Atp6v1e1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp6v1e1
Entrez Gene ID	11973
Full Name	ATPase, H+ transporting, lysosomal V1 subunit E1
Synonyms	2410029D23Rik,Atp6e,Atp6e2,Atp6v1e,D6Ertd385e,E2,P31,Vma4
General protein information	Preferred Names ATPase, H+ transporting, lysosomal V1 subunit E1 Names V-type proton ATPase subunit E 1 ATPase, H+ transporting lysosomal (vacuolar proton pump), 32 kDa ATPase, H+ transporting, V1 subunit E ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E H(+)-ATPase E-like protein H+ ATPase subunit E V-ATPase 31 kDa subunit V-ATPase subunit E 1 VATPase, H+ transporting, lysosomal V1 subunit E1 lysosomal 31kDa vacuolar proton pump subunit E 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 F1\|6 57.01 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007510.2	NP_031536.2	V-type proton ATPase subunit E 1
NM_007510.3	NP_031536.2	V-type proton ATPase subunit E 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP6V1E1	XP_005637441.1
Rattus norvegicus (Norway rat)	Atp6v1e1	NP_942040.1
Schizosaccharomyces pombe (fission yeast)	vma4	NP_594932.1
Bos taurus (cattle)	ATP6V1E1	NP_777235.1
Neurospora crassa	NCU07446	XP_964633.2
Arabidopsis thaliana (thale cress)	VHA-E2	NP_187468.1
Oryza sativa (rice)	Os05g0480700	NP_001055857.1
Homo sapiens (human)	ATP6V1E1	NP_001687.1
Mus musculus (house mouse)	Atp6v1e1	NP_031536.2
Gallus gallus (chicken)	ATP6V1E1	NP_001006246.1
Danio rerio (zebrafish)	atp6v1e1b	NP_775361.1
Drosophila melanogaster (fruit fly)	Vha26	NP_524237.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002401	XP_312551.1
Caenorhabditis elegans (roundworm)	vha-8	NP_501040.1
Magnaporthe oryzae (rice blast fungus)	MGG_02770	XP_003720979.1
Pan troglodytes (chimpanzee)	ATP6V1E1	XP_514965.2
Saccharomyces cerevisiae (baker's yeast)	VMA4	NP_014977.3
Kluyveromyces lactis	KLLA0F02541g	XP_455195.1
Xenopus tropicalis (tropical clawed frog)	atp6v1e1	NP_989123.1

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative Trait Locus Mapping of Macrophage Cholesterol Metabolism and CRISPR/Cas9 Editing Implicate an ACAT1 Truncation as a Causal Modifier Variant.
Hai Q, Ritchey B, Robinet P, Alzayed AM, Brubaker G, Zhang J, Smith JD
Arteriosclerosis, thrombosis, and vascular biology38(1)83-91(2018 Jan)

HNF1B controls epithelial organization and cell polarity during ureteric bud branching and collecting duct morphogenesis.
Desgrange A, Heliot C, Skovorodkin I, Akram SU, Heikkil? J, Ronkainen VP, Miinalainen I, Vainio SJ, Cereghini S
Development (Cambridge, England)144(24)4704-4719(2017 Dec)

Polarization of the vacuolar adenosine triphosphatase delineates a transition to high-grade pancreatic intraepithelial neoplasm lesions.
Sreekumar BK, Belinsky GS, Einwachter H, Rhim AD, Schmid R, Chung C
Pancreas43(8)1256-63(2014 Nov)

Atp6v0a4 knockout mouse is a model of distal renal tubular acidosis with hearing loss, with additional extrarenal phenotype.
Norgett EE, Golder ZJ, Lorente-C?novas B, Ingham N, Steel KP, Karet Frankl FE
Proceedings of the National Academy of Sciences of the United States of America109(34)13775-80(2012 Aug)

RAB-like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly.
Lo JC, Jamsai D, O'Connor AE, Borg C, Clark BJ, Whisstock JC, Field MC, Adams V, Ishikawa T, Aitken RJ, Whittle B, Goodnow CC, Ormandy CJ, O'Bryan MK
PLoS genetics8(10)e1002969(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In pancreatic intraepithelial lesions, V1E subunits localized to the basolateral domain in specific regions. Blocking the v-ATPase disrupted Wnt/beta-catenin signaling in primary PanIN cells.
Title: Polarization of the vacuolar adenosine triphosphatase delineates a transition to high-grade pancreatic intraepithelial neoplasm lesions.

These results suggest that subunit E, especially its amino-terminal domain, plays a pertinent role in the assembly of V-ATPase subunits in vacuolar membranes from mice and yeast.
Title: Defective assembly of a hybrid vacuolar H(+)-ATPase containing the mouse testis-specific E1 isoform and yeast subunits.

These results suggest that the B1 vacuolar H(+)-ATPase subunit is necessary for the furosemide-induced acute urinary acidification.
Title: The connecting tubule is the main site of the furosemide-induced urinary acidification by the vacuolar H+-ATPase.

The following Atp6v1e1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp6v1e1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00300	NM_007510.3 Latest version!	Mus musculus ATPase, H+ transporting, lysosomal V1 subunit E1 (Atp6v1e1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu00300	NM_007510.2	Mus musculus ATPase, H+ transporting, lysosomal V1 subunit E1 (Atp6v1e1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp6v1e1 ( NM_007510.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031536.2 Mus musculus ATPase, H+ transporting, lysosomal V1 subunit E1 (Atp6v1e1), mRNA.

Price & Availability↑

CloneID	OMu00300
Clone ID Related Accession (Same CDS sequence)	NM_007510.2 , NM_007510.3
Accession Version	NM_007510.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Mus musculus(house mouse)
Product	V-type proton ATPase subunit E 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC003421.1. On Mar 17, 2004 this sequence version replaced NM_007510.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003421.1, AB074758.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCTCA GCGATGCAGA TGTACAGAAG CAGATTAAGC ACATGATGGC TTTCATTGAA 
CAAGAAGCCA ATGAGAAAGC AGAAGAAATA GATGCAAAGG CAGAAGAAGA GTTCAACATT 
GAGAAAGGTC GCCTTGTGCA AACGCAAAGA CTGAAGATTA TGGAATACTA CGAGAAGAAA 
GAAAAGCAGA TTGAGCAGCA GAAGAAAATT CAGATGTCCA ACTTGATGAA TCAAGCAAGG 
CTCAAAGTCC TCAGAGCAAG GGATGACCTC ATCACTGATC TGCTAAATGA GGCAAAGCAG 
AGACTCAGTA AGGTGGTAAA AGATACGACC CGTTACCAAG TGCTGCTGGA TGGGCTGGTC 
CTTCAGGGCT TGTACCAGCT GCTGGAGCCT CGAATGATCG TGCGTTGCAG AAAACAAGAT 
TTCCCTTTGG TGAAGGCCGC AGTACAAAAA GCAATCCCTA TGTATAAAAT TGCTACCAAA 
AAGGATGTTG ATGTCCAGAT TGACCAGGAG GCCTACCTGC CTGAGGAAAT AGCTGGTGGC 
GTTGAGATCT ATAATGGGGA TCGCAAGATA AAGGTTTCCA ACACCCTGGA AAGCCGGCTG 
GACCTGATAG CCCAGCAGAT GATGCCAGAA GTCCGTGGAG CCTTGTTTGG TGCAAATGCC 
AATAGGAAGT TTCTGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031536.2
CDS	66..746
Translation	MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKKDVDVQIDQEAYLPEEIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD

Target ORF information:

RefSeq Version	NM_007510.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, H+ transporting, lysosomal V1 subunit E1 (Atp6v1e1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007510.2

ORF Insert Sequence:

ATGGCGCTCA GCGATGCAGA TGTACAGAAG CAGATTAAGC ACATGATGGC TTTCATTGAA 
CAAGAAGCCA ATGAGAAAGC AGAAGAAATA GATGCAAAGG CAGAAGAAGA GTTCAACATT 
GAGAAAGGTC GCCTTGTGCA AACGCAAAGA CTGAAGATTA TGGAATACTA CGAGAAGAAA 
GAAAAGCAGA TTGAGCAGCA GAAGAAAATT CAGATGTCCA ACTTGATGAA TCAAGCAAGG 
CTCAAAGTCC TCAGAGCAAG GGATGACCTC ATCACTGATC TGCTAAATGA GGCAAAGCAG 
AGACTCAGTA AGGTGGTAAA AGATACGACC CGTTACCAAG TGCTGCTGGA TGGGCTGGTC 
CTTCAGGGCT TGTACCAGCT GCTGGAGCCT CGAATGATCG TGCGTTGCAG AAAACAAGAT 
TTCCCTTTGG TGAAGGCCGC AGTACAAAAA GCAATCCCTA TGTATAAAAT TGCTACCAAA 
AAGGATGTTG ATGTCCAGAT TGACCAGGAG GCCTACCTGC CTGAGGAAAT AGCTGGTGGC 
GTTGAGATCT ATAATGGGGA TCGCAAGATA AAGGTTTCCA ACACCCTGGA AAGCCGGCTG 
GACCTGATAG CCCAGCAGAT GATGCCAGAA GTCCGTGGAG CCTTGTTTGG TGCAAATGCC 
AATAGGAAGT TTCTGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp6v1e1 ( NM_007510.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031536.2 Mus musculus ATPase, H+ transporting, lysosomal V1 subunit E1 (Atp6v1e1), mRNA.

Price & Availability↑

CloneID	OMu00300
Clone ID Related Accession (Same CDS sequence)	NM_007510.2 , NM_007510.3
Accession Version	NM_007510.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	V-type proton ATPase subunit E 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK139791.1 and AI844873.1. On Feb 14, 2018 this sequence version replaced NM_007510.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003421.1, AB074758.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCTCA GCGATGCAGA TGTACAGAAG CAGATTAAGC ACATGATGGC TTTCATTGAA 
CAAGAAGCCA ATGAGAAAGC AGAAGAAATA GATGCAAAGG CAGAAGAAGA GTTCAACATT 
GAGAAAGGTC GCCTTGTGCA AACGCAAAGA CTGAAGATTA TGGAATACTA CGAGAAGAAA 
GAAAAGCAGA TTGAGCAGCA GAAGAAAATT CAGATGTCCA ACTTGATGAA TCAAGCAAGG 
CTCAAAGTCC TCAGAGCAAG GGATGACCTC ATCACTGATC TGCTAAATGA GGCAAAGCAG 
AGACTCAGTA AGGTGGTAAA AGATACGACC CGTTACCAAG TGCTGCTGGA TGGGCTGGTC 
CTTCAGGGCT TGTACCAGCT GCTGGAGCCT CGAATGATCG TGCGTTGCAG AAAACAAGAT 
TTCCCTTTGG TGAAGGCCGC AGTACAAAAA GCAATCCCTA TGTATAAAAT TGCTACCAAA 
AAGGATGTTG ATGTCCAGAT TGACCAGGAG GCCTACCTGC CTGAGGAAAT AGCTGGTGGC 
GTTGAGATCT ATAATGGGGA TCGCAAGATA AAGGTTTCCA ACACCCTGGA AAGCCGGCTG 
GACCTGATAG CCCAGCAGAT GATGCCAGAA GTCCGTGGAG CCTTGTTTGG TGCAAATGCC 
AATAGGAAGT TTCTGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031536.2
CDS	79..759
Translation	MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKKDVDVQIDQEAYLPEEIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD

Target ORF information:

RefSeq Version	NM_007510.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, H+ transporting, lysosomal V1 subunit E1 (Atp6v1e1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007510.3

ORF Insert Sequence:

ATGGCGCTCA GCGATGCAGA TGTACAGAAG CAGATTAAGC ACATGATGGC TTTCATTGAA 
CAAGAAGCCA ATGAGAAAGC AGAAGAAATA GATGCAAAGG CAGAAGAAGA GTTCAACATT 
GAGAAAGGTC GCCTTGTGCA AACGCAAAGA CTGAAGATTA TGGAATACTA CGAGAAGAAA 
GAAAAGCAGA TTGAGCAGCA GAAGAAAATT CAGATGTCCA ACTTGATGAA TCAAGCAAGG 
CTCAAAGTCC TCAGAGCAAG GGATGACCTC ATCACTGATC TGCTAAATGA GGCAAAGCAG 
AGACTCAGTA AGGTGGTAAA AGATACGACC CGTTACCAAG TGCTGCTGGA TGGGCTGGTC 
CTTCAGGGCT TGTACCAGCT GCTGGAGCCT CGAATGATCG TGCGTTGCAG AAAACAAGAT 
TTCCCTTTGG TGAAGGCCGC AGTACAAAAA GCAATCCCTA TGTATAAAAT TGCTACCAAA 
AAGGATGTTG ATGTCCAGAT TGACCAGGAG GCCTACCTGC CTGAGGAAAT AGCTGGTGGC 
GTTGAGATCT ATAATGGGGA TCGCAAGATA AAGGTTTCCA ACACCCTGGA AAGCCGGCTG 
GACCTGATAG CCCAGCAGAT GATGCCAGAA GTCCGTGGAG CCTTGTTTGG TGCAAATGCC 
AATAGGAAGT TTCTGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative Trait Locus Mapping of Macrophage Cholesterol Metabolism and CRISPR/Cas9 Editing Implicate an ACAT1 Truncation as a Causal Modifier Variant.
Arteriosclerosis, thrombosis, and vascular biology38(1)83-91(2018 Jan)
Hai Q,Ritchey B,Robinet P,Alzayed AM,Brubaker G,Zhang J,Smith JD

HNF1B controls epithelial organization and cell polarity during ureteric bud branching and collecting duct morphogenesis.
Development (Cambridge, England)144(24)4704-4719(2017 Dec)
Desgrange A,Heliot C,Skovorodkin I,Akram SU,Heikkil? J,Ronkainen VP,Miinalainen I,Vainio SJ,Cereghini S

Polarization of the vacuolar adenosine triphosphatase delineates a transition to high-grade pancreatic intraepithelial neoplasm lesions.
Pancreas43(8)1256-63(2014 Nov)
Sreekumar BK,Belinsky GS,Einwachter H,Rhim AD,Schmid R,Chung C

Atp6v0a4 knockout mouse is a model of distal renal tubular acidosis with hearing loss, with additional extrarenal phenotype.
Proceedings of the National Academy of Sciences of the United States of America109(34)13775-80(2012 Aug)
Norgett EE,Golder ZJ,Lorente-C?novas B,Ingham N,Steel KP,Karet Frankl FE

RAB-like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly.
PLoS genetics8(10)e1002969(2012)
Lo JC,Jamsai D,O'Connor AE,Borg C,Clark BJ,Whisstock JC,Field MC,Adams V,Ishikawa T,Aitken RJ,Whittle B,Goodnow CC,Ormandy CJ,O'Bryan MK

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Atp6v1e1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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