Apoc3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apoc3
Entrez Gene ID	11814
Full Name	apolipoprotein C-III
Synonyms	apo-CIII,apoC-III
General protein information	Preferred Names apolipoprotein C-III Names apolipoprotein C-III apolipoprotein C3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 A5.2\|9 25.36 cM
Summary	This gene encodes an apolipoprotein which is the major protein component of very-low-density lipoproteins (VLDL) and a minor component of high-density lipoproteins (HDL). The encoded protein is thought to regulate the metabolism of triglyceride-rich lipoproteins and play a role in lipid storage and the mobilization of fat cells. This gene is clustered with three other apolipoprotein genes on chromosome 9 and is associated with coronary disease. Mice lacking this gene have lower levels of total cholesterol in the plasma. Mutations in the human genes causes hyperalphalipoproteinemia 2, a disorder of lipid metabolism which results in a favorable lipid profile (lower LDL-cholesterol, higher HDL-cholesterol and lower levels of serum triglycerides when fasting and after a meal). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001289833.1	NP_001276762.1	apolipoprotein C-III isoform c
NM_001289755.1	NP_001276684.1	apolipoprotein C-III isoform a
NM_023114.4	NP_075603.1	apolipoprotein C-III isoform b precursor
NM_001289756.1	NP_001276685.1	apolipoprotein C-III isoform b precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Apoc3	NP_001257982.1
Mus musculus (house mouse)	Apoc3	NP_075603.1

Gene Ontology
Bibliography

Related articles in PubMed

The apolipoprotein C-III (Gln38Lys) variant associated with human hypertriglyceridemia is a gain-of-function mutation.
Sundaram M, Curtis KR, Amir Alipour M, LeBlond ND, Margison KD, Yaworski RA, Parks RJ, McIntyre AD, Hegele RA, Fullerton MD, Yao Z
Journal of lipid research58(11)2188-2196(2017 Nov)

Key differences between apoC-III regulation and expression in intestine and liver.
West G, Rodia C, Li D, Johnson Z, Dong H, Kohan AB
Biochemical and biophysical research communications491(3)747-753(2017 09)

A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels.
Khetarpal SA, Zeng X, Millar JS, Vitali C, Somasundara AVH, Zanoni P, Landro JA, Barucci N, Zavadoski WJ, Sun Z, de Haard H, Toth IV, Peloso GM, Natarajan P, Cuchel M, Lund-Katz S, Phillips MC, Tall AR, Kathiresan S, DaSilva-Jardine P, Yates NA, Rader DJ
Nature medicine23(9)1086-1094(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data strongly suggest that intestinal apoC-III is not a FoxO1 target and support the idea that apoC-III is not regulated coordinately with hepatic apoC-III, and establishes another key aspect of apoC-III that is unique in the intestine from the liver.
Title: Key differences between apoC-III regulation and expression in intestine and liver.

intestinal apoC-III overexpression results in the secretion of smaller, less dense chylomicron particles along with reduced triacylglycerol secretion from the intestine
Title: Using primary murine intestinal enteroids to study dietary TAG absorption, lipoprotein synthesis, and the role of apoC-III in the intestine.

APOC3, whose dysregulation is liable for hypertriglyceridemia, is not a predisposing factor for linking overnutrition to NAFLD in obesity
Title: APOC3 Protein Is Not a Predisposing Factor for Fat-induced Nonalcoholic Fatty Liver Disease in Mice.

ApoC-III inhibits turnover of TG-rich lipoproteins primarily through a hepatic clearance mechanism mediated by the LDLR/LRP1 axis
Title: ApoC-III inhibits clearance of triglyceride-rich lipoproteins through LDL family receptors.

Severe hypertriglyceridaemia resulting from ApoCIII overexpression promotes restenosis and atherosclerosis
Title: Aggravated restenosis and atherogenesis in ApoCIII transgenic mice but lack of protection in ApoCIII knockouts: the effect of authentic triglyceride-rich lipoproteins with and without ApoCIII.

The following Apoc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apoc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00021	NM_001289833.1 Latest version!	Mus musculus apolipoprotein C-III (Apoc3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu13770	NM_001289755.1 Latest version!	Mus musculus apolipoprotein C-III (Apoc3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu13748	NM_001289756.1 Latest version!	Mus musculus apolipoprotein C-III (Apoc3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu13748	NM_023114.4 Latest version!	Mus musculus apolipoprotein C-III (Apoc3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apoc3 ( NM_001289833.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276762.1 Mus musculus apolipoprotein C-III (Apoc3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu00021
Clone ID Related Accession (Same CDS sequence)	NM_001289833.1
Accession Version	NM_001289833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-07
Organism	Mus musculus(house mouse)
Product	apolipoprotein C-III isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116503.17. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (c) has a shorter N-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AI553221.1, AA623288.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACAAG CCTCCAAGAC GGTCCAGGAT GCGCTAAGTA GCGTGCAGGA GTCCGATATA 
GCTGTGGTGG CCAGGGGCTG GATGGACAAT CACTTCAGAT CCCTGAAAGG CTACTGGAGC 
AAGTTTACTG ACAAGTTCAC CGGCTTCTGG GATTCTAACC CTGAGGACCA ACCAACTCCA 
GCTATTGAGT CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276762.1
CDS	92..286
Translation	MEQASKTVQDALSSVQESDIAVVARGWMDNHFRSLKGYWSKFTDKFTGFWDSNPEDQPTPAIES

Target ORF information:

RefSeq Version	NM_001289833.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus apolipoprotein C-III (Apoc3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289833.1

ORF Insert Sequence:

ATGGAACAAG CCTCCAAGAC GGTCCAGGAT GCGCTAAGTA GCGTGCAGGA GTCCGATATA 
GCTGTGGTGG CCAGGGGCTG GATGGACAAT CACTTCAGAT CCCTGAAAGG CTACTGGAGC 
AAGTTTACTG ACAAGTTCAC CGGCTTCTGG GATTCTAACC CTGAGGACCA ACCAACTCCA 
GCTATTGAGT CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apoc3 ( NM_001289755.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276684.1 Mus musculus apolipoprotein C-III (Apoc3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13770
Clone ID Related Accession (Same CDS sequence)	NM_001289755.1
Accession Version	NM_001289755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-07
Organism	Mus musculus(house mouse)
Product	apolipoprotein C-III isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116503.17. On Jan 31, 2014 this sequence version replaced XM_006509955.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB949719.1, CA456578.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGG GGAGGAGAGG AAGGAAGGGA AGAAACAAAG AGCTGGAGGG AGAAGCTCTC 
ACCACCCAGC CATCTAGCCC ACAGAAGGCT TGGGACTCAT GGTACGTAGG TGCCATGCAG 
CCCCGGACGC TCCTCACTGT GGCCCTCTTG GCTCTCCTGG CATCTGCCCG AGCTGAAGAG 
GTAGAGGGAT CCTTGCTGCT GGGCTCTGTA CAGGGCTACA TGGAACAAGC CTCCAAGACG 
GTCCAGGATG CGCTAAGTAG CGTGCAGGAG TCCGATATAG CTGTGGTGGC CAGGGGCTGG 
ATGGACAATC ACTTCAGATC CCTGAAAGGC TACTGGAGCA AGTTTACTGA CAAGTTCACC 
GGCTTCTGGG ATTCTAACCC TGAGGACCAA CCAACTCCAG CTATTGAGTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276684.1
CDS	45..458
Translation	MPLGRRGRKGRNKELEGEALTTQPSSPQKAWDSWYVGAMQPRTLLTVALLALLASARAEEVEGSLLLGSVQGYMEQASKTVQDALSSVQESDIAVVARGWMDNHFRSLKGYWSKFTDKFTGFWDSNPEDQPTPAIES

Target ORF information:

RefSeq Version	NM_001289755.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus apolipoprotein C-III (Apoc3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289755.1

ORF Insert Sequence:

ATGCCCCTGG GGAGGAGAGG AAGGAAGGGA AGAAACAAAG AGCTGGAGGG AGAAGCTCTC 
ACCACCCAGC CATCTAGCCC ACAGAAGGCT TGGGACTCAT GGTACGTAGG TGCCATGCAG 
CCCCGGACGC TCCTCACTGT GGCCCTCTTG GCTCTCCTGG CATCTGCCCG AGCTGAAGAG 
GTAGAGGGAT CCTTGCTGCT GGGCTCTGTA CAGGGCTACA TGGAACAAGC CTCCAAGACG 
GTCCAGGATG CGCTAAGTAG CGTGCAGGAG TCCGATATAG CTGTGGTGGC CAGGGGCTGG 
ATGGACAATC ACTTCAGATC CCTGAAAGGC TACTGGAGCA AGTTTACTGA CAAGTTCACC 
GGCTTCTGGG ATTCTAACCC TGAGGACCAA CCAACTCCAG CTATTGAGTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apoc3 ( NM_023114.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075603.1 Mus musculus apolipoprotein C-III (Apoc3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu13748
Clone ID Related Accession (Same CDS sequence)	NM_023114.4 , NM_001289756.1
Accession Version	NM_023114.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	apolipoprotein C-III isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK008260.1 and AC116503.17. On Jan 31, 2014 this sequence version replaced NM_023114.3. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (b) has a shorter N-terminus compared to isoform a. Variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB949799.1, AK002908.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCCC GGACGCTCCT CACTGTGGCC CTCTTGGCTC TCCTGGCATC TGCCCGAGCT 
GAAGAGGTAG AGGGATCCTT GCTGCTGGGC TCTGTACAGG GCTACATGGA ACAAGCCTCC 
AAGACGGTCC AGGATGCGCT AAGTAGCGTG CAGGAGTCCG ATATAGCTGT GGTGGCCAGG 
GGCTGGATGG ACAATCACTT CAGATCCCTG AAAGGCTACT GGAGCAAGTT TACTGACAAG 
TTCACCGGCT TCTGGGATTC TAACCCTGAG GACCAACCAA CTCCAGCTAT TGAGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075603.1
CDS	55..354
Translation	MQPRTLLTVALLALLASARAEEVEGSLLLGSVQGYMEQASKTVQDALSSVQESDIAVVARGWMDNHFRSLKGYWSKFTDKFTGFWDSNPEDQPTPAIES

Target ORF information:

RefSeq Version	NM_023114.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus apolipoprotein C-III (Apoc3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023114.4

ORF Insert Sequence:

ATGCAGCCCC GGACGCTCCT CACTGTGGCC CTCTTGGCTC TCCTGGCATC TGCCCGAGCT 
GAAGAGGTAG AGGGATCCTT GCTGCTGGGC TCTGTACAGG GCTACATGGA ACAAGCCTCC 
AAGACGGTCC AGGATGCGCT AAGTAGCGTG CAGGAGTCCG ATATAGCTGT GGTGGCCAGG 
GGCTGGATGG ACAATCACTT CAGATCCCTG AAAGGCTACT GGAGCAAGTT TACTGACAAG 
TTCACCGGCT TCTGGGATTC TAACCCTGAG GACCAACCAA CTCCAGCTAT TGAGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apoc3 ( NM_001289756.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276685.1 Mus musculus apolipoprotein C-III (Apoc3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13748
Clone ID Related Accession (Same CDS sequence)	NM_023114.4 , NM_001289756.1
Accession Version	NM_001289756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	apolipoprotein C-III isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from W61891.1, AC116503.17 and AK008260.1. On Jan 31, 2014 this sequence version replaced XM_006509954.1. Transcript Variant: This variant (2) represents the use of an alternate promoter, differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (b) has a shorter N-terminus compared to isoform a. Variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: W61891.1, W50727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCCC GGACGCTCCT CACTGTGGCC CTCTTGGCTC TCCTGGCATC TGCCCGAGCT 
GAAGAGGTAG AGGGATCCTT GCTGCTGGGC TCTGTACAGG GCTACATGGA ACAAGCCTCC 
AAGACGGTCC AGGATGCGCT AAGTAGCGTG CAGGAGTCCG ATATAGCTGT GGTGGCCAGG 
GGCTGGATGG ACAATCACTT CAGATCCCTG AAAGGCTACT GGAGCAAGTT TACTGACAAG 
TTCACCGGCT TCTGGGATTC TAACCCTGAG GACCAACCAA CTCCAGCTAT TGAGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276685.1
CDS	129..428
Translation	MQPRTLLTVALLALLASARAEEVEGSLLLGSVQGYMEQASKTVQDALSSVQESDIAVVARGWMDNHFRSLKGYWSKFTDKFTGFWDSNPEDQPTPAIES

Target ORF information:

RefSeq Version	NM_001289756.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus apolipoprotein C-III (Apoc3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289756.1

ORF Insert Sequence:

ATGCAGCCCC GGACGCTCCT CACTGTGGCC CTCTTGGCTC TCCTGGCATC TGCCCGAGCT 
GAAGAGGTAG AGGGATCCTT GCTGCTGGGC TCTGTACAGG GCTACATGGA ACAAGCCTCC 
AAGACGGTCC AGGATGCGCT AAGTAGCGTG CAGGAGTCCG ATATAGCTGT GGTGGCCAGG 
GGCTGGATGG ACAATCACTT CAGATCCCTG AAAGGCTACT GGAGCAAGTT TACTGACAAG 
TTCACCGGCT TCTGGGATTC TAACCCTGAG GACCAACCAA CTCCAGCTAT TGAGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The apolipoprotein C-III (Gln38Lys) variant associated with human hypertriglyceridemia is a gain-of-function mutation.
Journal of lipid research58(11)2188-2196(2017 Nov)
Sundaram M,Curtis KR,Amir Alipour M,LeBlond ND,Margison KD,Yaworski RA,Parks RJ,McIntyre AD,Hegele RA,Fullerton MD,Yao Z

Key differences between apoC-III regulation and expression in intestine and liver.
Biochemical and biophysical research communications491(3)747-753(2017 09)
West G,Rodia C,Li D,Johnson Z,Dong H,Kohan AB

A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels.
Nature medicine23(9)1086-1094(2017 Sep)
Khetarpal SA,Zeng X,Millar JS,Vitali C,Somasundara AVH,Zanoni P,Landro JA,Barucci N,Zavadoski WJ,Sun Z,de Haard H,Toth IV,Peloso GM,Natarajan P,Cuchel M,Lund-Katz S,Phillips MC,Tall AR,Kathiresan S,DaSilva-Jardine P,Yates NA,Rader DJ

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Apoc3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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