Ctla4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ctla4
Entrez Gene ID	12477
Full Name	cytotoxic T-lymphocyte-associated protein 4
Synonyms	Cd152,Ctla-4,Ly-56
General protein information	Preferred Names cytotoxic T-lymphocyte-associated protein 4 Names cytotoxic T-lymphocyte protein 4 CD152 antigen cytotoxic T-lymphocyte-associated antigen 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C2\|1 30.58 cM
Summary	This gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009843.4	NP_033973.2	cytotoxic T-lymphocyte protein 4 isoform 1 precursor
NM_001281976.1	NP_001268905.1	cytotoxic T-lymphocyte protein 4 isoform 2 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ctla4	NP_113862.1
Pan troglodytes (chimpanzee)	CTLA4	XP_526000.1
Mus musculus (house mouse)	Ctla4	NP_033973.2
Xenopus tropicalis (tropical clawed frog)	ctla4	XP_002936576.1
Bos taurus (cattle)	CTLA4	NP_776722.1
Homo sapiens (human)	CTLA4	NP_005205.2
	CTLA4	NP_001038204.1
Canis lupus familiaris (dog)	CTLA4	NP_001003106.1
Gallus gallus (chicken)	CTLA4	NP_001035180.1

Gene Ontology
Bibliography

Related articles in PubMed

The soluble isoform of CTLA-4 as a regulator of T-cell responses.
Ward FJ, Dahal LN, Wijesekera SK, Abdul-Jawad SK, Kaewarpai T, Xu H, Vickers MA, Barker RN
European journal of immunology43(5)1274-85(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tregs were observed to regulate CD4(+), but not CD8(+), T cell infiltration into tumors through a CTLA-4/CD80 dependent mechanism. Disrupting CTLA-4 interaction with CD80 was sufficient to induce CD4 T cell infiltration into tumors.
Title: CTLA-4/CD80 pathway regulates T cell infiltration into pancreatic cancer.

Treg cells expand in both humans and mice in blood-stage malaria and interfere with conventional T helper cell responses and follicular T helper (TFH)-B cell interactions in germinal centers. Mechanistically, Treg cells function in a critical temporal window to impede protective immunity through cytotoxic-T-lymphocyte-associated protein-4 (CTLA-4).
Title: Regulatory T cells impede acute and long-term immunity to blood-stage malaria through CTLA-4.

CTLA-4 expressed by FOXP3(+) regulatory T cells prevents inflammatory tissue attack and not T-cell priming in arthritis.
Title: CTLA-4 expressed by FOXP3

These results suggest that CD44(+)CD117(+) T cells are stem cells and a specific T-cell phenotype that initially develops in the thymus, but they do not progress through DN3 and DN4 stages, lack a DP stage, and potently suppress T-cell proliferation and modulate the CTLA-4 pathway.
Title: CD117

data suggest that increased expression of checkpoint blockade molecules PD-1 and CTLA-4 on donor T cells is not sufficient to prevent GvHD, and that cooperation between checkpoint blockade signaling by host cells and donor Tregs is necessary to limit GvHD in allo-HSCT recipients
Title: PD-1 and CTLA-4 up regulation on donor T cells is insufficient to prevent GvHD in allo-HSCT recipients.

The following Ctla4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ctla4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18250	NM_001281976.1 Latest version!	Mus musculus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu18313	NM_009843.4 Latest version!	Mus musculus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ctla4 ( NM_001281976.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001268905.1 Mus musculus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18250
Clone ID Related Accession (Same CDS sequence)	NM_001281976.1
Accession Version	NM_001281976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	cytotoxic T-lymphocyte protein 4 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL663047.15, AK138891.1 and AI323635.1. Transcript Variant: This variant (2) lacks the penultimate coding exon, which results in a frame-shift and early translation termination compared to variant 1. The resulting shorter isoform (2, also known as sCTLA-4) with a distinct C-terminus, lacks the transmembrane domain and is secreted (PMIDs: 10831323, 23400950). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849375, SAMN00849377 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC TTGGACTCCG GAGGTACAAA GCTCAACTGC AGCTGCCTTC TAGGACTTGG 
CCTTTTGTAG CCCTGCTCAC TCTTCTTTTC ATCCCAGTCT TCTCTGAAGC CATACAGGTG 
ACCCAACCTT CAGTGGTGTT GGCTAGCAGC CATGGTGTCG CCAGCTTTCC ATGTGAATAT 
TCACCATCAC ACAACACTGA TGAGGTCCGG GTGACTGTGC TGCGGCAGAC AAATGACCAA 
ATGACTGAGG TCTGTGCCAC GACATTCACA GAGAAGAATA CAGTGGGCTT CCTAGATTAC 
CCCTTCTGCA GTGGTACCTT TAATGAAAGC AGAGTGAACC TCACCATCCA AGGACTGAGA 
GCTGTTGACA CGGGACTGTA CCTCTGCAAG GTGGAACTCA TGTACCCACC GCCATACTTT 
GTGGGCATGG GCAACGGGAC GCAGATTTAT GTCATTGCTA AAGAAAAGAA GTCCTCTTAC 
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268905.1
CDS	147..671
Translation	MACLGLRRYKAQLQLPSRTWPFVALLTLLFIPVFSEAIQVTQPSVVLASSHGVASFPCEYSPSHNTDEVRVTVLRQTNDQMTEVCATTFTEKNTVGFLDYPFCSGTFNESRVNLTIQGLRAVDTGLYLCKVELMYPPPYFVGMGNGTQIYVIAKEKKSSYNRGLCENAPNRARM

Target ORF information:

RefSeq Version	NM_001281976.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281976.1

ORF Insert Sequence:

ATGGCTTGTC TTGGACTCCG GAGGTACAAA GCTCAACTGC AGCTGCCTTC TAGGACTTGG 
CCTTTTGTAG CCCTGCTCAC TCTTCTTTTC ATCCCAGTCT TCTCTGAAGC CATACAGGTG 
ACCCAACCTT CAGTGGTGTT GGCTAGCAGC CATGGTGTCG CCAGCTTTCC ATGTGAATAT 
TCACCATCAC ACAACACTGA TGAGGTCCGG GTGACTGTGC TGCGGCAGAC AAATGACCAA 
ATGACTGAGG TCTGTGCCAC GACATTCACA GAGAAGAATA CAGTGGGCTT CCTAGATTAC 
CCCTTCTGCA GTGGTACCTT TAATGAAAGC AGAGTGAACC TCACCATCCA AGGACTGAGA 
GCTGTTGACA CGGGACTGTA CCTCTGCAAG GTGGAACTCA TGTACCCACC GCCATACTTT 
GTGGGCATGG GCAACGGGAC GCAGATTTAT GTCATTGCTA AAGAAAAGAA GTCCTCTTAC 
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctla4 ( NM_009843.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033973.2 Mus musculus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18313
Clone ID Related Accession (Same CDS sequence)	NM_009843.4
Accession Version	NM_009843.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	cytotoxic T-lymphocyte protein 4 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL663047.15, AK138891.1 and AI323635.1. On Aug 13, 2013 this sequence version replaced NM_009843.3. Transcript Variant: This variant (1) encodes the longer membrane-bound isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC042741.1, BC052683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGTC TTGGACTCCG GAGGTACAAA GCTCAACTGC AGCTGCCTTC TAGGACTTGG 
CCTTTTGTAG CCCTGCTCAC TCTTCTTTTC ATCCCAGTCT TCTCTGAAGC CATACAGGTG 
ACCCAACCTT CAGTGGTGTT GGCTAGCAGC CATGGTGTCG CCAGCTTTCC ATGTGAATAT 
TCACCATCAC ACAACACTGA TGAGGTCCGG GTGACTGTGC TGCGGCAGAC AAATGACCAA 
ATGACTGAGG TCTGTGCCAC GACATTCACA GAGAAGAATA CAGTGGGCTT CCTAGATTAC 
CCCTTCTGCA GTGGTACCTT TAATGAAAGC AGAGTGAACC TCACCATCCA AGGACTGAGA 
GCTGTTGACA CGGGACTGTA CCTCTGCAAG GTGGAACTCA TGTACCCACC GCCATACTTT 
GTGGGCATGG GCAACGGGAC GCAGATTTAT GTCATTGATC CAGAACCATG CCCGGATTCT 
GACTTCCTCC TTTGGATCCT TGTCGCAGTT AGCTTGGGGT TGTTTTTTTA CAGTTTCCTG 
GTCACTGCTG TTTCTTTGAG CAAGATGCTA AAGAAAAGAA GTCCTCTTAC AACAGGGGTC 
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAGCC TTATTTTATT 
CCCATCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033973.2
CDS	147..818
Translation	MACLGLRRYKAQLQLPSRTWPFVALLTLLFIPVFSEAIQVTQPSVVLASSHGVASFPCEYSPSHNTDEVRVTVLRQTNDQMTEVCATTFTEKNTVGFLDYPFCSGTFNESRVNLTIQGLRAVDTGLYLCKVELMYPPPYFVGMGNGTQIYVIDPEPCPDSDFLLWILVAVSLGLFFYSFLVTAVSLSKMLKKRSPLTTGVYVKMPPTEPECEKQFQPYFIPIN

Target ORF information:

RefSeq Version	NM_009843.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009843.4

ORF Insert Sequence:

ATGGCTTGTC TTGGACTCCG GAGGTACAAA GCTCAACTGC AGCTGCCTTC TAGGACTTGG 
CCTTTTGTAG CCCTGCTCAC TCTTCTTTTC ATCCCAGTCT TCTCTGAAGC CATACAGGTG 
ACCCAACCTT CAGTGGTGTT GGCTAGCAGC CATGGTGTCG CCAGCTTTCC ATGTGAATAT 
TCACCATCAC ACAACACTGA TGAGGTCCGG GTGACTGTGC TGCGGCAGAC AAATGACCAA 
ATGACTGAGG TCTGTGCCAC GACATTCACA GAGAAGAATA CAGTGGGCTT CCTAGATTAC 
CCCTTCTGCA GTGGTACCTT TAATGAAAGC AGAGTGAACC TCACCATCCA AGGACTGAGA 
GCTGTTGACA CGGGACTGTA CCTCTGCAAG GTGGAACTCA TGTACCCACC GCCATACTTT 
GTGGGCATGG GCAACGGGAC GCAGATTTAT GTCATTGATC CAGAACCATG CCCGGATTCT 
GACTTCCTCC TTTGGATCCT TGTCGCAGTT AGCTTGGGGT TGTTTTTTTA CAGTTTCCTG 
GTCACTGCTG TTTCTTTGAG CAAGATGCTA AAGAAAAGAA GTCCTCTTAC AACAGGGGTC 
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAGCC TTATTTTATT 
CCCATCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The soluble isoform of CTLA-4 as a regulator of T-cell responses.
European journal of immunology43(5)1274-85(2013 May)
Ward FJ,Dahal LN,Wijesekera SK,Abdul-Jawad SK,Kaewarpai T,Xu H,Vickers MA,Barker RN

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Ctla4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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