Car2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Car2
Entrez Gene ID	12349
Full Name	carbonic anhydrase 2
Synonyms	AI131712,CAII,Ca2,Car-2,Ltw-5,Lvtw-5
General protein information	Preferred Names carbonic anhydrase 2 Names carbonic anhydrase 2 carbonate dehydratase II carbonic anhydrase II
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 A1\|3 3.23 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009801.4	NP_033931.4	carbonic anhydrase 2
XM_006530050.1	XP_006530113.1	carbonic anhydrase 2 isoform X1
NM_001357334.1	NP_001344263.1	carbonic anhydrase 2
NM_009801.5	NP_033931.4	carbonic anhydrase 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Car2	NP_062164.1
	CA2	XP_001096360.1
Pan troglodytes (chimpanzee)	CA2	NP_001181853.1
Bos taurus (cattle)	CA2	NP_848667.1
Gallus gallus (chicken)	CA2	NP_990648.1
Mus musculus (house mouse)	Car2	NP_033931.4
Homo sapiens (human)	CA2	NP_000058.1
Canis lupus familiaris (dog)	CA2	NP_001138642.1
Xenopus tropicalis (tropical clawed frog)	ca2	NP_001015729.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function:
Chatel B, Bendahan D, Hourd? C, Pellerin L, Lengacher S, Magistretti P, Le Fur Y, Vilmen C, Bernard M, Messonnier LA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(6)2562-2575(2017 06)

Transcription factor
Werth M, Schmidt-Ott KM, Leete T, Qiu A, Hinze C, Viltard M, Paragas N, Shawber CJ, Yu W, Lee P, Chen X, Sarkar A, Mu W, Rittenberg A, Lin CS, Kitajewski J, Al-Awqati Q, Barasch J
eLife6(2017 Jun)

Elf5 is a principal cell lineage specific transcription factor in the kidney that contributes to Aqp2 and Avpr2 gene expression.
Grassmeyer J, Mukherjee M, deRiso J, Hettinger C, Bailey M, Sinha S, Visvader JE, Zhao H, Fogarty E, Surendran K
Developmental biology424(1)77-89(2017 04)

Deficiency of Carbonic Anhydrase II Results in a Urinary Concentrating Defect.
Krishnan D, Pan W, Beggs MR, Trepiccione F, Chambrey R, Eladari D, Cordat E, Dimke H, Alexander RT
Frontiers in physiology81108(2017)

Basal cells express functional TRPV4 channels in the mouse nasal epithelium.
Ueda T, Hoshikawa M, Shibata Y, Kumamoto N, Ugawa S
Biochemistry and biophysics reports4169-174(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It was concluded that both MCT1 and CAII are involved in the homeostatic control of pH in skeletal muscle, both at rest and at the onset of exercise. The improved muscle function and resistance to fatigue in MCT1(+/-) mice remain unexplained.
Title: Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function:

two CA isoforms, CAII and CAIV, are distributed along the epididymal epithelium and appear with the onset of puberty
Title: Normal Fertility Requires the Expression of Carbonic Anhydrases II and IV in Sperm.

CA2 expression is strongly regulated by intracellular pH and by cAMP, suggesting a role for soluble adenylyl cyclase
Title: Cyclic AMP and alkaline pH downregulate carbonic anhydrase 2 in mouse fibroblasts.

sclerostin upregulated osteocyte expression of carbonic anhydrase 2.
Title: Sclerostin regulates release of bone mineral by osteocytes by induction of carbonic anhydrase 2.

The Na(+)-Cl(-) cotransporter NCC /Carbonic anhydrase II double Knockout mice displayed significant downregulation of pendrin, along with polyuria and salt wasting.
Title: Double knockout of carbonic anhydrase II (CAII) and Na(+)-Cl(-) cotransporter (NCC) causes salt wasting and volume depletion.

The following Car2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Car2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13121	NM_009801.5 Latest version!	Mus musculus carbonic anhydrase 2 (Car2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu13121	NM_001357334.1 Latest version!	Mus musculus carbonic anhydrase 2 (Car2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu13121	XM_006530050.1 Latest version!	Mus musculus carbonic anhydrase 2 (Car2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OMu13121	NM_009801.4	Mus musculus carbonic anhydrase 2 (Car2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Car2 ( NM_009801.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033931.4 Mus musculus carbonic anhydrase 2 (Car2), mRNA.

Price & Availability↑

CloneID	OMu13121
Clone ID Related Accession (Same CDS sequence)	NM_009801.4 , XM_006530050.1 , NM_001357334.1 , NM_009801.5
Accession Version	NM_009801.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-29
Organism	Mus musculus(House mouse)
Product	carbonic anhydrase 2
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU946040.1, AK002333.1 and CD774528.1. On Oct 6, 2007 this sequence version replaced NM_009801.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC055291.1, SRR1660815.11432.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCACC ACTGGGGATA CAGCAAGCAC AACGGACCAG AGAACTGGCA CAAGGACTTC 
CCCATTGCCA ATGGAGACCG GCAGTCCCCT GTGGACATTG ACACAGCAAC TGCCCAGCAT 
GACCCTGCCC TACAGCCTCT GCTCATATCT TATGATAAAG CTGCGTCCAA GAGCATTGTC 
AACAACGGCC ACTCCTTTAA CGTTGAGTTT GATGACTCTC AGGACAATGC AGTGCTGAAA 
GGAGGACCCC TCAGTGACTC CTACAGATTG ATCCAGTTTC ACTTTCACTG GGGCTCATCT 
GATGGCCAGG GCTCTGAGCA CACTGTGAAC AAAAAAAAAT ATGCTGCAGA GCTTCACTTG 
GTTCACTGGA ACACCAAATA TGGGGACTTT GGAAAAGCTG TGCAGCAACC GGATGGATTG 
GCTGTTTTGG GTATTTTTTT GAAGATTGGA CCTGCCTCAC AAGGCCTTCA GAAAGTCCTT 
GAAGCACTGC ATTCCATTAA AACAAAGGGG AAGCGTGCGG CCTTTGCTAA CTTCGATCCT 
TGCTCCCTTC TTCCTGGAAA CTTGGACTAC TGGACATACC CTGGCTCTCT GACCACTCCG 
CCTCTGCTGG AATGTGTGAC CTGGATCGTG CTCAGGGAGC CCATTACTGT CAGCAGCGAG 
CAGATGTCTC ATTTCCGTAC GCTGAACTTC AATGAGGAGG GGGATGCTGA AGAAGCGATG 
GTGGACAACT GGCGTCCAGC TCAGCCGCTA AAGAATAGAA AGATCAAAGC GTCCTTTAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033931.4
CDS	328..1110
Translation	MSHHWGYSKHNGPENWHKDFPIANGDRQSPVDIDTATAQHDPALQPLLISYDKAASKSIVNNGHSFNVEFDDSQDNAVLKGGPLSDSYRLIQFHFHWGSSDGQGSEHTVNKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKIGPASQGLQKVLEALHSIKTKGKRAAFANFDPCSLLPGNLDYWTYPGSLTTPPLLECVTWIVLREPITVSSEQMSHFRTLNFNEEGDAEEAMVDNWRPAQPLKNRKIKASFK

Target ORF information:

RefSeq Version	NM_009801.4
Organism	Mus musculus(House mouse)
Definition	Mus musculus carbonic anhydrase 2 (Car2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009801.4

ORF Insert Sequence:

ATGTCCCACC ACTGGGGATA CAGCAAGCAC AACGGACCAG AGAACTGGCA CAAGGACTTC 
CCCATTGCCA ATGGAGACCG GCAGTCCCCT GTGGACATTG ACACAGCAAC TGCCCAGCAT 
GACCCTGCCC TACAGCCTCT GCTCATATCT TATGATAAAG CTGCGTCCAA GAGCATTGTC 
AACAACGGCC ACTCCTTTAA CGTTGAGTTT GATGACTCTC AGGACAATGC AGTGCTGAAA 
GGAGGACCCC TCAGTGACTC CTACAGATTG ATCCAGTTTC ACTTTCACTG GGGCTCATCT 
GATGGCCAGG GCTCTGAGCA CACTGTGAAC AAAAAAAAAT ATGCTGCAGA GCTTCACTTG 
GTTCACTGGA ACACCAAATA TGGGGACTTT GGAAAAGCTG TGCAGCAACC GGATGGATTG 
GCTGTTTTGG GTATTTTTTT GAAGATTGGA CCTGCCTCAC AAGGCCTTCA GAAAGTCCTT 
GAAGCACTGC ATTCCATTAA AACAAAGGGG AAGCGTGCGG CCTTTGCTAA CTTCGATCCT 
TGCTCCCTTC TTCCTGGAAA CTTGGACTAC TGGACATACC CTGGCTCTCT GACCACTCCG 
CCTCTGCTGG AATGTGTGAC CTGGATCGTG CTCAGGGAGC CCATTACTGT CAGCAGCGAG 
CAGATGTCTC ATTTCCGTAC GCTGAACTTC AATGAGGAGG GGGATGCTGA AGAAGCGATG 
GTGGACAACT GGCGTCCAGC TCAGCCGCTA AAGAATAGAA AGATCAAAGC GTCCTTTAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Car2 ( NM_001357334.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344263.1 Mus musculus carbonic anhydrase 2 (Car2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13121
Clone ID Related Accession (Same CDS sequence)	NM_009801.4 , XM_006530050.1 , NM_001357334.1 , NM_009801.5
Accession Version	NM_001357334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	carbonic anhydrase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC123747.26, CK618053.1, AC098725.3 and CD774528.1. On Oct 26, 2017 this sequence version replaced XM_006530050.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.118116.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCACC ACTGGGGATA CAGCAAGCAC AACGGACCAG AGAACTGGCA CAAGGACTTC 
CCCATTGCCA ATGGAGACCG GCAGTCCCCT GTGGACATTG ACACAGCAAC TGCCCAGCAT 
GACCCTGCCC TACAGCCTCT GCTCATATCT TATGATAAAG CTGCGTCCAA GAGCATTGTC 
AACAACGGCC ACTCCTTTAA CGTTGAGTTT GATGACTCTC AGGACAATGC AGTGCTGAAA 
GGAGGACCCC TCAGTGACTC CTACAGATTG ATCCAGTTTC ACTTTCACTG GGGCTCATCT 
GATGGCCAGG GCTCTGAGCA CACTGTGAAC AAAAAAAAAT ATGCTGCAGA GCTTCACTTG 
GTTCACTGGA ACACCAAATA TGGGGACTTT GGAAAAGCTG TGCAGCAACC GGATGGATTG 
GCTGTTTTGG GTATTTTTTT GAAGATTGGA CCTGCCTCAC AAGGCCTTCA GAAAGTCCTT 
GAAGCACTGC ATTCCATTAA AACAAAGGGG AAGCGTGCGG CCTTTGCTAA CTTCGATCCT 
TGCTCCCTTC TTCCTGGAAA CTTGGACTAC TGGACATACC CTGGCTCTCT GACCACTCCG 
CCTCTGCTGG AATGTGTGAC CTGGATCGTG CTCAGGGAGC CCATTACTGT CAGCAGCGAG 
CAGATGTCTC ATTTCCGTAC GCTGAACTTC AATGAGGAGG GGGATGCTGA AGAAGCGATG 
GTGGACAACT GGCGTCCAGC TCAGCCGCTA AAGAATAGAA AGATCAAAGC GTCCTTTAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344263.1
CDS	247..1029
Translation	MSHHWGYSKHNGPENWHKDFPIANGDRQSPVDIDTATAQHDPALQPLLISYDKAASKSIVNNGHSFNVEFDDSQDNAVLKGGPLSDSYRLIQFHFHWGSSDGQGSEHTVNKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKIGPASQGLQKVLEALHSIKTKGKRAAFANFDPCSLLPGNLDYWTYPGSLTTPPLLECVTWIVLREPITVSSEQMSHFRTLNFNEEGDAEEAMVDNWRPAQPLKNRKIKASFK

Target ORF information:

RefSeq Version	NM_001357334.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbonic anhydrase 2 (Car2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357334.1

ORF Insert Sequence:

ATGTCCCACC ACTGGGGATA CAGCAAGCAC AACGGACCAG AGAACTGGCA CAAGGACTTC 
CCCATTGCCA ATGGAGACCG GCAGTCCCCT GTGGACATTG ACACAGCAAC TGCCCAGCAT 
GACCCTGCCC TACAGCCTCT GCTCATATCT TATGATAAAG CTGCGTCCAA GAGCATTGTC 
AACAACGGCC ACTCCTTTAA CGTTGAGTTT GATGACTCTC AGGACAATGC AGTGCTGAAA 
GGAGGACCCC TCAGTGACTC CTACAGATTG ATCCAGTTTC ACTTTCACTG GGGCTCATCT 
GATGGCCAGG GCTCTGAGCA CACTGTGAAC AAAAAAAAAT ATGCTGCAGA GCTTCACTTG 
GTTCACTGGA ACACCAAATA TGGGGACTTT GGAAAAGCTG TGCAGCAACC GGATGGATTG 
GCTGTTTTGG GTATTTTTTT GAAGATTGGA CCTGCCTCAC AAGGCCTTCA GAAAGTCCTT 
GAAGCACTGC ATTCCATTAA AACAAAGGGG AAGCGTGCGG CCTTTGCTAA CTTCGATCCT 
TGCTCCCTTC TTCCTGGAAA CTTGGACTAC TGGACATACC CTGGCTCTCT GACCACTCCG 
CCTCTGCTGG AATGTGTGAC CTGGATCGTG CTCAGGGAGC CCATTACTGT CAGCAGCGAG 
CAGATGTCTC ATTTCCGTAC GCTGAACTTC AATGAGGAGG GGGATGCTGA AGAAGCGATG 
GTGGACAACT GGCGTCCAGC TCAGCCGCTA AAGAATAGAA AGATCAAAGC GTCCTTTAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Car2 ( NM_009801.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033931.4 Mus musculus carbonic anhydrase 2 (Car2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13121
Clone ID Related Accession (Same CDS sequence)	NM_009801.4 , XM_006530050.1 , NM_001357334.1 , NM_009801.5
Accession Version	NM_009801.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Mus musculus(house mouse)
Product	carbonic anhydrase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC123747.26, AK002333.1 and CD774528.1. On Oct 26, 2017 this sequence version replaced NM_009801.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC055291.1, SRR1660815.11432.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCACC ACTGGGGATA CAGCAAGCAC AACGGACCAG AGAACTGGCA CAAGGACTTC 
CCCATTGCCA ATGGAGACCG GCAGTCCCCT GTGGACATTG ACACAGCAAC TGCCCAGCAT 
GACCCTGCCC TACAGCCTCT GCTCATATCT TATGATAAAG CTGCGTCCAA GAGCATTGTC 
AACAACGGCC ACTCCTTTAA CGTTGAGTTT GATGACTCTC AGGACAATGC AGTGCTGAAA 
GGAGGACCCC TCAGTGACTC CTACAGATTG ATCCAGTTTC ACTTTCACTG GGGCTCATCT 
GATGGCCAGG GCTCTGAGCA CACTGTGAAC AAAAAAAAAT ATGCTGCAGA GCTTCACTTG 
GTTCACTGGA ACACCAAATA TGGGGACTTT GGAAAAGCTG TGCAGCAACC GGATGGATTG 
GCTGTTTTGG GTATTTTTTT GAAGATTGGA CCTGCCTCAC AAGGCCTTCA GAAAGTCCTT 
GAAGCACTGC ATTCCATTAA AACAAAGGGG AAGCGTGCGG CCTTTGCTAA CTTCGATCCT 
TGCTCCCTTC TTCCTGGAAA CTTGGACTAC TGGACATACC CTGGCTCTCT GACCACTCCG 
CCTCTGCTGG AATGTGTGAC CTGGATCGTG CTCAGGGAGC CCATTACTGT CAGCAGCGAG 
CAGATGTCTC ATTTCCGTAC GCTGAACTTC AATGAGGAGG GGGATGCTGA AGAAGCGATG 
GTGGACAACT GGCGTCCAGC TCAGCCGCTA AAGAATAGAA AGATCAAAGC GTCCTTTAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033931.4
CDS	466..1248
Translation	MSHHWGYSKHNGPENWHKDFPIANGDRQSPVDIDTATAQHDPALQPLLISYDKAASKSIVNNGHSFNVEFDDSQDNAVLKGGPLSDSYRLIQFHFHWGSSDGQGSEHTVNKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKIGPASQGLQKVLEALHSIKTKGKRAAFANFDPCSLLPGNLDYWTYPGSLTTPPLLECVTWIVLREPITVSSEQMSHFRTLNFNEEGDAEEAMVDNWRPAQPLKNRKIKASFK

Target ORF information:

RefSeq Version	NM_009801.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbonic anhydrase 2 (Car2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009801.5

ORF Insert Sequence:

ATGTCCCACC ACTGGGGATA CAGCAAGCAC AACGGACCAG AGAACTGGCA CAAGGACTTC 
CCCATTGCCA ATGGAGACCG GCAGTCCCCT GTGGACATTG ACACAGCAAC TGCCCAGCAT 
GACCCTGCCC TACAGCCTCT GCTCATATCT TATGATAAAG CTGCGTCCAA GAGCATTGTC 
AACAACGGCC ACTCCTTTAA CGTTGAGTTT GATGACTCTC AGGACAATGC AGTGCTGAAA 
GGAGGACCCC TCAGTGACTC CTACAGATTG ATCCAGTTTC ACTTTCACTG GGGCTCATCT 
GATGGCCAGG GCTCTGAGCA CACTGTGAAC AAAAAAAAAT ATGCTGCAGA GCTTCACTTG 
GTTCACTGGA ACACCAAATA TGGGGACTTT GGAAAAGCTG TGCAGCAACC GGATGGATTG 
GCTGTTTTGG GTATTTTTTT GAAGATTGGA CCTGCCTCAC AAGGCCTTCA GAAAGTCCTT 
GAAGCACTGC ATTCCATTAA AACAAAGGGG AAGCGTGCGG CCTTTGCTAA CTTCGATCCT 
TGCTCCCTTC TTCCTGGAAA CTTGGACTAC TGGACATACC CTGGCTCTCT GACCACTCCG 
CCTCTGCTGG AATGTGTGAC CTGGATCGTG CTCAGGGAGC CCATTACTGT CAGCAGCGAG 
CAGATGTCTC ATTTCCGTAC GCTGAACTTC AATGAGGAGG GGGATGCTGA AGAAGCGATG 
GTGGACAACT GGCGTCCAGC TCAGCCGCTA AAGAATAGAA AGATCAAAGC GTCCTTTAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Car2 ( XM_006530050.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530113.1 Mus musculus carbonic anhydrase 2 (Car2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13121
Clone ID Related Accession (Same CDS sequence)	NM_009801.4 , XM_006530050.1 , NM_001357334.1 , NM_009801.5
Accession Version	XM_006530050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	carbonic anhydrase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCACC ACTGGGGATA CAGCAAGCAC AACGGACCAG AGAACTGGCA CAAGGACTTC 
CCCATTGCCA ATGGAGACCG GCAGTCCCCT GTGGACATTG ACACAGCAAC TGCCCAGCAT 
GACCCTGCCC TACAGCCTCT GCTCATATCT TATGATAAAG CTGCGTCCAA GAGCATTGTC 
AACAACGGCC ACTCCTTTAA CGTTGAGTTT GATGACTCTC AGGACAATGC AGTGCTGAAA 
GGAGGACCCC TCAGTGACTC CTACAGATTG ATCCAGTTTC ACTTTCACTG GGGCTCATCT 
GATGGCCAGG GCTCTGAGCA CACTGTGAAC AAAAAAAAAT ATGCTGCAGA GCTTCACTTG 
GTTCACTGGA ACACCAAATA TGGGGACTTT GGAAAAGCTG TGCAGCAACC GGATGGATTG 
GCTGTTTTGG GTATTTTTTT GAAGATTGGA CCTGCCTCAC AAGGCCTTCA GAAAGTCCTT 
GAAGCACTGC ATTCCATTAA AACAAAGGGG AAGCGTGCGG CCTTTGCTAA CTTCGATCCT 
TGCTCCCTTC TTCCTGGAAA CTTGGACTAC TGGACATACC CTGGCTCTCT GACCACTCCG 
CCTCTGCTGG AATGTGTGAC CTGGATCGTG CTCAGGGAGC CCATTACTGT CAGCAGCGAG 
CAGATGTCTC ATTTCCGTAC GCTGAACTTC AATGAGGAGG GGGATGCTGA AGAAGCGATG 
GTGGACAACT GGCGTCCAGC TCAGCCGCTA AAGAATAGAA AGATCAAAGC GTCCTTTAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530113.1
CDS	238..1020
Translation	MSHHWGYSKHNGPENWHKDFPIANGDRQSPVDIDTATAQHDPALQPLLISYDKAASKSIVNNGHSFNVEFDDSQDNAVLKGGPLSDSYRLIQFHFHWGSSDGQGSEHTVNKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKIGPASQGLQKVLEALHSIKTKGKRAAFANFDPCSLLPGNLDYWTYPGSLTTPPLLECVTWIVLREPITVSSEQMSHFRTLNFNEEGDAEEAMVDNWRPAQPLKNRKIKASFK

Target ORF information:

RefSeq Version	XM_006530050.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus carbonic anhydrase 2 (Car2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530050.1

ORF Insert Sequence:

ATGTCCCACC ACTGGGGATA CAGCAAGCAC AACGGACCAG AGAACTGGCA CAAGGACTTC 
CCCATTGCCA ATGGAGACCG GCAGTCCCCT GTGGACATTG ACACAGCAAC TGCCCAGCAT 
GACCCTGCCC TACAGCCTCT GCTCATATCT TATGATAAAG CTGCGTCCAA GAGCATTGTC 
AACAACGGCC ACTCCTTTAA CGTTGAGTTT GATGACTCTC AGGACAATGC AGTGCTGAAA 
GGAGGACCCC TCAGTGACTC CTACAGATTG ATCCAGTTTC ACTTTCACTG GGGCTCATCT 
GATGGCCAGG GCTCTGAGCA CACTGTGAAC AAAAAAAAAT ATGCTGCAGA GCTTCACTTG 
GTTCACTGGA ACACCAAATA TGGGGACTTT GGAAAAGCTG TGCAGCAACC GGATGGATTG 
GCTGTTTTGG GTATTTTTTT GAAGATTGGA CCTGCCTCAC AAGGCCTTCA GAAAGTCCTT 
GAAGCACTGC ATTCCATTAA AACAAAGGGG AAGCGTGCGG CCTTTGCTAA CTTCGATCCT 
TGCTCCCTTC TTCCTGGAAA CTTGGACTAC TGGACATACC CTGGCTCTCT GACCACTCCG 
CCTCTGCTGG AATGTGTGAC CTGGATCGTG CTCAGGGAGC CCATTACTGT CAGCAGCGAG 
CAGATGTCTC ATTTCCGTAC GCTGAACTTC AATGAGGAGG GGGATGCTGA AGAAGCGATG 
GTGGACAACT GGCGTCCAGC TCAGCCGCTA AAGAATAGAA AGATCAAAGC GTCCTTTAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function:
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(6)2562-2575(2017 06)
Chatel B,Bendahan D,Hourd? C,Pellerin L,Lengacher S,Magistretti P,Le Fur Y,Vilmen C,Bernard M,Messonnier LA

Transcription factor
eLife6(2017 Jun)
Werth M,Schmidt-Ott KM,Leete T,Qiu A,Hinze C,Viltard M,Paragas N,Shawber CJ,Yu W,Lee P,Chen X,Sarkar A,Mu W,Rittenberg A,Lin CS,Kitajewski J,Al-Awqati Q,Barasch J

Elf5 is a principal cell lineage specific transcription factor in the kidney that contributes to Aqp2 and Avpr2 gene expression.
Developmental biology424(1)77-89(2017 04)
Grassmeyer J,Mukherjee M,deRiso J,Hettinger C,Bailey M,Sinha S,Visvader JE,Zhao H,Fogarty E,Surendran K

Deficiency of Carbonic Anhydrase II Results in a Urinary Concentrating Defect.
Frontiers in physiology81108(2017)
Krishnan D,Pan W,Beggs MR,Trepiccione F,Chambrey R,Eladari D,Cordat E,Dimke H,Alexander RT

Basal cells express functional TRPV4 channels in the mouse nasal epithelium.
Biochemistry and biophysics reports4169-174(2015 Dec)
Ueda T,Hoshikawa M,Shibata Y,Kumamoto N,Ugawa S

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Car2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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