Fxyd2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fxyd2
Entrez Gene ID	11936
Full Name	FXYD domain-containing ion transport regulator 2
Synonyms	Atp1g1
General protein information	Preferred Names FXYD domain-containing ion transport regulator 2 Names sodium/potassium-transporting ATPase subunit gamma ATPase, Na+/K+ transporting, gamma 1 polypeptide Na(+)/K(+) ATPase subunit gamma sodium pump gamma chain sodium/potassium-transporting ATPase gamma chain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A5.2
Summary	This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The Type III integral membrane protein encoded by this gene is the gamma subunit of the Na,K-ATPase present on the plasma membrane. Although the Na,K-ATPase does not depend on the gamma subunit to be functional, it is thought that the gamma subunit modulates the enzyme's activity by inducing ion channel activity. Multiple transcript variants have been described for this gene that are expressed in tissue-specific and developmental stage-specific patterns and encode proteins that differ at the N-terminus. [provided by RefSeq, Sep 2009].

mRNA and Protein(s)

mRNA	Protein	Name
XM_030244032.1	XP_030099892.1	sodium/potassium-transporting ATPase subunit gamma isoform X1
NM_007503.3	NP_031529.2	sodium/potassium-transporting ATPase subunit gamma isoform a
NM_052823.2	NP_439888.1	sodium/potassium-transporting ATPase subunit gamma isoform b

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Gurdziel K, Vogt KR, Walton KD, Schneider GK, Gumucio DL
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)

A dystonia-like movement disorder with brain and spinal neuronal defects is caused by mutation of the mouse laminin ?1 subunit, Lamb1.
Liu YB, Tewari A, Salameh J, Arystarkhova E, Hampton TG, Brashear A, Ozelius LJ, Khodakhah K, Sweadner KJ
eLife4(2015 Dec)

FXYD2, a ? subunit of Na?, K?-ATPase, maintains persistent mechanical allodynia induced by inflammation.
Wang F, Cai B, Li KC, Hu XY, Lu YJ, Wang Q, Bao L, Zhang X
Cell research25(3)318-34(2015 Mar)

Hyperplasia of pancreatic beta cells and improved glucose tolerance in mice deficient in the FXYD2 subunit of Na,K-ATPase.
Arystarkhova E, Liu YB, Salazar C, Stanojevic V, Clifford RJ, Kaplan JH, Kidder GM, Sweadner KJ
The Journal of biological chemistry288(10)7077-85(2013 Mar)

Regulation of the Na,K-ATPase gamma-subunit FXYD2 by Runx1 and Ret signaling in normal and injured non-peptidergic nociceptive sensory neurons.
Vent?o S, Bourane S, M?chaly I, Sar C, Abdel Samad O, Puech S, Blostein R, Valmier J, Pattyn A, Carroll P
PloS one7(1)e29852(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FXYD2 activity may be a fundamental mechanism underlying the persistent hypersensitivity to pain induced by inflammation.
Title: FXYD2, a ? subunit of Na?, K?-ATPase, maintains persistent mechanical allodynia induced by inflammation.

Data suggest that in pancreatic beta cells regulatory subunit of Na,K-ATPase FXYD2 plays a role in Akt signaling pathways associated with cell growth and proliferation.
Title: Hyperplasia of pancreatic beta cells and improved glucose tolerance in mice deficient in the FXYD2 subunit of Na,K-ATPase.

The transcription factor Runx1 controls FXYD2 expression during the maturation of the somato-sensory system, partly through regulation of the tyrosine kinase receptor Ret.
Title: Regulation of the Na,K-ATPase gamma-subunit FXYD2 by Runx1 and Ret signaling in normal and injured non-peptidergic nociceptive sensory neurons.

direct and critical role of the gamma-subunit on IMCD3 cell survival and/or adaptation in response to ionic hypertonic stress
Title: Silencing and overexpression of the gamma-subunit of Na-K-ATPase directly affect survival of IMCD3 cells in response to hypertonic stress.

FXYD2 has a role in Na+ affinity and regulating thermal stability in mice
Title: Na,K-ATPase from mice lacking the gamma subunit (FXYD2) exhibits altered Na+ affinity and decreased thermal stability.

The following Fxyd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fxyd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14977	NM_007503.3 Latest version!	Mus musculus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu15147	NM_052823.2 Latest version!	Mus musculus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu15147	XM_030244032.1 Latest version!	Mus musculus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fxyd2 ( NM_007503.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031529.2 Mus musculus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant a, mRNA.

Price & Availability↑

CloneID	OMu14977
Clone ID Related Accession (Same CDS sequence)	NM_007503.3
Accession Version	NM_007503.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	sodium/potassium-transporting ATPase subunit gamma isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK002228.1 and AI316264.1. On Jan 18, 2007 this sequence version replaced NM_007503.2. Transcript Variant: This variant (a) represents the most frequently occurring transcript and it encodes the longest protein (isoform a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC024614.1, CB601762.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGGG AAATATCAGA TCTGTCAGCG AACAGTGGTG GCAGTGCCAA GGGGACAGAG 
AATCCCTTCG AGTACGACTA TGAAACCGTC CGCAAAGGAG GCCTGATCTT CGCGGGCCTG 
GCCTTCGTCG TGGGCCTCCT CATCATTCTC AGCAAAAGGT TCCGCTGTGG GGGCGGTAAG 
AAACATAGGC AGGTCAATGA AGATGAACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031529.2
CDS	54..266
Translation	MAGEISDLSANSGGSAKGTENPFEYDYETVRKGGLIFAGLAFVVGLLIILSKRFRCGGGKKHRQVNEDEL

Target ORF information:

RefSeq Version	NM_007503.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007503.3

ORF Insert Sequence:

ATGGCTGGGG AAATATCAGA TCTGTCAGCG AACAGTGGTG GCAGTGCCAA GGGGACAGAG 
AATCCCTTCG AGTACGACTA TGAAACCGTC CGCAAAGGAG GCCTGATCTT CGCGGGCCTG 
GCCTTCGTCG TGGGCCTCCT CATCATTCTC AGCAAAAGGT TCCGCTGTGG GGGCGGTAAG 
AAACATAGGC AGGTCAATGA AGATGAACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fxyd2 ( NM_052823.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_439888.1 Mus musculus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant b, mRNA.

Price & Availability↑

CloneID	OMu15147
Clone ID Related Accession (Same CDS sequence)	XM_030244032.1 , NM_052823.2
Accession Version	NM_052823.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Mus musculus(house mouse)
Product	sodium/potassium-transporting ATPase subunit gamma isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC162176.2 and CB600439.1. On Jan 18, 2007 this sequence version replaced NM_052823.1. Transcript Variant: This variant (b) uses an alternate segment for its 5' UTR and coding region, compared to variant a. The resulting protein (isoform b) has a shorter and distinct N-terminus when it is compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY626244.1, BG870050.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACAGGT GGTACCTGGG TGGCAGTGCC AAGGGGACAG AGAATCCCTT CGAGTACGAC 
TATGAAACCG TCCGCAAAGG AGGCCTGATC TTCGCGGGCC TGGCCTTCGT CGTGGGCCTC 
CTCATCATTC TCAGCAAAAG GTTCCGCTGT GGGGGCGGTA AGAAACATAG GCAGGTCAAT 
GAAGATGAAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_439888.1
CDS	145..339
Translation	MDRWYLGGSAKGTENPFEYDYETVRKGGLIFAGLAFVVGLLIILSKRFRCGGGKKHRQVNEDEL

Target ORF information:

RefSeq Version	NM_052823.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052823.2

ORF Insert Sequence:

ATGGACAGGT GGTACCTGGG TGGCAGTGCC AAGGGGACAG AGAATCCCTT CGAGTACGAC 
TATGAAACCG TCCGCAAAGG AGGCCTGATC TTCGCGGGCC TGGCCTTCGT CGTGGGCCTC 
CTCATCATTC TCAGCAAAAG GTTCCGCTGT GGGGGCGGTA AGAAACATAG GCAGGTCAAT 
GAAGATGAAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fxyd2 ( XM_030244032.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099892.1 Mus musculus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15147
Clone ID Related Accession (Same CDS sequence)	XM_030244032.1 , NM_052823.2
Accession Version	XM_030244032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sodium/potassium-transporting ATPase subunit gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGT GGTACCTGGG TGGCAGTGCC AAGGGGACAG AGAATCCCTT CGAGTACGAC 
TATGAAACCG TCCGCAAAGG AGGCCTGATC TTCGCGGGCC TGGCCTTCGT CGTGGGCCTC 
CTCATCATTC TCAGCAAAAG GTTCCGCTGT GGGGGCGGTA AGAAACATAG GCAGGTCAAT 
GAAGATGAAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099892.1
CDS	609..803
Translation	MDRWYLGGSAKGTENPFEYDYETVRKGGLIFAGLAFVVGLLIILSKRFRCGGGKKHRQVNEDEL

Target ORF information:

RefSeq Version	XM_030244032.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus FXYD domain-containing ion transport regulator 2 (Fxyd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244032.1

ORF Insert Sequence:

ATGGACAGGT GGTACCTGGG TGGCAGTGCC AAGGGGACAG AGAATCCCTT CGAGTACGAC 
TATGAAACCG TCCGCAAAGG AGGCCTGATC TTCGCGGGCC TGGCCTTCGT CGTGGGCCTC 
CTCATCATTC TCAGCAAAAG GTTCCGCTGT GGGGGCGGTA AGAAACATAG GCAGGTCAAT 
GAAGATGAAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)
Gurdziel K,Vogt KR,Walton KD,Schneider GK,Gumucio DL

A dystonia-like movement disorder with brain and spinal neuronal defects is caused by mutation of the mouse laminin ?1 subunit, Lamb1.
eLife4(2015 Dec)
Liu YB,Tewari A,Salameh J,Arystarkhova E,Hampton TG,Brashear A,Ozelius LJ,Khodakhah K,Sweadner KJ

FXYD2, a ? subunit of Na?, K?-ATPase, maintains persistent mechanical allodynia induced by inflammation.
Cell research25(3)318-34(2015 Mar)
Wang F,Cai B,Li KC,Hu XY,Lu YJ,Wang Q,Bao L,Zhang X

Hyperplasia of pancreatic beta cells and improved glucose tolerance in mice deficient in the FXYD2 subunit of Na,K-ATPase.
The Journal of biological chemistry288(10)7077-85(2013 Mar)
Arystarkhova E,Liu YB,Salazar C,Stanojevic V,Clifford RJ,Kaplan JH,Kidder GM,Sweadner KJ

Regulation of the Na,K-ATPase gamma-subunit FXYD2 by Runx1 and Ret signaling in normal and injured non-peptidergic nociceptive sensory neurons.
PloS one7(1)e29852(2012)
Vent?o S,Bourane S,M?chaly I,Sar C,Abdel Samad O,Puech S,Blostein R,Valmier J,Pattyn A,Carroll P

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Fxyd2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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