Col2a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col2a1
Entrez Gene ID	12824
Full Name	collagen, type II, alpha 1
Synonyms	Col2,Col2a,Col2a-1,Del1,Dmm,Lpk,Rgsc856
General protein information	Preferred Names collagen, type II, alpha 1 Names collagen alpha-1(II) chain alpha-1 type II collagen chondrocalcin procollagen, type II, alpha 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 F1\|15 53.97 cM
Summary	This gene encodes the alpha-1 subunit of the fibril-forming type II collagen, the major component of cartilage and the vitreous humor of the eye. The encoded preproprotein forms homotrimeric, triple helical procollagen that undergoes proteolytic processing during fibirl formation. Mice harboring certain mutations in this gene exhibit severe chondrodysplasia characterized by short limbs and trunch, craniofacial deformities and cleft palate. A complete lack of the encoded protein in mice results in postnatal lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Dec 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006520386.3	XP_006520449.1	collagen alpha-1(II) chain isoform X1
NM_001113515.2	NP_001106987.2	collagen alpha-1(II) chain isoform 2 precursor
NM_031163.3	NP_112440.2	collagen alpha-1(II) chain isoform 1 preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL2A1	NP_001001135.2
Gallus gallus (chicken)	COL2A1	NP_989757.1
Xenopus tropicalis (tropical clawed frog)	col2a1	NP_989220.1
	COL2A1	XP_001100739.1
Canis lupus familiaris (dog)	COL2A1	NP_001006952.1
Rattus norvegicus (Norway rat)	Col2a1	NP_037061.1
Danio rerio (zebrafish)	col2a1a	NP_571367.1
Pan troglodytes (chimpanzee)	COL2A1	XP_509026.2
Homo sapiens (human)	COL2A1	NP_001835.3
Mus musculus (house mouse)	Col2a1	NP_112440.2

Gene Ontology

The following Col2a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col2a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22552	NM_001113515.2 Latest version!	Mus musculus collagen, type II, alpha 1 (Col2a1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$832.30 $1189.00
OMu22600	NM_031163.3 Latest version!	Mus musculus collagen, type II, alpha 1 (Col2a1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu50908	XM_006520386.3 Latest version!	Mus musculus collagen, type II, alpha 1 (Col2a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col2a1 ( NM_001113515.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001106987.2 Mus musculus collagen, type II, alpha 1 (Col2a1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22552
Clone ID Related Accession (Same CDS sequence)	NM_001113515.2
Accession Version	NM_001113515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(II) chain isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC113444.9 and BC082331.1. On Mar 7, 2008 this sequence version replaced NM_001113515.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051383.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCCGCC TCGGGGCTCC CCAGTCGCTG GTGCTGCTGA CGCTGCTCAT CGCCGCGGTC 
CTACGGTGTC AGGGCCAGGA TGCCCGAAAA TTAGGGCCAA AGGGGCAGAA AGGAGAACCT 
GGAGATATCA GAGATATCAT AGGACCCAGA GGACCTCCTG GCCCTCAGGG ACCTGCAGGT 
GAACAAGGAC CCAGAGGTGA TCGTGGTGAC AAGGGAGAAA AGGGTGCGCC TGGACCCCGT 
GGCAGAGATG GAGAACCTGG TACCCCTGGA AATCCTGGCC CCGCTGGCCC TCCAGGTCCC 
CCTGGTCCCC CTGGCCTTAG TGCAGGAAAC TTCGCGGCTC AGATGGCTGG AGGGTATGAC 
GAGAAGGCTG GTGGTGCCCA GATGGGAGTC ATGCAAGGGC CCATGGGCCC CATGGGACCC 
CGTGGACCCC CAGGCCCTGC CGGTGCCCCC GGCCCTCAAG GATTTCAAGG CAATCCTGGT 
GAACCTGGCG AGCCTGGTGT CTCTGGTCCC ATGGGTCCCC GAGGTCCTCC TGGCCCTGCT 
GGAAAACCTG GTGACGACGG TGAAGCTGGG AAGCCCGGAA AGTCTGGGGA AAGAGGCCTC 
CCTGGCCCTC AGGGTGCTCG TGGATTCCCA GGAACCCCGG GTCTCCCCGG TGTCAAGGGT 
CACAGAGGTT ACCCAGGCCT CGACGGTGCT AAGGGGGAAG CTGGTGCTCC GGGTGTGAAG 
GGTGAGAGTG GTTCCCCTGG TGAGAACGGA TCCCCGGGCC CAATGGGTCC CCGTGGCCTG 
CCTGGTGAGA GAGGACGGAC TGGCCCTGCT GGTGCTGCTG GTGCTCGGGG TAACGATGGC 
CAGCCAGGCC CCGCTGGACC TCCGGGTCCT GTGGGTCCCG CAGGTGGTCC TGGCTTCCCT 
GGTGCTCCTG GTGCCAAGGG CGAAGCTGGT CCCACTGGTG CTCGCGGTCC TGAAGGTGCT 
CAAGGTTCTC GTGGCGAGCC TGGCAATCCT GGGTCCCCTG GGCCTGCAGG TGCTTCTGGT 
AACCCAGGGA CTGATGGTAT TCCTGGAGCC AAAGGATCCG CTGGTGCTCC TGGAATTGCT 
GGTGCCCCTG GCTTCCCTGG GCCCCGTGGC CCTCCCGGTC CTCAAGGTGC AACTGGTCCC 
CTTGGCCCCA AAGGTCAGGC GGGTGAACCT GGCATTGCTG GCTTTAAAGG TGATCAAGGC 
CCCAAGGGAG AGACTGGACC TGCTGGGCCC CAAGGAGCCC CTGGCCCCGC TGGTGAAGAA 
GGCAAACGAG GTGCTCGAGG AGAGCCGGGT GGTGCTGGAC CAATCGGACC CCCTGGAGAG 
AGAGGTGCTC CTGGCAACCG TGGATTCCCA GGTCAAGATG GTCTGGCAGG TCCCAAGGGT 
GCCCCTGGAG AGCGAGGGCC CAGTGGCTTG ACTGGTCCCA AGGGAGCCAA CGGTGACCCG 
GGTCGTCCTG GAGAACCTGG TCTTCCTGGA GCCAGGGGTC TTACCGGTCG CCCTGGTGAC 
GCTGGTCCTC AAGGCAAAGT TGGTCCTTCT GGAGCCCCTG GTGAAGACGG TCGCCCTGGA 
CCTCCTGGTC CTCAGGGAGC TCGTGGGCAG CCTGGCGTCA TGGGTTTCCC TGGCCCCAAA 
GGTGCCAACG GCGAGCCTGG CAAAGCTGGT GAGAAGGGTC TGGCTGGCGC TCCTGGTCTG 
AGAGGTCTTC CTGGAAAAGA CGGTGAGACG GGAGCCGCAG GACCCCCCGG CCCCAGTGGA 
CCTGCTGGTG AACGAGGCGA GCAGGGCGCT CCTGGACCAT CAGGGTTCCA GGGACTTCCT 
GGCCCTCCCG GTCCCCCAGG TGAAGGTGGA AAGCAAGGTG ACCAGGGTAT TCCTGGTGAA 
GCTGGAGCTC CTGGCCTTGT GGGTCCTCGG GGCGAGCGAG GTTTCCCAGG TGAACGTGGC 
TCTCCTGGTG CTCAGGGCCT TCAGGGTCCC CGAGGCCTCC CTGGCACTCC TGGTACTGAT 
GGTCCCAAAG GTGCAGCTGG CCCAGATGGC CCCCCTGGGG CTCAGGGGCC TCCAGGTCTA 
CAGGGAATGC CTGGTGAGAG AGGAGCCGCT GGCATTGCTG GGCCCAAGGG AGACAGAGGC 
GATGTTGGCG AGAAAGGCCC AGAGGGAGCT CCTGGGAAGG ATGGCGGCCG AGGTCTGACT 
GGGCCCATCG GACCCCCAGG CCCAGCAGGG GCCAACGGCG AGAAGGGAGA AGTCGGACCT 
CCTGGCCCGT CAGGAAGTAC CGGAGCTCGA GGTGCCCCGG GTGAACGCGG AGAGACCGGG 
CCACCTGGAC CTGCTGGATT CGCTGGCCCT CCTGGTGCTG ATGGCCAGCC TGGTGCCAAG 
GGTGATCAAG GAGAAGCCGG ACAGAAAGGA GATGCTGGTG CCCCCGGCCC ACAAGGCCCC 
TCGGGAGCCC CTGGGCCACA GGGTCCTACT GGAGTGACTG GTCCTAAGGG AGCCCGAGGT 
GCCCAAGGTC CCCCGGGAGC CACCGGATTC CCTGGAGCTG CTGGCCGAGT TGGACCCCCA 
GGTGCTAATG GCAATCCTGG ACCCGCCGGT CCCCCTGGTC CTGCTGGAAA AGATGGTCCC 
AAAGGTGTTC GAGGAGACAG TGGCCCCCCT GGCAGAGCTG GTGACCCCGG TCTTCAAGGT 
CCTGCAGGAG CTCCTGGCGA GAAAGGAGAA CCTGGAGATG ATGGTCCCTC TGGTCTTGAT 
GGTCCTCCAG GTCCCCAGGG GCTGGCTGGT CAAAGGGGCA TTGTTGGTCT GCCTGGTCAG 
CGTGGTGAGA GAGGATTCCC CGGCCTTCCC GGCCCATCGG GTGAGCCCGG CAAGCAGGGT 
GCACCTGGCG CGTCTGGAGA CAGAGGTCCT CCTGGTCCTG TGGGGCCTCC TGGCCTGACA 
GGGCCTGCAG GTGAACCTGG ACGAGAGGGC AGCCCTGGTG CTGATGGACC CCCTGGAAGA 
GATGGTGCAG CTGGAGTCAA GGGAGATCGT GGTGAGACTG GAGCACTGGG TGCCCCTGGA 
GCTCCTGGGC CCCCAGGCTC TCCTGGTCCT GCTGGCCCAA CTGGCAAACA AGGAGACAGA 
GGAGAGGCTG GTGCACAAGG TCCTATGGGT CCCTCAGGAC CTGCTGGAGC CCGTGGGATT 
GCAGGCCCTC AAGGCCCCCG AGGTGACAAA GGAGAATCTG GAGAGCAGGG CGAGAGGGGA 
CTGAAGGGAC ACCGAGGTTT CACTGGACTG CAGGGTCTGC CTGGCCCTCC GGGTCCTTCT 
GGAGATCAGG GTGCTTCTGG CCCTGCTGGT CCTTCTGGCC CTAGAGGTCC ACCTGGCCCT 
GTTGGTCCCT CTGGCAAAGA TGGCTCTAAT GGAATCCCTG GCCCCATCGG GCCTCCAGGT 
CCCCGTGGAC GCTCAGGAGA AACAGGCCCT GTTGGTCCCC CTGGAAGTCC CGGTCCTCCT 
GGCCCTCCAG GTCCTCCTGG TCCTGGCATC GACATGTCAG CCTTTGCTGG CTTAGGGCAG 
AGAGAGAAGG GCCCCGACCC CATGCAGTAC ATGCGGGCCG ACGAGGCAGA CAGTACCTTG 
AGACAGCACG ACGTGGAGGT GGACGCTACA CTCAAGTCAC TGAACAACCA GATTGAGAGC 
ATCCGCAGCC CCGACGGCTC CCGCAAGAAC CCTGCTCGCA CTTGCCAAGA CCTGAAACTC 
TGCCACCCCG AGTGGAAGAG CGGAGACTAC TGGATTGATC CCAACCAGGG CTGCACCTTG 
GACGCCATGA AAGTTTTCTG CAACATGGAG ACCGGCGAGA CTTGCGTCTA CCCCAACCCA 
GCGACTGTCC CTCGGAAAAA CTGGTGGAGC AGCAAGAGCA AGGAAAAGAA ACACATCTGG 
TTTGGAGAGA CCATGAACGG TGGCTTCCAC TTCAGCTATG GCGATGGCAA CCTGGCTCCC 
AACACCGCTA ACGTCCAGAT GACTTTCCTC CGTCTACTGT CCACTGAGGG CTCCCAGAAC 
ATCACCTACC ACTGTAAGAA CAGCATCGCC TACCTGGACG AAGCGGCTGG CAACCTCAAG 
AAGGCCTTGC TCATCCAGGG CTCCAATGAT GTAGAGATGA GGGCCGAGGG CAACAGCAGG 
TTCACATACA CTGCCCTGAA GGATGGCTGC ACGAAACACA CTGGTAAGTG GGGCAAGACC 
GTCATCGAGT ACCGATCACA GAAGACCTCC CGCCTTCCCA TTATTGACAT CGCACCCATG 
GACATTGGAG GGGCTGAACA GGAATTTGGT GTGGACATAG GGCCTGTCTG CTTCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106987.2
CDS	232..4491
Translation	MIRLGAPQSLVLLTLLIAAVLRCQGQDARKLGPKGQKGEPGDIRDIIGPRGPPGPQGPAGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPAGPPGPPGPPGLSAGNFAAQMAGGYDEKAGGAQMGVMQGPMGPMGPRGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGLPGPQGARGFPGTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDGQPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGSRGEPGNPGSPGPAGASGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQAGEPGIAGFKGDQGPKGETGPAGPQGAPGPAGEEGKRGARGEPGGAGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLTGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAGAPGLRGLPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGDQGIPGEAGAPGLVGPRGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPPGAQGPPGLQGMPGERGAAGIAGPKGDRGDVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGPPGADGQPGAKGDQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGANGNPGPAGPPGPAGKDGPKGVRGDSGPPGRAGDPGLQGPAGAPGEKGEPGDDGPSGLDGPPGPQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGRDGAAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIAGPQGPRGDKGESGEQGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPGPRGRSGETGPVGPPGSPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPMQYMRADEADSTLRQHDVEVDATLKSLNNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPATVPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGAEQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	NM_001113515.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type II, alpha 1 (Col2a1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113515.2

ORF Insert Sequence:

ATGATCCGCC TCGGGGCTCC CCAGTCGCTG GTGCTGCTGA CGCTGCTCAT CGCCGCGGTC 
CTACGGTGTC AGGGCCAGGA TGCCCGAAAA TTAGGGCCAA AGGGGCAGAA AGGAGAACCT 
GGAGATATCA GAGATATCAT AGGACCCAGA GGACCTCCTG GCCCTCAGGG ACCTGCAGGT 
GAACAAGGAC CCAGAGGTGA TCGTGGTGAC AAGGGAGAAA AGGGTGCGCC TGGACCCCGT 
GGCAGAGATG GAGAACCTGG TACCCCTGGA AATCCTGGCC CCGCTGGCCC TCCAGGTCCC 
CCTGGTCCCC CTGGCCTTAG TGCAGGAAAC TTCGCGGCTC AGATGGCTGG AGGGTATGAC 
GAGAAGGCTG GTGGTGCCCA GATGGGAGTC ATGCAAGGGC CCATGGGCCC CATGGGACCC 
CGTGGACCCC CAGGCCCTGC CGGTGCCCCC GGCCCTCAAG GATTTCAAGG CAATCCTGGT 
GAACCTGGCG AGCCTGGTGT CTCTGGTCCC ATGGGTCCCC GAGGTCCTCC TGGCCCTGCT 
GGAAAACCTG GTGACGACGG TGAAGCTGGG AAGCCCGGAA AGTCTGGGGA AAGAGGCCTC 
CCTGGCCCTC AGGGTGCTCG TGGATTCCCA GGAACCCCGG GTCTCCCCGG TGTCAAGGGT 
CACAGAGGTT ACCCAGGCCT CGACGGTGCT AAGGGGGAAG CTGGTGCTCC GGGTGTGAAG 
GGTGAGAGTG GTTCCCCTGG TGAGAACGGA TCCCCGGGCC CAATGGGTCC CCGTGGCCTG 
CCTGGTGAGA GAGGACGGAC TGGCCCTGCT GGTGCTGCTG GTGCTCGGGG TAACGATGGC 
CAGCCAGGCC CCGCTGGACC TCCGGGTCCT GTGGGTCCCG CAGGTGGTCC TGGCTTCCCT 
GGTGCTCCTG GTGCCAAGGG CGAAGCTGGT CCCACTGGTG CTCGCGGTCC TGAAGGTGCT 
CAAGGTTCTC GTGGCGAGCC TGGCAATCCT GGGTCCCCTG GGCCTGCAGG TGCTTCTGGT 
AACCCAGGGA CTGATGGTAT TCCTGGAGCC AAAGGATCCG CTGGTGCTCC TGGAATTGCT 
GGTGCCCCTG GCTTCCCTGG GCCCCGTGGC CCTCCCGGTC CTCAAGGTGC AACTGGTCCC 
CTTGGCCCCA AAGGTCAGGC GGGTGAACCT GGCATTGCTG GCTTTAAAGG TGATCAAGGC 
CCCAAGGGAG AGACTGGACC TGCTGGGCCC CAAGGAGCCC CTGGCCCCGC TGGTGAAGAA 
GGCAAACGAG GTGCTCGAGG AGAGCCGGGT GGTGCTGGAC CAATCGGACC CCCTGGAGAG 
AGAGGTGCTC CTGGCAACCG TGGATTCCCA GGTCAAGATG GTCTGGCAGG TCCCAAGGGT 
GCCCCTGGAG AGCGAGGGCC CAGTGGCTTG ACTGGTCCCA AGGGAGCCAA CGGTGACCCG 
GGTCGTCCTG GAGAACCTGG TCTTCCTGGA GCCAGGGGTC TTACCGGTCG CCCTGGTGAC 
GCTGGTCCTC AAGGCAAAGT TGGTCCTTCT GGAGCCCCTG GTGAAGACGG TCGCCCTGGA 
CCTCCTGGTC CTCAGGGAGC TCGTGGGCAG CCTGGCGTCA TGGGTTTCCC TGGCCCCAAA 
GGTGCCAACG GCGAGCCTGG CAAAGCTGGT GAGAAGGGTC TGGCTGGCGC TCCTGGTCTG 
AGAGGTCTTC CTGGAAAAGA CGGTGAGACG GGAGCCGCAG GACCCCCCGG CCCCAGTGGA 
CCTGCTGGTG AACGAGGCGA GCAGGGCGCT CCTGGACCAT CAGGGTTCCA GGGACTTCCT 
GGCCCTCCCG GTCCCCCAGG TGAAGGTGGA AAGCAAGGTG ACCAGGGTAT TCCTGGTGAA 
GCTGGAGCTC CTGGCCTTGT GGGTCCTCGG GGCGAGCGAG GTTTCCCAGG TGAACGTGGC 
TCTCCTGGTG CTCAGGGCCT TCAGGGTCCC CGAGGCCTCC CTGGCACTCC TGGTACTGAT 
GGTCCCAAAG GTGCAGCTGG CCCAGATGGC CCCCCTGGGG CTCAGGGGCC TCCAGGTCTA 
CAGGGAATGC CTGGTGAGAG AGGAGCCGCT GGCATTGCTG GGCCCAAGGG AGACAGAGGC 
GATGTTGGCG AGAAAGGCCC AGAGGGAGCT CCTGGGAAGG ATGGCGGCCG AGGTCTGACT 
GGGCCCATCG GACCCCCAGG CCCAGCAGGG GCCAACGGCG AGAAGGGAGA AGTCGGACCT 
CCTGGCCCGT CAGGAAGTAC CGGAGCTCGA GGTGCCCCGG GTGAACGCGG AGAGACCGGG 
CCACCTGGAC CTGCTGGATT CGCTGGCCCT CCTGGTGCTG ATGGCCAGCC TGGTGCCAAG 
GGTGATCAAG GAGAAGCCGG ACAGAAAGGA GATGCTGGTG CCCCCGGCCC ACAAGGCCCC 
TCGGGAGCCC CTGGGCCACA GGGTCCTACT GGAGTGACTG GTCCTAAGGG AGCCCGAGGT 
GCCCAAGGTC CCCCGGGAGC CACCGGATTC CCTGGAGCTG CTGGCCGAGT TGGACCCCCA 
GGTGCTAATG GCAATCCTGG ACCCGCCGGT CCCCCTGGTC CTGCTGGAAA AGATGGTCCC 
AAAGGTGTTC GAGGAGACAG TGGCCCCCCT GGCAGAGCTG GTGACCCCGG TCTTCAAGGT 
CCTGCAGGAG CTCCTGGCGA GAAAGGAGAA CCTGGAGATG ATGGTCCCTC TGGTCTTGAT 
GGTCCTCCAG GTCCCCAGGG GCTGGCTGGT CAAAGGGGCA TTGTTGGTCT GCCTGGTCAG 
CGTGGTGAGA GAGGATTCCC CGGCCTTCCC GGCCCATCGG GTGAGCCCGG CAAGCAGGGT 
GCACCTGGCG CGTCTGGAGA CAGAGGTCCT CCTGGTCCTG TGGGGCCTCC TGGCCTGACA 
GGGCCTGCAG GTGAACCTGG ACGAGAGGGC AGCCCTGGTG CTGATGGACC CCCTGGAAGA 
GATGGTGCAG CTGGAGTCAA GGGAGATCGT GGTGAGACTG GAGCACTGGG TGCCCCTGGA 
GCTCCTGGGC CCCCAGGCTC TCCTGGTCCT GCTGGCCCAA CTGGCAAACA AGGAGACAGA 
GGAGAGGCTG GTGCACAAGG TCCTATGGGT CCCTCAGGAC CTGCTGGAGC CCGTGGGATT 
GCAGGCCCTC AAGGCCCCCG AGGTGACAAA GGAGAATCTG GAGAGCAGGG CGAGAGGGGA 
CTGAAGGGAC ACCGAGGTTT CACTGGACTG CAGGGTCTGC CTGGCCCTCC GGGTCCTTCT 
GGAGATCAGG GTGCTTCTGG CCCTGCTGGT CCTTCTGGCC CTAGAGGTCC ACCTGGCCCT 
GTTGGTCCCT CTGGCAAAGA TGGCTCTAAT GGAATCCCTG GCCCCATCGG GCCTCCAGGT 
CCCCGTGGAC GCTCAGGAGA AACAGGCCCT GTTGGTCCCC CTGGAAGTCC CGGTCCTCCT 
GGCCCTCCAG GTCCTCCTGG TCCTGGCATC GACATGTCAG CCTTTGCTGG CTTAGGGCAG 
AGAGAGAAGG GCCCCGACCC CATGCAGTAC ATGCGGGCCG ACGAGGCAGA CAGTACCTTG 
AGACAGCACG ACGTGGAGGT GGACGCTACA CTCAAGTCAC TGAACAACCA GATTGAGAGC 
ATCCGCAGCC CCGACGGCTC CCGCAAGAAC CCTGCTCGCA CTTGCCAAGA CCTGAAACTC 
TGCCACCCCG AGTGGAAGAG CGGAGACTAC TGGATTGATC CCAACCAGGG CTGCACCTTG 
GACGCCATGA AAGTTTTCTG CAACATGGAG ACCGGCGAGA CTTGCGTCTA CCCCAACCCA 
GCGACTGTCC CTCGGAAAAA CTGGTGGAGC AGCAAGAGCA AGGAAAAGAA ACACATCTGG 
TTTGGAGAGA CCATGAACGG TGGCTTCCAC TTCAGCTATG GCGATGGCAA CCTGGCTCCC 
AACACCGCTA ACGTCCAGAT GACTTTCCTC CGTCTACTGT CCACTGAGGG CTCCCAGAAC 
ATCACCTACC ACTGTAAGAA CAGCATCGCC TACCTGGACG AAGCGGCTGG CAACCTCAAG 
AAGGCCTTGC TCATCCAGGG CTCCAATGAT GTAGAGATGA GGGCCGAGGG CAACAGCAGG 
TTCACATACA CTGCCCTGAA GGATGGCTGC ACGAAACACA CTGGTAAGTG GGGCAAGACC 
GTCATCGAGT ACCGATCACA GAAGACCTCC CGCCTTCCCA TTATTGACAT CGCACCCATG 
GACATTGGAG GGGCTGAACA GGAATTTGGT GTGGACATAG GGCCTGTCTG CTTCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col2a1 ( NM_031163.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_112440.2 Mus musculus collagen, type II, alpha 1 (Col2a1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22600
Clone ID Related Accession (Same CDS sequence)	NM_031163.3
Accession Version	NM_031163.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(II) chain isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC113444.9 and BC082331.1. On Jan 24, 2008 this sequence version replaced NM_031163.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC082331.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCCGCC TCGGGGCTCC CCAGTCGCTG GTGCTGCTGA CGCTGCTCAT CGCCGCGGTC 
CTACGGTGTC AGGGCCAGGA TGCCCAGGAG GCTGGCAGCT GTCTGCAGAA TGGGCAGAGG 
TATAAAGATA AGGATGTATG GAAGCCCTCA TCTTGCCGCA TCTGTGTGTG TGACACTGGG 
AATGTCCTCT GCGATGACAT TATCTGTGAA GACCCAGACT GCCTCAACCC CGAGATCCCC 
TTCGGAGAGT GCTGTCCCAT CTGCCCAGCT GACCTCGCCA CTGCCAGTGG AAAATTAGGG 
CCAAAGGGGC AGAAAGGAGA ACCTGGAGAT ATCAGAGATA TCATAGGACC CAGAGGACCT 
CCTGGCCCTC AGGGACCTGC AGGTGAACAA GGACCCAGAG GTGATCGTGG TGACAAGGGA 
GAAAAGGGTG CGCCTGGACC CCGTGGCAGA GATGGAGAAC CTGGTACCCC TGGAAATCCT 
GGCCCCGCTG GCCCTCCAGG TCCCCCTGGT CCCCCTGGCC TTAGTGCAGG AAACTTCGCG 
GCTCAGATGG CTGGAGGGTA TGACGAGAAG GCTGGTGGTG CCCAGATGGG AGTCATGCAA 
GGGCCCATGG GCCCCATGGG ACCCCGTGGA CCCCCAGGCC CTGCCGGTGC CCCCGGCCCT 
CAAGGATTTC AAGGCAATCC TGGTGAACCT GGCGAGCCTG GTGTCTCTGG TCCCATGGGT 
CCCCGAGGTC CTCCTGGCCC TGCTGGAAAA CCTGGTGACG ACGGTGAAGC TGGGAAGCCC 
GGAAAGTCTG GGGAAAGAGG CCTCCCTGGC CCTCAGGGTG CTCGTGGATT CCCAGGAACC 
CCGGGTCTCC CCGGTGTCAA GGGTCACAGA GGTTACCCAG GCCTCGACGG TGCTAAGGGG 
GAAGCTGGTG CTCCGGGTGT GAAGGGTGAG AGTGGTTCCC CTGGTGAGAA CGGATCCCCG 
GGCCCAATGG GTCCCCGTGG CCTGCCTGGT GAGAGAGGAC GGACTGGCCC TGCTGGTGCT 
GCTGGTGCTC GGGGTAACGA TGGCCAGCCA GGCCCCGCTG GACCTCCGGG TCCTGTGGGT 
CCCGCAGGTG GTCCTGGCTT CCCTGGTGCT CCTGGTGCCA AGGGCGAAGC TGGTCCCACT 
GGTGCTCGCG GTCCTGAAGG TGCTCAAGGT TCTCGTGGCG AGCCTGGCAA TCCTGGGTCC 
CCTGGGCCTG CAGGTGCTTC TGGTAACCCA GGGACTGATG GTATTCCTGG AGCCAAAGGA 
TCCGCTGGTG CTCCTGGAAT TGCTGGTGCC CCTGGCTTCC CTGGGCCCCG TGGCCCTCCC 
GGTCCTCAAG GTGCAACTGG TCCCCTTGGC CCCAAAGGTC AGGCGGGTGA ACCTGGCATT 
GCTGGCTTTA AAGGTGATCA AGGCCCCAAG GGAGAGACTG GACCTGCTGG GCCCCAAGGA 
GCCCCTGGCC CCGCTGGTGA AGAAGGCAAA CGAGGTGCTC GAGGAGAGCC GGGTGGTGCT 
GGACCAATCG GACCCCCTGG AGAGAGAGGT GCTCCTGGCA ACCGTGGATT CCCAGGTCAA 
GATGGTCTGG CAGGTCCCAA GGGTGCCCCT GGAGAGCGAG GGCCCAGTGG CTTGACTGGT 
CCCAAGGGAG CCAACGGTGA CCCGGGTCGT CCTGGAGAAC CTGGTCTTCC TGGAGCCAGG 
GGTCTTACCG GTCGCCCTGG TGACGCTGGT CCTCAAGGCA AAGTTGGTCC TTCTGGAGCC 
CCTGGTGAAG ACGGTCGCCC TGGACCTCCT GGTCCTCAGG GAGCTCGTGG GCAGCCTGGC 
GTCATGGGTT TCCCTGGCCC CAAAGGTGCC AACGGCGAGC CTGGCAAAGC TGGTGAGAAG 
GGTCTGGCTG GCGCTCCTGG TCTGAGAGGT CTTCCTGGAA AAGACGGTGA GACGGGAGCC 
GCAGGACCCC CCGGCCCCAG TGGACCTGCT GGTGAACGAG GCGAGCAGGG CGCTCCTGGA 
CCATCAGGGT TCCAGGGACT TCCTGGCCCT CCCGGTCCCC CAGGTGAAGG TGGAAAGCAA 
GGTGACCAGG GTATTCCTGG TGAAGCTGGA GCTCCTGGCC TTGTGGGTCC TCGGGGCGAG 
CGAGGTTTCC CAGGTGAACG TGGCTCTCCT GGTGCTCAGG GCCTTCAGGG TCCCCGAGGC 
CTCCCTGGCA CTCCTGGTAC TGATGGTCCC AAAGGTGCAG CTGGCCCAGA TGGCCCCCCT 
GGGGCTCAGG GGCCTCCAGG TCTACAGGGA ATGCCTGGTG AGAGAGGAGC CGCTGGCATT 
GCTGGGCCCA AGGGAGACAG AGGCGATGTT GGCGAGAAAG GCCCAGAGGG AGCTCCTGGG 
AAGGATGGCG GCCGAGGTCT GACTGGGCCC ATCGGACCCC CAGGCCCAGC AGGGGCCAAC 
GGCGAGAAGG GAGAAGTCGG ACCTCCTGGC CCGTCAGGAA GTACCGGAGC TCGAGGTGCC 
CCGGGTGAAC GCGGAGAGAC CGGGCCACCT GGACCTGCTG GATTCGCTGG CCCTCCTGGT 
GCTGATGGCC AGCCTGGTGC CAAGGGTGAT CAAGGAGAAG CCGGACAGAA AGGAGATGCT 
GGTGCCCCCG GCCCACAAGG CCCCTCGGGA GCCCCTGGGC CACAGGGTCC TACTGGAGTG 
ACTGGTCCTA AGGGAGCCCG AGGTGCCCAA GGTCCCCCGG GAGCCACCGG ATTCCCTGGA 
GCTGCTGGCC GAGTTGGACC CCCAGGTGCT AATGGCAATC CTGGACCCGC CGGTCCCCCT 
GGTCCTGCTG GAAAAGATGG TCCCAAAGGT GTTCGAGGAG ACAGTGGCCC CCCTGGCAGA 
GCTGGTGACC CCGGTCTTCA AGGTCCTGCA GGAGCTCCTG GCGAGAAAGG AGAACCTGGA 
GATGATGGTC CCTCTGGTCT TGATGGTCCT CCAGGTCCCC AGGGGCTGGC TGGTCAAAGG 
GGCATTGTTG GTCTGCCTGG TCAGCGTGGT GAGAGAGGAT TCCCCGGCCT TCCCGGCCCA 
TCGGGTGAGC CCGGCAAGCA GGGTGCACCT GGCGCGTCTG GAGACAGAGG TCCTCCTGGT 
CCTGTGGGGC CTCCTGGCCT GACAGGGCCT GCAGGTGAAC CTGGACGAGA GGGCAGCCCT 
GGTGCTGATG GACCCCCTGG AAGAGATGGT GCAGCTGGAG TCAAGGGAGA TCGTGGTGAG 
ACTGGAGCAC TGGGTGCCCC TGGAGCTCCT GGGCCCCCAG GCTCTCCTGG TCCTGCTGGC 
CCAACTGGCA AACAAGGAGA CAGAGGAGAG GCTGGTGCAC AAGGTCCTAT GGGTCCCTCA 
GGACCTGCTG GAGCCCGTGG GATTGCAGGC CCTCAAGGCC CCCGAGGTGA CAAAGGAGAA 
TCTGGAGAGC AGGGCGAGAG GGGACTGAAG GGACACCGAG GTTTCACTGG ACTGCAGGGT 
CTGCCTGGCC CTCCGGGTCC TTCTGGAGAT CAGGGTGCTT CTGGCCCTGC TGGTCCTTCT 
GGCCCTAGAG GTCCACCTGG CCCTGTTGGT CCCTCTGGCA AAGATGGCTC TAATGGAATC 
CCTGGCCCCA TCGGGCCTCC AGGTCCCCGT GGACGCTCAG GAGAAACAGG CCCTGTTGGT 
CCCCCTGGAA GTCCCGGTCC TCCTGGCCCT CCAGGTCCTC CTGGTCCTGG CATCGACATG 
TCAGCCTTTG CTGGCTTAGG GCAGAGAGAG AAGGGCCCCG ACCCCATGCA GTACATGCGG 
GCCGACGAGG CAGACAGTAC CTTGAGACAG CACGACGTGG AGGTGGACGC TACACTCAAG 
TCACTGAACA ACCAGATTGA GAGCATCCGC AGCCCCGACG GCTCCCGCAA GAACCCTGCT 
CGCACTTGCC AAGACCTGAA ACTCTGCCAC CCCGAGTGGA AGAGCGGAGA CTACTGGATT 
GATCCCAACC AGGGCTGCAC CTTGGACGCC ATGAAAGTTT TCTGCAACAT GGAGACCGGC 
GAGACTTGCG TCTACCCCAA CCCAGCGACT GTCCCTCGGA AAAACTGGTG GAGCAGCAAG 
AGCAAGGAAA AGAAACACAT CTGGTTTGGA GAGACCATGA ACGGTGGCTT CCACTTCAGC 
TATGGCGATG GCAACCTGGC TCCCAACACC GCTAACGTCC AGATGACTTT CCTCCGTCTA 
CTGTCCACTG AGGGCTCCCA GAACATCACC TACCACTGTA AGAACAGCAT CGCCTACCTG 
GACGAAGCGG CTGGCAACCT CAAGAAGGCC TTGCTCATCC AGGGCTCCAA TGATGTAGAG 
ATGAGGGCCG AGGGCAACAG CAGGTTCACA TACACTGCCC TGAAGGATGG CTGCACGAAA 
CACACTGGTA AGTGGGGCAA GACCGTCATC GAGTACCGAT CACAGAAGAC CTCCCGCCTT 
CCCATTATTG ACATCGCACC CATGGACATT GGAGGGGCTG AACAGGAATT TGGTGTGGAC 
ATAGGGCCTG TCTGCTTCTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112440.2
CDS	232..4695
Translation	MIRLGAPQSLVLLTLLIAAVLRCQGQDAQEAGSCLQNGQRYKDKDVWKPSSCRICVCDTGNVLCDDIICEDPDCLNPEIPFGECCPICPADLATASGKLGPKGQKGEPGDIRDIIGPRGPPGPQGPAGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPAGPPGPPGPPGLSAGNFAAQMAGGYDEKAGGAQMGVMQGPMGPMGPRGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGLPGPQGARGFPGTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDGQPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGSRGEPGNPGSPGPAGASGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQAGEPGIAGFKGDQGPKGETGPAGPQGAPGPAGEEGKRGARGEPGGAGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLTGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAGAPGLRGLPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGDQGIPGEAGAPGLVGPRGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPPGAQGPPGLQGMPGERGAAGIAGPKGDRGDVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGPPGADGQPGAKGDQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGANGNPGPAGPPGPAGKDGPKGVRGDSGPPGRAGDPGLQGPAGAPGEKGEPGDDGPSGLDGPPGPQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGRDGAAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIAGPQGPRGDKGESGEQGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPGPRGRSGETGPVGPPGSPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPMQYMRADEADSTLRQHDVEVDATLKSLNNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPATVPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGAEQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	NM_031163.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type II, alpha 1 (Col2a1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031163.3

ORF Insert Sequence:

ATGATCCGCC TCGGGGCTCC CCAGTCGCTG GTGCTGCTGA CGCTGCTCAT CGCCGCGGTC 
CTACGGTGTC AGGGCCAGGA TGCCCAGGAG GCTGGCAGCT GTCTGCAGAA TGGGCAGAGG 
TATAAAGATA AGGATGTATG GAAGCCCTCA TCTTGCCGCA TCTGTGTGTG TGACACTGGG 
AATGTCCTCT GCGATGACAT TATCTGTGAA GACCCAGACT GCCTCAACCC CGAGATCCCC 
TTCGGAGAGT GCTGTCCCAT CTGCCCAGCT GACCTCGCCA CTGCCAGTGG AAAATTAGGG 
CCAAAGGGGC AGAAAGGAGA ACCTGGAGAT ATCAGAGATA TCATAGGACC CAGAGGACCT 
CCTGGCCCTC AGGGACCTGC AGGTGAACAA GGACCCAGAG GTGATCGTGG TGACAAGGGA 
GAAAAGGGTG CGCCTGGACC CCGTGGCAGA GATGGAGAAC CTGGTACCCC TGGAAATCCT 
GGCCCCGCTG GCCCTCCAGG TCCCCCTGGT CCCCCTGGCC TTAGTGCAGG AAACTTCGCG 
GCTCAGATGG CTGGAGGGTA TGACGAGAAG GCTGGTGGTG CCCAGATGGG AGTCATGCAA 
GGGCCCATGG GCCCCATGGG ACCCCGTGGA CCCCCAGGCC CTGCCGGTGC CCCCGGCCCT 
CAAGGATTTC AAGGCAATCC TGGTGAACCT GGCGAGCCTG GTGTCTCTGG TCCCATGGGT 
CCCCGAGGTC CTCCTGGCCC TGCTGGAAAA CCTGGTGACG ACGGTGAAGC TGGGAAGCCC 
GGAAAGTCTG GGGAAAGAGG CCTCCCTGGC CCTCAGGGTG CTCGTGGATT CCCAGGAACC 
CCGGGTCTCC CCGGTGTCAA GGGTCACAGA GGTTACCCAG GCCTCGACGG TGCTAAGGGG 
GAAGCTGGTG CTCCGGGTGT GAAGGGTGAG AGTGGTTCCC CTGGTGAGAA CGGATCCCCG 
GGCCCAATGG GTCCCCGTGG CCTGCCTGGT GAGAGAGGAC GGACTGGCCC TGCTGGTGCT 
GCTGGTGCTC GGGGTAACGA TGGCCAGCCA GGCCCCGCTG GACCTCCGGG TCCTGTGGGT 
CCCGCAGGTG GTCCTGGCTT CCCTGGTGCT CCTGGTGCCA AGGGCGAAGC TGGTCCCACT 
GGTGCTCGCG GTCCTGAAGG TGCTCAAGGT TCTCGTGGCG AGCCTGGCAA TCCTGGGTCC 
CCTGGGCCTG CAGGTGCTTC TGGTAACCCA GGGACTGATG GTATTCCTGG AGCCAAAGGA 
TCCGCTGGTG CTCCTGGAAT TGCTGGTGCC CCTGGCTTCC CTGGGCCCCG TGGCCCTCCC 
GGTCCTCAAG GTGCAACTGG TCCCCTTGGC CCCAAAGGTC AGGCGGGTGA ACCTGGCATT 
GCTGGCTTTA AAGGTGATCA AGGCCCCAAG GGAGAGACTG GACCTGCTGG GCCCCAAGGA 
GCCCCTGGCC CCGCTGGTGA AGAAGGCAAA CGAGGTGCTC GAGGAGAGCC GGGTGGTGCT 
GGACCAATCG GACCCCCTGG AGAGAGAGGT GCTCCTGGCA ACCGTGGATT CCCAGGTCAA 
GATGGTCTGG CAGGTCCCAA GGGTGCCCCT GGAGAGCGAG GGCCCAGTGG CTTGACTGGT 
CCCAAGGGAG CCAACGGTGA CCCGGGTCGT CCTGGAGAAC CTGGTCTTCC TGGAGCCAGG 
GGTCTTACCG GTCGCCCTGG TGACGCTGGT CCTCAAGGCA AAGTTGGTCC TTCTGGAGCC 
CCTGGTGAAG ACGGTCGCCC TGGACCTCCT GGTCCTCAGG GAGCTCGTGG GCAGCCTGGC 
GTCATGGGTT TCCCTGGCCC CAAAGGTGCC AACGGCGAGC CTGGCAAAGC TGGTGAGAAG 
GGTCTGGCTG GCGCTCCTGG TCTGAGAGGT CTTCCTGGAA AAGACGGTGA GACGGGAGCC 
GCAGGACCCC CCGGCCCCAG TGGACCTGCT GGTGAACGAG GCGAGCAGGG CGCTCCTGGA 
CCATCAGGGT TCCAGGGACT TCCTGGCCCT CCCGGTCCCC CAGGTGAAGG TGGAAAGCAA 
GGTGACCAGG GTATTCCTGG TGAAGCTGGA GCTCCTGGCC TTGTGGGTCC TCGGGGCGAG 
CGAGGTTTCC CAGGTGAACG TGGCTCTCCT GGTGCTCAGG GCCTTCAGGG TCCCCGAGGC 
CTCCCTGGCA CTCCTGGTAC TGATGGTCCC AAAGGTGCAG CTGGCCCAGA TGGCCCCCCT 
GGGGCTCAGG GGCCTCCAGG TCTACAGGGA ATGCCTGGTG AGAGAGGAGC CGCTGGCATT 
GCTGGGCCCA AGGGAGACAG AGGCGATGTT GGCGAGAAAG GCCCAGAGGG AGCTCCTGGG 
AAGGATGGCG GCCGAGGTCT GACTGGGCCC ATCGGACCCC CAGGCCCAGC AGGGGCCAAC 
GGCGAGAAGG GAGAAGTCGG ACCTCCTGGC CCGTCAGGAA GTACCGGAGC TCGAGGTGCC 
CCGGGTGAAC GCGGAGAGAC CGGGCCACCT GGACCTGCTG GATTCGCTGG CCCTCCTGGT 
GCTGATGGCC AGCCTGGTGC CAAGGGTGAT CAAGGAGAAG CCGGACAGAA AGGAGATGCT 
GGTGCCCCCG GCCCACAAGG CCCCTCGGGA GCCCCTGGGC CACAGGGTCC TACTGGAGTG 
ACTGGTCCTA AGGGAGCCCG AGGTGCCCAA GGTCCCCCGG GAGCCACCGG ATTCCCTGGA 
GCTGCTGGCC GAGTTGGACC CCCAGGTGCT AATGGCAATC CTGGACCCGC CGGTCCCCCT 
GGTCCTGCTG GAAAAGATGG TCCCAAAGGT GTTCGAGGAG ACAGTGGCCC CCCTGGCAGA 
GCTGGTGACC CCGGTCTTCA AGGTCCTGCA GGAGCTCCTG GCGAGAAAGG AGAACCTGGA 
GATGATGGTC CCTCTGGTCT TGATGGTCCT CCAGGTCCCC AGGGGCTGGC TGGTCAAAGG 
GGCATTGTTG GTCTGCCTGG TCAGCGTGGT GAGAGAGGAT TCCCCGGCCT TCCCGGCCCA 
TCGGGTGAGC CCGGCAAGCA GGGTGCACCT GGCGCGTCTG GAGACAGAGG TCCTCCTGGT 
CCTGTGGGGC CTCCTGGCCT GACAGGGCCT GCAGGTGAAC CTGGACGAGA GGGCAGCCCT 
GGTGCTGATG GACCCCCTGG AAGAGATGGT GCAGCTGGAG TCAAGGGAGA TCGTGGTGAG 
ACTGGAGCAC TGGGTGCCCC TGGAGCTCCT GGGCCCCCAG GCTCTCCTGG TCCTGCTGGC 
CCAACTGGCA AACAAGGAGA CAGAGGAGAG GCTGGTGCAC AAGGTCCTAT GGGTCCCTCA 
GGACCTGCTG GAGCCCGTGG GATTGCAGGC CCTCAAGGCC CCCGAGGTGA CAAAGGAGAA 
TCTGGAGAGC AGGGCGAGAG GGGACTGAAG GGACACCGAG GTTTCACTGG ACTGCAGGGT 
CTGCCTGGCC CTCCGGGTCC TTCTGGAGAT CAGGGTGCTT CTGGCCCTGC TGGTCCTTCT 
GGCCCTAGAG GTCCACCTGG CCCTGTTGGT CCCTCTGGCA AAGATGGCTC TAATGGAATC 
CCTGGCCCCA TCGGGCCTCC AGGTCCCCGT GGACGCTCAG GAGAAACAGG CCCTGTTGGT 
CCCCCTGGAA GTCCCGGTCC TCCTGGCCCT CCAGGTCCTC CTGGTCCTGG CATCGACATG 
TCAGCCTTTG CTGGCTTAGG GCAGAGAGAG AAGGGCCCCG ACCCCATGCA GTACATGCGG 
GCCGACGAGG CAGACAGTAC CTTGAGACAG CACGACGTGG AGGTGGACGC TACACTCAAG 
TCACTGAACA ACCAGATTGA GAGCATCCGC AGCCCCGACG GCTCCCGCAA GAACCCTGCT 
CGCACTTGCC AAGACCTGAA ACTCTGCCAC CCCGAGTGGA AGAGCGGAGA CTACTGGATT 
GATCCCAACC AGGGCTGCAC CTTGGACGCC ATGAAAGTTT TCTGCAACAT GGAGACCGGC 
GAGACTTGCG TCTACCCCAA CCCAGCGACT GTCCCTCGGA AAAACTGGTG GAGCAGCAAG 
AGCAAGGAAA AGAAACACAT CTGGTTTGGA GAGACCATGA ACGGTGGCTT CCACTTCAGC 
TATGGCGATG GCAACCTGGC TCCCAACACC GCTAACGTCC AGATGACTTT CCTCCGTCTA 
CTGTCCACTG AGGGCTCCCA GAACATCACC TACCACTGTA AGAACAGCAT CGCCTACCTG 
GACGAAGCGG CTGGCAACCT CAAGAAGGCC TTGCTCATCC AGGGCTCCAA TGATGTAGAG 
ATGAGGGCCG AGGGCAACAG CAGGTTCACA TACACTGCCC TGAAGGATGG CTGCACGAAA 
CACACTGGTA AGTGGGGCAA GACCGTCATC GAGTACCGAT CACAGAAGAC CTCCCGCCTT 
CCCATTATTG ACATCGCACC CATGGACATT GGAGGGGCTG AACAGGAATT TGGTGTGGAC 
ATAGGGCCTG TCTGCTTCTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col2a1 ( XM_006520386.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520449.1 Mus musculus collagen, type II, alpha 1 (Col2a1), transcript variant X1, mRNA.

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CloneID	OMu50908
Clone ID Related Accession (Same CDS sequence)	XM_006520386.3
Accession Version	XM_006520386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(II) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520386.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAG GGTATGACGA GAAGGCTGGT GGTGCCCAGA TGGGAGTCAT GCAAGGGCCC 
ATGGGCCCCA TGGGACCCCG TGGACCCCCA GGCCCTGCCG GTGCCCCCGG CCCTCAAGGA 
TTTCAAGGCA ATCCTGGTGA ACCTGGCGAG CCTGGTGTCT CTGGTCCCAT GGGTCCCCGA 
GGTCCTCCTG GCCCTGCTGG AAAACCTGGT GACGACGGTG AAGCTGGGAA GCCCGGAAAG 
TCTGGGGAAA GAGGCCTCCC TGGCCCTCAG GGTGCTCGTG GATTCCCAGG AACCCCGGGT 
CTCCCCGGTG TCAAGGGTCA CAGAGGTTAC CCAGGCCTCG ACGGTGCTAA GGGGGAAGCT 
GGTGCTCCGG GTGTGAAGGG TGAGAGTGGT TCCCCTGGTG AGAACGGATC CCCGGGCCCA 
ATGGGTCCCC GTGGCCTGCC TGGTGAGAGA GGACGGACTG GCCCTGCTGG TGCTGCTGGT 
GCTCGGGGTA ACGATGGCCA GCCAGGCCCC GCTGGACCTC CGGGTCCTGT GGGTCCCGCA 
GGTGGTCCTG GCTTCCCTGG TGCTCCTGGT GCCAAGGGCG AAGCTGGTCC CACTGGTGCT 
CGCGGTCCTG AAGGTGCTCA AGGTTCTCGT GGCGAGCCTG GCAATCCTGG GTCCCCTGGG 
CCTGCAGGTG CTTCTGGTAA CCCAGGGACT GATGGTATTC CTGGAGCCAA AGGATCCGCT 
GGTGCTCCTG GAATTGCTGG TGCCCCTGGC TTCCCTGGGC CCCGTGGCCC TCCCGGTCCT 
CAAGGTGCAA CTGGTCCCCT TGGCCCCAAA GGTCAGGCGG GTGAACCTGG CATTGCTGGC 
TTTAAAGGTG ATCAAGGCCC CAAGGGAGAG ACTGGACCTG CTGGGCCCCA AGGAGCCCCT 
GGCCCCGCTG GTGAAGAAGG CAAACGAGGT GCTCGAGGAG AGCCGGGTGG TGCTGGACCA 
ATCGGACCCC CTGGAGAGAG AGGTGCTCCT GGCAACCGTG GATTCCCAGG TCAAGATGGT 
CTGGCAGGTC CCAAGGGTGC CCCTGGAGAG CGAGGGCCCA GTGGCTTGAC TGGTCCCAAG 
GGAGCCAACG GTGACCCGGG TCGTCCTGGA GAACCTGGTC TTCCTGGAGC CAGGGGTCTT 
ACCGGTCGCC CTGGTGACGC TGGTCCTCAA GGCAAAGTTG GTCCTTCTGG AGCCCCTGGT 
GAAGACGGTC GCCCTGGACC TCCTGGTCCT CAGGGAGCTC GTGGGCAGCC TGGCGTCATG 
GGTTTCCCTG GCCCCAAAGG TGCCAACGGC GAGCCTGGCA AAGCTGGTGA GAAGGGTCTG 
GCTGGCGCTC CTGGTCTGAG AGGTCTTCCT GGAAAAGACG GTGAGACGGG AGCCGCAGGA 
CCCCCCGGCC CCAGTGGACC TGCTGGTGAA CGAGGCGAGC AGGGCGCTCC TGGACCATCA 
GGGTTCCAGG GACTTCCTGG CCCTCCCGGT CCCCCAGGTG AAGGTGGAAA GCAAGGTGAC 
CAGGGTATTC CTGGTGAAGC TGGAGCTCCT GGCCTTGTGG GTCCTCGGGG CGAGCGAGGT 
TTCCCAGGTG AACGTGGCTC TCCTGGTGCT CAGGGCCTTC AGGGTCCCCG AGGCCTCCCT 
GGCACTCCTG GTACTGATGG TCCCAAAGGT GCAGCTGGCC CAGATGGCCC CCCTGGGGCT 
CAGGGGCCTC CAGGTCTACA GGGAATGCCT GGTGAGAGAG GAGCCGCTGG CATTGCTGGG 
CCCAAGGGAG ACAGAGGCGA TGTTGGCGAG AAAGGCCCAG AGGGAGCTCC TGGGAAGGAT 
GGCGGCCGAG GTCTGACTGG GCCCATCGGA CCCCCAGGCC CAGCAGGGGC CAACGGCGAG 
AAGGGAGAAG TCGGACCTCC TGGCCCGTCA GGAAGTACCG GAGCTCGAGG TGCCCCGGGT 
GAACGCGGAG AGACCGGGCC ACCTGGACCT GCTGGATTCG CTGGCCCTCC TGGTGCTGAT 
GGCCAGCCTG GTGCCAAGGG TGATCAAGGA GAAGCCGGAC AGAAAGGAGA TGCTGGTGCC 
CCCGGCCCAC AAGGCCCCTC GGGAGCCCCT GGGCCACAGG GTCCTACTGG AGTGACTGGT 
CCTAAGGGAG CCCGAGGTGC CCAAGGTCCC CCGGGAGCCA CCGGATTCCC TGGAGCTGCT 
GGCCGAGTTG GACCCCCAGG TGCTAATGGC AATCCTGGAC CCGCCGGTCC CCCTGGTCCT 
GCTGGAAAAG ATGGTCCCAA AGGTGTTCGA GGAGACAGTG GCCCCCCTGG CAGAGCTGGT 
GACCCCGGTC TTCAAGGTCC TGCAGGAGCT CCTGGCGAGA AAGGAGAACC TGGAGATGAT 
GGTCCCTCTG GTCTTGATGG TCCTCCAGGT CCCCAGGGGC TGGCTGGTCA AAGGGGCATT 
GTTGGTCTGC CTGGTCAGCG TGGTGAGAGA GGATTCCCCG GCCTTCCCGG CCCATCGGGT 
GAGCCCGGCA AGCAGGGTGC ACCTGGCGCG TCTGGAGACA GAGGTCCTCC TGGTCCTGTG 
GGGCCTCCTG GCCTGACAGG GCCTGCAGGT GAACCTGGAC GAGAGGGCAG CCCTGGTGCT 
GATGGACCCC CTGGAAGAGA TGGTGCAGCT GGAGTCAAGG GAGATCGTGG TGAGACTGGA 
GCACTGGGTG CCCCTGGAGC TCCTGGGCCC CCAGGCTCTC CTGGTCCTGC TGGCCCAACT 
GGCAAACAAG GAGACAGAGG AGAGGCTGGT GCACAAGGTC CTATGGGTCC CTCAGGACCT 
GCTGGAGCCC GTGGGATTGC AGGCCCTCAA GGCCCCCGAG GTGACAAAGG AGAATCTGGA 
GAGCAGGGCG AGAGGGGACT GAAGGGACAC CGAGGTTTCA CTGGACTGCA GGGTCTGCCT 
GGCCCTCCGG GTCCTTCTGG AGATCAGGGT GCTTCTGGCC CTGCTGGTCC TTCTGGCCCT 
AGAGGTCCAC CTGGCCCTGT TGGTCCCTCT GGCAAAGATG GCTCTAATGG AATCCCTGGC 
CCCATCGGGC CTCCAGGTCC CCGTGGACGC TCAGGAGAAA CAGGCCCTGT TGGTCCCCCT 
GGAAGTCCCG GTCCTCCTGG CCCTCCAGGT CCTCCTGGTC CTGGCATCGA CATGTCAGCC 
TTTGCTGGCT TAGGGCAGAG AGAGAAGGGC CCCGACCCCA TGCAGTACAT GCGGGCCGAC 
GAGGCAGACA GTACCTTGAG ACAGCACGAC GTGGAGGTGG ACGCTACACT CAAGTCACTG 
AACAACCAGA TTGAGAGCAT CCGCAGCCCC GACGGCTCCC GCAAGAACCC TGCTCGCACT 
TGCCAAGACC TGAAACTCTG CCACCCCGAG TGGAAGAGCG GAGACTACTG GATTGATCCC 
AACCAGGGCT GCACCTTGGA CGCCATGAAA GTTTTCTGCA ACATGGAGAC CGGCGAGACT 
TGCGTCTACC CCAACCCAGC GACTGTCCCT CGGAAAAACT GGTGGAGCAG CAAGAGCAAG 
GAAAAGAAAC ACATCTGGTT TGGAGAGACC ATGAACGGTG GCTTCCACTT CAGCTATGGC 
GATGGCAACC TGGCTCCCAA CACCGCTAAC GTCCAGATGA CTTTCCTCCG TCTACTGTCC 
ACTGAGGGCT CCCAGAACAT CACCTACCAC TGTAAGAACA GCATCGCCTA CCTGGACGAA 
GCGGCTGGCA ACCTCAAGAA GGCCTTGCTC ATCCAGGGCT CCAATGATGT AGAGATGAGG 
GCCGAGGGCA ACAGCAGGTT CACATACACT GCCCTGAAGG ATGGCTGCAC GAAACACACT 
GGTAAGTGGG GCAAGACCGT CATCGAGTAC CGATCACAGA AGACCTCCCG CCTTCCCATT 
ATTGACATCG CACCCATGGA CATTGGAGGG GCTGAACAGG AATTTGGTGT GGACATAGGG 
CCTGTCTGCT TCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520449.1
CDS	996..4913
Translation	MAGGYDEKAGGAQMGVMQGPMGPMGPRGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGLPGPQGARGFPGTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDGQPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGSRGEPGNPGSPGPAGASGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQAGEPGIAGFKGDQGPKGETGPAGPQGAPGPAGEEGKRGARGEPGGAGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLTGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAGAPGLRGLPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGDQGIPGEAGAPGLVGPRGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPPGAQGPPGLQGMPGERGAAGIAGPKGDRGDVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGPPGADGQPGAKGDQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGANGNPGPAGPPGPAGKDGPKGVRGDSGPPGRAGDPGLQGPAGAPGEKGEPGDDGPSGLDGPPGPQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGRDGAAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIAGPQGPRGDKGESGEQGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPGPRGRSGETGPVGPPGSPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPMQYMRADEADSTLRQHDVEVDATLKSLNNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPATVPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGAEQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	XM_006520386.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type II, alpha 1 (Col2a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520386.3

ORF Insert Sequence:

ATGGCTGGAG GGTATGACGA GAAGGCTGGT GGTGCCCAGA TGGGAGTCAT GCAAGGGCCC 
ATGGGCCCCA TGGGACCCCG TGGACCCCCA GGCCCTGCCG GTGCCCCCGG CCCTCAAGGA 
TTTCAAGGCA ATCCTGGTGA ACCTGGCGAG CCTGGTGTCT CTGGTCCCAT GGGTCCCCGA 
GGTCCTCCTG GCCCTGCTGG AAAACCTGGT GACGACGGTG AAGCTGGGAA GCCCGGAAAG 
TCTGGGGAAA GAGGCCTCCC TGGCCCTCAG GGTGCTCGTG GATTCCCAGG AACCCCGGGT 
CTCCCCGGTG TCAAGGGTCA CAGAGGTTAC CCAGGCCTCG ACGGTGCTAA GGGGGAAGCT 
GGTGCTCCGG GTGTGAAGGG TGAGAGTGGT TCCCCTGGTG AGAACGGATC CCCGGGCCCA 
ATGGGTCCCC GTGGCCTGCC TGGTGAGAGA GGACGGACTG GCCCTGCTGG TGCTGCTGGT 
GCTCGGGGTA ACGATGGCCA GCCAGGCCCC GCTGGACCTC CGGGTCCTGT GGGTCCCGCA 
GGTGGTCCTG GCTTCCCTGG TGCTCCTGGT GCCAAGGGCG AAGCTGGTCC CACTGGTGCT 
CGCGGTCCTG AAGGTGCTCA AGGTTCTCGT GGCGAGCCTG GCAATCCTGG GTCCCCTGGG 
CCTGCAGGTG CTTCTGGTAA CCCAGGGACT GATGGTATTC CTGGAGCCAA AGGATCCGCT 
GGTGCTCCTG GAATTGCTGG TGCCCCTGGC TTCCCTGGGC CCCGTGGCCC TCCCGGTCCT 
CAAGGTGCAA CTGGTCCCCT TGGCCCCAAA GGTCAGGCGG GTGAACCTGG CATTGCTGGC 
TTTAAAGGTG ATCAAGGCCC CAAGGGAGAG ACTGGACCTG CTGGGCCCCA AGGAGCCCCT 
GGCCCCGCTG GTGAAGAAGG CAAACGAGGT GCTCGAGGAG AGCCGGGTGG TGCTGGACCA 
ATCGGACCCC CTGGAGAGAG AGGTGCTCCT GGCAACCGTG GATTCCCAGG TCAAGATGGT 
CTGGCAGGTC CCAAGGGTGC CCCTGGAGAG CGAGGGCCCA GTGGCTTGAC TGGTCCCAAG 
GGAGCCAACG GTGACCCGGG TCGTCCTGGA GAACCTGGTC TTCCTGGAGC CAGGGGTCTT 
ACCGGTCGCC CTGGTGACGC TGGTCCTCAA GGCAAAGTTG GTCCTTCTGG AGCCCCTGGT 
GAAGACGGTC GCCCTGGACC TCCTGGTCCT CAGGGAGCTC GTGGGCAGCC TGGCGTCATG 
GGTTTCCCTG GCCCCAAAGG TGCCAACGGC GAGCCTGGCA AAGCTGGTGA GAAGGGTCTG 
GCTGGCGCTC CTGGTCTGAG AGGTCTTCCT GGAAAAGACG GTGAGACGGG AGCCGCAGGA 
CCCCCCGGCC CCAGTGGACC TGCTGGTGAA CGAGGCGAGC AGGGCGCTCC TGGACCATCA 
GGGTTCCAGG GACTTCCTGG CCCTCCCGGT CCCCCAGGTG AAGGTGGAAA GCAAGGTGAC 
CAGGGTATTC CTGGTGAAGC TGGAGCTCCT GGCCTTGTGG GTCCTCGGGG CGAGCGAGGT 
TTCCCAGGTG AACGTGGCTC TCCTGGTGCT CAGGGCCTTC AGGGTCCCCG AGGCCTCCCT 
GGCACTCCTG GTACTGATGG TCCCAAAGGT GCAGCTGGCC CAGATGGCCC CCCTGGGGCT 
CAGGGGCCTC CAGGTCTACA GGGAATGCCT GGTGAGAGAG GAGCCGCTGG CATTGCTGGG 
CCCAAGGGAG ACAGAGGCGA TGTTGGCGAG AAAGGCCCAG AGGGAGCTCC TGGGAAGGAT 
GGCGGCCGAG GTCTGACTGG GCCCATCGGA CCCCCAGGCC CAGCAGGGGC CAACGGCGAG 
AAGGGAGAAG TCGGACCTCC TGGCCCGTCA GGAAGTACCG GAGCTCGAGG TGCCCCGGGT 
GAACGCGGAG AGACCGGGCC ACCTGGACCT GCTGGATTCG CTGGCCCTCC TGGTGCTGAT 
GGCCAGCCTG GTGCCAAGGG TGATCAAGGA GAAGCCGGAC AGAAAGGAGA TGCTGGTGCC 
CCCGGCCCAC AAGGCCCCTC GGGAGCCCCT GGGCCACAGG GTCCTACTGG AGTGACTGGT 
CCTAAGGGAG CCCGAGGTGC CCAAGGTCCC CCGGGAGCCA CCGGATTCCC TGGAGCTGCT 
GGCCGAGTTG GACCCCCAGG TGCTAATGGC AATCCTGGAC CCGCCGGTCC CCCTGGTCCT 
GCTGGAAAAG ATGGTCCCAA AGGTGTTCGA GGAGACAGTG GCCCCCCTGG CAGAGCTGGT 
GACCCCGGTC TTCAAGGTCC TGCAGGAGCT CCTGGCGAGA AAGGAGAACC TGGAGATGAT 
GGTCCCTCTG GTCTTGATGG TCCTCCAGGT CCCCAGGGGC TGGCTGGTCA AAGGGGCATT 
GTTGGTCTGC CTGGTCAGCG TGGTGAGAGA GGATTCCCCG GCCTTCCCGG CCCATCGGGT 
GAGCCCGGCA AGCAGGGTGC ACCTGGCGCG TCTGGAGACA GAGGTCCTCC TGGTCCTGTG 
GGGCCTCCTG GCCTGACAGG GCCTGCAGGT GAACCTGGAC GAGAGGGCAG CCCTGGTGCT 
GATGGACCCC CTGGAAGAGA TGGTGCAGCT GGAGTCAAGG GAGATCGTGG TGAGACTGGA 
GCACTGGGTG CCCCTGGAGC TCCTGGGCCC CCAGGCTCTC CTGGTCCTGC TGGCCCAACT 
GGCAAACAAG GAGACAGAGG AGAGGCTGGT GCACAAGGTC CTATGGGTCC CTCAGGACCT 
GCTGGAGCCC GTGGGATTGC AGGCCCTCAA GGCCCCCGAG GTGACAAAGG AGAATCTGGA 
GAGCAGGGCG AGAGGGGACT GAAGGGACAC CGAGGTTTCA CTGGACTGCA GGGTCTGCCT 
GGCCCTCCGG GTCCTTCTGG AGATCAGGGT GCTTCTGGCC CTGCTGGTCC TTCTGGCCCT 
AGAGGTCCAC CTGGCCCTGT TGGTCCCTCT GGCAAAGATG GCTCTAATGG AATCCCTGGC 
CCCATCGGGC CTCCAGGTCC CCGTGGACGC TCAGGAGAAA CAGGCCCTGT TGGTCCCCCT 
GGAAGTCCCG GTCCTCCTGG CCCTCCAGGT CCTCCTGGTC CTGGCATCGA CATGTCAGCC 
TTTGCTGGCT TAGGGCAGAG AGAGAAGGGC CCCGACCCCA TGCAGTACAT GCGGGCCGAC 
GAGGCAGACA GTACCTTGAG ACAGCACGAC GTGGAGGTGG ACGCTACACT CAAGTCACTG 
AACAACCAGA TTGAGAGCAT CCGCAGCCCC GACGGCTCCC GCAAGAACCC TGCTCGCACT 
TGCCAAGACC TGAAACTCTG CCACCCCGAG TGGAAGAGCG GAGACTACTG GATTGATCCC 
AACCAGGGCT GCACCTTGGA CGCCATGAAA GTTTTCTGCA ACATGGAGAC CGGCGAGACT 
TGCGTCTACC CCAACCCAGC GACTGTCCCT CGGAAAAACT GGTGGAGCAG CAAGAGCAAG 
GAAAAGAAAC ACATCTGGTT TGGAGAGACC ATGAACGGTG GCTTCCACTT CAGCTATGGC 
GATGGCAACC TGGCTCCCAA CACCGCTAAC GTCCAGATGA CTTTCCTCCG TCTACTGTCC 
ACTGAGGGCT CCCAGAACAT CACCTACCAC TGTAAGAACA GCATCGCCTA CCTGGACGAA 
GCGGCTGGCA ACCTCAAGAA GGCCTTGCTC ATCCAGGGCT CCAATGATGT AGAGATGAGG 
GCCGAGGGCA ACAGCAGGTT CACATACACT GCCCTGAAGG ATGGCTGCAC GAAACACACT 
GGTAAGTGGG GCAAGACCGT CATCGAGTAC CGATCACAGA AGACCTCCCG CCTTCCCATT 
ATTGACATCG CACCCATGGA CATTGGAGGG GCTGAACAGG AATTTGGTGT GGACATAGGG 
CCTGTCTGCT TCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Col2a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

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会社情報

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