CKMT1 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	CKMT1
Entrez Gene ID	12716
Full Name	creatine kinase, mitochondrial 1, ubiquitous
Synonyms	Mt-CK,ScCKmit,UbCKmit,mi-CK
General protein information	Preferred Names creatine kinase, mitochondrial 1, ubiquitous Names creatine kinase U-type, mitochondrial U-MtCK acidic-type mitochondrial creatine kinase mia-CK ubiquitous mitochondrial creatine kinase
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 2 Map Location: 2 E5\|2 60.37 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009897.2	NP_034027.1	creatine kinase U-type, mitochondrial precursor
XM_006498656.2	XP_006498719.1	creatine kinase U-type, mitochondrial isoform X1
NM_009897.3	NP_034027.1	creatine kinase U-type, mitochondrial precursor
NM_001355069.1	NP_001341998.1	creatine kinase U-type, mitochondrial precursor
XM_006498656.3	XP_006498719.1	creatine kinase U-type, mitochondrial isoform X1
XM_006498655.1	XP_006498718.1	creatine kinase U-type, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CKMT1B	NP_066270.1
Pan troglodytes (chimpanzee)	CKMT1B	XP_003314688.1
Pan troglodytes (chimpanzee)	LOC100608872	XP_003314691.1
Homo sapiens (human)	CKMT1A	NP_001015001.1
	LOC712886	XP_002808494.1
Bos taurus (cattle)	CKMT1B	NP_776700.1
Xenopus tropicalis (tropical clawed frog)	ckmt1b	NP_989369.1
Rattus norvegicus (Norway rat)	Ckmt1b	NP_001012756.1
Gallus gallus (chicken)	CKMT1A	NP_989513.1
Danio rerio (zebrafish)	ckmt1	NP_942096.1
Canis lupus familiaris (dog)	CKMT1B	XP_535451.1
Mus musculus (house mouse)	Ckmt1	NP_034027.1

Gene Ontology
Bibliography

Related articles in PubMed

Cell-Type-Specific Spatiotemporal Expression of Creatine Biosynthetic Enzyme S-adenosylmethionine:guanidinoacetate N-methyltransferase in Developing Mouse Brain.
Tachikawa M, Watanabe M, Fukaya M, Sakai K, Terasaki T, Hosoya KI
Neurochemical research(2017 Dec)

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Rutledge EA, Benazet JD, McMahon AP
Development (Cambridge, England)144(17)3177-3188(2017 09)

In the absence of phosphate shuttling, exercise reveals the in vivo importance of creatine-independent mitochondrial ADP transport.
Miotto PM, Holloway GP
The Biochemical journal473(18)2831-43(2016 09)

Differential expression of adenine nucleotide converting enzymes in mitochondrial intermembrane space: a potential role of adenylate kinase isozyme 2 in neutrophil differentiation.
Tanimura A, Horiguchi T, Miyoshi K, Hagita H, Noma T
PloS one9(2)e89916(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mitochondrial creatine kinase expression in hepatocellular carcinoma may be caused by hepatocarcinogenesis per se but not by loss of mitochondrial integrity, of which ASB9 could be a negative regulator
Title: High ubiquitous mitochondrial creatine kinase expression in hepatocellular carcinoma denotes a poor prognosis with highly malignant potential.

the knock-down of the mitochondrial creatine kinase-1 (CKMT1) by RNA interference causes the dissipation of the mitochondrial membrane potential
Title: Reconstitution of CKMT1 expression fails to rescue cells from mitochondrial membrane potential dissipation: implications for controlling RNAi experiments.

Hypothalamic plasticity of neuropeptide Y is lacking in brain-type creatine kinase double knockout mice with defective thermoregulation
Title: Hypothalamic plasticity of neuropeptide Y is lacking in brain-type creatine kinase double knockout mice with defective thermoregulation.

Mt-CK(-/-) on a C57BL/6 background do not develop LV hypertrophy or dysfunction even up to 1 year, and this may be explained by a compensatory increase in MM-CK activity and mitochondrial volume
Title: Cardiac phenotype of mitochondrial creatine kinase knockout mice is modified on a pure C57BL/6 genetic background.

Data suggest that amyloid precursor protein may regulate cellular energy levels and mitochondrial function via a direct interaction and stabilization of ubiquitous mitochondrial creatine kinase.
Title: Stabilization of ubiquitous mitochondrial creatine kinase preprotein by APP family proteins.

The following CKMT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CKMT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16757	NM_001355069.1 Latest version!	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu16757	NM_009897.3 Latest version!	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu16757	XM_006498656.3 Latest version!	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu16757	XM_006498655.1 Latest version!	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
View Old Accession Versions >>
OMu16757	NM_009897.2	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu16757	XM_006498656.2	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CKMT1 ( NM_009897.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_034027.1 Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16757
Clone ID Related Accession (Same CDS sequence)	NM_009897.2 , XM_006498656.2 , NM_009897.3 , NM_001355069.1 , XM_006498656.3 , XM_006498655.1
Accession Version	NM_009897.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Mus musculus(house mouse)
Product	creatine kinase U-type, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL845466.4. On Nov 17, 2006 this sequence version replaced NM_009897.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z13968.1, AK005076.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034027.1
CDS	210..1466
Translation	MAGPFSRLLSARPGLRLLALAGAGSLTAGILLRPESVGAAAAERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFAELFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVEKLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDNRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPPPLVHSKH

Target ORF information:

RefSeq Version	NM_009897.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009897.2

ORF Insert Sequence:

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKMT1 ( NM_009897.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_034027.1 Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16757
Clone ID Related Accession (Same CDS sequence)	NM_009897.2 , XM_006498656.2 , NM_009897.3 , NM_001355069.1 , XM_006498656.3 , XM_006498655.1
Accession Version	NM_009897.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Mus musculus(house mouse)
Product	creatine kinase U-type, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845466.4. On Mar 26, 2018 this sequence version replaced NM_009897.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z13968.1, AK131953.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034027.1
CDS	241..1497
Translation	MAGPFSRLLSARPGLRLLALAGAGSLTAGILLRPESVGAAAAERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFAELFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVEKLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDNRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPPPLVHSKH

Target ORF information:

RefSeq Version	NM_009897.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009897.3

ORF Insert Sequence:

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKMT1 ( NM_001355069.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001341998.1 Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16757
Clone ID Related Accession (Same CDS sequence)	NM_009897.2 , XM_006498656.2 , NM_009897.3 , NM_001355069.1 , XM_006498656.3 , XM_006498655.1
Accession Version	NM_001355069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	creatine kinase U-type, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845466.4. On Sep 6, 2017 this sequence version replaced XM_006498655.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC025976.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341998.1
CDS	139..1395
Translation	MAGPFSRLLSARPGLRLLALAGAGSLTAGILLRPESVGAAAAERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFAELFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVEKLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDNRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPPPLVHSKH

Target ORF information:

RefSeq Version	NM_001355069.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355069.1

ORF Insert Sequence:

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKMT1 ( XM_006498656.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006498719.1 Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu16757
Clone ID Related Accession (Same CDS sequence)	NM_009897.2 , XM_006498656.2 , NM_009897.3 , NM_001355069.1 , XM_006498656.3 , XM_006498655.1
Accession Version	XM_006498656.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	creatine kinase U-type, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498719.1
CDS	74..1330
Translation	MAGPFSRLLSARPGLRLLALAGAGSLTAGILLRPESVGAAAAERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFAELFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVEKLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDNRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPPPLVHSKH

Target ORF information:

RefSeq Version	XM_006498656.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498656.2

ORF Insert Sequence:

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKMT1 ( XM_006498656.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006498719.1 Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16757
Clone ID Related Accession (Same CDS sequence)	NM_009897.2 , XM_006498656.2 , NM_009897.3 , NM_001355069.1 , XM_006498656.3 , XM_006498655.1
Accession Version	XM_006498656.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	creatine kinase U-type, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498656.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498719.1
CDS	231..1487
Translation	MAGPFSRLLSARPGLRLLALAGAGSLTAGILLRPESVGAAAAERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFAELFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVEKLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDNRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPPPLVHSKH

Target ORF information:

RefSeq Version	XM_006498656.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498656.3

ORF Insert Sequence:

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKMT1 ( XM_006498655.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006498718.1 Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16757
Clone ID Related Accession (Same CDS sequence)	NM_009897.2 , XM_006498656.2 , NM_009897.3 , NM_001355069.1 , XM_006498656.3 , XM_006498655.1
Accession Version	XM_006498655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	creatine kinase U-type, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498718.1
CDS	164..1420
Translation	MAGPFSRLLSARPGLRLLALAGAGSLTAGILLRPESVGAAAAERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFAELFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVEKLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDNRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPPPLVHSKH

Target ORF information:

RefSeq Version	XM_006498655.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498655.1

ORF Insert Sequence:

ATGGCTGGTC CCTTCTCCCG TCTGCTGTCT GCCCGCCCTG GACTCAGGCT CCTGGCTTTG 
GCTGGAGCTG GGTCTCTCAC CGCCGGGATT CTGCTCCGCC CGGAATCTGT AGGAGCTGCC 
GCTGCTGAAC GGAGGAGACT GTATCCCCCG AGCGCTGAGT ACCCAGACCT CCGAAAGCAC 
AACAACTGCA TGGCCAGTCA CCTGACCCCA GCAGTCTATG CACGGCTCTG CGACAAGACC 
ACACCCACTG GTTGGACACT AGATCAGTGC ATCCAGACTG GAGTGGACAA CCCTGGCCAC 
CCCTTCATCA AGACTGTGGG CATGGTGGCT GGAGATGAGG AGACCTATGA GGTATTTGCT 
GAACTGTTTG ACCCTGTGAT CCAAGAGCGG CATAATGGAT ATGACCCCAG AACAATGAAG 
CACACCACTG ACCTTGATGC CAGTAAAATC CGTTCTGGCT ACTTTGATGA GAGGTATGTA 
TTGTCTTCAA GAGTCAGAAC TGGCCGAAGT ATCAGGGGAC TCAGTCTCCC TCCAGCCTGC 
ACTCGGGCAG AGCGAAGAGA GGTAGAACGT GTTGTGGTGG ATGCTCTGAG TGGCCTGAAG 
GGTGACCTGG CTGGACGGTA CTATAGGCTC AGTGAGATGA CGGAGGCCGA ACAGCAGCAG 
CTTATTGATG ACCATTTTCT GTTTGATAAA CCTGTGTCCC CATTGCTGAC TGCAGCAGGA 
ATGGCTCGAG ACTGGCCTGA TGCTCGAGGG ATCTGGCACA ACAATGAGAA GAGTTTCTTG 
ATCTGGGTGA ATGAGGAGGA CCACACACGG GTCATCTCTA TGGAGAAAGG CGGCAACATG 
AAGAGAGTGT TTGAAAGATT CTGCCGGGGC CTCAAAGAGG TGGAGAAGCT GATCCAGGAA 
CGAGGCTGGG AGTTCATGTG GAATGAGCGT TTAGGCTACA TCTTGACCTG TCCATCTAAC 
CTGGGCACTG GACTTCGGGC AGGAGTCCAC ATCAAACTGC CACTGCTGAG CAAAGATAAC 
CGCTTCCCAA AGATCCTGGA GAACCTAAGA CTGCAAAAGC GTGGAACTGG AGGAGTGGAC 
ACAGCTGCCA CAGGCAGCGT CTTTGACATC TCTAATTTGG ATCGACTTGG CAAGTCAGAG 
GTGGAGCTGG TGCAGCTCGT CATCGATGGG GTGAACTATT TGATTGACTG TGAACGGCGT 
CTGGAGAGAG GACAGGATAT TCGAATCCCT CCACCTCTTG TCCACAGCAA ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cell-Type-Specific Spatiotemporal Expression of Creatine Biosynthetic Enzyme S-adenosylmethionine:guanidinoacetate N-methyltransferase in Developing Mouse Brain.
Neurochemical research(2017 Dec)
Tachikawa M,Watanabe M,Fukaya M,Sakai K,Terasaki T,Hosoya KI

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Development (Cambridge, England)144(17)3177-3188(2017 09)
Rutledge EA,Benazet JD,McMahon AP

In the absence of phosphate shuttling, exercise reveals the in vivo importance of creatine-independent mitochondrial ADP transport.
The Biochemical journal473(18)2831-43(2016 09)
Miotto PM,Holloway GP

Differential expression of adenine nucleotide converting enzymes in mitochondrial intermembrane space: a potential role of adenylate kinase isozyme 2 in neutrophil differentiation.
PloS one9(2)e89916(2014)
Tanimura A,Horiguchi T,Miyoshi K,Hagita H,Noma T

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CKMT1 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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