Alox12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alox12
Entrez Gene ID	11684
Full Name	arachidonate 12-lipoxygenase
Synonyms	9930022G08Rik,Alox12p,P-12LO
General protein information	Preferred Names arachidonate 12-lipoxygenase Names arachidonate 12-lipoxygenase, 12S-type 12S-LOX 12S-lipoxygenase lipoxin synthase 12-LO platelet-type lipoxygenase 12
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B3\|11 42.99 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001331118.1	NP_001318047.1	arachidonate 12-lipoxygenase, 12S-type isoform 2
NM_007440.5	NP_031466.2	arachidonate 12-lipoxygenase, 12S-type isoform 1
XM_011248678.1	XP_011246980.1	arachidonate 12-lipoxygenase, 12S-type isoform X1

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_08499	XP_003716006.1
	ALOX12	XP_001104192.1
Mus musculus (house mouse)	Alox12	NP_031466.2
Danio rerio (zebrafish)	alox12	NP_955912.1
Pan troglodytes (chimpanzee)	ALOX12	XP_511984.3
Canis lupus familiaris (dog)	ALOX12	XP_536613.2
Rattus norvegicus (Norway rat)	Alox12	NP_001099268.1
Homo sapiens (human)	ALOX12	NP_000688.2
Bos taurus (cattle)	ALOX12	NP_001179265.1

Gene Ontology
Bibliography

Related articles in PubMed

An ALOX12-12-HETE-GPR31 signaling axis is a key mediator of hepatic ischemia-reperfusion injury.
Zhang XJ, Cheng X, Yan ZZ, Fang J, Wang X, Wang W, Liu ZY, Shen LJ, Zhang P, Wang PX, Liao R, Ji YX, Wang JY, Tian S, Zhu XY, Zhang Y, Tian RF, Wang L, Ma XL, Huang Z, She ZG, Li H
Nature medicine24(1)73-83(2018 Jan)

Networks of enzymatically oxidized membrane lipids support calcium-dependent coagulation factor binding to maintain hemostasis.
Lauder SN, Allen-Redpath K, Slatter DA, Aldrovandi M, O'Connor A, Farewell D, Percy CL, Molhoek JE, Rannikko S, Tyrrell VJ, Ferla S, Milne GL, Poole AW, Thomas CP, Obaji S, Taylor PR, Jones SA, de Groot PG, Urbanus RT, H?rkk? S, Uderhardt S, Ackermann J, Vince Jenkins P, Brancale A, Kr?nke G, Collins PW, O'Donnell VB
Science signaling10(507)(2017 Nov)

12(S)-HETrE, a 12-Lipoxygenase Oxylipin of Dihomo-?-Linolenic Acid, Inhibits Thrombosis via G?s Signaling in Platelets.
Yeung J, Tourdot BE, Adili R, Green AR, Freedman CJ, Fernandez-Perez P, Yu J, Holman TR, Holinstat M
Arteriosclerosis, thrombosis, and vascular biology36(10)2068-77(2016 Oct)

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
Wu Y, Wei FY, Kawarada L, Suzuki T, Araki K, Komohara Y, Fujimura A, Kaitsuka T, Takeya M, Oike Y, Suzuki T, Tomizawa K
PLoS genetics12(9)e1006355(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

12(S)-HETrE, a 12-lipoxygenase oxylipin of dihomo-gamma-linolenic acid, inhibits thrombosis via Galphas signaling in platelets.
Title: 12(S)-HETrE, a 12-Lipoxygenase Oxylipin of Dihomo-?-Linolenic Acid, Inhibits Thrombosis via G?s Signaling in Platelets.

Data show that the coordinated action of phospholipase A2 IIA (sPLA2-IIA) and 12-lipoxygenase (12-LO) promotes inflammatory arthritis.
Title: Platelet microparticles are internalized in neutrophils via the concerted activity of 12-lipoxygenase and secreted phospholipase A2-IIA.

the amount of LPL expressed in muscle and heart governed both the binding of chylomicron particles and the assimilation of chylomicron lipids in the tissue.
Title: A lipidomic screen of hyperglycemia-treated HRECs links 12/15-Lipoxygenase to microvascular dysfunction during diabetic retinopathy via NADPH oxidase.

IGF-1 reduces lipid oxidation and foam cell formation via downregulation of 12/15-LOX to prevent atherosclerosis.
Title: Insulin-like growth factor I reduces lipid oxidation and foam cell formation via downregulation of 12/15-lipoxygenase.

Data indicate that 12/15-LO knockout (KO) mice were protected from high fat (HF) diet with high dietary omega6/omega3 ratio (11ratio1, HFH)-induced fatty liver.
Title: Reduced dietary omega-6 to omega-3 fatty acid ratio and 12/15-lipoxygenase deficiency are protective against chronic high fat diet-induced steatohepatitis.

The following Alox12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alox12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00181	NM_007440.5 Latest version!	Mus musculus arachidonate 12-lipoxygenase (Alox12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$391.30 $559.00
OMu67093	NM_001331118.1 Latest version!	Mus musculus arachidonate 12-lipoxygenase (Alox12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu67093	XM_011248678.1 Latest version!	Mus musculus arachidonate 12-lipoxygenase (Alox12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alox12 ( NM_007440.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031466.2 Mus musculus arachidonate 12-lipoxygenase (Alox12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00181
Clone ID Related Accession (Same CDS sequence)	NM_007440.5
Accession Version	NM_007440.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	arachidonate 12-lipoxygenase, 12S-type isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL669869.10 and AK087724.1. On Sep 8, 2016 this sequence version replaced NM_007440.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK087724.1, AK036898.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCGCT ACCGCGTCCG TGTGGTCACT GGGGCCTGGC TCTTCTCCGG ATCCCTCAAC 
CTAGTGCGTT TGTGGCTGGT TGGGGAGCAC CGGGAGGCGA AACTGGAGCT GCAACTGCGG 
CCAGCGCGGG GCAAGGAGGA GGAGTTTGAC TTCGACGTTC CCGAGGACTT GGGGCCACTG 
CAGTTTGTGA AACTGCACAA ACAGCACACA GTTGTGGATG ACGCCTGGTT CTGCAACCTC 
ATCACAGTTC AGGGGCCGGG GACAAGTGCA GAGGCCGTGT TTCCCTGCTA CCGCTGGGTG 
CAGGGAGAGG GAATCCTGAG CCTCCCGGAG GGGACAGCCC GCCTGGCAGG AGACAATGCC 
TTAGATGTCT TCCAGAAGTA TCGAGAAAAG GAACTGAAGG AAAGACAACA GACCTACTGC 
TGGGCCACCT GGAAAGAAGG CTTACCTCAG ACAATAGCAG CGGACTGTAA GGATGACCTT 
CCTCCAAATA TGAGATTCCA TGAGGAGAAG AGACTGGACT TTGAATGGAC GTTGAAGGCG 
GGGGTTCTGG AAATGGGCCT CAAACGTGTT TATACCCTCC TGAGAAGCTG GAACCATCTG 
GAAGACTTTG ATCAGATCTT CTGGGGCCAG AAGAGTGCCT TGGCCGAGAA GGTTCACCAG 
TGTTGGCAGG AAGATGAACT CTTTGGCTAC CAGTTCCTCA ATGGCGCCAA CCCCATGCTT 
TTGAGACGCT CCACCTCTCT GCCCTCCAGA CTGGTACTGC CCTCTGGGAT GGAGGAGCTT 
CAAGCTCAGT TGGAGAAAGA ACTCAAGAAT GGATCCCTGT TTGAAGCTGA CTTTATCCTG 
CTGGATGGAA TTCCAGCTAA TGTGATCCGA GGAGAACCAC AGTACCTGGC TGCCCCTCTT 
GTCATGCTGA GGATGGACCC CGGTGGGAAG CTGCTACCCA TGGCTATCCA GATTCAGCCC 
CCTAACCCCA GCTCCCCAGC TCCAACACTG TTCCTGCCCT CGGATCCTCC ACTTGCCTGG 
CTCTTGGCTA AGATCTGGGT CCGAAATTCA GATTTCCAAC TGCAGGAGCT CCAATTCCAT 
TTGCTGAACA CCCATCTGGT GGCTGAGGTC ATCGCTGTTG CCACCATGAG ATGCCTCCCA 
GGACTGCACC CTATCTTCAA GCTCCTCGTT CCACACATCC GTTACACTAT GGAAATTAAC 
ACGCGAAGCC GGACCCAGCT CATCTCAGAT GGAGGAATAT TTGATCAGGT AGTGAGCACA 
GGTGGAGGCG GCCATGTTCA GTTGCTTACT CGGGCAGTGG CTCAGCTGAC TTACCACTCC 
CTCTGTCCTC CTGATGATCT GGCTAACCGA GGCCTGCTGA GAATCCCAAG TGCTCTCTAT 
GCTCGGGATG CTTTACAGCT CTGGGAAGTC ACTGCCAGGT ATGTGAAGGG GATGGTCCAT 
CTCTTCTACC AGAGTGATGA TATTGTGAGG GGAGACCCAG AGCTGCAGGC CTGGTGTCGG 
GAGATCACTG AAGTGGGGCT GTGCCATGCC CAGGACAGAG GATTCCCTGT CTCCTTCCAG 
TCCCGGGCTC AGCTCTGCCA TTTCCTCACC ATGTGTGTTT TCACATGCAC TGCCCAGCAT 
GCTGCCATCA ACCAGGGCCA GCTGGACTGG TATGGTTGGG TACCTAATGC TCCATGCACA 
ATGAGGATGC CTCCGCCCAC CAGCAAGGAC GACGTGACGA TGGAGACCGT GATGGGGTCG 
CTACCTGATG TGCAGAAGGC CTGTCTTCAG ATGACTATCA CATGGCACCT GGGTCGCCTG 
CAGCCAGACA TGGTACCTCT AGGACACCAC ACAGAAAAAT ATTTCTCAGA CCCAAGGACC 
AAAGCTGTGC TCAGCCAATT TCAAGCAGAT TTGGACAATT TGGAGAAAGA GATTACAGCC 
CGGAATGAGC AACTTGACCT GCCTTATGAA TACCTCAAGC CCAGCCGCAT AGAGAACAGT 
ATCACCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031466.2
CDS	99..2090
Translation	MGRYRVRVVTGAWLFSGSLNLVRLWLVGEHREAKLELQLRPARGKEEEFDFDVPEDLGPLQFVKLHKQHTVVDDAWFCNLITVQGPGTSAEAVFPCYRWVQGEGILSLPEGTARLAGDNALDVFQKYREKELKERQQTYCWATWKEGLPQTIAADCKDDLPPNMRFHEEKRLDFEWTLKAGVLEMGLKRVYTLLRSWNHLEDFDQIFWGQKSALAEKVHQCWQEDELFGYQFLNGANPMLLRRSTSLPSRLVLPSGMEELQAQLEKELKNGSLFEADFILLDGIPANVIRGEPQYLAAPLVMLRMDPGGKLLPMAIQIQPPNPSSPAPTLFLPSDPPLAWLLAKIWVRNSDFQLQELQFHLLNTHLVAEVIAVATMRCLPGLHPIFKLLVPHIRYTMEINTRSRTQLISDGGIFDQVVSTGGGGHVQLLTRAVAQLTYHSLCPPDDLANRGLLRIPSALYARDALQLWEVTARYVKGMVHLFYQSDDIVRGDPELQAWCREITEVGLCHAQDRGFPVSFQSRAQLCHFLTMCVFTCTAQHAAINQGQLDWYGWVPNAPCTMRMPPPTSKDDVTMETVMGSLPDVQKACLQMTITWHLGRLQPDMVPLGHHTEKYFSDPRTKAVLSQFQADLDNLEKEITARNEQLDLPYEYLKPSRIENSITI

Target ORF information:

RefSeq Version	NM_007440.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus arachidonate 12-lipoxygenase (Alox12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007440.5

ORF Insert Sequence:

ATGGGCCGCT ACCGCGTCCG TGTGGTCACT GGGGCCTGGC TCTTCTCCGG ATCCCTCAAC 
CTAGTGCGTT TGTGGCTGGT TGGGGAGCAC CGGGAGGCGA AACTGGAGCT GCAACTGCGG 
CCAGCGCGGG GCAAGGAGGA GGAGTTTGAC TTCGACGTTC CCGAGGACTT GGGGCCACTG 
CAGTTTGTGA AACTGCACAA ACAGCACACA GTTGTGGATG ACGCCTGGTT CTGCAACCTC 
ATCACAGTTC AGGGGCCGGG GACAAGTGCA GAGGCCGTGT TTCCCTGCTA CCGCTGGGTG 
CAGGGAGAGG GAATCCTGAG CCTCCCGGAG GGGACAGCCC GCCTGGCAGG AGACAATGCC 
TTAGATGTCT TCCAGAAGTA TCGAGAAAAG GAACTGAAGG AAAGACAACA GACCTACTGC 
TGGGCCACCT GGAAAGAAGG CTTACCTCAG ACAATAGCAG CGGACTGTAA GGATGACCTT 
CCTCCAAATA TGAGATTCCA TGAGGAGAAG AGACTGGACT TTGAATGGAC GTTGAAGGCG 
GGGGTTCTGG AAATGGGCCT CAAACGTGTT TATACCCTCC TGAGAAGCTG GAACCATCTG 
GAAGACTTTG ATCAGATCTT CTGGGGCCAG AAGAGTGCCT TGGCCGAGAA GGTTCACCAG 
TGTTGGCAGG AAGATGAACT CTTTGGCTAC CAGTTCCTCA ATGGCGCCAA CCCCATGCTT 
TTGAGACGCT CCACCTCTCT GCCCTCCAGA CTGGTACTGC CCTCTGGGAT GGAGGAGCTT 
CAAGCTCAGT TGGAGAAAGA ACTCAAGAAT GGATCCCTGT TTGAAGCTGA CTTTATCCTG 
CTGGATGGAA TTCCAGCTAA TGTGATCCGA GGAGAACCAC AGTACCTGGC TGCCCCTCTT 
GTCATGCTGA GGATGGACCC CGGTGGGAAG CTGCTACCCA TGGCTATCCA GATTCAGCCC 
CCTAACCCCA GCTCCCCAGC TCCAACACTG TTCCTGCCCT CGGATCCTCC ACTTGCCTGG 
CTCTTGGCTA AGATCTGGGT CCGAAATTCA GATTTCCAAC TGCAGGAGCT CCAATTCCAT 
TTGCTGAACA CCCATCTGGT GGCTGAGGTC ATCGCTGTTG CCACCATGAG ATGCCTCCCA 
GGACTGCACC CTATCTTCAA GCTCCTCGTT CCACACATCC GTTACACTAT GGAAATTAAC 
ACGCGAAGCC GGACCCAGCT CATCTCAGAT GGAGGAATAT TTGATCAGGT AGTGAGCACA 
GGTGGAGGCG GCCATGTTCA GTTGCTTACT CGGGCAGTGG CTCAGCTGAC TTACCACTCC 
CTCTGTCCTC CTGATGATCT GGCTAACCGA GGCCTGCTGA GAATCCCAAG TGCTCTCTAT 
GCTCGGGATG CTTTACAGCT CTGGGAAGTC ACTGCCAGGT ATGTGAAGGG GATGGTCCAT 
CTCTTCTACC AGAGTGATGA TATTGTGAGG GGAGACCCAG AGCTGCAGGC CTGGTGTCGG 
GAGATCACTG AAGTGGGGCT GTGCCATGCC CAGGACAGAG GATTCCCTGT CTCCTTCCAG 
TCCCGGGCTC AGCTCTGCCA TTTCCTCACC ATGTGTGTTT TCACATGCAC TGCCCAGCAT 
GCTGCCATCA ACCAGGGCCA GCTGGACTGG TATGGTTGGG TACCTAATGC TCCATGCACA 
ATGAGGATGC CTCCGCCCAC CAGCAAGGAC GACGTGACGA TGGAGACCGT GATGGGGTCG 
CTACCTGATG TGCAGAAGGC CTGTCTTCAG ATGACTATCA CATGGCACCT GGGTCGCCTG 
CAGCCAGACA TGGTACCTCT AGGACACCAC ACAGAAAAAT ATTTCTCAGA CCCAAGGACC 
AAAGCTGTGC TCAGCCAATT TCAAGCAGAT TTGGACAATT TGGAGAAAGA GATTACAGCC 
CGGAATGAGC AACTTGACCT GCCTTATGAA TACCTCAAGC CCAGCCGCAT AGAGAACAGT 
ATCACCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alox12 ( NM_001331118.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001318047.1 Mus musculus arachidonate 12-lipoxygenase (Alox12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67093
Clone ID Related Accession (Same CDS sequence)	NM_001331118.1 , XM_011248678.1
Accession Version	NM_001331118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	arachidonate 12-lipoxygenase, 12S-type isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL669869.10, AK087724.1 and AK036960.1. On Sep 8, 2016 this sequence version replaced XM_011248677.2. Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream translation start site compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGAGATTCC ATGAGGAGAA GAGACTGGAC TTTGAATGGA CGTTGAAGGC GGGGGTTCTG 
GAAATGGGCC TCAAACGTGT TTATACCCTC CTGAGAAGCT GGAACCATCT GGAAGACTTT 
GATCAGATCT TCTGGGGCCA GAAGAGTGCC TTGGCCGAGA AGGTTCACCA GTGTTGGCAG 
GAAGATGAAC TCTTTGGCTA CCAGTTCCTC AATGGCGCCA ACCCCATGCT TTTGAGACGC 
TCCACCTCTC TGCCCTCCAG ACTGGTACTG CCCTCTGGGA TGGAGGAGCT TCAAGCTCAG 
TTGGAGAAAG AACTCAAGAA TGGATCCCTG TTTGAAGCTG ACTTTATCCT GCTGGATGGA 
ATTCCAGCTA ATGTGATCCG AGGAGAACCA CAGTACCTGG CTGCCCCTCT TGTCATGCTG 
AGGATGGACC CCGGTGGGAA GCTGCTACCC ATGGCTATCC AGATTCAGCC CCCTAACCCC 
AGCTCCCCAG CTCCAACACT GTTCCTGCCC TCGGATCCTC CACTTGCCTG GCTCTTGGCT 
AAGATCTGGG TCCGAAATTC AGATTTCCAA CTGCAGGAGC TCCAATTCCA TTTGCTGAAC 
ACCCATCTGG TGGCTGAGGT CATCGCTGTT GCCACCATGA GATGCCTCCC AGGACTGCAC 
CCTATCTTCA AGCTCCTCGT TCCACACATC CGTTACACTA TGGAAATTAA CACGCGAAGC 
CGGACCCAGC TCATCTCAGA TGGAGGAATA TTTGATCAGG TAGTGAGCAC AGGTGGAGGC 
GGCCATGTTC AGTTGCTTAC TCGGGCAGTG GCTCAGCTGA CTTACCACTC CCTCTGTCCT 
CCTGATGATC TGGCTAACCG AGGCCTGCTG AGAATCCCAA GTGCTCTCTA TGCTCGGGAT 
GCTTTACAGC TCTGGGAAGT CACTGCCAGG TATGTGAAGG GGATGGTCCA TCTCTTCTAC 
CAGAGTGATG ATATTGTGAG GGGAGACCCA GAGCTGCAGG CCTGGTGTCG GGAGATCACT 
GAAGTGGGGC TGTGCCATGC CCAGGACAGA GGATTCCCTG TCTCCTTCCA GTCCCGGGCT 
CAGCTCTGCC ATTTCCTCAC CATGTGTGTT TTCACATGCA CTGCCCAGCA TGCTGCCATC 
AACCAGGGCC AGCTGGACTG GTATGGTTGG GTACCTAATG CTCCATGCAC AATGAGGATG 
CCTCCGCCCA CCAGCAAGGA CGACGTGACG ATGGAGACCG TGATGGGGTC GCTACCTGAT 
GTGCAGAAGG CCTGTCTTCA GATGACTATC ACATGGCACC TGGGTCGCCT GCAGCCAGAC 
ATGGTACCTC TAGGACACCA CACAGAAAAA TATTTCTCAG ACCCAAGGAC CAAAGCTGTG 
CTCAGCCAAT TTCAAGCAGA TTTGGACAAT TTGGAGAAAG AGATTACAGC CCGGAATGAG 
CAACTTGACC TGCCTTATGA ATACCTCAAG CCCAGCCGCA TAGAGAACAG TATCACCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318047.1
CDS	602..2104
Translation	MRFHEEKRLDFEWTLKAGVLEMGLKRVYTLLRSWNHLEDFDQIFWGQKSALAEKVHQCWQEDELFGYQFLNGANPMLLRRSTSLPSRLVLPSGMEELQAQLEKELKNGSLFEADFILLDGIPANVIRGEPQYLAAPLVMLRMDPGGKLLPMAIQIQPPNPSSPAPTLFLPSDPPLAWLLAKIWVRNSDFQLQELQFHLLNTHLVAEVIAVATMRCLPGLHPIFKLLVPHIRYTMEINTRSRTQLISDGGIFDQVVSTGGGGHVQLLTRAVAQLTYHSLCPPDDLANRGLLRIPSALYARDALQLWEVTARYVKGMVHLFYQSDDIVRGDPELQAWCREITEVGLCHAQDRGFPVSFQSRAQLCHFLTMCVFTCTAQHAAINQGQLDWYGWVPNAPCTMRMPPPTSKDDVTMETVMGSLPDVQKACLQMTITWHLGRLQPDMVPLGHHTEKYFSDPRTKAVLSQFQADLDNLEKEITARNEQLDLPYEYLKPSRIENSITI

Target ORF information:

RefSeq Version	NM_001331118.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus arachidonate 12-lipoxygenase (Alox12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331118.1

ORF Insert Sequence:

ATGAGATTCC ATGAGGAGAA GAGACTGGAC TTTGAATGGA CGTTGAAGGC GGGGGTTCTG 
GAAATGGGCC TCAAACGTGT TTATACCCTC CTGAGAAGCT GGAACCATCT GGAAGACTTT 
GATCAGATCT TCTGGGGCCA GAAGAGTGCC TTGGCCGAGA AGGTTCACCA GTGTTGGCAG 
GAAGATGAAC TCTTTGGCTA CCAGTTCCTC AATGGCGCCA ACCCCATGCT TTTGAGACGC 
TCCACCTCTC TGCCCTCCAG ACTGGTACTG CCCTCTGGGA TGGAGGAGCT TCAAGCTCAG 
TTGGAGAAAG AACTCAAGAA TGGATCCCTG TTTGAAGCTG ACTTTATCCT GCTGGATGGA 
ATTCCAGCTA ATGTGATCCG AGGAGAACCA CAGTACCTGG CTGCCCCTCT TGTCATGCTG 
AGGATGGACC CCGGTGGGAA GCTGCTACCC ATGGCTATCC AGATTCAGCC CCCTAACCCC 
AGCTCCCCAG CTCCAACACT GTTCCTGCCC TCGGATCCTC CACTTGCCTG GCTCTTGGCT 
AAGATCTGGG TCCGAAATTC AGATTTCCAA CTGCAGGAGC TCCAATTCCA TTTGCTGAAC 
ACCCATCTGG TGGCTGAGGT CATCGCTGTT GCCACCATGA GATGCCTCCC AGGACTGCAC 
CCTATCTTCA AGCTCCTCGT TCCACACATC CGTTACACTA TGGAAATTAA CACGCGAAGC 
CGGACCCAGC TCATCTCAGA TGGAGGAATA TTTGATCAGG TAGTGAGCAC AGGTGGAGGC 
GGCCATGTTC AGTTGCTTAC TCGGGCAGTG GCTCAGCTGA CTTACCACTC CCTCTGTCCT 
CCTGATGATC TGGCTAACCG AGGCCTGCTG AGAATCCCAA GTGCTCTCTA TGCTCGGGAT 
GCTTTACAGC TCTGGGAAGT CACTGCCAGG TATGTGAAGG GGATGGTCCA TCTCTTCTAC 
CAGAGTGATG ATATTGTGAG GGGAGACCCA GAGCTGCAGG CCTGGTGTCG GGAGATCACT 
GAAGTGGGGC TGTGCCATGC CCAGGACAGA GGATTCCCTG TCTCCTTCCA GTCCCGGGCT 
CAGCTCTGCC ATTTCCTCAC CATGTGTGTT TTCACATGCA CTGCCCAGCA TGCTGCCATC 
AACCAGGGCC AGCTGGACTG GTATGGTTGG GTACCTAATG CTCCATGCAC AATGAGGATG 
CCTCCGCCCA CCAGCAAGGA CGACGTGACG ATGGAGACCG TGATGGGGTC GCTACCTGAT 
GTGCAGAAGG CCTGTCTTCA GATGACTATC ACATGGCACC TGGGTCGCCT GCAGCCAGAC 
ATGGTACCTC TAGGACACCA CACAGAAAAA TATTTCTCAG ACCCAAGGAC CAAAGCTGTG 
CTCAGCCAAT TTCAAGCAGA TTTGGACAAT TTGGAGAAAG AGATTACAGC CCGGAATGAG 
CAACTTGACC TGCCTTATGA ATACCTCAAG CCCAGCCGCA TAGAGAACAG TATCACCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alox12 ( XM_011248678.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246980.1 Mus musculus arachidonate 12-lipoxygenase (Alox12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu67093
Clone ID Related Accession (Same CDS sequence)	NM_001331118.1 , XM_011248678.1
Accession Version	XM_011248678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	arachidonate 12-lipoxygenase, 12S-type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTCC ATGAGGAGAA GAGACTGGAC TTTGAATGGA CGTTGAAGGC GGGGGTTCTG 
GAAATGGGCC TCAAACGTGT TTATACCCTC CTGAGAAGCT GGAACCATCT GGAAGACTTT 
GATCAGATCT TCTGGGGCCA GAAGAGTGCC TTGGCCGAGA AGGTTCACCA GTGTTGGCAG 
GAAGATGAAC TCTTTGGCTA CCAGTTCCTC AATGGCGCCA ACCCCATGCT TTTGAGACGC 
TCCACCTCTC TGCCCTCCAG ACTGGTACTG CCCTCTGGGA TGGAGGAGCT TCAAGCTCAG 
TTGGAGAAAG AACTCAAGAA TGGATCCCTG TTTGAAGCTG ACTTTATCCT GCTGGATGGA 
ATTCCAGCTA ATGTGATCCG AGGAGAACCA CAGTACCTGG CTGCCCCTCT TGTCATGCTG 
AGGATGGACC CCGGTGGGAA GCTGCTACCC ATGGCTATCC AGATTCAGCC CCCTAACCCC 
AGCTCCCCAG CTCCAACACT GTTCCTGCCC TCGGATCCTC CACTTGCCTG GCTCTTGGCT 
AAGATCTGGG TCCGAAATTC AGATTTCCAA CTGCAGGAGC TCCAATTCCA TTTGCTGAAC 
ACCCATCTGG TGGCTGAGGT CATCGCTGTT GCCACCATGA GATGCCTCCC AGGACTGCAC 
CCTATCTTCA AGCTCCTCGT TCCACACATC CGTTACACTA TGGAAATTAA CACGCGAAGC 
CGGACCCAGC TCATCTCAGA TGGAGGAATA TTTGATCAGG TAGTGAGCAC AGGTGGAGGC 
GGCCATGTTC AGTTGCTTAC TCGGGCAGTG GCTCAGCTGA CTTACCACTC CCTCTGTCCT 
CCTGATGATC TGGCTAACCG AGGCCTGCTG AGAATCCCAA GTGCTCTCTA TGCTCGGGAT 
GCTTTACAGC TCTGGGAAGT CACTGCCAGG TATGTGAAGG GGATGGTCCA TCTCTTCTAC 
CAGAGTGATG ATATTGTGAG GGGAGACCCA GAGCTGCAGG CCTGGTGTCG GGAGATCACT 
GAAGTGGGGC TGTGCCATGC CCAGGACAGA GGATTCCCTG TCTCCTTCCA GTCCCGGGCT 
CAGCTCTGCC ATTTCCTCAC CATGTGTGTT TTCACATGCA CTGCCCAGCA TGCTGCCATC 
AACCAGGGCC AGCTGGACTG GTATGGTTGG GTACCTAATG CTCCATGCAC AATGAGGATG 
CCTCCGCCCA CCAGCAAGGA CGACGTGACG ATGGAGACCG TGATGGGGTC GCTACCTGAT 
GTGCAGAAGG CCTGTCTTCA GATGACTATC ACATGGCACC TGGGTCGCCT GCAGCCAGAC 
ATGGTACCTC TAGGACACCA CACAGAAAAA TATTTCTCAG ACCCAAGGAC CAAAGCTGTG 
CTCAGCCAAT TTCAAGCAGA TTTGGACAAT TTGGAGAAAG AGATTACAGC CCGGAATGAG 
CAACTTGACC TGCCTTATGA ATACCTCAAG CCCAGCCGCA TAGAGAACAG TATCACCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246980.1
CDS	175..1677
Translation	MRFHEEKRLDFEWTLKAGVLEMGLKRVYTLLRSWNHLEDFDQIFWGQKSALAEKVHQCWQEDELFGYQFLNGANPMLLRRSTSLPSRLVLPSGMEELQAQLEKELKNGSLFEADFILLDGIPANVIRGEPQYLAAPLVMLRMDPGGKLLPMAIQIQPPNPSSPAPTLFLPSDPPLAWLLAKIWVRNSDFQLQELQFHLLNTHLVAEVIAVATMRCLPGLHPIFKLLVPHIRYTMEINTRSRTQLISDGGIFDQVVSTGGGGHVQLLTRAVAQLTYHSLCPPDDLANRGLLRIPSALYARDALQLWEVTARYVKGMVHLFYQSDDIVRGDPELQAWCREITEVGLCHAQDRGFPVSFQSRAQLCHFLTMCVFTCTAQHAAINQGQLDWYGWVPNAPCTMRMPPPTSKDDVTMETVMGSLPDVQKACLQMTITWHLGRLQPDMVPLGHHTEKYFSDPRTKAVLSQFQADLDNLEKEITARNEQLDLPYEYLKPSRIENSITI

Target ORF information:

RefSeq Version	XM_011248678.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus arachidonate 12-lipoxygenase (Alox12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248678.1

ORF Insert Sequence:

ATGAGATTCC ATGAGGAGAA GAGACTGGAC TTTGAATGGA CGTTGAAGGC GGGGGTTCTG 
GAAATGGGCC TCAAACGTGT TTATACCCTC CTGAGAAGCT GGAACCATCT GGAAGACTTT 
GATCAGATCT TCTGGGGCCA GAAGAGTGCC TTGGCCGAGA AGGTTCACCA GTGTTGGCAG 
GAAGATGAAC TCTTTGGCTA CCAGTTCCTC AATGGCGCCA ACCCCATGCT TTTGAGACGC 
TCCACCTCTC TGCCCTCCAG ACTGGTACTG CCCTCTGGGA TGGAGGAGCT TCAAGCTCAG 
TTGGAGAAAG AACTCAAGAA TGGATCCCTG TTTGAAGCTG ACTTTATCCT GCTGGATGGA 
ATTCCAGCTA ATGTGATCCG AGGAGAACCA CAGTACCTGG CTGCCCCTCT TGTCATGCTG 
AGGATGGACC CCGGTGGGAA GCTGCTACCC ATGGCTATCC AGATTCAGCC CCCTAACCCC 
AGCTCCCCAG CTCCAACACT GTTCCTGCCC TCGGATCCTC CACTTGCCTG GCTCTTGGCT 
AAGATCTGGG TCCGAAATTC AGATTTCCAA CTGCAGGAGC TCCAATTCCA TTTGCTGAAC 
ACCCATCTGG TGGCTGAGGT CATCGCTGTT GCCACCATGA GATGCCTCCC AGGACTGCAC 
CCTATCTTCA AGCTCCTCGT TCCACACATC CGTTACACTA TGGAAATTAA CACGCGAAGC 
CGGACCCAGC TCATCTCAGA TGGAGGAATA TTTGATCAGG TAGTGAGCAC AGGTGGAGGC 
GGCCATGTTC AGTTGCTTAC TCGGGCAGTG GCTCAGCTGA CTTACCACTC CCTCTGTCCT 
CCTGATGATC TGGCTAACCG AGGCCTGCTG AGAATCCCAA GTGCTCTCTA TGCTCGGGAT 
GCTTTACAGC TCTGGGAAGT CACTGCCAGG TATGTGAAGG GGATGGTCCA TCTCTTCTAC 
CAGAGTGATG ATATTGTGAG GGGAGACCCA GAGCTGCAGG CCTGGTGTCG GGAGATCACT 
GAAGTGGGGC TGTGCCATGC CCAGGACAGA GGATTCCCTG TCTCCTTCCA GTCCCGGGCT 
CAGCTCTGCC ATTTCCTCAC CATGTGTGTT TTCACATGCA CTGCCCAGCA TGCTGCCATC 
AACCAGGGCC AGCTGGACTG GTATGGTTGG GTACCTAATG CTCCATGCAC AATGAGGATG 
CCTCCGCCCA CCAGCAAGGA CGACGTGACG ATGGAGACCG TGATGGGGTC GCTACCTGAT 
GTGCAGAAGG CCTGTCTTCA GATGACTATC ACATGGCACC TGGGTCGCCT GCAGCCAGAC 
ATGGTACCTC TAGGACACCA CACAGAAAAA TATTTCTCAG ACCCAAGGAC CAAAGCTGTG 
CTCAGCCAAT TTCAAGCAGA TTTGGACAAT TTGGAGAAAG AGATTACAGC CCGGAATGAG 
CAACTTGACC TGCCTTATGA ATACCTCAAG CCCAGCCGCA TAGAGAACAG TATCACCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An ALOX12-12-HETE-GPR31 signaling axis is a key mediator of hepatic ischemia-reperfusion injury.
Nature medicine24(1)73-83(2018 Jan)
Zhang XJ,Cheng X,Yan ZZ,Fang J,Wang X,Wang W,Liu ZY,Shen LJ,Zhang P,Wang PX,Liao R,Ji YX,Wang JY,Tian S,Zhu XY,Zhang Y,Tian RF,Wang L,Ma XL,Huang Z,She ZG,Li H

Networks of enzymatically oxidized membrane lipids support calcium-dependent coagulation factor binding to maintain hemostasis.
Science signaling10(507)(2017 Nov)
Lauder SN,Allen-Redpath K,Slatter DA,Aldrovandi M,O'Connor A,Farewell D,Percy CL,Molhoek JE,Rannikko S,Tyrrell VJ,Ferla S,Milne GL,Poole AW,Thomas CP,Obaji S,Taylor PR,Jones SA,de Groot PG,Urbanus RT,H?rkk? S,Uderhardt S,Ackermann J,Vince Jenkins P,Brancale A,Kr?nke G,Collins PW,O'Donnell VB

12(S)-HETrE, a 12-Lipoxygenase Oxylipin of Dihomo-?-Linolenic Acid, Inhibits Thrombosis via G?s Signaling in Platelets.
Arteriosclerosis, thrombosis, and vascular biology36(10)2068-77(2016 Oct)
Yeung J,Tourdot BE,Adili R,Green AR,Freedman CJ,Fernandez-Perez P,Yu J,Holman TR,Holinstat M

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
PLoS genetics12(9)e1006355(2016 Sep)
Wu Y,Wei FY,Kawarada L,Suzuki T,Araki K,Komohara Y,Fujimura A,Kaitsuka T,Takeya M,Oike Y,Suzuki T,Tomizawa K

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Alox12 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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