Atpif1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atpif1
Entrez Gene ID	11983
Full Name	ATPase inhibitory factor 1
Synonyms	Atpi,IF(1),If1
General protein information	Preferred Names ATPase inhibitory factor 1 Names ATPase inhibitor, mitochondrial inhibitor of F(1)F(o)-ATPase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.3
Summary	This gene encodes a member of the ATPase inhibitor family of proteins. This protein has been shown to negatively regulate the ATP hydrolysis activity of the F1Fo-ATPase. Knockdown of this gene is associated with reduced heme synthesis in differentiating erythroid cells. Misregulation of this gene has been found to lead to increased aerobic glycolysis in mouse cancer cells, while high expression levels of this gene have been correlated with gastric and liver cancer severity in human patients. A pseudogene of this gene has been identified. [provided by RefSeq, Apr 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_030253106.1	XP_030108966.1	ATPase inhibitor, mitochondrial isoform X1
XM_030253107.1	XP_030108967.1	ATPase inhibitor, mitochondrial isoform X1
XM_006538502.1	XP_006538565.1	ATPase inhibitor, mitochondrial isoform X1
NM_007512.4	NP_031538.2	ATPase inhibitor, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ATPIF1	XP_003339032.1
Danio rerio (zebrafish)	atpif1a	NP_001082990.1
Rattus norvegicus (Norway rat)	Atpif1	NP_037047.2
	ATPIF1	XP_001113002.1
Canis lupus familiaris (dog)	ATPIF1	XP_865710.1
Homo sapiens (human)	ATPIF1	NP_057395.1
Bos taurus (cattle)	ATPIF1	NP_787010.1
Mus musculus (house mouse)	Atpif1	NP_031538.2
Danio rerio (zebrafish)	atpif1b	NP_001038324.1
Xenopus tropicalis (tropical clawed frog)	atpif1	NP_001135565.1

Gene Ontology
Bibliography

Related articles in PubMed

Knockout of the ATPase inhibitory factor 1 protects the heart from pressure overload-induced cardiac hypertrophy.
Yang K, Long Q, Saja K, Huang F, Pogwizd SM, Zhou L, Yoshida M, Yang Q
Scientific reports7(1)10501(2017 Sep)

Down-regulation of oxidative phosphorylation in the liver by expression of the ATPase inhibitory factor 1 induces a tumor-promoter metabolic state.
Santacatterina F, S?nchez-Cenizo L, Formentini L, Mobasher MA, Casas E, Rueda CB, Mart?nez-Reyes I, N??ez de Arenas C, Garc?a-Berm?dez J, Zapata JM, S?nchez-Arag? M, Satr?stegui J, Valverde ?M, Cuezva JM
Oncotarget7(1)490-508(2016 Jan)

PKA Phosphorylates the ATPase Inhibitory Factor 1 and Inactivates Its Capacity to Bind and Inhibit the Mitochondrial H(+)-ATP Synthase.
Garc?a-Berm?dez J, S?nchez-Arag? M, Soldevilla B, Del Arco A, Nuevo-Tapioles C, Cuezva JM
Cell reports12(12)2143-55(2015 Sep)

ATPase inhibitory factor 1 is a prognostic marker and contributes to proliferation and invasion of human gastric cancer cells.
Yin T, Lu L, Xiong Z, Wei S, Cui D
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie7090-6(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that oxidative phosphorylation (OXPHOS) is inhibited in the liver of mice expressing human ATPase inhibitory factor 1 (hIF1).
Title: Down-regulation of oxidative phosphorylation in the liver by expression of the ATPase inhibitory factor 1 induces a tumor-promoter metabolic state.

PKA phosphorylates the ATPase inhibitory factor 1 and inactivates its capacity to bind and inhibit the mitochondrial H(+)-ATP synthase.
Title: PKA Phosphorylates the ATPase Inhibitory Factor 1 and Inactivates Its Capacity to Bind and Inhibit the Mitochondrial H(+)-ATP Synthase.

induces VEGF expression and promotes xenograft tumor-induced angiogenesis
Title: Reciprocal activation between ATPase inhibitory factor 1 and NF-?B drives hepatocellular carcinoma angiogenesis and metastasis.

IF1 is not an essential protein for mice despite its ubiquitous presence in eukaryotes.
Title: IF1, a natural inhibitor of mitochondrial ATP synthase, is not essential for the normal growth and breeding of mice.

methionine deprivation results in an antioxidant response that includes an increase in the levels of HSPA1A and DNAJB1 mRNA
Title: The serum- and glucocorticoid-induced protein kinase-1 (Sgk-1) mitochondria connection: identification of the IF-1 inhibitor of the F(1)F(0)-ATPase as a mitochondria-specific binding target and the stress-induced mitochondrial localization of endogenous Sgk-1.

The following Atpif1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atpif1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15293	NM_007512.4 Latest version!	Mus musculus ATPase inhibitory factor 1 (Atpif1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu15293	XM_006538502.1 Latest version!	Mus musculus ATPase inhibitory factor 1 (Atpif1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu15293	XM_030253107.1 Latest version!	Mus musculus ATPase inhibitory factor 1 (Atpif1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu15293	XM_030253106.1 Latest version!	Mus musculus ATPase inhibitory factor 1 (Atpif1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atpif1 ( NM_007512.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031538.2 Mus musculus ATPase inhibitory factor 1 (Atpif1), mRNA.

Price & Availability↑

CloneID	OMu15293
Clone ID Related Accession (Same CDS sequence)	XM_030253106.1 , XM_030253107.1 , XM_006538502.1 , NM_007512.4
Accession Version	NM_007512.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	ATPase inhibitor, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK152389.1 and BU055998.1. On Apr 3, 2015 this sequence version replaced NM_007512.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGCT CGGCGTTGGC AGTTCGGGCT CGGTTCGGTG TCTGGGGTAT GAAGGTCCTG 
CAAACCCGAG GCTTCGTCTC GGACTCGTCG GATAGCATGG ATACGGGCGC TGGCTCCATC 
CGAGAAGCTG GTGGAGCCTT CGGAAAACGA GAAAAGGCTG AAGAGGATCG GTACTTCCGA 
GAGAAGACTA AAGAACAGCT GGCTGCCCTG AGGAAACACC ATGAAGATGA GATTGACCAC 
CATTCGAAGG AGATAGAGCG TCTGCAGAAG CAAATTGAAC GCCATAAGAA GAAGATCCAA 
CAACTAAAGA ATAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031538.2
CDS	1044..1364
Translation	MAGSALAVRARFGVWGMKVLQTRGFVSDSSDSMDTGAGSIREAGGAFGKREKAEEDRYFREKTKEQLAALRKHHEDEIDHHSKEIERLQKQIERHKKKIQQLKNNH

Target ORF information:

RefSeq Version	NM_007512.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase inhibitory factor 1 (Atpif1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007512.4

ORF Insert Sequence:

ATGGCAGGCT CGGCGTTGGC AGTTCGGGCT CGGTTCGGTG TCTGGGGTAT GAAGGTCCTG 
CAAACCCGAG GCTTCGTCTC GGACTCGTCG GATAGCATGG ATACGGGCGC TGGCTCCATC 
CGAGAAGCTG GTGGAGCCTT CGGAAAACGA GAAAAGGCTG AAGAGGATCG GTACTTCCGA 
GAGAAGACTA AAGAACAGCT GGCTGCCCTG AGGAAACACC ATGAAGATGA GATTGACCAC 
CATTCGAAGG AGATAGAGCG TCTGCAGAAG CAAATTGAAC GCCATAAGAA GAAGATCCAA 
CAACTAAAGA ATAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atpif1 ( XM_030253106.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108966.1 Mus musculus ATPase inhibitory factor 1 (Atpif1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15293
Clone ID Related Accession (Same CDS sequence)	XM_030253106.1 , XM_030253107.1 , XM_006538502.1 , NM_007512.4
Accession Version	XM_030253106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATPase inhibitor, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCT CGGCGTTGGC AGTTCGGGCT CGGTTCGGTG TCTGGGGTAT GAAGGTCCTG 
CAAACCCGAG GCTTCGTCTC GGACTCGTCG GATAGCATGG ATACGGGCGC TGGCTCCATC 
CGAGAAGCTG GTGGAGCCTT CGGAAAACGA GAAAAGGCTG AAGAGGATCG GTACTTCCGA 
GAGAAGACTA AAGAACAGCT GGCTGCCCTG AGGAAACACC ATGAAGATGA GATTGACCAC 
CATTCGAAGG AGATAGAGCG TCTGCAGAAG CAAATTGAAC GCCATAAGAA GAAGATCCAA 
CAACTAAAGA ATAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108966.1
CDS	762..1082
Translation	MAGSALAVRARFGVWGMKVLQTRGFVSDSSDSMDTGAGSIREAGGAFGKREKAEEDRYFREKTKEQLAALRKHHEDEIDHHSKEIERLQKQIERHKKKIQQLKNNH

Target ORF information:

RefSeq Version	XM_030253106.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase inhibitory factor 1 (Atpif1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253106.1

ORF Insert Sequence:

ATGGCAGGCT CGGCGTTGGC AGTTCGGGCT CGGTTCGGTG TCTGGGGTAT GAAGGTCCTG 
CAAACCCGAG GCTTCGTCTC GGACTCGTCG GATAGCATGG ATACGGGCGC TGGCTCCATC 
CGAGAAGCTG GTGGAGCCTT CGGAAAACGA GAAAAGGCTG AAGAGGATCG GTACTTCCGA 
GAGAAGACTA AAGAACAGCT GGCTGCCCTG AGGAAACACC ATGAAGATGA GATTGACCAC 
CATTCGAAGG AGATAGAGCG TCTGCAGAAG CAAATTGAAC GCCATAAGAA GAAGATCCAA 
CAACTAAAGA ATAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atpif1 ( XM_030253107.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108967.1 Mus musculus ATPase inhibitory factor 1 (Atpif1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu15293
Clone ID Related Accession (Same CDS sequence)	XM_030253106.1 , XM_030253107.1 , XM_006538502.1 , NM_007512.4
Accession Version	XM_030253107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATPase inhibitor, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCT CGGCGTTGGC AGTTCGGGCT CGGTTCGGTG TCTGGGGTAT GAAGGTCCTG 
CAAACCCGAG GCTTCGTCTC GGACTCGTCG GATAGCATGG ATACGGGCGC TGGCTCCATC 
CGAGAAGCTG GTGGAGCCTT CGGAAAACGA GAAAAGGCTG AAGAGGATCG GTACTTCCGA 
GAGAAGACTA AAGAACAGCT GGCTGCCCTG AGGAAACACC ATGAAGATGA GATTGACCAC 
CATTCGAAGG AGATAGAGCG TCTGCAGAAG CAAATTGAAC GCCATAAGAA GAAGATCCAA 
CAACTAAAGA ATAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108967.1
CDS	595..915
Translation	MAGSALAVRARFGVWGMKVLQTRGFVSDSSDSMDTGAGSIREAGGAFGKREKAEEDRYFREKTKEQLAALRKHHEDEIDHHSKEIERLQKQIERHKKKIQQLKNNH

Target ORF information:

RefSeq Version	XM_030253107.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase inhibitory factor 1 (Atpif1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253107.1

ORF Insert Sequence:

ATGGCAGGCT CGGCGTTGGC AGTTCGGGCT CGGTTCGGTG TCTGGGGTAT GAAGGTCCTG 
CAAACCCGAG GCTTCGTCTC GGACTCGTCG GATAGCATGG ATACGGGCGC TGGCTCCATC 
CGAGAAGCTG GTGGAGCCTT CGGAAAACGA GAAAAGGCTG AAGAGGATCG GTACTTCCGA 
GAGAAGACTA AAGAACAGCT GGCTGCCCTG AGGAAACACC ATGAAGATGA GATTGACCAC 
CATTCGAAGG AGATAGAGCG TCTGCAGAAG CAAATTGAAC GCCATAAGAA GAAGATCCAA 
CAACTAAAGA ATAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atpif1 ( XM_006538502.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538565.1 Mus musculus ATPase inhibitory factor 1 (Atpif1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15293
Clone ID Related Accession (Same CDS sequence)	XM_030253106.1 , XM_030253107.1 , XM_006538502.1 , NM_007512.4
Accession Version	XM_006538502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	ATPase inhibitor, mitochondrial isoform X1
Comment

ATGGCAGGCT CGGCGTTGGC AGTTCGGGCT CGGTTCGGTG TCTGGGGTAT GAAGGTCCTG 
CAAACCCGAG GCTTCGTCTC GGACTCGTCG GATAGCATGG ATACGGGCGC TGGCTCCATC 
CGAGAAGCTG GTGGAGCCTT CGGAAAACGA GAAAAGGCTG AAGAGGATCG GTACTTCCGA 
GAGAAGACTA AAGAACAGCT GGCTGCCCTG AGGAAACACC ATGAAGATGA GATTGACCAC 
CATTCGAAGG AGATAGAGCG TCTGCAGAAG CAAATTGAAC GCCATAAGAA GAAGATCCAA 
CAACTAAAGA ATAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538565.1
Translation	MAGSALAVRARFGVWGMKVLQTRGFVSDSSDSMDTGAGSIREAGGAFGKREKAEEDRYFREKTKEQLAALRKHHEDEIDHHSKEIERLQKQIERHKKKIQQLKNNH

Target ORF information:

RefSeq Version	XM_006538502.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus ATPase inhibitory factor 1 (Atpif1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538502.1

ORF Insert Sequence:

ATGGCAGGCT CGGCGTTGGC AGTTCGGGCT CGGTTCGGTG TCTGGGGTAT GAAGGTCCTG 
CAAACCCGAG GCTTCGTCTC GGACTCGTCG GATAGCATGG ATACGGGCGC TGGCTCCATC 
CGAGAAGCTG GTGGAGCCTT CGGAAAACGA GAAAAGGCTG AAGAGGATCG GTACTTCCGA 
GAGAAGACTA AAGAACAGCT GGCTGCCCTG AGGAAACACC ATGAAGATGA GATTGACCAC 
CATTCGAAGG AGATAGAGCG TCTGCAGAAG CAAATTGAAC GCCATAAGAA GAAGATCCAA 
CAACTAAAGA ATAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Knockout of the ATPase inhibitory factor 1 protects the heart from pressure overload-induced cardiac hypertrophy.
Scientific reports7(1)10501(2017 Sep)
Yang K,Long Q,Saja K,Huang F,Pogwizd SM,Zhou L,Yoshida M,Yang Q

Down-regulation of oxidative phosphorylation in the liver by expression of the ATPase inhibitory factor 1 induces a tumor-promoter metabolic state.
Oncotarget7(1)490-508(2016 Jan)
Santacatterina F,S?nchez-Cenizo L,Formentini L,Mobasher MA,Casas E,Rueda CB,Mart?nez-Reyes I,N??ez de Arenas C,Garc?a-Berm?dez J,Zapata JM,S?nchez-Arag? M,Satr?stegui J,Valverde ?M,Cuezva JM

PKA Phosphorylates the ATPase Inhibitory Factor 1 and Inactivates Its Capacity to Bind and Inhibit the Mitochondrial H(+)-ATP Synthase.
Cell reports12(12)2143-55(2015 Sep)
Garc?a-Berm?dez J,S?nchez-Arag? M,Soldevilla B,Del Arco A,Nuevo-Tapioles C,Cuezva JM

ATPase inhibitory factor 1 is a prognostic marker and contributes to proliferation and invasion of human gastric cancer cells.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie7090-6(2015 Mar)
Yin T,Lu L,Xiong Z,Wei S,Cui D

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Atpif1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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