Alas2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alas2
Entrez Gene ID	11656
Full Name	aminolevulinic acid synthase 2, erythroid
Synonyms	ALAS,ALAS-E,ALASE,Alas-2
General protein information	Preferred Names aminolevulinic acid synthase 2, erythroid Names 5-aminolevulinate synthase, erythroid-specific, mitochondrial 5-aminolevulinic acid synthase 2 delta-ALA synthase 2 delta-aminolevulinate synthase 2 erythroid-specific ALAS
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F3\|X 68.46 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009653.3	NP_033783.1	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform a
XM_030251199.1	XP_030107059.1	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform X1
NM_001102446.1	NP_001095916.1	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform b

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Alas	NP_477281.1
Xenopus tropicalis (tropical clawed frog)	alas2	NP_001006926.1
Canis lupus familiaris (dog)	ALAS2	XP_548619.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003184	XP_312882.5
Saccharomyces cerevisiae (baker's yeast)	HEM1	NP_010518.1
Eremothecium gossypii	AGOS_ABL104C	NP_982843.1
Danio rerio (zebrafish)	alas2	NP_571757.1
Magnaporthe oryzae (rice blast fungus)	MGG_06446	XP_003717121.1
Bos taurus (cattle)	ALAS2	NP_001030275.1
Rattus norvegicus (Norway rat)	Alas2	NP_037329.1
Schizosaccharomyces pombe (fission yeast)	hem1	NP_594388.1
Neurospora crassa	NCU06189	XP_963001.2
Homo sapiens (human)	ALAS2	NP_000023.2
Pan troglodytes (chimpanzee)	ALAS2	XP_528998.2
	ALAS2	XP_002806298.1
Mus musculus (house mouse)	Alas2	NP_033783.1
Kluyveromyces lactis	KLLA0C15059g	XP_452875.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular dynamics analysis of the structural and dynamic properties of the functionally enhanced hepta-variant of mouse 5-aminolevulinate synthase.
Na I, DeForte S, Stojanovski BM, Ferreira GC, Uversky VN
Journal of biomolecular structure & dynamics36(1)152-165(2018 Jan)

Intron 1 GATA site enhances ALAS2 expression indispensably during erythroid differentiation.
Zhang Y, Zhang J, An W, Wan Y, Ma S, Yin J, Li X, Gao J, Yuan W, Guo Y, Engel JD, Shi L, Cheng T, Zhu X
Nucleic acids research45(2)657-671(2017 Jan)

Murine erythroid 5-aminolevulinate synthase: Truncation of a disordered N-terminal extension is not detrimental for catalysis.
Stojanovski BM, Breydo L, Uversky VN, Ferreira GC
Biochimica et biophysica acta1864(5)441-52(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We propose that the N-terminal truncation offers a cell-specific ALAS2 regulatory mechanism without hindering heme synthesis
Title: Murine erythroid 5-aminolevulinate synthase: Truncation of a disordered N-terminal extension is not detrimental for catalysis.

N150F ALAS variant catalyzes the forward reaction at a mere 1.2-fold faster rate than that of the reverse reaction.
Title: Asn-150 of Murine Erythroid 5-Aminolevulinate Synthase Modulates the Catalytic Balance between the Rates of the Reversible Reaction.

The rate of ALA release is also controlled by a hysteretic kinetic mechanism (observed as a lag in the ALA external aldimine formation progress curve), consistent with conformational changes governing the dissociation of ALA from ALAS.
Title: Unstable reaction intermediates and hysteresis during the catalytic cycle of 5-aminolevulinate synthase: implications from using pseudo and alternate substrates and a promiscuous enzyme variant.

impaired mitochondrial [Fe-S] cluster biogenesis in Mfrn1(gt/gt) cells results in elevated IRP1 RNA-binding that attenuates ALAS2 mRNA translation and protoporphyrin accumulation
Title: Iron regulatory protein-1 protects against mitoferrin-1-deficient porphyria.

Light treatments revealed that ALAS2 expression results in an increase in cell death in comparison to aminolevulinic acid (ALA) treatment producing a similar amount of protoprophyrin IX.
Title: Expression of murine 5-aminolevulinate synthase variants causes protoporphyrin IX accumulation and light-induced mammalian cell death.

The following Alas2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alas2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21979	NM_009653.3 Latest version!	Mus musculus aminolevulinic acid synthase 2, erythroid (Alas2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
OMu22087	NM_001102446.1 Latest version!	Mus musculus aminolevulinic acid synthase 2, erythroid (Alas2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$335.30 $479.00
OMu90356	XM_030251199.1 Latest version!	Mus musculus aminolevulinic acid synthase 2, erythroid (Alas2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alas2 ( NM_009653.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033783.1 Mus musculus aminolevulinic acid synthase 2, erythroid (Alas2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu21979
Clone ID Related Accession (Same CDS sequence)	NM_009653.3
Accession Version	NM_009653.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY035435.1, AK077610.1, AK002642.1 and M15268.1. On Aug 24, 2007 this sequence version replaced NM_009653.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK077610.1, AK002642.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGGCAG CAGCTATGTT GCTACGGTCC TGTCCAGTGC TCTCTCAGGG CCCCACAGGC 
CTCCTGGGCA AAGTGGCTAA AACCTACCAG TTCCTATTTA GTATTGGACG CTGCCCCATC 
CTGGCCACTC AAGGACCAAC CTGTTCTCAA ATCCATCTTA AGGCAACCAA GGCTGGAGGA 
GACTCTCCAT CTTGGGCTAA GAGCCATTGT CCTTTCATGC TGTCAGAACT CCAAGACAGG 
AAGAGCAAGA TTGTGCAGAG GGCAGCTCCA GAAGTTCAAG AGGATGTCAA GACTTTCAAG 
ACAGACCTGC TGAGCACCAT GGATTCAACC ACCCGAAGCC ATTCATTTCC TAGTTTCCAG 
GAGCCAGAGC AGACTGAAGG GGCAGTTCCC CACCTGATTC AGAACAATAT GACTGGAAGC 
CAGGCTTTCG GTTATGACCA ATTTTTCAGA GACAAGATCA TGGAGAAGAA ACAGGACCAC 
ACCTACCGTG TGTTCAAGAC TGTGAATCGT TGGGCTAATG CCTACCCCTT TGCCCAACAC 
TTCTCCGAGG CATCTATGGC ATCAAAGGAT GTTTCTGTTT GGTGTAGTAA TGACTATTTG 
GGCATAAGCA GACACCCTCG TGTCTTGCAG GCCATAGAGG AGACCCTGAA GAATCATGGA 
GCTGGAGCTG GGGGCACTCG CAATATCTCA GGTACCAGCA AGTTTCATGT GGAGCTTGAA 
CAGGAGCTGG CTGAACTACA CCAGAAAGAC TCAGCTCTGC TCTTCTCCTC CTGTTTTGTG 
GCCAATGATT CTACTCTCTT TACACTGGCC AAGCTTCTGC CAGGGTGTGA GATCTACTCA 
GATGCAGGCA ATCATGCCTC CATGATCCAA GGCATTCGCA ACAGTGGTGC AGCCAAGTTT 
GTCTTCAGAC ACAATGACCC AGGCCACCTG AAGAAACTTC TCGAGAAGTC TGATCCCAAG 
ACACCAAAAA TTGTGGCTTT TGAGACTGTT CATTCCATGG ATGGTGCCAT CTGTCCTCTG 
GAGGAATTGT GTGATGTGGC CCACCAGTAT GGAGCCCTGA CCTTCGTAGA TGAAGTCCAT 
GCTGTAGGAC TGTATGGAGC CCGGGGTGCA GGTATCGGGG AGCGTGATGG AATTATGCAC 
AAGCTTGACA TCATCTCTGG AACTCTTGGC AAGGCCTTTG GTTGCGTCGG TGGCTATATA 
GCCAGCACTC GGGACTTGGT GGACATGGTG CGCTCCTACG CTGCAGGCTT CATCTTTACC 
ACTTCACTGC CTCCCATGGT GCTCTCTGGG GCTCTAGAAT CTGTGCGCCT ACTCAAGGGA 
GAGGAGGGTC AAGCCCTGAG GCGGGCACAC CAGCGCAATG TCAAACACAT GCGCCAGCTG 
CTAATGGACA GGGGCTTTCC TGTTATCCCC TGTCCCAGCC ACATCATCCC CATCAGGGTG 
GGTAATGCAG CACTCAACAG CAAGATCTGT GATCTTCTGC TCTCCAAGCA CAGCATCTAT 
GTGCAGGCCA TCAACTACCC AACTGTGCCT CGTGGTGAGG AGCTACTGCG CTTGGCCCCC 
TCCCCCCACC ACAGCCCTCA GATGATGGAA AACTTTGTGG AGAAGCTGCT GCTGGCCTGG 
ACTGAGGTGG GGCTGCCCCT CCAAGATGTG TCTGTGGCTG CATGCAACTT CTGTCATCGT 
CCTGTGCACT TTGAACTTAT GAGCGAGTGG GAGCGATCCT ACTTTGGGAA CATGGGACCC 
CAATATGTTA CCACCTATGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033783.1
CDS	85..1848
Translation	MVAAAMLLRSCPVLSQGPTGLLGKVAKTYQFLFSIGRCPILATQGPTCSQIHLKATKAGGDSPSWAKSHCPFMLSELQDRKSKIVQRAAPEVQEDVKTFKTDLLSTMDSTTRSHSFPSFQEPEQTEGAVPHLIQNNMTGSQAFGYDQFFRDKIMEKKQDHTYRVFKTVNRWANAYPFAQHFSEASMASKDVSVWCSNDYLGISRHPRVLQAIEETLKNHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKLLPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPGHLKKLLEKSDPKTPKIVAFETVHSMDGAICPLEELCDVAHQYGALTFVDEVHAVGLYGARGAGIGERDGIMHKLDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGFPVIPCPSHIIPIRVGNAALNSKICDLLLSKHSIYVQAINYPTVPRGEELLRLAPSPHHSPQMMENFVEKLLLAWTEVGLPLQDVSVAACNFCHRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	NM_009653.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus aminolevulinic acid synthase 2, erythroid (Alas2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009653.3

ORF Insert Sequence:

ATGGTGGCAG CAGCTATGTT GCTACGGTCC TGTCCAGTGC TCTCTCAGGG CCCCACAGGC 
CTCCTGGGCA AAGTGGCTAA AACCTACCAG TTCCTATTTA GTATTGGACG CTGCCCCATC 
CTGGCCACTC AAGGACCAAC CTGTTCTCAA ATCCATCTTA AGGCAACCAA GGCTGGAGGA 
GACTCTCCAT CTTGGGCTAA GAGCCATTGT CCTTTCATGC TGTCAGAACT CCAAGACAGG 
AAGAGCAAGA TTGTGCAGAG GGCAGCTCCA GAAGTTCAAG AGGATGTCAA GACTTTCAAG 
ACAGACCTGC TGAGCACCAT GGATTCAACC ACCCGAAGCC ATTCATTTCC TAGTTTCCAG 
GAGCCAGAGC AGACTGAAGG GGCAGTTCCC CACCTGATTC AGAACAATAT GACTGGAAGC 
CAGGCTTTCG GTTATGACCA ATTTTTCAGA GACAAGATCA TGGAGAAGAA ACAGGACCAC 
ACCTACCGTG TGTTCAAGAC TGTGAATCGT TGGGCTAATG CCTACCCCTT TGCCCAACAC 
TTCTCCGAGG CATCTATGGC ATCAAAGGAT GTTTCTGTTT GGTGTAGTAA TGACTATTTG 
GGCATAAGCA GACACCCTCG TGTCTTGCAG GCCATAGAGG AGACCCTGAA GAATCATGGA 
GCTGGAGCTG GGGGCACTCG CAATATCTCA GGTACCAGCA AGTTTCATGT GGAGCTTGAA 
CAGGAGCTGG CTGAACTACA CCAGAAAGAC TCAGCTCTGC TCTTCTCCTC CTGTTTTGTG 
GCCAATGATT CTACTCTCTT TACACTGGCC AAGCTTCTGC CAGGGTGTGA GATCTACTCA 
GATGCAGGCA ATCATGCCTC CATGATCCAA GGCATTCGCA ACAGTGGTGC AGCCAAGTTT 
GTCTTCAGAC ACAATGACCC AGGCCACCTG AAGAAACTTC TCGAGAAGTC TGATCCCAAG 
ACACCAAAAA TTGTGGCTTT TGAGACTGTT CATTCCATGG ATGGTGCCAT CTGTCCTCTG 
GAGGAATTGT GTGATGTGGC CCACCAGTAT GGAGCCCTGA CCTTCGTAGA TGAAGTCCAT 
GCTGTAGGAC TGTATGGAGC CCGGGGTGCA GGTATCGGGG AGCGTGATGG AATTATGCAC 
AAGCTTGACA TCATCTCTGG AACTCTTGGC AAGGCCTTTG GTTGCGTCGG TGGCTATATA 
GCCAGCACTC GGGACTTGGT GGACATGGTG CGCTCCTACG CTGCAGGCTT CATCTTTACC 
ACTTCACTGC CTCCCATGGT GCTCTCTGGG GCTCTAGAAT CTGTGCGCCT ACTCAAGGGA 
GAGGAGGGTC AAGCCCTGAG GCGGGCACAC CAGCGCAATG TCAAACACAT GCGCCAGCTG 
CTAATGGACA GGGGCTTTCC TGTTATCCCC TGTCCCAGCC ACATCATCCC CATCAGGGTG 
GGTAATGCAG CACTCAACAG CAAGATCTGT GATCTTCTGC TCTCCAAGCA CAGCATCTAT 
GTGCAGGCCA TCAACTACCC AACTGTGCCT CGTGGTGAGG AGCTACTGCG CTTGGCCCCC 
TCCCCCCACC ACAGCCCTCA GATGATGGAA AACTTTGTGG AGAAGCTGCT GCTGGCCTGG 
ACTGAGGTGG GGCTGCCCCT CCAAGATGTG TCTGTGGCTG CATGCAACTT CTGTCATCGT 
CCTGTGCACT TTGAACTTAT GAGCGAGTGG GAGCGATCCT ACTTTGGGAA CATGGGACCC 
CAATATGTTA CCACCTATGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alas2 ( NM_001102446.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001095916.1 Mus musculus aminolevulinic acid synthase 2, erythroid (Alas2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22087
Clone ID Related Accession (Same CDS sequence)	NM_001102446.1
Accession Version	NM_001102446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY035435.1, M15268.1 and AK002642.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the end of an exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M15268.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGGCAG CAGCTATGTT GCTACGGTCC TGTCCAGTGC TCTCTCAGGG CCCCACAGGC 
CTCCTGGGCA AAGTGGCTAA AACCTACCAG TTCCTATTTA GTATTGGACG CTGCCCCATC 
CTGGCCACTC AAGGACCAAC CTGTTCTCAA ATCCATCTTA AGGCAACCAA GGCTGGAGGA 
GAACTCCAAG ACAGGAAGAG CAAGATTGTG CAGAGGGCAG CTCCAGAAGT TCAAGAGGAT 
GTCAAGACTT TCAAGACAGA CCTGCTGAGC ACCATGGATT CAACCACCCG AAGCCATTCA 
TTTCCTAGTT TCCAGGAGCC AGAGCAGACT GAAGGGGCAG TTCCCCACCT GATTCAGAAC 
AATATGACTG GAAGCCAGGC TTTCGGTTAT GACCAATTTT TCAGAGACAA GATCATGGAG 
AAGAAACAGG ACCACACCTA CCGTGTGTTC AAGACTGTGA ATCGTTGGGC TAATGCCTAC 
CCCTTTGCCC AACACTTCTC CGAGGCATCT ATGGCATCAA AGGATGTTTC TGTTTGGTGT 
AGTAATGACT ATTTGGGCAT AAGCAGACAC CCTCGTGTCT TGCAGGCCAT AGAGGAGACC 
CTGAAGAATC ATGGAGCTGG AGCTGGGGGC ACTCGCAATA TCTCAGGTAC CAGCAAGTTT 
CATGTGGAGC TTGAACAGGA GCTGGCTGAA CTACACCAGA AAGACTCAGC TCTGCTCTTC 
TCCTCCTGTT TTGTGGCCAA TGATTCTACT CTCTTTACAC TGGCCAAGCT TCTGCCAGGG 
TGTGAGATCT ACTCAGATGC AGGCAATCAT GCCTCCATGA TCCAAGGCAT TCGCAACAGT 
GGTGCAGCCA AGTTTGTCTT CAGACACAAT GACCCAGGCC ACCTGAAGAA ACTTCTCGAG 
AAGTCTGATC CCAAGACACC AAAAATTGTG GCTTTTGAGA CTGTTCATTC CATGGATGGT 
GCCATCTGTC CTCTGGAGGA ATTGTGTGAT GTGGCCCACC AGTATGGAGC CCTGACCTTC 
GTAGATGAAG TCCATGCTGT AGGACTGTAT GGAGCCCGGG GTGCAGGTAT CGGGGAGCGT 
GATGGAATTA TGCACAAGCT TGACATCATC TCTGGAACTC TTGGCAAGGC CTTTGGTTGC 
GTCGGTGGCT ATATAGCCAG CACTCGGGAC TTGGTGGACA TGGTGCGCTC CTACGCTGCA 
GGCTTCATCT TTACCACTTC ACTGCCTCCC ATGGTGCTCT CTGGGGCTCT AGAATCTGTG 
CGCCTACTCA AGGGAGAGGA GGGTCAAGCC CTGAGGCGGG CACACCAGCG CAATGTCAAA 
CACATGCGCC AGCTGCTAAT GGACAGGGGC TTTCCTGTTA TCCCCTGTCC CAGCCACATC 
ATCCCCATCA GGGTGGGTAA TGCAGCACTC AACAGCAAGA TCTGTGATCT TCTGCTCTCC 
AAGCACAGCA TCTATGTGCA GGCCATCAAC TACCCAACTG TGCCTCGTGG TGAGGAGCTA 
CTGCGCTTGG CCCCCTCCCC CCACCACAGC CCTCAGATGA TGGAAAACTT TGTGGAGAAG 
CTGCTGCTGG CCTGGACTGA GGTGGGGCTG CCCCTCCAAG ATGTGTCTGT GGCTGCATGC 
AACTTCTGTC ATCGTCCTGT GCACTTTGAA CTTATGAGCG AGTGGGAGCG ATCCTACTTT 
GGGAACATGG GACCCCAATA TGTTACCACC TATGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095916.1
CDS	85..1803
Translation	MVAAAMLLRSCPVLSQGPTGLLGKVAKTYQFLFSIGRCPILATQGPTCSQIHLKATKAGGELQDRKSKIVQRAAPEVQEDVKTFKTDLLSTMDSTTRSHSFPSFQEPEQTEGAVPHLIQNNMTGSQAFGYDQFFRDKIMEKKQDHTYRVFKTVNRWANAYPFAQHFSEASMASKDVSVWCSNDYLGISRHPRVLQAIEETLKNHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKLLPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPGHLKKLLEKSDPKTPKIVAFETVHSMDGAICPLEELCDVAHQYGALTFVDEVHAVGLYGARGAGIGERDGIMHKLDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGFPVIPCPSHIIPIRVGNAALNSKICDLLLSKHSIYVQAINYPTVPRGEELLRLAPSPHHSPQMMENFVEKLLLAWTEVGLPLQDVSVAACNFCHRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	NM_001102446.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus aminolevulinic acid synthase 2, erythroid (Alas2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102446.1

ORF Insert Sequence:

ATGGTGGCAG CAGCTATGTT GCTACGGTCC TGTCCAGTGC TCTCTCAGGG CCCCACAGGC 
CTCCTGGGCA AAGTGGCTAA AACCTACCAG TTCCTATTTA GTATTGGACG CTGCCCCATC 
CTGGCCACTC AAGGACCAAC CTGTTCTCAA ATCCATCTTA AGGCAACCAA GGCTGGAGGA 
GAACTCCAAG ACAGGAAGAG CAAGATTGTG CAGAGGGCAG CTCCAGAAGT TCAAGAGGAT 
GTCAAGACTT TCAAGACAGA CCTGCTGAGC ACCATGGATT CAACCACCCG AAGCCATTCA 
TTTCCTAGTT TCCAGGAGCC AGAGCAGACT GAAGGGGCAG TTCCCCACCT GATTCAGAAC 
AATATGACTG GAAGCCAGGC TTTCGGTTAT GACCAATTTT TCAGAGACAA GATCATGGAG 
AAGAAACAGG ACCACACCTA CCGTGTGTTC AAGACTGTGA ATCGTTGGGC TAATGCCTAC 
CCCTTTGCCC AACACTTCTC CGAGGCATCT ATGGCATCAA AGGATGTTTC TGTTTGGTGT 
AGTAATGACT ATTTGGGCAT AAGCAGACAC CCTCGTGTCT TGCAGGCCAT AGAGGAGACC 
CTGAAGAATC ATGGAGCTGG AGCTGGGGGC ACTCGCAATA TCTCAGGTAC CAGCAAGTTT 
CATGTGGAGC TTGAACAGGA GCTGGCTGAA CTACACCAGA AAGACTCAGC TCTGCTCTTC 
TCCTCCTGTT TTGTGGCCAA TGATTCTACT CTCTTTACAC TGGCCAAGCT TCTGCCAGGG 
TGTGAGATCT ACTCAGATGC AGGCAATCAT GCCTCCATGA TCCAAGGCAT TCGCAACAGT 
GGTGCAGCCA AGTTTGTCTT CAGACACAAT GACCCAGGCC ACCTGAAGAA ACTTCTCGAG 
AAGTCTGATC CCAAGACACC AAAAATTGTG GCTTTTGAGA CTGTTCATTC CATGGATGGT 
GCCATCTGTC CTCTGGAGGA ATTGTGTGAT GTGGCCCACC AGTATGGAGC CCTGACCTTC 
GTAGATGAAG TCCATGCTGT AGGACTGTAT GGAGCCCGGG GTGCAGGTAT CGGGGAGCGT 
GATGGAATTA TGCACAAGCT TGACATCATC TCTGGAACTC TTGGCAAGGC CTTTGGTTGC 
GTCGGTGGCT ATATAGCCAG CACTCGGGAC TTGGTGGACA TGGTGCGCTC CTACGCTGCA 
GGCTTCATCT TTACCACTTC ACTGCCTCCC ATGGTGCTCT CTGGGGCTCT AGAATCTGTG 
CGCCTACTCA AGGGAGAGGA GGGTCAAGCC CTGAGGCGGG CACACCAGCG CAATGTCAAA 
CACATGCGCC AGCTGCTAAT GGACAGGGGC TTTCCTGTTA TCCCCTGTCC CAGCCACATC 
ATCCCCATCA GGGTGGGTAA TGCAGCACTC AACAGCAAGA TCTGTGATCT TCTGCTCTCC 
AAGCACAGCA TCTATGTGCA GGCCATCAAC TACCCAACTG TGCCTCGTGG TGAGGAGCTA 
CTGCGCTTGG CCCCCTCCCC CCACCACAGC CCTCAGATGA TGGAAAACTT TGTGGAGAAG 
CTGCTGCTGG CCTGGACTGA GGTGGGGCTG CCCCTCCAAG ATGTGTCTGT GGCTGCATGC 
AACTTCTGTC ATCGTCCTGT GCACTTTGAA CTTATGAGCG AGTGGGAGCG ATCCTACTTT 
GGGAACATGG GACCCCAATA TGTTACCACC TATGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alas2 ( XM_030251199.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107059.1 Mus musculus aminolevulinic acid synthase 2, erythroid (Alas2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu90356
Clone ID Related Accession (Same CDS sequence)	XM_030251199.1
Accession Version	XM_030251199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACA GCCCCACAAA AATGGACTTT GGGCTCAGGA TGGTGGCAGC AGCTATGTTG 
CTACGGTCCT GTCCAGTGCT CTCTCAGGGC CCCACAGGCC TCCTGGGCAA AGTGGCTAAA 
ACCTACCAGT TCCTATTTAG TATTGGACGC TGCCCCATCC TGGCCACTCA AGGACCAACC 
TGTTCTCAAA TCCATCTTAA GGCAACCAAG GCTGGAGGAG ACTCTCCATC TTGGGCTAAG 
AGCCATTGTC CTTTCATGCT GTCAGAACTC CAAGACAGGA AGAGCAAGAT TGTGCAGAGG 
GCAGCTCCAG AAGTTCAAGA GGATGTCAAG ACTTTCAAGA CAGACCTGCT GAGCACCATG 
GATTCAACCA CCCGAAGCCA TTCATTTCCT AGTTTCCAGG AGCCAGAGCA GACTGAAGGG 
GCAGTTCCCC ACCTGATTCA GAACAATATG ACTGGAAGCC AGGCTTTCGG TTATGACCAA 
TTTTTCAGAG ACAAGATCAT GGAGAAGAAA CAGGACCACA CCTACCGTGT GTTCAAGACT 
GTGAATCGTT GGGCTAATGC CTACCCCTTT GCCCAACACT TCTCCGAGGC ATCTATGGCA 
TCAAAGGATG TTTCTGTTTG GTGTAGTAAT GACTATTTGG GCATAAGCAG ACACCCTCGT 
GTCTTGCAGG CCATAGAGGA GACCCTGAAG AATCATGGAG CTGGAGCTGG GGGCACTCGC 
AATATCTCAG GTACCAGCAA GTTTCATGTG GAGCTTGAAC AGGAGCTGGC TGAACTACAC 
CAGAAAGACT CAGCTCTGCT CTTCTCCTCC TGTTTTGTGG CCAATGATTC TACTCTCTTT 
ACACTGGCCA AGCTTCTGCC AGGGTGTGAG ATCTACTCAG ATGCAGGCAA TCATGCCTCC 
ATGATCCAAG GCATTCGCAA CAGTGGTGCA GCCAAGTTTG TCTTCAGACA CAATGACCCA 
GGCCACCTGA AGAAACTTCT CGAGAAGTCT GATCCCAAGA CACCAAAAAT TGTGGCTTTT 
GAGACTGTTC ATTCCATGGA TGGTGCCATC TGTCCTCTGG AGGAATTGTG TGATGTGGCC 
CACCAGTATG GAGCCCTGAC CTTCGTAGAT GAAGTCCATG CTGTAGGACT GTATGGAGCC 
CGGGGTGCAG GTATCGGGGA GCGTGATGGA ATTATGCACA AGCTTGACAT CATCTCTGGA 
ACTCTTGGCA AGGCCTTTGG TTGCGTCGGT GGCTATATAG CCAGCACTCG GGACTTGGTG 
GACATGGTGC GCTCCTACGC TGCAGGCTTC ATCTTTACCA CTTCACTGCC TCCCATGGTG 
CTCTCTGGGG CTCTAGAATC TGTGCGCCTA CTCAAGGGAG AGGAGGGTCA AGCCCTGAGG 
CGGGCACACC AGCGCAATGT CAAACACATG CGCCAGCTGC TAATGGACAG GGGCTTTCCT 
GTTATCCCCT GTCCCAGCCA CATCATCCCC ATCAGGGTGG GTAATGCAGC ACTCAACAGC 
AAGATCTGTG ATCTTCTGCT CTCCAAGCAC AGCATCTATG TGCAGGCCAT CAACTACCCA 
ACTGTGCCTC GTGGTGAGGA GCTACTGCGC TTGGCCCCCT CCCCCCACCA CAGCCCTCAG 
ATGATGGAAA ACTTTGTGGA GAAGCTGCTG CTGGCCTGGA CTGAGGTGGG GCTGCCCCTC 
CAAGATGTGT CTGTGGCTGC ATGCAACTTC TGTCATCGTC CTGTGCACTT TGAACTTATG 
AGCGAGTGGG AGCGATCCTA CTTTGGGAAC ATGGGACCCC AATATGTTAC CACCTATGCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107059.1
CDS	50..1852
Translation	MANSPTKMDFGLRMVAAAMLLRSCPVLSQGPTGLLGKVAKTYQFLFSIGRCPILATQGPTCSQIHLKATKAGGDSPSWAKSHCPFMLSELQDRKSKIVQRAAPEVQEDVKTFKTDLLSTMDSTTRSHSFPSFQEPEQTEGAVPHLIQNNMTGSQAFGYDQFFRDKIMEKKQDHTYRVFKTVNRWANAYPFAQHFSEASMASKDVSVWCSNDYLGISRHPRVLQAIEETLKNHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKLLPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPGHLKKLLEKSDPKTPKIVAFETVHSMDGAICPLEELCDVAHQYGALTFVDEVHAVGLYGARGAGIGERDGIMHKLDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGFPVIPCPSHIIPIRVGNAALNSKICDLLLSKHSIYVQAINYPTVPRGEELLRLAPSPHHSPQMMENFVEKLLLAWTEVGLPLQDVSVAACNFCHRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	XM_030251199.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus aminolevulinic acid synthase 2, erythroid (Alas2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251199.1

ORF Insert Sequence:

ATGGCCAACA GCCCCACAAA AATGGACTTT GGGCTCAGGA TGGTGGCAGC AGCTATGTTG 
CTACGGTCCT GTCCAGTGCT CTCTCAGGGC CCCACAGGCC TCCTGGGCAA AGTGGCTAAA 
ACCTACCAGT TCCTATTTAG TATTGGACGC TGCCCCATCC TGGCCACTCA AGGACCAACC 
TGTTCTCAAA TCCATCTTAA GGCAACCAAG GCTGGAGGAG ACTCTCCATC TTGGGCTAAG 
AGCCATTGTC CTTTCATGCT GTCAGAACTC CAAGACAGGA AGAGCAAGAT TGTGCAGAGG 
GCAGCTCCAG AAGTTCAAGA GGATGTCAAG ACTTTCAAGA CAGACCTGCT GAGCACCATG 
GATTCAACCA CCCGAAGCCA TTCATTTCCT AGTTTCCAGG AGCCAGAGCA GACTGAAGGG 
GCAGTTCCCC ACCTGATTCA GAACAATATG ACTGGAAGCC AGGCTTTCGG TTATGACCAA 
TTTTTCAGAG ACAAGATCAT GGAGAAGAAA CAGGACCACA CCTACCGTGT GTTCAAGACT 
GTGAATCGTT GGGCTAATGC CTACCCCTTT GCCCAACACT TCTCCGAGGC ATCTATGGCA 
TCAAAGGATG TTTCTGTTTG GTGTAGTAAT GACTATTTGG GCATAAGCAG ACACCCTCGT 
GTCTTGCAGG CCATAGAGGA GACCCTGAAG AATCATGGAG CTGGAGCTGG GGGCACTCGC 
AATATCTCAG GTACCAGCAA GTTTCATGTG GAGCTTGAAC AGGAGCTGGC TGAACTACAC 
CAGAAAGACT CAGCTCTGCT CTTCTCCTCC TGTTTTGTGG CCAATGATTC TACTCTCTTT 
ACACTGGCCA AGCTTCTGCC AGGGTGTGAG ATCTACTCAG ATGCAGGCAA TCATGCCTCC 
ATGATCCAAG GCATTCGCAA CAGTGGTGCA GCCAAGTTTG TCTTCAGACA CAATGACCCA 
GGCCACCTGA AGAAACTTCT CGAGAAGTCT GATCCCAAGA CACCAAAAAT TGTGGCTTTT 
GAGACTGTTC ATTCCATGGA TGGTGCCATC TGTCCTCTGG AGGAATTGTG TGATGTGGCC 
CACCAGTATG GAGCCCTGAC CTTCGTAGAT GAAGTCCATG CTGTAGGACT GTATGGAGCC 
CGGGGTGCAG GTATCGGGGA GCGTGATGGA ATTATGCACA AGCTTGACAT CATCTCTGGA 
ACTCTTGGCA AGGCCTTTGG TTGCGTCGGT GGCTATATAG CCAGCACTCG GGACTTGGTG 
GACATGGTGC GCTCCTACGC TGCAGGCTTC ATCTTTACCA CTTCACTGCC TCCCATGGTG 
CTCTCTGGGG CTCTAGAATC TGTGCGCCTA CTCAAGGGAG AGGAGGGTCA AGCCCTGAGG 
CGGGCACACC AGCGCAATGT CAAACACATG CGCCAGCTGC TAATGGACAG GGGCTTTCCT 
GTTATCCCCT GTCCCAGCCA CATCATCCCC ATCAGGGTGG GTAATGCAGC ACTCAACAGC 
AAGATCTGTG ATCTTCTGCT CTCCAAGCAC AGCATCTATG TGCAGGCCAT CAACTACCCA 
ACTGTGCCTC GTGGTGAGGA GCTACTGCGC TTGGCCCCCT CCCCCCACCA CAGCCCTCAG 
ATGATGGAAA ACTTTGTGGA GAAGCTGCTG CTGGCCTGGA CTGAGGTGGG GCTGCCCCTC 
CAAGATGTGT CTGTGGCTGC ATGCAACTTC TGTCATCGTC CTGTGCACTT TGAACTTATG 
AGCGAGTGGG AGCGATCCTA CTTTGGGAAC ATGGGACCCC AATATGTTAC CACCTATGCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular dynamics analysis of the structural and dynamic properties of the functionally enhanced hepta-variant of mouse 5-aminolevulinate synthase.
Journal of biomolecular structure & dynamics36(1)152-165(2018 Jan)
Na I,DeForte S,Stojanovski BM,Ferreira GC,Uversky VN

Intron 1 GATA site enhances ALAS2 expression indispensably during erythroid differentiation.
Nucleic acids research45(2)657-671(2017 Jan)
Zhang Y,Zhang J,An W,Wan Y,Ma S,Yin J,Li X,Gao J,Yuan W,Guo Y,Engel JD,Shi L,Cheng T,Zhu X

Murine erythroid 5-aminolevulinate synthase: Truncation of a disordered N-terminal extension is not detrimental for catalysis.
Biochimica et biophysica acta1864(5)441-52(2016 May)
Stojanovski BM,Breydo L,Uversky VN,Ferreira GC

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Alas2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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