAGTA TTCTGTAACA TGGAAACTGG GGAAACATGC 
ATAAATGCCA GCCCCATGAC TGTCCCACGT AAGCACTGGT GGACAGATTC TGGTGCAGAG 
AAGAAACATG TTTGGTTTGG AGAATCTATG AATGGTGGTT TTCAGTTCAG CTATGGCCCT 
CCTGATCTTC CTGAAGATGT CGTTGATGTG CAGCTGGCAT TCCTCAGACT TCTTTCCAGC 
CGGGCCTCCC AGAACATTAC ATACCACTGC AAAAACAGTA TTGCCTATAT GGATCAGGCC 
AGTGGCAATG TAAAGAAGTC TCTGAAGCTG ATGGGATCAA ATGAAGGCGA ATTCAAGGCT 
GAAGGAAACA GCAAATTCAC TTACACAGTT CTAGAGGATG GCTGTACTAA ACACACTGGG 
GAATGGAGCA AGACAGTCTT TGAATATCAA ACACGCAAGG CAATGAGACT ACCCATCATA 
GATATCGCAC CCTATGACAT TGGGGGTCCT GATCAAGAAT TTGGTGTGGA CATTGGCCCT 
GTTTGCTTTT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Col3a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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