Bop1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bop1
Entrez Gene ID	12181
Full Name	block of proliferation 1
Synonyms	AU020183,AW146150,D18861,Erb1p,Kiaa0124,mKIAA0124
General protein information	Preferred Names block of proliferation 1 Names ribosome biogenesis protein BOP1 block of proliferation 1 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 D3\|15 35.91 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013481.1	NP_038509.1	ribosome biogenesis protein BOP1
NM_013481.2	NP_038509.1	ribosome biogenesis protein BOP1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Bop1	NP_001019421.1
Gallus gallus (chicken)	BOP1	XP_004950532.1
Anopheles gambiae (African malaria mosquito)	BOP1_ANOGA	XP_308633.4
Xenopus tropicalis (tropical clawed frog)	bop1	NP_001096309.1
	LOC714024	XP_002805572.1
Danio rerio (zebrafish)	bop1	NP_001071203.1
Caenorhabditis elegans (roundworm)	CELE_Y48B6A.1	NP_496956.1
Kluyveromyces lactis	KLLA0F26653g	XP_456266.1
Eremothecium gossypii	AGOS_ABR101C	NP_983048.2
Neurospora crassa	NCU03321	XP_964498.1
Arabidopsis thaliana (thale cress)	AT2G40360	NP_181567.3
Pan troglodytes (chimpanzee)	BOP1	XP_003312026.2
Canis lupus familiaris (dog)	BOP1	XP_003431868.2
Homo sapiens (human)	BOP1	NP_056016.1
Saccharomyces cerevisiae (baker's yeast)	ERB1	NP_013764.1
Schizosaccharomyces pombe (fission yeast)	SPBC4F6.13c	NP_596113.2
Magnaporthe oryzae (rice blast fungus)	MGG_09313	XP_003709965.1
Oryza sativa (rice)	Os07g0435400	NP_001059502.1
Bos taurus (cattle)	BOP1	XP_002692629.2
Mus musculus (house mouse)	Bop1	NP_038509.1
Drosophila melanogaster (fruit fly)	CG5033	NP_611270.1

Gene Ontology
Bibliography

Related articles in PubMed

New Wnt/?-catenin target genes promote experimental metastasis and migration of colorectal cancer cells through different signals.
Qi J, Yu Y, Akilli ?zt?rk ?, Holland JD, Besser D, Fritzmann J, Wulf-Goldenberg A, Eckert K, Fichtner I, Birchmeier W
Gut65(10)1690-701(2016 Oct)

Constraint and divergence of global gene expression in the mammalian embryo.
Spies N, Smith CL, Rodriguez JM, Baker JC, Batzoglou S, Sidow A
eLife4e05538(2015 Apr)

Estrogen down-regulation of the Scx gene is mediated by the opposing strand-overlapping gene Bop1.
Arao Y, Carpenter K, Hewitt S, Korach KS
The Journal of biological chemistry285(7)4806-14(2010 Feb)

Nucleophosmin serves as a rate-limiting nuclear export chaperone for the Mammalian ribosome.
Maggi LB Jr, Kuchenruether M, Dadey DY, Schwope RM, Grisendi S, Townsend RR, Pandolfi PP, Weber JD
Molecular and cellular biology28(23)7050-65(2008 Dec)

Interdependence of Pes1, Bop1, and WDR12 controls nucleolar localization and assembly of the PeBoW complex required for maturation of the 60S ribosomal subunit.
Rohrmoser M, H?lzel M, Grimm T, Malamoussi A, Harasim T, Orban M, Pfisterer I, Gruber-Eber A, Kremmer E, Eick D
Molecular and cellular biology27(10)3682-94(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

New direct Wnt/beta-catenin target gene Bop1, which induced epithelial-mesenchymal transition, cell migration and experimental metastasis of Colorectal cancer cells was identified.
Title: New Wnt/?-catenin target genes promote experimental metastasis and migration of colorectal cancer cells through different signals.

Murine fibroblasts expressing Scx mRNA from an exogenous Scx mini-gene indicated that the accumulation of Bop1 intronic RNA impairs the Scx gene expression in a trans-acting manner, which resulted in the reduction of the Scx mRNA level.
Title: Estrogen down-regulation of the Scx gene is mediated by the opposing strand-overlapping gene Bop1.

Results describe the role of PeBoW-specific proteins Pes1, Bop1, and WDR12 in complex assembly and stability, nucleolar transport, and pre-ribosome association.
Title: Interdependence of Pes1, Bop1, and WDR12 controls nucleolar localization and assembly of the PeBoW complex required for maturation of the 60S ribosomal subunit.

Bop1 form a stable complex with a novel member, WDR12 (PeBoW complex).
Title: Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation.

inactivation of inhibits multiple steps in pre-rRNA processing and blocks cell cycle progression
Title: Functional inactivation of the mouse nucleolar protein Bop1 inhibits multiple steps in pre-rRNA processing and blocks cell cycle progression.

The following Bop1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bop1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09458	NM_013481.2 Latest version!	Mus musculus block of proliferation 1 (Bop1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$433.30 $619.00
View Old Accession Versions >>
OMu09458	NM_013481.1	Mus musculus block of proliferation 1 (Bop1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$433.30 $619.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bop1 ( NM_013481.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038509.1 Mus musculus block of proliferation 1 (Bop1), mRNA.

Price & Availability↑

CloneID	OMu09458
Clone ID Related Accession (Same CDS sequence)	NM_013481.1 , NM_013481.2
Accession Version	NM_013481.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-10
Organism	Mus musculus(house mouse)
Product	ribosome biogenesis protein BOP1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U77415.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U77415.1, AK144095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGGG CGTGTGGTAA GCCCCACATG TCACCGGCAT CGCTGCCGGG GAAACGACGT 
TTGGAACCTG ATCAGGAGCT GCAGATACAA GAGCCTCCTC TCCTCAGCGA TCCTGACTCC 
AGTCTCTCTG ACAGCGAGGA GAGTGTGTTT TCAGGCCTCG AAGATTTGGG CAGTGACTCG 
AGTGAGGAAG ACACTGAAGG AGTGGCCGGA TCCAGCGGTG ACGAAGACAA CCATAGGGCA 
GAGGAGACCT CTGAGGAGCT GGCACAGGCT GCCCCTCTTT GCTCAAGGAC AGAGGAAGCA 
GGTGCTCTGG CCCAAGATGA ATATGAAGAG GACAGCTCTG ATGAGGAGGA CATTCGGAAC 
ACTGTGGGCA ATGTGCCCCT GGCATGGTAC GATGAATTCC CACATGTGGG TTATGACCTG 
GATGGCAAAC GTATCTACAA GCCCCTGCGG ACACGAGATG AGCTCGACCA GTTTCTGGAC 
AAAATGGATG ACCCAGATTT CTGGCGCACT GTGCAAGACA AGATGACAGG GCGTGATCTG 
CGGCTAACTG ATGAGCAGGT TGCCCTGGTA CACCGGCTTC AGAGAGGCCA GTTCGGAGAT 
TCAGGCTTCA ATCCCTATGA GCCAGCTGTG GACTTCTTCA GCGGTGACAT CATGATCCAC 
CCTGTGACCA ACCGCCCAGC TGACAAGCGT AGTTTCATCC CATCCCTAGT TGAAAAGGAG 
AAGGTGTCTC GAATGGTACA TGCCATCAAG ATGGGGTGGA TCAAGCCTCG ACGGCCCCAC 
GACCCCACCC CTAGCTTCTA TGACCTGTGG GCCCAGGAGG ATCCGAATGC TGTGTTGGGA 
CGCCACAAGA TGCATGTGCC TGCCCCCAAG CTGGCCTTGC CTGGCCATGC CGAGTCTTAC 
AACCCACCTC CCGAGTACCT GCCCACTGAG GAGGAGCGCT CGGCGTGGAT GCAGCAGGAG 
CCTGTGGAGA GGAAGCTTAA CTTCTTGCCA CAGAAATTCC CCAGCTTGAG GACAGTGCCT 
GCTTACAGCC GCTTCATCCA GGAGCGTTTC GAGCGCTGCC TCGATTTGTA TCTGTGCCCA 
CGGCAACGCA AGATGAGGGT GAATGTGGAC CCCGAAGACC TCATCCCCAA ACTCCCTCGG 
CCAAGAGACC TTCAGCCTTT CCCTGTGTGC CAGGCCCTCG TCTACAGGGG CCACAGCGAC 
CTTGTCCGTT GCCTCAGTGT CTCGCCAGGG GGCCAGTGGC TAGCTTCAGG TTCAGACGAT 
GGCACGTTGA AGCTCTGGGA GGTGGCCACT GCCCGCTGTA TGAAGACTGT ACATGTTGGA 
GGTGTGGTGC GGAGCATCGC CTGGAACCCC AATCCTACCA TATGCCTGGT AGCTGCTGCC 
ATGGATGATG CCGTGTTGCT GCTGAACCCA GCCCTGGGGG ACCGGCTGCT GGTGGGCAGC 
ACAGACCAGC TGCTGGAAGC CTTCACTCCA CCTGAGGAGC CAGCTCTGCA GCCTGCCCGG 
TGGCTAGAGG TCTCGGAGGA AGAGCACCAG AGGGGTCTGC GCCTACGCAT CTGCCACAGC 
AAACCAGTGA CACAGGTGAC CTGGCATGGG CGAGGGGACT ATTTGGCGGT GGTGCTGTCT 
AGTCAAGAGC ACACGCAAGT GCTGCTTCAC CAGGTGAGCA GGAGGCGCAG CCAGAGCCCG 
TTCCGCCGCA GCCACGGGCA GGTGCAGTGT GTGGCCTTCC ATCCCTCCCG GCCCTTCCTG 
CTCGTGGCCT CCCAGCGCAG CATCCGCATT TACCACCTGC TGCGCCAGGA GCTAACCAAG 
AAGCTGATGC CCAACTGCAA GTGGGTGTCC AGCATGGCTG TACATCCAGC AGGTGACAAC 
ATCATCTGTG GCAGCTATGA CAGCAAACTG GTGTGGTTTG ACCTGGATCT TTCCACCAAG 
CCATACAAAG TGCTGAGGCA CCACAAGAAG GCCTTGCGGG CTGTGGCCTT CCACCCCCGA 
TACCCACTCT TTGCATCCGG CTCAGACGAC GGCAGTGTTA TCGTGTGCCA TGGCATGGTG 
TACAATGACC TGCTGCAGAA CCCATTGCTG GTGCCTGTCA AGGTGCTGAA AGGACACACC 
CTGACCCGAG ATCTGGGTGT TCTGGATGTG GCCTTCCACC CCACACAGCC GTGGGTCTTC 
TCCTCCGGGG CAGATGGCAC CATTCGACTC TTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038509.1
CDS	49..2247
Translation	MAGACGKPHMSPASLPGKRRLEPDQELQIQEPPLLSDPDSSLSDSEESVFSGLEDLGSDSSEEDTEGVAGSSGDEDNHRAEETSEELAQAAPLCSRTEEAGALAQDEYEEDSSDEEDIRNTVGNVPLAWYDEFPHVGYDLDGKRIYKPLRTRDELDQFLDKMDDPDFWRTVQDKMTGRDLRLTDEQVALVHRLQRGQFGDSGFNPYEPAVDFFSGDIMIHPVTNRPADKRSFIPSLVEKEKVSRMVHAIKMGWIKPRRPHDPTPSFYDLWAQEDPNAVLGRHKMHVPAPKLALPGHAESYNPPPEYLPTEEERSAWMQQEPVERKLNFLPQKFPSLRTVPAYSRFIQERFERCLDLYLCPRQRKMRVNVDPEDLIPKLPRPRDLQPFPVCQALVYRGHSDLVRCLSVSPGGQWLASGSDDGTLKLWEVATARCMKTVHVGGVVRSIAWNPNPTICLVAAAMDDAVLLLNPALGDRLLVGSTDQLLEAFTPPEEPALQPARWLEVSEEEHQRGLRLRICHSKPVTQVTWHGRGDYLAVVLSSQEHTQVLLHQVSRRRSQSPFRRSHGQVQCVAFHPSRPFLLVASQRSIRIYHLLRQELTKKLMPNCKWVSSMAVHPAGDNIICGSYDSKLVWFDLDLSTKPYKVLRHHKKALRAVAFHPRYPLFASGSDDGSVIVCHGMVYNDLLQNPLLVPVKVLKGHTLTRDLGVLDVAFHPTQPWVFSSGADGTIRLFS

Target ORF information:

RefSeq Version	NM_013481.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus block of proliferation 1 (Bop1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013481.1

ORF Insert Sequence:

ATGGCGGGGG CGTGTGGTAA GCCCCACATG TCACCGGCAT CGCTGCCGGG GAAACGACGT 
TTGGAACCTG ATCAGGAGCT GCAGATACAA GAGCCTCCTC TCCTCAGCGA TCCTGACTCC 
AGTCTCTCTG ACAGCGAGGA GAGTGTGTTT TCAGGCCTCG AAGATTTGGG CAGTGACTCG 
AGTGAGGAAG ACACTGAAGG AGTGGCCGGA TCCAGCGGTG ACGAAGACAA CCATAGGGCA 
GAGGAGACCT CTGAGGAGCT GGCACAGGCT GCCCCTCTTT GCTCAAGGAC AGAGGAAGCA 
GGTGCTCTGG CCCAAGATGA ATATGAAGAG GACAGCTCTG ATGAGGAGGA CATTCGGAAC 
ACTGTGGGCA ATGTGCCCCT GGCATGGTAC GATGAATTCC CACATGTGGG TTATGACCTG 
GATGGCAAAC GTATCTACAA GCCCCTGCGG ACACGAGATG AGCTCGACCA GTTTCTGGAC 
AAAATGGATG ACCCAGATTT CTGGCGCACT GTGCAAGACA AGATGACAGG GCGTGATCTG 
CGGCTAACTG ATGAGCAGGT TGCCCTGGTA CACCGGCTTC AGAGAGGCCA GTTCGGAGAT 
TCAGGCTTCA ATCCCTATGA GCCAGCTGTG GACTTCTTCA GCGGTGACAT CATGATCCAC 
CCTGTGACCA ACCGCCCAGC TGACAAGCGT AGTTTCATCC CATCCCTAGT TGAAAAGGAG 
AAGGTGTCTC GAATGGTACA TGCCATCAAG ATGGGGTGGA TCAAGCCTCG ACGGCCCCAC 
GACCCCACCC CTAGCTTCTA TGACCTGTGG GCCCAGGAGG ATCCGAATGC TGTGTTGGGA 
CGCCACAAGA TGCATGTGCC TGCCCCCAAG CTGGCCTTGC CTGGCCATGC CGAGTCTTAC 
AACCCACCTC CCGAGTACCT GCCCACTGAG GAGGAGCGCT CGGCGTGGAT GCAGCAGGAG 
CCTGTGGAGA GGAAGCTTAA CTTCTTGCCA CAGAAATTCC CCAGCTTGAG GACAGTGCCT 
GCTTACAGCC GCTTCATCCA GGAGCGTTTC GAGCGCTGCC TCGATTTGTA TCTGTGCCCA 
CGGCAACGCA AGATGAGGGT GAATGTGGAC CCCGAAGACC TCATCCCCAA ACTCCCTCGG 
CCAAGAGACC TTCAGCCTTT CCCTGTGTGC CAGGCCCTCG TCTACAGGGG CCACAGCGAC 
CTTGTCCGTT GCCTCAGTGT CTCGCCAGGG GGCCAGTGGC TAGCTTCAGG TTCAGACGAT 
GGCACGTTGA AGCTCTGGGA GGTGGCCACT GCCCGCTGTA TGAAGACTGT ACATGTTGGA 
GGTGTGGTGC GGAGCATCGC CTGGAACCCC AATCCTACCA TATGCCTGGT AGCTGCTGCC 
ATGGATGATG CCGTGTTGCT GCTGAACCCA GCCCTGGGGG ACCGGCTGCT GGTGGGCAGC 
ACAGACCAGC TGCTGGAAGC CTTCACTCCA CCTGAGGAGC CAGCTCTGCA GCCTGCCCGG 
TGGCTAGAGG TCTCGGAGGA AGAGCACCAG AGGGGTCTGC GCCTACGCAT CTGCCACAGC 
AAACCAGTGA CACAGGTGAC CTGGCATGGG CGAGGGGACT ATTTGGCGGT GGTGCTGTCT 
AGTCAAGAGC ACACGCAAGT GCTGCTTCAC CAGGTGAGCA GGAGGCGCAG CCAGAGCCCG 
TTCCGCCGCA GCCACGGGCA GGTGCAGTGT GTGGCCTTCC ATCCCTCCCG GCCCTTCCTG 
CTCGTGGCCT CCCAGCGCAG CATCCGCATT TACCACCTGC TGCGCCAGGA GCTAACCAAG 
AAGCTGATGC CCAACTGCAA GTGGGTGTCC AGCATGGCTG TACATCCAGC AGGTGACAAC 
ATCATCTGTG GCAGCTATGA CAGCAAACTG GTGTGGTTTG ACCTGGATCT TTCCACCAAG 
CCATACAAAG TGCTGAGGCA CCACAAGAAG GCCTTGCGGG CTGTGGCCTT CCACCCCCGA 
TACCCACTCT TTGCATCCGG CTCAGACGAC GGCAGTGTTA TCGTGTGCCA TGGCATGGTG 
TACAATGACC TGCTGCAGAA CCCATTGCTG GTGCCTGTCA AGGTGCTGAA AGGACACACC 
CTGACCCGAG ATCTGGGTGT TCTGGATGTG GCCTTCCACC CCACACAGCC GTGGGTCTTC 
TCCTCCGGGG CAGATGGCAC CATTCGACTC TTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bop1 ( NM_013481.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038509.1 Mus musculus block of proliferation 1 (Bop1), mRNA.

Price & Availability↑

CloneID	OMu09458
Clone ID Related Accession (Same CDS sequence)	NM_013481.1 , NM_013481.2
Accession Version	NM_013481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Mus musculus(house mouse)
Product	ribosome biogenesis protein BOP1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK079627.1 and AV349722.1. On Feb 5, 2019 this sequence version replaced NM_013481.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7652917.702019.1, AK079627.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGGG CGTGTGGTAA GCCCCACATG TCACCGGCAT CGCTGCCGGG GAAACGACGT 
TTGGAACCTG ATCAGGAGCT GCAGATACAA GAGCCTCCTC TCCTCAGCGA TCCTGACTCC 
AGTCTCTCTG ACAGCGAGGA GAGTGTGTTT TCAGGCCTCG AAGATTTGGG CAGTGACTCG 
AGTGAGGAAG ACACTGAAGG AGTGGCCGGA TCCAGCGGTG ACGAAGACAA CCATAGGGCA 
GAGGAGACCT CTGAGGAGCT GGCACAGGCT GCCCCTCTTT GCTCAAGGAC AGAGGAAGCA 
GGTGCTCTGG CCCAAGATGA ATATGAAGAG GACAGCTCTG ATGAGGAGGA CATTCGGAAC 
ACTGTGGGCA ATGTGCCCCT GGCATGGTAC GATGAATTCC CACATGTGGG TTATGACCTG 
GATGGCAAAC GTATCTACAA GCCCCTGCGG ACACGAGATG AGCTCGACCA GTTTCTGGAC 
AAAATGGATG ACCCAGATTT CTGGCGCACT GTGCAAGACA AGATGACAGG GCGTGATCTG 
CGGCTAACTG ATGAGCAGGT TGCCCTGGTA CACCGGCTTC AGAGAGGCCA GTTCGGAGAT 
TCAGGCTTCA ATCCCTATGA GCCAGCTGTG GACTTCTTCA GCGGTGACAT CATGATCCAC 
CCTGTGACCA ACCGCCCAGC TGACAAGCGT AGTTTCATCC CATCCCTAGT TGAAAAGGAG 
AAGGTGTCTC GAATGGTACA TGCCATCAAG ATGGGGTGGA TCAAGCCTCG ACGGCCCCAC 
GACCCCACCC CTAGCTTCTA TGACCTGTGG GCCCAGGAGG ATCCGAATGC TGTGTTGGGA 
CGCCACAAGA TGCATGTGCC TGCCCCCAAG CTGGCCTTGC CTGGCCATGC CGAGTCTTAC 
AACCCACCTC CCGAGTACCT GCCCACTGAG GAGGAGCGCT CGGCGTGGAT GCAGCAGGAG 
CCTGTGGAGA GGAAGCTTAA CTTCTTGCCA CAGAAATTCC CCAGCTTGAG GACAGTGCCT 
GCTTACAGCC GCTTCATCCA GGAGCGTTTC GAGCGCTGCC TCGATTTGTA TCTGTGCCCA 
CGGCAACGCA AGATGAGGGT GAATGTGGAC CCCGAAGACC TCATCCCCAA ACTCCCTCGG 
CCAAGAGACC TTCAGCCTTT CCCTGTGTGC CAGGCCCTCG TCTACAGGGG CCACAGCGAC 
CTTGTCCGTT GCCTCAGTGT CTCGCCAGGG GGCCAGTGGC TAGCTTCAGG TTCAGACGAT 
GGCACGTTGA AGCTCTGGGA GGTGGCCACT GCCCGCTGTA TGAAGACTGT ACATGTTGGA 
GGTGTGGTGC GGAGCATCGC CTGGAACCCC AATCCTACCA TATGCCTGGT AGCTGCTGCC 
ATGGATGATG CCGTGTTGCT GCTGAACCCA GCCCTGGGGG ACCGGCTGCT GGTGGGCAGC 
ACAGACCAGC TGCTGGAAGC CTTCACTCCA CCTGAGGAGC CAGCTCTGCA GCCTGCCCGG 
TGGCTAGAGG TCTCGGAGGA AGAGCACCAG AGGGGTCTGC GCCTACGCAT CTGCCACAGC 
AAACCAGTGA CACAGGTGAC CTGGCATGGG CGAGGGGACT ATTTGGCGGT GGTGCTGTCT 
AGTCAAGAGC ACACGCAAGT GCTGCTTCAC CAGGTGAGCA GGAGGCGCAG CCAGAGCCCG 
TTCCGCCGCA GCCACGGGCA GGTGCAGTGT GTGGCCTTCC ATCCCTCCCG GCCCTTCCTG 
CTCGTGGCCT CCCAGCGCAG CATCCGCATT TACCACCTGC TGCGCCAGGA GCTAACCAAG 
AAGCTGATGC CCAACTGCAA GTGGGTGTCC AGCATGGCTG TACATCCAGC AGGTGACAAC 
ATCATCTGTG GCAGCTATGA CAGCAAACTG GTGTGGTTTG ACCTGGATCT TTCCACCAAG 
CCATACAAAG TGCTGAGGCA CCACAAGAAG GCCTTGCGGG CTGTGGCCTT CCACCCCCGA 
TACCCACTCT TTGCATCCGG CTCAGACGAC GGCAGTGTTA TCGTGTGCCA TGGCATGGTG 
TACAATGACC TGCTGCAGAA CCCATTGCTG GTGCCTGTCA AGGTGCTGAA AGGACACACC 
CTGACCCGAG ATCTGGGTGT TCTGGATGTG GCCTTCCACC CCACACAGCC GTGGGTCTTC 
TCCTCCGGGG CAGATGGCAC CATTCGACTC TTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038509.1
CDS	29..2227
Translation	MAGACGKPHMSPASLPGKRRLEPDQELQIQEPPLLSDPDSSLSDSEESVFSGLEDLGSDSSEEDTEGVAGSSGDEDNHRAEETSEELAQAAPLCSRTEEAGALAQDEYEEDSSDEEDIRNTVGNVPLAWYDEFPHVGYDLDGKRIYKPLRTRDELDQFLDKMDDPDFWRTVQDKMTGRDLRLTDEQVALVHRLQRGQFGDSGFNPYEPAVDFFSGDIMIHPVTNRPADKRSFIPSLVEKEKVSRMVHAIKMGWIKPRRPHDPTPSFYDLWAQEDPNAVLGRHKMHVPAPKLALPGHAESYNPPPEYLPTEEERSAWMQQEPVERKLNFLPQKFPSLRTVPAYSRFIQERFERCLDLYLCPRQRKMRVNVDPEDLIPKLPRPRDLQPFPVCQALVYRGHSDLVRCLSVSPGGQWLASGSDDGTLKLWEVATARCMKTVHVGGVVRSIAWNPNPTICLVAAAMDDAVLLLNPALGDRLLVGSTDQLLEAFTPPEEPALQPARWLEVSEEEHQRGLRLRICHSKPVTQVTWHGRGDYLAVVLSSQEHTQVLLHQVSRRRSQSPFRRSHGQVQCVAFHPSRPFLLVASQRSIRIYHLLRQELTKKLMPNCKWVSSMAVHPAGDNIICGSYDSKLVWFDLDLSTKPYKVLRHHKKALRAVAFHPRYPLFASGSDDGSVIVCHGMVYNDLLQNPLLVPVKVLKGHTLTRDLGVLDVAFHPTQPWVFSSGADGTIRLFS

Target ORF information:

RefSeq Version	NM_013481.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus block of proliferation 1 (Bop1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013481.2

ORF Insert Sequence:

ATGGCGGGGG CGTGTGGTAA GCCCCACATG TCACCGGCAT CGCTGCCGGG GAAACGACGT 
TTGGAACCTG ATCAGGAGCT GCAGATACAA GAGCCTCCTC TCCTCAGCGA TCCTGACTCC 
AGTCTCTCTG ACAGCGAGGA GAGTGTGTTT TCAGGCCTCG AAGATTTGGG CAGTGACTCG 
AGTGAGGAAG ACACTGAAGG AGTGGCCGGA TCCAGCGGTG ACGAAGACAA CCATAGGGCA 
GAGGAGACCT CTGAGGAGCT GGCACAGGCT GCCCCTCTTT GCTCAAGGAC AGAGGAAGCA 
GGTGCTCTGG CCCAAGATGA ATATGAAGAG GACAGCTCTG ATGAGGAGGA CATTCGGAAC 
ACTGTGGGCA ATGTGCCCCT GGCATGGTAC GATGAATTCC CACATGTGGG TTATGACCTG 
GATGGCAAAC GTATCTACAA GCCCCTGCGG ACACGAGATG AGCTCGACCA GTTTCTGGAC 
AAAATGGATG ACCCAGATTT CTGGCGCACT GTGCAAGACA AGATGACAGG GCGTGATCTG 
CGGCTAACTG ATGAGCAGGT TGCCCTGGTA CACCGGCTTC AGAGAGGCCA GTTCGGAGAT 
TCAGGCTTCA ATCCCTATGA GCCAGCTGTG GACTTCTTCA GCGGTGACAT CATGATCCAC 
CCTGTGACCA ACCGCCCAGC TGACAAGCGT AGTTTCATCC CATCCCTAGT TGAAAAGGAG 
AAGGTGTCTC GAATGGTACA TGCCATCAAG ATGGGGTGGA TCAAGCCTCG ACGGCCCCAC 
GACCCCACCC CTAGCTTCTA TGACCTGTGG GCCCAGGAGG ATCCGAATGC TGTGTTGGGA 
CGCCACAAGA TGCATGTGCC TGCCCCCAAG CTGGCCTTGC CTGGCCATGC CGAGTCTTAC 
AACCCACCTC CCGAGTACCT GCCCACTGAG GAGGAGCGCT CGGCGTGGAT GCAGCAGGAG 
CCTGTGGAGA GGAAGCTTAA CTTCTTGCCA CAGAAATTCC CCAGCTTGAG GACAGTGCCT 
GCTTACAGCC GCTTCATCCA GGAGCGTTTC GAGCGCTGCC TCGATTTGTA TCTGTGCCCA 
CGGCAACGCA AGATGAGGGT GAATGTGGAC CCCGAAGACC TCATCCCCAA ACTCCCTCGG 
CCAAGAGACC TTCAGCCTTT CCCTGTGTGC CAGGCCCTCG TCTACAGGGG CCACAGCGAC 
CTTGTCCGTT GCCTCAGTGT CTCGCCAGGG GGCCAGTGGC TAGCTTCAGG TTCAGACGAT 
GGCACGTTGA AGCTCTGGGA GGTGGCCACT GCCCGCTGTA TGAAGACTGT ACATGTTGGA 
GGTGTGGTGC GGAGCATCGC CTGGAACCCC AATCCTACCA TATGCCTGGT AGCTGCTGCC 
ATGGATGATG CCGTGTTGCT GCTGAACCCA GCCCTGGGGG ACCGGCTGCT GGTGGGCAGC 
ACAGACCAGC TGCTGGAAGC CTTCACTCCA CCTGAGGAGC CAGCTCTGCA GCCTGCCCGG 
TGGCTAGAGG TCTCGGAGGA AGAGCACCAG AGGGGTCTGC GCCTACGCAT CTGCCACAGC 
AAACCAGTGA CACAGGTGAC CTGGCATGGG CGAGGGGACT ATTTGGCGGT GGTGCTGTCT 
AGTCAAGAGC ACACGCAAGT GCTGCTTCAC CAGGTGAGCA GGAGGCGCAG CCAGAGCCCG 
TTCCGCCGCA GCCACGGGCA GGTGCAGTGT GTGGCCTTCC ATCCCTCCCG GCCCTTCCTG 
CTCGTGGCCT CCCAGCGCAG CATCCGCATT TACCACCTGC TGCGCCAGGA GCTAACCAAG 
AAGCTGATGC CCAACTGCAA GTGGGTGTCC AGCATGGCTG TACATCCAGC AGGTGACAAC 
ATCATCTGTG GCAGCTATGA CAGCAAACTG GTGTGGTTTG ACCTGGATCT TTCCACCAAG 
CCATACAAAG TGCTGAGGCA CCACAAGAAG GCCTTGCGGG CTGTGGCCTT CCACCCCCGA 
TACCCACTCT TTGCATCCGG CTCAGACGAC GGCAGTGTTA TCGTGTGCCA TGGCATGGTG 
TACAATGACC TGCTGCAGAA CCCATTGCTG GTGCCTGTCA AGGTGCTGAA AGGACACACC 
CTGACCCGAG ATCTGGGTGT TCTGGATGTG GCCTTCCACC CCACACAGCC GTGGGTCTTC 
TCCTCCGGGG CAGATGGCAC CATTCGACTC TTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

New Wnt/?-catenin target genes promote experimental metastasis and migration of colorectal cancer cells through different signals.
Gut65(10)1690-701(2016 Oct)
Qi J,Yu Y,Akilli ?zt?rk ?,Holland JD,Besser D,Fritzmann J,Wulf-Goldenberg A,Eckert K,Fichtner I,Birchmeier W

Constraint and divergence of global gene expression in the mammalian embryo.
eLife4e05538(2015 Apr)
Spies N,Smith CL,Rodriguez JM,Baker JC,Batzoglou S,Sidow A

Estrogen down-regulation of the Scx gene is mediated by the opposing strand-overlapping gene Bop1.
The Journal of biological chemistry285(7)4806-14(2010 Feb)
Arao Y,Carpenter K,Hewitt S,Korach KS

Nucleophosmin serves as a rate-limiting nuclear export chaperone for the Mammalian ribosome.
Molecular and cellular biology28(23)7050-65(2008 Dec)
Maggi LB Jr,Kuchenruether M,Dadey DY,Schwope RM,Grisendi S,Townsend RR,Pandolfi PP,Weber JD

Interdependence of Pes1, Bop1, and WDR12 controls nucleolar localization and assembly of the PeBoW complex required for maturation of the 60S ribosomal subunit.
Molecular and cellular biology27(10)3682-94(2007 May)
Rohrmoser M,H?lzel M,Grimm T,Malamoussi A,Harasim T,Orban M,Pfisterer I,Gruber-Eber A,Kremmer E,Eick D

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Bop1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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