Cfi cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cfi
Entrez Gene ID	12630
Full Name	complement component factor i
General protein information	Preferred Names complement component factor i Names complement factor I C3B/C4B inactivator
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 G3\|3 59.21 cM
Summary	This gene encodes a serine protease that plays an important role in the classical and alternative complement pathways where it cleaves C4b and C3b components of C3 and C5 convertases. The encoded preproprotein undergoes proteolytic processing to generate an active, disulfide-linked heterodimeric enzyme comprised of heavy and light chains. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006500962.2	XP_006501025.1	complement factor I isoform X2
XM_006500963.3	XP_006501026.1	complement factor I isoform X2
NM_001329552.1	NP_001316481.1	complement factor I isoform 2 precursor
XM_006500962.3	XP_006501025.1	complement factor I isoform X1
XM_006500963.2	XP_006501026.1	complement factor I isoform X3
NM_007686.3	NP_031712.2	complement factor I isoform 1 preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cfi	NP_077071.1
Bos taurus (cattle)	CFI	NP_001033185.1
Gallus gallus (chicken)	CFI	NP_001258947.1
Xenopus tropicalis (tropical clawed frog)	cfi	NP_001027508.1
Pan troglodytes (chimpanzee)	CFI	XP_003310493.1
Mus musculus (house mouse)	Cfi	NP_031712.2
Canis lupus familiaris (dog)	CFI	XP_005639326.1
Homo sapiens (human)	CFI	NP_000195.2
Danio rerio (zebrafish)	cfi	XP_002667431.3

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

C3 opsonization regulates endocytic handling of apoptotic cells resulting in enhanced T-cell responses to cargo-derived antigens.
Baudino L, Sardini A, Ruseva MM, Fossati-Jimack L, Cook HT, Scott D, Simpson E, Botto M
Proceedings of the National Academy of Sciences of the United States of America111(4)1503-8(2014 Jan)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Lumayag S, Haldin CE, Corbett NJ, Wahlin KJ, Cowan C, Turturro S, Larsen PE, Kovacs B, Witmer PD, Valle D, Zack DJ, Nicholson DA, Xu S
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Factor I-mediated generation of activated C3 fragments in the circulation is a critical determinant for the development of MPGN2 associated with factor H deficiency.
Title: Factor I is required for the development of membranoproliferative glomerulonephritis in factor H-deficient mice.

The following Cfi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cfi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22367	NM_007686.3 Latest version!	Mus musculus complement component factor i (Cfi), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu31594	NM_001329552.1 Latest version!	Mus musculus complement component factor i (Cfi), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu31595	XM_006500962.3 Latest version!	Mus musculus complement component factor i (Cfi), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu31596	XM_006500963.3 Latest version!	Mus musculus complement component factor i (Cfi), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu31595	XM_006500962.2	Mus musculus complement component factor i (Cfi), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu31596	XM_006500963.2	Mus musculus complement component factor i (Cfi), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cfi ( NM_007686.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031712.2 Mus musculus complement component factor i (Cfi), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22367
Clone ID Related Accession (Same CDS sequence)	NM_007686.3
Accession Version	NM_007686.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	complement factor I isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC111097.10, CJ133989.1, AI326560.1, CN691735.1, AA530545.1 and BG072327.1. On Jul 16, 2016 this sequence version replaced NM_007686.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U47810.1, SRR1660815.138734.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAA 
GACCGACAGC AGAACATTCC AAAAGGCCTT GCACGGTCAG CTCAAGGAGA AGCTGAAATT 
GAAACTGAAG AAACAGAAAT GTTGACTCCT GGTATGGACA ATGAAAGAAA ACGGATAAAG 
TCCTTATTAC CTAAACTCTC CTGTGGAGTC AAAAGAAACA CTCACACTCG CAGGAAACGA 
GTGATCGGAG GGAAGCCAGC GAATGTGGGA GACTACCCAT GGCAGGTGGC AATTAAGGAT 
GGCCAAAGAA TCACCTGTGG TGGCATTTAT ATCGGTGGCT GTTGGATTCT GACTGCTGCG 
CACTGTGTCA GACCCAGTAG AGCTCACAGT TACCAAGTCT GGACGGCTTT ATTAGACTGG 
CTAAAACCTA ACTCTCAGTT GGGAATTCAA ACGGTGAAGA GAGTTATTGT TCACGAGAAA 
TATAATGGAG CCACCTTCCA AAATGACATA GCTTTGATTG AAATGAAAAT GCACACGGGC 
AAGAAAGAAT GTGAACTCCC CAATTCCGTC CCTGCCTGCG TCCCGTGGTC TCCATATCTG 
TTCCAACCGA ATGACAGATG TATCATCTCT GGATGGGGTC GAGGGAAAGA TAACCAAAAA 
GTCTACTCAC TCAGGTGGGG CGAAGTTGAT CTAATAGGCA ACTGCTCTCA GTTTTACCCA 
GATCGCTACT ATGAGAAAGA GATGCAGTGC GCAGGTACGC GTGATGGGTC CATTGATGCC 
TGCAAAGGAG ACTCCGGAGG CCCCTTGGTC TGTGAGGATA TCAACAATGT CACTTACGTT 
TGGGGCATTG TGAGCTGGGG AGAAAATTGT GGGAAGCCCG AGTTCCCAGG TGTTTACACC 
AGAGTGGCCA ATTATTTTGA TTGGATTAGC TATCACGTAG GAAGGTCTCT TGTTTCTCAA 
CACAATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031712.2
CDS	72..1883
Translation	MKLAHLSLFLLALHLSSSRSPSASDLPQEELVDQKCLLQKYTHRSCNKVFCQPWQRCIEGTCICKLPYQCPRAGTPVCAMNGRSYPTYCHQKSFECLHPEIKFSHNGTCAAEGKFNVSLIYGRTKTEGLVQVKLVDQDERMFICKNSWSMAEANVACVDLGFPLGVRDIQGSFNISGNLHINDTECLHVHCRGVETSLAECAFTKRRTELSNGLAGVVCYKQDADFPTSLSFQCVNGKHIPQEKACNGVNDCGDQSDELCCKGCRGNASLCKSGVCIPDQYKCNGEVDCITGEDESRCEEDRQQNIPKGLARSAQGEAEIETEETEMLTPGMDNERKRIKSLLPKLSCGVKRNTHTRRKRVIGGKPANVGDYPWQVAIKDGQRITCGGIYIGGCWILTAAHCVRPSRAHSYQVWTALLDWLKPNSQLGIQTVKRVIVHEKYNGATFQNDIALIEMKMHTGKKECELPNSVPACVPWSPYLFQPNDRCIISGWGRGKDNQKVYSLRWGEVDLIGNCSQFYPDRYYEKEMQCAGTRDGSIDACKGDSGGPLVCEDINNVTYVWGIVSWGENCGKPEFPGVYTRVANYFDWISYHVGRSLVSQHNV

Target ORF information:

RefSeq Version	NM_007686.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component factor i (Cfi), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007686.3

ORF Insert Sequence:

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAA 
GACCGACAGC AGAACATTCC AAAAGGCCTT GCACGGTCAG CTCAAGGAGA AGCTGAAATT 
GAAACTGAAG AAACAGAAAT GTTGACTCCT GGTATGGACA ATGAAAGAAA ACGGATAAAG 
TCCTTATTAC CTAAACTCTC CTGTGGAGTC AAAAGAAACA CTCACACTCG CAGGAAACGA 
GTGATCGGAG GGAAGCCAGC GAATGTGGGA GACTACCCAT GGCAGGTGGC AATTAAGGAT 
GGCCAAAGAA TCACCTGTGG TGGCATTTAT ATCGGTGGCT GTTGGATTCT GACTGCTGCG 
CACTGTGTCA GACCCAGTAG AGCTCACAGT TACCAAGTCT GGACGGCTTT ATTAGACTGG 
CTAAAACCTA ACTCTCAGTT GGGAATTCAA ACGGTGAAGA GAGTTATTGT TCACGAGAAA 
TATAATGGAG CCACCTTCCA AAATGACATA GCTTTGATTG AAATGAAAAT GCACACGGGC 
AAGAAAGAAT GTGAACTCCC CAATTCCGTC CCTGCCTGCG TCCCGTGGTC TCCATATCTG 
TTCCAACCGA ATGACAGATG TATCATCTCT GGATGGGGTC GAGGGAAAGA TAACCAAAAA 
GTCTACTCAC TCAGGTGGGG CGAAGTTGAT CTAATAGGCA ACTGCTCTCA GTTTTACCCA 
GATCGCTACT ATGAGAAAGA GATGCAGTGC GCAGGTACGC GTGATGGGTC CATTGATGCC 
TGCAAAGGAG ACTCCGGAGG CCCCTTGGTC TGTGAGGATA TCAACAATGT CACTTACGTT 
TGGGGCATTG TGAGCTGGGG AGAAAATTGT GGGAAGCCCG AGTTCCCAGG TGTTTACACC 
AGAGTGGCCA ATTATTTTGA TTGGATTAGC TATCACGTAG GAAGGTCTCT TGTTTCTCAA 
CACAATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cfi ( NM_001329552.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001316481.1 Mus musculus complement component factor i (Cfi), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu31594
Clone ID Related Accession (Same CDS sequence)	NM_001329552.1
Accession Version	NM_001329552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	complement factor I isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC111097.10, CJ133989.1, CN691735.1, AW110859.1 and AA920214.1. On Jul 17, 2016 this sequence version replaced XM_006500961.2. Transcript Variant: This variant (2) uses an in-frame alternate splice site in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.41327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAC 
CGACAGCAGA ACATTCCAAA AGGCCTTGCA CGGTCAGCTC AAGGAGAAGC TGAAATTGAA 
ACTGAAGAAA CAGAAATGTT GACTCCTGGT ATGGACAATG AAAGAAAACG GATAAAGTCC 
TTATTACCTA AACTCTCCTG TGGAGTCAAA AGAAACACTC ACACTCGCAG GAAACGAGTG 
ATCGGAGGGA AGCCAGCGAA TGTGGGAGAC TACCCATGGC AGGTGGCAAT TAAGGATGGC 
CAAAGAATCA CCTGTGGTGG CATTTATATC GGTGGCTGTT GGATTCTGAC TGCTGCGCAC 
TGTGTCAGAC CCAGTAGAGC TCACAGTTAC CAAGTCTGGA CGGCTTTATT AGACTGGCTA 
AAACCTAACT CTCAGTTGGG AATTCAAACG GTGAAGAGAG TTATTGTTCA CGAGAAATAT 
AATGGAGCCA CCTTCCAAAA TGACATAGCT TTGATTGAAA TGAAAATGCA CACGGGCAAG 
AAAGAATGTG AACTCCCCAA TTCCGTCCCT GCCTGCGTCC CGTGGTCTCC ATATCTGTTC 
CAACCGAATG ACAGATGTAT CATCTCTGGA TGGGGTCGAG GGAAAGATAA CCAAAAAGTC 
TACTCACTCA GGTGGGGCGA AGTTGATCTA ATAGGCAACT GCTCTCAGTT TTACCCAGAT 
CGCTACTATG AGAAAGAGAT GCAGTGCGCA GGTACGCGTG ATGGGTCCAT TGATGCCTGC 
AAAGGAGACT CCGGAGGCCC CTTGGTCTGT GAGGATATCA ACAATGTCAC TTACGTTTGG 
GGCATTGTGA GCTGGGGAGA AAATTGTGGG AAGCCCGAGT TCCCAGGTGT TTACACCAGA 
GTGGCCAATT ATTTTGATTG GATTAGCTAT CACGTAGGAA GGTCTCTTGT TTCTCAACAC 
AATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316481.1
CDS	72..1880
Translation	MKLAHLSLFLLALHLSSSRSPSASDLPQEELVDQKCLLQKYTHRSCNKVFCQPWQRCIEGTCICKLPYQCPRAGTPVCAMNGRSYPTYCHQKSFECLHPEIKFSHNGTCAAEGKFNVSLIYGRTKTEGLVQVKLVDQDERMFICKNSWSMAEANVACVDLGFPLGVRDIQGSFNISGNLHINDTECLHVHCRGVETSLAECAFTKRRTELSNGLAGVVCYKQDADFPTSLSFQCVNGKHIPQEKACNGVNDCGDQSDELCCKGCRGNASLCKSGVCIPDQYKCNGEVDCITGEDESRCEDRQQNIPKGLARSAQGEAEIETEETEMLTPGMDNERKRIKSLLPKLSCGVKRNTHTRRKRVIGGKPANVGDYPWQVAIKDGQRITCGGIYIGGCWILTAAHCVRPSRAHSYQVWTALLDWLKPNSQLGIQTVKRVIVHEKYNGATFQNDIALIEMKMHTGKKECELPNSVPACVPWSPYLFQPNDRCIISGWGRGKDNQKVYSLRWGEVDLIGNCSQFYPDRYYEKEMQCAGTRDGSIDACKGDSGGPLVCEDINNVTYVWGIVSWGENCGKPEFPGVYTRVANYFDWISYHVGRSLVSQHNV

Target ORF information:

RefSeq Version	NM_001329552.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component factor i (Cfi), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329552.1

ORF Insert Sequence:

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAC 
CGACAGCAGA ACATTCCAAA AGGCCTTGCA CGGTCAGCTC AAGGAGAAGC TGAAATTGAA 
ACTGAAGAAA CAGAAATGTT GACTCCTGGT ATGGACAATG AAAGAAAACG GATAAAGTCC 
TTATTACCTA AACTCTCCTG TGGAGTCAAA AGAAACACTC ACACTCGCAG GAAACGAGTG 
ATCGGAGGGA AGCCAGCGAA TGTGGGAGAC TACCCATGGC AGGTGGCAAT TAAGGATGGC 
CAAAGAATCA CCTGTGGTGG CATTTATATC GGTGGCTGTT GGATTCTGAC TGCTGCGCAC 
TGTGTCAGAC CCAGTAGAGC TCACAGTTAC CAAGTCTGGA CGGCTTTATT AGACTGGCTA 
AAACCTAACT CTCAGTTGGG AATTCAAACG GTGAAGAGAG TTATTGTTCA CGAGAAATAT 
AATGGAGCCA CCTTCCAAAA TGACATAGCT TTGATTGAAA TGAAAATGCA CACGGGCAAG 
AAAGAATGTG AACTCCCCAA TTCCGTCCCT GCCTGCGTCC CGTGGTCTCC ATATCTGTTC 
CAACCGAATG ACAGATGTAT CATCTCTGGA TGGGGTCGAG GGAAAGATAA CCAAAAAGTC 
TACTCACTCA GGTGGGGCGA AGTTGATCTA ATAGGCAACT GCTCTCAGTT TTACCCAGAT 
CGCTACTATG AGAAAGAGAT GCAGTGCGCA GGTACGCGTG ATGGGTCCAT TGATGCCTGC 
AAAGGAGACT CCGGAGGCCC CTTGGTCTGT GAGGATATCA ACAATGTCAC TTACGTTTGG 
GGCATTGTGA GCTGGGGAGA AAATTGTGGG AAGCCCGAGT TCCCAGGTGT TTACACCAGA 
GTGGCCAATT ATTTTGATTG GATTAGCTAT CACGTAGGAA GGTCTCTTGT TTCTCAACAC 
AATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cfi ( XM_006500962.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501025.1 Mus musculus complement component factor i (Cfi), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu31595
Clone ID Related Accession (Same CDS sequence)	XM_006500962.2 , XM_006500962.3
Accession Version	XM_006500962.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	complement factor I isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500962.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAA 
GACCGACAGC AGAACATTCC AAAAGGAGAA GCTGAAATTG AAACTGAAGA AACAGAAATG 
TTGACTCCTG GTATGGACAA TGAAAGAAAA CGGATAAAGT CCTTATTACC TAAACTCTCC 
TGTGGAGTCA AAAGAAACAC TCACACTCGC AGGAAACGAG TGATCGGAGG GAAGCCAGCG 
AATGTGGGAG ACTACCCATG GCAGGTGGCA ATTAAGGATG GCCAAAGAAT CACCTGTGGT 
GGCATTTATA TCGGTGGCTG TTGGATTCTG ACTGCTGCGC ACTGTGTCAG ACCCAGTAGA 
GCTCACAGTT ACCAAGTCTG GACGGCTTTA TTAGACTGGC TAAAACCTAA CTCTCAGTTG 
GGAATTCAAA CGGTGAAGAG AGTTATTGTT CACGAGAAAT ATAATGGAGC CACCTTCCAA 
AATGACATAG CTTTGATTGA AATGAAAATG CACACGGGCA AGAAAGAATG TGAACTCCCC 
AATTCCGTCC CTGCCTGCGT CCCGTGGTCT CCATATCTGT TCCAACCGAA TGACAGATGT 
ATCATCTCTG GATGGGGTCG AGGGAAAGAT AACCAAAAAG TCTACTCACT CAGGTGGGGC 
GAAGTTGATC TAATAGGCAA CTGCTCTCAG TTTTACCCAG ATCGCTACTA TGAGAAAGAG 
ATGCAGTGCG CAGGTACGCG TGATGGGTCC ATTGATGCCT GCAAAGGAGA CTCCGGAGGC 
CCCTTGGTCT GTGAGGATAT CAACAATGTC ACTTACGTTT GGGGCATTGT GAGCTGGGGA 
GAAAATTGTG GGAAGCCCGA GTTCCCAGGT GTTTACACCA GAGTGGCCAA TTATTTTGAT 
TGGATTAGCT ATCACGTAGG AAGGTCTCTT GTTTCTCAAC ACAATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501025.1
CDS	83..1873
Translation	MKLAHLSLFLLALHLSSSRSPSASDLPQEELVDQKCLLQKYTHRSCNKVFCQPWQRCIEGTCICKLPYQCPRAGTPVCAMNGRSYPTYCHQKSFECLHPEIKFSHNGTCAAEGKFNVSLIYGRTKTEGLVQVKLVDQDERMFICKNSWSMAEANVACVDLGFPLGVRDIQGSFNISGNLHINDTECLHVHCRGVETSLAECAFTKRRTELSNGLAGVVCYKQDADFPTSLSFQCVNGKHIPQEKACNGVNDCGDQSDELCCKGCRGNASLCKSGVCIPDQYKCNGEVDCITGEDESRCEEDRQQNIPKGEAEIETEETEMLTPGMDNERKRIKSLLPKLSCGVKRNTHTRRKRVIGGKPANVGDYPWQVAIKDGQRITCGGIYIGGCWILTAAHCVRPSRAHSYQVWTALLDWLKPNSQLGIQTVKRVIVHEKYNGATFQNDIALIEMKMHTGKKECELPNSVPACVPWSPYLFQPNDRCIISGWGRGKDNQKVYSLRWGEVDLIGNCSQFYPDRYYEKEMQCAGTRDGSIDACKGDSGGPLVCEDINNVTYVWGIVSWGENCGKPEFPGVYTRVANYFDWISYHVGRSLVSQHNV

Target ORF information:

RefSeq Version	XM_006500962.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component factor i (Cfi), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500962.2

ORF Insert Sequence:

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAA 
GACCGACAGC AGAACATTCC AAAAGGAGAA GCTGAAATTG AAACTGAAGA AACAGAAATG 
TTGACTCCTG GTATGGACAA TGAAAGAAAA CGGATAAAGT CCTTATTACC TAAACTCTCC 
TGTGGAGTCA AAAGAAACAC TCACACTCGC AGGAAACGAG TGATCGGAGG GAAGCCAGCG 
AATGTGGGAG ACTACCCATG GCAGGTGGCA ATTAAGGATG GCCAAAGAAT CACCTGTGGT 
GGCATTTATA TCGGTGGCTG TTGGATTCTG ACTGCTGCGC ACTGTGTCAG ACCCAGTAGA 
GCTCACAGTT ACCAAGTCTG GACGGCTTTA TTAGACTGGC TAAAACCTAA CTCTCAGTTG 
GGAATTCAAA CGGTGAAGAG AGTTATTGTT CACGAGAAAT ATAATGGAGC CACCTTCCAA 
AATGACATAG CTTTGATTGA AATGAAAATG CACACGGGCA AGAAAGAATG TGAACTCCCC 
AATTCCGTCC CTGCCTGCGT CCCGTGGTCT CCATATCTGT TCCAACCGAA TGACAGATGT 
ATCATCTCTG GATGGGGTCG AGGGAAAGAT AACCAAAAAG TCTACTCACT CAGGTGGGGC 
GAAGTTGATC TAATAGGCAA CTGCTCTCAG TTTTACCCAG ATCGCTACTA TGAGAAAGAG 
ATGCAGTGCG CAGGTACGCG TGATGGGTCC ATTGATGCCT GCAAAGGAGA CTCCGGAGGC 
CCCTTGGTCT GTGAGGATAT CAACAATGTC ACTTACGTTT GGGGCATTGT GAGCTGGGGA 
GAAAATTGTG GGAAGCCCGA GTTCCCAGGT GTTTACACCA GAGTGGCCAA TTATTTTGAT 
TGGATTAGCT ATCACGTAGG AAGGTCTCTT GTTTCTCAAC ACAATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cfi ( XM_006500963.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501026.1 Mus musculus complement component factor i (Cfi), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu31596
Clone ID Related Accession (Same CDS sequence)	XM_006500963.3 , XM_006500963.2
Accession Version	XM_006500963.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	complement factor I isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500963.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAC 
CGACAGCAGA ACATTCCAAA AGGAGAAGCT GAAATTGAAA CTGAAGAAAC AGAAATGTTG 
ACTCCTGGTA TGGACAATGA AAGAAAACGG ATAAAGTCCT TATTACCTAA ACTCTCCTGT 
GGAGTCAAAA GAAACACTCA CACTCGCAGG AAACGAGTGA TCGGAGGGAA GCCAGCGAAT 
GTGGGAGACT ACCCATGGCA GGTGGCAATT AAGGATGGCC AAAGAATCAC CTGTGGTGGC 
ATTTATATCG GTGGCTGTTG GATTCTGACT GCTGCGCACT GTGTCAGACC CAGTAGAGCT 
CACAGTTACC AAGTCTGGAC GGCTTTATTA GACTGGCTAA AACCTAACTC TCAGTTGGGA 
ATTCAAACGG TGAAGAGAGT TATTGTTCAC GAGAAATATA ATGGAGCCAC CTTCCAAAAT 
GACATAGCTT TGATTGAAAT GAAAATGCAC ACGGGCAAGA AAGAATGTGA ACTCCCCAAT 
TCCGTCCCTG CCTGCGTCCC GTGGTCTCCA TATCTGTTCC AACCGAATGA CAGATGTATC 
ATCTCTGGAT GGGGTCGAGG GAAAGATAAC CAAAAAGTCT ACTCACTCAG GTGGGGCGAA 
GTTGATCTAA TAGGCAACTG CTCTCAGTTT TACCCAGATC GCTACTATGA GAAAGAGATG 
CAGTGCGCAG GTACGCGTGA TGGGTCCATT GATGCCTGCA AAGGAGACTC CGGAGGCCCC 
TTGGTCTGTG AGGATATCAA CAATGTCACT TACGTTTGGG GCATTGTGAG CTGGGGAGAA 
AATTGTGGGA AGCCCGAGTT CCCAGGTGTT TACACCAGAG TGGCCAATTA TTTTGATTGG 
ATTAGCTATC ACGTAGGAAG GTCTCTTGTT TCTCAACACA ATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501026.1
CDS	84..1871
Translation	MKLAHLSLFLLALHLSSSRSPSASDLPQEELVDQKCLLQKYTHRSCNKVFCQPWQRCIEGTCICKLPYQCPRAGTPVCAMNGRSYPTYCHQKSFECLHPEIKFSHNGTCAAEGKFNVSLIYGRTKTEGLVQVKLVDQDERMFICKNSWSMAEANVACVDLGFPLGVRDIQGSFNISGNLHINDTECLHVHCRGVETSLAECAFTKRRTELSNGLAGVVCYKQDADFPTSLSFQCVNGKHIPQEKACNGVNDCGDQSDELCCKGCRGNASLCKSGVCIPDQYKCNGEVDCITGEDESRCEDRQQNIPKGEAEIETEETEMLTPGMDNERKRIKSLLPKLSCGVKRNTHTRRKRVIGGKPANVGDYPWQVAIKDGQRITCGGIYIGGCWILTAAHCVRPSRAHSYQVWTALLDWLKPNSQLGIQTVKRVIVHEKYNGATFQNDIALIEMKMHTGKKECELPNSVPACVPWSPYLFQPNDRCIISGWGRGKDNQKVYSLRWGEVDLIGNCSQFYPDRYYEKEMQCAGTRDGSIDACKGDSGGPLVCEDINNVTYVWGIVSWGENCGKPEFPGVYTRVANYFDWISYHVGRSLVSQHNV

Target ORF information:

RefSeq Version	XM_006500963.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component factor i (Cfi), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500963.2

ORF Insert Sequence:

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAC 
CGACAGCAGA ACATTCCAAA AGGAGAAGCT GAAATTGAAA CTGAAGAAAC AGAAATGTTG 
ACTCCTGGTA TGGACAATGA AAGAAAACGG ATAAAGTCCT TATTACCTAA ACTCTCCTGT 
GGAGTCAAAA GAAACACTCA CACTCGCAGG AAACGAGTGA TCGGAGGGAA GCCAGCGAAT 
GTGGGAGACT ACCCATGGCA GGTGGCAATT AAGGATGGCC AAAGAATCAC CTGTGGTGGC 
ATTTATATCG GTGGCTGTTG GATTCTGACT GCTGCGCACT GTGTCAGACC CAGTAGAGCT 
CACAGTTACC AAGTCTGGAC GGCTTTATTA GACTGGCTAA AACCTAACTC TCAGTTGGGA 
ATTCAAACGG TGAAGAGAGT TATTGTTCAC GAGAAATATA ATGGAGCCAC CTTCCAAAAT 
GACATAGCTT TGATTGAAAT GAAAATGCAC ACGGGCAAGA AAGAATGTGA ACTCCCCAAT 
TCCGTCCCTG CCTGCGTCCC GTGGTCTCCA TATCTGTTCC AACCGAATGA CAGATGTATC 
ATCTCTGGAT GGGGTCGAGG GAAAGATAAC CAAAAAGTCT ACTCACTCAG GTGGGGCGAA 
GTTGATCTAA TAGGCAACTG CTCTCAGTTT TACCCAGATC GCTACTATGA GAAAGAGATG 
CAGTGCGCAG GTACGCGTGA TGGGTCCATT GATGCCTGCA AAGGAGACTC CGGAGGCCCC 
TTGGTCTGTG AGGATATCAA CAATGTCACT TACGTTTGGG GCATTGTGAG CTGGGGAGAA 
AATTGTGGGA AGCCCGAGTT CCCAGGTGTT TACACCAGAG TGGCCAATTA TTTTGATTGG 
ATTAGCTATC ACGTAGGAAG GTCTCTTGTT TCTCAACACA ATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cfi ( XM_006500962.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501025.1 Mus musculus complement component factor i (Cfi), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31595
Clone ID Related Accession (Same CDS sequence)	XM_006500962.2 , XM_006500962.3
Accession Version	XM_006500962.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	complement factor I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500962.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAA 
GACCGACAGC AGAACATTCC AAAAGGAGAA GCTGAAATTG AAACTGAAGA AACAGAAATG 
TTGACTCCTG GTATGGACAA TGAAAGAAAA CGGATAAAGT CCTTATTACC TAAACTCTCC 
TGTGGAGTCA AAAGAAACAC TCACACTCGC AGGAAACGAG TGATCGGAGG GAAGCCAGCG 
AATGTGGGAG ACTACCCATG GCAGGTGGCA ATTAAGGATG GCCAAAGAAT CACCTGTGGT 
GGCATTTATA TCGGTGGCTG TTGGATTCTG ACTGCTGCGC ACTGTGTCAG ACCCAGTAGA 
GCTCACAGTT ACCAAGTCTG GACGGCTTTA TTAGACTGGC TAAAACCTAA CTCTCAGTTG 
GGAATTCAAA CGGTGAAGAG AGTTATTGTT CACGAGAAAT ATAATGGAGC CACCTTCCAA 
AATGACATAG CTTTGATTGA AATGAAAATG CACACGGGCA AGAAAGAATG TGAACTCCCC 
AATTCCGTCC CTGCCTGCGT CCCGTGGTCT CCATATCTGT TCCAACCGAA TGACAGATGT 
ATCATCTCTG GATGGGGTCG AGGGAAAGAT AACCAAAAAG TCTACTCACT CAGGTGGGGC 
GAAGTTGATC TAATAGGCAA CTGCTCTCAG TTTTACCCAG ATCGCTACTA TGAGAAAGAG 
ATGCAGTGCG CAGGTACGCG TGATGGGTCC ATTGATGCCT GCAAAGGAGA CTCCGGAGGC 
CCCTTGGTCT GTGAGGATAT CAACAATGTC ACTTACGTTT GGGGCATTGT GAGCTGGGGA 
GAAAATTGTG GGAAGCCCGA GTTCCCAGGT GTTTACACCA GAGTGGCCAA TTATTTTGAT 
TGGATTAGCT ATCACGTAGG AAGGTCTCTT GTTTCTCAAC ACAATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501025.1
CDS	83..1873
Translation	MKLAHLSLFLLALHLSSSRSPSASDLPQEELVDQKCLLQKYTHRSCNKVFCQPWQRCIEGTCICKLPYQCPRAGTPVCAMNGRSYPTYCHQKSFECLHPEIKFSHNGTCAAEGKFNVSLIYGRTKTEGLVQVKLVDQDERMFICKNSWSMAEANVACVDLGFPLGVRDIQGSFNISGNLHINDTECLHVHCRGVETSLAECAFTKRRTELSNGLAGVVCYKQDADFPTSLSFQCVNGKHIPQEKACNGVNDCGDQSDELCCKGCRGNASLCKSGVCIPDQYKCNGEVDCITGEDESRCEEDRQQNIPKGEAEIETEETEMLTPGMDNERKRIKSLLPKLSCGVKRNTHTRRKRVIGGKPANVGDYPWQVAIKDGQRITCGGIYIGGCWILTAAHCVRPSRAHSYQVWTALLDWLKPNSQLGIQTVKRVIVHEKYNGATFQNDIALIEMKMHTGKKECELPNSVPACVPWSPYLFQPNDRCIISGWGRGKDNQKVYSLRWGEVDLIGNCSQFYPDRYYEKEMQCAGTRDGSIDACKGDSGGPLVCEDINNVTYVWGIVSWGENCGKPEFPGVYTRVANYFDWISYHVGRSLVSQHNV

Target ORF information:

RefSeq Version	XM_006500962.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component factor i (Cfi), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500962.3

ORF Insert Sequence:

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAA 
GACCGACAGC AGAACATTCC AAAAGGAGAA GCTGAAATTG AAACTGAAGA AACAGAAATG 
TTGACTCCTG GTATGGACAA TGAAAGAAAA CGGATAAAGT CCTTATTACC TAAACTCTCC 
TGTGGAGTCA AAAGAAACAC TCACACTCGC AGGAAACGAG TGATCGGAGG GAAGCCAGCG 
AATGTGGGAG ACTACCCATG GCAGGTGGCA ATTAAGGATG GCCAAAGAAT CACCTGTGGT 
GGCATTTATA TCGGTGGCTG TTGGATTCTG ACTGCTGCGC ACTGTGTCAG ACCCAGTAGA 
GCTCACAGTT ACCAAGTCTG GACGGCTTTA TTAGACTGGC TAAAACCTAA CTCTCAGTTG 
GGAATTCAAA CGGTGAAGAG AGTTATTGTT CACGAGAAAT ATAATGGAGC CACCTTCCAA 
AATGACATAG CTTTGATTGA AATGAAAATG CACACGGGCA AGAAAGAATG TGAACTCCCC 
AATTCCGTCC CTGCCTGCGT CCCGTGGTCT CCATATCTGT TCCAACCGAA TGACAGATGT 
ATCATCTCTG GATGGGGTCG AGGGAAAGAT AACCAAAAAG TCTACTCACT CAGGTGGGGC 
GAAGTTGATC TAATAGGCAA CTGCTCTCAG TTTTACCCAG ATCGCTACTA TGAGAAAGAG 
ATGCAGTGCG CAGGTACGCG TGATGGGTCC ATTGATGCCT GCAAAGGAGA CTCCGGAGGC 
CCCTTGGTCT GTGAGGATAT CAACAATGTC ACTTACGTTT GGGGCATTGT GAGCTGGGGA 
GAAAATTGTG GGAAGCCCGA GTTCCCAGGT GTTTACACCA GAGTGGCCAA TTATTTTGAT 
TGGATTAGCT ATCACGTAGG AAGGTCTCTT GTTTCTCAAC ACAATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cfi ( XM_006500963.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501026.1 Mus musculus complement component factor i (Cfi), transcript variant X2, mRNA.

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CloneID	OMu31596
Clone ID Related Accession (Same CDS sequence)	XM_006500963.3 , XM_006500963.2
Accession Version	XM_006500963.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	complement factor I isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500963.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAC 
CGACAGCAGA ACATTCCAAA AGGAGAAGCT GAAATTGAAA CTGAAGAAAC AGAAATGTTG 
ACTCCTGGTA TGGACAATGA AAGAAAACGG ATAAAGTCCT TATTACCTAA ACTCTCCTGT 
GGAGTCAAAA GAAACACTCA CACTCGCAGG AAACGAGTGA TCGGAGGGAA GCCAGCGAAT 
GTGGGAGACT ACCCATGGCA GGTGGCAATT AAGGATGGCC AAAGAATCAC CTGTGGTGGC 
ATTTATATCG GTGGCTGTTG GATTCTGACT GCTGCGCACT GTGTCAGACC CAGTAGAGCT 
CACAGTTACC AAGTCTGGAC GGCTTTATTA GACTGGCTAA AACCTAACTC TCAGTTGGGA 
ATTCAAACGG TGAAGAGAGT TATTGTTCAC GAGAAATATA ATGGAGCCAC CTTCCAAAAT 
GACATAGCTT TGATTGAAAT GAAAATGCAC ACGGGCAAGA AAGAATGTGA ACTCCCCAAT 
TCCGTCCCTG CCTGCGTCCC GTGGTCTCCA TATCTGTTCC AACCGAATGA CAGATGTATC 
ATCTCTGGAT GGGGTCGAGG GAAAGATAAC CAAAAAGTCT ACTCACTCAG GTGGGGCGAA 
GTTGATCTAA TAGGCAACTG CTCTCAGTTT TACCCAGATC GCTACTATGA GAAAGAGATG 
CAGTGCGCAG GTACGCGTGA TGGGTCCATT GATGCCTGCA AAGGAGACTC CGGAGGCCCC 
TTGGTCTGTG AGGATATCAA CAATGTCACT TACGTTTGGG GCATTGTGAG CTGGGGAGAA 
AATTGTGGGA AGCCCGAGTT CCCAGGTGTT TACACCAGAG TGGCCAATTA TTTTGATTGG 
ATTAGCTATC ACGTAGGAAG GTCTCTTGTT TCTCAACACA ATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501026.1
CDS	84..1871
Translation	MKLAHLSLFLLALHLSSSRSPSASDLPQEELVDQKCLLQKYTHRSCNKVFCQPWQRCIEGTCICKLPYQCPRAGTPVCAMNGRSYPTYCHQKSFECLHPEIKFSHNGTCAAEGKFNVSLIYGRTKTEGLVQVKLVDQDERMFICKNSWSMAEANVACVDLGFPLGVRDIQGSFNISGNLHINDTECLHVHCRGVETSLAECAFTKRRTELSNGLAGVVCYKQDADFPTSLSFQCVNGKHIPQEKACNGVNDCGDQSDELCCKGCRGNASLCKSGVCIPDQYKCNGEVDCITGEDESRCEDRQQNIPKGEAEIETEETEMLTPGMDNERKRIKSLLPKLSCGVKRNTHTRRKRVIGGKPANVGDYPWQVAIKDGQRITCGGIYIGGCWILTAAHCVRPSRAHSYQVWTALLDWLKPNSQLGIQTVKRVIVHEKYNGATFQNDIALIEMKMHTGKKECELPNSVPACVPWSPYLFQPNDRCIISGWGRGKDNQKVYSLRWGEVDLIGNCSQFYPDRYYEKEMQCAGTRDGSIDACKGDSGGPLVCEDINNVTYVWGIVSWGENCGKPEFPGVYTRVANYFDWISYHVGRSLVSQHNV

Target ORF information:

RefSeq Version	XM_006500963.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component factor i (Cfi), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500963.3

ORF Insert Sequence:

ATGAAGCTCG CTCATCTCAG TCTCTTTCTC TTGGCTCTCC ACTTGAGTTC TTCCAGGTCC 
CCGTCAGCAT CAGATCTGCC TCAGGAGGAG CTGGTCGATC AGAAATGCTT ACTGCAGAAA 
TACACGCATC GCTCCTGTAA CAAGGTCTTC TGCCAGCCGT GGCAGAGATG TATCGAAGGC 
ACCTGTATCT GCAAACTCCC CTACCAGTGC CCAAGGGCTG GCACCCCAGT GTGTGCCATG 
AATGGAAGGA GCTACCCGAC ATACTGTCAC CAGAAGAGTT TCGAATGTCT TCACCCAGAG 
ATCAAGTTTT CACATAATGG AACGTGCGCA GCTGAAGGAA AGTTTAATGT TTCCTTAATT 
TATGGGAGAA CAAAAACAGA GGGACTTGTT CAAGTCAAAC TTGTGGACCA AGATGAGAGA 
ATGTTCATAT GTAAAAACAG CTGGAGCATG GCGGAGGCCA ACGTGGCCTG CGTCGACCTT 
GGATTTCCAC TGGGTGTTCG TGACATACAA GGAAGTTTTA ATATATCTGG AAATCTCCAT 
ATAAACGACA CTGAATGCCT GCATGTACAT TGCCGGGGAG TAGAGACCAG TTTGGCGGAG 
TGTGCCTTTA CGAAGAGGAG AACTGAGTTG TCCAATGGCT TGGCGGGGGT AGTGTGTTAC 
AAGCAGGATG CAGATTTCCC AACGAGTCTG TCCTTCCAGT GTGTGAATGG GAAGCACATT 
CCTCAGGAGA AAGCCTGCAA CGGTGTCAAT GACTGTGGAG ACCAAAGCGA TGAGCTGTGT 
TGCAAAGGTT GCCGAGGTAA CGCTTCCCTT TGTAAGTCGG GAGTTTGCAT TCCAGACCAA 
TACAAGTGTA ATGGTGAGGT GGACTGCATC ACTGGTGAAG ATGAGAGCCG TTGTGAAGAC 
CGACAGCAGA ACATTCCAAA AGGAGAAGCT GAAATTGAAA CTGAAGAAAC AGAAATGTTG 
ACTCCTGGTA TGGACAATGA AAGAAAACGG ATAAAGTCCT TATTACCTAA ACTCTCCTGT 
GGAGTCAAAA GAAACACTCA CACTCGCAGG AAACGAGTGA TCGGAGGGAA GCCAGCGAAT 
GTGGGAGACT ACCCATGGCA GGTGGCAATT AAGGATGGCC AAAGAATCAC CTGTGGTGGC 
ATTTATATCG GTGGCTGTTG GATTCTGACT GCTGCGCACT GTGTCAGACC CAGTAGAGCT 
CACAGTTACC AAGTCTGGAC GGCTTTATTA GACTGGCTAA AACCTAACTC TCAGTTGGGA 
ATTCAAACGG TGAAGAGAGT TATTGTTCAC GAGAAATATA ATGGAGCCAC CTTCCAAAAT 
GACATAGCTT TGATTGAAAT GAAAATGCAC ACGGGCAAGA AAGAATGTGA ACTCCCCAAT 
TCCGTCCCTG CCTGCGTCCC GTGGTCTCCA TATCTGTTCC AACCGAATGA CAGATGTATC 
ATCTCTGGAT GGGGTCGAGG GAAAGATAAC CAAAAAGTCT ACTCACTCAG GTGGGGCGAA 
GTTGATCTAA TAGGCAACTG CTCTCAGTTT TACCCAGATC GCTACTATGA GAAAGAGATG 
CAGTGCGCAG GTACGCGTGA TGGGTCCATT GATGCCTGCA AAGGAGACTC CGGAGGCCCC 
TTGGTCTGTG AGGATATCAA CAATGTCACT TACGTTTGGG GCATTGTGAG CTGGGGAGAA 
AATTGTGGGA AGCCCGAGTT CCCAGGTGTT TACACCAGAG TGGCCAATTA TTTTGATTGG 
ATTAGCTATC ACGTAGGAAG GTCTCTTGTT TCTCAACACA ATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

C3 opsonization regulates endocytic handling of apoptotic cells resulting in enhanced T-cell responses to cargo-derived antigens.
Proceedings of the National Academy of Sciences of the United States of America111(4)1503-8(2014 Jan)
Baudino L,Sardini A,Ruseva MM,Fossati-Jimack L,Cook HT,Scott D,Simpson E,Botto M

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)
Lumayag S,Haldin CE,Corbett NJ,Wahlin KJ,Cowan C,Turturro S,Larsen PE,Kovacs B,Witmer PD,Valle D,Zack DJ,Nicholson DA,Xu S

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Cfi cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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