Col14a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col14a1
Entrez Gene ID	12818
Full Name	collagen, type XIV, alpha 1
Synonyms	5730412L22Rik,AW108078
General protein information	Preferred Names collagen, type XIV, alpha 1 Names collagen alpha-1(XIV) chain col14a1 procollagen, type XIV, alpha 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_181277.3	NP_851794.3	collagen alpha-1(XIV) chain precursor
NM_001368422.1	NP_001355351.1	collagen alpha-1(XIV) chain isoform 1 precursor
XM_006520384.3	XP_006520447.1	collagen alpha-1(XIV) chain isoform X1
XM_006520385.3	XP_006520448.1	collagen alpha-1(XIV) chain isoform X2
NM_181277.4	NP_851794.3	collagen alpha-1(XIV) chain isoform 2 precursor
XM_006520385.4	XP_006520448.1	collagen alpha-1(XIV) chain isoform X1

Pathways from BioSystems

Homologies

	COL14A1	XP_002805526.1
Gallus gallus (chicken)	COL14A1	NP_990665.1
Danio rerio (zebrafish)	col14a1a	XP_001922046.4
Homo sapiens (human)	COL14A1	NP_066933.1
Canis lupus familiaris (dog)	COL14A1	XP_532319.2
Rattus norvegicus (Norway rat)	Col14a1	NP_001124020.1
Pan troglodytes (chimpanzee)	COL14A1	XP_519927.2
Bos taurus (cattle)	COL14A1	XP_003586965.2
Mus musculus (house mouse)	Col14a1	NP_851794.3

Gene Ontology
Bibliography

Related articles in PubMed

Odd skipped-related 1 identifies a population of embryonic fibro-adipogenic progenitors regulating myogenesis during limb development.
Vallecillo-Garc?a P, Orgeur M, Vom Hofe-Schneider S, Stumm J, Kappert V, Ibrahim DM, B?rno ST, Hayashi S, Relaix F, Hildebrandt K, Sengle G, Koch M, Timmermann B, Marazzi G, Sassoon DA, Duprez D, Stricker S
Nature communications8(1)1218(2017 Oct)

Scleraxis is required for maturation of tissue domains for proper integration of the musculoskeletal system.
Yoshimoto Y, Takimoto A, Watanabe H, Hiraki Y, Kondoh G, Shukunami C
Scientific reports745010(2017 Mar)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

Regulators of Collagen Fibrillogenesis during Molar Development in the Mouse.
Zvackova I, Matalova E, Lesot H
Frontiers in physiology8554(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Col14a1 deficiency leads to a greater increase in left ventricular wall thickening in response to pathological pressure overload compared to wild type animals.
Title: Collagen XIV is important for growth and structural integrity of the myocardium.

a regulatory function for type XIV collagen in early stages of collagen fibrillogenesis with tissue differences
Title: Type XIV Collagen Regulates Fibrillogenesis: PREMATURE COLLAGEN FIBRIL GROWTH AND TISSUE DYSFUNCTION IN NULL MICE.

the fibronectin type III domain of collagen XIV is an inducer of quiescence and differentiation in fibroblasts and preadipocytes
Title: The elongated first fibronectin type III domain of collagen XIV is an inducer of quiescence and differentiation in fibroblasts and preadipocytes.

The 6456 nucleotide (nt) cDNA contains 5391-nt open reading frame encoding 1797 amino acid residues. Amino terminus has a28-residue signal peptide followed by mature polypeptide of 1769 amino acid residues with calculated molecular mass of 193.2 kDa.
Title: Complete primary structure and genomic organization of the mouse Col14a1 gene.

The entire mouse cDNA sequence for type XIV collagen was determined using overlapping PCR products. The 6456 nucleotide (nt) cDNA sequence contains a 5391-nt open reading frame encoding 1797 amino acid residues.
Title: Complete primary structure and genomic organization of the mouse Col14a1 gene.

The following Col14a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col14a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu50907	XM_006520385.4 Latest version!	Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu07946	NM_181277.4 Latest version!	Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50906	NM_001368422.1 Latest version!	Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50906	XM_006520384.3 Latest version!	Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu07946	NM_181277.3	Mus musculus collagen, type XIV, alpha 1 (Col14a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50907	XM_006520385.3	Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col14a1 ( XM_006520385.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520448.1 Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant X1, mRNA.

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CloneID	OMu50907
Clone ID Related Accession (Same CDS sequence)	XM_006520385.3 , XM_006520385.4
Accession Version	XM_006520385.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XIV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520385.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTAGGAA CGGTTATGGT GCCTGGAGTG CAAAACAGTC TCCTTCTGAA GGCTCTACTT 
CCAGACACTG AGTATAAAGT CACAGTAACC CCTGTCTATA CTGTTGGAGA AGGCGTCAGC 
GTCTCCGCTC CAGGGAAGAC TTTGCCCTCC TCTGGTCCTC AAAACCTGAG GGTCTCAGAA 
GAATGGTACA ACCGGGTGCG CATTACATGG GACCCACCCT CTGGCCCCGT GAAGGGCTAT 
AGGATTGTCT ACAAACCTGT GAGTGTCCCT GGTCAAACTC TGGAGACCTT CGTGGGTGCT 
GACATCAACA CCATTGTCAT GACAAATCTC CTCAGTGGAA TGGACTACAA TGTGAAGATC 
TTTGCTTCCC AGGCGTCAGG CTTCAGTGAT GCTCTGACTG GCCTCGTGCA AACACTGTTC 
TTAGGGGTGA CAGATCTCCA AGCCAATCAG GTTGAAATGA CAAGCTTGTG TGCTCGGTGG 
CAAATACATC GCCATGCCAC AGCCTACAGG ATAGTTCTAG AATCCCTTCA GGACACACAA 
GCACAGGAAT CCACGGTGGG AGGAGGCGTA AACAGGCATT GCTTCTATGG ACTTCAGCCT 
GATTCAGAGT ATAAAATCAG TGTTTACACA AAGCTCCAGG AGCTAGAGGG ACCCAGTGTG 
AGCATCATGC AGAAAACACA GTCACTTCCC ACGGAACCAC CGACTTTTCC TCCAACCATC 
CCACCAGCAA AGGAAGTATG TAAAGCTGCC AAGGCAGACC TGGTATTCAT GGTGGATGGA 
TCTTGGAGCA TAGGAGATGA CAATTTCAAT AAAATCATCA ATTTTCTATA CAGCACTGTG 
GGAGCCCTGG ACAAGATTGG TGCAGATGGA ACTCAGGTGG CAATGGTTCA GTTCACTGAT 
GACCCCCGAA CAGAATTCAA ACTTGATTCT TACAAAACCA AAGAGACCCT TCTTGATGCA 
ATTAGACACA TTTCCTACAA AGGAGGAAAT ACTAAAACAG GAAAAGCTAT CAAGCATGTT 
CGGGATACCT TATTTACATC AGACTCAGGG ACAAGGAGAG GCATCCCAAA GGTCATCGTG 
GTGATAACTG ATGGACGGTC ACAAGATGAT GTCAACAAAA TCTCCAGGGA GATGCAGGCA 
GATGGTTTCA ACATCTTTGC CATCGGTGTG GCTGACGCTG ACTACTCCGA GTTGGTTCAG 
ATCGGCAGCA AGCCCAGCTC ACGGCATGTC TTCTTTGTGG ATGACTTTGA TGCCTTTAAG 
AAAATTGAAG ATGAATTGAT TACTTTTGTC TGTGAAACTG CATCAGCAAC CTGCCCAATG 
GTGCACAAGG ATGGAGTTGA TCTTGCAGGG TTTAAGATGA TGGAAATGTT TGGTTTGGTT 
GAAAAGGACT TTTCTGCAGT GGAAGGAGTA TCTATGGAAC CTGGTACCTT CAATTTGTTC 
CCATGCTATC AAATCCATAA GGATGCTCTG GTTTCTCAGC CAACAAAGTA TCTGCACCCA 
GAAGGATTGC CTTCTGACTA TACAATGAGT TTCCTGTTCC GGATTCTTCC TGACACACCA 
CAGGAGCCAT TTGCTCTTTG GGAGATTTTA AACAAAAATT CTGAGCCGCT AGTTGGGATC 
ATATTGGACA ATGGTGGGAA AACTCTAACT TATTTCAACT ATGACTATAC TGGAGATTTT 
CAAACTGTCA CTTTTGAAGG ACCCGACATC AGGAAAATGT TCTATGGAAG CTTTCACAAG 
CTCCATGTTG TTGTCAGCAA GACTCTGGCC AAAGTGGTTG TTGACTGCAA GGAAGTTGGT 
CAGAAGGCAA TAAATGCATC AGCGAATATC ACATCGGATG GTGTGGAAGT ACTAGGAAGA 
ATGGTCAGAT CAAGAGGACC AAATGGAAAC TCTGCACCAT TCCAGTTACA AATGTTTGAT 
ATCGTTTGCT CTACATCATG GGCCAGTAAA GACAGATGCT GCGAACTTCC TGGTCTGCGG 
GATGAAGAGT CCTGCCCAGA CCTTCCACGG TCCTGCTCTT GCTCGGAAAC CAATGAAGTG 
GCTCTAGGAC CAGCTGGCCC CCCAGGTGGT CCAGGACTCC GAGGACCAAA AGGCCAACAA 
GGTGAACAGG GTCCGAAGGG ACCAGAGGGA CCTCGGGGAG AAACAGGTCC TGCAGGACCC 
CAGGGCCCCC CTGGACCCCA GGGACCTAGT GGTCTGTCCA TTCAAGGCAT GCCTGGAATG 
CCGGGTGATA AAGGAGATAA AGGAGATGCT GGTCTTCCTG GTCCACAGGG TGTCCCTGGA 
GGTGTTGGTT CACCAGGCCG TGATGGCTCA CCAGGCCAGA GGGGATTTCC CGGGAAGGAC 
GGTTCCTCTG GGCCTCCAGG ACCACCTGGG CCAATTGGCA TTCCTGGAGC CCCTGGTGTC 
CCAGGGATCA CAGGCAGCAT GGGACCGCAA GGAGCCCTGG GGCCACCTGG TGTCCCTGGA 
GCGAAGGGAG AGCGAGGTGA ACGGGGAGAC CTGCAGTCTC AAGCCATGGT GAGGGCGGTG 
GCACGTCAAG TATGCGAGCA GCTCATTCAG AGTCACATGG CCAGGTACAC AGCCATCCTC 
AACCAGATCC CCAGCCAGTC CTCATCTATC CGGACCATCC AGGGCCCTCC TGGGGAGCCC 
GGGAGACCAG GTTCACCTGG AACCCCTGGA GAACAAGGAC CCCCAGGTAC CCCAGGCTTC 
CCAGGAAATG CAGGAGTTCC GGGGACCCCA GGAGAACGAG GTCTAACTGG TGTCAAAGGA 
GAGAAAGGAA ATCCAGGCAT TGGAACTCAA GGCCCAAGAG GTCCCCCTGG GCCAGCAGGA 
CCTTCAGGGG AAAGTCGTCC TGGAAGTCCT GGGCCACCTG GCTCTCCTGG ACCAAGGGGT 
CCCCCGGGCC ACCTGGGAGT ACCTGGACCT CAGGGTCCTT CTGGCCAGCC TGGGTATTGT 
GACCCCTCCT CGTGCTCTGC TTATGGCGTT GGAGATCTCA TCCCGTACAA TGATTACCAG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520448.1
CDS	379..5724
Translation	MMIWQCKMRDWLILAFLAAACFCTIVRGQVAPPTRLRYNVISHDSIQISWKAPRGKFGGYKLLVAPASGGKTNQMNLQNTATKAIIQGLLPEQNYTVQLIAYYKDKESKPAQGQFRIKDLEKRKDPTKPKVKVVDKGNGSKPTSPEEVKFFCETPAIADIVILVDGSWSIGRFNFRLVRNFLENLVTAFNVGSEKTRIGLAQYSGDPRIEWHLNAFNTKDEVIDAVRSLPYKGGNTLTGLALNFIFENSFKPEAGSRSGVSKIGILITDGKSQDDIIPPSRNLRESGVELFAIGVKNADLSELQEIASEPDSTHVYNVAEFDLMHTVVESLTRTVCSRVEEQDKEIKASALATIGPPTELITSEVTARSFMVNWTQSPGKVEKYRVVYYPTRGGKPEEVVVDGSVSSTVLKNLMSSTEYQIAVFAVSAHTASEGLRGAETTLALPMASDLELYDVTENSMRVRWDAVPGATGYLILYAPLTEGLAGDEKEMKIGETHTDIELSGLFPNTEYTVTVYAMFGEEASDPATGQETTLPLTPPRNLRISNVGSNSARLTWDPASGKISGYRIVYTSADGTEINEVEVDPITTFPLKGLTPLTEYSIAIFSIYEEGQSLPLVGEFTTEEVPAQQYLEIDEVKTDSFRVTWHPLSAEEGQHKLMWIPVYGGKTQEVDLKEEQDSYVIEGLDPGTEYEVSLLAVLDDGSESEVVTAVGTTLDDFWTEAPTAIEPTSPVTSVLQTGIRNLVVDDETATSLRVSWDISDSNVEQFRVTYLKAQGDPMEEVVGTVMVPGVQNSLLLKALLPDTEYKVTVTPVYTVGEGVSVSAPGKTLPSSGPQNLRVSEEWYNRVRITWDPPSGPVKGYRIVYKPVSVPGQTLETFVGADINTIVMTNLLSGMDYNVKIFASQASGFSDALTGLVQTLFLGVTDLQANQVEMTSLCARWQIHRHATAYRIVLESLQDTQAQESTVGGGVNRHCFYGLQPDSEYKISVYTKLQELEGPSVSIMQKTQSLPTEPPTFPPTIPPAKEVCKAAKADLVFMVDGSWSIGDDNFNKIINFLYSTVGALDKIGADGTQVAMVQFTDDPRTEFKLDSYKTKETLLDAIRHISYKGGNTKTGKAIKHVRDTLFTSDSGTRRGIPKVIVVITDGRSQDDVNKISREMQADGFNIFAIGVADADYSELVQIGSKPSSRHVFFVDDFDAFKKIEDELITFVCETASATCPMVHKDGVDLAGFKMMEMFGLVEKDFSAVEGVSMEPGTFNLFPCYQIHKDALVSQPTKYLHPEGLPSDYTMSFLFRILPDTPQEPFALWEILNKNSEPLVGIILDNGGKTLTYFNYDYTGDFQTVTFEGPDIRKMFYGSFHKLHVVVSKTLAKVVVDCKEVGQKAINASANITSDGVEVLGRMVRSRGPNGNSAPFQLQMFDIVCSTSWASKDRCCELPGLRDEESCPDLPRSCSCSETNEVALGPAGPPGGPGLRGPKGQQGEQGPKGPEGPRGETGPAGPQGPPGPQGPSGLSIQGMPGMPGDKGDKGDAGLPGPQGVPGGVGSPGRDGSPGQRGFPGKDGSSGPPGPPGPIGIPGAPGVPGITGSMGPQGALGPPGVPGAKGERGERGDLQSQAMVRAVARQVCEQLIQSHMARYTAILNQIPSQSSSIRTIQGPPGEPGRPGSPGTPGEQGPPGTPGFPGNAGVPGTPGERGLTGVKGEKGNPGIGTQGPRGPPGPAGPSGESRPGSPGPPGSPGPRGPPGHLGVPGPQGPSGQPGYCDPSSCSAYGVGDLIPYNDYQH

Target ORF information:

RefSeq Version	XM_006520385.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520385.4

ORF Insert Sequence:

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTAGGAA CGGTTATGGT GCCTGGAGTG CAAAACAGTC TCCTTCTGAA GGCTCTACTT 
CCAGACACTG AGTATAAAGT CACAGTAACC CCTGTCTATA CTGTTGGAGA AGGCGTCAGC 
GTCTCCGCTC CAGGGAAGAC TTTGCCCTCC TCTGGTCCTC AAAACCTGAG GGTCTCAGAA 
GAATGGTACA ACCGGGTGCG CATTACATGG GACCCACCCT CTGGCCCCGT GAAGGGCTAT 
AGGATTGTCT ACAAACCTGT GAGTGTCCCT GGTCAAACTC TGGAGACCTT CGTGGGTGCT 
GACATCAACA CCATTGTCAT GACAAATCTC CTCAGTGGAA TGGACTACAA TGTGAAGATC 
TTTGCTTCCC AGGCGTCAGG CTTCAGTGAT GCTCTGACTG GCCTCGTGCA AACACTGTTC 
TTAGGGGTGA CAGATCTCCA AGCCAATCAG GTTGAAATGA CAAGCTTGTG TGCTCGGTGG 
CAAATACATC GCCATGCCAC AGCCTACAGG ATAGTTCTAG AATCCCTTCA GGACACACAA 
GCACAGGAAT CCACGGTGGG AGGAGGCGTA AACAGGCATT GCTTCTATGG ACTTCAGCCT 
GATTCAGAGT ATAAAATCAG TGTTTACACA AAGCTCCAGG AGCTAGAGGG ACCCAGTGTG 
AGCATCATGC AGAAAACACA GTCACTTCCC ACGGAACCAC CGACTTTTCC TCCAACCATC 
CCACCAGCAA AGGAAGTATG TAAAGCTGCC AAGGCAGACC TGGTATTCAT GGTGGATGGA 
TCTTGGAGCA TAGGAGATGA CAATTTCAAT AAAATCATCA ATTTTCTATA CAGCACTGTG 
GGAGCCCTGG ACAAGATTGG TGCAGATGGA ACTCAGGTGG CAATGGTTCA GTTCACTGAT 
GACCCCCGAA CAGAATTCAA ACTTGATTCT TACAAAACCA AAGAGACCCT TCTTGATGCA 
ATTAGACACA TTTCCTACAA AGGAGGAAAT ACTAAAACAG GAAAAGCTAT CAAGCATGTT 
CGGGATACCT TATTTACATC AGACTCAGGG ACAAGGAGAG GCATCCCAAA GGTCATCGTG 
GTGATAACTG ATGGACGGTC ACAAGATGAT GTCAACAAAA TCTCCAGGGA GATGCAGGCA 
GATGGTTTCA ACATCTTTGC CATCGGTGTG GCTGACGCTG ACTACTCCGA GTTGGTTCAG 
ATCGGCAGCA AGCCCAGCTC ACGGCATGTC TTCTTTGTGG ATGACTTTGA TGCCTTTAAG 
AAAATTGAAG ATGAATTGAT TACTTTTGTC TGTGAAACTG CATCAGCAAC CTGCCCAATG 
GTGCACAAGG ATGGAGTTGA TCTTGCAGGG TTTAAGATGA TGGAAATGTT TGGTTTGGTT 
GAAAAGGACT TTTCTGCAGT GGAAGGAGTA TCTATGGAAC CTGGTACCTT CAATTTGTTC 
CCATGCTATC AAATCCATAA GGATGCTCTG GTTTCTCAGC CAACAAAGTA TCTGCACCCA 
GAAGGATTGC CTTCTGACTA TACAATGAGT TTCCTGTTCC GGATTCTTCC TGACACACCA 
CAGGAGCCAT TTGCTCTTTG GGAGATTTTA AACAAAAATT CTGAGCCGCT AGTTGGGATC 
ATATTGGACA ATGGTGGGAA AACTCTAACT TATTTCAACT ATGACTATAC TGGAGATTTT 
CAAACTGTCA CTTTTGAAGG ACCCGACATC AGGAAAATGT TCTATGGAAG CTTTCACAAG 
CTCCATGTTG TTGTCAGCAA GACTCTGGCC AAAGTGGTTG TTGACTGCAA GGAAGTTGGT 
CAGAAGGCAA TAAATGCATC AGCGAATATC ACATCGGATG GTGTGGAAGT ACTAGGAAGA 
ATGGTCAGAT CAAGAGGACC AAATGGAAAC TCTGCACCAT TCCAGTTACA AATGTTTGAT 
ATCGTTTGCT CTACATCATG GGCCAGTAAA GACAGATGCT GCGAACTTCC TGGTCTGCGG 
GATGAAGAGT CCTGCCCAGA CCTTCCACGG TCCTGCTCTT GCTCGGAAAC CAATGAAGTG 
GCTCTAGGAC CAGCTGGCCC CCCAGGTGGT CCAGGACTCC GAGGACCAAA AGGCCAACAA 
GGTGAACAGG GTCCGAAGGG ACCAGAGGGA CCTCGGGGAG AAACAGGTCC TGCAGGACCC 
CAGGGCCCCC CTGGACCCCA GGGACCTAGT GGTCTGTCCA TTCAAGGCAT GCCTGGAATG 
CCGGGTGATA AAGGAGATAA AGGAGATGCT GGTCTTCCTG GTCCACAGGG TGTCCCTGGA 
GGTGTTGGTT CACCAGGCCG TGATGGCTCA CCAGGCCAGA GGGGATTTCC CGGGAAGGAC 
GGTTCCTCTG GGCCTCCAGG ACCACCTGGG CCAATTGGCA TTCCTGGAGC CCCTGGTGTC 
CCAGGGATCA CAGGCAGCAT GGGACCGCAA GGAGCCCTGG GGCCACCTGG TGTCCCTGGA 
GCGAAGGGAG AGCGAGGTGA ACGGGGAGAC CTGCAGTCTC AAGCCATGGT GAGGGCGGTG 
GCACGTCAAG TATGCGAGCA GCTCATTCAG AGTCACATGG CCAGGTACAC AGCCATCCTC 
AACCAGATCC CCAGCCAGTC CTCATCTATC CGGACCATCC AGGGCCCTCC TGGGGAGCCC 
GGGAGACCAG GTTCACCTGG AACCCCTGGA GAACAAGGAC CCCCAGGTAC CCCAGGCTTC 
CCAGGAAATG CAGGAGTTCC GGGGACCCCA GGAGAACGAG GTCTAACTGG TGTCAAAGGA 
GAGAAAGGAA ATCCAGGCAT TGGAACTCAA GGCCCAAGAG GTCCCCCTGG GCCAGCAGGA 
CCTTCAGGGG AAAGTCGTCC TGGAAGTCCT GGGCCACCTG GCTCTCCTGG ACCAAGGGGT 
CCCCCGGGCC ACCTGGGAGT ACCTGGACCT CAGGGTCCTT CTGGCCAGCC TGGGTATTGT 
GACCCCTCCT CGTGCTCTGC TTATGGCGTT GGAGATCTCA TCCCGTACAA TGATTACCAG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col14a1 ( NM_181277.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_851794.3 Mus musculus collagen, type XIV, alpha 1 (Col14a1), mRNA.

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CloneID	OMu07946
Clone ID Related Accession (Same CDS sequence)	NM_181277.3 , NM_181277.4
Accession Version	NM_181277.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-11
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XIV) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK017553.1, AW259216.1, BC138345.1 and CF582947.1. On Apr 7, 2009 this sequence version replaced NM_181277.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138345.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTTATGG TGCCTGGAGT GCAAAACAGT CTCCTTCTGA AGGCTCTACT TCCAGACACT 
GAGTATAAAG TCACAGTAAC CCCTGTCTAT ACTGTTGGAG AAGGCGTCAG CGTCTCCGCT 
CCAGGGAAGA CTTTGCCCTC CTCTGGTCCT CAAAACCTGA GGGTCTCAGA AGAATGGTAC 
AACCGGGTGC GCATTACATG GGACCCACCC TCTGGCCCCG TGAAGGGCTA TAGGATTGTC 
TACAAACCTG TGAGTGTCCC TGGTCAAACT CTGGAGACCT TCGTGGGTGC TGACATCAAC 
ACCATTGTCA TGACAAATCT CCTCAGTGGA ATGGACTACA ATGTGAAGAT CTTTGCTTCC 
CAGGCGTCAG GCTTCAGTGA TGCTCTGACT GGCCTCGTGC AAACACTGTT CTTAGGGGTG 
ACAGATCTCC AAGCCAATCA GGTTGAAATG ACAAGCTTGT GTGCTCGGTG GCAAATACAT 
CGCCATGCCA CAGCCTACAG GATAGTTCTA GAATCCCTTC AGGACACACA AGCACAGGAA 
TCCACGGTGG GAGGAGGCGT AAACAGGCAT TGCTTCTATG GACTTCAGCC TGATTCAGAG 
TATAAAATCA GTGTTTACAC AAAGCTCCAG GAGCTAGAGG GACCCAGTGT GAGCATCATG 
CAGAAAACAC AGTCACTTCC CACGGAACCA CCGACTTTTC CTCCAACCAT CCCACCAGCA 
AAGGAAGTAT GTAAAGCTGC CAAGGCAGAC CTGGTATTCA TGGTGGATGG ATCTTGGAGC 
ATAGGAGATG ACAATTTCAA TAAAATCATC AATTTTCTAT ACAGCACTGT GGGAGCCCTG 
GACAAGATTG GTGCAGATGG AACTCAGGTG GCAATGGTTC AGTTCACTGA TGACCCCCGA 
ACAGAATTCA AACTTGATTC TTACAAAACC AAAGAGACCC TTCTTGATGC AATTAGACAC 
ATTTCCTACA AAGGAGGAAA TACTAAAACA GGAAAAGCTA TCAAGCATGT TCGGGATACC 
TTATTTACAT CAGACTCAGG GACAAGGAGA GGCATCCCAA AGGTCATCGT GGTGATAACT 
GATGGACGGT CACAAGATGA TGTCAACAAA ATCTCCAGGG AGATGCAGGC AGATGGTTTC 
AACATCTTTG CCATCGGTGT GGCTGACGCT GACTACTCCG AGTTGGTTCA GATCGGCAGC 
AAGCCCAGCT CACGGCATGT CTTCTTTGTG GATGACTTTG ATGCCTTTAA GAAAATTGAA 
GATGAATTGA TTACTTTTGT CTGTGAAACT GCATCAGCAA CCTGCCCAAT GGTGCACAAG 
GATGGAGTTG ATCTTGCAGG GTTTAAGATG ATGGAAATGT TTGGTTTGGT TGAAAAGGAC 
TTTTCTGCAG TGGAAGGAGT ATCTATGGAA CCTGGTACCT TCAATTTGTT CCCATGCTAT 
CAAATCCATA AGGATGCTCT GGTTTCTCAG CCAACAAAGT ATCTGCACCC AGAAGGATTG 
CCTTCTGACT ATACAATGAG TTTCCTGTTC CGGATTCTTC CTGACACACC ACAGGAGCCA 
TTTGCTCTTT GGGAGATTTT AAACAAAAAT TCTGAGCCGC TAGTTGGGAT CATATTGGAC 
AATGGTGGGA AAACTCTAAC TTATTTCAAC TATGACTATA CTGGAGATTT TCAAACTGTC 
ACTTTTGAAG GACCCGACAT CAGGAAAATG TTCTATGGAA GCTTTCACAA GCTCCATGTT 
GTTGTCAGCA AGACTCTGGC CAAAGTGGTT GTTGACTGCA AGGAAGTTGG TCAGAAGGCA 
ATAAATGCAT CAGCGAATAT CACATCGGAT GGTGTGGAAG TACTAGGAAG AATGGTCAGA 
TCAAGAGGAC CAAATGGAAA CTCTGCACCA TTCCAGTTAC AAATGTTTGA TATCGTTTGC 
TCTACATCAT GGGCCAGTAA AGACAGATGC TGCGAACTTC CTGGTCTGCG GGATGAAGAG 
TCCTGCCCAG ACCTTCCACG GTCCTGCTCT TGCTCGGAAA CCAATGAAGT GGCTCTAGGA 
CCAGCTGGCC CCCCAGGTGG TCCAGGACTC CGAGGACCAA AAGGCCAACA AGGTGAACAG 
GGTCCGAAGG GACCAGAGGG ACCTCGGGGA GAAACAGGTC CTGCAGGACC CCAGGGCCCC 
CCTGGACCCC AGGGACCTAG TGGTCTGTCC ATTCAAGGCA TGCCTGGAAT GCCGGGTGAT 
AAAGGAGATA AAGGAGATGC TGGTCTTCCT GGTCCACAGG GTGTCCCTGG AGGTGTTGGT 
TCACCAGGCC GTGATGGCTC ACCAGGCCAG AGGGGATTTC CCGGGAAGGA CGGTTCCTCT 
GGGCCTCCAG GACCACCTGG GCCAATTGGC ATTCCTGGAG CCCCTGGTGT CCCAGGGATC 
ACAGGCAGCA TGGGACCGCA AGGAGCCCTG GGGCCACCTG GTGTCCCTGG AGCGAAGGGA 
GAGCGAGGTG AACGGGGAGA CCTGCAGTCT CAAGCCATGG TGAGGGCGGT GGCACGTCAA 
GTATGCGAGC AGCTCATTCA GAGTCACATG GCCAGGTACA CAGCCATCCT CAACCAGATC 
CCCAGCCAGT CCTCATCTAT CCGGACCATC CAGGGCCCTC CTGGGGAGCC CGGGAGACCA 
GGTTCACCTG GAACCCCTGG AGAACAAGGA CCCCCAGGTA CCCCAGGCTT CCCAGGAAAT 
GCAGGAGTTC CGGGGACCCC AGGAGAACGA GGTCTAACTG GTGTCAAAGG AGAGAAAGGA 
AATCCAGGCA TTGGAACTCA AGGCCCAAGA GGTCCCCCTG GGCCAGCAGG ACCTTCAGGG 
GAAAGTCGTC CTGGAAGTCC TGGGCCACCT GGCTCTCCTG GACCAAGGGG TCCCCCGGGC 
CACCTGGGAG TACCTGGACC TCAGGGTCCT TCTGGCCAGC CTGGGTATTG TGACCCCTCC 
TCGTGCTCTG CTTATGGCGT TGGAGTGTCC CATCCTGATC AGCCTGAGTT CACACCGGTC 
CAGGACGAGC AGGAGGCCAT GGATCTGTGG AGCGCAGGCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851794.3
CDS	273..5657
Translation	MMIWQCKMRDWLILAFLAAACFCTIVRGQVAPPTRLRYNVISHDSIQISWKAPRGKFGGYKLLVAPASGGKTNQMNLQNTATKAIIQGLLPEQNYTVQLIAYYKDKESKPAQGQFRIKDLEKRKDPTKPKVKVVDKGNGSKPTSPEEVKFFCETPAIADIVILVDGSWSIGRFNFRLVRNFLENLVTAFNVGSEKTRIGLAQYSGDPRIEWHLNAFNTKDEVIDAVRSLPYKGGNTLTGLALNFIFENSFKPEAGSRSGVSKIGILITDGKSQDDIIPPSRNLRESGVELFAIGVKNADLSELQEIASEPDSTHVYNVAEFDLMHTVVESLTRTVCSRVEEQDKEIKASALATIGPPTELITSEVTARSFMVNWTQSPGKVEKYRVVYYPTRGGKPEEVVVDGSVSSTVLKNLMSSTEYQIAVFAVSAHTASEGLRGAETTLALPMASDLELYDVTENSMRVRWDAVPGATGYLILYAPLTEGLAGDEKEMKIGETHTDIELSGLFPNTEYTVTVYAMFGEEASDPATGQETTLPLTPPRNLRISNVGSNSARLTWDPASGKISGYRIVYTSADGTEINEVEVDPITTFPLKGLTPLTEYSIAIFSIYEEGQSLPLVGEFTTEEVPAQQYLEIDEVKTDSFRVTWHPLSAEEGQHKLMWIPVYGGKTQEVDLKEEQDSYVIEGLDPGTEYEVSLLAVLDDGSESEVVTAVGTTLDDFWTEAPTAIEPTSPVTSVLQTGIRNLVVDDETATSLRVSWDISDSNVEQFRVTYLKAQGDPMEEVVMVPGVQNSLLLKALLPDTEYKVTVTPVYTVGEGVSVSAPGKTLPSSGPQNLRVSEEWYNRVRITWDPPSGPVKGYRIVYKPVSVPGQTLETFVGADINTIVMTNLLSGMDYNVKIFASQASGFSDALTGLVQTLFLGVTDLQANQVEMTSLCARWQIHRHATAYRIVLESLQDTQAQESTVGGGVNRHCFYGLQPDSEYKISVYTKLQELEGPSVSIMQKTQSLPTEPPTFPPTIPPAKEVCKAAKADLVFMVDGSWSIGDDNFNKIINFLYSTVGALDKIGADGTQVAMVQFTDDPRTEFKLDSYKTKETLLDAIRHISYKGGNTKTGKAIKHVRDTLFTSDSGTRRGIPKVIVVITDGRSQDDVNKISREMQADGFNIFAIGVADADYSELVQIGSKPSSRHVFFVDDFDAFKKIEDELITFVCETASATCPMVHKDGVDLAGFKMMEMFGLVEKDFSAVEGVSMEPGTFNLFPCYQIHKDALVSQPTKYLHPEGLPSDYTMSFLFRILPDTPQEPFALWEILNKNSEPLVGIILDNGGKTLTYFNYDYTGDFQTVTFEGPDIRKMFYGSFHKLHVVVSKTLAKVVVDCKEVGQKAINASANITSDGVEVLGRMVRSRGPNGNSAPFQLQMFDIVCSTSWASKDRCCELPGLRDEESCPDLPRSCSCSETNEVALGPAGPPGGPGLRGPKGQQGEQGPKGPEGPRGETGPAGPQGPPGPQGPSGLSIQGMPGMPGDKGDKGDAGLPGPQGVPGGVGSPGRDGSPGQRGFPGKDGSSGPPGPPGPIGIPGAPGVPGITGSMGPQGALGPPGVPGAKGERGERGDLQSQAMVRAVARQVCEQLIQSHMARYTAILNQIPSQSSSIRTIQGPPGEPGRPGSPGTPGEQGPPGTPGFPGNAGVPGTPGERGLTGVKGEKGNPGIGTQGPRGPPGPAGPSGESRPGSPGPPGSPGPRGPPGHLGVPGPQGPSGQPGYCDPSSCSAYGVGVSHPDQPEFTPVQDEQEAMDLWSAGI

Target ORF information:

RefSeq Version	NM_181277.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XIV, alpha 1 (Col14a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181277.3

ORF Insert Sequence:

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTTATGG TGCCTGGAGT GCAAAACAGT CTCCTTCTGA AGGCTCTACT TCCAGACACT 
GAGTATAAAG TCACAGTAAC CCCTGTCTAT ACTGTTGGAG AAGGCGTCAG CGTCTCCGCT 
CCAGGGAAGA CTTTGCCCTC CTCTGGTCCT CAAAACCTGA GGGTCTCAGA AGAATGGTAC 
AACCGGGTGC GCATTACATG GGACCCACCC TCTGGCCCCG TGAAGGGCTA TAGGATTGTC 
TACAAACCTG TGAGTGTCCC TGGTCAAACT CTGGAGACCT TCGTGGGTGC TGACATCAAC 
ACCATTGTCA TGACAAATCT CCTCAGTGGA ATGGACTACA ATGTGAAGAT CTTTGCTTCC 
CAGGCGTCAG GCTTCAGTGA TGCTCTGACT GGCCTCGTGC AAACACTGTT CTTAGGGGTG 
ACAGATCTCC AAGCCAATCA GGTTGAAATG ACAAGCTTGT GTGCTCGGTG GCAAATACAT 
CGCCATGCCA CAGCCTACAG GATAGTTCTA GAATCCCTTC AGGACACACA AGCACAGGAA 
TCCACGGTGG GAGGAGGCGT AAACAGGCAT TGCTTCTATG GACTTCAGCC TGATTCAGAG 
TATAAAATCA GTGTTTACAC AAAGCTCCAG GAGCTAGAGG GACCCAGTGT GAGCATCATG 
CAGAAAACAC AGTCACTTCC CACGGAACCA CCGACTTTTC CTCCAACCAT CCCACCAGCA 
AAGGAAGTAT GTAAAGCTGC CAAGGCAGAC CTGGTATTCA TGGTGGATGG ATCTTGGAGC 
ATAGGAGATG ACAATTTCAA TAAAATCATC AATTTTCTAT ACAGCACTGT GGGAGCCCTG 
GACAAGATTG GTGCAGATGG AACTCAGGTG GCAATGGTTC AGTTCACTGA TGACCCCCGA 
ACAGAATTCA AACTTGATTC TTACAAAACC AAAGAGACCC TTCTTGATGC AATTAGACAC 
ATTTCCTACA AAGGAGGAAA TACTAAAACA GGAAAAGCTA TCAAGCATGT TCGGGATACC 
TTATTTACAT CAGACTCAGG GACAAGGAGA GGCATCCCAA AGGTCATCGT GGTGATAACT 
GATGGACGGT CACAAGATGA TGTCAACAAA ATCTCCAGGG AGATGCAGGC AGATGGTTTC 
AACATCTTTG CCATCGGTGT GGCTGACGCT GACTACTCCG AGTTGGTTCA GATCGGCAGC 
AAGCCCAGCT CACGGCATGT CTTCTTTGTG GATGACTTTG ATGCCTTTAA GAAAATTGAA 
GATGAATTGA TTACTTTTGT CTGTGAAACT GCATCAGCAA CCTGCCCAAT GGTGCACAAG 
GATGGAGTTG ATCTTGCAGG GTTTAAGATG ATGGAAATGT TTGGTTTGGT TGAAAAGGAC 
TTTTCTGCAG TGGAAGGAGT ATCTATGGAA CCTGGTACCT TCAATTTGTT CCCATGCTAT 
CAAATCCATA AGGATGCTCT GGTTTCTCAG CCAACAAAGT ATCTGCACCC AGAAGGATTG 
CCTTCTGACT ATACAATGAG TTTCCTGTTC CGGATTCTTC CTGACACACC ACAGGAGCCA 
TTTGCTCTTT GGGAGATTTT AAACAAAAAT TCTGAGCCGC TAGTTGGGAT CATATTGGAC 
AATGGTGGGA AAACTCTAAC TTATTTCAAC TATGACTATA CTGGAGATTT TCAAACTGTC 
ACTTTTGAAG GACCCGACAT CAGGAAAATG TTCTATGGAA GCTTTCACAA GCTCCATGTT 
GTTGTCAGCA AGACTCTGGC CAAAGTGGTT GTTGACTGCA AGGAAGTTGG TCAGAAGGCA 
ATAAATGCAT CAGCGAATAT CACATCGGAT GGTGTGGAAG TACTAGGAAG AATGGTCAGA 
TCAAGAGGAC CAAATGGAAA CTCTGCACCA TTCCAGTTAC AAATGTTTGA TATCGTTTGC 
TCTACATCAT GGGCCAGTAA AGACAGATGC TGCGAACTTC CTGGTCTGCG GGATGAAGAG 
TCCTGCCCAG ACCTTCCACG GTCCTGCTCT TGCTCGGAAA CCAATGAAGT GGCTCTAGGA 
CCAGCTGGCC CCCCAGGTGG TCCAGGACTC CGAGGACCAA AAGGCCAACA AGGTGAACAG 
GGTCCGAAGG GACCAGAGGG ACCTCGGGGA GAAACAGGTC CTGCAGGACC CCAGGGCCCC 
CCTGGACCCC AGGGACCTAG TGGTCTGTCC ATTCAAGGCA TGCCTGGAAT GCCGGGTGAT 
AAAGGAGATA AAGGAGATGC TGGTCTTCCT GGTCCACAGG GTGTCCCTGG AGGTGTTGGT 
TCACCAGGCC GTGATGGCTC ACCAGGCCAG AGGGGATTTC CCGGGAAGGA CGGTTCCTCT 
GGGCCTCCAG GACCACCTGG GCCAATTGGC ATTCCTGGAG CCCCTGGTGT CCCAGGGATC 
ACAGGCAGCA TGGGACCGCA AGGAGCCCTG GGGCCACCTG GTGTCCCTGG AGCGAAGGGA 
GAGCGAGGTG AACGGGGAGA CCTGCAGTCT CAAGCCATGG TGAGGGCGGT GGCACGTCAA 
GTATGCGAGC AGCTCATTCA GAGTCACATG GCCAGGTACA CAGCCATCCT CAACCAGATC 
CCCAGCCAGT CCTCATCTAT CCGGACCATC CAGGGCCCTC CTGGGGAGCC CGGGAGACCA 
GGTTCACCTG GAACCCCTGG AGAACAAGGA CCCCCAGGTA CCCCAGGCTT CCCAGGAAAT 
GCAGGAGTTC CGGGGACCCC AGGAGAACGA GGTCTAACTG GTGTCAAAGG AGAGAAAGGA 
AATCCAGGCA TTGGAACTCA AGGCCCAAGA GGTCCCCCTG GGCCAGCAGG ACCTTCAGGG 
GAAAGTCGTC CTGGAAGTCC TGGGCCACCT GGCTCTCCTG GACCAAGGGG TCCCCCGGGC 
CACCTGGGAG TACCTGGACC TCAGGGTCCT TCTGGCCAGC CTGGGTATTG TGACCCCTCC 
TCGTGCTCTG CTTATGGCGT TGGAGTGTCC CATCCTGATC AGCCTGAGTT CACACCGGTC 
CAGGACGAGC AGGAGGCCAT GGATCTGTGG AGCGCAGGCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col14a1 ( NM_181277.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_851794.3 Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant 2, mRNA.

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CloneID	OMu07946
Clone ID Related Accession (Same CDS sequence)	NM_181277.3 , NM_181277.4
Accession Version	NM_181277.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XIV) chain isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131337.16 and AC133156.7. On Feb 13, 2019 this sequence version replaced NM_181277.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138345.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTTATGG TGCCTGGAGT GCAAAACAGT CTCCTTCTGA AGGCTCTACT TCCAGACACT 
GAGTATAAAG TCACAGTAAC CCCTGTCTAT ACTGTTGGAG AAGGCGTCAG CGTCTCCGCT 
CCAGGGAAGA CTTTGCCCTC CTCTGGTCCT CAAAACCTGA GGGTCTCAGA AGAATGGTAC 
AACCGGGTGC GCATTACATG GGACCCACCC TCTGGCCCCG TGAAGGGCTA TAGGATTGTC 
TACAAACCTG TGAGTGTCCC TGGTCAAACT CTGGAGACCT TCGTGGGTGC TGACATCAAC 
ACCATTGTCA TGACAAATCT CCTCAGTGGA ATGGACTACA ATGTGAAGAT CTTTGCTTCC 
CAGGCGTCAG GCTTCAGTGA TGCTCTGACT GGCCTCGTGC AAACACTGTT CTTAGGGGTG 
ACAGATCTCC AAGCCAATCA GGTTGAAATG ACAAGCTTGT GTGCTCGGTG GCAAATACAT 
CGCCATGCCA CAGCCTACAG GATAGTTCTA GAATCCCTTC AGGACACACA AGCACAGGAA 
TCCACGGTGG GAGGAGGCGT AAACAGGCAT TGCTTCTATG GACTTCAGCC TGATTCAGAG 
TATAAAATCA GTGTTTACAC AAAGCTCCAG GAGCTAGAGG GACCCAGTGT GAGCATCATG 
CAGAAAACAC AGTCACTTCC CACGGAACCA CCGACTTTTC CTCCAACCAT CCCACCAGCA 
AAGGAAGTAT GTAAAGCTGC CAAGGCAGAC CTGGTATTCA TGGTGGATGG ATCTTGGAGC 
ATAGGAGATG ACAATTTCAA TAAAATCATC AATTTTCTAT ACAGCACTGT GGGAGCCCTG 
GACAAGATTG GTGCAGATGG AACTCAGGTG GCAATGGTTC AGTTCACTGA TGACCCCCGA 
ACAGAATTCA AACTTGATTC TTACAAAACC AAAGAGACCC TTCTTGATGC AATTAGACAC 
ATTTCCTACA AAGGAGGAAA TACTAAAACA GGAAAAGCTA TCAAGCATGT TCGGGATACC 
TTATTTACAT CAGACTCAGG GACAAGGAGA GGCATCCCAA AGGTCATCGT GGTGATAACT 
GATGGACGGT CACAAGATGA TGTCAACAAA ATCTCCAGGG AGATGCAGGC AGATGGTTTC 
AACATCTTTG CCATCGGTGT GGCTGACGCT GACTACTCCG AGTTGGTTCA GATCGGCAGC 
AAGCCCAGCT CACGGCATGT CTTCTTTGTG GATGACTTTG ATGCCTTTAA GAAAATTGAA 
GATGAATTGA TTACTTTTGT CTGTGAAACT GCATCAGCAA CCTGCCCAAT GGTGCACAAG 
GATGGAGTTG ATCTTGCAGG GTTTAAGATG ATGGAAATGT TTGGTTTGGT TGAAAAGGAC 
TTTTCTGCAG TGGAAGGAGT ATCTATGGAA CCTGGTACCT TCAATTTGTT CCCATGCTAT 
CAAATCCATA AGGATGCTCT GGTTTCTCAG CCAACAAAGT ATCTGCACCC AGAAGGATTG 
CCTTCTGACT ATACAATGAG TTTCCTGTTC CGGATTCTTC CTGACACACC ACAGGAGCCA 
TTTGCTCTTT GGGAGATTTT AAACAAAAAT TCTGAGCCGC TAGTTGGGAT CATATTGGAC 
AATGGTGGGA AAACTCTAAC TTATTTCAAC TATGACTATA CTGGAGATTT TCAAACTGTC 
ACTTTTGAAG GACCCGACAT CAGGAAAATG TTCTATGGAA GCTTTCACAA GCTCCATGTT 
GTTGTCAGCA AGACTCTGGC CAAAGTGGTT GTTGACTGCA AGGAAGTTGG TCAGAAGGCA 
ATAAATGCAT CAGCGAATAT CACATCGGAT GGTGTGGAAG TACTAGGAAG AATGGTCAGA 
TCAAGAGGAC CAAATGGAAA CTCTGCACCA TTCCAGTTAC AAATGTTTGA TATCGTTTGC 
TCTACATCAT GGGCCAGTAA AGACAGATGC TGCGAACTTC CTGGTCTGCG GGATGAAGAG 
TCCTGCCCAG ACCTTCCACG GTCCTGCTCT TGCTCGGAAA CCAATGAAGT GGCTCTAGGA 
CCAGCTGGCC CCCCAGGTGG TCCAGGACTC CGAGGACCAA AAGGCCAACA AGGTGAACAG 
GGTCCGAAGG GACCAGAGGG ACCTCGGGGA GAAACAGGTC CTGCAGGACC CCAGGGCCCC 
CCTGGACCCC AGGGACCTAG TGGTCTGTCC ATTCAAGGCA TGCCTGGAAT GCCGGGTGAT 
AAAGGAGATA AAGGAGATGC TGGTCTTCCT GGTCCACAGG GTGTCCCTGG AGGTGTTGGT 
TCACCAGGCC GTGATGGCTC ACCAGGCCAG AGGGGATTTC CCGGGAAGGA CGGTTCCTCT 
GGGCCTCCAG GACCACCTGG GCCAATTGGC ATTCCTGGAG CCCCTGGTGT CCCAGGGATC 
ACAGGCAGCA TGGGACCGCA AGGAGCCCTG GGGCCACCTG GTGTCCCTGG AGCGAAGGGA 
GAGCGAGGTG AACGGGGAGA CCTGCAGTCT CAAGCCATGG TGAGGGCGGT GGCACGTCAA 
GTATGCGAGC AGCTCATTCA GAGTCACATG GCCAGGTACA CAGCCATCCT CAACCAGATC 
CCCAGCCAGT CCTCATCTAT CCGGACCATC CAGGGCCCTC CTGGGGAGCC CGGGAGACCA 
GGTTCACCTG GAACCCCTGG AGAACAAGGA CCCCCAGGTA CCCCAGGCTT CCCAGGAAAT 
GCAGGAGTTC CGGGGACCCC AGGAGAACGA GGTCTAACTG GTGTCAAAGG AGAGAAAGGA 
AATCCAGGCA TTGGAACTCA AGGCCCAAGA GGTCCCCCTG GGCCAGCAGG ACCTTCAGGG 
GAAAGTCGTC CTGGAAGTCC TGGGCCACCT GGCTCTCCTG GACCAAGGGG TCCCCCGGGC 
CACCTGGGAG TACCTGGACC TCAGGGTCCT TCTGGCCAGC CTGGGTATTG TGACCCCTCC 
TCGTGCTCTG CTTATGGCGT TGGAGTGTCC CATCCTGATC AGCCTGAGTT CACACCGGTC 
CAGGACGAGC AGGAGGCCAT GGATCTGTGG AGCGCAGGCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851794.3
CDS	272..5656
Translation	MMIWQCKMRDWLILAFLAAACFCTIVRGQVAPPTRLRYNVISHDSIQISWKAPRGKFGGYKLLVAPASGGKTNQMNLQNTATKAIIQGLLPEQNYTVQLIAYYKDKESKPAQGQFRIKDLEKRKDPTKPKVKVVDKGNGSKPTSPEEVKFFCETPAIADIVILVDGSWSIGRFNFRLVRNFLENLVTAFNVGSEKTRIGLAQYSGDPRIEWHLNAFNTKDEVIDAVRSLPYKGGNTLTGLALNFIFENSFKPEAGSRSGVSKIGILITDGKSQDDIIPPSRNLRESGVELFAIGVKNADLSELQEIASEPDSTHVYNVAEFDLMHTVVESLTRTVCSRVEEQDKEIKASALATIGPPTELITSEVTARSFMVNWTQSPGKVEKYRVVYYPTRGGKPEEVVVDGSVSSTVLKNLMSSTEYQIAVFAVSAHTASEGLRGAETTLALPMASDLELYDVTENSMRVRWDAVPGATGYLILYAPLTEGLAGDEKEMKIGETHTDIELSGLFPNTEYTVTVYAMFGEEASDPATGQETTLPLTPPRNLRISNVGSNSARLTWDPASGKISGYRIVYTSADGTEINEVEVDPITTFPLKGLTPLTEYSIAIFSIYEEGQSLPLVGEFTTEEVPAQQYLEIDEVKTDSFRVTWHPLSAEEGQHKLMWIPVYGGKTQEVDLKEEQDSYVIEGLDPGTEYEVSLLAVLDDGSESEVVTAVGTTLDDFWTEAPTAIEPTSPVTSVLQTGIRNLVVDDETATSLRVSWDISDSNVEQFRVTYLKAQGDPMEEVVMVPGVQNSLLLKALLPDTEYKVTVTPVYTVGEGVSVSAPGKTLPSSGPQNLRVSEEWYNRVRITWDPPSGPVKGYRIVYKPVSVPGQTLETFVGADINTIVMTNLLSGMDYNVKIFASQASGFSDALTGLVQTLFLGVTDLQANQVEMTSLCARWQIHRHATAYRIVLESLQDTQAQESTVGGGVNRHCFYGLQPDSEYKISVYTKLQELEGPSVSIMQKTQSLPTEPPTFPPTIPPAKEVCKAAKADLVFMVDGSWSIGDDNFNKIINFLYSTVGALDKIGADGTQVAMVQFTDDPRTEFKLDSYKTKETLLDAIRHISYKGGNTKTGKAIKHVRDTLFTSDSGTRRGIPKVIVVITDGRSQDDVNKISREMQADGFNIFAIGVADADYSELVQIGSKPSSRHVFFVDDFDAFKKIEDELITFVCETASATCPMVHKDGVDLAGFKMMEMFGLVEKDFSAVEGVSMEPGTFNLFPCYQIHKDALVSQPTKYLHPEGLPSDYTMSFLFRILPDTPQEPFALWEILNKNSEPLVGIILDNGGKTLTYFNYDYTGDFQTVTFEGPDIRKMFYGSFHKLHVVVSKTLAKVVVDCKEVGQKAINASANITSDGVEVLGRMVRSRGPNGNSAPFQLQMFDIVCSTSWASKDRCCELPGLRDEESCPDLPRSCSCSETNEVALGPAGPPGGPGLRGPKGQQGEQGPKGPEGPRGETGPAGPQGPPGPQGPSGLSIQGMPGMPGDKGDKGDAGLPGPQGVPGGVGSPGRDGSPGQRGFPGKDGSSGPPGPPGPIGIPGAPGVPGITGSMGPQGALGPPGVPGAKGERGERGDLQSQAMVRAVARQVCEQLIQSHMARYTAILNQIPSQSSSIRTIQGPPGEPGRPGSPGTPGEQGPPGTPGFPGNAGVPGTPGERGLTGVKGEKGNPGIGTQGPRGPPGPAGPSGESRPGSPGPPGSPGPRGPPGHLGVPGPQGPSGQPGYCDPSSCSAYGVGVSHPDQPEFTPVQDEQEAMDLWSAGI

Target ORF information:

RefSeq Version	NM_181277.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181277.4

ORF Insert Sequence:

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTTATGG TGCCTGGAGT GCAAAACAGT CTCCTTCTGA AGGCTCTACT TCCAGACACT 
GAGTATAAAG TCACAGTAAC CCCTGTCTAT ACTGTTGGAG AAGGCGTCAG CGTCTCCGCT 
CCAGGGAAGA CTTTGCCCTC CTCTGGTCCT CAAAACCTGA GGGTCTCAGA AGAATGGTAC 
AACCGGGTGC GCATTACATG GGACCCACCC TCTGGCCCCG TGAAGGGCTA TAGGATTGTC 
TACAAACCTG TGAGTGTCCC TGGTCAAACT CTGGAGACCT TCGTGGGTGC TGACATCAAC 
ACCATTGTCA TGACAAATCT CCTCAGTGGA ATGGACTACA ATGTGAAGAT CTTTGCTTCC 
CAGGCGTCAG GCTTCAGTGA TGCTCTGACT GGCCTCGTGC AAACACTGTT CTTAGGGGTG 
ACAGATCTCC AAGCCAATCA GGTTGAAATG ACAAGCTTGT GTGCTCGGTG GCAAATACAT 
CGCCATGCCA CAGCCTACAG GATAGTTCTA GAATCCCTTC AGGACACACA AGCACAGGAA 
TCCACGGTGG GAGGAGGCGT AAACAGGCAT TGCTTCTATG GACTTCAGCC TGATTCAGAG 
TATAAAATCA GTGTTTACAC AAAGCTCCAG GAGCTAGAGG GACCCAGTGT GAGCATCATG 
CAGAAAACAC AGTCACTTCC CACGGAACCA CCGACTTTTC CTCCAACCAT CCCACCAGCA 
AAGGAAGTAT GTAAAGCTGC CAAGGCAGAC CTGGTATTCA TGGTGGATGG ATCTTGGAGC 
ATAGGAGATG ACAATTTCAA TAAAATCATC AATTTTCTAT ACAGCACTGT GGGAGCCCTG 
GACAAGATTG GTGCAGATGG AACTCAGGTG GCAATGGTTC AGTTCACTGA TGACCCCCGA 
ACAGAATTCA AACTTGATTC TTACAAAACC AAAGAGACCC TTCTTGATGC AATTAGACAC 
ATTTCCTACA AAGGAGGAAA TACTAAAACA GGAAAAGCTA TCAAGCATGT TCGGGATACC 
TTATTTACAT CAGACTCAGG GACAAGGAGA GGCATCCCAA AGGTCATCGT GGTGATAACT 
GATGGACGGT CACAAGATGA TGTCAACAAA ATCTCCAGGG AGATGCAGGC AGATGGTTTC 
AACATCTTTG CCATCGGTGT GGCTGACGCT GACTACTCCG AGTTGGTTCA GATCGGCAGC 
AAGCCCAGCT CACGGCATGT CTTCTTTGTG GATGACTTTG ATGCCTTTAA GAAAATTGAA 
GATGAATTGA TTACTTTTGT CTGTGAAACT GCATCAGCAA CCTGCCCAAT GGTGCACAAG 
GATGGAGTTG ATCTTGCAGG GTTTAAGATG ATGGAAATGT TTGGTTTGGT TGAAAAGGAC 
TTTTCTGCAG TGGAAGGAGT ATCTATGGAA CCTGGTACCT TCAATTTGTT CCCATGCTAT 
CAAATCCATA AGGATGCTCT GGTTTCTCAG CCAACAAAGT ATCTGCACCC AGAAGGATTG 
CCTTCTGACT ATACAATGAG TTTCCTGTTC CGGATTCTTC CTGACACACC ACAGGAGCCA 
TTTGCTCTTT GGGAGATTTT AAACAAAAAT TCTGAGCCGC TAGTTGGGAT CATATTGGAC 
AATGGTGGGA AAACTCTAAC TTATTTCAAC TATGACTATA CTGGAGATTT TCAAACTGTC 
ACTTTTGAAG GACCCGACAT CAGGAAAATG TTCTATGGAA GCTTTCACAA GCTCCATGTT 
GTTGTCAGCA AGACTCTGGC CAAAGTGGTT GTTGACTGCA AGGAAGTTGG TCAGAAGGCA 
ATAAATGCAT CAGCGAATAT CACATCGGAT GGTGTGGAAG TACTAGGAAG AATGGTCAGA 
TCAAGAGGAC CAAATGGAAA CTCTGCACCA TTCCAGTTAC AAATGTTTGA TATCGTTTGC 
TCTACATCAT GGGCCAGTAA AGACAGATGC TGCGAACTTC CTGGTCTGCG GGATGAAGAG 
TCCTGCCCAG ACCTTCCACG GTCCTGCTCT TGCTCGGAAA CCAATGAAGT GGCTCTAGGA 
CCAGCTGGCC CCCCAGGTGG TCCAGGACTC CGAGGACCAA AAGGCCAACA AGGTGAACAG 
GGTCCGAAGG GACCAGAGGG ACCTCGGGGA GAAACAGGTC CTGCAGGACC CCAGGGCCCC 
CCTGGACCCC AGGGACCTAG TGGTCTGTCC ATTCAAGGCA TGCCTGGAAT GCCGGGTGAT 
AAAGGAGATA AAGGAGATGC TGGTCTTCCT GGTCCACAGG GTGTCCCTGG AGGTGTTGGT 
TCACCAGGCC GTGATGGCTC ACCAGGCCAG AGGGGATTTC CCGGGAAGGA CGGTTCCTCT 
GGGCCTCCAG GACCACCTGG GCCAATTGGC ATTCCTGGAG CCCCTGGTGT CCCAGGGATC 
ACAGGCAGCA TGGGACCGCA AGGAGCCCTG GGGCCACCTG GTGTCCCTGG AGCGAAGGGA 
GAGCGAGGTG AACGGGGAGA CCTGCAGTCT CAAGCCATGG TGAGGGCGGT GGCACGTCAA 
GTATGCGAGC AGCTCATTCA GAGTCACATG GCCAGGTACA CAGCCATCCT CAACCAGATC 
CCCAGCCAGT CCTCATCTAT CCGGACCATC CAGGGCCCTC CTGGGGAGCC CGGGAGACCA 
GGTTCACCTG GAACCCCTGG AGAACAAGGA CCCCCAGGTA CCCCAGGCTT CCCAGGAAAT 
GCAGGAGTTC CGGGGACCCC AGGAGAACGA GGTCTAACTG GTGTCAAAGG AGAGAAAGGA 
AATCCAGGCA TTGGAACTCA AGGCCCAAGA GGTCCCCCTG GGCCAGCAGG ACCTTCAGGG 
GAAAGTCGTC CTGGAAGTCC TGGGCCACCT GGCTCTCCTG GACCAAGGGG TCCCCCGGGC 
CACCTGGGAG TACCTGGACC TCAGGGTCCT TCTGGCCAGC CTGGGTATTG TGACCCCTCC 
TCGTGCTCTG CTTATGGCGT TGGAGTGTCC CATCCTGATC AGCCTGAGTT CACACCGGTC 
CAGGACGAGC AGGAGGCCAT GGATCTGTGG AGCGCAGGCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col14a1 ( NM_001368422.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001355351.1 Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant 1, mRNA.

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CloneID	OMu50906
Clone ID Related Accession (Same CDS sequence)	NM_001368422.1 , XM_006520384.3
Accession Version	NM_001368422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XIV) chain isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131337.16 and AC133156.7. On Feb 13, 2019 this sequence version replaced XM_006520384.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTAGGAA CGGTTATGGT GCCTGGAGTG CAAAACAGTC TCCTTCTGAA GGCTCTACTT 
CCAGACACTG AGTATAAAGT CACAGTAACC CCTGTCTATA CTGTTGGAGA AGGCGTCAGC 
GTCTCCGCTC CAGGGAAGAC TTTGCCCTCC TCTGGTCCTC AAAACCTGAG GGTCTCAGAA 
GAATGGTACA ACCGGGTGCG CATTACATGG GACCCACCCT CTGGCCCCGT GAAGGGCTAT 
AGGATTGTCT ACAAACCTGT GAGTGTCCCT GGTCAAACTC TGGAGACCTT CGTGGGTGCT 
GACATCAACA CCATTGTCAT GACAAATCTC CTCAGTGGAA TGGACTACAA TGTGAAGATC 
TTTGCTTCCC AGGCGTCAGG CTTCAGTGAT GCTCTGACTG GCCTCGTGCA AACACTGTTC 
TTAGGGGTGA CAGATCTCCA AGCCAATCAG GTTGAAATGA CAAGCTTGTG TGCTCGGTGG 
CAAATACATC GCCATGCCAC AGCCTACAGG ATAGTTCTAG AATCCCTTCA GGACACACAA 
GCACAGGAAT CCACGGTGGG AGGAGGCGTA AACAGGCATT GCTTCTATGG ACTTCAGCCT 
GATTCAGAGT ATAAAATCAG TGTTTACACA AAGCTCCAGG AGCTAGAGGG ACCCAGTGTG 
AGCATCATGC AGAAAACACA GTCACTTCCC ACGGAACCAC CGACTTTTCC TCCAACCATC 
CCACCAGCAA AGGAAGTATG TAAAGCTGCC AAGGCAGACC TGGTATTCAT GGTGGATGGA 
TCTTGGAGCA TAGGAGATGA CAATTTCAAT AAAATCATCA ATTTTCTATA CAGCACTGTG 
GGAGCCCTGG ACAAGATTGG TGCAGATGGA ACTCAGGTGG CAATGGTTCA GTTCACTGAT 
GACCCCCGAA CAGAATTCAA ACTTGATTCT TACAAAACCA AAGAGACCCT TCTTGATGCA 
ATTAGACACA TTTCCTACAA AGGAGGAAAT ACTAAAACAG GAAAAGCTAT CAAGCATGTT 
CGGGATACCT TATTTACATC AGACTCAGGG ACAAGGAGAG GCATCCCAAA GGTCATCGTG 
GTGATAACTG ATGGACGGTC ACAAGATGAT GTCAACAAAA TCTCCAGGGA GATGCAGGCA 
GATGGTTTCA ACATCTTTGC CATCGGTGTG GCTGACGCTG ACTACTCCGA GTTGGTTCAG 
ATCGGCAGCA AGCCCAGCTC ACGGCATGTC TTCTTTGTGG ATGACTTTGA TGCCTTTAAG 
AAAATTGAAG ATGAATTGAT TACTTTTGTC TGTGAAACTG CATCAGCAAC CTGCCCAATG 
GTGCACAAGG ATGGAGTTGA TCTTGCAGGG TTTAAGATGA TGGAAATGTT TGGTTTGGTT 
GAAAAGGACT TTTCTGCAGT GGAAGGAGTA TCTATGGAAC CTGGTACCTT CAATTTGTTC 
CCATGCTATC AAATCCATAA GGATGCTCTG GTTTCTCAGC CAACAAAGTA TCTGCACCCA 
GAAGGATTGC CTTCTGACTA TACAATGAGT TTCCTGTTCC GGATTCTTCC TGACACACCA 
CAGGAGCCAT TTGCTCTTTG GGAGATTTTA AACAAAAATT CTGAGCCGCT AGTTGGGATC 
ATATTGGACA ATGGTGGGAA AACTCTAACT TATTTCAACT ATGACTATAC TGGAGATTTT 
CAAACTGTCA CTTTTGAAGG ACCCGACATC AGGAAAATGT TCTATGGAAG CTTTCACAAG 
CTCCATGTTG TTGTCAGCAA GACTCTGGCC AAAGTGGTTG TTGACTGCAA GGAAGTTGGT 
CAGAAGGCAA TAAATGCATC AGCGAATATC ACATCGGATG GTGTGGAAGT ACTAGGAAGA 
ATGGTCAGAT CAAGAGGACC AAATGGAAAC TCTGCACCAT TCCAGTTACA AATGTTTGAT 
ATCGTTTGCT CTACATCATG GGCCAGTAAA GACAGATGCT GCGAACTTCC TGGTCTGCGG 
GATGAAGAGT CCTGCCCAGA CCTTCCACGG TCCTGCTCTT GCTCGGAAAC CAATGAAGTG 
GCTCTAGGAC CAGCTGGCCC CCCAGGTGGT CCAGGACTCC GAGGACCAAA AGGCCAACAA 
GGTGAACAGG GTCCGAAGGG ACCAGAGGGA CCTCGGGGAG AAACAGGTCC TGCAGGACCC 
CAGGGCCCCC CTGGACCCCA GGGACCTAGT GGTCTGTCCA TTCAAGGCAT GCCTGGAATG 
CCGGGTGATA AAGGAGATAA AGGAGATGCT GGTCTTCCTG GTCCACAGGG TGTCCCTGGA 
GGTGTTGGTT CACCAGGCCG TGATGGCTCA CCAGGCCAGA GGGGATTTCC CGGGAAGGAC 
GGTTCCTCTG GGCCTCCAGG ACCACCTGGG CCAATTGGCA TTCCTGGAGC CCCTGGTGTC 
CCAGGGATCA CAGGCAGCAT GGGACCGCAA GGAGCCCTGG GGCCACCTGG TGTCCCTGGA 
GCGAAGGGAG AGCGAGGTGA ACGGGGAGAC CTGCAGTCTC AAGCCATGGT GAGGGCGGTG 
GCACGTCAAG TATGCGAGCA GCTCATTCAG AGTCACATGG CCAGGTACAC AGCCATCCTC 
AACCAGATCC CCAGCCAGTC CTCATCTATC CGGACCATCC AGGGCCCTCC TGGGGAGCCC 
GGGAGACCAG GTTCACCTGG AACCCCTGGA GAACAAGGAC CCCCAGGTAC CCCAGGCTTC 
CCAGGAAATG CAGGAGTTCC GGGGACCCCA GGAGAACGAG GTCTAACTGG TGTCAAAGGA 
GAGAAAGGAA ATCCAGGCAT TGGAACTCAA GGCCCAAGAG GTCCCCCTGG GCCAGCAGGA 
CCTTCAGGGG AAAGTCGTCC TGGAAGTCCT GGGCCACCTG GCTCTCCTGG ACCAAGGGGT 
CCCCCGGGCC ACCTGGGAGT ACCTGGACCT CAGGGTCCTT CTGGCCAGCC TGGGTATTGT 
GACCCCTCCT CGTGCTCTGC TTATGGCGTT GGAGTGTCCC ATCCTGATCA GCCTGAGTTC 
ACACCGGTCC AGGACGAGCA GGAGGCCATG GATCTGTGGA GCGCAGGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355351.1
CDS	272..5665
Translation	MMIWQCKMRDWLILAFLAAACFCTIVRGQVAPPTRLRYNVISHDSIQISWKAPRGKFGGYKLLVAPASGGKTNQMNLQNTATKAIIQGLLPEQNYTVQLIAYYKDKESKPAQGQFRIKDLEKRKDPTKPKVKVVDKGNGSKPTSPEEVKFFCETPAIADIVILVDGSWSIGRFNFRLVRNFLENLVTAFNVGSEKTRIGLAQYSGDPRIEWHLNAFNTKDEVIDAVRSLPYKGGNTLTGLALNFIFENSFKPEAGSRSGVSKIGILITDGKSQDDIIPPSRNLRESGVELFAIGVKNADLSELQEIASEPDSTHVYNVAEFDLMHTVVESLTRTVCSRVEEQDKEIKASALATIGPPTELITSEVTARSFMVNWTQSPGKVEKYRVVYYPTRGGKPEEVVVDGSVSSTVLKNLMSSTEYQIAVFAVSAHTASEGLRGAETTLALPMASDLELYDVTENSMRVRWDAVPGATGYLILYAPLTEGLAGDEKEMKIGETHTDIELSGLFPNTEYTVTVYAMFGEEASDPATGQETTLPLTPPRNLRISNVGSNSARLTWDPASGKISGYRIVYTSADGTEINEVEVDPITTFPLKGLTPLTEYSIAIFSIYEEGQSLPLVGEFTTEEVPAQQYLEIDEVKTDSFRVTWHPLSAEEGQHKLMWIPVYGGKTQEVDLKEEQDSYVIEGLDPGTEYEVSLLAVLDDGSESEVVTAVGTTLDDFWTEAPTAIEPTSPVTSVLQTGIRNLVVDDETATSLRVSWDISDSNVEQFRVTYLKAQGDPMEEVVGTVMVPGVQNSLLLKALLPDTEYKVTVTPVYTVGEGVSVSAPGKTLPSSGPQNLRVSEEWYNRVRITWDPPSGPVKGYRIVYKPVSVPGQTLETFVGADINTIVMTNLLSGMDYNVKIFASQASGFSDALTGLVQTLFLGVTDLQANQVEMTSLCARWQIHRHATAYRIVLESLQDTQAQESTVGGGVNRHCFYGLQPDSEYKISVYTKLQELEGPSVSIMQKTQSLPTEPPTFPPTIPPAKEVCKAAKADLVFMVDGSWSIGDDNFNKIINFLYSTVGALDKIGADGTQVAMVQFTDDPRTEFKLDSYKTKETLLDAIRHISYKGGNTKTGKAIKHVRDTLFTSDSGTRRGIPKVIVVITDGRSQDDVNKISREMQADGFNIFAIGVADADYSELVQIGSKPSSRHVFFVDDFDAFKKIEDELITFVCETASATCPMVHKDGVDLAGFKMMEMFGLVEKDFSAVEGVSMEPGTFNLFPCYQIHKDALVSQPTKYLHPEGLPSDYTMSFLFRILPDTPQEPFALWEILNKNSEPLVGIILDNGGKTLTYFNYDYTGDFQTVTFEGPDIRKMFYGSFHKLHVVVSKTLAKVVVDCKEVGQKAINASANITSDGVEVLGRMVRSRGPNGNSAPFQLQMFDIVCSTSWASKDRCCELPGLRDEESCPDLPRSCSCSETNEVALGPAGPPGGPGLRGPKGQQGEQGPKGPEGPRGETGPAGPQGPPGPQGPSGLSIQGMPGMPGDKGDKGDAGLPGPQGVPGGVGSPGRDGSPGQRGFPGKDGSSGPPGPPGPIGIPGAPGVPGITGSMGPQGALGPPGVPGAKGERGERGDLQSQAMVRAVARQVCEQLIQSHMARYTAILNQIPSQSSSIRTIQGPPGEPGRPGSPGTPGEQGPPGTPGFPGNAGVPGTPGERGLTGVKGEKGNPGIGTQGPRGPPGPAGPSGESRPGSPGPPGSPGPRGPPGHLGVPGPQGPSGQPGYCDPSSCSAYGVGVSHPDQPEFTPVQDEQEAMDLWSAGI

Target ORF information:

RefSeq Version	NM_001368422.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368422.1

ORF Insert Sequence:

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTAGGAA CGGTTATGGT GCCTGGAGTG CAAAACAGTC TCCTTCTGAA GGCTCTACTT 
CCAGACACTG AGTATAAAGT CACAGTAACC CCTGTCTATA CTGTTGGAGA AGGCGTCAGC 
GTCTCCGCTC CAGGGAAGAC TTTGCCCTCC TCTGGTCCTC AAAACCTGAG GGTCTCAGAA 
GAATGGTACA ACCGGGTGCG CATTACATGG GACCCACCCT CTGGCCCCGT GAAGGGCTAT 
AGGATTGTCT ACAAACCTGT GAGTGTCCCT GGTCAAACTC TGGAGACCTT CGTGGGTGCT 
GACATCAACA CCATTGTCAT GACAAATCTC CTCAGTGGAA TGGACTACAA TGTGAAGATC 
TTTGCTTCCC AGGCGTCAGG CTTCAGTGAT GCTCTGACTG GCCTCGTGCA AACACTGTTC 
TTAGGGGTGA CAGATCTCCA AGCCAATCAG GTTGAAATGA CAAGCTTGTG TGCTCGGTGG 
CAAATACATC GCCATGCCAC AGCCTACAGG ATAGTTCTAG AATCCCTTCA GGACACACAA 
GCACAGGAAT CCACGGTGGG AGGAGGCGTA AACAGGCATT GCTTCTATGG ACTTCAGCCT 
GATTCAGAGT ATAAAATCAG TGTTTACACA AAGCTCCAGG AGCTAGAGGG ACCCAGTGTG 
AGCATCATGC AGAAAACACA GTCACTTCCC ACGGAACCAC CGACTTTTCC TCCAACCATC 
CCACCAGCAA AGGAAGTATG TAAAGCTGCC AAGGCAGACC TGGTATTCAT GGTGGATGGA 
TCTTGGAGCA TAGGAGATGA CAATTTCAAT AAAATCATCA ATTTTCTATA CAGCACTGTG 
GGAGCCCTGG ACAAGATTGG TGCAGATGGA ACTCAGGTGG CAATGGTTCA GTTCACTGAT 
GACCCCCGAA CAGAATTCAA ACTTGATTCT TACAAAACCA AAGAGACCCT TCTTGATGCA 
ATTAGACACA TTTCCTACAA AGGAGGAAAT ACTAAAACAG GAAAAGCTAT CAAGCATGTT 
CGGGATACCT TATTTACATC AGACTCAGGG ACAAGGAGAG GCATCCCAAA GGTCATCGTG 
GTGATAACTG ATGGACGGTC ACAAGATGAT GTCAACAAAA TCTCCAGGGA GATGCAGGCA 
GATGGTTTCA ACATCTTTGC CATCGGTGTG GCTGACGCTG ACTACTCCGA GTTGGTTCAG 
ATCGGCAGCA AGCCCAGCTC ACGGCATGTC TTCTTTGTGG ATGACTTTGA TGCCTTTAAG 
AAAATTGAAG ATGAATTGAT TACTTTTGTC TGTGAAACTG CATCAGCAAC CTGCCCAATG 
GTGCACAAGG ATGGAGTTGA TCTTGCAGGG TTTAAGATGA TGGAAATGTT TGGTTTGGTT 
GAAAAGGACT TTTCTGCAGT GGAAGGAGTA TCTATGGAAC CTGGTACCTT CAATTTGTTC 
CCATGCTATC AAATCCATAA GGATGCTCTG GTTTCTCAGC CAACAAAGTA TCTGCACCCA 
GAAGGATTGC CTTCTGACTA TACAATGAGT TTCCTGTTCC GGATTCTTCC TGACACACCA 
CAGGAGCCAT TTGCTCTTTG GGAGATTTTA AACAAAAATT CTGAGCCGCT AGTTGGGATC 
ATATTGGACA ATGGTGGGAA AACTCTAACT TATTTCAACT ATGACTATAC TGGAGATTTT 
CAAACTGTCA CTTTTGAAGG ACCCGACATC AGGAAAATGT TCTATGGAAG CTTTCACAAG 
CTCCATGTTG TTGTCAGCAA GACTCTGGCC AAAGTGGTTG TTGACTGCAA GGAAGTTGGT 
CAGAAGGCAA TAAATGCATC AGCGAATATC ACATCGGATG GTGTGGAAGT ACTAGGAAGA 
ATGGTCAGAT CAAGAGGACC AAATGGAAAC TCTGCACCAT TCCAGTTACA AATGTTTGAT 
ATCGTTTGCT CTACATCATG GGCCAGTAAA GACAGATGCT GCGAACTTCC TGGTCTGCGG 
GATGAAGAGT CCTGCCCAGA CCTTCCACGG TCCTGCTCTT GCTCGGAAAC CAATGAAGTG 
GCTCTAGGAC CAGCTGGCCC CCCAGGTGGT CCAGGACTCC GAGGACCAAA AGGCCAACAA 
GGTGAACAGG GTCCGAAGGG ACCAGAGGGA CCTCGGGGAG AAACAGGTCC TGCAGGACCC 
CAGGGCCCCC CTGGACCCCA GGGACCTAGT GGTCTGTCCA TTCAAGGCAT GCCTGGAATG 
CCGGGTGATA AAGGAGATAA AGGAGATGCT GGTCTTCCTG GTCCACAGGG TGTCCCTGGA 
GGTGTTGGTT CACCAGGCCG TGATGGCTCA CCAGGCCAGA GGGGATTTCC CGGGAAGGAC 
GGTTCCTCTG GGCCTCCAGG ACCACCTGGG CCAATTGGCA TTCCTGGAGC CCCTGGTGTC 
CCAGGGATCA CAGGCAGCAT GGGACCGCAA GGAGCCCTGG GGCCACCTGG TGTCCCTGGA 
GCGAAGGGAG AGCGAGGTGA ACGGGGAGAC CTGCAGTCTC AAGCCATGGT GAGGGCGGTG 
GCACGTCAAG TATGCGAGCA GCTCATTCAG AGTCACATGG CCAGGTACAC AGCCATCCTC 
AACCAGATCC CCAGCCAGTC CTCATCTATC CGGACCATCC AGGGCCCTCC TGGGGAGCCC 
GGGAGACCAG GTTCACCTGG AACCCCTGGA GAACAAGGAC CCCCAGGTAC CCCAGGCTTC 
CCAGGAAATG CAGGAGTTCC GGGGACCCCA GGAGAACGAG GTCTAACTGG TGTCAAAGGA 
GAGAAAGGAA ATCCAGGCAT TGGAACTCAA GGCCCAAGAG GTCCCCCTGG GCCAGCAGGA 
CCTTCAGGGG AAAGTCGTCC TGGAAGTCCT GGGCCACCTG GCTCTCCTGG ACCAAGGGGT 
CCCCCGGGCC ACCTGGGAGT ACCTGGACCT CAGGGTCCTT CTGGCCAGCC TGGGTATTGT 
GACCCCTCCT CGTGCTCTGC TTATGGCGTT GGAGTGTCCC ATCCTGATCA GCCTGAGTTC 
ACACCGGTCC AGGACGAGCA GGAGGCCATG GATCTGTGGA GCGCAGGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col14a1 ( XM_006520384.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520447.1 Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant X1, mRNA.

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CloneID	OMu50906
Clone ID Related Accession (Same CDS sequence)	NM_001368422.1 , XM_006520384.3
Accession Version	XM_006520384.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XIV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520384.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTAGGAA CGGTTATGGT GCCTGGAGTG CAAAACAGTC TCCTTCTGAA GGCTCTACTT 
CCAGACACTG AGTATAAAGT CACAGTAACC CCTGTCTATA CTGTTGGAGA AGGCGTCAGC 
GTCTCCGCTC CAGGGAAGAC TTTGCCCTCC TCTGGTCCTC AAAACCTGAG GGTCTCAGAA 
GAATGGTACA ACCGGGTGCG CATTACATGG GACCCACCCT CTGGCCCCGT GAAGGGCTAT 
AGGATTGTCT ACAAACCTGT GAGTGTCCCT GGTCAAACTC TGGAGACCTT CGTGGGTGCT 
GACATCAACA CCATTGTCAT GACAAATCTC CTCAGTGGAA TGGACTACAA TGTGAAGATC 
TTTGCTTCCC AGGCGTCAGG CTTCAGTGAT GCTCTGACTG GCCTCGTGCA AACACTGTTC 
TTAGGGGTGA CAGATCTCCA AGCCAATCAG GTTGAAATGA CAAGCTTGTG TGCTCGGTGG 
CAAATACATC GCCATGCCAC AGCCTACAGG ATAGTTCTAG AATCCCTTCA GGACACACAA 
GCACAGGAAT CCACGGTGGG AGGAGGCGTA AACAGGCATT GCTTCTATGG ACTTCAGCCT 
GATTCAGAGT ATAAAATCAG TGTTTACACA AAGCTCCAGG AGCTAGAGGG ACCCAGTGTG 
AGCATCATGC AGAAAACACA GTCACTTCCC ACGGAACCAC CGACTTTTCC TCCAACCATC 
CCACCAGCAA AGGAAGTATG TAAAGCTGCC AAGGCAGACC TGGTATTCAT GGTGGATGGA 
TCTTGGAGCA TAGGAGATGA CAATTTCAAT AAAATCATCA ATTTTCTATA CAGCACTGTG 
GGAGCCCTGG ACAAGATTGG TGCAGATGGA ACTCAGGTGG CAATGGTTCA GTTCACTGAT 
GACCCCCGAA CAGAATTCAA ACTTGATTCT TACAAAACCA AAGAGACCCT TCTTGATGCA 
ATTAGACACA TTTCCTACAA AGGAGGAAAT ACTAAAACAG GAAAAGCTAT CAAGCATGTT 
CGGGATACCT TATTTACATC AGACTCAGGG ACAAGGAGAG GCATCCCAAA GGTCATCGTG 
GTGATAACTG ATGGACGGTC ACAAGATGAT GTCAACAAAA TCTCCAGGGA GATGCAGGCA 
GATGGTTTCA ACATCTTTGC CATCGGTGTG GCTGACGCTG ACTACTCCGA GTTGGTTCAG 
ATCGGCAGCA AGCCCAGCTC ACGGCATGTC TTCTTTGTGG ATGACTTTGA TGCCTTTAAG 
AAAATTGAAG ATGAATTGAT TACTTTTGTC TGTGAAACTG CATCAGCAAC CTGCCCAATG 
GTGCACAAGG ATGGAGTTGA TCTTGCAGGG TTTAAGATGA TGGAAATGTT TGGTTTGGTT 
GAAAAGGACT TTTCTGCAGT GGAAGGAGTA TCTATGGAAC CTGGTACCTT CAATTTGTTC 
CCATGCTATC AAATCCATAA GGATGCTCTG GTTTCTCAGC CAACAAAGTA TCTGCACCCA 
GAAGGATTGC CTTCTGACTA TACAATGAGT TTCCTGTTCC GGATTCTTCC TGACACACCA 
CAGGAGCCAT TTGCTCTTTG GGAGATTTTA AACAAAAATT CTGAGCCGCT AGTTGGGATC 
ATATTGGACA ATGGTGGGAA AACTCTAACT TATTTCAACT ATGACTATAC TGGAGATTTT 
CAAACTGTCA CTTTTGAAGG ACCCGACATC AGGAAAATGT TCTATGGAAG CTTTCACAAG 
CTCCATGTTG TTGTCAGCAA GACTCTGGCC AAAGTGGTTG TTGACTGCAA GGAAGTTGGT 
CAGAAGGCAA TAAATGCATC AGCGAATATC ACATCGGATG GTGTGGAAGT ACTAGGAAGA 
ATGGTCAGAT CAAGAGGACC AAATGGAAAC TCTGCACCAT TCCAGTTACA AATGTTTGAT 
ATCGTTTGCT CTACATCATG GGCCAGTAAA GACAGATGCT GCGAACTTCC TGGTCTGCGG 
GATGAAGAGT CCTGCCCAGA CCTTCCACGG TCCTGCTCTT GCTCGGAAAC CAATGAAGTG 
GCTCTAGGAC CAGCTGGCCC CCCAGGTGGT CCAGGACTCC GAGGACCAAA AGGCCAACAA 
GGTGAACAGG GTCCGAAGGG ACCAGAGGGA CCTCGGGGAG AAACAGGTCC TGCAGGACCC 
CAGGGCCCCC CTGGACCCCA GGGACCTAGT GGTCTGTCCA TTCAAGGCAT GCCTGGAATG 
CCGGGTGATA AAGGAGATAA AGGAGATGCT GGTCTTCCTG GTCCACAGGG TGTCCCTGGA 
GGTGTTGGTT CACCAGGCCG TGATGGCTCA CCAGGCCAGA GGGGATTTCC CGGGAAGGAC 
GGTTCCTCTG GGCCTCCAGG ACCACCTGGG CCAATTGGCA TTCCTGGAGC CCCTGGTGTC 
CCAGGGATCA CAGGCAGCAT GGGACCGCAA GGAGCCCTGG GGCCACCTGG TGTCCCTGGA 
GCGAAGGGAG AGCGAGGTGA ACGGGGAGAC CTGCAGTCTC AAGCCATGGT GAGGGCGGTG 
GCACGTCAAG TATGCGAGCA GCTCATTCAG AGTCACATGG CCAGGTACAC AGCCATCCTC 
AACCAGATCC CCAGCCAGTC CTCATCTATC CGGACCATCC AGGGCCCTCC TGGGGAGCCC 
GGGAGACCAG GTTCACCTGG AACCCCTGGA GAACAAGGAC CCCCAGGTAC CCCAGGCTTC 
CCAGGAAATG CAGGAGTTCC GGGGACCCCA GGAGAACGAG GTCTAACTGG TGTCAAAGGA 
GAGAAAGGAA ATCCAGGCAT TGGAACTCAA GGCCCAAGAG GTCCCCCTGG GCCAGCAGGA 
CCTTCAGGGG AAAGTCGTCC TGGAAGTCCT GGGCCACCTG GCTCTCCTGG ACCAAGGGGT 
CCCCCGGGCC ACCTGGGAGT ACCTGGACCT CAGGGTCCTT CTGGCCAGCC TGGGTATTGT 
GACCCCTCCT CGTGCTCTGC TTATGGCGTT GGAGTGTCCC ATCCTGATCA GCCTGAGTTC 
ACACCGGTCC AGGACGAGCA GGAGGCCATG GATCTGTGGA GCGCAGGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520447.1
CDS	329..5722
Translation	MMIWQCKMRDWLILAFLAAACFCTIVRGQVAPPTRLRYNVISHDSIQISWKAPRGKFGGYKLLVAPASGGKTNQMNLQNTATKAIIQGLLPEQNYTVQLIAYYKDKESKPAQGQFRIKDLEKRKDPTKPKVKVVDKGNGSKPTSPEEVKFFCETPAIADIVILVDGSWSIGRFNFRLVRNFLENLVTAFNVGSEKTRIGLAQYSGDPRIEWHLNAFNTKDEVIDAVRSLPYKGGNTLTGLALNFIFENSFKPEAGSRSGVSKIGILITDGKSQDDIIPPSRNLRESGVELFAIGVKNADLSELQEIASEPDSTHVYNVAEFDLMHTVVESLTRTVCSRVEEQDKEIKASALATIGPPTELITSEVTARSFMVNWTQSPGKVEKYRVVYYPTRGGKPEEVVVDGSVSSTVLKNLMSSTEYQIAVFAVSAHTASEGLRGAETTLALPMASDLELYDVTENSMRVRWDAVPGATGYLILYAPLTEGLAGDEKEMKIGETHTDIELSGLFPNTEYTVTVYAMFGEEASDPATGQETTLPLTPPRNLRISNVGSNSARLTWDPASGKISGYRIVYTSADGTEINEVEVDPITTFPLKGLTPLTEYSIAIFSIYEEGQSLPLVGEFTTEEVPAQQYLEIDEVKTDSFRVTWHPLSAEEGQHKLMWIPVYGGKTQEVDLKEEQDSYVIEGLDPGTEYEVSLLAVLDDGSESEVVTAVGTTLDDFWTEAPTAIEPTSPVTSVLQTGIRNLVVDDETATSLRVSWDISDSNVEQFRVTYLKAQGDPMEEVVGTVMVPGVQNSLLLKALLPDTEYKVTVTPVYTVGEGVSVSAPGKTLPSSGPQNLRVSEEWYNRVRITWDPPSGPVKGYRIVYKPVSVPGQTLETFVGADINTIVMTNLLSGMDYNVKIFASQASGFSDALTGLVQTLFLGVTDLQANQVEMTSLCARWQIHRHATAYRIVLESLQDTQAQESTVGGGVNRHCFYGLQPDSEYKISVYTKLQELEGPSVSIMQKTQSLPTEPPTFPPTIPPAKEVCKAAKADLVFMVDGSWSIGDDNFNKIINFLYSTVGALDKIGADGTQVAMVQFTDDPRTEFKLDSYKTKETLLDAIRHISYKGGNTKTGKAIKHVRDTLFTSDSGTRRGIPKVIVVITDGRSQDDVNKISREMQADGFNIFAIGVADADYSELVQIGSKPSSRHVFFVDDFDAFKKIEDELITFVCETASATCPMVHKDGVDLAGFKMMEMFGLVEKDFSAVEGVSMEPGTFNLFPCYQIHKDALVSQPTKYLHPEGLPSDYTMSFLFRILPDTPQEPFALWEILNKNSEPLVGIILDNGGKTLTYFNYDYTGDFQTVTFEGPDIRKMFYGSFHKLHVVVSKTLAKVVVDCKEVGQKAINASANITSDGVEVLGRMVRSRGPNGNSAPFQLQMFDIVCSTSWASKDRCCELPGLRDEESCPDLPRSCSCSETNEVALGPAGPPGGPGLRGPKGQQGEQGPKGPEGPRGETGPAGPQGPPGPQGPSGLSIQGMPGMPGDKGDKGDAGLPGPQGVPGGVGSPGRDGSPGQRGFPGKDGSSGPPGPPGPIGIPGAPGVPGITGSMGPQGALGPPGVPGAKGERGERGDLQSQAMVRAVARQVCEQLIQSHMARYTAILNQIPSQSSSIRTIQGPPGEPGRPGSPGTPGEQGPPGTPGFPGNAGVPGTPGERGLTGVKGEKGNPGIGTQGPRGPPGPAGPSGESRPGSPGPPGSPGPRGPPGHLGVPGPQGPSGQPGYCDPSSCSAYGVGVSHPDQPEFTPVQDEQEAMDLWSAGI

Target ORF information:

RefSeq Version	XM_006520384.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520384.3

ORF Insert Sequence:

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTAGGAA CGGTTATGGT GCCTGGAGTG CAAAACAGTC TCCTTCTGAA GGCTCTACTT 
CCAGACACTG AGTATAAAGT CACAGTAACC CCTGTCTATA CTGTTGGAGA AGGCGTCAGC 
GTCTCCGCTC CAGGGAAGAC TTTGCCCTCC TCTGGTCCTC AAAACCTGAG GGTCTCAGAA 
GAATGGTACA ACCGGGTGCG CATTACATGG GACCCACCCT CTGGCCCCGT GAAGGGCTAT 
AGGATTGTCT ACAAACCTGT GAGTGTCCCT GGTCAAACTC TGGAGACCTT CGTGGGTGCT 
GACATCAACA CCATTGTCAT GACAAATCTC CTCAGTGGAA TGGACTACAA TGTGAAGATC 
TTTGCTTCCC AGGCGTCAGG CTTCAGTGAT GCTCTGACTG GCCTCGTGCA AACACTGTTC 
TTAGGGGTGA CAGATCTCCA AGCCAATCAG GTTGAAATGA CAAGCTTGTG TGCTCGGTGG 
CAAATACATC GCCATGCCAC AGCCTACAGG ATAGTTCTAG AATCCCTTCA GGACACACAA 
GCACAGGAAT CCACGGTGGG AGGAGGCGTA AACAGGCATT GCTTCTATGG ACTTCAGCCT 
GATTCAGAGT ATAAAATCAG TGTTTACACA AAGCTCCAGG AGCTAGAGGG ACCCAGTGTG 
AGCATCATGC AGAAAACACA GTCACTTCCC ACGGAACCAC CGACTTTTCC TCCAACCATC 
CCACCAGCAA AGGAAGTATG TAAAGCTGCC AAGGCAGACC TGGTATTCAT GGTGGATGGA 
TCTTGGAGCA TAGGAGATGA CAATTTCAAT AAAATCATCA ATTTTCTATA CAGCACTGTG 
GGAGCCCTGG ACAAGATTGG TGCAGATGGA ACTCAGGTGG CAATGGTTCA GTTCACTGAT 
GACCCCCGAA CAGAATTCAA ACTTGATTCT TACAAAACCA AAGAGACCCT TCTTGATGCA 
ATTAGACACA TTTCCTACAA AGGAGGAAAT ACTAAAACAG GAAAAGCTAT CAAGCATGTT 
CGGGATACCT TATTTACATC AGACTCAGGG ACAAGGAGAG GCATCCCAAA GGTCATCGTG 
GTGATAACTG ATGGACGGTC ACAAGATGAT GTCAACAAAA TCTCCAGGGA GATGCAGGCA 
GATGGTTTCA ACATCTTTGC CATCGGTGTG GCTGACGCTG ACTACTCCGA GTTGGTTCAG 
ATCGGCAGCA AGCCCAGCTC ACGGCATGTC TTCTTTGTGG ATGACTTTGA TGCCTTTAAG 
AAAATTGAAG ATGAATTGAT TACTTTTGTC TGTGAAACTG CATCAGCAAC CTGCCCAATG 
GTGCACAAGG ATGGAGTTGA TCTTGCAGGG TTTAAGATGA TGGAAATGTT TGGTTTGGTT 
GAAAAGGACT TTTCTGCAGT GGAAGGAGTA TCTATGGAAC CTGGTACCTT CAATTTGTTC 
CCATGCTATC AAATCCATAA GGATGCTCTG GTTTCTCAGC CAACAAAGTA TCTGCACCCA 
GAAGGATTGC CTTCTGACTA TACAATGAGT TTCCTGTTCC GGATTCTTCC TGACACACCA 
CAGGAGCCAT TTGCTCTTTG GGAGATTTTA AACAAAAATT CTGAGCCGCT AGTTGGGATC 
ATATTGGACA ATGGTGGGAA AACTCTAACT TATTTCAACT ATGACTATAC TGGAGATTTT 
CAAACTGTCA CTTTTGAAGG ACCCGACATC AGGAAAATGT TCTATGGAAG CTTTCACAAG 
CTCCATGTTG TTGTCAGCAA GACTCTGGCC AAAGTGGTTG TTGACTGCAA GGAAGTTGGT 
CAGAAGGCAA TAAATGCATC AGCGAATATC ACATCGGATG GTGTGGAAGT ACTAGGAAGA 
ATGGTCAGAT CAAGAGGACC AAATGGAAAC TCTGCACCAT TCCAGTTACA AATGTTTGAT 
ATCGTTTGCT CTACATCATG GGCCAGTAAA GACAGATGCT GCGAACTTCC TGGTCTGCGG 
GATGAAGAGT CCTGCCCAGA CCTTCCACGG TCCTGCTCTT GCTCGGAAAC CAATGAAGTG 
GCTCTAGGAC CAGCTGGCCC CCCAGGTGGT CCAGGACTCC GAGGACCAAA AGGCCAACAA 
GGTGAACAGG GTCCGAAGGG ACCAGAGGGA CCTCGGGGAG AAACAGGTCC TGCAGGACCC 
CAGGGCCCCC CTGGACCCCA GGGACCTAGT GGTCTGTCCA TTCAAGGCAT GCCTGGAATG 
CCGGGTGATA AAGGAGATAA AGGAGATGCT GGTCTTCCTG GTCCACAGGG TGTCCCTGGA 
GGTGTTGGTT CACCAGGCCG TGATGGCTCA CCAGGCCAGA GGGGATTTCC CGGGAAGGAC 
GGTTCCTCTG GGCCTCCAGG ACCACCTGGG CCAATTGGCA TTCCTGGAGC CCCTGGTGTC 
CCAGGGATCA CAGGCAGCAT GGGACCGCAA GGAGCCCTGG GGCCACCTGG TGTCCCTGGA 
GCGAAGGGAG AGCGAGGTGA ACGGGGAGAC CTGCAGTCTC AAGCCATGGT GAGGGCGGTG 
GCACGTCAAG TATGCGAGCA GCTCATTCAG AGTCACATGG CCAGGTACAC AGCCATCCTC 
AACCAGATCC CCAGCCAGTC CTCATCTATC CGGACCATCC AGGGCCCTCC TGGGGAGCCC 
GGGAGACCAG GTTCACCTGG AACCCCTGGA GAACAAGGAC CCCCAGGTAC CCCAGGCTTC 
CCAGGAAATG CAGGAGTTCC GGGGACCCCA GGAGAACGAG GTCTAACTGG TGTCAAAGGA 
GAGAAAGGAA ATCCAGGCAT TGGAACTCAA GGCCCAAGAG GTCCCCCTGG GCCAGCAGGA 
CCTTCAGGGG AAAGTCGTCC TGGAAGTCCT GGGCCACCTG GCTCTCCTGG ACCAAGGGGT 
CCCCCGGGCC ACCTGGGAGT ACCTGGACCT CAGGGTCCTT CTGGCCAGCC TGGGTATTGT 
GACCCCTCCT CGTGCTCTGC TTATGGCGTT GGAGTGTCCC ATCCTGATCA GCCTGAGTTC 
ACACCGGTCC AGGACGAGCA GGAGGCCATG GATCTGTGGA GCGCAGGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col14a1 ( XM_006520385.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520448.1 Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant X2, mRNA.

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CloneID	OMu50907
Clone ID Related Accession (Same CDS sequence)	XM_006520385.3 , XM_006520385.4
Accession Version	XM_006520385.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XIV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTAGGAA CGGTTATGGT GCCTGGAGTG CAAAACAGTC TCCTTCTGAA GGCTCTACTT 
CCAGACACTG AGTATAAAGT CACAGTAACC CCTGTCTATA CTGTTGGAGA AGGCGTCAGC 
GTCTCCGCTC CAGGGAAGAC TTTGCCCTCC TCTGGTCCTC AAAACCTGAG GGTCTCAGAA 
GAATGGTACA ACCGGGTGCG CATTACATGG GACCCACCCT CTGGCCCCGT GAAGGGCTAT 
AGGATTGTCT ACAAACCTGT GAGTGTCCCT GGTCAAACTC TGGAGACCTT CGTGGGTGCT 
GACATCAACA CCATTGTCAT GACAAATCTC CTCAGTGGAA TGGACTACAA TGTGAAGATC 
TTTGCTTCCC AGGCGTCAGG CTTCAGTGAT GCTCTGACTG GCCTCGTGCA AACACTGTTC 
TTAGGGGTGA CAGATCTCCA AGCCAATCAG GTTGAAATGA CAAGCTTGTG TGCTCGGTGG 
CAAATACATC GCCATGCCAC AGCCTACAGG ATAGTTCTAG AATCCCTTCA GGACACACAA 
GCACAGGAAT CCACGGTGGG AGGAGGCGTA AACAGGCATT GCTTCTATGG ACTTCAGCCT 
GATTCAGAGT ATAAAATCAG TGTTTACACA AAGCTCCAGG AGCTAGAGGG ACCCAGTGTG 
AGCATCATGC AGAAAACACA GTCACTTCCC ACGGAACCAC CGACTTTTCC TCCAACCATC 
CCACCAGCAA AGGAAGTATG TAAAGCTGCC AAGGCAGACC TGGTATTCAT GGTGGATGGA 
TCTTGGAGCA TAGGAGATGA CAATTTCAAT AAAATCATCA ATTTTCTATA CAGCACTGTG 
GGAGCCCTGG ACAAGATTGG TGCAGATGGA ACTCAGGTGG CAATGGTTCA GTTCACTGAT 
GACCCCCGAA CAGAATTCAA ACTTGATTCT TACAAAACCA AAGAGACCCT TCTTGATGCA 
ATTAGACACA TTTCCTACAA AGGAGGAAAT ACTAAAACAG GAAAAGCTAT CAAGCATGTT 
CGGGATACCT TATTTACATC AGACTCAGGG ACAAGGAGAG GCATCCCAAA GGTCATCGTG 
GTGATAACTG ATGGACGGTC ACAAGATGAT GTCAACAAAA TCTCCAGGGA GATGCAGGCA 
GATGGTTTCA ACATCTTTGC CATCGGTGTG GCTGACGCTG ACTACTCCGA GTTGGTTCAG 
ATCGGCAGCA AGCCCAGCTC ACGGCATGTC TTCTTTGTGG ATGACTTTGA TGCCTTTAAG 
AAAATTGAAG ATGAATTGAT TACTTTTGTC TGTGAAACTG CATCAGCAAC CTGCCCAATG 
GTGCACAAGG ATGGAGTTGA TCTTGCAGGG TTTAAGATGA TGGAAATGTT TGGTTTGGTT 
GAAAAGGACT TTTCTGCAGT GGAAGGAGTA TCTATGGAAC CTGGTACCTT CAATTTGTTC 
CCATGCTATC AAATCCATAA GGATGCTCTG GTTTCTCAGC CAACAAAGTA TCTGCACCCA 
GAAGGATTGC CTTCTGACTA TACAATGAGT TTCCTGTTCC GGATTCTTCC TGACACACCA 
CAGGAGCCAT TTGCTCTTTG GGAGATTTTA AACAAAAATT CTGAGCCGCT AGTTGGGATC 
ATATTGGACA ATGGTGGGAA AACTCTAACT TATTTCAACT ATGACTATAC TGGAGATTTT 
CAAACTGTCA CTTTTGAAGG ACCCGACATC AGGAAAATGT TCTATGGAAG CTTTCACAAG 
CTCCATGTTG TTGTCAGCAA GACTCTGGCC AAAGTGGTTG TTGACTGCAA GGAAGTTGGT 
CAGAAGGCAA TAAATGCATC AGCGAATATC ACATCGGATG GTGTGGAAGT ACTAGGAAGA 
ATGGTCAGAT CAAGAGGACC AAATGGAAAC TCTGCACCAT TCCAGTTACA AATGTTTGAT 
ATCGTTTGCT CTACATCATG GGCCAGTAAA GACAGATGCT GCGAACTTCC TGGTCTGCGG 
GATGAAGAGT CCTGCCCAGA CCTTCCACGG TCCTGCTCTT GCTCGGAAAC CAATGAAGTG 
GCTCTAGGAC CAGCTGGCCC CCCAGGTGGT CCAGGACTCC GAGGACCAAA AGGCCAACAA 
GGTGAACAGG GTCCGAAGGG ACCAGAGGGA CCTCGGGGAG AAACAGGTCC TGCAGGACCC 
CAGGGCCCCC CTGGACCCCA GGGACCTAGT GGTCTGTCCA TTCAAGGCAT GCCTGGAATG 
CCGGGTGATA AAGGAGATAA AGGAGATGCT GGTCTTCCTG GTCCACAGGG TGTCCCTGGA 
GGTGTTGGTT CACCAGGCCG TGATGGCTCA CCAGGCCAGA GGGGATTTCC CGGGAAGGAC 
GGTTCCTCTG GGCCTCCAGG ACCACCTGGG CCAATTGGCA TTCCTGGAGC CCCTGGTGTC 
CCAGGGATCA CAGGCAGCAT GGGACCGCAA GGAGCCCTGG GGCCACCTGG TGTCCCTGGA 
GCGAAGGGAG AGCGAGGTGA ACGGGGAGAC CTGCAGTCTC AAGCCATGGT GAGGGCGGTG 
GCACGTCAAG TATGCGAGCA GCTCATTCAG AGTCACATGG CCAGGTACAC AGCCATCCTC 
AACCAGATCC CCAGCCAGTC CTCATCTATC CGGACCATCC AGGGCCCTCC TGGGGAGCCC 
GGGAGACCAG GTTCACCTGG AACCCCTGGA GAACAAGGAC CCCCAGGTAC CCCAGGCTTC 
CCAGGAAATG CAGGAGTTCC GGGGACCCCA GGAGAACGAG GTCTAACTGG TGTCAAAGGA 
GAGAAAGGAA ATCCAGGCAT TGGAACTCAA GGCCCAAGAG GTCCCCCTGG GCCAGCAGGA 
CCTTCAGGGG AAAGTCGTCC TGGAAGTCCT GGGCCACCTG GCTCTCCTGG ACCAAGGGGT 
CCCCCGGGCC ACCTGGGAGT ACCTGGACCT CAGGGTCCTT CTGGCCAGCC TGGGTATTGT 
GACCCCTCCT CGTGCTCTGC TTATGGCGTT GGAGATCTCA TCCCGTACAA TGATTACCAG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520448.1
CDS	328..5673
Translation	MMIWQCKMRDWLILAFLAAACFCTIVRGQVAPPTRLRYNVISHDSIQISWKAPRGKFGGYKLLVAPASGGKTNQMNLQNTATKAIIQGLLPEQNYTVQLIAYYKDKESKPAQGQFRIKDLEKRKDPTKPKVKVVDKGNGSKPTSPEEVKFFCETPAIADIVILVDGSWSIGRFNFRLVRNFLENLVTAFNVGSEKTRIGLAQYSGDPRIEWHLNAFNTKDEVIDAVRSLPYKGGNTLTGLALNFIFENSFKPEAGSRSGVSKIGILITDGKSQDDIIPPSRNLRESGVELFAIGVKNADLSELQEIASEPDSTHVYNVAEFDLMHTVVESLTRTVCSRVEEQDKEIKASALATIGPPTELITSEVTARSFMVNWTQSPGKVEKYRVVYYPTRGGKPEEVVVDGSVSSTVLKNLMSSTEYQIAVFAVSAHTASEGLRGAETTLALPMASDLELYDVTENSMRVRWDAVPGATGYLILYAPLTEGLAGDEKEMKIGETHTDIELSGLFPNTEYTVTVYAMFGEEASDPATGQETTLPLTPPRNLRISNVGSNSARLTWDPASGKISGYRIVYTSADGTEINEVEVDPITTFPLKGLTPLTEYSIAIFSIYEEGQSLPLVGEFTTEEVPAQQYLEIDEVKTDSFRVTWHPLSAEEGQHKLMWIPVYGGKTQEVDLKEEQDSYVIEGLDPGTEYEVSLLAVLDDGSESEVVTAVGTTLDDFWTEAPTAIEPTSPVTSVLQTGIRNLVVDDETATSLRVSWDISDSNVEQFRVTYLKAQGDPMEEVVGTVMVPGVQNSLLLKALLPDTEYKVTVTPVYTVGEGVSVSAPGKTLPSSGPQNLRVSEEWYNRVRITWDPPSGPVKGYRIVYKPVSVPGQTLETFVGADINTIVMTNLLSGMDYNVKIFASQASGFSDALTGLVQTLFLGVTDLQANQVEMTSLCARWQIHRHATAYRIVLESLQDTQAQESTVGGGVNRHCFYGLQPDSEYKISVYTKLQELEGPSVSIMQKTQSLPTEPPTFPPTIPPAKEVCKAAKADLVFMVDGSWSIGDDNFNKIINFLYSTVGALDKIGADGTQVAMVQFTDDPRTEFKLDSYKTKETLLDAIRHISYKGGNTKTGKAIKHVRDTLFTSDSGTRRGIPKVIVVITDGRSQDDVNKISREMQADGFNIFAIGVADADYSELVQIGSKPSSRHVFFVDDFDAFKKIEDELITFVCETASATCPMVHKDGVDLAGFKMMEMFGLVEKDFSAVEGVSMEPGTFNLFPCYQIHKDALVSQPTKYLHPEGLPSDYTMSFLFRILPDTPQEPFALWEILNKNSEPLVGIILDNGGKTLTYFNYDYTGDFQTVTFEGPDIRKMFYGSFHKLHVVVSKTLAKVVVDCKEVGQKAINASANITSDGVEVLGRMVRSRGPNGNSAPFQLQMFDIVCSTSWASKDRCCELPGLRDEESCPDLPRSCSCSETNEVALGPAGPPGGPGLRGPKGQQGEQGPKGPEGPRGETGPAGPQGPPGPQGPSGLSIQGMPGMPGDKGDKGDAGLPGPQGVPGGVGSPGRDGSPGQRGFPGKDGSSGPPGPPGPIGIPGAPGVPGITGSMGPQGALGPPGVPGAKGERGERGDLQSQAMVRAVARQVCEQLIQSHMARYTAILNQIPSQSSSIRTIQGPPGEPGRPGSPGTPGEQGPPGTPGFPGNAGVPGTPGERGLTGVKGEKGNPGIGTQGPRGPPGPAGPSGESRPGSPGPPGSPGPRGPPGHLGVPGPQGPSGQPGYCDPSSCSAYGVGDLIPYNDYQH

Target ORF information:

RefSeq Version	XM_006520385.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XIV, alpha 1 (Col14a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520385.3

ORF Insert Sequence:

ATGATGATTT GGCAGTGCAA GATGCGGGAC TGGCTGATTC TAGCTTTTTT GGCGGCTGCT 
TGTTTCTGCA CCATTGTCAG GGGACAAGTG GCTCCACCCA CAAGGTTAAG ATATAATGTG 
ATTTCCCATG ATAGTATACA GATTTCATGG AAAGCACCAA GAGGGAAATT TGGTGGCTAC 
AAACTGCTCG TGGCTCCAGC TTCAGGAGGA AAGACCAACC AGATGAATCT CCAGAACACT 
GCAACAAAAG CGATTATTCA AGGCCTTCTG CCAGAGCAGA ACTACACAGT TCAACTCATT 
GCCTACTATA AAGACAAGGA AAGCAAGCCC GCTCAAGGCC AGTTCAGAAT TAAAGATTTA 
GAGAAAAGAA AGGATCCAAC AAAGCCCAAA GTCAAGGTTG TGGACAAAGG AAATGGAAGT 
AAACCAACTT CACCAGAAGA GGTAAAATTC TTCTGTGAGA CTCCAGCCAT CGCTGACATC 
GTCATCCTGG TGGATGGGTC GTGGAGTATT GGGAGGTTCA ACTTCAGGCT TGTGCGCAAT 
TTCTTGGAAA ACCTGGTCAC AGCGTTCAAC GTGGGCTCAG AAAAGACAAG AATCGGCTTG 
GCACAGTACA GTGGGGACCC CAGAATAGAG TGGCACTTGA ATGCCTTTAA CACAAAAGAT 
GAGGTGATTG ATGCTGTCCG AAGCCTTCCA TACAAAGGAG GAAATACCCT AACAGGTCTT 
GCTTTGAACT TTATTTTTGA GAATAGCTTT AAACCAGAAG CAGGATCGAG GAGTGGAGTT 
TCCAAGATTG GCATTTTGAT CACAGATGGA AAATCCCAGG ATGATATTAT CCCACCATCT 
AGAAACCTCC GTGAGTCTGG TGTAGAGCTG TTTGCCATAG GGGTTAAAAA TGCCGACCTG 
AGTGAGCTGC AGGAGATCGC CTCTGAGCCA GACAGCACCC ATGTGTACAA TGTTGCTGAG 
TTCGACCTGA TGCACACAGT GGTGGAGAGC CTGACCCGGA CTGTATGCTC TAGAGTGGAA 
GAGCAGGACA AGGAGATTAA AGCCTCAGCC CTTGCCACCA TCGGGCCACC TACAGAGCTG 
ATCACTTCTG AAGTCACTGC CAGGAGCTTT ATGGTTAACT GGACTCAGTC CCCAGGAAAA 
GTGGAAAAAT ACAGAGTTGT GTATTATCCC ACCAGAGGCG GAAAGCCGGA GGAGGTGGTG 
GTGGATGGGA GTGTATCTTC CACAGTGTTG AAAAACTTGA TGTCTTCAAC TGAGTACCAG 
ATAGCAGTGT TTGCAGTATC TGCCCACACA GCTAGTGAAG GCCTGCGGGG AGCAGAGACC 
ACATTGGCCT TGCCCATGGC TTCCGACCTC GAGCTGTACG ACGTGACTGA GAACAGCATG 
CGCGTCAGGT GGGATGCAGT ACCTGGAGCC ACAGGCTATC TGATCCTCTA TGCTCCTCTC 
ACAGAAGGTC TGGCTGGAGA TGAGAAAGAG ATGAAAATTG GAGAGACACA CACAGACATT 
GAACTGAGTG GGCTATTCCC CAATACAGAA TATACAGTCA CTGTGTATGC CATGTTTGGA 
GAAGAGGCCA GCGATCCTGC TACAGGACAA GAGACAACCC TACCCTTAAC CCCACCAAGA 
AATTTGCGGA TCTCCAATGT TGGCTCTAAC AGCGCTCGGT TAACCTGGGA CCCCGCATCG 
GGAAAGATCA GTGGTTACAG AATTGTGTAT ACCAGTGCAG ACGGGACAGA AATCAATGAG 
GTTGAAGTCG ATCCTATCAC TACATTCCCT CTGAAAGGCT TGACCCCCCT CACGGAGTAC 
TCCATTGCAA TCTTCTCCAT CTACGAGGAG GGGCAGTCCT TGCCCCTGGT TGGAGAGTTT 
ACCACAGAGG AAGTTCCCGC CCAGCAATAC TTGGAAATTG ATGAGGTGAA GACAGACAGT 
TTTCGAGTGA CTTGGCATCC GCTCTCCGCT GAGGAGGGGC AGCACAAGCT GATGTGGATT 
CCGGTCTATG GCGGGAAGAC CCAGGAGGTT GACCTCAAAG AAGAACAGGA CTCTTATGTT 
ATTGAAGGCC TGGATCCTGG AACCGAGTAC GAAGTCTCCC TGTTAGCTGT GCTTGATGAC 
GGAAGCGAGA GTGAGGTGGT GACCGCAGTG GGAACCACAC TTGATGATTT TTGGACTGAA 
GCACCCACAG CCATAGAACC TACCAGCCCT GTGACTTCAG TTCTCCAGAC AGGAATCAGA 
AACCTGGTTG TAGATGACGA AACTGCTACT AGCCTGCGGG TATCGTGGGA CATTTCTGAC 
AGCAATGTGG AGCAGTTTAG GGTGACCTAC CTGAAAGCCC AAGGGGACCC TATGGAAGAA 
GTGGTAGGAA CGGTTATGGT GCCTGGAGTG CAAAACAGTC TCCTTCTGAA GGCTCTACTT 
CCAGACACTG AGTATAAAGT CACAGTAACC CCTGTCTATA CTGTTGGAGA AGGCGTCAGC 
GTCTCCGCTC CAGGGAAGAC TTTGCCCTCC TCTGGTCCTC AAAACCTGAG GGTCTCAGAA 
GAATGGTACA ACCGGGTGCG CATTACATGG GACCCACCCT CTGGCCCCGT GAAGGGCTAT 
AGGATTGTCT ACAAACCTGT GAGTGTCCCT GGTCAAACTC TGGAGACCTT CGTGGGTGCT 
GACATCAACA CCATTGTCAT GACAAATCTC CTCAGTGGAA TGGACTACAA TGTGAAGATC 
TTTGCTTCCC AGGCGTCAGG CTTCAGTGAT GCTCTGACTG GCCTCGTGCA AACACTGTTC 
TTAGGGGTGA CAGATCTCCA AGCCAATCAG GTTGAAATGA CAAGCTTGTG TGCTCGGTGG 
CAAATACATC GCCATGCCAC AGCCTACAGG ATAGTTCTAG AATCCCTTCA GGACACACAA 
GCACAGGAAT CCACGGTGGG AGGAGGCGTA AACAGGCATT GCTTCTATGG ACTTCAGCCT 
GATTCAGAGT ATAAAATCAG TGTTTACACA AAGCTCCAGG AGCTAGAGGG ACCCAGTGTG 
AGCATCATGC AGAAAACACA GTCACTTCCC ACGGAACCAC CGACTTTTCC TCCAACCATC 
CCACCAGCAA AGGAAGTATG TAAAGCTGCC AAGGCAGACC TGGTATTCAT GGTGGATGGA 
TCTTGGAGCA TAGGAGATGA CAATTTCAAT AAAATCATCA ATTTTCTATA CAGCACTGTG 
GGAGCCCTGG ACAAGATTGG TGCAGATGGA ACTCAGGTGG CAATGGTTCA GTTCACTGAT 
GACCCCCGAA CAGAATTCAA ACTTGATTCT TACAAAACCA AAGAGACCCT TCTTGATGCA 
ATTAGACACA TTTCCTACAA AGGAGGAAAT ACTAAAACAG GAAAAGCTAT CAAGCATGTT 
CGGGATACCT TATTTACATC AGACTCAGGG ACAAGGAGAG GCATCCCAAA GGTCATCGTG 
GTGATAACTG ATGGACGGTC ACAAGATGAT GTCAACAAAA TCTCCAGGGA GATGCAGGCA 
GATGGTTTCA ACATCTTTGC CATCGGTGTG GCTGACGCTG ACTACTCCGA GTTGGTTCAG 
ATCGGCAGCA AGCCCAGCTC ACGGCATGTC TTCTTTGTGG ATGACTTTGA TGCCTTTAAG 
AAAATTGAAG ATGAATTGAT TACTTTTGTC TGTGAAACTG CATCAGCAAC CTGCCCAATG 
GTGCACAAGG ATGGAGTTGA TCTTGCAGGG TTTAAGATGA TGGAAATGTT TGGTTTGGTT 
GAAAAGGACT TTTCTGCAGT GGAAGGAGTA TCTATGGAAC CTGGTACCTT CAATTTGTTC 
CCATGCTATC AAATCCATAA GGATGCTCTG GTTTCTCAGC CAACAAAGTA TCTGCACCCA 
GAAGGATTGC CTTCTGACTA TACAATGAGT TTCCTGTTCC GGATTCTTCC TGACACACCA 
CAGGAGCCAT TTGCTCTTTG GGAGATTTTA AACAAAAATT CTGAGCCGCT AGTTGGGATC 
ATATTGGACA ATGGTGGGAA AACTCTAACT TATTTCAACT ATGACTATAC TGGAGATTTT 
CAAACTGTCA CTTTTGAAGG ACCCGACATC AGGAAAATGT TCTATGGAAG CTTTCACAAG 
CTCCATGTTG TTGTCAGCAA GACTCTGGCC AAAGTGGTTG TTGACTGCAA GGAAGTTGGT 
CAGAAGGCAA TAAATGCATC AGCGAATATC ACATCGGATG GTGTGGAAGT ACTAGGAAGA 
ATGGTCAGAT CAAGAGGACC AAATGGAAAC TCTGCACCAT TCCAGTTACA AATGTTTGAT 
ATCGTTTGCT CTACATCATG GGCCAGTAAA GACAGATGCT GCGAACTTCC TGGTCTGCGG 
GATGAAGAGT CCTGCCCAGA CCTTCCACGG TCCTGCTCTT GCTCGGAAAC CAATGAAGTG 
GCTCTAGGAC CAGCTGGCCC CCCAGGTGGT CCAGGACTCC GAGGACCAAA AGGCCAACAA 
GGTGAACAGG GTCCGAAGGG ACCAGAGGGA CCTCGGGGAG AAACAGGTCC TGCAGGACCC 
CAGGGCCCCC CTGGACCCCA GGGACCTAGT GGTCTGTCCA TTCAAGGCAT GCCTGGAATG 
CCGGGTGATA AAGGAGATAA AGGAGATGCT GGTCTTCCTG GTCCACAGGG TGTCCCTGGA 
GGTGTTGGTT CACCAGGCCG TGATGGCTCA CCAGGCCAGA GGGGATTTCC CGGGAAGGAC 
GGTTCCTCTG GGCCTCCAGG ACCACCTGGG CCAATTGGCA TTCCTGGAGC CCCTGGTGTC 
CCAGGGATCA CAGGCAGCAT GGGACCGCAA GGAGCCCTGG GGCCACCTGG TGTCCCTGGA 
GCGAAGGGAG AGCGAGGTGA ACGGGGAGAC CTGCAGTCTC AAGCCATGGT GAGGGCGGTG 
GCACGTCAAG TATGCGAGCA GCTCATTCAG AGTCACATGG CCAGGTACAC AGCCATCCTC 
AACCAGATCC CCAGCCAGTC CTCATCTATC CGGACCATCC AGGGCCCTCC TGGGGAGCCC 
GGGAGACCAG GTTCACCTGG AACCCCTGGA GAACAAGGAC CCCCAGGTAC CCCAGGCTTC 
CCAGGAAATG CAGGAGTTCC GGGGACCCCA GGAGAACGAG GTCTAACTGG TGTCAAAGGA 
GAGAAAGGAA ATCCAGGCAT TGGAACTCAA GGCCCAAGAG GTCCCCCTGG GCCAGCAGGA 
CCTTCAGGGG AAAGTCGTCC TGGAAGTCCT GGGCCACCTG GCTCTCCTGG ACCAAGGGGT 
CCCCCGGGCC ACCTGGGAGT ACCTGGACCT CAGGGTCCTT CTGGCCAGCC TGGGTATTGT 
GACCCCTCCT CGTGCTCTGC TTATGGCGTT GGAGATCTCA TCCCGTACAA TGATTACCAG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Odd skipped-related 1 identifies a population of embryonic fibro-adipogenic progenitors regulating myogenesis during limb development.
Nature communications8(1)1218(2017 Oct)
Vallecillo-Garc?a P,Orgeur M,Vom Hofe-Schneider S,Stumm J,Kappert V,Ibrahim DM,B?rno ST,Hayashi S,Relaix F,Hildebrandt K,Sengle G,Koch M,Timmermann B,Marazzi G,Sassoon DA,Duprez D,Stricker S

Scleraxis is required for maturation of tissue domains for proper integration of the musculoskeletal system.
Scientific reports745010(2017 Mar)
Yoshimoto Y,Takimoto A,Watanabe H,Hiraki Y,Kondoh G,Shukunami C

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

Regulators of Collagen Fibrillogenesis during Molar Development in the Mouse.
Frontiers in physiology8554(2017)
Zvackova I,Matalova E,Lesot H

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Col14a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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