Apod cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apod
Entrez Gene ID	11815
Full Name	apolipoprotein D
General protein information	Preferred Names apolipoprotein D Names apolipoprotein D
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 B2\|16 21.41 cM
Summary	The protein encoded by this gene is a component of high-density lipoprotein (HDL), but is unique in that it shares greater structural similarity to lipocalin than to other members of the apolipoprotein family, and has a wider tissue expression pattern. The encoded protein is involved in lipid metabolism, and ablation of this gene results in defects in triglyceride metabolism. Elevated levels of this gene product have been observed in multiple tissues of Niemann-Pick disease mouse models, as well as in some tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007470.4	NP_031496.2	apolipoprotein D precursor
NM_001301353.1	NP_001288282.1	apolipoprotein D precursor
NM_001301354.1	NP_001288283.1	apolipoprotein D precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	zgc:123339	NP_001032784.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009281	XP_320076.4
Canis lupus familiaris (dog)	APOD	XP_535780.1
Bos taurus (cattle)	APOD	NP_001069769.1
Pan troglodytes (chimpanzee)	APOD	XP_516965.1
	LOC709223	XP_001098104.2
Mus musculus (house mouse)	Apod	NP_031496.2
Homo sapiens (human)	APOD	NP_001638.1
Rattus norvegicus (Norway rat)	Apod	NP_036909.1
Gallus gallus (chicken)	APOD	NP_001011692.1
Danio rerio (zebrafish)	apod	NP_001070050.1
Drosophila melanogaster (fruit fly)	NLaz	NP_001259867.1

Gene Ontology
Bibliography

Related articles in PubMed

Myelin extracellular leaflet compaction requires apolipoprotein D membrane management to optimize lysosomal-dependent recycling and glycocalyx removal.
Garc?a-Mateo N, Pascua-Maestro R, P?rez-Castellanos A, Lillo C, Sanchez D, Ganfornina MD
Glia66(3)670-687(2018 Mar)

Phosphatidylserine Ameliorates Neurodegenerative Symptoms and Enhances Axonal Transport in a Mouse Model of Familial Dysautonomia.
Naftelberg S, Abramovitch Z, Gluska S, Yannai S, Joshi Y, Donyo M, Ben-Yaakov K, Gradus T, Zonszain J, Farhy C, Ashery-Padan R, Perlson E, Ast G
PLoS genetics12(12)e1006486(2016 Dec)

Genetic deficiency of apolipoprotein D in the mouse is associated with nonfasting hypertriglyceridemia and hyperinsulinemia.
Jim?nez-Palomares M, C?zar-Castellano I, Ganfornina MD, S?nchez D, Perdomo G
Metabolism: clinical and experimental60(12)1767-74(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

apoD protein levels are variable across different brain regions.
Title: Cerebral Apolipoprotein-D Is Hypoglycosylated Compared to Peripheral Tissues and Is Variably Expressed in Mouse and Human Brain Regions.

It regulates amyloid plaque pathology in a mouse model of Alzheimer's disease
Title: Apolipoprotein D modulates amyloid pathology in APP/PS1 Alzheimer's disease mice.

apoD may be part of an intrinsic cardioprotective system, possibly as a consequence of its antioxidation activity.
Title: Identification of apolipoprotein D as a cardioprotective gene using a mouse model of lethal atherosclerotic coronary artery disease.

Changes in hypothalamus of ApoD(-/-) mice may indicate potential role of ApoD in regulation of endocrine functions of somatostatin in a receptor-dependent manner.
Title: Immunohistochemical distribution of somatostatin and somatostatin receptor subtypes (SSTR1-5) in hypothalamus of ApoD knockout mice brain.

Abeta25-35 induces neuroprotective ApoD expression in hippocampal cells in response to stress-induced growth arrest.
Title: Amyloid-?25-35 induces apolipoprotein D Synthesis and growth arrest in HT22 hippocampal cells.

The following Apod gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apod cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20443	NM_001301354.1 Latest version!	Mus musculus apolipoprotein D (Apod), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu20443	NM_001301353.1 Latest version!	Mus musculus apolipoprotein D (Apod), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu20443	NM_007470.4 Latest version!	Mus musculus apolipoprotein D (Apod), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apod ( NM_007470.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031496.2 Mus musculus apolipoprotein D (Apod), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20443
Clone ID Related Accession (Same CDS sequence)	NM_007470.4 , NM_001301353.1 , NM_001301354.1
Accession Version	NM_007470.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	apolipoprotein D precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC130815.4, AK135046.1, AK162392.1 and BY596441.1. On Aug 13, 2014 this sequence version replaced NM_007470.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK162392.1, AK135046.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGACCA TGCTGATGTT CCTGGCCACG CTGGCGGGTC TCTTCACCAC AGCCAAAGGA 
CAAAATTTCC ATCTTGGGAA ATGCCCGTCT CCTCCTGTGC AAGAGAATTT TGACGTGAAA 
AAGTATCTTG GAAGATGGTA CGAAATTGAG AAGATCCCAG CGAGCTTTGA GAAAGGAAAC 
TGCATTCAAG CCAACTACTC GCTGATGGAG AACGGAAACA TCGAAGTGCT AAACAAGGAG 
CTGAGTCCTG ATGGAACCAT GAACCAAGTA AAGGGTGAAG CCAAACAGAG CAACGTCTCA 
GAGCCAGCCA AGCTGGAAGT CCAGTTCTTC CCGTTGATGC CACCGGCACC CTACTGGATC 
CTGGCCACCG ATTATGAAAA CTATGCCCTC GTGTACTCCT GCACCACCTT CTTCTGGCTC 
TTCCATGTGG ATTTTGTTTG GATTCTTGGA AGAAATCCTT ATCTCCCTCC AGAAACAATA 
ACCTACCTAA AAGATATCCT TACTTCTAAT GGCATCGACA TCGAAAAAAT GACAACAACA 
GATCAAGCGA ACTGCCCGGA CTTCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031496.2
CDS	356..925
Translation	MVTMLMFLATLAGLFTTAKGQNFHLGKCPSPPVQENFDVKKYLGRWYEIEKIPASFEKGNCIQANYSLMENGNIEVLNKELSPDGTMNQVKGEAKQSNVSEPAKLEVQFFPLMPPAPYWILATDYENYALVYSCTTFFWLFHVDFVWILGRNPYLPPETITYLKDILTSNGIDIEKMTTTDQANCPDFL

Target ORF information:

RefSeq Version	NM_007470.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus apolipoprotein D (Apod), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007470.4

ORF Insert Sequence:

ATGGTGACCA TGCTGATGTT CCTGGCCACG CTGGCGGGTC TCTTCACCAC AGCCAAAGGA 
CAAAATTTCC ATCTTGGGAA ATGCCCGTCT CCTCCTGTGC AAGAGAATTT TGACGTGAAA 
AAGTATCTTG GAAGATGGTA CGAAATTGAG AAGATCCCAG CGAGCTTTGA GAAAGGAAAC 
TGCATTCAAG CCAACTACTC GCTGATGGAG AACGGAAACA TCGAAGTGCT AAACAAGGAG 
CTGAGTCCTG ATGGAACCAT GAACCAAGTA AAGGGTGAAG CCAAACAGAG CAACGTCTCA 
GAGCCAGCCA AGCTGGAAGT CCAGTTCTTC CCGTTGATGC CACCGGCACC CTACTGGATC 
CTGGCCACCG ATTATGAAAA CTATGCCCTC GTGTACTCCT GCACCACCTT CTTCTGGCTC 
TTCCATGTGG ATTTTGTTTG GATTCTTGGA AGAAATCCTT ATCTCCCTCC AGAAACAATA 
ACCTACCTAA AAGATATCCT TACTTCTAAT GGCATCGACA TCGAAAAAAT GACAACAACA 
GATCAAGCGA ACTGCCCGGA CTTCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apod ( NM_001301353.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288282.1 Mus musculus apolipoprotein D (Apod), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20443
Clone ID Related Accession (Same CDS sequence)	NM_007470.4 , NM_001301353.1 , NM_001301354.1
Accession Version	NM_001301353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	apolipoprotein D precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC130815.4, AK158118.1, AK162392.1 and BY596441.1. On Aug 13, 2014 this sequence version replaced XM_006521717.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK158118.1, DV054128.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGACCA TGCTGATGTT CCTGGCCACG CTGGCGGGTC TCTTCACCAC AGCCAAAGGA 
CAAAATTTCC ATCTTGGGAA ATGCCCGTCT CCTCCTGTGC AAGAGAATTT TGACGTGAAA 
AAGTATCTTG GAAGATGGTA CGAAATTGAG AAGATCCCAG CGAGCTTTGA GAAAGGAAAC 
TGCATTCAAG CCAACTACTC GCTGATGGAG AACGGAAACA TCGAAGTGCT AAACAAGGAG 
CTGAGTCCTG ATGGAACCAT GAACCAAGTA AAGGGTGAAG CCAAACAGAG CAACGTCTCA 
GAGCCAGCCA AGCTGGAAGT CCAGTTCTTC CCGTTGATGC CACCGGCACC CTACTGGATC 
CTGGCCACCG ATTATGAAAA CTATGCCCTC GTGTACTCCT GCACCACCTT CTTCTGGCTC 
TTCCATGTGG ATTTTGTTTG GATTCTTGGA AGAAATCCTT ATCTCCCTCC AGAAACAATA 
ACCTACCTAA AAGATATCCT TACTTCTAAT GGCATCGACA TCGAAAAAAT GACAACAACA 
GATCAAGCGA ACTGCCCGGA CTTCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288282.1
CDS	352..921
Translation	MVTMLMFLATLAGLFTTAKGQNFHLGKCPSPPVQENFDVKKYLGRWYEIEKIPASFEKGNCIQANYSLMENGNIEVLNKELSPDGTMNQVKGEAKQSNVSEPAKLEVQFFPLMPPAPYWILATDYENYALVYSCTTFFWLFHVDFVWILGRNPYLPPETITYLKDILTSNGIDIEKMTTTDQANCPDFL

Target ORF information:

RefSeq Version	NM_001301353.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus apolipoprotein D (Apod), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301353.1

ORF Insert Sequence:

ATGGTGACCA TGCTGATGTT CCTGGCCACG CTGGCGGGTC TCTTCACCAC AGCCAAAGGA 
CAAAATTTCC ATCTTGGGAA ATGCCCGTCT CCTCCTGTGC AAGAGAATTT TGACGTGAAA 
AAGTATCTTG GAAGATGGTA CGAAATTGAG AAGATCCCAG CGAGCTTTGA GAAAGGAAAC 
TGCATTCAAG CCAACTACTC GCTGATGGAG AACGGAAACA TCGAAGTGCT AAACAAGGAG 
CTGAGTCCTG ATGGAACCAT GAACCAAGTA AAGGGTGAAG CCAAACAGAG CAACGTCTCA 
GAGCCAGCCA AGCTGGAAGT CCAGTTCTTC CCGTTGATGC CACCGGCACC CTACTGGATC 
CTGGCCACCG ATTATGAAAA CTATGCCCTC GTGTACTCCT GCACCACCTT CTTCTGGCTC 
TTCCATGTGG ATTTTGTTTG GATTCTTGGA AGAAATCCTT ATCTCCCTCC AGAAACAATA 
ACCTACCTAA AAGATATCCT TACTTCTAAT GGCATCGACA TCGAAAAAAT GACAACAACA 
GATCAAGCGA ACTGCCCGGA CTTCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apod ( NM_001301354.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288283.1 Mus musculus apolipoprotein D (Apod), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu20443
Clone ID Related Accession (Same CDS sequence)	NM_007470.4 , NM_001301353.1 , NM_001301354.1
Accession Version	NM_001301354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	apolipoprotein D precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC130815.4, AK160729.1, AK162392.1 and BY596441.1. On Aug 13, 2014 this sequence version replaced XM_006521716.1. Transcript Variant: This variant (3) lacks an exon in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK160729.1, BG175079.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGACCA TGCTGATGTT CCTGGCCACG CTGGCGGGTC TCTTCACCAC AGCCAAAGGA 
CAAAATTTCC ATCTTGGGAA ATGCCCGTCT CCTCCTGTGC AAGAGAATTT TGACGTGAAA 
AAGTATCTTG GAAGATGGTA CGAAATTGAG AAGATCCCAG CGAGCTTTGA GAAAGGAAAC 
TGCATTCAAG CCAACTACTC GCTGATGGAG AACGGAAACA TCGAAGTGCT AAACAAGGAG 
CTGAGTCCTG ATGGAACCAT GAACCAAGTA AAGGGTGAAG CCAAACAGAG CAACGTCTCA 
GAGCCAGCCA AGCTGGAAGT CCAGTTCTTC CCGTTGATGC CACCGGCACC CTACTGGATC 
CTGGCCACCG ATTATGAAAA CTATGCCCTC GTGTACTCCT GCACCACCTT CTTCTGGCTC 
TTCCATGTGG ATTTTGTTTG GATTCTTGGA AGAAATCCTT ATCTCCCTCC AGAAACAATA 
ACCTACCTAA AAGATATCCT TACTTCTAAT GGCATCGACA TCGAAAAAAT GACAACAACA 
GATCAAGCGA ACTGCCCGGA CTTCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288283.1
CDS	286..855
Translation	MVTMLMFLATLAGLFTTAKGQNFHLGKCPSPPVQENFDVKKYLGRWYEIEKIPASFEKGNCIQANYSLMENGNIEVLNKELSPDGTMNQVKGEAKQSNVSEPAKLEVQFFPLMPPAPYWILATDYENYALVYSCTTFFWLFHVDFVWILGRNPYLPPETITYLKDILTSNGIDIEKMTTTDQANCPDFL

Target ORF information:

RefSeq Version	NM_001301354.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus apolipoprotein D (Apod), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301354.1

ORF Insert Sequence:

ATGGTGACCA TGCTGATGTT CCTGGCCACG CTGGCGGGTC TCTTCACCAC AGCCAAAGGA 
CAAAATTTCC ATCTTGGGAA ATGCCCGTCT CCTCCTGTGC AAGAGAATTT TGACGTGAAA 
AAGTATCTTG GAAGATGGTA CGAAATTGAG AAGATCCCAG CGAGCTTTGA GAAAGGAAAC 
TGCATTCAAG CCAACTACTC GCTGATGGAG AACGGAAACA TCGAAGTGCT AAACAAGGAG 
CTGAGTCCTG ATGGAACCAT GAACCAAGTA AAGGGTGAAG CCAAACAGAG CAACGTCTCA 
GAGCCAGCCA AGCTGGAAGT CCAGTTCTTC CCGTTGATGC CACCGGCACC CTACTGGATC 
CTGGCCACCG ATTATGAAAA CTATGCCCTC GTGTACTCCT GCACCACCTT CTTCTGGCTC 
TTCCATGTGG ATTTTGTTTG GATTCTTGGA AGAAATCCTT ATCTCCCTCC AGAAACAATA 
ACCTACCTAA AAGATATCCT TACTTCTAAT GGCATCGACA TCGAAAAAAT GACAACAACA 
GATCAAGCGA ACTGCCCGGA CTTCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Myelin extracellular leaflet compaction requires apolipoprotein D membrane management to optimize lysosomal-dependent recycling and glycocalyx removal.
Glia66(3)670-687(2018 Mar)
Garc?a-Mateo N,Pascua-Maestro R,P?rez-Castellanos A,Lillo C,Sanchez D,Ganfornina MD

Phosphatidylserine Ameliorates Neurodegenerative Symptoms and Enhances Axonal Transport in a Mouse Model of Familial Dysautonomia.
PLoS genetics12(12)e1006486(2016 Dec)
Naftelberg S,Abramovitch Z,Gluska S,Yannai S,Joshi Y,Donyo M,Ben-Yaakov K,Gradus T,Zonszain J,Farhy C,Ashery-Padan R,Perlson E,Ast G

Genetic deficiency of apolipoprotein D in the mouse is associated with nonfasting hypertriglyceridemia and hyperinsulinemia.
Metabolism: clinical and experimental60(12)1767-74(2011 Dec)
Jim?nez-Palomares M,C?zar-Castellano I,Ganfornina MD,S?nchez D,Perdomo G

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Apod cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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