Ap3b2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ap3b2
Entrez Gene ID	11775
Full Name	adaptor-related protein complex 3, beta 2 subunit
Synonyms	AI549966,AU042881,Naptb,[b]-NAP,beta-NAP,beta3B
General protein information	Preferred Names adaptor-related protein complex 3, beta 2 subunit Names AP-3 complex subunit beta-2 AP-3 complex beta-2 subunit adapter-related protein complex 3 subunit beta-2 adaptor protein complex AP-3 beta-2 subunit adaptor protein complex AP-3 subunit beta-2 adaptor-related protein complex 3 subunit beta-2 adaptor-related protein complex AP-3, beta 2 subunit beta-3B-adaptin beta3B-adaptin clathrin assembly protein complex 3 beta-2 large chain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 D3\|7 45.71 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006540571.3	XP_006540634.1	AP-3 complex subunit beta-2 isoform X1
NM_021492.3	NP_067467.2	AP-3 complex subunit beta-2
XM_006540571.4	XP_006540634.1	AP-3 complex subunit beta-2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AP3B2	XP_005618447.1
Bos taurus (cattle)	AP3B2	NP_001230264.1
Mus musculus (house mouse)	Ap3b2	NP_067467.2
Xenopus tropicalis (tropical clawed frog)	ap3b2	NP_001121507.1
Pan troglodytes (chimpanzee)	AP3B2	XP_003314885.1
Magnaporthe oryzae (rice blast fungus)	MGG_01846	XP_003714866.1
Homo sapiens (human)	AP3B2	NP_001265441.1
Gallus gallus (chicken)	AP3B2	XP_004943691.1
Neurospora crassa	NCU06569	XP_962276.2
	AP3B2	XP_002804969.1
Rattus norvegicus (Norway rat)	Ap3b2	XP_003748939.1
Danio rerio (zebrafish)	ap3b2	XP_005163028.1

Gene Ontology
Bibliography

Related articles in PubMed

Sporadic Hirschsprung Disease: Mutational Spectrum and Novel Candidate Genes Revealed by Next-generation Sequencing.
Zhang Z, Li Q, Diao M, Liu N, Cheng W, Xiao P, Zou J, Su L, Yu K, Wu J, Li L, Jiang Q
Scientific reports7(1)14796(2017 Nov)

Exome sequencing reveals pathogenic mutations in 91 strains of mice with Mendelian disorders.
Fairfield H, Srivastava A, Ananda G, Liu R, Kircher M, Lakshminarayana A, Harris BS, Karst SY, Dionne LA, Kane CC, Curtain M, Berry ML, Ward-Bailey PF, Greenstein I, Byers C, Czechanski A, Sharp J, Palmer K, Gudis P, Martin W, Tadenev A, Bogdanik L, Pratt CH, Chang B, Schroeder DG, Cox GA, Cliften P, Milbrandt J, Murray S, Burgess R, Bergstrom DE, Donahue LR, Hamamy H, Masri A, Santoni FA, Makrythanasis P, Antonarakis SE, Shendure J, Reinholdt LG
Genome research25(7)948-57(2015 Jul)

Protein-4.1G-Mediated Membrane Trafficking Is Essential for Correct Rod Synaptic Location in the Retina and for Normal Visual Function.
Sanuki R, Watanabe S, Sugita Y, Irie S, Kozuka T, Shimada M, Ueno S, Usukura J, Furukawa T
Cell reports(2015 Feb)

Vesicles derived via AP-3-dependent recycling contribute to asynchronous release and influence information transfer.
Evstratova A, Chamberland S, Faundez V, T?th K
Nature communications55530(2014 Nov)

The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse.
Larimore J, Tornieri K, Ryder PV, Gokhale A, Zlatic SA, Craige B, Lee JD, Talbot K, Pare JF, Smith Y, Faundez V
Molecular biology of the cell22(24)4854-67(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In AP-3b2 KO mice asynchronous neurotransmitter release is attenuated and the activity-dependent increase in the precision of action potential timing is compromised.
Title: Vesicles derived via AP-3-dependent recycling contribute to asynchronous release and influence information transfer.

The following Ap3b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ap3b2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu38642	XM_006540571.4 Latest version!	Mus musculus adaptor-related protein complex 3, beta 2 subunit (Ap3b2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu12411	NM_021492.3 Latest version!	Mus musculus adaptor-related protein complex 3, beta 2 subunit (Ap3b2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$636.30 $909.00
View Old Accession Versions >>
OMu38642	XM_006540571.3	Mus musculus adaptor-related protein complex 3, beta 2 subunit (Ap3b2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ap3b2 ( XM_006540571.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540634.1 Mus musculus adaptor-related protein complex 3, beta 2 subunit (Ap3b2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38642
Clone ID Related Accession (Same CDS sequence)	XM_006540571.3 , XM_006540571.4
Accession Version	XM_006540571.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AP-3 complex subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540571.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCG CTCCGGCCTA CAGCGAAGAC AAGGGCGGCT CAGCCGGTCC CGGGGAGCCA 
GAATATGGCC ACGACCCGGC GAGCGGCGGT ATCTTCTCCT CGGACTACAA GCGGCATGAT 
GACCTGAAAG AGATGTTGGA CACCAACAAG GACTCCCTCA AGCTGGAGGC CATGAAGAGG 
ATTGTGGCAA TGATCGCCCG GGGAAAGAAT GCCTCGGACC TGTTCCCTGC AGTGGTGAAG 
AATGTGGCCT GTAAGAACAT AGAGGTGAAG AAGCTTGTCT ACGTGTATCT GGTCCGATAT 
GCCGAGGAGC AGCAAGACCT CGCTCTACTG TCCATCTCTA CTTTCCAACG TGGCCTAAAG 
GATCCCAACC AGCTGATCCG TGCCAGTGCG CTCCGGGTTC TCTCTAGCAT CCGTGTGCCC 
ATCATAGTGC CCATCATGAT GCTAGCCATC AAGGAAGCTG CCTCGGACAT GTCACCCTAC 
GTTCGGAAAA CAGCTGCTCA TGCCATCCCC AAACTCTACA GTTTGGACTC CGATCAGAAG 
GACCAGCTGA TAGAAGTCAT TGAGAAGCTT TTGGCAGATA AGACCACGCT GGTGGCAGGC 
AGTGTAGTGA TGGCCTTTGA AGAGGTATGT CCAGAACGTA TTGACCTGAT TCACAAAAAC 
TACCGGAAGC TCTGCAACCT GCTCATTGAT GTGGAGGAGT GGGGCCAGGT GGTGATTATC 
AGCATGCTCA CGCGTTATGC GCGAACGCAA TTCCTGAGCC CTACGCAGAA TGAGTCCTTG 
CTGGAGGAGA ATCCCGAGAA GGCCTTCTAT GGCTCCGAGG AGGACGAGGC CAAAGGCCCT 
GGATCAGAGG AGGCAGCCAC TGCGGCGCTG CCCGCTCGGA AGCCCTATGT AATGGACCCA 
GATCACCGGC TGCTGCTGCG GAACACCAAG CCCCTGCTGC AGAGCCGCAG CGCCGCGGTG 
GTCATGGCGG TGGCGCAGCT TTACTTCCAC CTAGCGCCTA AGGCAGAGGT GGGCGTCATC 
GCCAAGGCGC TGGTACGCCT GCTGCGCAGC CACAGTGAGG TGCAGTACGT AGTGCTCCAG 
AACGTGGCCA CCATGTCCAT CAAGCGCAGG GGTATGTTTG AACCCTACCT GAAGAGCTTC 
TACATTAGGT CCACGGATCC TACCCAGATC AAGATCCTGA AGCTGGAAGT GCTGACCAAC 
CTGGCCAATG AGACTAACAT CCCCACTGTT CTCCGGGAAT TCCAGACCTA CATTCGCAGC 
ATGGACAAAG ACTTTGTGGC AGCCACGATC CAGGCCATTG GACGATGTGC AACGAACATA 
GGCCGAGTCC GTGACACCTG TCTCAATGGC TTGGTGCAGC TTCTGTCCAA CCGTGACGAG 
CTTGTGGTTG CAGAGTCAGT GGTGGTCATT AAGAAGCTGC TGCAAATGCA GCCAGCGCAG 
CACGGTGAGA TCATCAAACA CTTGGCAAAG CTCACAGACA ACATCCAGGT GCCCATGGCA 
CGAGCCAGCA TCCTCTGGCT CATTGGAGAG TACTGTGAGC ATGTCCCCAA GATCGCACCT 
GATGTCCTGA GAAAAATGGC CAAGTCCTTC ACAGCAGAGG AGGACATCGT GAAGCTGCAG 
GTCATCAACC TGGCAGCCAA GCTGTACCTG ACTAACTCAA AGCAGACCAA GCTCTTGACC 
CAGTATGTGC TGAGTCTGGC CAAGTACGAC CAGAACTATG ATATCCGGGA TCGAGCCCGC 
TTCACCCGGC AGCTCATTGT TCCCTCTGAG CAGGGTGGTG CCCTCAGTCG CCATGCCAAG 
AAACTCTTCC TGGCCCCTAA GCCAGCCCCT ATCTTGGAGT CATCCTTCAA AGATCGTGAC 
CATTTCCAGC TGGGCTCACT GTCACACCTG CTCAATGCAA AGGCTACAGG CTACCAGGAG 
CTCCCGGACT GGCCAGAGGA AGCCCCAGAT CCATCTGTGC GCAATGTGGA GGAAGAAGAC 
CTCTCCCTTA TTGAGACCCA CGTGGGCCTG CTGGGCGAAT ACACTGAGGT ACCTGAATGG 
ACCAAATGTT CAAATCGGGA GAAGAGGAAG GAGAAGGAAA AGCCTTTCTA TTCTGACTCC 
GAGGGGGAGT CTGGCCCCAC TGAGTCTGCA GACAGCGAGC CTGAATCTGA GAGTGAGTCA 
GAGAGTAAGA GCAGCAGTGG GAGTGGCTCC GGGGAGTCTA GCAGTGAGTC GGACAATGAA 
GAAGAGGATG AGGAGAAAGG CGGAGGCAGC GAGAGTGAAC AGAGTGAGGA GGAAGATGAG 
AAGAAGAAGA AGACAAAGAA GAAGAAGGCA TCAGAGGGAC ACAGAGAAGG CTCATCATCA 
GAAGAGGGCA GTGACTCCAG CAGCAGCTCA GAGTCTGAGG TGACGTCAGA GTCGGAGGAG 
GAACAGGTGG AACCTGCCTC CTGGAGGAAG AAAACGCCTC CAGGCAGCAA GAGTGCCCCT 
GTAGCCAAGG AGATTTCCCT GCTGGACCTA GAGGATTTCA CCCCTCCTAG TGTCCAGCCT 
GTGTCTCCTC CCATGGTTGT GTCTACCAGT CTGGCTGCTG ACCTGGAAGG GCTGACACTC 
ACAGACTCCT CTCTGGTGCC TTCGCTGCTG AGTCCAGTGT CCAGCATTGG GAGGCAGGAG 
CTGCTGCACC GGGTAGCTGG AGAGGGGCTG TCTGTGGACT ATGCCTTCAG CCGCCAGCCT 
TTCTCTGGGG ATCCCCACAT GGTGTCTCTG CATATCTACT TCTCCAACAA CTCGGAGACT 
CCCATCAAGG GGCTGCATGT GGGCACTCCC AAGCTGCCTG CTGGCATCAG CATTCAAGAA 
TTCCCTGAAA TCGAGTCCCT GGCACCTGGA GAATCCACCA CTACTGTGAT GGGCATTAAC 
TTCTGCGACT CAACCCAGGC AGCCAACTTC CAGCTGTGCA CCCAAACCCG ACAGTTCTAT 
GTCTCCATTC AGCCACCTGT CGGGGAGCTG ATGGCCCCTG TATTTATGAG TGAGAATGAG 
TTCAAGAAGG AACAGGGAAA GCTGACAGGC ATGAATGAGA TCACAGAGAA GCTTACATTG 
CCAGACACCT GTCGAAGTGA TCACATGGTG GTGCAGAAAG TGACTGCCAC CGCCAACCTA 
GGTCGTGTCC CATGTGGGAC ATCTGATGAG TACAGGTTTG CAGGGAGGAC ATTAACTAGT 
GGGAGTCTGG TCCTGCTGAC TCTGGATGCC CGGGCAGCTG GAGCCGCCCA GCTGACCGTC 
AACAGTGAGA AGATGGTGAT TGGCACCATG CTGGTGAAGG ATGTGATTCA GGCTCTGACC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540634.1
CDS	378..3683
Translation	MSAAPAYSEDKGGSAGPGEPEYGHDPASGGIFSSDYKRHDDLKEMLDTNKDSLKLEAMKRIVAMIARGKNASDLFPAVVKNVACKNIEVKKLVYVYLVRYAEEQQDLALLSISTFQRGLKDPNQLIRASALRVLSSIRVPIIVPIMMLAIKEAASDMSPYVRKTAAHAIPKLYSLDSDQKDQLIEVIEKLLADKTTLVAGSVVMAFEEVCPERIDLIHKNYRKLCNLLIDVEEWGQVVIISMLTRYARTQFLSPTQNESLLEENPEKAFYGSEEDEAKGPGSEEAATAALPARKPYVMDPDHRLLLRNTKPLLQSRSAAVVMAVAQLYFHLAPKAEVGVIAKALVRLLRSHSEVQYVVLQNVATMSIKRRGMFEPYLKSFYIRSTDPTQIKILKLEVLTNLANETNIPTVLREFQTYIRSMDKDFVAATIQAIGRCATNIGRVRDTCLNGLVQLLSNRDELVVAESVVVIKKLLQMQPAQHGEIIKHLAKLTDNIQVPMARASILWLIGEYCEHVPKIAPDVLRKMAKSFTAEEDIVKLQVINLAAKLYLTNSKQTKLLTQYVLSLAKYDQNYDIRDRARFTRQLIVPSEQGGALSRHAKKLFLAPKPAPILESSFKDRDHFQLGSLSHLLNAKATGYQELPDWPEEAPDPSVRNVEEEDLSLIETHVGLLGEYTEVPEWTKCSNREKRKEKEKPFYSDSEGESGPTESADSEPESESESESKSSSGSGSGESSSESDNEEEDEEKGGGSESEQSEEEDEKKKKTKKKKASEGHREGSSSEEGSDSSSSSESEVTSESEEEQVEPASWRKKTPPGSKSAPVAKEISLLDLEDFTPPSVQPVSPPMVVSTSLAADLEGLTLTDSSLVPSLLSPVSSIGRQELLHRVAGEGLSVDYAFSRQPFSGDPHMVSLHIYFSNNSETPIKGLHVGTPKLPAGISIQEFPEIESLAPGESTTTVMGINFCDSTQAANFQLCTQTRQFYVSIQPPVGELMAPVFMSENEFKKEQGKLTGMNEITEKLTLPDTCRSDHMVVQKVTATANLGRVPCGTSDEYRFAGRTLTSGSLVLLTLDARAAGAAQLTVNSEKMVIGTMLVKDVIQALTQ

Target ORF information:

RefSeq Version	XM_006540571.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 3, beta 2 subunit (Ap3b2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540571.4

ORF Insert Sequence:

ATGTCGGCCG CTCCGGCCTA CAGCGAAGAC AAGGGCGGCT CAGCCGGTCC CGGGGAGCCA 
GAATATGGCC ACGACCCGGC GAGCGGCGGT ATCTTCTCCT CGGACTACAA GCGGCATGAT 
GACCTGAAAG AGATGTTGGA CACCAACAAG GACTCCCTCA AGCTGGAGGC CATGAAGAGG 
ATTGTGGCAA TGATCGCCCG GGGAAAGAAT GCCTCGGACC TGTTCCCTGC AGTGGTGAAG 
AATGTGGCCT GTAAGAACAT AGAGGTGAAG AAGCTTGTCT ACGTGTATCT GGTCCGATAT 
GCCGAGGAGC AGCAAGACCT CGCTCTACTG TCCATCTCTA CTTTCCAACG TGGCCTAAAG 
GATCCCAACC AGCTGATCCG TGCCAGTGCG CTCCGGGTTC TCTCTAGCAT CCGTGTGCCC 
ATCATAGTGC CCATCATGAT GCTAGCCATC AAGGAAGCTG CCTCGGACAT GTCACCCTAC 
GTTCGGAAAA CAGCTGCTCA TGCCATCCCC AAACTCTACA GTTTGGACTC CGATCAGAAG 
GACCAGCTGA TAGAAGTCAT TGAGAAGCTT TTGGCAGATA AGACCACGCT GGTGGCAGGC 
AGTGTAGTGA TGGCCTTTGA AGAGGTATGT CCAGAACGTA TTGACCTGAT TCACAAAAAC 
TACCGGAAGC TCTGCAACCT GCTCATTGAT GTGGAGGAGT GGGGCCAGGT GGTGATTATC 
AGCATGCTCA CGCGTTATGC GCGAACGCAA TTCCTGAGCC CTACGCAGAA TGAGTCCTTG 
CTGGAGGAGA ATCCCGAGAA GGCCTTCTAT GGCTCCGAGG AGGACGAGGC CAAAGGCCCT 
GGATCAGAGG AGGCAGCCAC TGCGGCGCTG CCCGCTCGGA AGCCCTATGT AATGGACCCA 
GATCACCGGC TGCTGCTGCG GAACACCAAG CCCCTGCTGC AGAGCCGCAG CGCCGCGGTG 
GTCATGGCGG TGGCGCAGCT TTACTTCCAC CTAGCGCCTA AGGCAGAGGT GGGCGTCATC 
GCCAAGGCGC TGGTACGCCT GCTGCGCAGC CACAGTGAGG TGCAGTACGT AGTGCTCCAG 
AACGTGGCCA CCATGTCCAT CAAGCGCAGG GGTATGTTTG AACCCTACCT GAAGAGCTTC 
TACATTAGGT CCACGGATCC TACCCAGATC AAGATCCTGA AGCTGGAAGT GCTGACCAAC 
CTGGCCAATG AGACTAACAT CCCCACTGTT CTCCGGGAAT TCCAGACCTA CATTCGCAGC 
ATGGACAAAG ACTTTGTGGC AGCCACGATC CAGGCCATTG GACGATGTGC AACGAACATA 
GGCCGAGTCC GTGACACCTG TCTCAATGGC TTGGTGCAGC TTCTGTCCAA CCGTGACGAG 
CTTGTGGTTG CAGAGTCAGT GGTGGTCATT AAGAAGCTGC TGCAAATGCA GCCAGCGCAG 
CACGGTGAGA TCATCAAACA CTTGGCAAAG CTCACAGACA ACATCCAGGT GCCCATGGCA 
CGAGCCAGCA TCCTCTGGCT CATTGGAGAG TACTGTGAGC ATGTCCCCAA GATCGCACCT 
GATGTCCTGA GAAAAATGGC CAAGTCCTTC ACAGCAGAGG AGGACATCGT GAAGCTGCAG 
GTCATCAACC TGGCAGCCAA GCTGTACCTG ACTAACTCAA AGCAGACCAA GCTCTTGACC 
CAGTATGTGC TGAGTCTGGC CAAGTACGAC CAGAACTATG ATATCCGGGA TCGAGCCCGC 
TTCACCCGGC AGCTCATTGT TCCCTCTGAG CAGGGTGGTG CCCTCAGTCG CCATGCCAAG 
AAACTCTTCC TGGCCCCTAA GCCAGCCCCT ATCTTGGAGT CATCCTTCAA AGATCGTGAC 
CATTTCCAGC TGGGCTCACT GTCACACCTG CTCAATGCAA AGGCTACAGG CTACCAGGAG 
CTCCCGGACT GGCCAGAGGA AGCCCCAGAT CCATCTGTGC GCAATGTGGA GGAAGAAGAC 
CTCTCCCTTA TTGAGACCCA CGTGGGCCTG CTGGGCGAAT ACACTGAGGT ACCTGAATGG 
ACCAAATGTT CAAATCGGGA GAAGAGGAAG GAGAAGGAAA AGCCTTTCTA TTCTGACTCC 
GAGGGGGAGT CTGGCCCCAC TGAGTCTGCA GACAGCGAGC CTGAATCTGA GAGTGAGTCA 
GAGAGTAAGA GCAGCAGTGG GAGTGGCTCC GGGGAGTCTA GCAGTGAGTC GGACAATGAA 
GAAGAGGATG AGGAGAAAGG CGGAGGCAGC GAGAGTGAAC AGAGTGAGGA GGAAGATGAG 
AAGAAGAAGA AGACAAAGAA GAAGAAGGCA TCAGAGGGAC ACAGAGAAGG CTCATCATCA 
GAAGAGGGCA GTGACTCCAG CAGCAGCTCA GAGTCTGAGG TGACGTCAGA GTCGGAGGAG 
GAACAGGTGG AACCTGCCTC CTGGAGGAAG AAAACGCCTC CAGGCAGCAA GAGTGCCCCT 
GTAGCCAAGG AGATTTCCCT GCTGGACCTA GAGGATTTCA CCCCTCCTAG TGTCCAGCCT 
GTGTCTCCTC CCATGGTTGT GTCTACCAGT CTGGCTGCTG ACCTGGAAGG GCTGACACTC 
ACAGACTCCT CTCTGGTGCC TTCGCTGCTG AGTCCAGTGT CCAGCATTGG GAGGCAGGAG 
CTGCTGCACC GGGTAGCTGG AGAGGGGCTG TCTGTGGACT ATGCCTTCAG CCGCCAGCCT 
TTCTCTGGGG ATCCCCACAT GGTGTCTCTG CATATCTACT TCTCCAACAA CTCGGAGACT 
CCCATCAAGG GGCTGCATGT GGGCACTCCC AAGCTGCCTG CTGGCATCAG CATTCAAGAA 
TTCCCTGAAA TCGAGTCCCT GGCACCTGGA GAATCCACCA CTACTGTGAT GGGCATTAAC 
TTCTGCGACT CAACCCAGGC AGCCAACTTC CAGCTGTGCA CCCAAACCCG ACAGTTCTAT 
GTCTCCATTC AGCCACCTGT CGGGGAGCTG ATGGCCCCTG TATTTATGAG TGAGAATGAG 
TTCAAGAAGG AACAGGGAAA GCTGACAGGC ATGAATGAGA TCACAGAGAA GCTTACATTG 
CCAGACACCT GTCGAAGTGA TCACATGGTG GTGCAGAAAG TGACTGCCAC CGCCAACCTA 
GGTCGTGTCC CATGTGGGAC ATCTGATGAG TACAGGTTTG CAGGGAGGAC ATTAACTAGT 
GGGAGTCTGG TCCTGCTGAC TCTGGATGCC CGGGCAGCTG GAGCCGCCCA GCTGACCGTC 
AACAGTGAGA AGATGGTGAT TGGCACCATG CTGGTGAAGG ATGTGATTCA GGCTCTGACC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap3b2 ( NM_021492.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067467.2 Mus musculus adaptor-related protein complex 3, beta 2 subunit (Ap3b2), mRNA.

Price & Availability↑

CloneID	OMu12411
Clone ID Related Accession (Same CDS sequence)	NM_021492.3
Accession Version	NM_021492.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	AP-3 complex subunit beta-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ064603.1 and AY528675.1. On Aug 1, 2006 this sequence version replaced NM_021492.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY528675.1, SRR1660817.219919.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCGGCCG CTCCGGCCTA CAGCGAAGAC AAGGGCGGCT CAGCCGGTCC CGGGGAGCCA 
GAATATGGCC ACGACCCGGC GAGCGGCGGT ATCTTCTCCT CGGACTACAA GCGGCATGAT 
GACCTGAAAG AGATGTTGGA CACCAACAAG GACTCCCTCA AGCTGGAGGC CATGAAGAGG 
ATTGTGGCAA TGATCGCCCG GGGAAAGAAT GCCTCGGACC TGTTCCCTGC AGTGGTGAAG 
AATGTGGCCT GTAAGAACAT AGAGGTGAAG AAGCTTGTCT ACGTGTATCT GGTCCGATAT 
GCCGAGGAGC AGCAAGACCT CGCTCTACTG TCCATCTCTA CTTTCCAACG TGGCCTAAAG 
GATCCCAACC AGCTGATCCG TGCCAGTGCG CTCCGGGTTC TCTCTAGCAT CCGTGTGCCC 
ATCATAGTGC CCATCATGAT GCTAGCCATC AAGGAAGCTG CCTCGGACAT GTCACCCTAC 
GTTCGGAAAA CAGCTGCTCA TGCCATCCCC AAACTCTACA GTTTGGACTC CGATCAGAAG 
GACCAGCTGA TAGAAGTCAT TGAGAAGCTT TTGGCAGATA AGACCACGCT GGTGGCAGGC 
AGTGTAGTGA TGGCCTTTGA AGAGGTATGT CCAGAACGTA TTGACCTGAT TCACAAAAAC 
TACCGGAAGC TCTGCAACCT GCTCATTGAT GTGGAGGAGT GGGGCCAGGT GGTGATTATC 
AGCATGCTCA CGCGTTATGC GCGAACGCAA TTCCTGAGCC CTACGCAGAA TGAGTCCTTG 
CTGGAGGAGA ATCCCGAGAA GGCCTTCTAT GGCTCCGAGG AGGACGAGGC CAAAGGCCCT 
GGATCAGAGG AGGCAGCCAC TGCGGCGCTG CCCGCTCGGA AGCCCTATGT AATGGACCCA 
GATCACCGGC TGCTGCTGCG GAACACCAAG CCCCTGCTGC AGAGCCGCAG CGCCGCGGTG 
GTCATGGCGG TGGCGCAGCT TTACTTCCAC CTAGCGCCTA AGGCAGAGGT GGGCGTCATC 
GCCAAGGCGC TGGTACGCCT GCTGCGCAGC CACAGTGAGG TGCAGTACGT AGTGCTCCAG 
AACGTGGCCA CCATGTCCAT CAAGCGCAGG GGTATGTTTG AACCCTACCT GAAGAGCTTC 
TACATTAGGT CCACGGATCC TACCCAGATC AAGATCCTGA AGCTGGAAGT GCTGACCAAC 
CTGGCCAATG AGACTAACAT CCCCACTGTT CTCCGGGAAT TCCAGACCTA CATTCGCAGC 
ATGGACAAAG ACTTTGTGGC AGCCACGATC CAGGCCATTG GACGATGTGC AACGAACATA 
GGCCGAGTCC GTGACACCTG TCTCAATGGC TTGGTGCAGC TTCTGTCCAA CCGTGACGAG 
CTTGTGGTTG CAGAGTCAGT GGTGGTCATT AAGAAGCTGC TGCAAATGCA GCCAGCGCAG 
CACGGTGAGA TCATCAAACA CTTGGCAAAG CTCACAGACA ACATCCAGGT GCCCATGGCA 
CGAGCCAGCA TCCTCTGGCT CATTGGAGAG TACTGTGAGC ATGTCCCCAA GATCGCACCT 
GATGTCCTGA GAAAAATGGC CAAGTCCTTC ACAGCAGAGG AGGACATCGT GAAGCTGCAG 
GTCATCAACC TGGCAGCCAA GCTGTACCTG ACTAACTCAA AGCAGACCAA GCTCTTGACC 
CAGTATGTGC TGAGTCTGGC CAAGTACGAC CAGAACTATG ATATCCGGGA TCGAGCCCGC 
TTCACCCGGC AGCTCATTGT TCCCTCTGAG CAGGGTGGTG CCCTCAGTCG CCATGCCAAG 
AAACTCTTCC TGGCCCCTAA GCCAGCCCCT ATCTTGGAGT CATCCTTCAA AGATCGTGAC 
CATTTCCAGC TGGGCTCACT GTCACACCTG CTCAATGCAA AGGCTACAGG CTACCAGGAG 
CTCCCGGACT GGCCAGAGGA AGCCCCAGAT CCATCTGTGC GCAATGTGGA GGTACCTGAA 
TGGACCAAAT GTTCAAATCG GGAGAAGAGG AAGGAGAAGG AAAAGCCTTT CTATTCTGAC 
TCCGAGGGGG AGTCTGGCCC CACTGAGTCT GCAGACAGCG AGCCTGAATC TGAGAGTGAG 
TCAGAGAGTA AGAGCAGCAG TGGGAGTGGC TCCGGGGAGT CTAGCAGTGA GTCGGACAAT 
GAAGAAGAGG ATGAGGAGAA AGGCGGAGGC AGCGAGAGTG AACAGAGTGA GGAGGAAGAT 
GAGAAGAAGA AGAAGACAAA GAAGAAGAAG GCATCAGAGG GACACAGAGA AGGCTCATCA 
TCAGAAGAGG GCAGTGACTC CAGCAGCAGC TCAGAGTCTG AGGTGACGTC AGAGTCGGAG 
GAGGAACAGG TGGAACCTGC CTCCTGGAGG AAGAAAACGC CTCCAGGCAG CAAGAGTGCC 
CCTGTAGCCA AGGAGATTTC CCTGCTGGAC CTAGAGGATT TCACCCCTCC TAGTGTCCAG 
CCTGTGTCTC CTCCCATGGT TGTGTCTACC AGTCTGGCTG CTGACCTGGA AGGGCTGACA 
CTCACAGACT CCTCTCTGGT GCCTTCGCTG CTGAGTCCAG TGTCCAGCAT TGGGAGGCAG 
GAGCTGCTGC ACCGGGTAGC TGGAGAGGGG CTGTCTGTGG ACTATGCCTT CAGCCGCCAG 
CCTTTCTCTG GGGATCCCCA CATGGTGTCT CTGCATATCT ACTTCTCCAA CAACTCGGAG 
ACTCCCATCA AGGGGCTGCA TGTGGGCACT CCCAAGCTGC CTGCTGGCAT CAGCATTCAA 
GAATTCCCTG AAATCGAGTC CCTGGCACCT GGAGAATCCA CCACTACTGT GATGGGCATT 
AACTTCTGCG ACTCAACCCA GGCAGCCAAC TTCCAGCTGT GCACCCAAAC CCGACAGTTC 
TATGTCTCCA TTCAGCCACC TGTCGGGGAG CTGATGGCCC CTGTATTTAT GAGTGAGAAT 
GAGTTCAAGA AGGAACAGGG AAAGCTGACA GGCATGAATG AGATCACAGA GAAGCTTACA 
TTGCCAGACA CCTGTCGAAG TGATCACATG GTGGTGCAGA AAGTGACTGC CACCGCCAAC 
CTAGGTCGTG TCCCATGTGG GACATCTGAT GAGTACAGGT TTGCAGGGAG GACATTAACT 
AGTGGGAGTC TGGTCCTGCT GACTCTGGAT GCCCGGGCAG CTGGAGCCGC CCAGCTGACC 
GTCAACAGTG AGAAGATGGT GATTGGCACC ATGCTGGTGA AGGATGTGAT TCAGGCTCTG 
ACCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067467.2
CDS	306..3554
Translation	MSAAPAYSEDKGGSAGPGEPEYGHDPASGGIFSSDYKRHDDLKEMLDTNKDSLKLEAMKRIVAMIARGKNASDLFPAVVKNVACKNIEVKKLVYVYLVRYAEEQQDLALLSISTFQRGLKDPNQLIRASALRVLSSIRVPIIVPIMMLAIKEAASDMSPYVRKTAAHAIPKLYSLDSDQKDQLIEVIEKLLADKTTLVAGSVVMAFEEVCPERIDLIHKNYRKLCNLLIDVEEWGQVVIISMLTRYARTQFLSPTQNESLLEENPEKAFYGSEEDEAKGPGSEEAATAALPARKPYVMDPDHRLLLRNTKPLLQSRSAAVVMAVAQLYFHLAPKAEVGVIAKALVRLLRSHSEVQYVVLQNVATMSIKRRGMFEPYLKSFYIRSTDPTQIKILKLEVLTNLANETNIPTVLREFQTYIRSMDKDFVAATIQAIGRCATNIGRVRDTCLNGLVQLLSNRDELVVAESVVVIKKLLQMQPAQHGEIIKHLAKLTDNIQVPMARASILWLIGEYCEHVPKIAPDVLRKMAKSFTAEEDIVKLQVINLAAKLYLTNSKQTKLLTQYVLSLAKYDQNYDIRDRARFTRQLIVPSEQGGALSRHAKKLFLAPKPAPILESSFKDRDHFQLGSLSHLLNAKATGYQELPDWPEEAPDPSVRNVEVPEWTKCSNREKRKEKEKPFYSDSEGESGPTESADSEPESESESESKSSSGSGSGESSSESDNEEEDEEKGGGSESEQSEEEDEKKKKTKKKKASEGHREGSSSEEGSDSSSSSESEVTSESEEEQVEPASWRKKTPPGSKSAPVAKEISLLDLEDFTPPSVQPVSPPMVVSTSLAADLEGLTLTDSSLVPSLLSPVSSIGRQELLHRVAGEGLSVDYAFSRQPFSGDPHMVSLHIYFSNNSETPIKGLHVGTPKLPAGISIQEFPEIESLAPGESTTTVMGINFCDSTQAANFQLCTQTRQFYVSIQPPVGELMAPVFMSENEFKKEQGKLTGMNEITEKLTLPDTCRSDHMVVQKVTATANLGRVPCGTSDEYRFAGRTLTSGSLVLLTLDARAAGAAQLTVNSEKMVIGTMLVKDVIQALTQ

Target ORF information:

RefSeq Version	NM_021492.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 3, beta 2 subunit (Ap3b2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021492.3

ORF Insert Sequence:

ATGTCGGCCG CTCCGGCCTA CAGCGAAGAC AAGGGCGGCT CAGCCGGTCC CGGGGAGCCA 
GAATATGGCC ACGACCCGGC GAGCGGCGGT ATCTTCTCCT CGGACTACAA GCGGCATGAT 
GACCTGAAAG AGATGTTGGA CACCAACAAG GACTCCCTCA AGCTGGAGGC CATGAAGAGG 
ATTGTGGCAA TGATCGCCCG GGGAAAGAAT GCCTCGGACC TGTTCCCTGC AGTGGTGAAG 
AATGTGGCCT GTAAGAACAT AGAGGTGAAG AAGCTTGTCT ACGTGTATCT GGTCCGATAT 
GCCGAGGAGC AGCAAGACCT CGCTCTACTG TCCATCTCTA CTTTCCAACG TGGCCTAAAG 
GATCCCAACC AGCTGATCCG TGCCAGTGCG CTCCGGGTTC TCTCTAGCAT CCGTGTGCCC 
ATCATAGTGC CCATCATGAT GCTAGCCATC AAGGAAGCTG CCTCGGACAT GTCACCCTAC 
GTTCGGAAAA CAGCTGCTCA TGCCATCCCC AAACTCTACA GTTTGGACTC CGATCAGAAG 
GACCAGCTGA TAGAAGTCAT TGAGAAGCTT TTGGCAGATA AGACCACGCT GGTGGCAGGC 
AGTGTAGTGA TGGCCTTTGA AGAGGTATGT CCAGAACGTA TTGACCTGAT TCACAAAAAC 
TACCGGAAGC TCTGCAACCT GCTCATTGAT GTGGAGGAGT GGGGCCAGGT GGTGATTATC 
AGCATGCTCA CGCGTTATGC GCGAACGCAA TTCCTGAGCC CTACGCAGAA TGAGTCCTTG 
CTGGAGGAGA ATCCCGAGAA GGCCTTCTAT GGCTCCGAGG AGGACGAGGC CAAAGGCCCT 
GGATCAGAGG AGGCAGCCAC TGCGGCGCTG CCCGCTCGGA AGCCCTATGT AATGGACCCA 
GATCACCGGC TGCTGCTGCG GAACACCAAG CCCCTGCTGC AGAGCCGCAG CGCCGCGGTG 
GTCATGGCGG TGGCGCAGCT TTACTTCCAC CTAGCGCCTA AGGCAGAGGT GGGCGTCATC 
GCCAAGGCGC TGGTACGCCT GCTGCGCAGC CACAGTGAGG TGCAGTACGT AGTGCTCCAG 
AACGTGGCCA CCATGTCCAT CAAGCGCAGG GGTATGTTTG AACCCTACCT GAAGAGCTTC 
TACATTAGGT CCACGGATCC TACCCAGATC AAGATCCTGA AGCTGGAAGT GCTGACCAAC 
CTGGCCAATG AGACTAACAT CCCCACTGTT CTCCGGGAAT TCCAGACCTA CATTCGCAGC 
ATGGACAAAG ACTTTGTGGC AGCCACGATC CAGGCCATTG GACGATGTGC AACGAACATA 
GGCCGAGTCC GTGACACCTG TCTCAATGGC TTGGTGCAGC TTCTGTCCAA CCGTGACGAG 
CTTGTGGTTG CAGAGTCAGT GGTGGTCATT AAGAAGCTGC TGCAAATGCA GCCAGCGCAG 
CACGGTGAGA TCATCAAACA CTTGGCAAAG CTCACAGACA ACATCCAGGT GCCCATGGCA 
CGAGCCAGCA TCCTCTGGCT CATTGGAGAG TACTGTGAGC ATGTCCCCAA GATCGCACCT 
GATGTCCTGA GAAAAATGGC CAAGTCCTTC ACAGCAGAGG AGGACATCGT GAAGCTGCAG 
GTCATCAACC TGGCAGCCAA GCTGTACCTG ACTAACTCAA AGCAGACCAA GCTCTTGACC 
CAGTATGTGC TGAGTCTGGC CAAGTACGAC CAGAACTATG ATATCCGGGA TCGAGCCCGC 
TTCACCCGGC AGCTCATTGT TCCCTCTGAG CAGGGTGGTG CCCTCAGTCG CCATGCCAAG 
AAACTCTTCC TGGCCCCTAA GCCAGCCCCT ATCTTGGAGT CATCCTTCAA AGATCGTGAC 
CATTTCCAGC TGGGCTCACT GTCACACCTG CTCAATGCAA AGGCTACAGG CTACCAGGAG 
CTCCCGGACT GGCCAGAGGA AGCCCCAGAT CCATCTGTGC GCAATGTGGA GGTACCTGAA 
TGGACCAAAT GTTCAAATCG GGAGAAGAGG AAGGAGAAGG AAAAGCCTTT CTATTCTGAC 
TCCGAGGGGG AGTCTGGCCC CACTGAGTCT GCAGACAGCG AGCCTGAATC TGAGAGTGAG 
TCAGAGAGTA AGAGCAGCAG TGGGAGTGGC TCCGGGGAGT CTAGCAGTGA GTCGGACAAT 
GAAGAAGAGG ATGAGGAGAA AGGCGGAGGC AGCGAGAGTG AACAGAGTGA GGAGGAAGAT 
GAGAAGAAGA AGAAGACAAA GAAGAAGAAG GCATCAGAGG GACACAGAGA AGGCTCATCA 
TCAGAAGAGG GCAGTGACTC CAGCAGCAGC TCAGAGTCTG AGGTGACGTC AGAGTCGGAG 
GAGGAACAGG TGGAACCTGC CTCCTGGAGG AAGAAAACGC CTCCAGGCAG CAAGAGTGCC 
CCTGTAGCCA AGGAGATTTC CCTGCTGGAC CTAGAGGATT TCACCCCTCC TAGTGTCCAG 
CCTGTGTCTC CTCCCATGGT TGTGTCTACC AGTCTGGCTG CTGACCTGGA AGGGCTGACA 
CTCACAGACT CCTCTCTGGT GCCTTCGCTG CTGAGTCCAG TGTCCAGCAT TGGGAGGCAG 
GAGCTGCTGC ACCGGGTAGC TGGAGAGGGG CTGTCTGTGG ACTATGCCTT CAGCCGCCAG 
CCTTTCTCTG GGGATCCCCA CATGGTGTCT CTGCATATCT ACTTCTCCAA CAACTCGGAG 
ACTCCCATCA AGGGGCTGCA TGTGGGCACT CCCAAGCTGC CTGCTGGCAT CAGCATTCAA 
GAATTCCCTG AAATCGAGTC CCTGGCACCT GGAGAATCCA CCACTACTGT GATGGGCATT 
AACTTCTGCG ACTCAACCCA GGCAGCCAAC TTCCAGCTGT GCACCCAAAC CCGACAGTTC 
TATGTCTCCA TTCAGCCACC TGTCGGGGAG CTGATGGCCC CTGTATTTAT GAGTGAGAAT 
GAGTTCAAGA AGGAACAGGG AAAGCTGACA GGCATGAATG AGATCACAGA GAAGCTTACA 
TTGCCAGACA CCTGTCGAAG TGATCACATG GTGGTGCAGA AAGTGACTGC CACCGCCAAC 
CTAGGTCGTG TCCCATGTGG GACATCTGAT GAGTACAGGT TTGCAGGGAG GACATTAACT 
AGTGGGAGTC TGGTCCTGCT GACTCTGGAT GCCCGGGCAG CTGGAGCCGC CCAGCTGACC 
GTCAACAGTG AGAAGATGGT GATTGGCACC ATGCTGGTGA AGGATGTGAT TCAGGCTCTG 
ACCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap3b2 ( XM_006540571.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540634.1 Mus musculus adaptor-related protein complex 3, beta 2 subunit (Ap3b2), transcript variant X1, mRNA.

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CloneID	OMu38642
Clone ID Related Accession (Same CDS sequence)	XM_006540571.3 , XM_006540571.4
Accession Version	XM_006540571.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	AP-3 complex subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540571.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCG CTCCGGCCTA CAGCGAAGAC AAGGGCGGCT CAGCCGGTCC CGGGGAGCCA 
GAATATGGCC ACGACCCGGC GAGCGGCGGT ATCTTCTCCT CGGACTACAA GCGGCATGAT 
GACCTGAAAG AGATGTTGGA CACCAACAAG GACTCCCTCA AGCTGGAGGC CATGAAGAGG 
ATTGTGGCAA TGATCGCCCG GGGAAAGAAT GCCTCGGACC TGTTCCCTGC AGTGGTGAAG 
AATGTGGCCT GTAAGAACAT AGAGGTGAAG AAGCTTGTCT ACGTGTATCT GGTCCGATAT 
GCCGAGGAGC AGCAAGACCT CGCTCTACTG TCCATCTCTA CTTTCCAACG TGGCCTAAAG 
GATCCCAACC AGCTGATCCG TGCCAGTGCG CTCCGGGTTC TCTCTAGCAT CCGTGTGCCC 
ATCATAGTGC CCATCATGAT GCTAGCCATC AAGGAAGCTG CCTCGGACAT GTCACCCTAC 
GTTCGGAAAA CAGCTGCTCA TGCCATCCCC AAACTCTACA GTTTGGACTC CGATCAGAAG 
GACCAGCTGA TAGAAGTCAT TGAGAAGCTT TTGGCAGATA AGACCACGCT GGTGGCAGGC 
AGTGTAGTGA TGGCCTTTGA AGAGGTATGT CCAGAACGTA TTGACCTGAT TCACAAAAAC 
TACCGGAAGC TCTGCAACCT GCTCATTGAT GTGGAGGAGT GGGGCCAGGT GGTGATTATC 
AGCATGCTCA CGCGTTATGC GCGAACGCAA TTCCTGAGCC CTACGCAGAA TGAGTCCTTG 
CTGGAGGAGA ATCCCGAGAA GGCCTTCTAT GGCTCCGAGG AGGACGAGGC CAAAGGCCCT 
GGATCAGAGG AGGCAGCCAC TGCGGCGCTG CCCGCTCGGA AGCCCTATGT AATGGACCCA 
GATCACCGGC TGCTGCTGCG GAACACCAAG CCCCTGCTGC AGAGCCGCAG CGCCGCGGTG 
GTCATGGCGG TGGCGCAGCT TTACTTCCAC CTAGCGCCTA AGGCAGAGGT GGGCGTCATC 
GCCAAGGCGC TGGTACGCCT GCTGCGCAGC CACAGTGAGG TGCAGTACGT AGTGCTCCAG 
AACGTGGCCA CCATGTCCAT CAAGCGCAGG GGTATGTTTG AACCCTACCT GAAGAGCTTC 
TACATTAGGT CCACGGATCC TACCCAGATC AAGATCCTGA AGCTGGAAGT GCTGACCAAC 
CTGGCCAATG AGACTAACAT CCCCACTGTT CTCCGGGAAT TCCAGACCTA CATTCGCAGC 
ATGGACAAAG ACTTTGTGGC AGCCACGATC CAGGCCATTG GACGATGTGC AACGAACATA 
GGCCGAGTCC GTGACACCTG TCTCAATGGC TTGGTGCAGC TTCTGTCCAA CCGTGACGAG 
CTTGTGGTTG CAGAGTCAGT GGTGGTCATT AAGAAGCTGC TGCAAATGCA GCCAGCGCAG 
CACGGTGAGA TCATCAAACA CTTGGCAAAG CTCACAGACA ACATCCAGGT GCCCATGGCA 
CGAGCCAGCA TCCTCTGGCT CATTGGAGAG TACTGTGAGC ATGTCCCCAA GATCGCACCT 
GATGTCCTGA GAAAAATGGC CAAGTCCTTC ACAGCAGAGG AGGACATCGT GAAGCTGCAG 
GTCATCAACC TGGCAGCCAA GCTGTACCTG ACTAACTCAA AGCAGACCAA GCTCTTGACC 
CAGTATGTGC TGAGTCTGGC CAAGTACGAC CAGAACTATG ATATCCGGGA TCGAGCCCGC 
TTCACCCGGC AGCTCATTGT TCCCTCTGAG CAGGGTGGTG CCCTCAGTCG CCATGCCAAG 
AAACTCTTCC TGGCCCCTAA GCCAGCCCCT ATCTTGGAGT CATCCTTCAA AGATCGTGAC 
CATTTCCAGC TGGGCTCACT GTCACACCTG CTCAATGCAA AGGCTACAGG CTACCAGGAG 
CTCCCGGACT GGCCAGAGGA AGCCCCAGAT CCATCTGTGC GCAATGTGGA GGAAGAAGAC 
CTCTCCCTTA TTGAGACCCA CGTGGGCCTG CTGGGCGAAT ACACTGAGGT ACCTGAATGG 
ACCAAATGTT CAAATCGGGA GAAGAGGAAG GAGAAGGAAA AGCCTTTCTA TTCTGACTCC 
GAGGGGGAGT CTGGCCCCAC TGAGTCTGCA GACAGCGAGC CTGAATCTGA GAGTGAGTCA 
GAGAGTAAGA GCAGCAGTGG GAGTGGCTCC GGGGAGTCTA GCAGTGAGTC GGACAATGAA 
GAAGAGGATG AGGAGAAAGG CGGAGGCAGC GAGAGTGAAC AGAGTGAGGA GGAAGATGAG 
AAGAAGAAGA AGACAAAGAA GAAGAAGGCA TCAGAGGGAC ACAGAGAAGG CTCATCATCA 
GAAGAGGGCA GTGACTCCAG CAGCAGCTCA GAGTCTGAGG TGACGTCAGA GTCGGAGGAG 
GAACAGGTGG AACCTGCCTC CTGGAGGAAG AAAACGCCTC CAGGCAGCAA GAGTGCCCCT 
GTAGCCAAGG AGATTTCCCT GCTGGACCTA GAGGATTTCA CCCCTCCTAG TGTCCAGCCT 
GTGTCTCCTC CCATGGTTGT GTCTACCAGT CTGGCTGCTG ACCTGGAAGG GCTGACACTC 
ACAGACTCCT CTCTGGTGCC TTCGCTGCTG AGTCCAGTGT CCAGCATTGG GAGGCAGGAG 
CTGCTGCACC GGGTAGCTGG AGAGGGGCTG TCTGTGGACT ATGCCTTCAG CCGCCAGCCT 
TTCTCTGGGG ATCCCCACAT GGTGTCTCTG CATATCTACT TCTCCAACAA CTCGGAGACT 
CCCATCAAGG GGCTGCATGT GGGCACTCCC AAGCTGCCTG CTGGCATCAG CATTCAAGAA 
TTCCCTGAAA TCGAGTCCCT GGCACCTGGA GAATCCACCA CTACTGTGAT GGGCATTAAC 
TTCTGCGACT CAACCCAGGC AGCCAACTTC CAGCTGTGCA CCCAAACCCG ACAGTTCTAT 
GTCTCCATTC AGCCACCTGT CGGGGAGCTG ATGGCCCCTG TATTTATGAG TGAGAATGAG 
TTCAAGAAGG AACAGGGAAA GCTGACAGGC ATGAATGAGA TCACAGAGAA GCTTACATTG 
CCAGACACCT GTCGAAGTGA TCACATGGTG GTGCAGAAAG TGACTGCCAC CGCCAACCTA 
GGTCGTGTCC CATGTGGGAC ATCTGATGAG TACAGGTTTG CAGGGAGGAC ATTAACTAGT 
GGGAGTCTGG TCCTGCTGAC TCTGGATGCC CGGGCAGCTG GAGCCGCCCA GCTGACCGTC 
AACAGTGAGA AGATGGTGAT TGGCACCATG CTGGTGAAGG ATGTGATTCA GGCTCTGACC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540634.1
CDS	418..3723
Translation	MSAAPAYSEDKGGSAGPGEPEYGHDPASGGIFSSDYKRHDDLKEMLDTNKDSLKLEAMKRIVAMIARGKNASDLFPAVVKNVACKNIEVKKLVYVYLVRYAEEQQDLALLSISTFQRGLKDPNQLIRASALRVLSSIRVPIIVPIMMLAIKEAASDMSPYVRKTAAHAIPKLYSLDSDQKDQLIEVIEKLLADKTTLVAGSVVMAFEEVCPERIDLIHKNYRKLCNLLIDVEEWGQVVIISMLTRYARTQFLSPTQNESLLEENPEKAFYGSEEDEAKGPGSEEAATAALPARKPYVMDPDHRLLLRNTKPLLQSRSAAVVMAVAQLYFHLAPKAEVGVIAKALVRLLRSHSEVQYVVLQNVATMSIKRRGMFEPYLKSFYIRSTDPTQIKILKLEVLTNLANETNIPTVLREFQTYIRSMDKDFVAATIQAIGRCATNIGRVRDTCLNGLVQLLSNRDELVVAESVVVIKKLLQMQPAQHGEIIKHLAKLTDNIQVPMARASILWLIGEYCEHVPKIAPDVLRKMAKSFTAEEDIVKLQVINLAAKLYLTNSKQTKLLTQYVLSLAKYDQNYDIRDRARFTRQLIVPSEQGGALSRHAKKLFLAPKPAPILESSFKDRDHFQLGSLSHLLNAKATGYQELPDWPEEAPDPSVRNVEEEDLSLIETHVGLLGEYTEVPEWTKCSNREKRKEKEKPFYSDSEGESGPTESADSEPESESESESKSSSGSGSGESSSESDNEEEDEEKGGGSESEQSEEEDEKKKKTKKKKASEGHREGSSSEEGSDSSSSSESEVTSESEEEQVEPASWRKKTPPGSKSAPVAKEISLLDLEDFTPPSVQPVSPPMVVSTSLAADLEGLTLTDSSLVPSLLSPVSSIGRQELLHRVAGEGLSVDYAFSRQPFSGDPHMVSLHIYFSNNSETPIKGLHVGTPKLPAGISIQEFPEIESLAPGESTTTVMGINFCDSTQAANFQLCTQTRQFYVSIQPPVGELMAPVFMSENEFKKEQGKLTGMNEITEKLTLPDTCRSDHMVVQKVTATANLGRVPCGTSDEYRFAGRTLTSGSLVLLTLDARAAGAAQLTVNSEKMVIGTMLVKDVIQALTQ

Target ORF information:

RefSeq Version	XM_006540571.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 3, beta 2 subunit (Ap3b2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540571.3

ORF Insert Sequence:

ATGTCGGCCG CTCCGGCCTA CAGCGAAGAC AAGGGCGGCT CAGCCGGTCC CGGGGAGCCA 
GAATATGGCC ACGACCCGGC GAGCGGCGGT ATCTTCTCCT CGGACTACAA GCGGCATGAT 
GACCTGAAAG AGATGTTGGA CACCAACAAG GACTCCCTCA AGCTGGAGGC CATGAAGAGG 
ATTGTGGCAA TGATCGCCCG GGGAAAGAAT GCCTCGGACC TGTTCCCTGC AGTGGTGAAG 
AATGTGGCCT GTAAGAACAT AGAGGTGAAG AAGCTTGTCT ACGTGTATCT GGTCCGATAT 
GCCGAGGAGC AGCAAGACCT CGCTCTACTG TCCATCTCTA CTTTCCAACG TGGCCTAAAG 
GATCCCAACC AGCTGATCCG TGCCAGTGCG CTCCGGGTTC TCTCTAGCAT CCGTGTGCCC 
ATCATAGTGC CCATCATGAT GCTAGCCATC AAGGAAGCTG CCTCGGACAT GTCACCCTAC 
GTTCGGAAAA CAGCTGCTCA TGCCATCCCC AAACTCTACA GTTTGGACTC CGATCAGAAG 
GACCAGCTGA TAGAAGTCAT TGAGAAGCTT TTGGCAGATA AGACCACGCT GGTGGCAGGC 
AGTGTAGTGA TGGCCTTTGA AGAGGTATGT CCAGAACGTA TTGACCTGAT TCACAAAAAC 
TACCGGAAGC TCTGCAACCT GCTCATTGAT GTGGAGGAGT GGGGCCAGGT GGTGATTATC 
AGCATGCTCA CGCGTTATGC GCGAACGCAA TTCCTGAGCC CTACGCAGAA TGAGTCCTTG 
CTGGAGGAGA ATCCCGAGAA GGCCTTCTAT GGCTCCGAGG AGGACGAGGC CAAAGGCCCT 
GGATCAGAGG AGGCAGCCAC TGCGGCGCTG CCCGCTCGGA AGCCCTATGT AATGGACCCA 
GATCACCGGC TGCTGCTGCG GAACACCAAG CCCCTGCTGC AGAGCCGCAG CGCCGCGGTG 
GTCATGGCGG TGGCGCAGCT TTACTTCCAC CTAGCGCCTA AGGCAGAGGT GGGCGTCATC 
GCCAAGGCGC TGGTACGCCT GCTGCGCAGC CACAGTGAGG TGCAGTACGT AGTGCTCCAG 
AACGTGGCCA CCATGTCCAT CAAGCGCAGG GGTATGTTTG AACCCTACCT GAAGAGCTTC 
TACATTAGGT CCACGGATCC TACCCAGATC AAGATCCTGA AGCTGGAAGT GCTGACCAAC 
CTGGCCAATG AGACTAACAT CCCCACTGTT CTCCGGGAAT TCCAGACCTA CATTCGCAGC 
ATGGACAAAG ACTTTGTGGC AGCCACGATC CAGGCCATTG GACGATGTGC AACGAACATA 
GGCCGAGTCC GTGACACCTG TCTCAATGGC TTGGTGCAGC TTCTGTCCAA CCGTGACGAG 
CTTGTGGTTG CAGAGTCAGT GGTGGTCATT AAGAAGCTGC TGCAAATGCA GCCAGCGCAG 
CACGGTGAGA TCATCAAACA CTTGGCAAAG CTCACAGACA ACATCCAGGT GCCCATGGCA 
CGAGCCAGCA TCCTCTGGCT CATTGGAGAG TACTGTGAGC ATGTCCCCAA GATCGCACCT 
GATGTCCTGA GAAAAATGGC CAAGTCCTTC ACAGCAGAGG AGGACATCGT GAAGCTGCAG 
GTCATCAACC TGGCAGCCAA GCTGTACCTG ACTAACTCAA AGCAGACCAA GCTCTTGACC 
CAGTATGTGC TGAGTCTGGC CAAGTACGAC CAGAACTATG ATATCCGGGA TCGAGCCCGC 
TTCACCCGGC AGCTCATTGT TCCCTCTGAG CAGGGTGGTG CCCTCAGTCG CCATGCCAAG 
AAACTCTTCC TGGCCCCTAA GCCAGCCCCT ATCTTGGAGT CATCCTTCAA AGATCGTGAC 
CATTTCCAGC TGGGCTCACT GTCACACCTG CTCAATGCAA AGGCTACAGG CTACCAGGAG 
CTCCCGGACT GGCCAGAGGA AGCCCCAGAT CCATCTGTGC GCAATGTGGA GGAAGAAGAC 
CTCTCCCTTA TTGAGACCCA CGTGGGCCTG CTGGGCGAAT ACACTGAGGT ACCTGAATGG 
ACCAAATGTT CAAATCGGGA GAAGAGGAAG GAGAAGGAAA AGCCTTTCTA TTCTGACTCC 
GAGGGGGAGT CTGGCCCCAC TGAGTCTGCA GACAGCGAGC CTGAATCTGA GAGTGAGTCA 
GAGAGTAAGA GCAGCAGTGG GAGTGGCTCC GGGGAGTCTA GCAGTGAGTC GGACAATGAA 
GAAGAGGATG AGGAGAAAGG CGGAGGCAGC GAGAGTGAAC AGAGTGAGGA GGAAGATGAG 
AAGAAGAAGA AGACAAAGAA GAAGAAGGCA TCAGAGGGAC ACAGAGAAGG CTCATCATCA 
GAAGAGGGCA GTGACTCCAG CAGCAGCTCA GAGTCTGAGG TGACGTCAGA GTCGGAGGAG 
GAACAGGTGG AACCTGCCTC CTGGAGGAAG AAAACGCCTC CAGGCAGCAA GAGTGCCCCT 
GTAGCCAAGG AGATTTCCCT GCTGGACCTA GAGGATTTCA CCCCTCCTAG TGTCCAGCCT 
GTGTCTCCTC CCATGGTTGT GTCTACCAGT CTGGCTGCTG ACCTGGAAGG GCTGACACTC 
ACAGACTCCT CTCTGGTGCC TTCGCTGCTG AGTCCAGTGT CCAGCATTGG GAGGCAGGAG 
CTGCTGCACC GGGTAGCTGG AGAGGGGCTG TCTGTGGACT ATGCCTTCAG CCGCCAGCCT 
TTCTCTGGGG ATCCCCACAT GGTGTCTCTG CATATCTACT TCTCCAACAA CTCGGAGACT 
CCCATCAAGG GGCTGCATGT GGGCACTCCC AAGCTGCCTG CTGGCATCAG CATTCAAGAA 
TTCCCTGAAA TCGAGTCCCT GGCACCTGGA GAATCCACCA CTACTGTGAT GGGCATTAAC 
TTCTGCGACT CAACCCAGGC AGCCAACTTC CAGCTGTGCA CCCAAACCCG ACAGTTCTAT 
GTCTCCATTC AGCCACCTGT CGGGGAGCTG ATGGCCCCTG TATTTATGAG TGAGAATGAG 
TTCAAGAAGG AACAGGGAAA GCTGACAGGC ATGAATGAGA TCACAGAGAA GCTTACATTG 
CCAGACACCT GTCGAAGTGA TCACATGGTG GTGCAGAAAG TGACTGCCAC CGCCAACCTA 
GGTCGTGTCC CATGTGGGAC ATCTGATGAG TACAGGTTTG CAGGGAGGAC ATTAACTAGT 
GGGAGTCTGG TCCTGCTGAC TCTGGATGCC CGGGCAGCTG GAGCCGCCCA GCTGACCGTC 
AACAGTGAGA AGATGGTGAT TGGCACCATG CTGGTGAAGG ATGTGATTCA GGCTCTGACC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sporadic Hirschsprung Disease: Mutational Spectrum and Novel Candidate Genes Revealed by Next-generation Sequencing.
Scientific reports7(1)14796(2017 Nov)
Zhang Z,Li Q,Diao M,Liu N,Cheng W,Xiao P,Zou J,Su L,Yu K,Wu J,Li L,Jiang Q

Exome sequencing reveals pathogenic mutations in 91 strains of mice with Mendelian disorders.
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