Clcn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clcn1
Entrez Gene ID	12723
Full Name	chloride channel, voltage-sensitive 1
Synonyms	Clc-1,Clc1,SMCC1,adr,mto,myotonia,nmf355
General protein information	Preferred Names chloride channel, voltage-sensitive 1 Names chloride channel protein 1 arrested development of righting response chloride channel 1 chloride channel protein, skeletal muscle
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 B2.1\|6 20.57 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013491.2	NP_038519.1	chloride channel protein 1
XM_011241199.1	XP_011239501.1	chloride channel protein 1 isoform X1
XM_006505476.3	XP_006505539.1	chloride channel protein 1 isoform X1
NM_013491.3	NP_038519.1	chloride channel protein 1 isoform 1
XM_006505477.3	XP_006505540.1	chloride channel protein 1 isoform X3
NM_001363712.1	NP_001350641.1	chloride channel protein 1 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Clcn1	NP_038519.1
Gallus gallus (chicken)	CLCN1	XP_004938180.1
Danio rerio (zebrafish)	LOC567478	XP_695866.4
	CLCN1	XP_001092282.1
Danio rerio (zebrafish)	clcn1a	NP_001076379.1
Homo sapiens (human)	CLCN1	NP_000074.2
Pan troglodytes (chimpanzee)	CLCN1	XP_527935.2
Canis lupus familiaris (dog)	CLCN1	NP_001003124.1
Bos taurus (cattle)	CLCN1	NP_001137343.1
Rattus norvegicus (Norway rat)	Clcn1	NP_037279.1

Gene Ontology
Bibliography

Related articles in PubMed

Dmpk gene deletion or antisense knockdown does not compromise cardiac or skeletal muscle function in mice.
Carrell ST, Carrell EM, Auerbach D, Pandey SK, Bennett CF, Dirksen RT, Thornton CA
Human molecular genetics25(19)4328-4338(2016 10)

Variant mapping and mutation discovery in inbred mice using next-generation sequencing.
Gallego-Llamas J, Timms AE, Geister KA, Lindsay A, Beier DR
BMC genomics16913(2015 Nov)

Cooperation meets competition in microRNA-mediated DMPK transcript regulation.
Koscianska E, Witkos TM, Kozlowska E, Wojciechowska M, Krzyzosiak WJ
Nucleic acids research43(19)9500-18(2015 Oct)

Muscleblind-Like 1 and Muscleblind-Like 3 Depletion Synergistically Enhances Myotonia by Altering Clc-1 RNA Translation.
Choi J, Personius KE, DiFranco M, Dansithong W, Yu C, Srivastava S, Dixon DM, Bhatt DB, Comai L, Vergara JL, Reddy S
EBioMedicine2(9)1034-47(2015 Sep)

NKX2-5, a modifier of skeletal muscle pathology due to RNA toxicity.
Gladman JT, Yadava RS, Mandal M, Yu Q, Kim YK, Mahadevan MS
Human molecular genetics24(1)251-64(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Muscleblind-like 1 (Mbnl1) and Muscleblind-Like 3 (Mbnl3) bind skeletal muscle chloride channel CIC-1 (Clc-1) mRNA.
Title: Muscleblind-Like 1 and Muscleblind-Like 3 Depletion Synergistically Enhances Myotonia by Altering Clc-1 RNA Translation.

Sex hormones at high concentration can rapidly modulate ClC-1 in mouse skeletal muscle fibers in vitro.
Title: Nongenomic actions of progesterone and 17?-estradiol on the chloride conductance of skeletal muscle.

The expression of the muscle chloride channel, ClC-1, in Huntington disease muscle was compromised by improper splicing and a corresponding reduction in total Clcn1 (gene for ClC-1) mRNA.
Title: Huntington disease skeletal muscle is hyperexcitable owing to chloride and potassium channel dysfunction.

Myotonia in adult human skeletal actin transgenic mice may be explained on the basis of a mosaic expression of ClC-1 channels in different fibres and/or on alterations of other conductances.
Title: Age-dependent chloride channel expression in skeletal muscle fibres of normal and HSA(LR) myotonic mice.

Myotonia (delayed muscle relaxation) is the most commonly observed symptom in DM1 patients and is caused by aberrant splicing of the skeletal muscle chloride channel (CLCN1) gene
Title: Manumycin A corrects aberrant splicing of Clcn1 in myotonic dystrophy type 1 (DM1) mice.

The following Clcn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clcn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu36846	XM_006505476.3 Latest version!	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu36847	XM_006505477.3 Latest version!	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu15086	NM_013491.3 Latest version!	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$580.30 $829.00
OMu67375	NM_001363712.1 Latest version!	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu67375	XM_011241199.1 Latest version!	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OMu15086	NM_013491.2	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$580.30 $829.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clcn1 ( XM_006505476.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505539.1 Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu36846
Clone ID Related Accession (Same CDS sequence)	XM_006505476.3
Accession Version	XM_006505476.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chloride channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006505476.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTCC CCTTTGACCT GAAGGAACTC CCAGCTTTTG CTGTCATTGG GATTTGCTGC 
GGGTTCTTGG GAGCTGTTTT CGTTTATCTG CATCGCCAAG TCATGCTCGG TGTCCGAAAG 
CACAAGTGTC TCAGCCAATT TCTTGCTAAG CACCGCCTGC TATATCCTGG AATTGTTACC 
TTTGTCATCG CCTCGCTCAC ATTTCCACCA GGAATGGGTC AATTCATGGC TGGAGAGCTG 
ATGCCCCGTG AAGCTATCAG TACCCTCTTT GACAACAACA CATGGGTAAA GCACATAGGT 
GACCCCCAAA GCTTGGGCCA GTCAGCTGTG TGGCTTCACC CCCAGGTCAA CGTGATCATC 
ATCATCCTTC TCTTCTTCGT TATGAAGTTT TGGATGTCCA TTGTAGCCAC CACTATGCCC 
ATACCCTGTG GAGGCTTCAT GCCTGTTTTT GTGCTAGGAG CTGCATTTGG AAGGCTGGTA 
GGAGAGATCA TGGCCATGCT ATTCCCTGAG GGTATCTTAT TTGATGATAT CATCTATAAG 
ATCTTACCTG GGGGCTATGC AGTAATTGGA GCAGCAGCTT TGACAGGGGC TGTCTCCCAC 
ACAGTCTCCA CAGCCGTCAT TTGCTTCGAA TTAACCGGTC AGATTGCTCA CATCCTGCCC 
ATGATGGTGG CTGTTATCTT GGCCAACATG GTGGCTCAGA GTCTGCAGCC CTCCCTCTAT 
GACAGCATCA TCCAGGTCAA GAAGCTTCCC TATCTGCCAG ACCTTGGTTG GAACCAGCTC 
AGCAAATTTA CAATTTTTGT TGAGGACATC ATGGTACGTG ATGTGAAGTT CGTTTCAGCT 
TCTTGTACAT ATGGGGAACT GAGAAACCTA CTCCAGGCCA CCACAGTCAA GACTTTACCA 
TTGGTTGACT CCAAAGATTC AATGATCCTG CTGGGCTCTG TGGAACGCTC AGAACTGCAG 
TCCCTCCTGC AGCGCCACCT GTGTGCAGAG CGAAGGTTGA AGGCTGCCCA GGACATGGCT 
CGAAAGTTAT CAGAGCTGCC TTATAATGGC AAGGCTCAGC TGGCTGGGGA CTGGCATCCT 
GGTGGTCGAC CTGAGTCCTT TGCCTTCGTA GATGAAGATG AAGATGAGGA CCTCTCCAGG 
AAGATGGAGC TTCCACTGAC TCCAGCTCCT CCCCCTCCTT CCCCTCCTCC TCCACCTTCC 
CAGTTTCCTA TTGCTCCATC AAACCCTGAA GAACCTAATG GGCCACTGCC CAGCCATAAA 
CAGCCACCTG AAGCCTCAGA CTCTGCAGAT CAAAGATCAT CCACCTTCCA ACGCCTGCTG 
CACTGCTTGC TAGGAAAAGC TCACTCCAAA AAGAAGAAAA TAACACAGGA TTCAACAGAT 
TTAGTGGATA ACATGTCACC TGAAGAGATT GAGGCCTGGG AGCGGGAGCA GCTGAGCCAG 
CCTGTGTGCT TTGATTGCTG CTGCATCGAC CAGTCTCCCT TCCAGCTGGT GGAGCAGACA 
ACCCTGCACA AGACTCATAC ACTCTTCTCA CTTCTTGGCC TCCATCTTGC CTATGTGACC 
AGCATGGGGA AGCTCAGAGG TGTCTTGGCA CTAGAAGAGC TACAGAAAGC TATTGAGGGC 
CACACCAAAT CTGGGGTGCA GCTTCGCCCT CCACTTGCCA GCTTCCGGAA TACAACTTCA 
ATCCGGAAGA CTCCTGGGGG GCCACCCCCT CCTGCAGAGG GCTGGAATGT ACCTGAGGAT 
GGAGATGGGG CTCCTGGAAG AGAAGTCATG GTTCCTACCA TGCCAGAGAC TCCTGTCCCA 
CCACCATCTC CAGAGGCCCC TTCCTGCCTG GCCCCAGCCA GAGCGGAGGG TGAGCTGGAG 
GAACTGGAGA TGGTGGGGAG CCTAGAGCCT GAGGAGGAGC TGGCTGACAT CTTGCATGGC 
CCCAGTCTGC GGTCCACTGA TGAGGAAGAT GAGGACGAGC TGATCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505539.1
CDS	256..2226
Translation	MDFPFDLKELPAFAVIGICCGFLGAVFVYLHRQVMLGVRKHKCLSQFLAKHRLLYPGIVTFVIASLTFPPGMGQFMAGELMPREAISTLFDNNTWVKHIGDPQSLGQSAVWLHPQVNVIIIILLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPEGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKFTIFVEDIMVRDVKFVSASCTYGELRNLLQATTVKTLPLVDSKDSMILLGSVERSELQSLLQRHLCAERRLKAAQDMARKLSELPYNGKAQLAGDWHPGGRPESFAFVDEDEDEDLSRKMELPLTPAPPPPSPPPPPSQFPIAPSNPEEPNGPLPSHKQPPEASDSADQRSSTFQRLLHCLLGKAHSKKKKITQDSTDLVDNMSPEEIEAWEREQLSQPVCFDCCCIDQSPFQLVEQTTLHKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSIRKTPGGPPPPAEGWNVPEDGDGAPGREVMVPTMPETPVPPPSPEAPSCLAPARAEGELEELEMVGSLEPEEELADILHGPSLRSTDEEDEDELIL

Target ORF information:

RefSeq Version	XM_006505476.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505476.3

ORF Insert Sequence:

ATGGATTTCC CCTTTGACCT GAAGGAACTC CCAGCTTTTG CTGTCATTGG GATTTGCTGC 
GGGTTCTTGG GAGCTGTTTT CGTTTATCTG CATCGCCAAG TCATGCTCGG TGTCCGAAAG 
CACAAGTGTC TCAGCCAATT TCTTGCTAAG CACCGCCTGC TATATCCTGG AATTGTTACC 
TTTGTCATCG CCTCGCTCAC ATTTCCACCA GGAATGGGTC AATTCATGGC TGGAGAGCTG 
ATGCCCCGTG AAGCTATCAG TACCCTCTTT GACAACAACA CATGGGTAAA GCACATAGGT 
GACCCCCAAA GCTTGGGCCA GTCAGCTGTG TGGCTTCACC CCCAGGTCAA CGTGATCATC 
ATCATCCTTC TCTTCTTCGT TATGAAGTTT TGGATGTCCA TTGTAGCCAC CACTATGCCC 
ATACCCTGTG GAGGCTTCAT GCCTGTTTTT GTGCTAGGAG CTGCATTTGG AAGGCTGGTA 
GGAGAGATCA TGGCCATGCT ATTCCCTGAG GGTATCTTAT TTGATGATAT CATCTATAAG 
ATCTTACCTG GGGGCTATGC AGTAATTGGA GCAGCAGCTT TGACAGGGGC TGTCTCCCAC 
ACAGTCTCCA CAGCCGTCAT TTGCTTCGAA TTAACCGGTC AGATTGCTCA CATCCTGCCC 
ATGATGGTGG CTGTTATCTT GGCCAACATG GTGGCTCAGA GTCTGCAGCC CTCCCTCTAT 
GACAGCATCA TCCAGGTCAA GAAGCTTCCC TATCTGCCAG ACCTTGGTTG GAACCAGCTC 
AGCAAATTTA CAATTTTTGT TGAGGACATC ATGGTACGTG ATGTGAAGTT CGTTTCAGCT 
TCTTGTACAT ATGGGGAACT GAGAAACCTA CTCCAGGCCA CCACAGTCAA GACTTTACCA 
TTGGTTGACT CCAAAGATTC AATGATCCTG CTGGGCTCTG TGGAACGCTC AGAACTGCAG 
TCCCTCCTGC AGCGCCACCT GTGTGCAGAG CGAAGGTTGA AGGCTGCCCA GGACATGGCT 
CGAAAGTTAT CAGAGCTGCC TTATAATGGC AAGGCTCAGC TGGCTGGGGA CTGGCATCCT 
GGTGGTCGAC CTGAGTCCTT TGCCTTCGTA GATGAAGATG AAGATGAGGA CCTCTCCAGG 
AAGATGGAGC TTCCACTGAC TCCAGCTCCT CCCCCTCCTT CCCCTCCTCC TCCACCTTCC 
CAGTTTCCTA TTGCTCCATC AAACCCTGAA GAACCTAATG GGCCACTGCC CAGCCATAAA 
CAGCCACCTG AAGCCTCAGA CTCTGCAGAT CAAAGATCAT CCACCTTCCA ACGCCTGCTG 
CACTGCTTGC TAGGAAAAGC TCACTCCAAA AAGAAGAAAA TAACACAGGA TTCAACAGAT 
TTAGTGGATA ACATGTCACC TGAAGAGATT GAGGCCTGGG AGCGGGAGCA GCTGAGCCAG 
CCTGTGTGCT TTGATTGCTG CTGCATCGAC CAGTCTCCCT TCCAGCTGGT GGAGCAGACA 
ACCCTGCACA AGACTCATAC ACTCTTCTCA CTTCTTGGCC TCCATCTTGC CTATGTGACC 
AGCATGGGGA AGCTCAGAGG TGTCTTGGCA CTAGAAGAGC TACAGAAAGC TATTGAGGGC 
CACACCAAAT CTGGGGTGCA GCTTCGCCCT CCACTTGCCA GCTTCCGGAA TACAACTTCA 
ATCCGGAAGA CTCCTGGGGG GCCACCCCCT CCTGCAGAGG GCTGGAATGT ACCTGAGGAT 
GGAGATGGGG CTCCTGGAAG AGAAGTCATG GTTCCTACCA TGCCAGAGAC TCCTGTCCCA 
CCACCATCTC CAGAGGCCCC TTCCTGCCTG GCCCCAGCCA GAGCGGAGGG TGAGCTGGAG 
GAACTGGAGA TGGTGGGGAG CCTAGAGCCT GAGGAGGAGC TGGCTGACAT CTTGCATGGC 
CCCAGTCTGC GGTCCACTGA TGAGGAAGAT GAGGACGAGC TGATCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn1 ( XM_006505477.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505540.1 Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu36847
Clone ID Related Accession (Same CDS sequence)	XM_006505477.3
Accession Version	XM_006505477.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chloride channel protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006505477.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGT CCCAGTCCCA GCGGCATGGA GGGGAACAGA GCTGGTGGGG CAGTGCCCCC 
CAGTACCAGT ACATGCCCTT TGAACATTGT ACCAGCTACG GACTGCCCTC AGAGAATGGG 
GGCCTTCAGC ACCGGCCCCG AAAGGACATG GGTCCCAGGC ACAATGCCCA CCCAACACAG 
ATATATGGCC ATCAAAAAGA ACAATATTCA TATAAGGCAC AGGACGGGGG AATGCCCAAG 
AAGATGGGCT CCAGTTCTAC CATGGACAGC TTGGATGAGG ACCACTATTC TAAATGTCAA 
GACTGTGTCC ATCGCCTGGG ACGTGTGCTG AGAAGGAAGC TGGGGGAAGA TTGGATCTTT 
CTTGTGCTCC TGGGCCTCCT GATGGCTCTA GTCAGCTGGT GCATGGACTA TGTTAGTGCC 
AAGAGCCTTC AGGCCTACAA GTGGACCTAT GCCCAGATGA AGCCTAGCCT TCCTCTGCAG 
TACCTGGCCT GGGTCACCTT CCCACTTATC CTGATCCTCT TCAGCGCCCT CTTTTGCCAA 
CTCATCTCTC CCCAGGCTGT GGGCTCTGGA ATCCCTGAGA TGAAAACAAT TCTTCGTGGT 
GTTGTCTTGA AGGAATACCT CACACTCAAG GCCTTTGTAG CCAAGGTGGT AGCTTTGACA 
GCTGGACTGG GCAGTGGCAT CCCTGTGGGG AAAGAGGGTC CCTTTGTTCA CATCGCCAGC 
ATCTGTGCTG CTGTCCTCAG CAAGTTTATG TCCATGTTCT CTGGTGTCTA TGAGCAGCCA 
TACTATTACA CTGACATCCT GACAGTGGGC TGTGCCGTGG GGGTCGGTTG CTGTTTTGGA 
ACACCACTTG GAGGAGTGCT ATTTAGCATC GAGGTCACCT CTACCTACTT TGCTGTTCGG 
AATTACTGGC GAGGATTCTT TGCAGCCACA TTCAGTGCCT TTGTGTTCCG TGTCCTGGCC 
GTTTGGAACA AGGATGCTGT CACCATCACT GCTCTGTTCA GAACGAATTT CCGAATGGAT 
TTCCCCTTTG ACCTGAAGGA ACTCCCAGCT TTTGCTGTCA TTGGGATTTG CTGCGGGTTC 
TTGGGAGCTG TTTTCGTTTA TCTGCATCGC CAAGTCATGC TCGGTGTCCG AAAGCACAAG 
TGTCTCAGCC AATTTCTTGC TAAGCACCGC CTGCTATATC CTGGAATTGT TACCTTTGTC 
ATCGCCTCGC TCACATTTCC ACCAGGAATG GGTCAATTCA TGGCTGGAGA GCTGATGCCC 
CGTGAAGCTA TCAGTACCCT CTTTGACAAC AACACATGGG TAAAGCACAT AGGTGACCCC 
CAAAGCTTGG GCCAGTCAGC TGTGTGGCTT CACCCCCAGG TCAACGTGAT CATCATCATC 
CTTCTCTTCT TCGTTATGAA GTTTTGGATG TCCATTGTAG CCACCACTAT GCCCATACCC 
TGTGGAGGCT TCATGCCTGT TTTTGTGCTA GGAGCTGCAT TTGGAAGGCT GGTAGGAGAG 
ATCATGGCCA TGCTATTCCC TGAGGGTATC TTATTTGATG ATATCATCTA TAAGATCTTA 
CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA GCTTTGACAG GGGCTGTCTC CCACACAGTC 
TCCACAGCCG TCATTTGCTT CGAATTAACC GGTCAGATTG CTCACATCCT GCCCATGATG 
GTGGCTGTTA TCTTGGCCAA CATGGTGGCT CAGAGTCTGC AGCCCTCCCT CTATGACAGC 
ATCATCCAGG TCAAGAAGCT TCCCTATCTG CCAGACCTTG GTTGGAACCA GCTCAGCAAA 
TTTACAATTT TTGTTGAGGA CATCATGGTA CGTGATGTGA AGTTCGTTTC AGCTTCTTGT 
ACATATGGGG AACTGAGAAA CCTACTCCAG GCCACCACAG TCAAGACTTT ACCATTGGTT 
GACTCCAAAG CCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505540.1
CDS	4165..6102
Translation	MERSQSQRHGGEQSWWGSAPQYQYMPFEHCTSYGLPSENGGLQHRPRKDMGPRHNAHPTQIYGHQKEQYSYKAQDGGMPKKMGSSSTMDSLDEDHYSKCQDCVHRLGRVLRRKLGEDWIFLVLLGLLMALVSWCMDYVSAKSLQAYKWTYAQMKPSLPLQYLAWVTFPLILILFSALFCQLISPQAVGSGIPEMKTILRGVVLKEYLTLKAFVAKVVALTAGLGSGIPVGKEGPFVHIASICAAVLSKFMSMFSGVYEQPYYYTDILTVGCAVGVGCCFGTPLGGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPAFAVIGICCGFLGAVFVYLHRQVMLGVRKHKCLSQFLAKHRLLYPGIVTFVIASLTFPPGMGQFMAGELMPREAISTLFDNNTWVKHIGDPQSLGQSAVWLHPQVNVIIIILLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPEGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKFTIFVEDIMVRDVKFVSASCTYGELRNLLQATTVKTLPLVDSKAV

Target ORF information:

RefSeq Version	XM_006505477.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505477.3

ORF Insert Sequence:

ATGGAGCGGT CCCAGTCCCA GCGGCATGGA GGGGAACAGA GCTGGTGGGG CAGTGCCCCC 
CAGTACCAGT ACATGCCCTT TGAACATTGT ACCAGCTACG GACTGCCCTC AGAGAATGGG 
GGCCTTCAGC ACCGGCCCCG AAAGGACATG GGTCCCAGGC ACAATGCCCA CCCAACACAG 
ATATATGGCC ATCAAAAAGA ACAATATTCA TATAAGGCAC AGGACGGGGG AATGCCCAAG 
AAGATGGGCT CCAGTTCTAC CATGGACAGC TTGGATGAGG ACCACTATTC TAAATGTCAA 
GACTGTGTCC ATCGCCTGGG ACGTGTGCTG AGAAGGAAGC TGGGGGAAGA TTGGATCTTT 
CTTGTGCTCC TGGGCCTCCT GATGGCTCTA GTCAGCTGGT GCATGGACTA TGTTAGTGCC 
AAGAGCCTTC AGGCCTACAA GTGGACCTAT GCCCAGATGA AGCCTAGCCT TCCTCTGCAG 
TACCTGGCCT GGGTCACCTT CCCACTTATC CTGATCCTCT TCAGCGCCCT CTTTTGCCAA 
CTCATCTCTC CCCAGGCTGT GGGCTCTGGA ATCCCTGAGA TGAAAACAAT TCTTCGTGGT 
GTTGTCTTGA AGGAATACCT CACACTCAAG GCCTTTGTAG CCAAGGTGGT AGCTTTGACA 
GCTGGACTGG GCAGTGGCAT CCCTGTGGGG AAAGAGGGTC CCTTTGTTCA CATCGCCAGC 
ATCTGTGCTG CTGTCCTCAG CAAGTTTATG TCCATGTTCT CTGGTGTCTA TGAGCAGCCA 
TACTATTACA CTGACATCCT GACAGTGGGC TGTGCCGTGG GGGTCGGTTG CTGTTTTGGA 
ACACCACTTG GAGGAGTGCT ATTTAGCATC GAGGTCACCT CTACCTACTT TGCTGTTCGG 
AATTACTGGC GAGGATTCTT TGCAGCCACA TTCAGTGCCT TTGTGTTCCG TGTCCTGGCC 
GTTTGGAACA AGGATGCTGT CACCATCACT GCTCTGTTCA GAACGAATTT CCGAATGGAT 
TTCCCCTTTG ACCTGAAGGA ACTCCCAGCT TTTGCTGTCA TTGGGATTTG CTGCGGGTTC 
TTGGGAGCTG TTTTCGTTTA TCTGCATCGC CAAGTCATGC TCGGTGTCCG AAAGCACAAG 
TGTCTCAGCC AATTTCTTGC TAAGCACCGC CTGCTATATC CTGGAATTGT TACCTTTGTC 
ATCGCCTCGC TCACATTTCC ACCAGGAATG GGTCAATTCA TGGCTGGAGA GCTGATGCCC 
CGTGAAGCTA TCAGTACCCT CTTTGACAAC AACACATGGG TAAAGCACAT AGGTGACCCC 
CAAAGCTTGG GCCAGTCAGC TGTGTGGCTT CACCCCCAGG TCAACGTGAT CATCATCATC 
CTTCTCTTCT TCGTTATGAA GTTTTGGATG TCCATTGTAG CCACCACTAT GCCCATACCC 
TGTGGAGGCT TCATGCCTGT TTTTGTGCTA GGAGCTGCAT TTGGAAGGCT GGTAGGAGAG 
ATCATGGCCA TGCTATTCCC TGAGGGTATC TTATTTGATG ATATCATCTA TAAGATCTTA 
CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA GCTTTGACAG GGGCTGTCTC CCACACAGTC 
TCCACAGCCG TCATTTGCTT CGAATTAACC GGTCAGATTG CTCACATCCT GCCCATGATG 
GTGGCTGTTA TCTTGGCCAA CATGGTGGCT CAGAGTCTGC AGCCCTCCCT CTATGACAGC 
ATCATCCAGG TCAAGAAGCT TCCCTATCTG CCAGACCTTG GTTGGAACCA GCTCAGCAAA 
TTTACAATTT TTGTTGAGGA CATCATGGTA CGTGATGTGA AGTTCGTTTC AGCTTCTTGT 
ACATATGGGG AACTGAGAAA CCTACTCCAG GCCACCACAG TCAAGACTTT ACCATTGGTT 
GACTCCAAAG CCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn1 ( NM_013491.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038519.1 Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), mRNA.

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CloneID	OMu15086
Clone ID Related Accession (Same CDS sequence)	NM_013491.2 , NM_013491.3
Accession Version	NM_013491.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-12
Organism	Mus musculus(house mouse)
Product	chloride channel protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK036735.1. On Apr 6, 2007 this sequence version replaced NM_013491.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036735.1, BC114336.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGGT CCCAGTCCCA GCGGCATGGA GGGGAACAGA GCTGGTGGGG CAGTGCCCCC 
CAGTACCAGT ACATGCCCTT TGAACATTGT ACCAGCTACG GACTGCCCTC AGAGAATGGG 
GGCCTTCAGC ACCGGCCCCG AAAGGACATG GGTCCCAGGC ACAATGCCCA CCCAACACAG 
ATATATGGCC ATCAAAAAGA ACAATATTCA TATAAGGCAC AGGACGGGGG AATGCCCAAG 
AAGATGGGCT CCAGTTCTAC CATGGACAGC TTGGATGAGG ACCACTATTC TAAATGTCAA 
GACTGTGTCC ATCGCCTGGG ACGTGTGCTG AGAAGGAAGC TGGGGGAAGA TTGGATCTTT 
CTTGTGCTCC TGGGCCTCCT GATGGCTCTA GTCAGCTGGT GCATGGACTA TGTTAGTGCC 
AAGAGCCTTC AGGCCTACAA GTGGACCTAT GCCCAGATGA AGCCTAGCCT TCCTCTGCAG 
TACCTGGCCT GGGTCACCTT CCCACTTATC CTGATCCTCT TCAGCGCCCT CTTTTGCCAA 
CTCATCTCTC CCCAGGCTGT GGGCTCTGGA ATCCCTGAGA TGAAAACAAT TCTTCGTGGT 
GTTGTCTTGA AGGAATACCT CACACTCAAG GCCTTTGTAG CCAAGGTGGT AGCTTTGACA 
GCTGGACTGG GCAGTGGCAT CCCTGTGGGG AAAGAGGGTC CCTTTGTTCA CATCGCCAGC 
ATCTGTGCTG CTGTCCTCAG CAAGTTTATG TCCATGTTCT CTGGTGTCTA TGAGCAGCCA 
TACTATTACA CTGACATCCT GACAGTGGGC TGTGCCGTGG GGGTCGGTTG CTGTTTTGGA 
ACACCACTTG GAGGAGTGCT ATTTAGCATC GAGGTCACCT CTACCTACTT TGCTGTTCGG 
AATTACTGGC GAGGATTCTT TGCAGCCACA TTCAGTGCCT TTGTGTTCCG TGTCCTGGCC 
GTTTGGAACA AGGATGCTGT CACCATCACT GCTCTGTTCA GAACGAATTT CCGAATGGAT 
TTCCCCTTTG ACCTGAAGGA ACTCCCAGCT TTTGCTGTCA TTGGGATTTG CTGCGGGTTC 
TTGGGAGCTG TTTTCGTTTA TCTGCATCGC CAAGTCATGC TCGGTGTCCG AAAGCACAAG 
TGTCTCAGCC AATTTCTTGC TAAGCACCGC CTGCTATATC CTGGAATTGT TACCTTTGTC 
ATCGCCTCGC TCACATTTCC ACCAGGAATG GGTCAATTCA TGGCTGGAGA GCTGATGCCC 
CGTGAAGCTA TCAGTACCCT CTTTGACAAC AACACATGGG TAAAGCACAT AGGTGACCCC 
CAAAGCTTGG GCCAGTCAGC TGTGTGGCTT CACCCCCAGG TCAACGTGAT CATCATCATC 
CTTCTCTTCT TCGTTATGAA GTTTTGGATG TCCATTGTAG CCACCACTAT GCCCATACCC 
TGTGGAGGCT TCATGCCTGT TTTTGTGCTA GGAGCTGCAT TTGGAAGGCT GGTAGGAGAG 
ATCATGGCCA TGCTATTCCC TGAGGGTATC TTATTTGATG ATATCATCTA TAAGATCTTA 
CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA GCTTTGACAG GGGCTGTCTC CCACACAGTC 
TCCACAGCCG TCATTTGCTT CGAATTAACC GGTCAGATTG CTCACATCCT GCCCATGATG 
GTGGCTGTTA TCTTGGCCAA CATGGTGGCT CAGAGTCTGC AGCCCTCCCT CTATGACAGC 
ATCATCCAGG TCAAGAAGCT TCCCTATCTG CCAGACCTTG GTTGGAACCA GCTCAGCAAA 
TTTACAATTT TTGTTGAGGA CATCATGGTA CGTGATGTGA AGTTCGTTTC AGCTTCTTGT 
ACATATGGGG AACTGAGAAA CCTACTCCAG GCCACCACAG TCAAGACTTT ACCATTGGTT 
GACTCCAAAG ATTCAATGAT CCTGCTGGGC TCTGTGGAAC GCTCAGAACT GCAGTCCCTC 
CTGCAGCGCC ACCTGTGTGC AGAGCGAAGG TTGAAGGCTG CCCAGGACAT GGCTCGAAAG 
TTATCAGAGC TGCCTTATAA TGGCAAGGCT CAGCTGGCTG GGGACTGGCA TCCTGGTGGT 
CGACCTGAGT CCTTTGCCTT CGTAGATGAA GATGAAGATG AGGACCTCTC CAGGAAGATG 
GAGCTTCCAC TGACTCCAGC TCCTCCCCCT CCTTCCCCTC CTCCTCCACC TTCCCAGTTT 
CCTATTGCTC CATCAAACCC TGAAGAACCT AATGGGCCAC TGCCCAGCCA TAAACAGCCA 
CCTGAAGCCT CAGACTCTGC AGATCAAAGA TCATCCACCT TCCAACGCCT GCTGCACTGC 
TTGCTAGGAA AAGCTCACTC CAAAAAGAAG AAAATAACAC AGGATTCAAC AGATTTAGTG 
GATAACATGT CACCTGAAGA GATTGAGGCC TGGGAGCGGG AGCAGCTGAG CCAGCCTGTG 
TGCTTTGATT GCTGCTGCAT CGACCAGTCT CCCTTCCAGC TGGTGGAGCA GACAACCCTG 
CACAAGACTC ATACACTCTT CTCACTTCTT GGCCTCCATC TTGCCTATGT GACCAGCATG 
GGGAAGCTCA GAGGTGTCTT GGCACTAGAA GAGCTACAGA AAGCTATTGA GGGCCACACC 
AAATCTGGGG TGCAGCTTCG CCCTCCACTT GCCAGCTTCC GGAATACAAC TTCAATCCGG 
AAGACTCCTG GGGGGCCACC CCCTCCTGCA GAGGGCTGGA ATGTACCTGA GGATGGAGAT 
GGGGCTCCTG GAAGAGAAGT CATGGTTCCT ACCATGCCAG AGACTCCTGT CCCACCACCA 
TCTCCAGAGG CCCCTTCCTG CCTGGCCCCA GCCAGAGCGG AGGGTGAGCT GGAGGAACTG 
GAGATGGTGG GGAGCCTAGA GCCTGAGGAG GAGCTGGCTG ACATCTTGCA TGGCCCCAGT 
CTGCGGTCCA CTGATGAGGA AGATGAGGAC GAGCTGATCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038519.1
CDS	83..3067
Translation	MERSQSQRHGGEQSWWGSAPQYQYMPFEHCTSYGLPSENGGLQHRPRKDMGPRHNAHPTQIYGHQKEQYSYKAQDGGMPKKMGSSSTMDSLDEDHYSKCQDCVHRLGRVLRRKLGEDWIFLVLLGLLMALVSWCMDYVSAKSLQAYKWTYAQMKPSLPLQYLAWVTFPLILILFSALFCQLISPQAVGSGIPEMKTILRGVVLKEYLTLKAFVAKVVALTAGLGSGIPVGKEGPFVHIASICAAVLSKFMSMFSGVYEQPYYYTDILTVGCAVGVGCCFGTPLGGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPAFAVIGICCGFLGAVFVYLHRQVMLGVRKHKCLSQFLAKHRLLYPGIVTFVIASLTFPPGMGQFMAGELMPREAISTLFDNNTWVKHIGDPQSLGQSAVWLHPQVNVIIIILLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPEGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKFTIFVEDIMVRDVKFVSASCTYGELRNLLQATTVKTLPLVDSKDSMILLGSVERSELQSLLQRHLCAERRLKAAQDMARKLSELPYNGKAQLAGDWHPGGRPESFAFVDEDEDEDLSRKMELPLTPAPPPPSPPPPPSQFPIAPSNPEEPNGPLPSHKQPPEASDSADQRSSTFQRLLHCLLGKAHSKKKKITQDSTDLVDNMSPEEIEAWEREQLSQPVCFDCCCIDQSPFQLVEQTTLHKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSIRKTPGGPPPPAEGWNVPEDGDGAPGREVMVPTMPETPVPPPSPEAPSCLAPARAEGELEELEMVGSLEPEEELADILHGPSLRSTDEEDEDELIL

Target ORF information:

RefSeq Version	NM_013491.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013491.2

ORF Insert Sequence:

ATGGAGCGGT CCCAGTCCCA GCGGCATGGA GGGGAACAGA GCTGGTGGGG CAGTGCCCCC 
CAGTACCAGT ACATGCCCTT TGAACATTGT ACCAGCTACG GACTGCCCTC AGAGAATGGG 
GGCCTTCAGC ACCGGCCCCG AAAGGACATG GGTCCCAGGC ACAATGCCCA CCCAACACAG 
ATATATGGCC ATCAAAAAGA ACAATATTCA TATAAGGCAC AGGACGGGGG AATGCCCAAG 
AAGATGGGCT CCAGTTCTAC CATGGACAGC TTGGATGAGG ACCACTATTC TAAATGTCAA 
GACTGTGTCC ATCGCCTGGG ACGTGTGCTG AGAAGGAAGC TGGGGGAAGA TTGGATCTTT 
CTTGTGCTCC TGGGCCTCCT GATGGCTCTA GTCAGCTGGT GCATGGACTA TGTTAGTGCC 
AAGAGCCTTC AGGCCTACAA GTGGACCTAT GCCCAGATGA AGCCTAGCCT TCCTCTGCAG 
TACCTGGCCT GGGTCACCTT CCCACTTATC CTGATCCTCT TCAGCGCCCT CTTTTGCCAA 
CTCATCTCTC CCCAGGCTGT GGGCTCTGGA ATCCCTGAGA TGAAAACAAT TCTTCGTGGT 
GTTGTCTTGA AGGAATACCT CACACTCAAG GCCTTTGTAG CCAAGGTGGT AGCTTTGACA 
GCTGGACTGG GCAGTGGCAT CCCTGTGGGG AAAGAGGGTC CCTTTGTTCA CATCGCCAGC 
ATCTGTGCTG CTGTCCTCAG CAAGTTTATG TCCATGTTCT CTGGTGTCTA TGAGCAGCCA 
TACTATTACA CTGACATCCT GACAGTGGGC TGTGCCGTGG GGGTCGGTTG CTGTTTTGGA 
ACACCACTTG GAGGAGTGCT ATTTAGCATC GAGGTCACCT CTACCTACTT TGCTGTTCGG 
AATTACTGGC GAGGATTCTT TGCAGCCACA TTCAGTGCCT TTGTGTTCCG TGTCCTGGCC 
GTTTGGAACA AGGATGCTGT CACCATCACT GCTCTGTTCA GAACGAATTT CCGAATGGAT 
TTCCCCTTTG ACCTGAAGGA ACTCCCAGCT TTTGCTGTCA TTGGGATTTG CTGCGGGTTC 
TTGGGAGCTG TTTTCGTTTA TCTGCATCGC CAAGTCATGC TCGGTGTCCG AAAGCACAAG 
TGTCTCAGCC AATTTCTTGC TAAGCACCGC CTGCTATATC CTGGAATTGT TACCTTTGTC 
ATCGCCTCGC TCACATTTCC ACCAGGAATG GGTCAATTCA TGGCTGGAGA GCTGATGCCC 
CGTGAAGCTA TCAGTACCCT CTTTGACAAC AACACATGGG TAAAGCACAT AGGTGACCCC 
CAAAGCTTGG GCCAGTCAGC TGTGTGGCTT CACCCCCAGG TCAACGTGAT CATCATCATC 
CTTCTCTTCT TCGTTATGAA GTTTTGGATG TCCATTGTAG CCACCACTAT GCCCATACCC 
TGTGGAGGCT TCATGCCTGT TTTTGTGCTA GGAGCTGCAT TTGGAAGGCT GGTAGGAGAG 
ATCATGGCCA TGCTATTCCC TGAGGGTATC TTATTTGATG ATATCATCTA TAAGATCTTA 
CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA GCTTTGACAG GGGCTGTCTC CCACACAGTC 
TCCACAGCCG TCATTTGCTT CGAATTAACC GGTCAGATTG CTCACATCCT GCCCATGATG 
GTGGCTGTTA TCTTGGCCAA CATGGTGGCT CAGAGTCTGC AGCCCTCCCT CTATGACAGC 
ATCATCCAGG TCAAGAAGCT TCCCTATCTG CCAGACCTTG GTTGGAACCA GCTCAGCAAA 
TTTACAATTT TTGTTGAGGA CATCATGGTA CGTGATGTGA AGTTCGTTTC AGCTTCTTGT 
ACATATGGGG AACTGAGAAA CCTACTCCAG GCCACCACAG TCAAGACTTT ACCATTGGTT 
GACTCCAAAG ATTCAATGAT CCTGCTGGGC TCTGTGGAAC GCTCAGAACT GCAGTCCCTC 
CTGCAGCGCC ACCTGTGTGC AGAGCGAAGG TTGAAGGCTG CCCAGGACAT GGCTCGAAAG 
TTATCAGAGC TGCCTTATAA TGGCAAGGCT CAGCTGGCTG GGGACTGGCA TCCTGGTGGT 
CGACCTGAGT CCTTTGCCTT CGTAGATGAA GATGAAGATG AGGACCTCTC CAGGAAGATG 
GAGCTTCCAC TGACTCCAGC TCCTCCCCCT CCTTCCCCTC CTCCTCCACC TTCCCAGTTT 
CCTATTGCTC CATCAAACCC TGAAGAACCT AATGGGCCAC TGCCCAGCCA TAAACAGCCA 
CCTGAAGCCT CAGACTCTGC AGATCAAAGA TCATCCACCT TCCAACGCCT GCTGCACTGC 
TTGCTAGGAA AAGCTCACTC CAAAAAGAAG AAAATAACAC AGGATTCAAC AGATTTAGTG 
GATAACATGT CACCTGAAGA GATTGAGGCC TGGGAGCGGG AGCAGCTGAG CCAGCCTGTG 
TGCTTTGATT GCTGCTGCAT CGACCAGTCT CCCTTCCAGC TGGTGGAGCA GACAACCCTG 
CACAAGACTC ATACACTCTT CTCACTTCTT GGCCTCCATC TTGCCTATGT GACCAGCATG 
GGGAAGCTCA GAGGTGTCTT GGCACTAGAA GAGCTACAGA AAGCTATTGA GGGCCACACC 
AAATCTGGGG TGCAGCTTCG CCCTCCACTT GCCAGCTTCC GGAATACAAC TTCAATCCGG 
AAGACTCCTG GGGGGCCACC CCCTCCTGCA GAGGGCTGGA ATGTACCTGA GGATGGAGAT 
GGGGCTCCTG GAAGAGAAGT CATGGTTCCT ACCATGCCAG AGACTCCTGT CCCACCACCA 
TCTCCAGAGG CCCCTTCCTG CCTGGCCCCA GCCAGAGCGG AGGGTGAGCT GGAGGAACTG 
GAGATGGTGG GGAGCCTAGA GCCTGAGGAG GAGCTGGCTG ACATCTTGCA TGGCCCCAGT 
CTGCGGTCCA CTGATGAGGA AGATGAGGAC GAGCTGATCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn1 ( NM_013491.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038519.1 Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15086
Clone ID Related Accession (Same CDS sequence)	NM_013491.2 , NM_013491.3
Accession Version	NM_013491.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	chloride channel protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK036735.1 and AC153915.5. On May 29, 2018 this sequence version replaced NM_013491.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036735.1, BC114336.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGT CCCAGTCCCA GCGGCATGGA GGGGAACAGA GCTGGTGGGG CAGTGCCCCC 
CAGTACCAGT ACATGCCCTT TGAACATTGT ACCAGCTACG GACTGCCCTC AGAGAATGGG 
GGCCTTCAGC ACCGGCCCCG AAAGGACATG GGTCCCAGGC ACAATGCCCA CCCAACACAG 
ATATATGGCC ATCAAAAAGA ACAATATTCA TATAAGGCAC AGGACGGGGG AATGCCCAAG 
AAGATGGGCT CCAGTTCTAC CATGGACAGC TTGGATGAGG ACCACTATTC TAAATGTCAA 
GACTGTGTCC ATCGCCTGGG ACGTGTGCTG AGAAGGAAGC TGGGGGAAGA TTGGATCTTT 
CTTGTGCTCC TGGGCCTCCT GATGGCTCTA GTCAGCTGGT GCATGGACTA TGTTAGTGCC 
AAGAGCCTTC AGGCCTACAA GTGGACCTAT GCCCAGATGA AGCCTAGCCT TCCTCTGCAG 
TACCTGGCCT GGGTCACCTT CCCACTTATC CTGATCCTCT TCAGCGCCCT CTTTTGCCAA 
CTCATCTCTC CCCAGGCTGT GGGCTCTGGA ATCCCTGAGA TGAAAACAAT TCTTCGTGGT 
GTTGTCTTGA AGGAATACCT CACACTCAAG GCCTTTGTAG CCAAGGTGGT AGCTTTGACA 
GCTGGACTGG GCAGTGGCAT CCCTGTGGGG AAAGAGGGTC CCTTTGTTCA CATCGCCAGC 
ATCTGTGCTG CTGTCCTCAG CAAGTTTATG TCCATGTTCT CTGGTGTCTA TGAGCAGCCA 
TACTATTACA CTGACATCCT GACAGTGGGC TGTGCCGTGG GGGTCGGTTG CTGTTTTGGA 
ACACCACTTG GAGGAGTGCT ATTTAGCATC GAGGTCACCT CTACCTACTT TGCTGTTCGG 
AATTACTGGC GAGGATTCTT TGCAGCCACA TTCAGTGCCT TTGTGTTCCG TGTCCTGGCC 
GTTTGGAACA AGGATGCTGT CACCATCACT GCTCTGTTCA GAACGAATTT CCGAATGGAT 
TTCCCCTTTG ACCTGAAGGA ACTCCCAGCT TTTGCTGTCA TTGGGATTTG CTGCGGGTTC 
TTGGGAGCTG TTTTCGTTTA TCTGCATCGC CAAGTCATGC TCGGTGTCCG AAAGCACAAG 
TGTCTCAGCC AATTTCTTGC TAAGCACCGC CTGCTATATC CTGGAATTGT TACCTTTGTC 
ATCGCCTCGC TCACATTTCC ACCAGGAATG GGTCAATTCA TGGCTGGAGA GCTGATGCCC 
CGTGAAGCTA TCAGTACCCT CTTTGACAAC AACACATGGG TAAAGCACAT AGGTGACCCC 
CAAAGCTTGG GCCAGTCAGC TGTGTGGCTT CACCCCCAGG TCAACGTGAT CATCATCATC 
CTTCTCTTCT TCGTTATGAA GTTTTGGATG TCCATTGTAG CCACCACTAT GCCCATACCC 
TGTGGAGGCT TCATGCCTGT TTTTGTGCTA GGAGCTGCAT TTGGAAGGCT GGTAGGAGAG 
ATCATGGCCA TGCTATTCCC TGAGGGTATC TTATTTGATG ATATCATCTA TAAGATCTTA 
CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA GCTTTGACAG GGGCTGTCTC CCACACAGTC 
TCCACAGCCG TCATTTGCTT CGAATTAACC GGTCAGATTG CTCACATCCT GCCCATGATG 
GTGGCTGTTA TCTTGGCCAA CATGGTGGCT CAGAGTCTGC AGCCCTCCCT CTATGACAGC 
ATCATCCAGG TCAAGAAGCT TCCCTATCTG CCAGACCTTG GTTGGAACCA GCTCAGCAAA 
TTTACAATTT TTGTTGAGGA CATCATGGTA CGTGATGTGA AGTTCGTTTC AGCTTCTTGT 
ACATATGGGG AACTGAGAAA CCTACTCCAG GCCACCACAG TCAAGACTTT ACCATTGGTT 
GACTCCAAAG ATTCAATGAT CCTGCTGGGC TCTGTGGAAC GCTCAGAACT GCAGTCCCTC 
CTGCAGCGCC ACCTGTGTGC AGAGCGAAGG TTGAAGGCTG CCCAGGACAT GGCTCGAAAG 
TTATCAGAGC TGCCTTATAA TGGCAAGGCT CAGCTGGCTG GGGACTGGCA TCCTGGTGGT 
CGACCTGAGT CCTTTGCCTT CGTAGATGAA GATGAAGATG AGGACCTCTC CAGGAAGATG 
GAGCTTCCAC TGACTCCAGC TCCTCCCCCT CCTTCCCCTC CTCCTCCACC TTCCCAGTTT 
CCTATTGCTC CATCAAACCC TGAAGAACCT AATGGGCCAC TGCCCAGCCA TAAACAGCCA 
CCTGAAGCCT CAGACTCTGC AGATCAAAGA TCATCCACCT TCCAACGCCT GCTGCACTGC 
TTGCTAGGAA AAGCTCACTC CAAAAAGAAG AAAATAACAC AGGATTCAAC AGATTTAGTG 
GATAACATGT CACCTGAAGA GATTGAGGCC TGGGAGCGGG AGCAGCTGAG CCAGCCTGTG 
TGCTTTGATT GCTGCTGCAT CGACCAGTCT CCCTTCCAGC TGGTGGAGCA GACAACCCTG 
CACAAGACTC ATACACTCTT CTCACTTCTT GGCCTCCATC TTGCCTATGT GACCAGCATG 
GGGAAGCTCA GAGGTGTCTT GGCACTAGAA GAGCTACAGA AAGCTATTGA GGGCCACACC 
AAATCTGGGG TGCAGCTTCG CCCTCCACTT GCCAGCTTCC GGAATACAAC TTCAATCCGG 
AAGACTCCTG GGGGGCCACC CCCTCCTGCA GAGGGCTGGA ATGTACCTGA GGATGGAGAT 
GGGGCTCCTG GAAGAGAAGT CATGGTTCCT ACCATGCCAG AGACTCCTGT CCCACCACCA 
TCTCCAGAGG CCCCTTCCTG CCTGGCCCCA GCCAGAGCGG AGGGTGAGCT GGAGGAACTG 
GAGATGGTGG GGAGCCTAGA GCCTGAGGAG GAGCTGGCTG ACATCTTGCA TGGCCCCAGT 
CTGCGGTCCA CTGATGAGGA AGATGAGGAC GAGCTGATCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038519.1
CDS	83..3067
Translation	MERSQSQRHGGEQSWWGSAPQYQYMPFEHCTSYGLPSENGGLQHRPRKDMGPRHNAHPTQIYGHQKEQYSYKAQDGGMPKKMGSSSTMDSLDEDHYSKCQDCVHRLGRVLRRKLGEDWIFLVLLGLLMALVSWCMDYVSAKSLQAYKWTYAQMKPSLPLQYLAWVTFPLILILFSALFCQLISPQAVGSGIPEMKTILRGVVLKEYLTLKAFVAKVVALTAGLGSGIPVGKEGPFVHIASICAAVLSKFMSMFSGVYEQPYYYTDILTVGCAVGVGCCFGTPLGGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPAFAVIGICCGFLGAVFVYLHRQVMLGVRKHKCLSQFLAKHRLLYPGIVTFVIASLTFPPGMGQFMAGELMPREAISTLFDNNTWVKHIGDPQSLGQSAVWLHPQVNVIIIILLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPEGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKFTIFVEDIMVRDVKFVSASCTYGELRNLLQATTVKTLPLVDSKDSMILLGSVERSELQSLLQRHLCAERRLKAAQDMARKLSELPYNGKAQLAGDWHPGGRPESFAFVDEDEDEDLSRKMELPLTPAPPPPSPPPPPSQFPIAPSNPEEPNGPLPSHKQPPEASDSADQRSSTFQRLLHCLLGKAHSKKKKITQDSTDLVDNMSPEEIEAWEREQLSQPVCFDCCCIDQSPFQLVEQTTLHKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSIRKTPGGPPPPAEGWNVPEDGDGAPGREVMVPTMPETPVPPPSPEAPSCLAPARAEGELEELEMVGSLEPEEELADILHGPSLRSTDEEDEDELIL

Target ORF information:

RefSeq Version	NM_013491.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013491.3

ORF Insert Sequence:

ATGGAGCGGT CCCAGTCCCA GCGGCATGGA GGGGAACAGA GCTGGTGGGG CAGTGCCCCC 
CAGTACCAGT ACATGCCCTT TGAACATTGT ACCAGCTACG GACTGCCCTC AGAGAATGGG 
GGCCTTCAGC ACCGGCCCCG AAAGGACATG GGTCCCAGGC ACAATGCCCA CCCAACACAG 
ATATATGGCC ATCAAAAAGA ACAATATTCA TATAAGGCAC AGGACGGGGG AATGCCCAAG 
AAGATGGGCT CCAGTTCTAC CATGGACAGC TTGGATGAGG ACCACTATTC TAAATGTCAA 
GACTGTGTCC ATCGCCTGGG ACGTGTGCTG AGAAGGAAGC TGGGGGAAGA TTGGATCTTT 
CTTGTGCTCC TGGGCCTCCT GATGGCTCTA GTCAGCTGGT GCATGGACTA TGTTAGTGCC 
AAGAGCCTTC AGGCCTACAA GTGGACCTAT GCCCAGATGA AGCCTAGCCT TCCTCTGCAG 
TACCTGGCCT GGGTCACCTT CCCACTTATC CTGATCCTCT TCAGCGCCCT CTTTTGCCAA 
CTCATCTCTC CCCAGGCTGT GGGCTCTGGA ATCCCTGAGA TGAAAACAAT TCTTCGTGGT 
GTTGTCTTGA AGGAATACCT CACACTCAAG GCCTTTGTAG CCAAGGTGGT AGCTTTGACA 
GCTGGACTGG GCAGTGGCAT CCCTGTGGGG AAAGAGGGTC CCTTTGTTCA CATCGCCAGC 
ATCTGTGCTG CTGTCCTCAG CAAGTTTATG TCCATGTTCT CTGGTGTCTA TGAGCAGCCA 
TACTATTACA CTGACATCCT GACAGTGGGC TGTGCCGTGG GGGTCGGTTG CTGTTTTGGA 
ACACCACTTG GAGGAGTGCT ATTTAGCATC GAGGTCACCT CTACCTACTT TGCTGTTCGG 
AATTACTGGC GAGGATTCTT TGCAGCCACA TTCAGTGCCT TTGTGTTCCG TGTCCTGGCC 
GTTTGGAACA AGGATGCTGT CACCATCACT GCTCTGTTCA GAACGAATTT CCGAATGGAT 
TTCCCCTTTG ACCTGAAGGA ACTCCCAGCT TTTGCTGTCA TTGGGATTTG CTGCGGGTTC 
TTGGGAGCTG TTTTCGTTTA TCTGCATCGC CAAGTCATGC TCGGTGTCCG AAAGCACAAG 
TGTCTCAGCC AATTTCTTGC TAAGCACCGC CTGCTATATC CTGGAATTGT TACCTTTGTC 
ATCGCCTCGC TCACATTTCC ACCAGGAATG GGTCAATTCA TGGCTGGAGA GCTGATGCCC 
CGTGAAGCTA TCAGTACCCT CTTTGACAAC AACACATGGG TAAAGCACAT AGGTGACCCC 
CAAAGCTTGG GCCAGTCAGC TGTGTGGCTT CACCCCCAGG TCAACGTGAT CATCATCATC 
CTTCTCTTCT TCGTTATGAA GTTTTGGATG TCCATTGTAG CCACCACTAT GCCCATACCC 
TGTGGAGGCT TCATGCCTGT TTTTGTGCTA GGAGCTGCAT TTGGAAGGCT GGTAGGAGAG 
ATCATGGCCA TGCTATTCCC TGAGGGTATC TTATTTGATG ATATCATCTA TAAGATCTTA 
CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA GCTTTGACAG GGGCTGTCTC CCACACAGTC 
TCCACAGCCG TCATTTGCTT CGAATTAACC GGTCAGATTG CTCACATCCT GCCCATGATG 
GTGGCTGTTA TCTTGGCCAA CATGGTGGCT CAGAGTCTGC AGCCCTCCCT CTATGACAGC 
ATCATCCAGG TCAAGAAGCT TCCCTATCTG CCAGACCTTG GTTGGAACCA GCTCAGCAAA 
TTTACAATTT TTGTTGAGGA CATCATGGTA CGTGATGTGA AGTTCGTTTC AGCTTCTTGT 
ACATATGGGG AACTGAGAAA CCTACTCCAG GCCACCACAG TCAAGACTTT ACCATTGGTT 
GACTCCAAAG ATTCAATGAT CCTGCTGGGC TCTGTGGAAC GCTCAGAACT GCAGTCCCTC 
CTGCAGCGCC ACCTGTGTGC AGAGCGAAGG TTGAAGGCTG CCCAGGACAT GGCTCGAAAG 
TTATCAGAGC TGCCTTATAA TGGCAAGGCT CAGCTGGCTG GGGACTGGCA TCCTGGTGGT 
CGACCTGAGT CCTTTGCCTT CGTAGATGAA GATGAAGATG AGGACCTCTC CAGGAAGATG 
GAGCTTCCAC TGACTCCAGC TCCTCCCCCT CCTTCCCCTC CTCCTCCACC TTCCCAGTTT 
CCTATTGCTC CATCAAACCC TGAAGAACCT AATGGGCCAC TGCCCAGCCA TAAACAGCCA 
CCTGAAGCCT CAGACTCTGC AGATCAAAGA TCATCCACCT TCCAACGCCT GCTGCACTGC 
TTGCTAGGAA AAGCTCACTC CAAAAAGAAG AAAATAACAC AGGATTCAAC AGATTTAGTG 
GATAACATGT CACCTGAAGA GATTGAGGCC TGGGAGCGGG AGCAGCTGAG CCAGCCTGTG 
TGCTTTGATT GCTGCTGCAT CGACCAGTCT CCCTTCCAGC TGGTGGAGCA GACAACCCTG 
CACAAGACTC ATACACTCTT CTCACTTCTT GGCCTCCATC TTGCCTATGT GACCAGCATG 
GGGAAGCTCA GAGGTGTCTT GGCACTAGAA GAGCTACAGA AAGCTATTGA GGGCCACACC 
AAATCTGGGG TGCAGCTTCG CCCTCCACTT GCCAGCTTCC GGAATACAAC TTCAATCCGG 
AAGACTCCTG GGGGGCCACC CCCTCCTGCA GAGGGCTGGA ATGTACCTGA GGATGGAGAT 
GGGGCTCCTG GAAGAGAAGT CATGGTTCCT ACCATGCCAG AGACTCCTGT CCCACCACCA 
TCTCCAGAGG CCCCTTCCTG CCTGGCCCCA GCCAGAGCGG AGGGTGAGCT GGAGGAACTG 
GAGATGGTGG GGAGCCTAGA GCCTGAGGAG GAGCTGGCTG ACATCTTGCA TGGCCCCAGT 
CTGCGGTCCA CTGATGAGGA AGATGAGGAC GAGCTGATCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn1 ( NM_001363712.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350641.1 Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant 2, mRNA.

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CloneID	OMu67375
Clone ID Related Accession (Same CDS sequence)	XM_011241199.1 , NM_001363712.1
Accession Version	NM_001363712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Mus musculus(house mouse)
Product	chloride channel protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153915.5. On May 30, 2018 this sequence version replaced XM_011241199.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY046404.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849390 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGACCGT GCCTGGGCAG CTTGATCTCC TGGTGCCAGC CTGTGCAGTG GGCGTGGGAT 
GCTACTTTGC AGCCCCTGAT GGAGCAGCCA TACTATTACA CTGACATCCT GACAGTGGGC 
TGTGCCGTGG GGGTCGGTTG CTGTTTTGGA ACACCACTTG GAGGAGTGCT ATTTAGCATC 
GAGGTCACCT CTACCTACTT TGCTGTTCGG AATTACTGGC GAGGATTCTT TGCAGCCACA 
TTCAGTGCCT TTGTGTTCCG TGTCCTGGCC GTTTGGAACA AGGATGCTGT CACCATCACT 
GCTCTGTTCA GAACGAATTT CCGAATGGAT TTCCCCTTTG ACCTGAAGGA ACTCCCAGCT 
TTTGCTGTCA TTGGGATTTG CTGCGGGTTC TTGGGAGCTG TTTTCGTTTA TCTGCATCGC 
CAAGTCATGC TCGGTGTCCG AAAGCACAAG TGTCTCAGCC AATTTCTTGC TAAGCACCGC 
CTGCTATATC CTGGAATTGT TACCTTTGTC ATCGCCTCGC TCACATTTCC ACCAGGAATG 
GGTCAATTCA TGGCTGGAGA GCTGATGCCC CGTGAAGCTA TCAGTACCCT CTTTGACAAC 
AACACATGGG TAAAGCACAT AGGTGACCCC CAAAGCTTGG GCCAGTCAGC TGTGTGGCTT 
CACCCCCAGG TCAACGTGAT CATCATCATC CTTCTCTTCT TCGTTATGAA GTTTTGGATG 
TCCATTGTAG CCACCACTAT GCCCATACCC TGTGGAGGCT TCATGCCTGT TTTTGTGCTA 
GGAGCTGCAT TTGGAAGGCT GGTAGGAGAG ATCATGGCCA TGCTATTCCC TGAGGGTATC 
TTATTTGATG ATATCATCTA TAAGATCTTA CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA 
GCTTTGACAG GGGCTGTCTC CCACACAGTC TCCACAGCCG TCATTTGCTT CGAATTAACC 
GGTCAGATTG CTCACATCCT GCCCATGATG GTGGCTGTTA TCTTGGCCAA CATGGTGGCT 
CAGAGTCTGC AGCCCTCCCT CTATGACAGC ATCATCCAGG TCAAGAAGCT TCCCTATCTG 
CCAGACCTTG GTTGGAACCA GCTCAGCAAA TTTACAATTT TTGTTGAGGA CATCATGGTA 
CGTGATGTGA AGTTCGTTTC AGCTTCTTGT ACATATGGGG AACTGAGAAA CCTACTCCAG 
GCCACCACAG TCAAGACTTT ACCATTGGTT GACTCCAAAG ATTCAATGAT CCTGCTGGGC 
TCTGTGGAAC GCTCAGAACT GCAGTCCCTC CTGCAGCGCC ACCTGTGTGC AGAGCGAAGG 
TTGAAGGCTG CCCAGGACAT GGCTCGAAAG TTATCAGAGC TGCCTTATAA TGGCAAGGCT 
CAGCTGGCTG GGGACTGGCA TCCTGGTGGT CGACCTGAGT CCTTTGCCTT CGTAGATGAA 
GATGAAGATG AGGACCTCTC CAGGAAGATG GAGCTTCCAC TGACTCCAGC TCCTCCCCCT 
CCTTCCCCTC CTCCTCCACC TTCCCAGTTT CCTATTGCTC CATCAAACCC TGAAGAACCT 
AATGGGCCAC TGCCCAGCCA TAAACAGCCA CCTGAAGCCT CAGACTCTGC AGATCAAAGA 
TCATCCACCT TCCAACGCCT GCTGCACTGC TTGCTAGGAA AAGCTCACTC CAAAAAGAAG 
AAAATAACAC AGGATTCAAC AGATTTAGTG GATAACATGT CACCTGAAGA GATTGAGGCC 
TGGGAGCGGG AGCAGCTGAG CCAGCCTGTG TGCTTTGATT GCTGCTGCAT CGACCAGTCT 
CCCTTCCAGC TGGTGGAGCA GACAACCCTG CACAAGACTC ATACACTCTT CTCACTTCTT 
GGCCTCCATC TTGCCTATGT GACCAGCATG GGGAAGCTCA GAGGTGTCTT GGCACTAGAA 
GAGCTACAGA AAGCTATTGA GGGCCACACC AAATCTGGGG TGCAGCTTCG CCCTCCACTT 
GCCAGCTTCC GGAATACAAC TTCAATCCGG AAGACTCCTG GGGGGCCACC CCCTCCTGCA 
GAGGGCTGGA ATGTACCTGA GGATGGAGAT GGGGCTCCTG GAAGAGAAGT CATGGTTCCT 
ACCATGCCAG AGACTCCTGT CCCACCACCA TCTCCAGAGG CCCCTTCCTG CCTGGCCCCA 
GCCAGAGCGG AGGGTGAGCT GGAGGAACTG GAGATGGTGG GGAGCCTAGA GCCTGAGGAG 
GAGCTGGCTG ACATCTTGCA TGGCCCCAGT CTGCGGTCCA CTGATGAGGA AGATGAGGAC 
GAGCTGATCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350641.1
CDS	852..3146
Translation	MRPCLGSLISWCQPVQWAWDATLQPLMEQPYYYTDILTVGCAVGVGCCFGTPLGGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPAFAVIGICCGFLGAVFVYLHRQVMLGVRKHKCLSQFLAKHRLLYPGIVTFVIASLTFPPGMGQFMAGELMPREAISTLFDNNTWVKHIGDPQSLGQSAVWLHPQVNVIIIILLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPEGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKFTIFVEDIMVRDVKFVSASCTYGELRNLLQATTVKTLPLVDSKDSMILLGSVERSELQSLLQRHLCAERRLKAAQDMARKLSELPYNGKAQLAGDWHPGGRPESFAFVDEDEDEDLSRKMELPLTPAPPPPSPPPPPSQFPIAPSNPEEPNGPLPSHKQPPEASDSADQRSSTFQRLLHCLLGKAHSKKKKITQDSTDLVDNMSPEEIEAWEREQLSQPVCFDCCCIDQSPFQLVEQTTLHKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSIRKTPGGPPPPAEGWNVPEDGDGAPGREVMVPTMPETPVPPPSPEAPSCLAPARAEGELEELEMVGSLEPEEELADILHGPSLRSTDEEDEDELIL

Target ORF information:

RefSeq Version	NM_001363712.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363712.1

ORF Insert Sequence:

ATGAGACCGT GCCTGGGCAG CTTGATCTCC TGGTGCCAGC CTGTGCAGTG GGCGTGGGAT 
GCTACTTTGC AGCCCCTGAT GGAGCAGCCA TACTATTACA CTGACATCCT GACAGTGGGC 
TGTGCCGTGG GGGTCGGTTG CTGTTTTGGA ACACCACTTG GAGGAGTGCT ATTTAGCATC 
GAGGTCACCT CTACCTACTT TGCTGTTCGG AATTACTGGC GAGGATTCTT TGCAGCCACA 
TTCAGTGCCT TTGTGTTCCG TGTCCTGGCC GTTTGGAACA AGGATGCTGT CACCATCACT 
GCTCTGTTCA GAACGAATTT CCGAATGGAT TTCCCCTTTG ACCTGAAGGA ACTCCCAGCT 
TTTGCTGTCA TTGGGATTTG CTGCGGGTTC TTGGGAGCTG TTTTCGTTTA TCTGCATCGC 
CAAGTCATGC TCGGTGTCCG AAAGCACAAG TGTCTCAGCC AATTTCTTGC TAAGCACCGC 
CTGCTATATC CTGGAATTGT TACCTTTGTC ATCGCCTCGC TCACATTTCC ACCAGGAATG 
GGTCAATTCA TGGCTGGAGA GCTGATGCCC CGTGAAGCTA TCAGTACCCT CTTTGACAAC 
AACACATGGG TAAAGCACAT AGGTGACCCC CAAAGCTTGG GCCAGTCAGC TGTGTGGCTT 
CACCCCCAGG TCAACGTGAT CATCATCATC CTTCTCTTCT TCGTTATGAA GTTTTGGATG 
TCCATTGTAG CCACCACTAT GCCCATACCC TGTGGAGGCT TCATGCCTGT TTTTGTGCTA 
GGAGCTGCAT TTGGAAGGCT GGTAGGAGAG ATCATGGCCA TGCTATTCCC TGAGGGTATC 
TTATTTGATG ATATCATCTA TAAGATCTTA CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA 
GCTTTGACAG GGGCTGTCTC CCACACAGTC TCCACAGCCG TCATTTGCTT CGAATTAACC 
GGTCAGATTG CTCACATCCT GCCCATGATG GTGGCTGTTA TCTTGGCCAA CATGGTGGCT 
CAGAGTCTGC AGCCCTCCCT CTATGACAGC ATCATCCAGG TCAAGAAGCT TCCCTATCTG 
CCAGACCTTG GTTGGAACCA GCTCAGCAAA TTTACAATTT TTGTTGAGGA CATCATGGTA 
CGTGATGTGA AGTTCGTTTC AGCTTCTTGT ACATATGGGG AACTGAGAAA CCTACTCCAG 
GCCACCACAG TCAAGACTTT ACCATTGGTT GACTCCAAAG ATTCAATGAT CCTGCTGGGC 
TCTGTGGAAC GCTCAGAACT GCAGTCCCTC CTGCAGCGCC ACCTGTGTGC AGAGCGAAGG 
TTGAAGGCTG CCCAGGACAT GGCTCGAAAG TTATCAGAGC TGCCTTATAA TGGCAAGGCT 
CAGCTGGCTG GGGACTGGCA TCCTGGTGGT CGACCTGAGT CCTTTGCCTT CGTAGATGAA 
GATGAAGATG AGGACCTCTC CAGGAAGATG GAGCTTCCAC TGACTCCAGC TCCTCCCCCT 
CCTTCCCCTC CTCCTCCACC TTCCCAGTTT CCTATTGCTC CATCAAACCC TGAAGAACCT 
AATGGGCCAC TGCCCAGCCA TAAACAGCCA CCTGAAGCCT CAGACTCTGC AGATCAAAGA 
TCATCCACCT TCCAACGCCT GCTGCACTGC TTGCTAGGAA AAGCTCACTC CAAAAAGAAG 
AAAATAACAC AGGATTCAAC AGATTTAGTG GATAACATGT CACCTGAAGA GATTGAGGCC 
TGGGAGCGGG AGCAGCTGAG CCAGCCTGTG TGCTTTGATT GCTGCTGCAT CGACCAGTCT 
CCCTTCCAGC TGGTGGAGCA GACAACCCTG CACAAGACTC ATACACTCTT CTCACTTCTT 
GGCCTCCATC TTGCCTATGT GACCAGCATG GGGAAGCTCA GAGGTGTCTT GGCACTAGAA 
GAGCTACAGA AAGCTATTGA GGGCCACACC AAATCTGGGG TGCAGCTTCG CCCTCCACTT 
GCCAGCTTCC GGAATACAAC TTCAATCCGG AAGACTCCTG GGGGGCCACC CCCTCCTGCA 
GAGGGCTGGA ATGTACCTGA GGATGGAGAT GGGGCTCCTG GAAGAGAAGT CATGGTTCCT 
ACCATGCCAG AGACTCCTGT CCCACCACCA TCTCCAGAGG CCCCTTCCTG CCTGGCCCCA 
GCCAGAGCGG AGGGTGAGCT GGAGGAACTG GAGATGGTGG GGAGCCTAGA GCCTGAGGAG 
GAGCTGGCTG ACATCTTGCA TGGCCCCAGT CTGCGGTCCA CTGATGAGGA AGATGAGGAC 
GAGCTGATCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn1 ( XM_011241199.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239501.1 Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant X1, mRNA.

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CloneID	OMu67375
Clone ID Related Accession (Same CDS sequence)	XM_011241199.1 , NM_001363712.1
Accession Version	XM_011241199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chloride channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCGT GCCTGGGCAG CTTGATCTCC TGGTGCCAGC CTGTGCAGTG GGCGTGGGAT 
GCTACTTTGC AGCCCCTGAT GGAGCAGCCA TACTATTACA CTGACATCCT GACAGTGGGC 
TGTGCCGTGG GGGTCGGTTG CTGTTTTGGA ACACCACTTG GAGGAGTGCT ATTTAGCATC 
GAGGTCACCT CTACCTACTT TGCTGTTCGG AATTACTGGC GAGGATTCTT TGCAGCCACA 
TTCAGTGCCT TTGTGTTCCG TGTCCTGGCC GTTTGGAACA AGGATGCTGT CACCATCACT 
GCTCTGTTCA GAACGAATTT CCGAATGGAT TTCCCCTTTG ACCTGAAGGA ACTCCCAGCT 
TTTGCTGTCA TTGGGATTTG CTGCGGGTTC TTGGGAGCTG TTTTCGTTTA TCTGCATCGC 
CAAGTCATGC TCGGTGTCCG AAAGCACAAG TGTCTCAGCC AATTTCTTGC TAAGCACCGC 
CTGCTATATC CTGGAATTGT TACCTTTGTC ATCGCCTCGC TCACATTTCC ACCAGGAATG 
GGTCAATTCA TGGCTGGAGA GCTGATGCCC CGTGAAGCTA TCAGTACCCT CTTTGACAAC 
AACACATGGG TAAAGCACAT AGGTGACCCC CAAAGCTTGG GCCAGTCAGC TGTGTGGCTT 
CACCCCCAGG TCAACGTGAT CATCATCATC CTTCTCTTCT TCGTTATGAA GTTTTGGATG 
TCCATTGTAG CCACCACTAT GCCCATACCC TGTGGAGGCT TCATGCCTGT TTTTGTGCTA 
GGAGCTGCAT TTGGAAGGCT GGTAGGAGAG ATCATGGCCA TGCTATTCCC TGAGGGTATC 
TTATTTGATG ATATCATCTA TAAGATCTTA CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA 
GCTTTGACAG GGGCTGTCTC CCACACAGTC TCCACAGCCG TCATTTGCTT CGAATTAACC 
GGTCAGATTG CTCACATCCT GCCCATGATG GTGGCTGTTA TCTTGGCCAA CATGGTGGCT 
CAGAGTCTGC AGCCCTCCCT CTATGACAGC ATCATCCAGG TCAAGAAGCT TCCCTATCTG 
CCAGACCTTG GTTGGAACCA GCTCAGCAAA TTTACAATTT TTGTTGAGGA CATCATGGTA 
CGTGATGTGA AGTTCGTTTC AGCTTCTTGT ACATATGGGG AACTGAGAAA CCTACTCCAG 
GCCACCACAG TCAAGACTTT ACCATTGGTT GACTCCAAAG ATTCAATGAT CCTGCTGGGC 
TCTGTGGAAC GCTCAGAACT GCAGTCCCTC CTGCAGCGCC ACCTGTGTGC AGAGCGAAGG 
TTGAAGGCTG CCCAGGACAT GGCTCGAAAG TTATCAGAGC TGCCTTATAA TGGCAAGGCT 
CAGCTGGCTG GGGACTGGCA TCCTGGTGGT CGACCTGAGT CCTTTGCCTT CGTAGATGAA 
GATGAAGATG AGGACCTCTC CAGGAAGATG GAGCTTCCAC TGACTCCAGC TCCTCCCCCT 
CCTTCCCCTC CTCCTCCACC TTCCCAGTTT CCTATTGCTC CATCAAACCC TGAAGAACCT 
AATGGGCCAC TGCCCAGCCA TAAACAGCCA CCTGAAGCCT CAGACTCTGC AGATCAAAGA 
TCATCCACCT TCCAACGCCT GCTGCACTGC TTGCTAGGAA AAGCTCACTC CAAAAAGAAG 
AAAATAACAC AGGATTCAAC AGATTTAGTG GATAACATGT CACCTGAAGA GATTGAGGCC 
TGGGAGCGGG AGCAGCTGAG CCAGCCTGTG TGCTTTGATT GCTGCTGCAT CGACCAGTCT 
CCCTTCCAGC TGGTGGAGCA GACAACCCTG CACAAGACTC ATACACTCTT CTCACTTCTT 
GGCCTCCATC TTGCCTATGT GACCAGCATG GGGAAGCTCA GAGGTGTCTT GGCACTAGAA 
GAGCTACAGA AAGCTATTGA GGGCCACACC AAATCTGGGG TGCAGCTTCG CCCTCCACTT 
GCCAGCTTCC GGAATACAAC TTCAATCCGG AAGACTCCTG GGGGGCCACC CCCTCCTGCA 
GAGGGCTGGA ATGTACCTGA GGATGGAGAT GGGGCTCCTG GAAGAGAAGT CATGGTTCCT 
ACCATGCCAG AGACTCCTGT CCCACCACCA TCTCCAGAGG CCCCTTCCTG CCTGGCCCCA 
GCCAGAGCGG AGGGTGAGCT GGAGGAACTG GAGATGGTGG GGAGCCTAGA GCCTGAGGAG 
GAGCTGGCTG ACATCTTGCA TGGCCCCAGT CTGCGGTCCA CTGATGAGGA AGATGAGGAC 
GAGCTGATCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239501.1
CDS	70..2364
Translation	MRPCLGSLISWCQPVQWAWDATLQPLMEQPYYYTDILTVGCAVGVGCCFGTPLGGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPAFAVIGICCGFLGAVFVYLHRQVMLGVRKHKCLSQFLAKHRLLYPGIVTFVIASLTFPPGMGQFMAGELMPREAISTLFDNNTWVKHIGDPQSLGQSAVWLHPQVNVIIIILLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPEGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKFTIFVEDIMVRDVKFVSASCTYGELRNLLQATTVKTLPLVDSKDSMILLGSVERSELQSLLQRHLCAERRLKAAQDMARKLSELPYNGKAQLAGDWHPGGRPESFAFVDEDEDEDLSRKMELPLTPAPPPPSPPPPPSQFPIAPSNPEEPNGPLPSHKQPPEASDSADQRSSTFQRLLHCLLGKAHSKKKKITQDSTDLVDNMSPEEIEAWEREQLSQPVCFDCCCIDQSPFQLVEQTTLHKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSIRKTPGGPPPPAEGWNVPEDGDGAPGREVMVPTMPETPVPPPSPEAPSCLAPARAEGELEELEMVGSLEPEEELADILHGPSLRSTDEEDEDELIL

Target ORF information:

RefSeq Version	XM_011241199.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 1 (Clcn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241199.1

ORF Insert Sequence:

ATGAGACCGT GCCTGGGCAG CTTGATCTCC TGGTGCCAGC CTGTGCAGTG GGCGTGGGAT 
GCTACTTTGC AGCCCCTGAT GGAGCAGCCA TACTATTACA CTGACATCCT GACAGTGGGC 
TGTGCCGTGG GGGTCGGTTG CTGTTTTGGA ACACCACTTG GAGGAGTGCT ATTTAGCATC 
GAGGTCACCT CTACCTACTT TGCTGTTCGG AATTACTGGC GAGGATTCTT TGCAGCCACA 
TTCAGTGCCT TTGTGTTCCG TGTCCTGGCC GTTTGGAACA AGGATGCTGT CACCATCACT 
GCTCTGTTCA GAACGAATTT CCGAATGGAT TTCCCCTTTG ACCTGAAGGA ACTCCCAGCT 
TTTGCTGTCA TTGGGATTTG CTGCGGGTTC TTGGGAGCTG TTTTCGTTTA TCTGCATCGC 
CAAGTCATGC TCGGTGTCCG AAAGCACAAG TGTCTCAGCC AATTTCTTGC TAAGCACCGC 
CTGCTATATC CTGGAATTGT TACCTTTGTC ATCGCCTCGC TCACATTTCC ACCAGGAATG 
GGTCAATTCA TGGCTGGAGA GCTGATGCCC CGTGAAGCTA TCAGTACCCT CTTTGACAAC 
AACACATGGG TAAAGCACAT AGGTGACCCC CAAAGCTTGG GCCAGTCAGC TGTGTGGCTT 
CACCCCCAGG TCAACGTGAT CATCATCATC CTTCTCTTCT TCGTTATGAA GTTTTGGATG 
TCCATTGTAG CCACCACTAT GCCCATACCC TGTGGAGGCT TCATGCCTGT TTTTGTGCTA 
GGAGCTGCAT TTGGAAGGCT GGTAGGAGAG ATCATGGCCA TGCTATTCCC TGAGGGTATC 
TTATTTGATG ATATCATCTA TAAGATCTTA CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA 
GCTTTGACAG GGGCTGTCTC CCACACAGTC TCCACAGCCG TCATTTGCTT CGAATTAACC 
GGTCAGATTG CTCACATCCT GCCCATGATG GTGGCTGTTA TCTTGGCCAA CATGGTGGCT 
CAGAGTCTGC AGCCCTCCCT CTATGACAGC ATCATCCAGG TCAAGAAGCT TCCCTATCTG 
CCAGACCTTG GTTGGAACCA GCTCAGCAAA TTTACAATTT TTGTTGAGGA CATCATGGTA 
CGTGATGTGA AGTTCGTTTC AGCTTCTTGT ACATATGGGG AACTGAGAAA CCTACTCCAG 
GCCACCACAG TCAAGACTTT ACCATTGGTT GACTCCAAAG ATTCAATGAT CCTGCTGGGC 
TCTGTGGAAC GCTCAGAACT GCAGTCCCTC CTGCAGCGCC ACCTGTGTGC AGAGCGAAGG 
TTGAAGGCTG CCCAGGACAT GGCTCGAAAG TTATCAGAGC TGCCTTATAA TGGCAAGGCT 
CAGCTGGCTG GGGACTGGCA TCCTGGTGGT CGACCTGAGT CCTTTGCCTT CGTAGATGAA 
GATGAAGATG AGGACCTCTC CAGGAAGATG GAGCTTCCAC TGACTCCAGC TCCTCCCCCT 
CCTTCCCCTC CTCCTCCACC TTCCCAGTTT CCTATTGCTC CATCAAACCC TGAAGAACCT 
AATGGGCCAC TGCCCAGCCA TAAACAGCCA CCTGAAGCCT CAGACTCTGC AGATCAAAGA 
TCATCCACCT TCCAACGCCT GCTGCACTGC TTGCTAGGAA AAGCTCACTC CAAAAAGAAG 
AAAATAACAC AGGATTCAAC AGATTTAGTG GATAACATGT CACCTGAAGA GATTGAGGCC 
TGGGAGCGGG AGCAGCTGAG CCAGCCTGTG TGCTTTGATT GCTGCTGCAT CGACCAGTCT 
CCCTTCCAGC TGGTGGAGCA GACAACCCTG CACAAGACTC ATACACTCTT CTCACTTCTT 
GGCCTCCATC TTGCCTATGT GACCAGCATG GGGAAGCTCA GAGGTGTCTT GGCACTAGAA 
GAGCTACAGA AAGCTATTGA GGGCCACACC AAATCTGGGG TGCAGCTTCG CCCTCCACTT 
GCCAGCTTCC GGAATACAAC TTCAATCCGG AAGACTCCTG GGGGGCCACC CCCTCCTGCA 
GAGGGCTGGA ATGTACCTGA GGATGGAGAT GGGGCTCCTG GAAGAGAAGT CATGGTTCCT 
ACCATGCCAG AGACTCCTGT CCCACCACCA TCTCCAGAGG CCCCTTCCTG CCTGGCCCCA 
GCCAGAGCGG AGGGTGAGCT GGAGGAACTG GAGATGGTGG GGAGCCTAGA GCCTGAGGAG 
GAGCTGGCTG ACATCTTGCA TGGCCCCAGT CTGCGGTCCA CTGATGAGGA AGATGAGGAC 
GAGCTGATCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dmpk gene deletion or antisense knockdown does not compromise cardiac or skeletal muscle function in mice.
Human molecular genetics25(19)4328-4338(2016 10)
Carrell ST,Carrell EM,Auerbach D,Pandey SK,Bennett CF,Dirksen RT,Thornton CA

Variant mapping and mutation discovery in inbred mice using next-generation sequencing.
BMC genomics16913(2015 Nov)
Gallego-Llamas J,Timms AE,Geister KA,Lindsay A,Beier DR

Cooperation meets competition in microRNA-mediated DMPK transcript regulation.
Nucleic acids research43(19)9500-18(2015 Oct)
Koscianska E,Witkos TM,Kozlowska E,Wojciechowska M,Krzyzosiak WJ

Muscleblind-Like 1 and Muscleblind-Like 3 Depletion Synergistically Enhances Myotonia by Altering Clc-1 RNA Translation.
EBioMedicine2(9)1034-47(2015 Sep)
Choi J,Personius KE,DiFranco M,Dansithong W,Yu C,Srivastava S,Dixon DM,Bhatt DB,Comai L,Vergara JL,Reddy S

NKX2-5, a modifier of skeletal muscle pathology due to RNA toxicity.
Human molecular genetics24(1)251-64(2015 Jan)
Gladman JT,Yadava RS,Mandal M,Yu Q,Kim YK,Mahadevan MS

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Clcn1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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