Bag1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bag1
Entrez Gene ID	12017
Full Name	BCL2-associated athanogene 1
Synonyms	BAG-1,Rap46
General protein information	Preferred Names BCL2-associated athanogene 1 Names BAG family molecular chaperone regulator 1 Bcl-2-binding protein bcl-2-associated athanogene 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 A5
Summary	The oncogene Bcl2 encodes a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to Bcl2 protein and is referred to as Bcl2-associated athanogene. It enhances the anti-apoptotic effects of Bcl2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least two protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site and an alternative, downstream, AUG translation initiation site. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001171739.1	NP_001165210.1	BAG family molecular chaperone regulator 1 isoform 1S
NM_009736.3	NP_033866.4	BAG family molecular chaperone regulator 1 isoform 1L

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BAG1	XP_520528.3
Canis lupus familiaris (dog)	BAG1	XP_005627044.1
Bos taurus (cattle)	BAG1	NP_001069759.1
Rattus norvegicus (Norway rat)	Bag1	NP_001100117.2
	BAG1	XP_002800106.1
Gallus gallus (chicken)	BAG1	NP_001103162.1
Danio rerio (zebrafish)	bag1	NP_001092206.1
Xenopus tropicalis (tropical clawed frog)	bag1	XP_002939543.2
Mus musculus (house mouse)	Bag1	NP_033866.4
Homo sapiens (human)	BAG1	NP_004314.5

Gene Ontology
Bibliography

Related articles in PubMed

BAG-1L Protects SH-SY5Y Neuroblastoma Cells Against Hypoxia/Re-oxygenation Through Up-Regulating HSP70 and Activating PI3K/AKT Signaling Pathway.
Wang Y, Jia C, Li QS, Xie CY, Zhang N, Qu Y
Neurochemical research42(10)2861-2868(2017 Oct)

BAG-1M co-activates BACE1 transcription through NF-?B and accelerates A? production and memory deficit in Alzheimer's disease mouse model.
Shi Z, Hong Y, Zhang K, Wang J, Zheng L, Zhang Z, Hu Z, Han X, Han Y, Chen T, Yao Q, Cui H, Hong W
Biochimica et biophysica acta1863(9)2398-2407(2017 Sep)

Regulation of osteoblast development by Bcl-2-associated athanogene-1 (BAG-1).
Greenhough J, Papadakis ES, Cutress RI, Townsend PA, Oreffo RO, Tare RS
Scientific reports633504(2016 Sep)

The BAG-1 isoform BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells during activation of inflammatory signaling.
Mashukova A, Kozhekbaeva Z, Forteza R, Dulam V, Figueroa Y, Warren R, Salas PJ
Journal of cell science127(Pt 16)3568-77(2014 Aug)

Characterization of interactions between the anti-apoptotic protein BAG-1 and Hsc70 molecular chaperones.
Stuart JK, Myszka DG, Joss L, Mitchell RS, McDonald SM, Xie Z, Takayama S, Reed JC, Ely KR
The Journal of biological chemistry273(35)22506-14(1998 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The membrane form of heparin binding EGF-like growth factor (ProHB-EGF) is most likely able to trigger the nuclear translocation of BAG-1 in keeping its level high.
Title: Nuclear magnetic resonance structure of the cytoplasmic tail of heparin binding EGF-like growth factor (proHB-EGF-CT) complexed with the ubiquitin homology domain of Bcl-2-associated athanogene 1 from Mus musculus (mBAG-1-UBH).

Hemoxygenase 1 deficiency provoked diminished Bag-1 level upon LPS treatment
Title: HO-1 as modulator of the innate immune response in pregnancy.

this study first reported the solution structure of mBAG-1-UBH and the growth factor precursor-interacting motif on the protein.
Title: The NMR solution structure of the ubiquitin homology domain of Bcl-2-associated athanogene 1 (BAG-1-UBH) from Mus musculus.

The BAG-1 stabilizes F508del-CFTR by direct binding, probably competing out ubiquitin to partially avoid its proteasomal degradation.
Title: BAG-1 stabilizes mutant F508del-CFTR in a ubiquitin-like-domain-dependent manner.

Bag-1 proteins function as regulators of the action of selective transcription factors.
Title: Depletion of the cellular levels of Bag-1 proteins attenuates phorbol ester-induced downregulation of I?B? and nuclear accumulation of NF-?B.

The following Bag1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bag1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22791	NM_001171739.1 Latest version!	Mus musculus BCL2-associated athanogene 1 (Bag1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$216.30 $309.00
OMu22824	NM_009736.3 Latest version!	Mus musculus BCL2-associated athanogene 1 (Bag1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$237.30 $339.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bag1 ( NM_001171739.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001165210.1 Mus musculus BCL2-associated athanogene 1 (Bag1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22791
Clone ID Related Accession (Same CDS sequence)	NM_001171739.1
Accession Version	NM_001171739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	BAG family molecular chaperone regulator 1 isoform 1S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CX225744.1, AK009149.1, AK013611.1 and BQ031093.1. Transcript Variant: This transcript (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longer isoform (BAG-1L or p50) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (BAG-1S or p32) is derived from a downstream AUG start codon. The shorter isoform (1S) is represented in this RefSeq. CCDS Note: This CCDS ID represents the shortest mouse Bag1 isoform, known as BAG-1S or p32, as described in the literature, including PMIDs 9679980 and 9396724. This isoform initiates translation at an AUG start codon. Alternative translation initiation at an upstream non-AUG (CUG) start codon produces an isoform that is 136 aa longer at the N-terminus, known as BAG-1L or p50. The longer isoform is represented by CCDS 38713.1. Evidence in PMID:9396724 indicates that these isoforms may have distinct subcellular distributions, which may contribute to the multifunctionality of the protein. Alternative translation initiation resulting in isoforms with differential subcellular localization has also been demonstrated for the human ortholog. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003722.1, AK154469.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGA CCGAGGAGAT GGTCCAGACG GAGGAAATGG AAACACCCAG ACTCAGCGTG 
ATCGTCACCC ACAGCAATGA GAGGTATGAC CTTCTTGTTA CCCCACAGCA AGGTAACAGT 
GAGCCAGTTG TCCAAGACTT GGCTCAGCTT GTTGAAGAGG CCACAGGAGT TCCACTACCT 
TTTCAGAAGC TCATATTTAA GGGAAAATCT CTGAAAGAAA TGGAAACACC GTTGTCAGCA 
CTTGGAATGC AAAATGGTTG CCGAGTCATG TTAATTGGTG AAAAGAGCAA TCCAGAAGAA 
GAGGTTGAGT TAAAGAAGCT GAAAGATTTG GAGGTATCTG CAGAGAAGAT AGCTAACCAC 
CTGCAAGAAT TGAATAAAGA GCTTTCTGGC ATCCAGCAGG GTTTTCTGGC TAAGGAATTG 
CAAGCGGAGG CTCTCTGCAA ACTTGATAGG AAAGTAAAAG CAACAATTGA GCAATTCATG 
AAGATCTTGG AGGAGATTGA CACAATGGTC CTACCAGAAC AATTTAAAGA CAGCAGGCTA 
AAAAGGAAGA ATTTGGTGAA AAAGGTTCAG GTGTTCTTAG CAGAGTGTGA CACAGTGGAG 
CAATACATCT GCCAAGAGAC AGAGCGGCTG CAGTCTACAA ACTTGGCCCT GGCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165210.1
CDS	501..1160
Translation	MAKTEEMVQTEEMETPRLSVIVTHSNERYDLLVTPQQGNSEPVVQDLAQLVEEATGVPLPFQKLIFKGKSLKEMETPLSALGMQNGCRVMLIGEKSNPEEEVELKKLKDLEVSAEKIANHLQELNKELSGIQQGFLAKELQAEALCKLDRKVKATIEQFMKILEEIDTMVLPEQFKDSRLKRKNLVKKVQVFLAECDTVEQYICQETERLQSTNLALAE

Target ORF information:

RefSeq Version	NM_001171739.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BCL2-associated athanogene 1 (Bag1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171739.1

ORF Insert Sequence:

ATGGCCAAGA CCGAGGAGAT GGTCCAGACG GAGGAAATGG AAACACCCAG ACTCAGCGTG 
ATCGTCACCC ACAGCAATGA GAGGTATGAC CTTCTTGTTA CCCCACAGCA AGGTAACAGT 
GAGCCAGTTG TCCAAGACTT GGCTCAGCTT GTTGAAGAGG CCACAGGAGT TCCACTACCT 
TTTCAGAAGC TCATATTTAA GGGAAAATCT CTGAAAGAAA TGGAAACACC GTTGTCAGCA 
CTTGGAATGC AAAATGGTTG CCGAGTCATG TTAATTGGTG AAAAGAGCAA TCCAGAAGAA 
GAGGTTGAGT TAAAGAAGCT GAAAGATTTG GAGGTATCTG CAGAGAAGAT AGCTAACCAC 
CTGCAAGAAT TGAATAAAGA GCTTTCTGGC ATCCAGCAGG GTTTTCTGGC TAAGGAATTG 
CAAGCGGAGG CTCTCTGCAA ACTTGATAGG AAAGTAAAAG CAACAATTGA GCAATTCATG 
AAGATCTTGG AGGAGATTGA CACAATGGTC CTACCAGAAC AATTTAAAGA CAGCAGGCTA 
AAAAGGAAGA ATTTGGTGAA AAAGGTTCAG GTGTTCTTAG CAGAGTGTGA CACAGTGGAG 
CAATACATCT GCCAAGAGAC AGAGCGGCTG CAGTCTACAA ACTTGGCCCT GGCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bag1 ( NM_009736.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033866.4 Mus musculus BCL2-associated athanogene 1 (Bag1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22824
Clone ID Related Accession (Same CDS sequence)	NM_009736.3
Accession Version	NM_009736.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	BAG family molecular chaperone regulator 1 isoform 1L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CX225744.1, AK009149.1, AK013611.1 and BQ031093.1. On Jan 26, 2010 this sequence version replaced NM_009736.2. Transcript Variant: This transcript (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longer isoform (BAG-1L or p50) is derived from an upstream non-AUG (CUG) start codon, while the shorter isoform (BAG-1S or p32) is derived from a downstream AUG start codon. The longer isoform (1L) is represented in this RefSeq. CCDS Note: This CCDS ID represents the longest mouse Bag1 isoform, known as BAG-1L or p50, as described in the literature, including PMIDs 9679980 and 9396724. This isoform initiates translation at a non-AUG (CUG) start codon that is well-conserved and present in a strong Kozak context. Alternative translation initiation at a downstream AUG start codon produces an isoform that is 136 aa shorter at the N-terminus, known as BAG-1S or p32. The shorter isoform is represented by CCDS 51139.1. Evidence in PMID:9396724 indicates that these isoforms may have distinct subcellular distributions, which may contribute to the multifunctionality of the protein. Alternative translation initiation resulting in isoforms with differential subcellular localization has also been demonstrated for the human ortholog. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003722.1, AK154469.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGCGGGTC GCAGCGCCGC GCGGCGACCG CGAGGCGACC GGGAGCCGCT GGGACCCAGG 
CTGCGCGCCC CTCGGCCTGC CCGGGAGCCG CGCCAGTCGG AGTCCCGGGC CGAGCGGGGC 
TTGCCTCCCT CTCAGCGTTC CTCTGTGCGC AGTGCAGCCA GCGGGCATGA CCGATCCACC 
AGGGGCGCCG CCGCCGGCGC CTGCAAGCCG CGGGTGAAGA AGAAAGTCCG GCCCCGCTCT 
TCTCAGAGCG AGAAGGTAGG GAGCAGCAGC AGGGAGTTGA CTAGAAGTAA GAAAGTGACC 
CGTAGCAAGA ACGTGACCGG GACCCAGGTA GAGGAGGTGA CCAAGATCGA GGAGGCGACC 
CAAACCGAGG AAGTAACTGT GGCAGAAGAG GTGACCCAGA CCGACAACAT GGCCAAGACC 
GAGGAGATGG TCCAGACGGA GGAAATGGAA ACACCCAGAC TCAGCGTGAT CGTCACCCAC 
AGCAATGAGA GGTATGACCT TCTTGTTACC CCACAGCAAG GTAACAGTGA GCCAGTTGTC 
CAAGACTTGG CTCAGCTTGT TGAAGAGGCC ACAGGAGTTC CACTACCTTT TCAGAAGCTC 
ATATTTAAGG GAAAATCTCT GAAAGAAATG GAAACACCGT TGTCAGCACT TGGAATGCAA 
AATGGTTGCC GAGTCATGTT AATTGGTGAA AAGAGCAATC CAGAAGAAGA GGTTGAGTTA 
AAGAAGCTGA AAGATTTGGA GGTATCTGCA GAGAAGATAG CTAACCACCT GCAAGAATTG 
AATAAAGAGC TTTCTGGCAT CCAGCAGGGT TTTCTGGCTA AGGAATTGCA AGCGGAGGCT 
CTCTGCAAAC TTGATAGGAA AGTAAAAGCA ACAATTGAGC AATTCATGAA GATCTTGGAG 
GAGATTGACA CAATGGTCCT ACCAGAACAA TTTAAAGACA GCAGGCTAAA AAGGAAGAAT 
TTGGTGAAAA AGGTTCAGGT GTTCTTAGCA GAGTGTGACA CAGTGGAGCA ATACATCTGC 
CAAGAGACAG AGCGGCTGCA GTCTACAAAC TTGGCCCTGG CTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033866.4
CDS	93..1160
Translation	LAGRSAARRPRGDREPLGPRLRAPRPAREPRQSESRAERGLPPSQRSSVRSAASGHDRSTRGAAAGACKPRVKKKVRPRSSQSEKVGSSSRELTRSKKVTRSKNVTGTQVEEVTKIEEATQTEEVTVAEEVTQTDNMAKTEEMVQTEEMETPRLSVIVTHSNERYDLLVTPQQGNSEPVVQDLAQLVEEATGVPLPFQKLIFKGKSLKEMETPLSALGMQNGCRVMLIGEKSNPEEEVELKKLKDLEVSAEKIANHLQELNKELSGIQQGFLAKELQAEALCKLDRKVKATIEQFMKILEEIDTMVLPEQFKDSRLKRKNLVKKVQVFLAECDTVEQYICQETERLQSTNLALAE

Target ORF information:

RefSeq Version	NM_009736.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BCL2-associated athanogene 1 (Bag1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009736.3

ORF Insert Sequence:

CTGGCGGGTC GCAGCGCCGC GCGGCGACCG CGAGGCGACC GGGAGCCGCT GGGACCCAGG 
CTGCGCGCCC CTCGGCCTGC CCGGGAGCCG CGCCAGTCGG AGTCCCGGGC CGAGCGGGGC 
TTGCCTCCCT CTCAGCGTTC CTCTGTGCGC AGTGCAGCCA GCGGGCATGA CCGATCCACC 
AGGGGCGCCG CCGCCGGCGC CTGCAAGCCG CGGGTGAAGA AGAAAGTCCG GCCCCGCTCT 
TCTCAGAGCG AGAAGGTAGG GAGCAGCAGC AGGGAGTTGA CTAGAAGTAA GAAAGTGACC 
CGTAGCAAGA ACGTGACCGG GACCCAGGTA GAGGAGGTGA CCAAGATCGA GGAGGCGACC 
CAAACCGAGG AAGTAACTGT GGCAGAAGAG GTGACCCAGA CCGACAACAT GGCCAAGACC 
GAGGAGATGG TCCAGACGGA GGAAATGGAA ACACCCAGAC TCAGCGTGAT CGTCACCCAC 
AGCAATGAGA GGTATGACCT TCTTGTTACC CCACAGCAAG GTAACAGTGA GCCAGTTGTC 
CAAGACTTGG CTCAGCTTGT TGAAGAGGCC ACAGGAGTTC CACTACCTTT TCAGAAGCTC 
ATATTTAAGG GAAAATCTCT GAAAGAAATG GAAACACCGT TGTCAGCACT TGGAATGCAA 
AATGGTTGCC GAGTCATGTT AATTGGTGAA AAGAGCAATC CAGAAGAAGA GGTTGAGTTA 
AAGAAGCTGA AAGATTTGGA GGTATCTGCA GAGAAGATAG CTAACCACCT GCAAGAATTG 
AATAAAGAGC TTTCTGGCAT CCAGCAGGGT TTTCTGGCTA AGGAATTGCA AGCGGAGGCT 
CTCTGCAAAC TTGATAGGAA AGTAAAAGCA ACAATTGAGC AATTCATGAA GATCTTGGAG 
GAGATTGACA CAATGGTCCT ACCAGAACAA TTTAAAGACA GCAGGCTAAA AAGGAAGAAT 
TTGGTGAAAA AGGTTCAGGT GTTCTTAGCA GAGTGTGACA CAGTGGAGCA ATACATCTGC 
CAAGAGACAG AGCGGCTGCA GTCTACAAAC TTGGCCCTGG CTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BAG-1L Protects SH-SY5Y Neuroblastoma Cells Against Hypoxia/Re-oxygenation Through Up-Regulating HSP70 and Activating PI3K/AKT Signaling Pathway.
Neurochemical research42(10)2861-2868(2017 Oct)
Wang Y,Jia C,Li QS,Xie CY,Zhang N,Qu Y

BAG-1M co-activates BACE1 transcription through NF-?B and accelerates A? production and memory deficit in Alzheimer's disease mouse model.
Biochimica et biophysica acta1863(9)2398-2407(2017 Sep)
Shi Z,Hong Y,Zhang K,Wang J,Zheng L,Zhang Z,Hu Z,Han X,Han Y,Chen T,Yao Q,Cui H,Hong W

Regulation of osteoblast development by Bcl-2-associated athanogene-1 (BAG-1).
Scientific reports633504(2016 Sep)
Greenhough J,Papadakis ES,Cutress RI,Townsend PA,Oreffo RO,Tare RS

The BAG-1 isoform BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells during activation of inflammatory signaling.
Journal of cell science127(Pt 16)3568-77(2014 Aug)
Mashukova A,Kozhekbaeva Z,Forteza R,Dulam V,Figueroa Y,Warren R,Salas PJ

Characterization of interactions between the anti-apoptotic protein BAG-1 and Hsc70 molecular chaperones.
The Journal of biological chemistry273(35)22506-14(1998 Aug)
Stuart JK,Myszka DG,Joss L,Mitchell RS,McDonald SM,Xie Z,Takayama S,Reed JC,Ely KR

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Bag1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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