Apoa1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apoa1
Entrez Gene ID	11806
Full Name	apolipoprotein A-I
Synonyms	Alp-1,Apoa-1,Brp-14,Ltw-1,Lvtw-1,Sep-1,Sep-2,Sep2,apo-AI,apoA-I
General protein information	Preferred Names apolipoprotein A-I Names apolipoprotein A-I apolipoprotein A1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 A5.2\|9 25.36 cM
Summary	This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is the major protein component of plasma high density lipoprotein (HDL). This protein facilitates the removal of cholesterol and other fats from tissues by transporting them to the liver for excretion. This protein is a cofactor for lecithin cholesterolacyltransferase, an enzyme that catalyzes the conversion of free cholesterol to cholesteryl esters. Mutations in this gene in humans causes familial HDL deficiency, Tangier disease and familial visceral amyloidosis. Similar clinical features are exhibited by mice with mutations in this gene. This gene is clustered with three other apolipoprotein genes on chromosome 9. [provided by RefSeq, Dec 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009692.4	NP_033822.2	apolipoprotein A-I preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	APOA1	NP_000030.1
	APOA1	XP_001090774.1
Rattus norvegicus (Norway rat)	Apoa1	NP_036870.1
Gallus gallus (chicken)	APOA1	NP_990856.1
Danio rerio (zebrafish)	apoa1a	NP_571203.1
Canis lupus familiaris (dog)	APOA1	XP_536564.2
Danio rerio (zebrafish)	apoa1b	NP_001093614.2
Xenopus tropicalis (tropical clawed frog)	apoa1	NP_001005127.1
Pan troglodytes (chimpanzee)	APOA1	XP_001153383.1
Bos taurus (cattle)	APOA1	NP_776667.2
Mus musculus (house mouse)	Apoa1	NP_033822.2

Gene Ontology
Bibliography

Related articles in PubMed

Mapping, genetic isolation, and characterization of genetic loci that determine resistance to atherosclerosis in C3H mice.
Wang SS, Shi W, Wang X, Velky L, Greenlee S, Wang MT, Drake TA, Lusis AJ
Arteriosclerosis, thrombosis, and vascular biology27(12)2671-6(2007 Dec)

Characterization of a new mouse model for human apolipoprotein A-I/C-III/A-IV deficiency.
Mezdour H, Larigauderie G, Castro G, Torpier G, Fruchart J, Nowak M, Fruchart JC, Rouis M, Maeda N
Journal of lipid research47(5)912-20(2006 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ABCA1-derived nascent high-density lipoprotein-apolipoprotein AI and lipids metabolically segregate.
Title: ABCA1-Derived Nascent High-Density Lipoprotein-Apolipoprotein AI and Lipids Metabolically Segregate.

This study suggests that enhancement of macrophage cholesterol metabolism by PPARgammais not contributed by activating ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI, which is not involved by CD36 expression either.
Title: Activation of PPAR? does not contribute to macrophage ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI.

Preincubation of endothelial cells with apoA-I protected against the TNF-alpha-induced inhibition of HTR-8/SVneo (trophoblast) cell integration into endothelial (UtMVEC) networks. These data suggest that a healthy lipid profile may affect pregnancy outcomes by priming endothelial cells in preparation for trophoblast invasion.
Title: The protective effect of apolipoprotein in models of trophoblast invasion and preeclampsia.

Reductions in Dio1 expression reduce the expression of ApoA-I in a 3,5,3'-triiodothyronine-/thyroid hormone response element-independent manner.
Title: Type 1 Deiodinase Regulates ApoA-I Gene Expression and ApoA-I Synthesis Independent of Thyroid Hormone Signaling.

Our results assign a novel role for 4F(apoA-I mimetic peptide ) as a modulator of the TICE pathway and suggest that the anti-inflammatory functions of 4F may be a partial consequence of the codependent intestinal transport of both 4F and cholesterol.
Title: Transintestinal transport of the anti-inflammatory drug 4F and the modulation of transintestinal cholesterol efflux.

The following Apoa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apoa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23311	NM_009692.4 Latest version!	Mus musculus apolipoprotein A-I (Apoa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apoa1 ( NM_009692.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033822.2 Mus musculus apolipoprotein A-I (Apoa1), mRNA.

Price & Availability↑

CloneID	OMu23311
Clone ID Related Accession (Same CDS sequence)	NM_009692.4
Accession Version	NM_009692.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	apolipoprotein A-I preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116503.17 and AK076187.1. On Dec 5, 2013 this sequence version replaced NM_009692.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AI304198.1, AI573781.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAAGCTG TGGTGCTGGC CGTGGCTCTG GTCTTCCTGA CAGGGAGCCA GGCTTGGCAC 
GTATGGCAGC AAGATGAACC CCAGTCCCAA TGGGACAAAG TGAAGGATTT CGCTAATGTG 
TATGTGGATG CGGTCAAAGA CAGCGGCAGA GACTATGTGT CCCAGTTTGA ATCCTCCTCC 
TTGGGCCAAC AGCTGAACCT GAATCTCCTG GAAAACTGGG ACACTCTGGG TTCAACCGTT 
AGTCAGCTGC AGGAACGGCT GGGCCCATTG ACTCGGGACT TCTGGGATAA CCTGGAGAAA 
GAAACAGATT GGGTGAGACA GGAGATGAAC AAGGACCTAG AGGAAGTGAA ACAGAAGGTG 
CAGCCCTACC TGGACGAATT CCAGAAGAAA TGGAAAGAGG ATGTGGAGCT CTACCGCCAG 
AAGGTGGCGC CTCTGGGCGC CGAGCTGCAG GAGAGCGCGC GCCAGAAGCT GCAGGAGCTG 
CAAGGGAGAC TGTCCCCTGT GGCTGAGGAA TTTCGCGACC GCATGCGCAC ACACGTAGAC 
TCTCTGCGCA CACAGCTAGC GCCCCACAGC GAACAGATGC GCGAGAGCCT GGCCCAGCGC 
CTGGCTGAGC TCAAGAGCAA CCCTACCTTG AACGAGTACC ACACCAGGGC CAAAACCCAC 
CTGAAGACAC TTGGCGAGAA AGCCAGACCT GCGCTGGAGG ACCTGCGCCA TAGTCTGATG 
CCCATGCTGG AGACGCTTAA GACCCAAGTC CAGAGTGTGA TCGACAAGGC CAGCGAGACT 
CTGACTGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033822.2
CDS	127..921
Translation	MKAVVLAVALVFLTGSQAWHVWQQDEPQSQWDKVKDFANVYVDAVKDSGRDYVSQFESSSLGQQLNLNLLENWDTLGSTVSQLQERLGPLTRDFWDNLEKETDWVRQEMNKDLEEVKQKVQPYLDEFQKKWKEDVELYRQKVAPLGAELQESARQKLQELQGRLSPVAEEFRDRMRTHVDSLRTQLAPHSEQMRESLAQRLAELKSNPTLNEYHTRAKTHLKTLGEKARPALEDLRHSLMPMLETLKTQVQSVIDKASETLTAQ

Target ORF information:

RefSeq Version	NM_009692.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus apolipoprotein A-I (Apoa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009692.4

ORF Insert Sequence:

ATGAAAGCTG TGGTGCTGGC CGTGGCTCTG GTCTTCCTGA CAGGGAGCCA GGCTTGGCAC 
GTATGGCAGC AAGATGAACC CCAGTCCCAA TGGGACAAAG TGAAGGATTT CGCTAATGTG 
TATGTGGATG CGGTCAAAGA CAGCGGCAGA GACTATGTGT CCCAGTTTGA ATCCTCCTCC 
TTGGGCCAAC AGCTGAACCT GAATCTCCTG GAAAACTGGG ACACTCTGGG TTCAACCGTT 
AGTCAGCTGC AGGAACGGCT GGGCCCATTG ACTCGGGACT TCTGGGATAA CCTGGAGAAA 
GAAACAGATT GGGTGAGACA GGAGATGAAC AAGGACCTAG AGGAAGTGAA ACAGAAGGTG 
CAGCCCTACC TGGACGAATT CCAGAAGAAA TGGAAAGAGG ATGTGGAGCT CTACCGCCAG 
AAGGTGGCGC CTCTGGGCGC CGAGCTGCAG GAGAGCGCGC GCCAGAAGCT GCAGGAGCTG 
CAAGGGAGAC TGTCCCCTGT GGCTGAGGAA TTTCGCGACC GCATGCGCAC ACACGTAGAC 
TCTCTGCGCA CACAGCTAGC GCCCCACAGC GAACAGATGC GCGAGAGCCT GGCCCAGCGC 
CTGGCTGAGC TCAAGAGCAA CCCTACCTTG AACGAGTACC ACACCAGGGC CAAAACCCAC 
CTGAAGACAC TTGGCGAGAA AGCCAGACCT GCGCTGGAGG ACCTGCGCCA TAGTCTGATG 
CCCATGCTGG AGACGCTTAA GACCCAAGTC CAGAGTGTGA TCGACAAGGC CAGCGAGACT 
CTGACTGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mapping, genetic isolation, and characterization of genetic loci that determine resistance to atherosclerosis in C3H mice.
Arteriosclerosis, thrombosis, and vascular biology27(12)2671-6(2007 Dec)
Wang SS,Shi W,Wang X,Velky L,Greenlee S,Wang MT,Drake TA,Lusis AJ

Characterization of a new mouse model for human apolipoprotein A-I/C-III/A-IV deficiency.
Journal of lipid research47(5)912-20(2006 May)
Mezdour H,Larigauderie G,Castro G,Torpier G,Fruchart J,Nowak M,Fruchart JC,Rouis M,Maeda N

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Apoa1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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