Cftr cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cftr
Entrez Gene ID	12638
Full Name	cystic fibrosis transmembrane conductance regulator
Synonyms	AW495489,Abcc7
General protein information	Preferred Names cystic fibrosis transmembrane conductance regulator Names cystic fibrosis transmembrane conductance regulator ATP-binding cassette sub-family C member 7 ATP-binding cassette transporter sub-family C member 7 ATP-binding cassette, subfamily c, member 7 cAMP-dependent chloride channel channel conductance-controlling ATPase cystic fibrosis transmembrane conductance regulator homolog cystic fibrosis transmembrane conductance regulator homolog; ATP-binding cassette, subfamily c, member 7
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 A2\|6 8.1 cM
Summary	The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This gene encodes the cystic fibrosis transmembrane regulator and a chloride channel that controls the regulation of other transport pathways. Mutations in this gene have been associated with autosomal recessive disorders such as cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternative splicing of exons 4, 5, and 11 have been observed, but full-length transcripts have not yet been fully described. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006504979.3	XP_006505042.1	cystic fibrosis transmembrane conductance regulator isoform X2
XM_006504978.1	XP_006505041.1	cystic fibrosis transmembrane conductance regulator isoform X1
NM_021050.2	NP_066388.1	cystic fibrosis transmembrane conductance regulator
XM_006504977.1	XP_006505040.1	cystic fibrosis transmembrane conductance regulator isoform X1

Pathways from BioSystems

Homologies

	CFTR	NP_001028110.1
Pan troglodytes (chimpanzee)	CFTR	NP_001073386.1
Rattus norvegicus (Norway rat)	Cftr	NP_113694.1
Canis lupus familiaris (dog)	CFTR	NP_001007144.1
Mus musculus (house mouse)	Cftr	NP_066388.1
Xenopus tropicalis (tropical clawed frog)	cftr	XP_002935002.2
Homo sapiens (human)	CFTR	NP_000483.3
Bos taurus (cattle)	CFTR	NP_776443.1
Gallus gallus (chicken)	CFTR	NP_001099136.1
Danio rerio (zebrafish)	cftr	NP_001038348.1

Gene Ontology

The following Cftr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cftr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22220	NM_021050.2 Latest version!	Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu22220	XM_006504977.1 Latest version!	Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu22220	XM_006504978.1 Latest version!	Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu36508	XM_006504979.3 Latest version!	Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cftr ( NM_021050.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_066388.1 Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), mRNA.

Price & Availability↑

CloneID	OMu22220
Clone ID Related Accession (Same CDS sequence)	XM_006504978.1 , NM_021050.2 , XM_006504977.1
Accession Version	NM_021050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	cystic fibrosis transmembrane conductance regulator
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC161826.5, M60493.1 and AC158663.2. On Oct 10, 2006 this sequence version replaced NM_021050.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M60493.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAGT CGCCTTTGGA GAAAGCCAGC TTTATCTCCA AACTCTTCTT CAGCTGGACC 
ACACCAATTT TGAGGAAAGG GTACAGACAC CACTTGGAGT TGTCAGACAT ATACCAAGCC 
CCTTCTGCTG ATTCAGCTGA CCACTTGTCT GAAAAACTAG AAAGAGAATG GGACAGAGAA 
CAAGCTTCAA AAAAGAATCC CCAGCTTATC CACGCCCTTC GGCGATGCTT TTTCTGGAGA 
TTCCTCTTCT ATGGAATTTT GCTATACCTA GGGGAAGTCA CCAAGGCTGT CCAGCCTGTC 
TTGCTAGGAA GAATCATAGC ATCCTATGAT CCAGAAAACA AGGTGGAACG TTCCATTGCC 
ATTTACCTTG GCATAGGCTT ATGCCTTCTC TTCATTGTCA GGACACTGCT TCTTCACCCA 
GCTATTTTTG GCCTTCATCG CATTGGAATG CAGATGAGAA CAGCTATGTT TAGCTTGATT 
TATAAGAAGA CTTTAAAGTT GTCAAGCCGC GTTCTTGATA AAATAAGTAT TGGACAACTT 
GTTAGTCTTC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCCTT GGCACATTTT 
ATATGGATTG CTCCTTTACA AGTGACTCTT CTGATGGGGC TTCTCTGGGA CTTGTTACAG 
TTCTCAGCCT TCTGTGGCCT TGGTTTACTG ATAATCCTGG TTATTTTTCA AGCTATCCTA 
GGGAAGATGA TGGTGAAGTA CAGAGATCAG AGAGCTGCAA AGATCAATGA AAGACTCGTG 
ATCACATCAG AAATTATTGA TAATATCTAT TCTGTTAAGG CATATTGTTG GGAATCAGCG 
ATGGAGAAAA TGATTGAAAA CTTGAGAGAG GTGGAGCTGA AAATGACCCG GAAGGCGGCC 
TATATGAGGT TCTTCACTAG CTCTGCCTTC TTCTTTTCAG GGTTCTTTGT AGTCTTTCTA 
TCTGTGCTTC CCTACACAGT CATCAACGGA ATCGTCCTAC GAAAAATATT CACAACCATT 
TCATTCTGCA TTGTCCTACG TATGTCAGTC ACACGGCAGT TCCCCACTGC CGTACAGATA 
TGGTATGATT CTTTTGGAAT GATAAGAAAA ATACAGGATT TCCTGCAGAA ACAAGAGTAT 
AAAGTACTGG AGTATAACTT AATGACCACA GGCATAATCA TGGAAAATGT AACAGCATTT 
TGGGAGGAGG GATTTGGGGA ATTACTGGAG AAAGTACAAC AAAGCAATGG TGACAGAAAA 
CATTCCAGTG ATGAGAACAA TGTCAGTTTC AGTCATCTCT GCCTTGTGGG AAATCCTGTG 
CTGAAAAACA TCAATTTGAA TATAGAGAAA GGAGAGATGT TGGCTATTAC TGGATCTACT 
GGATCAGGAA AGACATCACT CCTGATGTTG ATTTTGGGAG AACTGGAAGC TTCAGAGGGA 
ATTATTAAGC ACAGTGGAAG AGTTTCATTC TGCTCTCAAT TTTCTTGGAT TATGCCGGGT 
ACTATCAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AGTACAGATA TAAGAGTGTT 
GTCAAAGCTT GCCAACTACA GCAGGACATC ACCAAGTTTG CAGAACAAGA CAACACAGTT 
CTTGGAGAAG GTGGAGTCAC ACTGAGTGGA GGTCAGCGTG CAAGGATTTC TTTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTGTACCTA TTAGATTCCC CTTTTGGATA TCTAGATGTT 
TTTACTGAAG AACAAGTATT TGAAAGCTGT GTTTGTAAAT TGATGGCCAA CAAAACTAGG 
ATTTTGGTTA CATCTAAAAT GGAACACTTA AGGAAAGCTG ACAAAATACT AATTTTGCAT 
CAGGGCAGTA GCTATTTTTA TGGGACATTT TCTGAGCTAC AAAGTCTACG TCCAGACTTC 
AGTTCGAAAC TCATGGGGTA TGATACTTTT GACCAGTTTA CTGAGGAAAG AAGAAGTTCA 
ATTCTAACTG AGACCTTACG CAGGTTCTCA GTAGACGATT CCTCTGCCCC GTGGAGCAAA 
CCCAAACAGT CGTTTAGACA GACTGGAGAG GTGGGAGAAA AAAGGAAGAA CTCTATTCTA 
AATTCATTCA GCTCTGTAAG GAAAATTTCC ATTGTGCAAA AGACTCCATT ATGTATCGAT 
GGAGAGTCTG ATGATCTCCA AGAAAAGAGA CTGTCCCTAG TTCCGGATTC TGAACAGGGG 
GAGGCTGCTC TGCCGCGCAG CAACATGATC GCCACCGGCC CCACATTTCC AGGCAGAAGA 
AGACAGTCTG TTTTGGATCT GATGACGTTC ACACCCAACT CAGGCTCCAG CAATCTTCAG 
AGGACCAGAA CTTCTATTCG AAAAATCTCC TTAGTCCCTC AGATAAGCTT AAATGAAGTG 
GATGTATATT CAAGGAGATT ATCGCAAGAT AGCACACTGA ACATCACTGA AGAAATTAAC 
GAAGAAGATT TAAAGGAGTG TTTTCTTGAT GATGTGATCA AGATACCCCC GGTGACAACA 
TGGAACACAT ACCTACGATA TTTTACTCTC CATAAAGGCT TACTGCTAGT GCTGATTTGG 
TGCGTACTGG TTTTTCTGGT TGAGGTGGCT GCTTCTTTAT TTGTGTTATG GTTGCTTAAA 
AACAACCCTG TTAACAGTGG AAACAATGGT ACTAAAATTT CCAATAGCTC CTATGTTGTG 
ATCATCACCA GTACCAGTTT CTATTATATT TTTTACATTT ACGTGGGAGT GGCTGACACT 
TTGCTTGCCC TGAGCCTCTT CAGAGGTTTG CCGCTGGTGC ATACGTTAAT CACAGCATCA 
AAAATTTTGC ACAGGAAAAT GTTACACTCC ATTCTTCACG CCCCTATGTC GACCATCAGC 
AAGCTGAAAG CAGGTGGGAT TCTTAACAGA TTCTCCAAAG ATATAGCAAT TTTGGATGAC 
TTTCTGCCTC TTACCATTTT TGACTTCATT CAGTTGGTGT TCATTGTGAT TGGAGCTATA 
ATAGTCGTCT CGGCATTACA ACCCTACATC TTCCTAGCAA CGGTGCCAGG GCTAGTAGTC 
TTTATTTTAC TGAGGGCCTA CTTCCTTCAT ACAGCACAGC AGCTCAAACA ACTGGAATCT 
GAAGGCAGGA GTCCAATTTT CACCCACCTT GTGACAAGCT TAAAAGGACT CTGGACACTT 
CGAGCCTTCC GACGCCAGAC TTACTTTGAA ACTCTGTTCC ACAAAGCTCT GAATTTGCAC 
ACTGCCAACT GGTTTATGTA TCTGGCAACC TTGCGCTGGT TCCAAATGAG AATAGACATG 
ATATTTGTCC TCTTCTTCAT TGTTGTTACC TTCATCTCCA TTTTAACAAC AGGTGAAGGA 
GAAGGAACAG CTGGTATTAT TCTAACTTTA GCTATGAATA TCATGAGTAC TTTGCAGTGG 
GCTGTGAACT CAAGCATTGA TACAGATAGC TTGATGCGAT CTGTGAGCAG AGTGTTTAAG 
TTTATTGATA TACAAACAGA AGAAAGTATG TACACACAGA TAATTAAAGA ACTACCTAGA 
GAAGGATCAT CTGACGTTTT AGTCATTAAG AATGAGCATG TGAAGAAAAG TGATATCTGG 
CCCTCTGGAG GCGAAATGGT TGTCAAAGAC CTTACTGTGA AATACATGGA TGATGGAAAT 
GCCGTATTAG AGAACATTTC TTTTTCAATA AGTCCTGGAC AGAGGGTGGG GCTCTTAGGA 
AGAACTGGAT CAGGAAAAAG TACTTTGCTT TCAGCATTTT TACGAATGTT GAACATTAAA 
GGTGATATAG AGATTGATGG TGTCTCATGG AATTCAGTGA CCTTACAAGA ATGGAGGAAA 
GCTTTCGGAG TGATAACACA GAAAGTATTT ATCTTTTCTG GAACATTCAG ACAAAACCTG 
GATCCCAATG GAAAATGGAA AGATGAAGAA ATATGGAAAG TTGCAGATGA GGTTGGACTC 
AAGTCTGTAA TAGAGCAGTT TCCTGGACAG CTCAACTTTA CCCTTGTGGA TGGGGGTTAT 
GTGCTAAGCC ATGGCCATAA GCAATTAATG TGCTTGGCCC GATCAGTTCT CAGTAAGGCC 
AAGATCATAC TGCTTGATGA GCCCAGTGCC CATCTAGACC CCATAACATA CCAAGTCATT 
CGACGAGTTC TAAAACAAGC CTTCGCTGGT TGCACAGTCA TCCTCTGTGA ACACAGGATA 
GAAGCGATGT TGGATTGCCA GCGATTTTTG GTCATAGAAG AGAGCAATGT CTGGCAGTAC 
GACTCCCTTC AGGCACTTCT GAGTGAGAAG AGTATCTTCC AGCAGGCCAT TAGCTCCTCG 
GAAAAGATGA GGTTCTTCCA GGGCCGCCAC TCCAGCAAGC ACAAGCCTCG GACGCAAATT 
ACTGCTCTGA AAGAGGAGAC AGAAGAAGAA GTTCAAGAAA CCCGTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066388.1
CDS	138..4568
Translation	MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSADSADHLSEKLEREWDREQASKKNPQLIHALRRCFFWRFLFYGILLYLGEVTKAVQPVLLGRIIASYDPENKVERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHRIGMQMRTAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFIWIAPLQVTLLMGLLWDLLQFSAFCGLGLLIILVIFQAILGKMMVKYRDQRAAKINERLVITSEIIDNIYSVKAYCWESAMEKMIENLREVELKMTRKAAYMRFFTSSAFFFSGFFVVFLSVLPYTVINGIVLRKIFTTISFCIVLRMSVTRQFPTAVQIWYDSFGMIRKIQDFLQKQEYKVLEYNLMTTGIIMENVTAFWEEGFGELLEKVQQSNGDRKHSSDENNVSFSHLCLVGNPVLKNINLNIEKGEMLAITGSTGSGKTSLLMLILGELEASEGIIKHSGRVSFCSQFSWIMPGTIKENIIFGVSYDEYRYKSVVKACQLQQDITKFAEQDNTVLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVFTEEQVFESCVCKLMANKTRILVTSKMEHLRKADKILILHQGSSYFYGTFSELQSLRPDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDSSAPWSKPKQSFRQTGEVGEKRKNSILNSFSSVRKISIVQKTPLCIDGESDDLQEKRLSLVPDSEQGEAALPRSNMIATGPTFPGRRRQSVLDLMTFTPNSGSSNLQRTRTSIRKISLVPQISLNEVDVYSRRLSQDSTLNITEEINEEDLKECFLDDVIKIPPVTTWNTYLRYFTLHKGLLLVLIWCVLVFLVEVAASLFVLWLLKNNPVNSGNNGTKISNSSYVVIITSTSFYYIFYIYVGVADTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMSTISKLKAGGILNRFSKDIAILDDFLPLTIFDFIQLVFIVIGAIIVVSALQPYIFLATVPGLVVFILLRAYFLHTAQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYLATLRWFQMRIDMIFVLFFIVVTFISILTTGEGEGTAGIILTLAMNIMSTLQWAVNSSIDTDSLMRSVSRVFKFIDIQTEESMYTQIIKELPREGSSDVLVIKNEHVKKSDIWPSGGEMVVKDLTVKYMDDGNAVLENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGDIEIDGVSWNSVTLQEWRKAFGVITQKVFIFSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLNFTLVDGGYVLSHGHKQLMCLARSVLSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCEHRIEAMLDCQRFLVIEESNVWQYDSLQALLSEKSIFQQAISSSEKMRFFQGRHSSKHKPRTQITALKEETEEEVQETRL

Target ORF information:

RefSeq Version	NM_021050.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021050.2

ORF Insert Sequence:

ATGCAGAAGT CGCCTTTGGA GAAAGCCAGC TTTATCTCCA AACTCTTCTT CAGCTGGACC 
ACACCAATTT TGAGGAAAGG GTACAGACAC CACTTGGAGT TGTCAGACAT ATACCAAGCC 
CCTTCTGCTG ATTCAGCTGA CCACTTGTCT GAAAAACTAG AAAGAGAATG GGACAGAGAA 
CAAGCTTCAA AAAAGAATCC CCAGCTTATC CACGCCCTTC GGCGATGCTT TTTCTGGAGA 
TTCCTCTTCT ATGGAATTTT GCTATACCTA GGGGAAGTCA CCAAGGCTGT CCAGCCTGTC 
TTGCTAGGAA GAATCATAGC ATCCTATGAT CCAGAAAACA AGGTGGAACG TTCCATTGCC 
ATTTACCTTG GCATAGGCTT ATGCCTTCTC TTCATTGTCA GGACACTGCT TCTTCACCCA 
GCTATTTTTG GCCTTCATCG CATTGGAATG CAGATGAGAA CAGCTATGTT TAGCTTGATT 
TATAAGAAGA CTTTAAAGTT GTCAAGCCGC GTTCTTGATA AAATAAGTAT TGGACAACTT 
GTTAGTCTTC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCCTT GGCACATTTT 
ATATGGATTG CTCCTTTACA AGTGACTCTT CTGATGGGGC TTCTCTGGGA CTTGTTACAG 
TTCTCAGCCT TCTGTGGCCT TGGTTTACTG ATAATCCTGG TTATTTTTCA AGCTATCCTA 
GGGAAGATGA TGGTGAAGTA CAGAGATCAG AGAGCTGCAA AGATCAATGA AAGACTCGTG 
ATCACATCAG AAATTATTGA TAATATCTAT TCTGTTAAGG CATATTGTTG GGAATCAGCG 
ATGGAGAAAA TGATTGAAAA CTTGAGAGAG GTGGAGCTGA AAATGACCCG GAAGGCGGCC 
TATATGAGGT TCTTCACTAG CTCTGCCTTC TTCTTTTCAG GGTTCTTTGT AGTCTTTCTA 
TCTGTGCTTC CCTACACAGT CATCAACGGA ATCGTCCTAC GAAAAATATT CACAACCATT 
TCATTCTGCA TTGTCCTACG TATGTCAGTC ACACGGCAGT TCCCCACTGC CGTACAGATA 
TGGTATGATT CTTTTGGAAT GATAAGAAAA ATACAGGATT TCCTGCAGAA ACAAGAGTAT 
AAAGTACTGG AGTATAACTT AATGACCACA GGCATAATCA TGGAAAATGT AACAGCATTT 
TGGGAGGAGG GATTTGGGGA ATTACTGGAG AAAGTACAAC AAAGCAATGG TGACAGAAAA 
CATTCCAGTG ATGAGAACAA TGTCAGTTTC AGTCATCTCT GCCTTGTGGG AAATCCTGTG 
CTGAAAAACA TCAATTTGAA TATAGAGAAA GGAGAGATGT TGGCTATTAC TGGATCTACT 
GGATCAGGAA AGACATCACT CCTGATGTTG ATTTTGGGAG AACTGGAAGC TTCAGAGGGA 
ATTATTAAGC ACAGTGGAAG AGTTTCATTC TGCTCTCAAT TTTCTTGGAT TATGCCGGGT 
ACTATCAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AGTACAGATA TAAGAGTGTT 
GTCAAAGCTT GCCAACTACA GCAGGACATC ACCAAGTTTG CAGAACAAGA CAACACAGTT 
CTTGGAGAAG GTGGAGTCAC ACTGAGTGGA GGTCAGCGTG CAAGGATTTC TTTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTGTACCTA TTAGATTCCC CTTTTGGATA TCTAGATGTT 
TTTACTGAAG AACAAGTATT TGAAAGCTGT GTTTGTAAAT TGATGGCCAA CAAAACTAGG 
ATTTTGGTTA CATCTAAAAT GGAACACTTA AGGAAAGCTG ACAAAATACT AATTTTGCAT 
CAGGGCAGTA GCTATTTTTA TGGGACATTT TCTGAGCTAC AAAGTCTACG TCCAGACTTC 
AGTTCGAAAC TCATGGGGTA TGATACTTTT GACCAGTTTA CTGAGGAAAG AAGAAGTTCA 
ATTCTAACTG AGACCTTACG CAGGTTCTCA GTAGACGATT CCTCTGCCCC GTGGAGCAAA 
CCCAAACAGT CGTTTAGACA GACTGGAGAG GTGGGAGAAA AAAGGAAGAA CTCTATTCTA 
AATTCATTCA GCTCTGTAAG GAAAATTTCC ATTGTGCAAA AGACTCCATT ATGTATCGAT 
GGAGAGTCTG ATGATCTCCA AGAAAAGAGA CTGTCCCTAG TTCCGGATTC TGAACAGGGG 
GAGGCTGCTC TGCCGCGCAG CAACATGATC GCCACCGGCC CCACATTTCC AGGCAGAAGA 
AGACAGTCTG TTTTGGATCT GATGACGTTC ACACCCAACT CAGGCTCCAG CAATCTTCAG 
AGGACCAGAA CTTCTATTCG AAAAATCTCC TTAGTCCCTC AGATAAGCTT AAATGAAGTG 
GATGTATATT CAAGGAGATT ATCGCAAGAT AGCACACTGA ACATCACTGA AGAAATTAAC 
GAAGAAGATT TAAAGGAGTG TTTTCTTGAT GATGTGATCA AGATACCCCC GGTGACAACA 
TGGAACACAT ACCTACGATA TTTTACTCTC CATAAAGGCT TACTGCTAGT GCTGATTTGG 
TGCGTACTGG TTTTTCTGGT TGAGGTGGCT GCTTCTTTAT TTGTGTTATG GTTGCTTAAA 
AACAACCCTG TTAACAGTGG AAACAATGGT ACTAAAATTT CCAATAGCTC CTATGTTGTG 
ATCATCACCA GTACCAGTTT CTATTATATT TTTTACATTT ACGTGGGAGT GGCTGACACT 
TTGCTTGCCC TGAGCCTCTT CAGAGGTTTG CCGCTGGTGC ATACGTTAAT CACAGCATCA 
AAAATTTTGC ACAGGAAAAT GTTACACTCC ATTCTTCACG CCCCTATGTC GACCATCAGC 
AAGCTGAAAG CAGGTGGGAT TCTTAACAGA TTCTCCAAAG ATATAGCAAT TTTGGATGAC 
TTTCTGCCTC TTACCATTTT TGACTTCATT CAGTTGGTGT TCATTGTGAT TGGAGCTATA 
ATAGTCGTCT CGGCATTACA ACCCTACATC TTCCTAGCAA CGGTGCCAGG GCTAGTAGTC 
TTTATTTTAC TGAGGGCCTA CTTCCTTCAT ACAGCACAGC AGCTCAAACA ACTGGAATCT 
GAAGGCAGGA GTCCAATTTT CACCCACCTT GTGACAAGCT TAAAAGGACT CTGGACACTT 
CGAGCCTTCC GACGCCAGAC TTACTTTGAA ACTCTGTTCC ACAAAGCTCT GAATTTGCAC 
ACTGCCAACT GGTTTATGTA TCTGGCAACC TTGCGCTGGT TCCAAATGAG AATAGACATG 
ATATTTGTCC TCTTCTTCAT TGTTGTTACC TTCATCTCCA TTTTAACAAC AGGTGAAGGA 
GAAGGAACAG CTGGTATTAT TCTAACTTTA GCTATGAATA TCATGAGTAC TTTGCAGTGG 
GCTGTGAACT CAAGCATTGA TACAGATAGC TTGATGCGAT CTGTGAGCAG AGTGTTTAAG 
TTTATTGATA TACAAACAGA AGAAAGTATG TACACACAGA TAATTAAAGA ACTACCTAGA 
GAAGGATCAT CTGACGTTTT AGTCATTAAG AATGAGCATG TGAAGAAAAG TGATATCTGG 
CCCTCTGGAG GCGAAATGGT TGTCAAAGAC CTTACTGTGA AATACATGGA TGATGGAAAT 
GCCGTATTAG AGAACATTTC TTTTTCAATA AGTCCTGGAC AGAGGGTGGG GCTCTTAGGA 
AGAACTGGAT CAGGAAAAAG TACTTTGCTT TCAGCATTTT TACGAATGTT GAACATTAAA 
GGTGATATAG AGATTGATGG TGTCTCATGG AATTCAGTGA CCTTACAAGA ATGGAGGAAA 
GCTTTCGGAG TGATAACACA GAAAGTATTT ATCTTTTCTG GAACATTCAG ACAAAACCTG 
GATCCCAATG GAAAATGGAA AGATGAAGAA ATATGGAAAG TTGCAGATGA GGTTGGACTC 
AAGTCTGTAA TAGAGCAGTT TCCTGGACAG CTCAACTTTA CCCTTGTGGA TGGGGGTTAT 
GTGCTAAGCC ATGGCCATAA GCAATTAATG TGCTTGGCCC GATCAGTTCT CAGTAAGGCC 
AAGATCATAC TGCTTGATGA GCCCAGTGCC CATCTAGACC CCATAACATA CCAAGTCATT 
CGACGAGTTC TAAAACAAGC CTTCGCTGGT TGCACAGTCA TCCTCTGTGA ACACAGGATA 
GAAGCGATGT TGGATTGCCA GCGATTTTTG GTCATAGAAG AGAGCAATGT CTGGCAGTAC 
GACTCCCTTC AGGCACTTCT GAGTGAGAAG AGTATCTTCC AGCAGGCCAT TAGCTCCTCG 
GAAAAGATGA GGTTCTTCCA GGGCCGCCAC TCCAGCAAGC ACAAGCCTCG GACGCAAATT 
ACTGCTCTGA AAGAGGAGAC AGAAGAAGAA GTTCAAGAAA CCCGTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cftr ( XM_006504978.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505041.1 Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu22220
Clone ID Related Accession (Same CDS sequence)	XM_006504978.1 , NM_021050.2 , XM_006504977.1
Accession Version	XM_006504978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cystic fibrosis transmembrane conductance regulator isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGT CGCCTTTGGA GAAAGCCAGC TTTATCTCCA AACTCTTCTT CAGCTGGACC 
ACACCAATTT TGAGGAAAGG GTACAGACAC CACTTGGAGT TGTCAGACAT ATACCAAGCC 
CCTTCTGCTG ATTCAGCTGA CCACTTGTCT GAAAAACTAG AAAGAGAATG GGACAGAGAA 
CAAGCTTCAA AAAAGAATCC CCAGCTTATC CACGCCCTTC GGCGATGCTT TTTCTGGAGA 
TTCCTCTTCT ATGGAATTTT GCTATACCTA GGGGAAGTCA CCAAGGCTGT CCAGCCTGTC 
TTGCTAGGAA GAATCATAGC ATCCTATGAT CCAGAAAACA AGGTGGAACG TTCCATTGCC 
ATTTACCTTG GCATAGGCTT ATGCCTTCTC TTCATTGTCA GGACACTGCT TCTTCACCCA 
GCTATTTTTG GCCTTCATCG CATTGGAATG CAGATGAGAA CAGCTATGTT TAGCTTGATT 
TATAAGAAGA CTTTAAAGTT GTCAAGCCGC GTTCTTGATA AAATAAGTAT TGGACAACTT 
GTTAGTCTTC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCCTT GGCACATTTT 
ATATGGATTG CTCCTTTACA AGTGACTCTT CTGATGGGGC TTCTCTGGGA CTTGTTACAG 
TTCTCAGCCT TCTGTGGCCT TGGTTTACTG ATAATCCTGG TTATTTTTCA AGCTATCCTA 
GGGAAGATGA TGGTGAAGTA CAGAGATCAG AGAGCTGCAA AGATCAATGA AAGACTCGTG 
ATCACATCAG AAATTATTGA TAATATCTAT TCTGTTAAGG CATATTGTTG GGAATCAGCG 
ATGGAGAAAA TGATTGAAAA CTTGAGAGAG GTGGAGCTGA AAATGACCCG GAAGGCGGCC 
TATATGAGGT TCTTCACTAG CTCTGCCTTC TTCTTTTCAG GGTTCTTTGT AGTCTTTCTA 
TCTGTGCTTC CCTACACAGT CATCAACGGA ATCGTCCTAC GAAAAATATT CACAACCATT 
TCATTCTGCA TTGTCCTACG TATGTCAGTC ACACGGCAGT TCCCCACTGC CGTACAGATA 
TGGTATGATT CTTTTGGAAT GATAAGAAAA ATACAGGATT TCCTGCAGAA ACAAGAGTAT 
AAAGTACTGG AGTATAACTT AATGACCACA GGCATAATCA TGGAAAATGT AACAGCATTT 
TGGGAGGAGG GATTTGGGGA ATTACTGGAG AAAGTACAAC AAAGCAATGG TGACAGAAAA 
CATTCCAGTG ATGAGAACAA TGTCAGTTTC AGTCATCTCT GCCTTGTGGG AAATCCTGTG 
CTGAAAAACA TCAATTTGAA TATAGAGAAA GGAGAGATGT TGGCTATTAC TGGATCTACT 
GGATCAGGAA AGACATCACT CCTGATGTTG ATTTTGGGAG AACTGGAAGC TTCAGAGGGA 
ATTATTAAGC ACAGTGGAAG AGTTTCATTC TGCTCTCAAT TTTCTTGGAT TATGCCGGGT 
ACTATCAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AGTACAGATA TAAGAGTGTT 
GTCAAAGCTT GCCAACTACA GCAGGACATC ACCAAGTTTG CAGAACAAGA CAACACAGTT 
CTTGGAGAAG GTGGAGTCAC ACTGAGTGGA GGTCAGCGTG CAAGGATTTC TTTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTGTACCTA TTAGATTCCC CTTTTGGATA TCTAGATGTT 
TTTACTGAAG AACAAGTATT TGAAAGCTGT GTTTGTAAAT TGATGGCCAA CAAAACTAGG 
ATTTTGGTTA CATCTAAAAT GGAACACTTA AGGAAAGCTG ACAAAATACT AATTTTGCAT 
CAGGGCAGTA GCTATTTTTA TGGGACATTT TCTGAGCTAC AAAGTCTACG TCCAGACTTC 
AGTTCGAAAC TCATGGGGTA TGATACTTTT GACCAGTTTA CTGAGGAAAG AAGAAGTTCA 
ATTCTAACTG AGACCTTACG CAGGTTCTCA GTAGACGATT CCTCTGCCCC GTGGAGCAAA 
CCCAAACAGT CGTTTAGACA GACTGGAGAG GTGGGAGAAA AAAGGAAGAA CTCTATTCTA 
AATTCATTCA GCTCTGTAAG GAAAATTTCC ATTGTGCAAA AGACTCCATT ATGTATCGAT 
GGAGAGTCTG ATGATCTCCA AGAAAAGAGA CTGTCCCTAG TTCCGGATTC TGAACAGGGG 
GAGGCTGCTC TGCCGCGCAG CAACATGATC GCCACCGGCC CCACATTTCC AGGCAGAAGA 
AGACAGTCTG TTTTGGATCT GATGACGTTC ACACCCAACT CAGGCTCCAG CAATCTTCAG 
AGGACCAGAA CTTCTATTCG AAAAATCTCC TTAGTCCCTC AGATAAGCTT AAATGAAGTG 
GATGTATATT CAAGGAGATT ATCGCAAGAT AGCACACTGA ACATCACTGA AGAAATTAAC 
GAAGAAGATT TAAAGGAGTG TTTTCTTGAT GATGTGATCA AGATACCCCC GGTGACAACA 
TGGAACACAT ACCTACGATA TTTTACTCTC CATAAAGGCT TACTGCTAGT GCTGATTTGG 
TGCGTACTGG TTTTTCTGGT TGAGGTGGCT GCTTCTTTAT TTGTGTTATG GTTGCTTAAA 
AACAACCCTG TTAACAGTGG AAACAATGGT ACTAAAATTT CCAATAGCTC CTATGTTGTG 
ATCATCACCA GTACCAGTTT CTATTATATT TTTTACATTT ACGTGGGAGT GGCTGACACT 
TTGCTTGCCC TGAGCCTCTT CAGAGGTTTG CCGCTGGTGC ATACGTTAAT CACAGCATCA 
AAAATTTTGC ACAGGAAAAT GTTACACTCC ATTCTTCACG CCCCTATGTC GACCATCAGC 
AAGCTGAAAG CAGGTGGGAT TCTTAACAGA TTCTCCAAAG ATATAGCAAT TTTGGATGAC 
TTTCTGCCTC TTACCATTTT TGACTTCATT CAGTTGGTGT TCATTGTGAT TGGAGCTATA 
ATAGTCGTCT CGGCATTACA ACCCTACATC TTCCTAGCAA CGGTGCCAGG GCTAGTAGTC 
TTTATTTTAC TGAGGGCCTA CTTCCTTCAT ACAGCACAGC AGCTCAAACA ACTGGAATCT 
GAAGGCAGGA GTCCAATTTT CACCCACCTT GTGACAAGCT TAAAAGGACT CTGGACACTT 
CGAGCCTTCC GACGCCAGAC TTACTTTGAA ACTCTGTTCC ACAAAGCTCT GAATTTGCAC 
ACTGCCAACT GGTTTATGTA TCTGGCAACC TTGCGCTGGT TCCAAATGAG AATAGACATG 
ATATTTGTCC TCTTCTTCAT TGTTGTTACC TTCATCTCCA TTTTAACAAC AGGTGAAGGA 
GAAGGAACAG CTGGTATTAT TCTAACTTTA GCTATGAATA TCATGAGTAC TTTGCAGTGG 
GCTGTGAACT CAAGCATTGA TACAGATAGC TTGATGCGAT CTGTGAGCAG AGTGTTTAAG 
TTTATTGATA TACAAACAGA AGAAAGTATG TACACACAGA TAATTAAAGA ACTACCTAGA 
GAAGGATCAT CTGACGTTTT AGTCATTAAG AATGAGCATG TGAAGAAAAG TGATATCTGG 
CCCTCTGGAG GCGAAATGGT TGTCAAAGAC CTTACTGTGA AATACATGGA TGATGGAAAT 
GCCGTATTAG AGAACATTTC TTTTTCAATA AGTCCTGGAC AGAGGGTGGG GCTCTTAGGA 
AGAACTGGAT CAGGAAAAAG TACTTTGCTT TCAGCATTTT TACGAATGTT GAACATTAAA 
GGTGATATAG AGATTGATGG TGTCTCATGG AATTCAGTGA CCTTACAAGA ATGGAGGAAA 
GCTTTCGGAG TGATAACACA GAAAGTATTT ATCTTTTCTG GAACATTCAG ACAAAACCTG 
GATCCCAATG GAAAATGGAA AGATGAAGAA ATATGGAAAG TTGCAGATGA GGTTGGACTC 
AAGTCTGTAA TAGAGCAGTT TCCTGGACAG CTCAACTTTA CCCTTGTGGA TGGGGGTTAT 
GTGCTAAGCC ATGGCCATAA GCAATTAATG TGCTTGGCCC GATCAGTTCT CAGTAAGGCC 
AAGATCATAC TGCTTGATGA GCCCAGTGCC CATCTAGACC CCATAACATA CCAAGTCATT 
CGACGAGTTC TAAAACAAGC CTTCGCTGGT TGCACAGTCA TCCTCTGTGA ACACAGGATA 
GAAGCGATGT TGGATTGCCA GCGATTTTTG GTCATAGAAG AGAGCAATGT CTGGCAGTAC 
GACTCCCTTC AGGCACTTCT GAGTGAGAAG AGTATCTTCC AGCAGGCCAT TAGCTCCTCG 
GAAAAGATGA GGTTCTTCCA GGGCCGCCAC TCCAGCAAGC ACAAGCCTCG GACGCAAATT 
ACTGCTCTGA AAGAGGAGAC AGAAGAAGAA GTTCAAGAAA CCCGTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505041.1
CDS	225..4655
Translation	MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSADSADHLSEKLEREWDREQASKKNPQLIHALRRCFFWRFLFYGILLYLGEVTKAVQPVLLGRIIASYDPENKVERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHRIGMQMRTAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFIWIAPLQVTLLMGLLWDLLQFSAFCGLGLLIILVIFQAILGKMMVKYRDQRAAKINERLVITSEIIDNIYSVKAYCWESAMEKMIENLREVELKMTRKAAYMRFFTSSAFFFSGFFVVFLSVLPYTVINGIVLRKIFTTISFCIVLRMSVTRQFPTAVQIWYDSFGMIRKIQDFLQKQEYKVLEYNLMTTGIIMENVTAFWEEGFGELLEKVQQSNGDRKHSSDENNVSFSHLCLVGNPVLKNINLNIEKGEMLAITGSTGSGKTSLLMLILGELEASEGIIKHSGRVSFCSQFSWIMPGTIKENIIFGVSYDEYRYKSVVKACQLQQDITKFAEQDNTVLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVFTEEQVFESCVCKLMANKTRILVTSKMEHLRKADKILILHQGSSYFYGTFSELQSLRPDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDSSAPWSKPKQSFRQTGEVGEKRKNSILNSFSSVRKISIVQKTPLCIDGESDDLQEKRLSLVPDSEQGEAALPRSNMIATGPTFPGRRRQSVLDLMTFTPNSGSSNLQRTRTSIRKISLVPQISLNEVDVYSRRLSQDSTLNITEEINEEDLKECFLDDVIKIPPVTTWNTYLRYFTLHKGLLLVLIWCVLVFLVEVAASLFVLWLLKNNPVNSGNNGTKISNSSYVVIITSTSFYYIFYIYVGVADTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMSTISKLKAGGILNRFSKDIAILDDFLPLTIFDFIQLVFIVIGAIIVVSALQPYIFLATVPGLVVFILLRAYFLHTAQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYLATLRWFQMRIDMIFVLFFIVVTFISILTTGEGEGTAGIILTLAMNIMSTLQWAVNSSIDTDSLMRSVSRVFKFIDIQTEESMYTQIIKELPREGSSDVLVIKNEHVKKSDIWPSGGEMVVKDLTVKYMDDGNAVLENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGDIEIDGVSWNSVTLQEWRKAFGVITQKVFIFSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLNFTLVDGGYVLSHGHKQLMCLARSVLSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCEHRIEAMLDCQRFLVIEESNVWQYDSLQALLSEKSIFQQAISSSEKMRFFQGRHSSKHKPRTQITALKEETEEEVQETRL

Target ORF information:

RefSeq Version	XM_006504978.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504978.1

ORF Insert Sequence:

ATGCAGAAGT CGCCTTTGGA GAAAGCCAGC TTTATCTCCA AACTCTTCTT CAGCTGGACC 
ACACCAATTT TGAGGAAAGG GTACAGACAC CACTTGGAGT TGTCAGACAT ATACCAAGCC 
CCTTCTGCTG ATTCAGCTGA CCACTTGTCT GAAAAACTAG AAAGAGAATG GGACAGAGAA 
CAAGCTTCAA AAAAGAATCC CCAGCTTATC CACGCCCTTC GGCGATGCTT TTTCTGGAGA 
TTCCTCTTCT ATGGAATTTT GCTATACCTA GGGGAAGTCA CCAAGGCTGT CCAGCCTGTC 
TTGCTAGGAA GAATCATAGC ATCCTATGAT CCAGAAAACA AGGTGGAACG TTCCATTGCC 
ATTTACCTTG GCATAGGCTT ATGCCTTCTC TTCATTGTCA GGACACTGCT TCTTCACCCA 
GCTATTTTTG GCCTTCATCG CATTGGAATG CAGATGAGAA CAGCTATGTT TAGCTTGATT 
TATAAGAAGA CTTTAAAGTT GTCAAGCCGC GTTCTTGATA AAATAAGTAT TGGACAACTT 
GTTAGTCTTC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCCTT GGCACATTTT 
ATATGGATTG CTCCTTTACA AGTGACTCTT CTGATGGGGC TTCTCTGGGA CTTGTTACAG 
TTCTCAGCCT TCTGTGGCCT TGGTTTACTG ATAATCCTGG TTATTTTTCA AGCTATCCTA 
GGGAAGATGA TGGTGAAGTA CAGAGATCAG AGAGCTGCAA AGATCAATGA AAGACTCGTG 
ATCACATCAG AAATTATTGA TAATATCTAT TCTGTTAAGG CATATTGTTG GGAATCAGCG 
ATGGAGAAAA TGATTGAAAA CTTGAGAGAG GTGGAGCTGA AAATGACCCG GAAGGCGGCC 
TATATGAGGT TCTTCACTAG CTCTGCCTTC TTCTTTTCAG GGTTCTTTGT AGTCTTTCTA 
TCTGTGCTTC CCTACACAGT CATCAACGGA ATCGTCCTAC GAAAAATATT CACAACCATT 
TCATTCTGCA TTGTCCTACG TATGTCAGTC ACACGGCAGT TCCCCACTGC CGTACAGATA 
TGGTATGATT CTTTTGGAAT GATAAGAAAA ATACAGGATT TCCTGCAGAA ACAAGAGTAT 
AAAGTACTGG AGTATAACTT AATGACCACA GGCATAATCA TGGAAAATGT AACAGCATTT 
TGGGAGGAGG GATTTGGGGA ATTACTGGAG AAAGTACAAC AAAGCAATGG TGACAGAAAA 
CATTCCAGTG ATGAGAACAA TGTCAGTTTC AGTCATCTCT GCCTTGTGGG AAATCCTGTG 
CTGAAAAACA TCAATTTGAA TATAGAGAAA GGAGAGATGT TGGCTATTAC TGGATCTACT 
GGATCAGGAA AGACATCACT CCTGATGTTG ATTTTGGGAG AACTGGAAGC TTCAGAGGGA 
ATTATTAAGC ACAGTGGAAG AGTTTCATTC TGCTCTCAAT TTTCTTGGAT TATGCCGGGT 
ACTATCAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AGTACAGATA TAAGAGTGTT 
GTCAAAGCTT GCCAACTACA GCAGGACATC ACCAAGTTTG CAGAACAAGA CAACACAGTT 
CTTGGAGAAG GTGGAGTCAC ACTGAGTGGA GGTCAGCGTG CAAGGATTTC TTTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTGTACCTA TTAGATTCCC CTTTTGGATA TCTAGATGTT 
TTTACTGAAG AACAAGTATT TGAAAGCTGT GTTTGTAAAT TGATGGCCAA CAAAACTAGG 
ATTTTGGTTA CATCTAAAAT GGAACACTTA AGGAAAGCTG ACAAAATACT AATTTTGCAT 
CAGGGCAGTA GCTATTTTTA TGGGACATTT TCTGAGCTAC AAAGTCTACG TCCAGACTTC 
AGTTCGAAAC TCATGGGGTA TGATACTTTT GACCAGTTTA CTGAGGAAAG AAGAAGTTCA 
ATTCTAACTG AGACCTTACG CAGGTTCTCA GTAGACGATT CCTCTGCCCC GTGGAGCAAA 
CCCAAACAGT CGTTTAGACA GACTGGAGAG GTGGGAGAAA AAAGGAAGAA CTCTATTCTA 
AATTCATTCA GCTCTGTAAG GAAAATTTCC ATTGTGCAAA AGACTCCATT ATGTATCGAT 
GGAGAGTCTG ATGATCTCCA AGAAAAGAGA CTGTCCCTAG TTCCGGATTC TGAACAGGGG 
GAGGCTGCTC TGCCGCGCAG CAACATGATC GCCACCGGCC CCACATTTCC AGGCAGAAGA 
AGACAGTCTG TTTTGGATCT GATGACGTTC ACACCCAACT CAGGCTCCAG CAATCTTCAG 
AGGACCAGAA CTTCTATTCG AAAAATCTCC TTAGTCCCTC AGATAAGCTT AAATGAAGTG 
GATGTATATT CAAGGAGATT ATCGCAAGAT AGCACACTGA ACATCACTGA AGAAATTAAC 
GAAGAAGATT TAAAGGAGTG TTTTCTTGAT GATGTGATCA AGATACCCCC GGTGACAACA 
TGGAACACAT ACCTACGATA TTTTACTCTC CATAAAGGCT TACTGCTAGT GCTGATTTGG 
TGCGTACTGG TTTTTCTGGT TGAGGTGGCT GCTTCTTTAT TTGTGTTATG GTTGCTTAAA 
AACAACCCTG TTAACAGTGG AAACAATGGT ACTAAAATTT CCAATAGCTC CTATGTTGTG 
ATCATCACCA GTACCAGTTT CTATTATATT TTTTACATTT ACGTGGGAGT GGCTGACACT 
TTGCTTGCCC TGAGCCTCTT CAGAGGTTTG CCGCTGGTGC ATACGTTAAT CACAGCATCA 
AAAATTTTGC ACAGGAAAAT GTTACACTCC ATTCTTCACG CCCCTATGTC GACCATCAGC 
AAGCTGAAAG CAGGTGGGAT TCTTAACAGA TTCTCCAAAG ATATAGCAAT TTTGGATGAC 
TTTCTGCCTC TTACCATTTT TGACTTCATT CAGTTGGTGT TCATTGTGAT TGGAGCTATA 
ATAGTCGTCT CGGCATTACA ACCCTACATC TTCCTAGCAA CGGTGCCAGG GCTAGTAGTC 
TTTATTTTAC TGAGGGCCTA CTTCCTTCAT ACAGCACAGC AGCTCAAACA ACTGGAATCT 
GAAGGCAGGA GTCCAATTTT CACCCACCTT GTGACAAGCT TAAAAGGACT CTGGACACTT 
CGAGCCTTCC GACGCCAGAC TTACTTTGAA ACTCTGTTCC ACAAAGCTCT GAATTTGCAC 
ACTGCCAACT GGTTTATGTA TCTGGCAACC TTGCGCTGGT TCCAAATGAG AATAGACATG 
ATATTTGTCC TCTTCTTCAT TGTTGTTACC TTCATCTCCA TTTTAACAAC AGGTGAAGGA 
GAAGGAACAG CTGGTATTAT TCTAACTTTA GCTATGAATA TCATGAGTAC TTTGCAGTGG 
GCTGTGAACT CAAGCATTGA TACAGATAGC TTGATGCGAT CTGTGAGCAG AGTGTTTAAG 
TTTATTGATA TACAAACAGA AGAAAGTATG TACACACAGA TAATTAAAGA ACTACCTAGA 
GAAGGATCAT CTGACGTTTT AGTCATTAAG AATGAGCATG TGAAGAAAAG TGATATCTGG 
CCCTCTGGAG GCGAAATGGT TGTCAAAGAC CTTACTGTGA AATACATGGA TGATGGAAAT 
GCCGTATTAG AGAACATTTC TTTTTCAATA AGTCCTGGAC AGAGGGTGGG GCTCTTAGGA 
AGAACTGGAT CAGGAAAAAG TACTTTGCTT TCAGCATTTT TACGAATGTT GAACATTAAA 
GGTGATATAG AGATTGATGG TGTCTCATGG AATTCAGTGA CCTTACAAGA ATGGAGGAAA 
GCTTTCGGAG TGATAACACA GAAAGTATTT ATCTTTTCTG GAACATTCAG ACAAAACCTG 
GATCCCAATG GAAAATGGAA AGATGAAGAA ATATGGAAAG TTGCAGATGA GGTTGGACTC 
AAGTCTGTAA TAGAGCAGTT TCCTGGACAG CTCAACTTTA CCCTTGTGGA TGGGGGTTAT 
GTGCTAAGCC ATGGCCATAA GCAATTAATG TGCTTGGCCC GATCAGTTCT CAGTAAGGCC 
AAGATCATAC TGCTTGATGA GCCCAGTGCC CATCTAGACC CCATAACATA CCAAGTCATT 
CGACGAGTTC TAAAACAAGC CTTCGCTGGT TGCACAGTCA TCCTCTGTGA ACACAGGATA 
GAAGCGATGT TGGATTGCCA GCGATTTTTG GTCATAGAAG AGAGCAATGT CTGGCAGTAC 
GACTCCCTTC AGGCACTTCT GAGTGAGAAG AGTATCTTCC AGCAGGCCAT TAGCTCCTCG 
GAAAAGATGA GGTTCTTCCA GGGCCGCCAC TCCAGCAAGC ACAAGCCTCG GACGCAAATT 
ACTGCTCTGA AAGAGGAGAC AGAAGAAGAA GTTCAAGAAA CCCGTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cftr ( XM_006504977.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505040.1 Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X1, mRNA.

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CloneID	OMu22220
Clone ID Related Accession (Same CDS sequence)	XM_006504978.1 , NM_021050.2 , XM_006504977.1
Accession Version	XM_006504977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cystic fibrosis transmembrane conductance regulator isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGT CGCCTTTGGA GAAAGCCAGC TTTATCTCCA AACTCTTCTT CAGCTGGACC 
ACACCAATTT TGAGGAAAGG GTACAGACAC CACTTGGAGT TGTCAGACAT ATACCAAGCC 
CCTTCTGCTG ATTCAGCTGA CCACTTGTCT GAAAAACTAG AAAGAGAATG GGACAGAGAA 
CAAGCTTCAA AAAAGAATCC CCAGCTTATC CACGCCCTTC GGCGATGCTT TTTCTGGAGA 
TTCCTCTTCT ATGGAATTTT GCTATACCTA GGGGAAGTCA CCAAGGCTGT CCAGCCTGTC 
TTGCTAGGAA GAATCATAGC ATCCTATGAT CCAGAAAACA AGGTGGAACG TTCCATTGCC 
ATTTACCTTG GCATAGGCTT ATGCCTTCTC TTCATTGTCA GGACACTGCT TCTTCACCCA 
GCTATTTTTG GCCTTCATCG CATTGGAATG CAGATGAGAA CAGCTATGTT TAGCTTGATT 
TATAAGAAGA CTTTAAAGTT GTCAAGCCGC GTTCTTGATA AAATAAGTAT TGGACAACTT 
GTTAGTCTTC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCCTT GGCACATTTT 
ATATGGATTG CTCCTTTACA AGTGACTCTT CTGATGGGGC TTCTCTGGGA CTTGTTACAG 
TTCTCAGCCT TCTGTGGCCT TGGTTTACTG ATAATCCTGG TTATTTTTCA AGCTATCCTA 
GGGAAGATGA TGGTGAAGTA CAGAGATCAG AGAGCTGCAA AGATCAATGA AAGACTCGTG 
ATCACATCAG AAATTATTGA TAATATCTAT TCTGTTAAGG CATATTGTTG GGAATCAGCG 
ATGGAGAAAA TGATTGAAAA CTTGAGAGAG GTGGAGCTGA AAATGACCCG GAAGGCGGCC 
TATATGAGGT TCTTCACTAG CTCTGCCTTC TTCTTTTCAG GGTTCTTTGT AGTCTTTCTA 
TCTGTGCTTC CCTACACAGT CATCAACGGA ATCGTCCTAC GAAAAATATT CACAACCATT 
TCATTCTGCA TTGTCCTACG TATGTCAGTC ACACGGCAGT TCCCCACTGC CGTACAGATA 
TGGTATGATT CTTTTGGAAT GATAAGAAAA ATACAGGATT TCCTGCAGAA ACAAGAGTAT 
AAAGTACTGG AGTATAACTT AATGACCACA GGCATAATCA TGGAAAATGT AACAGCATTT 
TGGGAGGAGG GATTTGGGGA ATTACTGGAG AAAGTACAAC AAAGCAATGG TGACAGAAAA 
CATTCCAGTG ATGAGAACAA TGTCAGTTTC AGTCATCTCT GCCTTGTGGG AAATCCTGTG 
CTGAAAAACA TCAATTTGAA TATAGAGAAA GGAGAGATGT TGGCTATTAC TGGATCTACT 
GGATCAGGAA AGACATCACT CCTGATGTTG ATTTTGGGAG AACTGGAAGC TTCAGAGGGA 
ATTATTAAGC ACAGTGGAAG AGTTTCATTC TGCTCTCAAT TTTCTTGGAT TATGCCGGGT 
ACTATCAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AGTACAGATA TAAGAGTGTT 
GTCAAAGCTT GCCAACTACA GCAGGACATC ACCAAGTTTG CAGAACAAGA CAACACAGTT 
CTTGGAGAAG GTGGAGTCAC ACTGAGTGGA GGTCAGCGTG CAAGGATTTC TTTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTGTACCTA TTAGATTCCC CTTTTGGATA TCTAGATGTT 
TTTACTGAAG AACAAGTATT TGAAAGCTGT GTTTGTAAAT TGATGGCCAA CAAAACTAGG 
ATTTTGGTTA CATCTAAAAT GGAACACTTA AGGAAAGCTG ACAAAATACT AATTTTGCAT 
CAGGGCAGTA GCTATTTTTA TGGGACATTT TCTGAGCTAC AAAGTCTACG TCCAGACTTC 
AGTTCGAAAC TCATGGGGTA TGATACTTTT GACCAGTTTA CTGAGGAAAG AAGAAGTTCA 
ATTCTAACTG AGACCTTACG CAGGTTCTCA GTAGACGATT CCTCTGCCCC GTGGAGCAAA 
CCCAAACAGT CGTTTAGACA GACTGGAGAG GTGGGAGAAA AAAGGAAGAA CTCTATTCTA 
AATTCATTCA GCTCTGTAAG GAAAATTTCC ATTGTGCAAA AGACTCCATT ATGTATCGAT 
GGAGAGTCTG ATGATCTCCA AGAAAAGAGA CTGTCCCTAG TTCCGGATTC TGAACAGGGG 
GAGGCTGCTC TGCCGCGCAG CAACATGATC GCCACCGGCC CCACATTTCC AGGCAGAAGA 
AGACAGTCTG TTTTGGATCT GATGACGTTC ACACCCAACT CAGGCTCCAG CAATCTTCAG 
AGGACCAGAA CTTCTATTCG AAAAATCTCC TTAGTCCCTC AGATAAGCTT AAATGAAGTG 
GATGTATATT CAAGGAGATT ATCGCAAGAT AGCACACTGA ACATCACTGA AGAAATTAAC 
GAAGAAGATT TAAAGGAGTG TTTTCTTGAT GATGTGATCA AGATACCCCC GGTGACAACA 
TGGAACACAT ACCTACGATA TTTTACTCTC CATAAAGGCT TACTGCTAGT GCTGATTTGG 
TGCGTACTGG TTTTTCTGGT TGAGGTGGCT GCTTCTTTAT TTGTGTTATG GTTGCTTAAA 
AACAACCCTG TTAACAGTGG AAACAATGGT ACTAAAATTT CCAATAGCTC CTATGTTGTG 
ATCATCACCA GTACCAGTTT CTATTATATT TTTTACATTT ACGTGGGAGT GGCTGACACT 
TTGCTTGCCC TGAGCCTCTT CAGAGGTTTG CCGCTGGTGC ATACGTTAAT CACAGCATCA 
AAAATTTTGC ACAGGAAAAT GTTACACTCC ATTCTTCACG CCCCTATGTC GACCATCAGC 
AAGCTGAAAG CAGGTGGGAT TCTTAACAGA TTCTCCAAAG ATATAGCAAT TTTGGATGAC 
TTTCTGCCTC TTACCATTTT TGACTTCATT CAGTTGGTGT TCATTGTGAT TGGAGCTATA 
ATAGTCGTCT CGGCATTACA ACCCTACATC TTCCTAGCAA CGGTGCCAGG GCTAGTAGTC 
TTTATTTTAC TGAGGGCCTA CTTCCTTCAT ACAGCACAGC AGCTCAAACA ACTGGAATCT 
GAAGGCAGGA GTCCAATTTT CACCCACCTT GTGACAAGCT TAAAAGGACT CTGGACACTT 
CGAGCCTTCC GACGCCAGAC TTACTTTGAA ACTCTGTTCC ACAAAGCTCT GAATTTGCAC 
ACTGCCAACT GGTTTATGTA TCTGGCAACC TTGCGCTGGT TCCAAATGAG AATAGACATG 
ATATTTGTCC TCTTCTTCAT TGTTGTTACC TTCATCTCCA TTTTAACAAC AGGTGAAGGA 
GAAGGAACAG CTGGTATTAT TCTAACTTTA GCTATGAATA TCATGAGTAC TTTGCAGTGG 
GCTGTGAACT CAAGCATTGA TACAGATAGC TTGATGCGAT CTGTGAGCAG AGTGTTTAAG 
TTTATTGATA TACAAACAGA AGAAAGTATG TACACACAGA TAATTAAAGA ACTACCTAGA 
GAAGGATCAT CTGACGTTTT AGTCATTAAG AATGAGCATG TGAAGAAAAG TGATATCTGG 
CCCTCTGGAG GCGAAATGGT TGTCAAAGAC CTTACTGTGA AATACATGGA TGATGGAAAT 
GCCGTATTAG AGAACATTTC TTTTTCAATA AGTCCTGGAC AGAGGGTGGG GCTCTTAGGA 
AGAACTGGAT CAGGAAAAAG TACTTTGCTT TCAGCATTTT TACGAATGTT GAACATTAAA 
GGTGATATAG AGATTGATGG TGTCTCATGG AATTCAGTGA CCTTACAAGA ATGGAGGAAA 
GCTTTCGGAG TGATAACACA GAAAGTATTT ATCTTTTCTG GAACATTCAG ACAAAACCTG 
GATCCCAATG GAAAATGGAA AGATGAAGAA ATATGGAAAG TTGCAGATGA GGTTGGACTC 
AAGTCTGTAA TAGAGCAGTT TCCTGGACAG CTCAACTTTA CCCTTGTGGA TGGGGGTTAT 
GTGCTAAGCC ATGGCCATAA GCAATTAATG TGCTTGGCCC GATCAGTTCT CAGTAAGGCC 
AAGATCATAC TGCTTGATGA GCCCAGTGCC CATCTAGACC CCATAACATA CCAAGTCATT 
CGACGAGTTC TAAAACAAGC CTTCGCTGGT TGCACAGTCA TCCTCTGTGA ACACAGGATA 
GAAGCGATGT TGGATTGCCA GCGATTTTTG GTCATAGAAG AGAGCAATGT CTGGCAGTAC 
GACTCCCTTC AGGCACTTCT GAGTGAGAAG AGTATCTTCC AGCAGGCCAT TAGCTCCTCG 
GAAAAGATGA GGTTCTTCCA GGGCCGCCAC TCCAGCAAGC ACAAGCCTCG GACGCAAATT 
ACTGCTCTGA AAGAGGAGAC AGAAGAAGAA GTTCAAGAAA CCCGTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505040.1
CDS	277..4707
Translation	MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSADSADHLSEKLEREWDREQASKKNPQLIHALRRCFFWRFLFYGILLYLGEVTKAVQPVLLGRIIASYDPENKVERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHRIGMQMRTAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFIWIAPLQVTLLMGLLWDLLQFSAFCGLGLLIILVIFQAILGKMMVKYRDQRAAKINERLVITSEIIDNIYSVKAYCWESAMEKMIENLREVELKMTRKAAYMRFFTSSAFFFSGFFVVFLSVLPYTVINGIVLRKIFTTISFCIVLRMSVTRQFPTAVQIWYDSFGMIRKIQDFLQKQEYKVLEYNLMTTGIIMENVTAFWEEGFGELLEKVQQSNGDRKHSSDENNVSFSHLCLVGNPVLKNINLNIEKGEMLAITGSTGSGKTSLLMLILGELEASEGIIKHSGRVSFCSQFSWIMPGTIKENIIFGVSYDEYRYKSVVKACQLQQDITKFAEQDNTVLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVFTEEQVFESCVCKLMANKTRILVTSKMEHLRKADKILILHQGSSYFYGTFSELQSLRPDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDSSAPWSKPKQSFRQTGEVGEKRKNSILNSFSSVRKISIVQKTPLCIDGESDDLQEKRLSLVPDSEQGEAALPRSNMIATGPTFPGRRRQSVLDLMTFTPNSGSSNLQRTRTSIRKISLVPQISLNEVDVYSRRLSQDSTLNITEEINEEDLKECFLDDVIKIPPVTTWNTYLRYFTLHKGLLLVLIWCVLVFLVEVAASLFVLWLLKNNPVNSGNNGTKISNSSYVVIITSTSFYYIFYIYVGVADTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMSTISKLKAGGILNRFSKDIAILDDFLPLTIFDFIQLVFIVIGAIIVVSALQPYIFLATVPGLVVFILLRAYFLHTAQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYLATLRWFQMRIDMIFVLFFIVVTFISILTTGEGEGTAGIILTLAMNIMSTLQWAVNSSIDTDSLMRSVSRVFKFIDIQTEESMYTQIIKELPREGSSDVLVIKNEHVKKSDIWPSGGEMVVKDLTVKYMDDGNAVLENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGDIEIDGVSWNSVTLQEWRKAFGVITQKVFIFSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLNFTLVDGGYVLSHGHKQLMCLARSVLSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCEHRIEAMLDCQRFLVIEESNVWQYDSLQALLSEKSIFQQAISSSEKMRFFQGRHSSKHKPRTQITALKEETEEEVQETRL

Target ORF information:

RefSeq Version	XM_006504977.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504977.1

ORF Insert Sequence:

ATGCAGAAGT CGCCTTTGGA GAAAGCCAGC TTTATCTCCA AACTCTTCTT CAGCTGGACC 
ACACCAATTT TGAGGAAAGG GTACAGACAC CACTTGGAGT TGTCAGACAT ATACCAAGCC 
CCTTCTGCTG ATTCAGCTGA CCACTTGTCT GAAAAACTAG AAAGAGAATG GGACAGAGAA 
CAAGCTTCAA AAAAGAATCC CCAGCTTATC CACGCCCTTC GGCGATGCTT TTTCTGGAGA 
TTCCTCTTCT ATGGAATTTT GCTATACCTA GGGGAAGTCA CCAAGGCTGT CCAGCCTGTC 
TTGCTAGGAA GAATCATAGC ATCCTATGAT CCAGAAAACA AGGTGGAACG TTCCATTGCC 
ATTTACCTTG GCATAGGCTT ATGCCTTCTC TTCATTGTCA GGACACTGCT TCTTCACCCA 
GCTATTTTTG GCCTTCATCG CATTGGAATG CAGATGAGAA CAGCTATGTT TAGCTTGATT 
TATAAGAAGA CTTTAAAGTT GTCAAGCCGC GTTCTTGATA AAATAAGTAT TGGACAACTT 
GTTAGTCTTC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCCTT GGCACATTTT 
ATATGGATTG CTCCTTTACA AGTGACTCTT CTGATGGGGC TTCTCTGGGA CTTGTTACAG 
TTCTCAGCCT TCTGTGGCCT TGGTTTACTG ATAATCCTGG TTATTTTTCA AGCTATCCTA 
GGGAAGATGA TGGTGAAGTA CAGAGATCAG AGAGCTGCAA AGATCAATGA AAGACTCGTG 
ATCACATCAG AAATTATTGA TAATATCTAT TCTGTTAAGG CATATTGTTG GGAATCAGCG 
ATGGAGAAAA TGATTGAAAA CTTGAGAGAG GTGGAGCTGA AAATGACCCG GAAGGCGGCC 
TATATGAGGT TCTTCACTAG CTCTGCCTTC TTCTTTTCAG GGTTCTTTGT AGTCTTTCTA 
TCTGTGCTTC CCTACACAGT CATCAACGGA ATCGTCCTAC GAAAAATATT CACAACCATT 
TCATTCTGCA TTGTCCTACG TATGTCAGTC ACACGGCAGT TCCCCACTGC CGTACAGATA 
TGGTATGATT CTTTTGGAAT GATAAGAAAA ATACAGGATT TCCTGCAGAA ACAAGAGTAT 
AAAGTACTGG AGTATAACTT AATGACCACA GGCATAATCA TGGAAAATGT AACAGCATTT 
TGGGAGGAGG GATTTGGGGA ATTACTGGAG AAAGTACAAC AAAGCAATGG TGACAGAAAA 
CATTCCAGTG ATGAGAACAA TGTCAGTTTC AGTCATCTCT GCCTTGTGGG AAATCCTGTG 
CTGAAAAACA TCAATTTGAA TATAGAGAAA GGAGAGATGT TGGCTATTAC TGGATCTACT 
GGATCAGGAA AGACATCACT CCTGATGTTG ATTTTGGGAG AACTGGAAGC TTCAGAGGGA 
ATTATTAAGC ACAGTGGAAG AGTTTCATTC TGCTCTCAAT TTTCTTGGAT TATGCCGGGT 
ACTATCAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AGTACAGATA TAAGAGTGTT 
GTCAAAGCTT GCCAACTACA GCAGGACATC ACCAAGTTTG CAGAACAAGA CAACACAGTT 
CTTGGAGAAG GTGGAGTCAC ACTGAGTGGA GGTCAGCGTG CAAGGATTTC TTTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTGTACCTA TTAGATTCCC CTTTTGGATA TCTAGATGTT 
TTTACTGAAG AACAAGTATT TGAAAGCTGT GTTTGTAAAT TGATGGCCAA CAAAACTAGG 
ATTTTGGTTA CATCTAAAAT GGAACACTTA AGGAAAGCTG ACAAAATACT AATTTTGCAT 
CAGGGCAGTA GCTATTTTTA TGGGACATTT TCTGAGCTAC AAAGTCTACG TCCAGACTTC 
AGTTCGAAAC TCATGGGGTA TGATACTTTT GACCAGTTTA CTGAGGAAAG AAGAAGTTCA 
ATTCTAACTG AGACCTTACG CAGGTTCTCA GTAGACGATT CCTCTGCCCC GTGGAGCAAA 
CCCAAACAGT CGTTTAGACA GACTGGAGAG GTGGGAGAAA AAAGGAAGAA CTCTATTCTA 
AATTCATTCA GCTCTGTAAG GAAAATTTCC ATTGTGCAAA AGACTCCATT ATGTATCGAT 
GGAGAGTCTG ATGATCTCCA AGAAAAGAGA CTGTCCCTAG TTCCGGATTC TGAACAGGGG 
GAGGCTGCTC TGCCGCGCAG CAACATGATC GCCACCGGCC CCACATTTCC AGGCAGAAGA 
AGACAGTCTG TTTTGGATCT GATGACGTTC ACACCCAACT CAGGCTCCAG CAATCTTCAG 
AGGACCAGAA CTTCTATTCG AAAAATCTCC TTAGTCCCTC AGATAAGCTT AAATGAAGTG 
GATGTATATT CAAGGAGATT ATCGCAAGAT AGCACACTGA ACATCACTGA AGAAATTAAC 
GAAGAAGATT TAAAGGAGTG TTTTCTTGAT GATGTGATCA AGATACCCCC GGTGACAACA 
TGGAACACAT ACCTACGATA TTTTACTCTC CATAAAGGCT TACTGCTAGT GCTGATTTGG 
TGCGTACTGG TTTTTCTGGT TGAGGTGGCT GCTTCTTTAT TTGTGTTATG GTTGCTTAAA 
AACAACCCTG TTAACAGTGG AAACAATGGT ACTAAAATTT CCAATAGCTC CTATGTTGTG 
ATCATCACCA GTACCAGTTT CTATTATATT TTTTACATTT ACGTGGGAGT GGCTGACACT 
TTGCTTGCCC TGAGCCTCTT CAGAGGTTTG CCGCTGGTGC ATACGTTAAT CACAGCATCA 
AAAATTTTGC ACAGGAAAAT GTTACACTCC ATTCTTCACG CCCCTATGTC GACCATCAGC 
AAGCTGAAAG CAGGTGGGAT TCTTAACAGA TTCTCCAAAG ATATAGCAAT TTTGGATGAC 
TTTCTGCCTC TTACCATTTT TGACTTCATT CAGTTGGTGT TCATTGTGAT TGGAGCTATA 
ATAGTCGTCT CGGCATTACA ACCCTACATC TTCCTAGCAA CGGTGCCAGG GCTAGTAGTC 
TTTATTTTAC TGAGGGCCTA CTTCCTTCAT ACAGCACAGC AGCTCAAACA ACTGGAATCT 
GAAGGCAGGA GTCCAATTTT CACCCACCTT GTGACAAGCT TAAAAGGACT CTGGACACTT 
CGAGCCTTCC GACGCCAGAC TTACTTTGAA ACTCTGTTCC ACAAAGCTCT GAATTTGCAC 
ACTGCCAACT GGTTTATGTA TCTGGCAACC TTGCGCTGGT TCCAAATGAG AATAGACATG 
ATATTTGTCC TCTTCTTCAT TGTTGTTACC TTCATCTCCA TTTTAACAAC AGGTGAAGGA 
GAAGGAACAG CTGGTATTAT TCTAACTTTA GCTATGAATA TCATGAGTAC TTTGCAGTGG 
GCTGTGAACT CAAGCATTGA TACAGATAGC TTGATGCGAT CTGTGAGCAG AGTGTTTAAG 
TTTATTGATA TACAAACAGA AGAAAGTATG TACACACAGA TAATTAAAGA ACTACCTAGA 
GAAGGATCAT CTGACGTTTT AGTCATTAAG AATGAGCATG TGAAGAAAAG TGATATCTGG 
CCCTCTGGAG GCGAAATGGT TGTCAAAGAC CTTACTGTGA AATACATGGA TGATGGAAAT 
GCCGTATTAG AGAACATTTC TTTTTCAATA AGTCCTGGAC AGAGGGTGGG GCTCTTAGGA 
AGAACTGGAT CAGGAAAAAG TACTTTGCTT TCAGCATTTT TACGAATGTT GAACATTAAA 
GGTGATATAG AGATTGATGG TGTCTCATGG AATTCAGTGA CCTTACAAGA ATGGAGGAAA 
GCTTTCGGAG TGATAACACA GAAAGTATTT ATCTTTTCTG GAACATTCAG ACAAAACCTG 
GATCCCAATG GAAAATGGAA AGATGAAGAA ATATGGAAAG TTGCAGATGA GGTTGGACTC 
AAGTCTGTAA TAGAGCAGTT TCCTGGACAG CTCAACTTTA CCCTTGTGGA TGGGGGTTAT 
GTGCTAAGCC ATGGCCATAA GCAATTAATG TGCTTGGCCC GATCAGTTCT CAGTAAGGCC 
AAGATCATAC TGCTTGATGA GCCCAGTGCC CATCTAGACC CCATAACATA CCAAGTCATT 
CGACGAGTTC TAAAACAAGC CTTCGCTGGT TGCACAGTCA TCCTCTGTGA ACACAGGATA 
GAAGCGATGT TGGATTGCCA GCGATTTTTG GTCATAGAAG AGAGCAATGT CTGGCAGTAC 
GACTCCCTTC AGGCACTTCT GAGTGAGAAG AGTATCTTCC AGCAGGCCAT TAGCTCCTCG 
GAAAAGATGA GGTTCTTCCA GGGCCGCCAC TCCAGCAAGC ACAAGCCTCG GACGCAAATT 
ACTGCTCTGA AAGAGGAGAC AGAAGAAGAA GTTCAAGAAA CCCGTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cftr ( XM_006504979.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505042.1 Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X4, mRNA.

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CloneID	OMu36508
Clone ID Related Accession (Same CDS sequence)	XM_006504979.3
Accession Version	XM_006504979.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cystic fibrosis transmembrane conductance regulator isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504979.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGT CGCCTTTGGA GAAAGCCAGC TTTATCTCCA AACTCTTCTT CAGCTGGACC 
ACACCAATTT TGAGGAAAGG GTACAGACAC CACTTGGAGT TGTCAGACAT ATACCAAGCC 
CCTTCTGCTG ATTCAGCTGA CCACTTGTCT GAAAAACTAG AAAGAGAATG GGACAGAGAA 
CAAGCTTCAA AAAAGAATCC CCAGCTTATC CACGCCCTTC GGCGATGCTT TTTCTGGAGA 
TTCCTCTTCT ATGGAATTTT GCTATACCTA GGGGAAGTCA CCAAGGCTGT CCAGCCTGTC 
TTGCTAGGAA GAATCATAGC ATCCTATGAT CCAGAAAACA AGGTGGAACG TTCCATTGCC 
ATTTACCTTG GCATAGGCTT ATGCCTTCTC TTCATTGTCA GGACACTGCT TCTTCACCCA 
GCTATTTTTG GCCTTCATCG CATTGGAATG CAGATGAGAA CAGCTATGTT TAGCTTGATT 
TATAAGAAGA CTTTAAAGTT GTCAAGCCGC GTTCTTGATA AAATAAGTAT TGGACAACTT 
GTTAGTCTTC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCCTT GGCACATTTT 
ATATGGATTG CTCCTTTACA AGTGACTCTT CTGATGGGGC TTCTCTGGGA CTTGTTACAG 
TTCTCAGCCT TCTGTGGCCT TGGTTTACTG ATAATCCTGG TTATTTTTCA AGCTATCCTA 
GGGAAGATGA TGGTGAAGTA CAGAGATCAG AGAGCTGCAA AGATCAATGA AAGACTCGTG 
ATCACATCAG AAATTATTGA TAATATCTAT TCTGTTAAGG CATATTGTTG GGAATCAGCG 
ATGGAGAAAA TGATTGAAAA CTTGAGAGAG GTGGAGCTGA AAATGACCCG GAAGGCGGCC 
TATATGAGGT TCTTCACTAG CTCTGCCTTC TTCTTTTCAG GGTTCTTTGT AGTCTTTCTA 
TCTGTGCTTC CCTACACAGT CATCAACGGA ATCGTCCTAC GAAAAATATT CACAACCATT 
TCATTCTGCA TTGTCCTACG TATGTCAGTC ACACGGCAGT TCCCCACTGC CGTACAGATA 
TGGTATGATT CTTTTGGAAT GATAAGAAAA ATACAGGATT TCCTGCAGAA ACAAGAGTAT 
AAAGTACTGG AGTATAACTT AATGACCACA GGCATAATCA TGGAAAATGT AACAGCATTT 
TGGGAGGAGG GATTTGGGGA ATTACTGGAG AAAGTACAAC AAAGCAATGG TGACAGAAAA 
CATTCCAGTG ATGAGAACAA TGTCAGTTTC AGTCATCTCT GCCTTGTGGG AAATCCTGTG 
CTGAAAAACA TCAATTTGAA TATAGAGAAA GGAGAGATGT TGGCTATTAC TGGATCTACT 
GGATCAGGAA AGACATCACT CCTGATGTTG ATTTTGGGAG AACTGGAAGC TTCAGAGGGA 
ATTATTAAGC ACAGTGGAAG AGTTTCATTC TGCTCTCAAT TTTCTTGGAT TATGCCGGGT 
ACTATCAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AGTACAGATA TAAGAGTGTT 
GTCAAAGCTT GCCAACTACA GCAGGACATC ACCAAGTTTG CAGAACAAGA CAACACAGTT 
CTTGGAGAAG GTGGAGTCAC ACTGAGTGGA GGTCAGCGTG CAAGGATTTC TTTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTGTACCTA TTAGATTCCC CTTTTGGATA TCTAGATGTT 
TTTACTGAAG AACAAGTATT TGAAAGCTGT GTTTGTAAAT TGATGGCCAA CAAAACTAGG 
ATTTTGGTTA CATCTAAAAT GGAACACTTA AGGAAAGCTG ACAAAATACT AATTTTGCAT 
CAGGGCAGTA GCTATTTTTA TGGGACATTT TCTGAGCTAC AAAGTCTACG TCCAGACTTC 
AGTTCGAAAC TCATGGGGTA TGATACTTTT GACCAGTTTA CTGAGGAAAG AAGAAGTTCA 
ATTCTAACTG AGACCTTACG CAGGTTCTCA GTAGACGATT CCTCTGCCCC GTGGAGCAAA 
CCCAAACAGT CGTTTAGACA GACTGGAGAG GTGGGAGAAA AAAGGAAGAA CTCTATTCTA 
AATTCATTCA GCTCTGTAAG GAAAATTTCC ATTGTGCAAA AGACTCCATT ATGTATCGAT 
GGAGAGTCTG ATGATCTCCA AGAAAAGAGA CTGTCCCTAG TTCCGGATTC TGAACAGGGG 
GAGGCTGCTC TGCCGCGCAG CAACATGATC GCCACCGGCC CCACATTTCC AGGCAGAAGA 
AGACAGTCTG TTTTGGATCT GATGACGTTC ACACCCAACT CAGGCTCCAG CAATCTTCAG 
AGGACCAGAA CTTCTATTCG AAAAATCTCC TTAGTCCCTC AGATAAGCTT AAATGAAGTG 
GATGTATATT CAAGGAGATT ATCGCAAGAT AGCACACTGA ACATCACTGA AGAAATTAAC 
GAAGAAGATT TAAAGGAGTG TTTTCTTGAT GATGTGATCA AGATACCCCC GGTGACAACA 
TGGAACACAT ACCTACGATA TTTTACTCTC CATAAAGGCT TACTGCTAGT GCTGATTTGG 
TGCGTACTGG TTTTTCTGGT TGAGGTGGCT GCTTCTTTAT TTGTGTTATG GTTGCTTAAA 
AACAACCCTG TTAACAGTGG AAACAATGGT ACTAAAATTT CCAATAGCTC CTATGTTGTG 
ATCATCACCA GTACCAGTTT CTATTATATT TTTTACATTT ACGTGGGAGT GGCTGACACT 
TTGCTTGCCC TGAGCCTCTT CAGAGGTTTG CCGCTGGTGC ATACGTTAAT CACAGCATCA 
AAAATTTTGC ACAGGAAAAT GTTACACTCC ATTCTTCACG CCCCTATGTC GACCATCAGC 
AAGCTGAAAG CAGGTGGGAT TCTTAACAGA TTCTCCAAAG ATATAGCAAT TTTGGATGAC 
TTTCTGCCTC TTACCATTTT TGACTTCATT CAGTTGGTGT TCATTGTGAT TGGAGCTATA 
ATAGTCGTCT CGGCATTACA ACCCTACATC TTCCTAGCAA CGGTGCCAGG GCTAGTAGTC 
TTTATTTTAC TGAGGGCCTA CTTCCTTCAT ACAGCACAGC AGCTCAAACA ACTGGAATCT 
GAAGGCAGGA GTCCAATTTT CACCCACCTT GTGACAAGCT TAAAAGGACT CTGGACACTT 
CGAGCCTTCC GACGCCAGAC TTACTTTGAA ACTCTGTTCC ACAAAGCTCT GAATTTGCAC 
ACTGCCAACT GGTTTATGTA TCTGGCAACC TTGCGCTGGT TCCAAATGAG AATAGACATG 
ATATTTGTCC TCTTCTTCAT TGTTGTTACC TTCATCTCCA TTTTAACAAC AGGTGAAGGA 
GAAGGAACAG CTGGTATTAT TCTAACTTTA GCTATGAATA TCATGAGTAC TTTGCAGTGG 
GCTGTGAACT CAAGCATTGA TACAGATAGC TTGGTGGGGC TCTTAGGAAG AACTGGATCA 
GGAAAAAGTA CTTTGCTTTC AGCATTTTTA CGAATGTTGA ACATTAAAGG TGATATAGAG 
ATTGATGGTG TCTCATGGAA TTCAGTGACC TTACAAGAAT GGAGGAAAGC TTTCGGAGTG 
ATAACACAGA AAGTATTTAT CTTTTCTGGA ACATTCAGAC AAAACCTGGA TCCCAATGGA 
AAATGGAAAG ATGAAGAAAT ATGGAAAGTT GCAGATGAGG TTGGACTCAA GTCTGTAATA 
GAGCAGTTTC CTGGACAGCT CAACTTTACC CTTGTGGATG GGGGTTATGT GCTAAGCCAT 
GGCCATAAGC AATTAATGTG CTTGGCCCGA TCAGTTCTCA GTAAGGCCAA GATCATACTG 
CTTGATGAGC CCAGTGCCCA TCTAGACCCC ATAACATACC AAGTCATTCG ACGAGTTCTA 
AAACAAGCCT TCGCTGGTTG CACAGTCATC CTCTGTGAAC ACAGGATAGA AGCGATGTTG 
GATTGCCAGC GATTTTTGGT CATAGAAGAG AGCAATGTCT GGCAGTACGA CTCCCTTCAG 
GCACTTCTGA GTGAGAAGAG TATCTTCCAG CAGGCCATTA GCTCCTCGGA AAAGATGAGG 
TTCTTCCAGG GCCGCCACTC CAGCAAGCAC AAGCCTCGGA CGCAAATTAC TGCTCTGAAA 
GAGGAGACAG AAGAAGAAGT TCAAGAAACC CGTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505042.1
CDS	259..4437
Translation	MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSADSADHLSEKLEREWDREQASKKNPQLIHALRRCFFWRFLFYGILLYLGEVTKAVQPVLLGRIIASYDPENKVERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHRIGMQMRTAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFIWIAPLQVTLLMGLLWDLLQFSAFCGLGLLIILVIFQAILGKMMVKYRDQRAAKINERLVITSEIIDNIYSVKAYCWESAMEKMIENLREVELKMTRKAAYMRFFTSSAFFFSGFFVVFLSVLPYTVINGIVLRKIFTTISFCIVLRMSVTRQFPTAVQIWYDSFGMIRKIQDFLQKQEYKVLEYNLMTTGIIMENVTAFWEEGFGELLEKVQQSNGDRKHSSDENNVSFSHLCLVGNPVLKNINLNIEKGEMLAITGSTGSGKTSLLMLILGELEASEGIIKHSGRVSFCSQFSWIMPGTIKENIIFGVSYDEYRYKSVVKACQLQQDITKFAEQDNTVLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVFTEEQVFESCVCKLMANKTRILVTSKMEHLRKADKILILHQGSSYFYGTFSELQSLRPDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDSSAPWSKPKQSFRQTGEVGEKRKNSILNSFSSVRKISIVQKTPLCIDGESDDLQEKRLSLVPDSEQGEAALPRSNMIATGPTFPGRRRQSVLDLMTFTPNSGSSNLQRTRTSIRKISLVPQISLNEVDVYSRRLSQDSTLNITEEINEEDLKECFLDDVIKIPPVTTWNTYLRYFTLHKGLLLVLIWCVLVFLVEVAASLFVLWLLKNNPVNSGNNGTKISNSSYVVIITSTSFYYIFYIYVGVADTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMSTISKLKAGGILNRFSKDIAILDDFLPLTIFDFIQLVFIVIGAIIVVSALQPYIFLATVPGLVVFILLRAYFLHTAQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYLATLRWFQMRIDMIFVLFFIVVTFISILTTGEGEGTAGIILTLAMNIMSTLQWAVNSSIDTDSLVGLLGRTGSGKSTLLSAFLRMLNIKGDIEIDGVSWNSVTLQEWRKAFGVITQKVFIFSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLNFTLVDGGYVLSHGHKQLMCLARSVLSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCEHRIEAMLDCQRFLVIEESNVWQYDSLQALLSEKSIFQQAISSSEKMRFFQGRHSSKHKPRTQITALKEETEEEVQETRL

Target ORF information:

RefSeq Version	XM_006504979.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504979.3

ORF Insert Sequence:

ATGCAGAAGT CGCCTTTGGA GAAAGCCAGC TTTATCTCCA AACTCTTCTT CAGCTGGACC 
ACACCAATTT TGAGGAAAGG GTACAGACAC CACTTGGAGT TGTCAGACAT ATACCAAGCC 
CCTTCTGCTG ATTCAGCTGA CCACTTGTCT GAAAAACTAG AAAGAGAATG GGACAGAGAA 
CAAGCTTCAA AAAAGAATCC CCAGCTTATC CACGCCCTTC GGCGATGCTT TTTCTGGAGA 
TTCCTCTTCT ATGGAATTTT GCTATACCTA GGGGAAGTCA CCAAGGCTGT CCAGCCTGTC 
TTGCTAGGAA GAATCATAGC ATCCTATGAT CCAGAAAACA AGGTGGAACG TTCCATTGCC 
ATTTACCTTG GCATAGGCTT ATGCCTTCTC TTCATTGTCA GGACACTGCT TCTTCACCCA 
GCTATTTTTG GCCTTCATCG CATTGGAATG CAGATGAGAA CAGCTATGTT TAGCTTGATT 
TATAAGAAGA CTTTAAAGTT GTCAAGCCGC GTTCTTGATA AAATAAGTAT TGGACAACTT 
GTTAGTCTTC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCCTT GGCACATTTT 
ATATGGATTG CTCCTTTACA AGTGACTCTT CTGATGGGGC TTCTCTGGGA CTTGTTACAG 
TTCTCAGCCT TCTGTGGCCT TGGTTTACTG ATAATCCTGG TTATTTTTCA AGCTATCCTA 
GGGAAGATGA TGGTGAAGTA CAGAGATCAG AGAGCTGCAA AGATCAATGA AAGACTCGTG 
ATCACATCAG AAATTATTGA TAATATCTAT TCTGTTAAGG CATATTGTTG GGAATCAGCG 
ATGGAGAAAA TGATTGAAAA CTTGAGAGAG GTGGAGCTGA AAATGACCCG GAAGGCGGCC 
TATATGAGGT TCTTCACTAG CTCTGCCTTC TTCTTTTCAG GGTTCTTTGT AGTCTTTCTA 
TCTGTGCTTC CCTACACAGT CATCAACGGA ATCGTCCTAC GAAAAATATT CACAACCATT 
TCATTCTGCA TTGTCCTACG TATGTCAGTC ACACGGCAGT TCCCCACTGC CGTACAGATA 
TGGTATGATT CTTTTGGAAT GATAAGAAAA ATACAGGATT TCCTGCAGAA ACAAGAGTAT 
AAAGTACTGG AGTATAACTT AATGACCACA GGCATAATCA TGGAAAATGT AACAGCATTT 
TGGGAGGAGG GATTTGGGGA ATTACTGGAG AAAGTACAAC AAAGCAATGG TGACAGAAAA 
CATTCCAGTG ATGAGAACAA TGTCAGTTTC AGTCATCTCT GCCTTGTGGG AAATCCTGTG 
CTGAAAAACA TCAATTTGAA TATAGAGAAA GGAGAGATGT TGGCTATTAC TGGATCTACT 
GGATCAGGAA AGACATCACT CCTGATGTTG ATTTTGGGAG AACTGGAAGC TTCAGAGGGA 
ATTATTAAGC ACAGTGGAAG AGTTTCATTC TGCTCTCAAT TTTCTTGGAT TATGCCGGGT 
ACTATCAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AGTACAGATA TAAGAGTGTT 
GTCAAAGCTT GCCAACTACA GCAGGACATC ACCAAGTTTG CAGAACAAGA CAACACAGTT 
CTTGGAGAAG GTGGAGTCAC ACTGAGTGGA GGTCAGCGTG CAAGGATTTC TTTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTGTACCTA TTAGATTCCC CTTTTGGATA TCTAGATGTT 
TTTACTGAAG AACAAGTATT TGAAAGCTGT GTTTGTAAAT TGATGGCCAA CAAAACTAGG 
ATTTTGGTTA CATCTAAAAT GGAACACTTA AGGAAAGCTG ACAAAATACT AATTTTGCAT 
CAGGGCAGTA GCTATTTTTA TGGGACATTT TCTGAGCTAC AAAGTCTACG TCCAGACTTC 
AGTTCGAAAC TCATGGGGTA TGATACTTTT GACCAGTTTA CTGAGGAAAG AAGAAGTTCA 
ATTCTAACTG AGACCTTACG CAGGTTCTCA GTAGACGATT CCTCTGCCCC GTGGAGCAAA 
CCCAAACAGT CGTTTAGACA GACTGGAGAG GTGGGAGAAA AAAGGAAGAA CTCTATTCTA 
AATTCATTCA GCTCTGTAAG GAAAATTTCC ATTGTGCAAA AGACTCCATT ATGTATCGAT 
GGAGAGTCTG ATGATCTCCA AGAAAAGAGA CTGTCCCTAG TTCCGGATTC TGAACAGGGG 
GAGGCTGCTC TGCCGCGCAG CAACATGATC GCCACCGGCC CCACATTTCC AGGCAGAAGA 
AGACAGTCTG TTTTGGATCT GATGACGTTC ACACCCAACT CAGGCTCCAG CAATCTTCAG 
AGGACCAGAA CTTCTATTCG AAAAATCTCC TTAGTCCCTC AGATAAGCTT AAATGAAGTG 
GATGTATATT CAAGGAGATT ATCGCAAGAT AGCACACTGA ACATCACTGA AGAAATTAAC 
GAAGAAGATT TAAAGGAGTG TTTTCTTGAT GATGTGATCA AGATACCCCC GGTGACAACA 
TGGAACACAT ACCTACGATA TTTTACTCTC CATAAAGGCT TACTGCTAGT GCTGATTTGG 
TGCGTACTGG TTTTTCTGGT TGAGGTGGCT GCTTCTTTAT TTGTGTTATG GTTGCTTAAA 
AACAACCCTG TTAACAGTGG AAACAATGGT ACTAAAATTT CCAATAGCTC CTATGTTGTG 
ATCATCACCA GTACCAGTTT CTATTATATT TTTTACATTT ACGTGGGAGT GGCTGACACT 
TTGCTTGCCC TGAGCCTCTT CAGAGGTTTG CCGCTGGTGC ATACGTTAAT CACAGCATCA 
AAAATTTTGC ACAGGAAAAT GTTACACTCC ATTCTTCACG CCCCTATGTC GACCATCAGC 
AAGCTGAAAG CAGGTGGGAT TCTTAACAGA TTCTCCAAAG ATATAGCAAT TTTGGATGAC 
TTTCTGCCTC TTACCATTTT TGACTTCATT CAGTTGGTGT TCATTGTGAT TGGAGCTATA 
ATAGTCGTCT CGGCATTACA ACCCTACATC TTCCTAGCAA CGGTGCCAGG GCTAGTAGTC 
TTTATTTTAC TGAGGGCCTA CTTCCTTCAT ACAGCACAGC AGCTCAAACA ACTGGAATCT 
GAAGGCAGGA GTCCAATTTT CACCCACCTT GTGACAAGCT TAAAAGGACT CTGGACACTT 
CGAGCCTTCC GACGCCAGAC TTACTTTGAA ACTCTGTTCC ACAAAGCTCT GAATTTGCAC 
ACTGCCAACT GGTTTATGTA TCTGGCAACC TTGCGCTGGT TCCAAATGAG AATAGACATG 
ATATTTGTCC TCTTCTTCAT TGTTGTTACC TTCATCTCCA TTTTAACAAC AGGTGAAGGA 
GAAGGAACAG CTGGTATTAT TCTAACTTTA GCTATGAATA TCATGAGTAC TTTGCAGTGG 
GCTGTGAACT CAAGCATTGA TACAGATAGC TTGGTGGGGC TCTTAGGAAG AACTGGATCA 
GGAAAAAGTA CTTTGCTTTC AGCATTTTTA CGAATGTTGA ACATTAAAGG TGATATAGAG 
ATTGATGGTG TCTCATGGAA TTCAGTGACC TTACAAGAAT GGAGGAAAGC TTTCGGAGTG 
ATAACACAGA AAGTATTTAT CTTTTCTGGA ACATTCAGAC AAAACCTGGA TCCCAATGGA 
AAATGGAAAG ATGAAGAAAT ATGGAAAGTT GCAGATGAGG TTGGACTCAA GTCTGTAATA 
GAGCAGTTTC CTGGACAGCT CAACTTTACC CTTGTGGATG GGGGTTATGT GCTAAGCCAT 
GGCCATAAGC AATTAATGTG CTTGGCCCGA TCAGTTCTCA GTAAGGCCAA GATCATACTG 
CTTGATGAGC CCAGTGCCCA TCTAGACCCC ATAACATACC AAGTCATTCG ACGAGTTCTA 
AAACAAGCCT TCGCTGGTTG CACAGTCATC CTCTGTGAAC ACAGGATAGA AGCGATGTTG 
GATTGCCAGC GATTTTTGGT CATAGAAGAG AGCAATGTCT GGCAGTACGA CTCCCTTCAG 
GCACTTCTGA GTGAGAAGAG TATCTTCCAG CAGGCCATTA GCTCCTCGGA AAAGATGAGG 
TTCTTCCAGG GCCGCCACTC CAGCAAGCAC AAGCCTCGGA CGCAAATTAC TGCTCTGAAA 
GAGGAGACAG AAGAAGAAGT TCAAGAAACC CGTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Cftr cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

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