gRNAs
The following Slc19a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc19a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu09571 | |
| Clone ID Related Accession (Same CDS sequence) | NM_054087.3 | |
| Accession Version | NM_054087.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-24 | |
| Organism | Mus musculus(house mouse) | |
| Product | thiamine transporter 1 isoform a | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK044825.1 and BC034659.1. On Feb 14, 2013 this sequence version replaced NM_054087.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK044825.1, SRR1660815.270789.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGACGTGC CAGCCAGGGT GTCCAGACGA GCGGCGGCGG CGGCGGCCAG GATGCTTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_473428.1 |
| CDS | 208..1704 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_054087.3 |
1 | ATGGACGTGC CAGCCAGGGT GTCCAGACGA GCGGCGGCGG CGGCGGCCAG GATGCTTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu17115 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001276455.1 | |
| Accession Version | NM_001276455.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-23 | |
| Organism | Mus musculus(house mouse) | |
| Product | thiamine transporter 1 isoform b | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK044825.1, AC105161.10 and BC034659.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (b) is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF418986.1, AF326916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGACGTGC CAGCCAGGGT GTCCAGACGA GCGGCGGCGG CGGCGGCCAG GATGCTTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001263384.1 |
| CDS | 208..1590 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001276455.1 |
1 | ATGGACGTGC CAGCCAGGGT GTCCAGACGA GCGGCGGCGG CGGCGGCCAG GATGCTTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Relative contribution of THTR-1 and THTR-2 in thiamin uptake by pancreatic acinar cells: studies utilizing Slc19a2 and Slc19a3 knockout mouse models. |
Inner hair cells are not required for survival of spiral ganglion neurons in the adult cochlea. |
Impaired intestinal vitamin B1 (thiamin) uptake in thiamin transporter-2-deficient mice. |
Pancreatic beta cells and islets take up thiamin by a regulated carrier-mediated process: studies using mice and human pancreatic preparations. |
Deletion of SLC19A2, the high affinity thiamine transporter, causes selective inner hair cell loss and an auditory neuropathy phenotype. |
